CN103013829B - Structure of deep-well cell culture plate cover - Google Patents
Structure of deep-well cell culture plate cover Download PDFInfo
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- CN103013829B CN103013829B CN201210580641.XA CN201210580641A CN103013829B CN 103013829 B CN103013829 B CN 103013829B CN 201210580641 A CN201210580641 A CN 201210580641A CN 103013829 B CN103013829 B CN 103013829B
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Abstract
The invention provides a structure of a deep-well cell culture plate cover, which can effectively avoid pollution and ensure favorable air permeability among culture plates. The structure comprises a stainless steel plate cover, an anti-pollution membrane, a waterproof membrane and a silica gel plate. The invention is characterized in that two side walls of the stainless steel plate cover are respectively provided with clamping grooves; the anti-pollution membrane, waterproof membrane and silica gel plate are sequentially clamped in the clamping grooves from top to bottom; and four corners of the upper end surface of the stainless steel plate cover are respectively provided with a bulge.
Description
Technical field
The present invention relates to cell culture apparatus technical field in biology, particularly relate to the deep hole culture plate of cell cultures, be specially the structure of deep-well cell culture plate cover.
Background technology
At present, carry out in biology often using culture plate in the earlier process choice of parameters research of high-flux cell cultivation and fermentation engineering amplification process, culture plate has all supported the use plate lid, conventional panels lid is " sandwich " structure, as shown in Figure 1, namely by stainless steel plate lid 1, waterproof membrane 3, anti-soil film 2 and silica-gel plate 4 are formed, during use, by anti-soil film 2, waterproof membrane 3 and silica-gel plate 4 serve as a contrast in the groove of stainless steel plate lid 1 from top to bottom successively, again stainless steel plate lid 1 to be buckled on culture plate 8, then applying external force makes silica-gel plate 4 compress, and be closely adjacent to culture plate 8 and make in culture plate between each culture hole 7 isolated, prevent from the mutual pollution between each culture hole 7 and can reduce in culturing process evaporating.In order to keep anti-soil film 2, waterproof membrane 3 and the silica-gel plate 4 of lining in stainless steel plate lid 1 smooth, the size of anti-soil film 2, waterproof membrane 3 and silica-gel plate 4 is all slightly less than the size of stainless steel plate lid 1 groove, when growth plate lid uses, anti-soil film 2, waterproof membrane 3 and silica-gel plate 4 easily come off in groove, pollute, also due to silica-gel plate 4 forced compression, generation local bending fold, can make not to be adjacent between silica-gel plate 4 and individual culture hole 7, can cause mutually polluting between each culture hole 7; Meanwhile, stainless steel plate lid 1 top is plane, when cell cultures, multiple culture plate 8 is superimposed, and the ventilating pit of lower floor's stainless steel plate lid 1 can be blocked by upper strata culture plate 8 bottom surface, and ventilation property is bad, simultaneously, before use, stainless steel plate lid 1 is inverted and is placed on experiment table, and the ventilating pit 5 of stainless steel plate lid 1 directly contacts experiment table top, easily contaminated, and then causing the pollution of fermented liquid, in Fig. 1,6 is the ventilating pit of silica-gel plate 4.
Summary of the invention
For the problems referred to above, the invention provides the structure of deep-well cell culture plate cover, it can effectively avoid polluting, and ensures good air permeability between culture plate.
Its technical scheme is such, it comprises stainless steel plate lid, anti-soil film, waterproof membrane and silica-gel plate, it is characterized in that: described stainless steel plate lid two side is provided with draw-in groove, described anti-soil film, described waterproof membrane and described silica-gel plate are installed in described draw-in groove from top to bottom successively, and described stainless steel plate covers end face corner and is provided with projection.
It is further characterized in that:
Be provided with bottom described silica-gel plate and the plug one to one of culture hole in culture plate, described plug center is provided with vent-hole, has space between adjacent described plug;
Described projection is truncated rectangular pyramids shape.
After adopting structure of the present invention, its beneficial effect is: anti-soil film, waterproof membrane and silica-gel plate are installed in the draw-in groove of stainless steel plate lid dual-side, effectively prevents anti-soil film, waterproof membrane and silica-gel plate from coming off; The top of stainless steel plate lid is provided with truncated rectangular pyramids shape projection, make bottom the culture plate upper strata culture plate that is stacked together and leave space between lower floor's stainless steel plate lid, guarantee the good air permeability of culture plate, simultaneously, when stainless steel plate lid upside down is on experiment table, truncated rectangular pyramids shape projection can avoid stainless steel plate cover to pollute, and guarantees that fermented liquid is not contaminated; Bottom silica-gel plate, be provided with the plug of each culture hole of corresponding culture plate, can prevent from further mutually polluting between a culture hole.
