CN103444305B - A kind of method of the cultivation impelling miracle fruit seed numerous soon - Google Patents

A kind of method of the cultivation impelling miracle fruit seed numerous soon Download PDF

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CN103444305B
CN103444305B CN201310357795.7A CN201310357795A CN103444305B CN 103444305 B CN103444305 B CN 103444305B CN 201310357795 A CN201310357795 A CN 201310357795A CN 103444305 B CN103444305 B CN 103444305B
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seed
soil
cosan
miracle fruit
temperature
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CN103444305A (en
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吴志刚
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Suzhou Rencheng Bio-Science & Technology Co Ltd
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Abstract

The invention discloses a kind of method of the cultivation impelling miracle fruit seed numerous soon, the step such as transplanting mainly through the sterilization of the choosing of seed, seed, ultrasonic process, the broken dormancy process of seed, the sprouting of seed and soil management, seedling realizes; Utilize growth regulator to control and intermittent warming etc. breaks seed dormant technology, comprises the growth regulator and suitable intermittent warming matrix and variations in temperature that have employed suitable concn, substantially reduces the time of miracle fruit seed germination.The present invention utilizes ultrasonic process seed, and takes suitable temperature and light according to process, and germination rate, germination vigor and germination index are all improved, adopt suitable seedling medium, soil scienceization manages, and improve soil fertility, Pest management rate, plant percent is also very high.

Description

A kind of method of the cultivation impelling miracle fruit seed numerous soon
Technical field
The invention belongs to field of plant cultivation, be specifically related to a kind ofly remove miracle fruit dormancy and improve the method for survival rate of seedling.
Background technology
Miracle fruit belongs to Sapotaceae mystery and belongs to, and is typical tropical evergreen shrubs, original producton location in West Africa, Ghana, the Congo one is with, be also found in Indonesian jungle.The sixties in 20th century, when Premier Zhou Enlai accesses to West Africa, Republic of Ghana gives premier Zhou using miracle fruit as state's gift.After this, miracle fruit starts to cultivate in China.Miracle fruit is a kind of rare species of national treasure level, no matter is in West Africa various countries or China, is all protected, embargo.
Why mysterious miracle fruit is, and reason is that it can change the sense of taste of people, so people ate any acid again food after eating miracle fruit all feels it is sweet.Originally, our tongue there is a lot of taste bud, the taste such as sour, sweet, bitter, peppery, salty can be felt respectively.After having eaten miracle fruit, the function of the taste bud receptor on tongue has temporarily been upset by that glycoprotein, is temporarily benumbed the taste bud receptor of tart flavour sensitivity, inhibits, and excited, active to the taste bud receptor of sweet-sensitive.Miracle fruit can be eaten raw, and what also can be made into acid food helps food agent, spoiled dose that makes the sweet taste that diabetes patient needs.Miracle fruit or interesting ornamental plants, should in the subtropics of China's high temperature, high humidity, torrid areas plantation.
The methods such as miracle fruit is sent out with seed, cutting, air layering are bred, based on slotting kind, do not have scientific soil management pattern, miracle fruit poor growth, after general plantation, 3-4 is just yielded positive results, and the survival rate of seed is not high, seriously constrain the breeding of miracle fruit.
