CN103212162A - Mixing photostimulation method and mixing photostimulation device - Google Patents
Mixing photostimulation method and mixing photostimulation device Download PDFInfo
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Abstract
Disclosed are a mixing photostimulation method and a mixing photostimulation device. The method comprises the following steps: providing a light emitting diode (LED) light source, wherein the LED light source is a combined light source of a yellow light LED and a red light LED; and enabling the LED light source to illuminate onto a main body so as to promote collagen synthesis. The illumination of the yellow light LED is 1000-3500 luxes, and the illumination of the red light LED is 6000-9500 luxes. The ratio of the number of the yellow light LEDs and the number of the red light LEDs is 0.5-2 to 0.5-2.
Description
Technical field
The invention relates to a kind of mixed light stimulating method and mixed light stimulating apparatus, especially refer to a kind of mixed light stimulating method and mixed light stimulating apparatus that promotes collagen protein synthesis.
Background technology
In department of dermatologry diagnosis, often with Drug therapy patient skin disease, as comedo etc.; but for a long time, the result of Drug therapy is accompanied by side effect more, takes the burden that can cause on the body metabolism for a long time; and therapeutic effect is desirable not to the utmost, and regular meeting's recurrence can't effectively improve patient's skin problem.
In recent years, the medical and beauty treatment industry is prosperous day by day, research is pointed out: wavelength can be used for the comedo treatment between the blue light of 400nm to 475nm, because of blue light can act on single-phase oxygen of toxigenicity and free radical with sclererythrin (coproporphyrin) in the light sensation in Propiobacterium (Propionibacterium acnes) or the histiocyte, and then destroy antibacterial and part sebaceous gland histiocyte, improve the inflamed of comedo.On the other hand, wavelength between the HONGGUANG of 600nm to 750nm, wavelength between the gold-tinted of 550nm to 600nm, and wavelength between the green glow of 500nm to 570nm, can stimulate the fibroblast of skin corium, and then promote collagen protein synthesis, prevent skin aging.
But, for reaching above-mentioned effect, industry is used laser or pulsed light mostly at present, and therefore the energy or the intensity of two kinds of light just are enough to reach above-mentioned effect, but cause cell injury easily.Recently just general light source of develop actively or LED source replace above-mentioned high-intensity light source, but at present LED source since energy a little less than, urgent need will find suitable illuminance just to be enough to be effective, otherwise light illumination is crossed and low can can't be brought into play curative effect; Otherwise, when light illumination is too high, except meeting cause cell impaired, device volume is promoted, can't develop the little and lightweight Portable phototherapy device of volume, and employed light source is all single kind of light source.
In view of the above, if can send out a kind of mixed light stimulating method of output and mixed light stimulating apparatus, wherein utilize the light emitting diode combined of different colours and specific illumination range, reach the lifting collagen protein synthesis, and can save manpower and time cost, make the patient can have beautiful skin fast.
Summary of the invention
The object of the present invention is to provide a kind of mixed light stimulating method.
Another object of the present invention is to provide a kind of mixed light stimulating apparatus.
For achieving the above object, mixed light stimulating method provided by the invention may further comprise the steps:
One LED source is provided, and this LED source is the combined light source of a Yellow light emitting diode and a red light-emitting diode; And
This LED source is shone in a main body, to promote collagen protein synthesis, wherein, the illumination of this Yellow light emitting diode is 1000 to 3500 luxs (Lux), the illumination of this red light-emitting diode is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2.
Described mixed light stimulating method, wherein, this main body is a fibroblast, a macrophage or its combination.
Described mixed light stimulating method, wherein, the optical wavelength range of this Yellow light emitting diode is between between the 570nm to 590nm, and the optical wavelength range of this red light-emitting diode is between 620nm to 750nm.
Described mixed light stimulating method, wherein, the irradiation time of this LED source was between 5 minutes to 90 minutes.
Mixed light stimulating apparatus provided by the invention comprises:
One housing forms an accommodation space and has an end face and a lateral margin, and this end face is provided with a light-emitting window;
One scattering sheet covers this light-emitting window of this housing;
One first light source module, it is arranged in this accommodation space of this housing and has one first light emitting diode, this first light emitting diode is located at this scattering sheet below, and this first light emitting diode is the combined light source of a Yellow light emitting diode and a red light-emitting diode, wherein, the illumination of this Yellow light emitting diode is 1000 to 3500 luxs, the illumination of this red light-emitting diode is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2; And
One control module, it electrically connects this first light source module and a power module.