Accompanying drawing explanation
Fig. 1 is the structural representation of the supporting plate lid of traditional culture plate;
Fig. 2 is structural representation of the present invention;
Fig. 3 be silica-gel plate of the present invention look up structural representation;
Fig. 4 is that the A-A of Fig. 3 is to cross-sectional view;
Fig. 5 be stainless steel plate lid of the present invention look up structural representation;
Fig. 6 is that the C-C of Fig. 5 is to cross-sectional view;
Fig. 7 is that the B-B of Fig. 5 is to cross-sectional view;
Fig. 8 is the plan structure schematic diagram of stainless steel plate lid of the present invention;
Fig. 9 is that the D-D of Fig. 8 is to cross-sectional view.
Embodiment
The present invention is described for the structure of 24 hole deep-well cell culture plate cover, structure of the present invention is not limited to 24 deep hole growth plate lids, be equally applicable to the growth plate lid of 48 hole depth well culture plate plate lids, 96 hole depth well culture plate plate lids and other hole bit quantity, equally, structure of the present invention is applicable to the culture plate cover plate of position, different shapes hole, and its plug shape can change according to concrete culture plate deep hole shape.
As shown in Fig. 2 to Fig. 9, the structure of deep-well cell culture plate cover, it comprises stainless steel plate, 9, anti-soil film 2, waterproof membrane 3 and silica-gel plate 11, stainless steel plate lid 9 two side is provided with draw-in groove 14, anti-soil film 2, waterproof membrane 3 and silica-gel plate 11 are installed in stainless steel plate lid 9 two side draw-in groove 14 from top to bottom successively, corner, stainless steel plate lid 9 upper surface is provided with protruding 10, be provided with bottom silica-gel plate 11 and the plug 12 one to one of culture hole 7 in culture plate 8, plug 12 center is provided with vent-hole 6, has space 13 between adjacent plug; The projection 10 of stainless steel plate lid 9 is truncated rectangular pyramids shape.
Claims (1)
1. the structure of deep-well cell culture plate cover, it comprises stainless steel plate lid, anti-soil film, waterproof membrane and silica-gel plate, it is characterized in that: described stainless steel plate lid two side is provided with draw-in groove, described anti-soil film, described waterproof membrane and described silica-gel plate are installed in described draw-in groove from top to bottom successively, and described stainless steel plate covers end face corner and is provided with projection; Be provided with bottom described silica-gel plate and the plug one to one of culture hole in culture plate, described plug center is provided with vent-hole, has space between adjacent described plug, and described projection is truncated rectangular pyramids shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210580641.XA CN103013829B (en) | 2012-12-28 | 2012-12-28 | Structure of deep-well cell culture plate cover |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210580641.XA CN103013829B (en) | 2012-12-28 | 2012-12-28 | Structure of deep-well cell culture plate cover |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103013829A CN103013829A (en) | 2013-04-03 |
| CN103013829B true CN103013829B (en) | 2015-04-22 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210580641.XA Active CN103013829B (en) | 2012-12-28 | 2012-12-28 | Structure of deep-well cell culture plate cover |
Country Status (1)
| Country | Link |
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| CN (1) | CN103013829B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642690B (en) * | 2013-12-11 | 2015-09-02 | 江南大学 | The plate lid structure of Tissue Culture Plate |
| CN104371923B (en) * | 2014-12-09 | 2017-04-05 | 江南大学 | Tissue Culture Plate and its plate lid |
| CN104371921B (en) * | 2014-12-09 | 2017-04-05 | 江南大学 | The press-loading apparatus of multi-layer cellular culture plate |
| CN110023480B (en) * | 2016-12-14 | 2022-11-11 | 株式会社日立高新技术 | Culture instrument |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5238082A (en) * | 1975-09-13 | 1977-03-24 | Heraeus Gmbh W C | Cultivating container for microorganism * bacterium and tissue |
| BR112012008309A2 (en) * | 2009-09-05 | 2016-03-15 | Lonza Biologics Plc | deep well plate system with lid |
| CN102268369A (en) * | 2010-06-03 | 2011-12-07 | 中国航天员科研训练中心 | Gas exchange type cell culture bottle |
| CN203065490U (en) * | 2012-12-28 | 2013-07-17 | 江南大学 | Structure of cover of deep hole cell culture plate |
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2012
- 2012-12-28 CN CN201210580641.XA patent/CN103013829B/en active Active
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| Publication number | Publication date |
|---|---|
| CN103013829A (en) | 2013-04-03 |
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