CN103131688B - Preparation method for corncob and/or straw bearing photosynthetic bacteria and application thereof - Google Patents
Preparation method for corncob and/or straw bearing photosynthetic bacteria and application thereof Download PDFInfo
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- CN103131688B CN103131688B CN201310002638.4A CN201310002638A CN103131688B CN 103131688 B CN103131688 B CN 103131688B CN 201310002638 A CN201310002638 A CN 201310002638A CN 103131688 B CN103131688 B CN 103131688B
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- straw
- corn cob
- corncob
- photosynthetic bacterium
- appendix
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Abstract
The invention relates to a preparation method for a corncob and/or a straw bearing photosynthetic bacteria and an application thereof. The method comprises the following steps: (1) washing the corncob and/or the straw, and conducting high temperature sterilization on the corncob and/or the straw to obtain a sterilized corncob and/or a straw; (2) placing the sterilized corncob and/or the straw into a mixed liquid for a first soaking, and conducting air drying to obtain an air-dried corncob and/or a straw; (3) placing the air-dried corncob and/or the straw into a photosynthetic bacteria culture solution for a secondary soaking, taking the corncob and/or the straw out, and storing the corncob and/or the straw in a sealed mode or throwing the corncob and/or the straw into an eutrophicated lake directly. With the subsidiary agricultural plant products, namely, the corncob and/or the straw as raw materials and as a carbon source of the photosynthetic bacteria at the same time, the preparation method for the corncob and/or the straw bearing the photosynthetic bacteria and the application thereof are attached with a preparation method of the photosynthetic bacteria and applied in the absorption of phosphorus and nitrogen in eutrophicated water, and can remarkably improve purification efficiency of water quality and prolong the service life of the photosynthetic bacteria.
Description
Technical field
The present invention relates to a kind of corn cob of appendix photosynthetic bacterium and/or the preparation method of stalk and application thereof; be specifically related to the preparation method of a kind of plant by-product-corn cob and/or stalk appendix photosynthetic bacterium; and for the improvement of eutrophic lake water quality, belong to environmental protection technical field.
Background technology
Along with deepening continuously of process of industrialization, global environmental pollution destroys the eubiosis that global biosphere was formed over several hundred million years day by day, and constitutes a threat to the living environment of mankind itself.Particularly rivers, lake and basin, coastal waters are due to the phosphorus that discharges in a large number and nitrogen, cause body eutrophication, and big area causes breaking out of blue-green algae or red tide, then causes water hypoxia, grows unwanted aquatic organism, cause the poisoning even harm humans of fish healthy.Most undressed municipal effluent enters urban lakes, river, and Water phosphorus is polluted and is on the rise, its direct result is body eutrophication.According to statistics, the lake of China more than 80% is polluted, and many lakes have not reached III class water quality standard.The lake of domestic each big and medium-sized cities and periphery, due to the continuous expansion of city size, the growth of population and the fast development of economy, the discharge of large amount of sewage industrial and agricultural wastewater, eutrophication problem is more and more serious, many lakes are close to eutrophy, and what have even reaches extremely eutrophic degree.The swift and violent increase of the macronutrient particularly element such as N, P, destroy the nutritive equilibrium that water body is original, cause the amount reproduction of algae, this is the most notable feature of body eutrophication.
Along with phosphorus and nitrogen are more and more serious to the harm of water body, people come to realise the importance that sewage removes nitrogen, phosphorus.The principal character of lake pollution is body eutrophication, and the key eliminating lake eutrophication is to cut down N, P of water body in lake and the load of bed mud organic carbon and N, P, eliminate the basis of Measures of Algae in Water Body overgrowing, reach and reduce Measures of Algae in Water Body biomass, improve the object of water transparency.Cut down the technological approaches of water body in lake N, P and organic carbon except eliminating point source (dam source of pollution), minimizing and Control the Plan Pollution Sources the most basic this kind of approach, modal method has mechanical desilting method, diversion irrigation and biological restoration.It is also be effectively the approach of more traditional reduction Lakes in Nutrition element that machinery desilting and diversion are rinsed, but these two kinds of methods exist two problems: one is that cost is high, and two is fundamentally to control and to eliminate lake eutrophication, just shifts it.