Described mixed light stimulating apparatus, wherein, this power module is in an external power source or this accommodation space that is arranged at this housing.
Described mixed light stimulating apparatus, wherein, this control module comprises: a charging hole electrically connects this control module for this power module.
Described mixed light stimulating apparatus, wherein, this control module comprises: an on and off switch is arranged at this surface of shell, to control this power module supply power supply.
Described mixed light stimulating apparatus, wherein, this lateral margin of this housing is provided with a light hole.
Described mixed light stimulating apparatus wherein, comprising: a light transmission piece covers this light hole; And a secondary light source module, corresponding light transmission piece setting also emits beam and passes this light transmission piece.
Described mixed light stimulating apparatus, wherein, this control module comprises: a mode selector switch all is arranged at this surface of shell, to start this first light source module or this secondary light source module.
The present invention adopts and sends the red light-emitting diode of specific illumination range and the hybrid light source of Yellow light emitting diode, in the hope of reaching the stimulation fibroblast, increases collagen protein synthesis, simultaneously blood circulation promoting, the old useless cellular metabolism of quickening
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention one mixed light stimulating apparatus
Fig. 2 is the side view of the embodiment of the invention one mixed light stimulating apparatus
Fig. 3 is the system block diagrams of the embodiment of the invention one mixed light stimulating apparatus.
Fig. 4 shows the human fibroblast survival rate and the collagen protein synthesis rate of the embodiment of the invention two.
Fig. 5 shows the collagen protein synthesis rate of the human fibroblast of per unit in the embodiment of the invention two.
Fig. 6 shows the human fibroblast survival rate and the collagen protein synthesis rate of the embodiment of the invention three.
Fig. 7 shows the collagen protein synthesis rate of the human fibroblast of per unit in the embodiment of the invention three.
Primary clustering symbol description in the accompanying drawing:
First light source module, 40, the first light emitting diodes 41;
Secondary light source module 50, the second light emitting diodes 51.
The specific embodiment
An aspect of the present invention provides a kind of mixed light stimulating method, may further comprise the steps: a LED source is provided, and this LED source is the combined light source of a Yellow light emitting diode and a red light-emitting diode; And this LED source shone in a main body, to promote collagen protein synthesis, wherein, the illumination of this Yellow light emitting diode is 1000 to 3500 luxs (1ux), the illumination of this red light-emitting diode is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2.
Known technology uses different wavelength of laser or pulsed light to reach comedo treatment usually, stimulates the fibroblast of skin corium to promote collagen protein synthesis, but because of laser or pulsed light intensity is high and equipment is huge, ordinary consumer is difficult to have.Recently, though the light emitting diode of use is arranged as light source, expectation can reach the effect of above-mentioned lifting collagen protein, but owing to known technology research is not arranged at the influence of the illumination of lumination of light emitting diode and hybrid light source for cell, because of not setting under the prerequisite of specific illumination, it is unknown whether known method can reach the real genus of above-mentioned effect.Review, the method for the invention utilizes the light emitting diode that sends gold-tinted and HONGGUANG as hybrid light source, and it is defined in different illumination ranges, and it is hereby ensured to reach stimulates fibroblast to promote collagen protein synthesis.
Therefore, when shining, can stimulate macrophage (macrophage) to discharge cytohormone (cytokine), promote the fibroblast division with the hybrid light source of suitable illuminance HONGGUANG and Yellow light emitting diode and lasting appropriate time; (fibroblast growth factor FGF), and then increases collagen protein synthesis also can to stimulate simultaneously fibroblast synthetic DNA and secretion somatomedin.If this main body is cells in vivo, fibroblast or the macrophage in the skin corium for example, direct printing opacity then, irradiation skin reaches promotion wound healing and antidotal effect; Perhaps, if this main body is a cell in vitro, then can more treated cell be planted the object of bringing back to life and reach above-mentioned effect earlier through after the above-mentioned processing.Hence one can see that, and main body of the present invention is meant the object that is subjected to light stimulation.