Summary of the invention
The technical problem that the present invention mainly solves is the dormancy adopting multiple method to break seed, improves reproduction speed and the germination rate of seed, and improves survival rate of seedling, soil fertility, Pest management rate.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A method for the cultivation impelling miracle fruit seed numerous soon, is characterized in that, comprise the following steps:
(1) the choosing of seed: the fruit choosing lovely luster, growth and maturity, through drying after a while, removing shrivelled pericarp, picking out full grains, glossy seed is for subsequent use;
(2) the disinfecting of seed: by the seed chosen first first with clear water rinse 1-2 all over after to put into temperature be 20 DEG C of concentration is 20-30g/L KNO 3solution soaked after 2-3 hour, then used 40% sulfuric acid trisodium, 10 times of solution seed soaking 5-6 hour, then filtered and removed described thimerosal; Use seed 2-3 time described in sterile water wash again, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic process: by disinfect miracle fruit seed pack with 3 layers of gauze, put into ultrasonic cleaner ultrasonic process 10-50min, during process, temperature fluctuation controls within 3 DEG C;
(4) seed broke dormancy process: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through running water 2-3 hour, be soaked in 10-40mg/L salicylic acid solution again, soak time is 2-4 h, mix with the wet sand of moisture 10-15% again, be placed on husky in 4 DEG C of refrigerators Tibetan 40 days, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then after moving to 25 DEG C of thermostatic chamber sand Tibetan 40d, soaks 12h, then hide 30 days 4 DEG C of sand at low temperature with 0.4%ABA;
(5) sprouting of seed: by treated seed, put into culture dish, bottom paving double-layer filter paper in culture dish, there is the culture matrix configured in advance on filter paper upper berth, be positioned in 23-25 DEG C of greenhouse and carry out seed germination, every 3d changes 1 filter paper, and every 6d changes No. 1 culture dish, drip distilled water in right amount as required, keep the moisture of matrix at 40-50%; Controlled light intensity is 1500-1700LX, and light application time is 14 hours/day, and temperature is 23-25 DEG C, and cultivate about 10 days, seed sprouting, bears seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
(6) soil management: soil management: soil matrix manages: regulate soil pH value to be 7.0 ~ 8.2, use after mixing with 5 times of volume fine earths after Cosan is sieved, except regulating soil pH value with Cosan, Cosan is then better with peat composed of rotten mosses mixing application effect, or use pine needle to coordinate Cosan to improve soil, wherein pine needle, original soil, muck, Cosan is according to ratio of weight and number 2:3:1:2, or apply saw in soil not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw not, the peat composed of rotten mosses, the ratio of weight and number of liver moss and Cosan is 1:1:1:2,
Increase soil organic matter content: the field planting ditch digging wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, organic matter uses the acid peat composed of rotten mosses, if first January more than must be stacked with sawdust, bark, become thoroughly decomposed completely after decomposing and re-use, as employment domestic animals manure become thoroughly decomposed or straw leaf rot to become thoroughly decomposed after the natural fertilizers that formed, note not mixing ash, lime alkaline matter, content reaches more than 3% ~ 5%, to increase the permeability of soil;
Soil covers: can carry out after soil covers seedling planting, by covering uniform fold at bed surface, wide 1 meter, thick 5 ~ 10 centimetres, cover 2 cm thicks more every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, the nitrogenous fertilizer of 50% need be enriched, rot the sawdust decomposed, nitrogen fertilizer amount should correspondingly reduce, cover unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard of drip irrigation facility is being had to apply, cover unregistered land film and cover the organic matter better or front weed killer herbicide of field planting of effect that combines and kill weeds, entirely cultivate field after field planting and sow green manure Trifolium repense, the leguminous plants such as red clover, field can be covered completely after March, soil fertility can be increased again,
(7) transplanting of seedling: transplant seedlings in the soil of above-mentioned steps (5) and keep temperature about 25 DEG C, after relative moisture 70-75%, 10-15 days, grows the seedling of 8-15 centimetre.
The invention has the beneficial effects as follows:
(1) the present invention utilizes ultrasonic process seed, improves germination rate and the germination index of seed.
(2) soak with 55% sulfuric acid solution, this process can eliminate the inhibition of seed coat to a certain extent, makes seed fully absorb moisture, too increase the gas permeability of exosper simultaneously, reduce the restriction of oxygen exchange, make the oxidized decomposition of mortifier, enhance merismatic activation capacity.
(3) utilize growth regulator to control and intermittent warming etc. breaks seed dormant technology, comprises the growth regulator and suitable intermittent warming matrix and variations in temperature that have employed suitable concn, substantially reduces the time of miracle fruit seed germination.
(4) in the seed germination stage, take suitable temperature and light according to process, germination rate, germination vigor and germination index are all improved.