Photosynthetic bacterium be a class under anaerobism illumination condition, carbonic acid gas or low molecule organic matter can be utilized to carry out the hydrosphere microorganism of photoheterotrophy growth as photosynthetic electron acceptor(EA); Under dark aerobic condition, organism can be utilized to carry out aerobic or heterotrophic growth as respiratory substrate; The photosynthesis of oxygen can not be put.Photosynthetic bacterium has that to cultivate raw material sources wide, low price, and growth conditions easily controls, and thalline can the advantage such as recycle; Obtain in the process of agricultural, aquaculture and high concentrated organic wastewater in recent years and apply comparatively widely.After the positive Thailand of Japanese scholars holt waits the waste water successfully processed fecaluria and food, starch, leather and bean product to process, photosynthetic bacterium is widely used in wastewater treatment.It can be used for administering the blue-green algae in lake, and its action principle is exactly consumed by the organism in water body, also can absorb the phosphorus in water and nitrogen simultaneously, reduces the content of phosphorus, nitrogen, closes and makes the growth of blue-green algae lack nutrition and dead.Under aerobic condition, with the photosynthetic bacterium process waste water of free state, but in extensive wastewater treatment process, but there are problems in usual people.As somatic cells is easily washed away by flowing water, though under hydrostatic condition also may engulf by other biological, therefore utilization ratio is low, and solid-liquid separation difficulty, thalline not easily recycles and reuses.
Therefore, it is inconvenient that photosynthetic bacterium also exists liquid bacterial transport in improvement lake blue algae process, sheds time-consuming; And thalline is easily washed away by flowing water in water body, concentration is easily diluted, and causes the utilization ratio of administering eutrophic water low, the shortcomings such as DeGrain.
Summary of the invention
It is low that technical problem to be solved by this invention is to provide a kind of preparation cost, the corn cob of appendix photosynthetic bacterium easy and simple to handle, killing blue algae efficiency is high and/or the preparation method of stalk and application thereof.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of corn cob of appendix photosynthetic bacterium and/or the preparation method of stalk, comprise the following steps:
1) corn cob and/or stalk are cleaned, and then carry out high-temperature sterilization process, object kills the miscellaneous bacteria on corn cob and/or stalk, obtains the corn cob after sterilizing and/or stalk;
2) the high-temperature sterilization process of corn cob and/or stalk:
Corn cob after the sterilizing obtained in step 1) and/or stalk are put into mixed solution to carry out first time and soak, and then carry out air-dry, obtain air-dry after corn cob and/or stalk,
Wherein, described mixed solution comprises the raw material of following mass percent concentration: the chitosan of 0.1 ~ 1%; The sodium alginate of 1 ~ 5%; The K of 1 ~ 5%
2hPO
4;
3) surface treatment of appendix thing:
By above-mentioned air-dry after corn cob and/or stalk put into again photosynthetic bacterium bacterial classification solution carry out second time soak, then take out, carry out Seal and preservation or directly dropped in the lake of eutrophication.
The invention has the beneficial effects as follows: the present invention with agriculture plant by-product-corn cob and/or stalk for raw material, simultaneously also as the carbon source of photosynthetic bacterium, the preparation method of appendix photosynthetic bacterium; And the absorption of the phosphorus be applied in eutrophic water and nitrogen, purification of water quality efficiency can be significantly improved and extend bacterium duration of service.
On the basis of technique scheme, the present invention can also do following improvement.
Further, described stalk also comprised the steps: stalk to cut out before cleaning, and punched.
Further, described length of being carried out cutting out by stalk is 40 ~ 50cm; The described instrument carrying out punching is the mace of band nail.
Further, described stalk is maize straw.
Further, the raw number of described photosynthetic bacterium is 2.0 × 10
9~ 3.1 × 10
10cfu/mL.
Further, the formula of the substratum of described photosynthetic bacterium is: at 1m
3tap water in add sodium bicarbonate 5.5kg, sodium-acetate 3kg, yeast extract paste 0.5kg, K
2hPO
40.25kg, KH
2pO40.25kg, NH
4cl0.2kg, NaCl0.2kg, MgSO40.2kg, (NH
4)
2sO
40.15kg.
Further, in step 1), described in carry out high-temperature sterilization process processing condition be: corn cob and/or stalk are placed in closed high-temperature sterilization tank, are 0.1 ~ 0.5MPa at pressure, and temperature is under the condition of 110 DEG C, sterilising treatment 1 ~ 3 hour.