In the above-mentioned mixed light stimulating method of the present invention, this main body is preferably a fibroblast, a macrophage or its combination.In the present invention's one preferred embodiments, this main body is a fibroblast.In addition, the optical wavelength range of this Yellow light emitting diode can be between between the 570nm to 590nm, and the optical wavelength range of this red light-emitting diode can be between 620nm to 750nm.In addition, the hybrid light source of this HONGGUANG and Yellow light emitting diode, on irradiation time, there is not particular restriction, as long as can reach above-mentioned effect and can not damage to this main body, can adjust to some extent according to the predetermined luminous intensity of this red light-emitting diode and this Yellow light emitting diode emitted light, when illumination is higher, just can just reach same effect with short irradiation time; Otherwise when illumination was low, then available long irradiation time reached identical effect.
For example, send at red light-emitting diode under the illuminance of sending 1000 to 3500 luxs of 6000 to 9500 luxs and Yellow light emitting diode, irradiation time can be between 5 minutes to 90 minutes.If be higher than above-mentioned illumination range,, get off for a long time and can cause cell impaired because of illumination is too high though do not have too much influence in the short time; Otherwise, if illumination is low excessively, even long-time use also is difficult to effectively be effective.
Mixed light stimulating apparatus provided by the invention, wherein adopt and send the red light-emitting diode of specific illumination range and the hybrid light source of Yellow light emitting diode, in the hope of reaching the stimulation fibroblast, increase collagen protein synthesis, simultaneously blood circulation promoting, the old useless cellular metabolism of quickening.
Another aspect of the present invention provides a kind of mixed light stimulating apparatus, comprising: a housing, and form an accommodation space and have an end face and a lateral margin, this end face is provided with a light-emitting window; One scattering sheet covers this light-emitting window of this housing; One first light source module, it is arranged in this accommodation space of this housing and has one first light emitting diode, this first light emitting diode is located at this scattering sheet below, and this first light emitting diode is the combined light source of a Yellow light emitting diode and a red light-emitting diode, wherein, the illumination of this Yellow light emitting diode is 1000 to 3500 luxs (lux), the illumination of this red light-emitting diode is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2; And a control module, it electrically connects this first light source module and a power module.
From the above, combination is sent the light emitting diode of gold-tinted and HONGGUANG as hybrid light source in the mixed light stimulating apparatus of the present invention, and the light of different colours all is defined in corresponding illumination range, therefore after mixed light stimulating apparatus irradiation of the present invention, can stimulate fibroblast, guarantee that collagen protein synthesis promotes.
In the above-mentioned mixed light stimulating apparatus of the present invention, this power module can be an external power source or is arranged in this accommodation space of this housing.If this power module is arranged in this accommodation space of this housing, it can comprise chargeable battery or can hold general aneroid battery or minicell, reaches the effect of supply power supply.On the other hand, if power module is an external power source or for being arranged at the chargeable battery in this accommodation space of this housing, this control module can have optionally and comprises: a charging hole electrically connects this control module for this power module.
In addition, in the above-mentioned mixed light stimulating apparatus of the present invention, this control module also can be had and optionally comprised: an on and off switch is arranged at this surface of shell, to control this power module supply power supply.In addition, this housing is preferably by the low material of light transmittance and is constituted, as reflexive height or the high material of density, to reduce the light leakage phenomena of mixed light stimulating apparatus.In addition, this area personage also can increase the integrally-built adaptation of mixed light stimulating apparatus by various structural designs, to reduce the light leakage phenomena of mixed light stimulating apparatus.
On the other hand, in the above-mentioned mixed light stimulating apparatus of the present invention, this lateral margin alternative of this housing is provided with a light hole.In the case, the mixed light stimulating apparatus can also comprise: a light transmission piece covers this light hole; And the corresponding light transmission piece setting of a secondary light source module and emit beam and pass this light transmission piece.In the case, this control module also can comprise again: a mode selector switch, all be arranged at this surface of shell, and to start this first light source module or this secondary light source module, in other words, promptly switch the start between first light source module and the secondary light source module.In addition, the light emitting diode that is adopted in this first light source module and this secondary light source module can be same color or different colours.