(5) in seedling replanting, adopt rational culture matrix, seedling growth is vigorous, and kind shoot survival percent is high; Increase soil fertility, Pest management rate.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
embodiment 1
A method for the cultivation impelling miracle fruit seed numerous soon, realizes in the following manner:
Choose the fruit of lovely luster, growth and maturity, through drying after a while, removing shrivelled pericarp, picking out full grains, glossy seed is for subsequent use; To put into temperature after the seed chosen first first is rinsed 1 time with clear water be 20 DEG C of concentration is 20g/L KNO 3after solution soaks 3 hours, then use 40% sulfuric acid trisodium, 10 times of solution seed soakings 5 hours, then filter and remove described thimerosal; Use seed described in sterile water wash 2 times again, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
By disinfect miracle fruit seed pack with 3 layers of gauze, put into ultrasonic cleaner ultrasonic process 10min, during process, temperature fluctuation controls within 3 DEG C; By above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through running water 2 hours, be soaked in 10mg/L salicylic acid solution again, soak time is 4 h, mix with the wet sand of moisture 10% again, be placed on husky in 4 DEG C of refrigerators Tibetan 40 days, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then after moving to 25 DEG C of thermostatic chamber sand Tibetan 40d, soaks 12h, then hide 30 days 4 DEG C of sand at low temperature with 0.4%ABA;
By treated seed, put into culture dish, bottom paving double-layer filter paper in culture dish, there is the culture matrix configured in advance on filter paper upper berth, be positioned in 23 DEG C of greenhouses and carry out seed germination, every 3 d change 1 filter paper, and every 6 d change No. 1 culture dish, drip distilled water in right amount as required, keep the moisture of matrix 40%; Controlled light intensity is 1500LX, and light application time is 14 hours/day, and temperature is 23 DEG C, and cultivate about 10 days, seed sprouting, bears seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
Transplant seedlings to seedbed, seedbed is made up of two-layer matrix, overlayer matrix be 10 cm thicks by volume for detritus soil: vermiculite: rice chaff: perlite=2: the mixture of 2: 1: 1, lower floor is garden mould and the river sand of 10 cm thicks, tpn spray solution with 0.2% is in the matrix stirred, carry out sterilization processing, and covered with sunshade net, avoid being subject to strong illumination, keep temperature about 25 DEG C, relative moisture, after 70%, 10-15 day, grows the seedling of 8-15 centimetre.
Through above-mentioned process, the seed germination rate of miracle fruit is 92%, and germination vigor is 92%, and germination index is 56.3, and plant percent is 92%.
embodiment 2
A method for the cultivation impelling miracle fruit seed numerous soon, specific as follows:
Choose the fruit of lovely luster, growth and maturity, through drying after a while, removing shrivelled pericarp, picking out full grains, glossy seed is for subsequent use; To put into temperature after the seed chosen first first is rinsed 2 times with clear water be 20 DEG C of concentration is 30g/L KNO 3after solution soaks 2 hours, then use 40% sulfuric acid trisodium, 10 times of solution seed soakings 6 hours, then filter and remove described thimerosal; Use seed described in sterile water wash 3 times again, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
By disinfect miracle fruit seed pack with 3 layers of gauze, put into ultrasonic cleaner ultrasonic process 50min, during process, temperature fluctuation controls within 3 DEG C; By above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through running water 3 hours, be soaked in 40mg/L salicylic acid solution again, soak time is 2h, mix with the wet sand of moisture 15% again, be placed on husky in 4 DEG C of refrigerators Tibetan 40 days, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then after moving to 25 DEG C of thermostatic chamber sand Tibetan 40d, soaks 12h, then hide 30 days 4 DEG C of sand at low temperature with 0.4%ABA;
By treated seed, put into culture dish, bottom paving double-layer filter paper in culture dish, there is the culture matrix configured in advance on filter paper upper berth, be positioned in 25 DEG C of greenhouses and carry out seed germination, every 3 d change 1 filter paper, and every 6 d change No. 1 culture dish, drip distilled water in right amount as required, keep the moisture of matrix 50%; Controlled light intensity is 1700LX, and light application time is 14 hours/day, and temperature is 25 DEG C, and cultivate about 10 days, seed sprouting, bears seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
Transplant seedlings to seedbed, seedbed is made up of two-layer matrix, overlayer matrix be 15 cm thicks by volume for detritus soil: vermiculite: rice chaff: perlite=2: the mixture of 2: 1: 1, lower floor is garden mould and the river sand of 7 cm thicks, tpn spray solution with 0.2% is in the matrix stirred, carry out sterilization processing, and covered with sunshade net, avoid being subject to strong illumination, keep temperature about 25 DEG C, relative moisture, after 75%, 10-15 day, grows the seedling of 8-15 centimetre.