Further, in step 2) in, described in carry out first time soak time be 1 ~ 12 hour; It is described that to carry out the air-dry time be 1 ~ 5 hour.
Further, in step 3), described in carry out second time soak time be 1 ~ 6 hour; The processing condition of described Seal and preservation are: Seal and preservation under the condition of-5 ~ 20 DEG C.
Another technical scheme that the present invention solves the problems of the technologies described above is as follows: the corn cob of a kind of appendix photosynthetic bacterium and/or stalk are administering the application on lake blue algae, be specially: corn cob and/or the stalk rope of the appendix photosynthetic bacterium of above-mentioned preparation method being prepared are fixed on floating log, and suspend in water, after 3 ~ 10 days, shake off the dead blue-green algae that its surface adhesion.
When corn cob and/or the reduction of stalk algae killing effect of appendix photosynthetic bacterium, take out the water surface, by step 2) and 3) carry out regenerating.
Corn cob and/or the stalk rope of appendix photosynthetic bacterium are fixed on floating log, and suspend in water, and object is for absorbing phosphorus in water and nitrogen, making blue-green algae lose nutrient, controls the growth of blue-green algae until death.
Further, described stalk is every 5 ~ 20 and is bundled into a bundle rope and is fixed on floating log.
Embodiment
Be described principle of the present invention and feature below, example, only for explaining the present invention, is not intended to limit scope of the present invention.
The corn cob of appendix photosynthetic bacterium and/or a preparation method for stalk, comprise the following steps:
1) corn cob and/or stalk being cleaned, be then placed in closed high-temperature sterilization tank, is 0.1 ~ 0.5MPa at pressure, and temperature is under the condition of 110 DEG C, and sterilising treatment 1 ~ 3 hour, obtains the corn cob after sterilizing and/or stalk;
2) corn cob after the sterilizing obtained in step 1) and/or stalk are put into mixed solution to carry out first time and soak 1 ~ 12 hour, and then carry out air-dry 1 ~ 5 hour, obtain air-dry after corn cob and/or stalk,
Wherein, described mixed solution comprises the raw material of following mass percent concentration: the chitosan of 0.1 ~ 1%; The sodium alginate of 1 ~ 5%; The K of 1 ~ 5%
2hPO
4;
3) corn cob after air-dry and/or stalk being immersed in aerobic plate count amount is 2.0 × 10
9~ 3.1 × 10
10in the photosynthetic bacterium bacterial classification solution of cfu/mL 1 ~ 6 hour, then take out, under the condition of-5 ~ 20 DEG C, carry out Seal and preservation or directly dropped in the lake of eutrophication.
Described stalk also comprised before cleaning that to be cut out by stalk be 40 ~ 50 centimetres, and the step of mace punching with band nail.
The corn cob of the appendix photosynthetic bacterium prepared and/or stalk rope are fixed on floating log, and suspend in water, after 3 ~ 10 days, shake off the dead blue-green algae that its surface adhesion, when corn cob and/or the reduction of stalk algae killing effect of appendix photosynthetic bacterium, take out the water surface, by step 2) and 3) regenerate.
Below by way of several specific embodiment to illustrate the present invention.
Embodiment 1
Corn cob is cleaned airing, is placed in the high-temperature sterilization tank sealing treatment 2 hours of 0.2MPa; Take out and be immersed in the chitosan that mass percent concentration is 0.5%, the sodium alginate of 0.5% and the K of 3%
2hPO
4mixing solutions in, soak 12 hours, take out air-dry 5 hours, be then immersed in aerobic plate count amount 2.0 × 10
9~ 3.1 × 10
10in the photosynthetic bacterium solution of cfu/mL 6 hours; Then be concatenated with rope and be fixed on floating log, throwing in the phosphorus and nitrogen that absorb in water in the lake of eutrophication, making blue-green algae lose nutrient and dead, reaching the object in pollution administration lake.Shake off its surface adsorption after 3 days and dead blue-green algae, when the corn cob algae killing effect of appendix photosynthetic bacterium reduces, the appendix step of photosynthetic bacterium can be carried out again, make it regenerate.