In the above-mentioned mixed light stimulating apparatus of the present invention, be located at this scattering sheet of this light-emitting window, can help even bright dipping, avoid diagnosis and treatment light direct irradiation user eyes, and the uniformity of raising device photostimulation effect, in other words be about to originally belong to the light emitting diode of point source, after the astigmatism effect, form area source at the light-emitting window place; In addition, be located at this light transmission piece of this light hole, then not necessarily be required to be scattering sheet, if scattering sheet then can reach above-mentioned effect, if not be scattering sheet, the light that then can directly transmit point source and provided.
In the above-mentioned mixed light stimulating apparatus of the present invention, this first light source module and this secondary light source module can be designed to replaceable, in other words use red light-emitting diode and Yellow light emitting diode to form this first light source module and this secondary light source module.If when needing the HONGGUANG of higher proportion to shine, then increase the quantity of red light-emitting diode in the light emitting module.In addition, employed led designs in this first light source module and this secondary light source module can also be become replaceable, in other words if need higher proportion gold-tinted when irradiation, then the light emitting diode on the light source module is changed into Yellow light emitting diode.
In sum, mixed light stimulating method of the present invention and mixed light stimulating apparatus can be by adopting the light emitting diode of different colours, the for example combination of HONGGUANG and Yellow light emitting diode, carry out Photic Stimulation, therefore can reach the lifting collagen protein synthesis, and then reach the aging-resistant effect.
Below be that the personage who has the knack of this skill can understand other advantages of the present invention and effect easily by the content that this description disclosed by particular specific embodiment explanation embodiments of the present invention.The present invention also can be implemented or be used by other different specific embodiments, and the every details in this description also can be carried out various modifications and change based on different viewpoints and application under not departing from spirit of the present invention.
Those accompanying drawings are the sketch map of simplification in the embodiments of the invention.Only those icons only show the assembly relevant with the present invention, and the aspect that its shown assembly is non-when be actual enforcement, component count, the shape equal proportion during its actual enforcement are one optionally to design, and its assembly layout kenel may be more complicated.
Embodiment one
Referring to figs. 1 to Fig. 3, wherein Fig. 1 is the structural representation of mixed light stimulating apparatus of the present invention, and Fig. 2 is the side view of mixed light stimulating apparatus of the present invention, and Fig. 3 is the system block diagrams of mixed light stimulating apparatus of the present invention.
As shown in Figure 1 to Figure 3, mixed light stimulating apparatus of the present invention comprises: a housing 10, a light transmission piece 13, a scattering sheet 14, one first light source module 40, a secondary light source module 50 and a control module 30.
This housing 10 forms an accommodation space, can hold each module.In addition, this housing 10 has an end face 11 and a lateral margin 12, and this end face 11 is provided with a light-emitting window 111, and this lateral margin 12 is provided with a light hole 121.
Be positioned at these light-emitting window 111 places of this end face 11, use this scattering sheet 14 to cover; Be positioned at this light hole 121 of this lateral margin 12, use this light transmission piece 13 to cover.In addition, these secondary light source module 50 corresponding light transmission piece 13 are arranged at this accommodation space of this housing 10, and emit beam and pass this light transmission piece 13, and have at least one second light emitting diode 51.In this, if only being used for printing opacity merely, this light transmission piece 13 is used for astigmatism, then 50 of secondary light source modules become point source.
This first light source module 40 is arranged in this accommodation space of this housing 10 and has a plurality of first light emitting diodes 41 that array is arranged, this first light emitting diode 41 is located at this scattering sheet 14 belows, and this first light emitting diode 41 is combined light sources of red light-emitting diode and Yellow light emitting diode, wherein, the light illumination that this Yellow light emitting diode sends through this scattering sheet 14 is 1000 to 3500 luxs (lux), the light illumination that this Yellow light emitting diode sends through this scattering sheet 14 is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2.In present embodiment, this Yellow light emitting diode is 1: 1 with the quantity ratio of red light-emitting diode.