Through above-mentioned process, the seed germination rate of miracle fruit is 95%, and germination vigor is 93%, and germination index is 57.6, and plant percent is 93%.
embodiment 3
A method for the cultivation impelling miracle fruit seed numerous soon, specific as follows:
Choose the fruit of lovely luster, growth and maturity, through drying after a while, removing shrivelled pericarp, picking out full grains, glossy seed is for subsequent use; Then to put into temperature after the seed chosen first first being rinsed 2 times with clear water be 20 DEG C of concentration is 25g/L KNO 3after solution soaks 3 hours, then use 40% sulfuric acid trisodium, 10 times of solution seed soakings 6 hours, then filter and remove described thimerosal; Use seed described in sterile water wash 3 times again, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic process: by disinfect miracle fruit seed pack with 3 layers of gauze, put into ultrasonic cleaner ultrasonic process 30min, during process, temperature fluctuation controls within 3 DEG C;
(4) seed broke dormancy process: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through running water 3 hours, be soaked in 25mg/L salicylic acid solution again, soak time is 3 h, mix with the wet sand of moisture 13% again, be placed on husky in 4 DEG C of refrigerators Tibetan 40 days, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then after moving to 25 DEG C of thermostatic chamber sand Tibetan 40d, soaks 12h, then hide 30 days 4 DEG C of sand at low temperature with 0.4%ABA;
(5) sprouting of seed: by treated seed, put into culture dish, bottom paving double-layer filter paper in culture dish, there is the culture matrix configured in advance on filter paper upper berth, be positioned in 24 DEG C of greenhouses and carry out seed germination, every 3 d change 1 filter paper, and every 6 d change No. 1 culture dish, drip distilled water in right amount as required, keep the moisture of matrix 45%; Controlled light intensity is 1600LX, and light application time is 14 hours/day, and temperature is 24 DEG C, and cultivate about 10 days, seed sprouting, bears seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
(6) transplanting of seedling: transplant seedlings to seedbed, seedbed is made up of two-layer matrix, overlayer matrix be 13 cm thicks by volume for detritus soil: vermiculite: rice chaff: perlite=2: the mixture of 2: 1: 1, lower floor is garden mould and the river sand of 8 cm thicks, tpn spray solution with 0.2% is in the matrix stirred, carry out sterilization processing, and covered with sunshade net, avoid being subject to strong illumination, keep temperature about 25 DEG C, relative moisture, after 73%, 10-15 day, grows the seedling of 8-15 centimetre.
Embodiment 4: a kind of method of the cultivation impelling miracle fruit seed numerous soon, comprises the following steps:
(1) the choosing of seed: the fruit choosing lovely luster, growth and maturity, through drying after a while, removing shrivelled pericarp, picking out full grains, glossy seed is for subsequent use;
(2) the disinfecting of seed: by the seed chosen first first with clear water rinse 1-2 all over after to put into temperature be 20 DEG C of concentration is 20-30g/L KNO 3solution soaked after 2-3 hour, then used 40% sulfuric acid trisodium, 10 times of solution seed soaking 5-6 hour, then filtered and removed described thimerosal; Use seed 2-3 time described in sterile water wash again, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic process: by disinfect miracle fruit seed pack with 3 layers of gauze, put into ultrasonic cleaner ultrasonic process 10-50min, during process, temperature fluctuation controls within 3 DEG C;
(4) seed broke dormancy process: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through running water 2-3 hour, be soaked in 10-40mg/L salicylic acid solution again, soak time is 2-4 h, mix with the wet sand of moisture 10-15% again, be placed on husky in 4 DEG C of refrigerators Tibetan 40 days, stirred 1 time every 3 days, keep seed moistening.Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then after moving to 25 DEG C of thermostatic chamber sand Tibetan 40d, soaks 12h, then hide 30 days 4 DEG C of sand at low temperature with 0.4%ABA;
(5) sprouting of seed: by treated seed, put into culture dish, bottom paving double-layer filter paper in culture dish, there is the culture matrix configured in advance on filter paper upper berth, be positioned in 23-25 DEG C of greenhouse and carry out seed germination, every 3d changes 1 filter paper, and every 6d changes No. 1 culture dish, drip distilled water in right amount as required, keep the moisture of matrix at 40-50%; Controlled light intensity is 1500-1700LX, and light application time is 14 hours/day, and temperature is 23-25 DEG C, and cultivate about 10 days, seed sprouting, bears seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite :=2:1:1;