The corn cob 1 getting the appendix photosynthetic bacterium obtained in the present embodiment 1 puts into 3m
3park landscape pond in, the depth of water 1.0 meters is tested, temperature at 25 ~ 30 DEG C, through 5 days process after, water sampling detect, comparing result is as follows:
Upper table illustrates: the blue-green algae lake water upper strata of the corn cob process of the appendix photosynthetic bacterium provided by the embodiment of the present invention 1 is clarified substantially, visual inspection is without blue-green algae, chlorophyll a clearance just reaches 78.11%, algae number clearance has 95.76%, water quality transparency brings up to 45cm by 10cm, and BOD, COD, TN, TP have remarkable reduction in addition.As can be seen here, blue-green algae Be very effective removed by material prepared by the present invention, instant effect.
Embodiment 2
Cut out by maize straw, cutting out rear every root maize straw length is 40 ~ 50 centimetres, and with mace punching, cleans airing, be placed in 0.5MPa high-temperature sterilization tank sealing treatment 3 hours.Take out and be immersed in the chitosan that mass percent concentration is 0.5%, the sodium alginate of 0.5% and the K of 3%
2hPO
4mixing solutions in.Soak 8 hours, take out air-dry 3 hours, (aerobic plate count amount is 2.0 × 10 to be then immersed in photosynthetic bacterium
9~ 3.1 × 10
10cfu/mL) in solution 1 hour; Then be concatenated with rope, and be fixed on floating log, throw in the lake of eutrophication, kill blue-green algae, the lake of pollution administration.Shake off its surface adsorption after 10 days and dead blue-green algae.When the maize straw algae killing effect of appendix photosynthetic bacterium reduces, the appendix step can carrying out photosynthetic bacterium again makes it regenerate.
It is (separate with fence) in the lake of 1200 square metres that the maize straw 50 of the appendix photosynthetic bacterium obtained in Example 2 puts into place's area, the depth of water 1.5 meters is tested, temperature at 25 ~ 30 DEG C, through 7 days process after, water sampling detects, and comparing result is as follows:
Upper table illustrates: the blue-green algae lake water upper strata of the maize straw process of the appendix photosynthetic bacterium provided by the embodiment of the present invention 2 is clarified substantially, visual inspection is without blue-green algae, blue-green algae number clearance has 97.42%, chlorophyll a clearance just reaches 96.36%, water quality transparency brings up to 65cm by 12cm, and BOD, COD, TN, TP have remarkable reduction in addition.As can be seen here, blue-green algae Be very effective removed by material prepared by the present invention, instant effect.
Embodiment 3
Cleaned by maize straw after corn cob and punching, airing, is placed in 0.1MPa high-temperature sterilization tank sealing treatment 1 hour.Take out and be immersed in the chitosan that mass percent concentration is 0.5%, the sodium alginate of 0.5% and the K of 3%
2hPO
4mixing solutions in, soak 1 hour, take out air-dry 1 hour, be then immersed in photosynthetic bacterium aerobic plate count amount 2.0 × 10
9~ 3.1 × 10
10cfu/mL) in solution 4 hours; Taking-up rope is concatenated, and is fixed on floating log, throws in the lake of eutrophication, kills blue-green algae, the lake of pollution administration.Shake off its surface adsorption after 7 days and dead blue-green algae.When corn cob and the reduction of maize straw algae killing effect of appendix photosynthetic bacterium, the appendix step of photosynthetic bacterium can be carried out again, make it regenerate.
It is (separate with fence) in the lake of 800 square metres that the corn cob of the appendix photosynthetic bacterium obtained in Example 3 and maize straw 20 put into place's area, the depth of water 1.8 meters is tested, temperature at 25 ~ 30 DEG C, through 7 days process after, water sampling detects, and comparing result is as follows:
Upper table illustrates: the corn cob of the appendix photosynthetic bacterium provided by the embodiment of the present invention 3 and the blue-green algae lake water upper strata of maize straw process are clarified substantially, visual inspection is without blue-green algae, chlorophyll a clearance just reaches 95.22%, and algae number clearance has 97.02%, and water quality transparency brings up to 55cm by 8cm; BOD, COD, TN, TP have significantly reduction as can be seen here in addition, and blue-green algae Be very effective removed by material prepared by the present invention, administer eutrophic water instant effect.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the corn cob of appendix photosynthetic bacterium and/or a preparation method for straw, is characterized in that, comprise the following steps:
1) corn cob and/or straw are cleaned, and then carry out high-temperature sterilization process, obtain the corn cob after sterilizing and/or straw; Described processing condition of carrying out high-temperature sterilization process are: corn cob and/or straw are placed in closed high-temperature sterilization tank, are 0.1 ~ 0.5MPa at pressure, and temperature is under the condition of 110 DEG C, sterilising treatment 1 ~ 3 hour;
2) by step 1) in corn cob after the sterilizing that obtains and/or straw put into mixed solution and carry out first time and soak, and then carry out air-dry, obtain air-dry after corn cob and/or straw,
Wherein, described mixed solution comprises the raw material of following mass percent concentration: the chitosan of 0.1 ~ 1%; The sodium alginate of 1 ~ 5%; The K of 1 ~ 5%
2hPO
4; The described time of carrying out first time immersion is 1 ~ 12 hour; It is described that to carry out the air-dry time be 1 ~ 5 hour;
3) by above-mentioned air-dry after corn cob and/or straw put into again photosynthetic bacterium bacterial classification solution carry out second time soak, then take out, carry out Seal and preservation or directly dropped in the lake of eutrophication; The described time of carrying out second time immersion is 1 ~ 6 hour; The processing condition of described Seal and preservation are: Seal and preservation under the condition of-5 ~ 20 DEG C.