This control module 30 electrically connects this first light source module 40 and a power module 20, and this control module 30 comprises: a charging hole 33 electrically connects this control module 30 for this power module 20; One on and off switch 31 is arranged at this housing 10 surfaces, to control this power module 20 supply power supplys; And a mode selector switch 32, all be arranged at this housing 10 surfaces, to start this first light source module 40 or this secondary light source module 50.
This power module 20 can be an external power source or is arranged in this accommodation space of this housing 10.In this power module 20 was arranged at this accommodation space of this housing 10, this power module 20 can contain chargeable battery or can hold general aneroid battery or minicell, reached the effect of supply power supply.
Therefore, above-mentioned mixed light stimulating apparatus adopts and sends the red light-emitting diode of specific illumination range and the hybrid light source of Yellow light emitting diode, just can reach the stimulation fibroblast, increase collagen protein synthesis, simultaneously blood circulation promoting, the old useless cellular metabolism of quickening.
Embodiment two
Utilizing light emitting diode to send illumination is 7, and the HONGGUANG of 800lux is inquired into it for the influence of human fibroblast survival rate with the secretion collagen protein.
At first, will contain the DMEM cell culture fluid of human fibroblast, and add in the 48 hole culture plates, the cell number of being inoculated is 2 * 10
4Individual/hole, the common 0.5ml of the cumulative volume of culture fluid (containing cell) in each hole places CO in 48 holes
2Incubator was cultivated 24 hours.Afterwards, take out whole culture fluid, add PBS buffer 0.5ml again, and using red light-emitting diode (Lux 7,800) irradiation after 5,10,15,30 minutes, whole PBS buffer adds the culture fluid of 0.5ml again in the taking-up hole, cultivate 24 hours again, and repeat once above-mentioned photostimulation step.
Then, the MTT reagent of culture medium 0.5ml that more renews and adding 0.125ml is put to 37 ℃, 5%CO
2Cell culture incubator internal reaction after 4 hours, whole culture medium are taken out, add the DMSO dissolving first of 0.5ml
(formazan), get and utilize ELISA trace dish analyser in 0.2ml to 96 hole, measure its light absorption value when OD570nm.Being calculated as of cell survival rate: cell survival rate (%)=(OD behind the irradiation
570/ control group OD
570) * 100%, wherein control group are meant the cell that does not use the irradiation of mixed light stimulating apparatus.
In addition, for the collagen protein context of detection, at first will contain the DMEM cell culture fluid of human fibroblast, and add in the 48 hole culture plates, the cell number of being inoculated is 2 * 10
4Individual/hole, the common 0.5ml of the cumulative volume of culture fluid (containing cell) in each hole places CO in 48 holes
2Incubator was cultivated 24 hours.Afterwards, take out whole culture fluid, add PBS buffer 0.5ml again, and using red light-emitting diode (Lux 7,800) irradiation after 5,10,15,30 minutes, whole PBS buffer adds the culture fluid of 0.5ml again in the taking-up hole, cultivate 24 hours again, and repeat once above-mentioned photostimulation step.
Then, culture medium in the hole is all taken out, and put into the 1.5ml centrifuge tube, the hole of cultivating cell afterwards distinctly adds the aqueous acetic acid of 0.5ml 0.5M (4 ℃), after placing dissolvings in 20 minutes collagen protein wherein, take out aqueous solutions whole in the hole and put into the 1.5ml centrifuge tube, centrifuge tube successively adds 50 μ l acid neutralizing agent (acid neutralizing reagent more respectively, Biocolor), the separation concentrating agents of 4 ℃ of 100 μ l (Isolation ﹠ Concentration Reagent, and in 4 ℃ of refrigerators, place and spend the night Biocolor).Afterwards, centrifuge tube taken out and centrifugal 10 minutes with 12000rpm, remove supernatant, in centrifuge tube, add 1ml photoghraphic coupler (Sircol Dye Reagent again, Biocolor) add in the centrifuge tube and shook 30 minutes, again with 12000rpm after centrifugal 10 minutes, remove supernatant, add again 4 ℃ of 750 μ l the hydrochlorate abluent (Acid-Salt Wash Reagent, Biocolor), again with 12000rpm after centrifugal 10 minutes, remove supernatant, centrifuge tube add again 250 μ l alkaline agents (Alkali Reagent, Biocolor), take out 200 μ l in last every pipe and add in 96 porose discs, measure the light absorption value of 555nm.At collagen protein production rate (%)=(the collagen protein growing amount behind the irradiation/control group collagen protein growing amount) * 100%, wherein control group is meant the cell that does not use the irradiation of mixed light stimulating apparatus.