(6) soil management: soil management: soil matrix manages: regulate soil pH value to be 7.0 ~ 8.2, use after mixing with 5 times of volume fine earths after Cosan is sieved, except regulating soil pH value with Cosan, Cosan is then better with peat composed of rotten mosses mixing application effect, or use pine needle to coordinate Cosan to improve soil, wherein pine needle, original soil, muck, Cosan is according to ratio of weight and number 2:3:1:2, or apply saw in soil not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw not, the peat composed of rotten mosses, the ratio of weight and number of liver moss and Cosan is 1:1:1:2,
Increase soil organic matter content: the field planting ditch digging wide 50 cm, dark 40 cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, organic matter uses the acid peat composed of rotten mosses, if first January more than must be stacked with sawdust, bark, become thoroughly decomposed completely after decomposing and re-use, as employment domestic animals manure become thoroughly decomposed or straw leaf rot to become thoroughly decomposed after the natural fertilizers that formed, note not mixing ash, lime alkaline matter, content reaches more than 3% ~ 5%, to increase the permeability of soil;
Soil covers: can carry out after soil covers seedling planting, by covering uniform fold at bed surface, wide 1 meter, thick 5 ~ 10 centimetres, cover 2 cm thicks more every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, the nitrogenous fertilizer of 50% need be enriched, rot the sawdust decomposed, nitrogen fertilizer amount should correspondingly reduce, cover unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard of drip irrigation facility is being had to apply, cover unregistered land film and cover the organic matter better or front weed killer herbicide of field planting of effect that combines and kill weeds, entirely cultivate field after field planting and sow green manure Trifolium repense, the leguminous plants such as red clover, field can be covered completely after March, soil fertility can be increased again,
(7) transplanting of seedling: transplant seedlings in the soil of above-mentioned steps (5) and keep temperature about 25 DEG C, after relative moisture 70-75%, 10-15 days, grows the seedling of 8-15 centimetre.
Through above-mentioned process, the seed germination rate of miracle fruit is 98%, and germination vigor is 96%, and germination index is plant percent is 99%.And plant strain growth is vigorous, improve soil fertility 10.2%, Disease management rate 21%.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.

Claims (1)

1. a method for the cultivation impelling miracle fruit seed numerous soon, is characterized in that, comprise the following steps:
(1) the choosing of seed: the fruit choosing lovely luster, growth and maturity, through drying after a while, removing shrivelled pericarp, picking out full grains, glossy seed is for subsequent use;
(2) the disinfecting of seed: by the seed chosen first with clear water rinse 1-2 all over after to put into temperature be 20 DEG C of concentration is 20-30g/L KNO 3solution soaked after 2-3 hour, then used 40% sulfuric acid trisodium, 10 times of solution seed soaking 5-6 hour, then filtered and removed described thimerosal; Use seed 2-3 time described in sterile water wash again, continue to filter and remove residual described thimerosal and described sterile water, obtain sterile seed;
(3) ultrasonic process: by disinfect miracle fruit seed pack with 3 layers of gauze, put into ultrasonic cleaner ultrasonic process 10-50min, during process, temperature fluctuation controls within 3 DEG C;
(4) seed broke dormancy process: by above-mentioned miracle fruit seed first in 55% sulfuric acid solution 3 hours, then through running water 2-3 hour, be soaked in 10-40mg/L salicylic acid solution again, soak time is 2-4h, mix with the wet sand of moisture 10-15% again, be placed on husky in 4 DEG C of refrigerators Tibetan 40 days, stirred 1 time every 3 days, keep seed moistening
Then take out and screen out sand, thin layer dries in the shade, then soaks 24h with 0.5%GA, then after moving to 25 DEG C of thermostatic chamber sand Tibetan 40d, soaks 12h, then hide 30 days 4 DEG C of sand at low temperature with 0.4% ABA;