2. the preparation method of corn cob according to claim 1 and/or straw appendix photosynthetic bacterium, is characterized in that: described straw also comprised the steps: straw to cut out before cleaning, and punched.
3. the preparation method of corn cob according to claim 2 and/or straw appendix photosynthetic bacterium, is characterized in that: described length of being carried out cutting out by straw is 40 ~ 50cm; The described instrument carrying out punching is the mace of band nail.
4. the preparation method of corn cob according to claim 3 and/or straw appendix photosynthetic bacterium, is characterized in that: described straw is corn stalk.
5. the preparation method of corn cob according to claim 1 and/or straw appendix photosynthetic bacterium, is characterized in that: described photosynthetic bacterium aerobic plate count amount is 2.0 × 10
9~ 3.1 × 10
10cfu/mL; The formula of the substratum of described photosynthetic bacterium is: at 1m
3tap water in add sodium bicarbonate 5.5kg, sodium-acetate 3kg, yeast extract paste 0.5kg, K
2hPO
40.25kg, KH
2pO
40.25kg, NH
4cl 0.2kg, NaCl 0.2kg, MgSO
40.2kg, (NH
4)
2sO
40.15kg.
6. the corn cob of an appendix photosynthetic bacterium and/or straw are administering the application on lake blue algae, it is characterized in that: corn cob and/or the straw rope of the appendix photosynthetic bacterium of such as any one of claim 1 to 5 preparation method being prepared are fixed on floating log, and suspend in water, after 3 ~ 10 days, shake off the dead blue-green algae that its surface adhesion.
7. the corn cob of appendix photosynthetic bacterium according to claim 6 and/or straw are administering the application on lake blue algae, it is characterized in that: described straw is every 5 ~ 20 and is bundled into a bundle rope and is fixed on floating log.
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|---|---|---|---|
| CN201310002638.4A CN103131688B (en) | 2013-01-05 | 2013-01-05 | Preparation method for corncob and/or straw bearing photosynthetic bacteria and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310002638.4A CN103131688B (en) | 2013-01-05 | 2013-01-05 | Preparation method for corncob and/or straw bearing photosynthetic bacteria and application thereof |
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| CN103131688B true CN103131688B (en) | 2015-04-01 |
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| CN109182399B (en) * | 2018-08-24 | 2021-09-03 | 湖北工业大学 | Immobilized acetic acid fermentation method for improving acetic acid producing capability of acetic acid bacteria |
| CN110697912A (en) * | 2019-11-27 | 2020-01-17 | 上源环工生态环境科技(苏州)有限公司 | Rapid and long-acting water treatment agent and preparation method thereof |
| CN111320287A (en) * | 2020-02-20 | 2020-06-23 | 广东海洋大学深圳研究院 | Method for treating eutrophic seawater by compounding bacteria and chitosan |
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2013
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| CN1978639A (en) * | 2005-12-02 | 2007-06-13 | 南京理工大学 | Method for preparing immobilized thallus and its use |
| CN101130794A (en) * | 2007-08-09 | 2008-02-27 | 天津科技大学 | Production method of immobilized microorganism fermenting propionic acid |
| CN101768583A (en) * | 2010-01-22 | 2010-07-07 | 中国海洋大学 | Method for immobilizing oil degradation bacteria with reed straw as carrier and application thereof |
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