Above-mentioned experimental result is with reference to figure 4 and Fig. 5, and wherein Fig. 4 is that illumination is 7, after the red light-emitting diode of 800lux shines human fibroblast, and cell survival rate and collagen protein production rate; Fig. 5 is the collagen protein production rate of irradiation back per unit cell.As shown in Figure 4, be presented at irradiation after 5 minutes, the effect that promotes fibroblast propagation is promptly arranged, and the content of collagen protein also increases and increases along with cell quantity.In addition, as shown in Figure 5, though make the collagen protein growing amount of per unit cell reduce because of fibroblast quantity propagation improves at the beginning as can be known, along with irradiation time increases, the collagen protein growing amount of per unit fibroblast then promotes gradually.
Embodiment three
Utilize light emitting diode to send the gold-tinted of illumination, inquire into it for of the influence of human fibroblast survival rate with the secretion collagen protein for 2290lux.
At first, will contain the DMEM cell culture fluid of human fibroblast, and add in the 48 hole culture plates, the cell number of being inoculated is 2 * 10
4Individual/hole, the common 0.5ml of the cumulative volume of culture fluid (containing cell) in each hole places CO in 48 holes
2Incubator was cultivated 24 hours.Afterwards, take out whole culture fluid, add PBS buffer 0.5ml again, and use Yellow light emitting diode (Lux 2290) irradiation after 5,10,15,30 minutes, whole PBS buffer adds the culture fluid of 0.5ml again in the taking-up hole, cultivates 24 hours again, and repeats once above-mentioned photostimulation step.
Then, the MTT reagent of culture medium 0.5ml that more renews and adding 0.125ml is put to 37 ℃, 5%CO
2Cell culture incubator internal reaction after 4 hours, whole culture medium are taken out, add the DMSO dissolving first of 0.5ml
(formazan), get and utilize ELISA trace dish analyser in 0.2ml to 96 hole, measure its light absorption value when OD570nm.Being calculated as of cell survival rate: cell survival rate (%)=(OD behind the irradiation
570/ control group OD
570) * 100%, wherein control group are meant the cell that does not use the irradiation of mixed light stimulating apparatus.
In addition, for the collagen protein context of detection, at first will contain the DMEM cell culture fluid of human fibroblast, and add in the 48 hole culture plates, the cell number of being inoculated is 2 * 10
4Individual/hole, the common 0.5ml of the cumulative volume of culture fluid (containing cell) in each hole places CO in 48 holes
2Incubator was cultivated 24 hours.Afterwards, take out whole culture fluid, add PBS buffer 0.5ml again, and use Yellow light emitting diode (Lux 2290) irradiation after 5,10,15,30 minutes, whole PBS buffer adds the culture fluid of 0.5ml again in the taking-up hole, cultivates 24 hours again, and repeats once above-mentioned photostimulation step.
Then, culture medium in the hole is all taken out, and put into the 1.5ml centrifuge tube, the hole of cultivating cell afterwards distinctly adds the aqueous acetic acid of 0.5ml 0.5M (4 ℃), after placing dissolvings in 20 minutes collagen protein wherein, take out aqueous solutions whole in the hole and put into the 1.5ml centrifuge tube, centrifuge tube successively adds 50 μ l acid neutralizing agent (acid neutralizing reagent more respectively, Biocolor), separation concentrating agents (the Isolation﹠Concentration Reagent of 4 ℃ of 100 μ l, and in 4 ℃ of refrigerators, place and spend the night Biocolor).Afterwards, centrifuge tube taken out and centrifugal 10 minutes with 12000rpm, remove supernatant, in centrifuge tube, add 1ml photoghraphic coupler (Sircol Dye Reagent again, Biocolor) add in the centrifuge tube and shook 30 minutes, again with 12000rpm after centrifugal 10 minutes, remove supernatant, add again 4 ℃ of 750 μ l the hydrochlorate abluent (Acid-Salt Wash Reagent, Biocolor), again with 12000rpm after centrifugal 10 minutes, remove supernatant, centrifuge tube add again 250 μ l alkaline agents (Alkali Reagent, Biocolor), take out 200 μ l in last every pipe and add in 96 porose discs, measure the light absorption value of 555nm.At collagen protein production rate (%)=(the collagen protein growing amount behind the irradiation/control group collagen protein growing amount) * 100%, wherein control group is meant the cell that does not use the irradiation of mixed light stimulating apparatus.