(5) sprouting of seed: by treated seed, put into culture dish, bottom paving double-layer filter paper in culture dish, there is the culture matrix configured in advance on filter paper upper berth, be positioned in 23-25 DEG C of greenhouse and carry out seed germination, every 3d changes 1 filter paper, and every 6d changes No. 1 culture dish, drip distilled water in right amount as required, keep the moisture of matrix at 40-50%; Controlled light intensity is 1500-1700LX, and light application time is 14 hours/day, and temperature is 23-25 DEG C, and cultivate 10 days, seed sprouting, bears seedling; In described culture matrix, the mixing quality ratio of each composition is sand: vermiculite: perlite=2:1:1;
(6) soil management: regulate soil pH value to be 7.0 ~ 8.2, use after mixing with 5 times of volume fine earths after Cosan is sieved, except regulating soil pH value with Cosan, Cosan is then better with peat composed of rotten mosses mixing application effect, or use pine needle to coordinate Cosan to improve soil, wherein pine needle, original soil, muck, Cosan are according to ratio of weight and number 2:3:1:2, or apply in soil saw not, the peat composed of rotten mosses, liver moss and Cosan carry out soil melioration, wherein saw not, the peat composed of rotten mosses, liver moss and Cosan ratio of weight and number be 1:1:1:2;
Increase soil organic matter content: the field planting ditch digging wide 50cm, dark 40cm, organic matter, fertilizer, original soil are improved the soil in the ratio of 1:1:1, organic matter uses the acid peat composed of rotten mosses, if first January more than must be stacked with sawdust, bark, become thoroughly decomposed completely after decomposing and re-use, as employment domestic animals manure become thoroughly decomposed or straw leaf rot to become thoroughly decomposed after the natural fertilizers that formed, note not mixing ash, lime alkaline matter, content reaches more than 3% ~ 5%, to increase the permeability of soil;
Soil covers: can carry out after soil covers seedling planting, by covering uniform fold at bed surface, wide 1 meter, thick 5 ~ 10 centimetres, cover 2 cm thicks more every year later, to keep original thickness, the new sawdust decomposed if application is not rotted, the nitrogenous fertilizer of 50% need be enriched, rot the sawdust decomposed, nitrogen fertilizer amount should correspondingly reduce, cover unregistered land film and can prevent soil water evaporation, control weeds, improve ground temperature, preferably the orchard of drip irrigation facility is being had to apply, cover unregistered land film and cover the organic matter better or front weed killer herbicide of field planting of effect that combines and kill weeds, entirely cultivate field after field planting and sow green manure Trifolium repense, the leguminous plants such as red clover, field can be covered completely after March, soil fertility can be increased again,
(7) transplanting of seedling: transplant seedlings in the soil of above-mentioned steps (5) and keep temperature 25 DEG C, after relative moisture 70-75%, 10-15 days, grows the seedling of 8-15 centimetre.
CN201310357795.7A 2013-08-16 2013-08-16 A kind of method of the cultivation impelling miracle fruit seed numerous soon Expired - Fee Related CN103444305B (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1545836A (en) * 2003-12-02 2004-11-17 中国科学院西双版纳热带植物园 Artificial grow seedling method for mysterious fruit
CN102266111A (en) * 2011-08-22 2011-12-07 中国热带农业科学院农产品加工研究所 Husk and kernel separating method for fresh fruits of synsepalum dulcificum
CN103238388A (en) * 2013-05-24 2013-08-14 常熟市润丰农业有限公司 Method for scientifically managing soil of newly built blueberry garden
CN103238447A (en) * 2013-05-24 2013-08-14 常熟市润丰农业有限公司 High-quality high-efficiency high-yield blueberry cultivation method
CN103238400A (en) * 2013-05-31 2013-08-14 常熟市佳盛农业科技发展有限公司 Method for removing seed dormancy of tapiscia sinensis oliv and improving seedling survival rate

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008266877A (en) * 2008-06-27 2008-11-06 Hollywood Magic:Kk Wig

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1545836A (en) * 2003-12-02 2004-11-17 中国科学院西双版纳热带植物园 Artificial grow seedling method for mysterious fruit
CN102266111A (en) * 2011-08-22 2011-12-07 中国热带农业科学院农产品加工研究所 Husk and kernel separating method for fresh fruits of synsepalum dulcificum
CN103238388A (en) * 2013-05-24 2013-08-14 常熟市润丰农业有限公司 Method for scientifically managing soil of newly built blueberry garden
CN103238447A (en) * 2013-05-24 2013-08-14 常熟市润丰农业有限公司 High-quality high-efficiency high-yield blueberry cultivation method
CN103238400A (en) * 2013-05-31 2013-08-14 常熟市佳盛农业科技发展有限公司 Method for removing seed dormancy of tapiscia sinensis oliv and improving seedling survival rate

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