Above-mentioned experimental result is with reference to figure 6 and Fig. 7, and wherein Fig. 6 is that illumination is after the Yellow light emitting diode of 2290lux shines human fibroblast, cell survival rate and collagen protein production rate; Fig. 7 is the collagen protein production rate of irradiation back per unit cell.As shown in Figure 6, be presented at irradiation after 5 minutes, the effect that promotes fibroblast propagation is promptly arranged, and the content of collagen protein also increases and increases along with cell quantity.In addition, as shown in Figure 7, through shining about 5 to 10 minutes, the collagen protein growing amount of per unit cell is promoted to the highest as can be known.
The foregoing description is only given an example for convenience of description, and the interest field that the present invention advocated is from should but not only limiting to the foregoing description with described being as the criterion of claim scope of application.
Claims (11)
1. mixed light stimulating method may further comprise the steps:
One LED source is provided, and this LED source is the combined light source of a Yellow light emitting diode and a red light-emitting diode; And
This LED source is shone in a main body, to promote collagen protein synthesis, wherein, the illumination of this Yellow light emitting diode is 1000 to 3500 luxs, the illumination of this red light-emitting diode is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2.
2. mixed light stimulating method as claimed in claim 1, wherein, this main body is a fibroblast, a macrophage or its combination.
3. mixed light stimulating method as claimed in claim 2, wherein, the optical wavelength range of this Yellow light emitting diode is between between the 570nm to 590nm, and the optical wavelength range of this red light-emitting diode is between 620nm to 750nm.
4. mixed light stimulating method as claimed in claim 3, wherein, the irradiation time of this LED source was between 5 minutes to 90 minutes.
5. mixed light stimulating apparatus comprises:
One housing forms an accommodation space and has an end face and a lateral margin, and this end face is provided with a light-emitting window;
One scattering sheet covers this light-emitting window of this housing;
One first light source module, it is arranged in this accommodation space of this housing and has one first light emitting diode, this first light emitting diode is located at this scattering sheet below, and this first light emitting diode is the combined light source of a Yellow light emitting diode and a red light-emitting diode, wherein, the illumination of this Yellow light emitting diode is 1000 to 3500 luxs, the illumination of this red light-emitting diode is 6000 to 9500 luxs, and the quantity of this Yellow light emitting diode and this red light-emitting diode is than being 0.5-2: 0.5-2; And
One control module, it electrically connects this first light source module and a power module.
6. mixed light stimulating apparatus as claimed in claim 5, wherein, this power module is in an external power source or this accommodation space that is arranged at this housing.
7. mixed light stimulating apparatus as claimed in claim 6, wherein, this control module comprises: a charging hole electrically connects this control module for this power module.
8. mixed light stimulating apparatus as claimed in claim 5, wherein, this control module comprises: an on and off switch is arranged at this surface of shell, to control this power module supply power supply.
9. mixed light stimulating apparatus as claimed in claim 5, wherein, this lateral margin of this housing is provided with a light hole.
10. mixed light stimulating apparatus as claimed in claim 9 wherein, comprising: a light transmission piece covers this light hole; And a secondary light source module, corresponding light transmission piece setting also emits beam and passes this light transmission piece.
11. mixed light stimulating apparatus as claimed in claim 10, wherein, this control module comprises: a mode selector switch all is arranged at this surface of shell, to start this first light source module or this secondary light source module.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039303A (en) * | 2015-08-20 | 2015-11-11 | 吴剑波 | Method for promoting activation and differentiation of cells |
| CN113750372A (en) * | 2021-09-02 | 2021-12-07 | 北京翌光医疗科技研究院有限公司 | Flexible mask |
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Application publication date: 20130724 |