CN102423021A - Nutrient jelly containing microalgae DHA (Docosahexaenoic Acid) and preparation method thereof - Google Patents
Nutrient jelly containing microalgae DHA (Docosahexaenoic Acid) and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a nutrient jelly containing microalgae DHA (Docosahexaenoic Acid) and a preparation method thereof. The nutrient jelly comprises the following components of 0.002-0.0105 percent of the microalgae DHA, 0.20-0.50 percent of hydrophilic colloid, 10.0-15.0 percent of sweetening agent, 0.25-0.50 percent of acidity regulator, 0.02-0.05 percent of preservative, 0.005-0.012 percent of antioxidant and 0.1-0.3 percent of flavor composition. The preparation method disclosed by the invention comprises the following steps of: uniformly mixing the sweetening agent, the hydrophilic colloid and the preservative, then adding the mixer in water and uniformly stirring; heating the mixer until the hydrophilic colloid is completely dissolved; reducing the temperature to be 75-85DEG C; adding microalgae DHA powder, the antioxidant and the acidity regulator; adding to a certain weight with water; filling, sealing, sterilizing, fishing, leaching and cooling the mixer; and after the mixer is qualified by the inspection, obtaining the nutrient jelly containing the microalgae DHA. The nutrient jelly obtained by improving the formula and the preparation process retains original mouthfeeling within the guarantee period without adverse off flavor; and the microalgae DHA has favorable stability within the guarantee period of the jelly without influencing the mouthfeeling and the flavor of the jelly.
Description
Technical field
The invention belongs to the technical field of making food, relate to a kind of nutrition jelly that contains the little algae DHA of polyunsaturated fatty acid and preparation method thereof.
Background technology
Jelly is a kind of leisure food, also is a kind of healthy food of low heat energy high dietary-fiber, is raw material with edible hydrophilic colloid, white granulated sugar, fruit juice etc. mainly, through boiling the jelly food that working procedure processing such as glue, allotment, canned, sterilization form.Because of outward appearance sparkling and crystal-clear, lovely luster, sweet mouthfeel is smooth, receives particularly liking of youngsters and children friends who of consumers in general deeply.In recent years; The Along with people's living standard improve constantly and to the demand of food value; More pay attention to the nutritive value and the health care of jelly, jelly production also tends to nutrient laden, naturalization and functionalization further, and nourishing healthy jelly on the market is like rain the back spring bamboo; Become the new lover of food products market, for example vitamin E jelly, collagen jelly, vitamin D+calcium jelly etc.But the jelly production that contains polyunsaturated fatty acid is then very rare, therefore, develops little algae DHA jelly production of some pufa-containings more, enriches market jelly production kind and people's demand, seems beneficial.
DHA (Docosahexaenoic acid), DHA is commonly called as brain gold, is a kind of to the very important polyunsaturated fatty acid of human body, belongs to the important member in the Omega-3 polyunsaturated fatty acid family.DHA is a kind of essential element that nervous system cell is grown and kept; Be brain and amphiblestroid important composition composition; Content is up to 20% in the human brain cortex, and proportion is maximum in eye retina, accounts for 50%; DHA helps the eyesight of youngsters and children and eye to grow, the main performance in the following areas:
(1) promotes brain to grow, improve memory
DHA is one of main component of human brain, accounts for about 10% of people's cephalopin.DHA can promote division, propagation, the growth of projection and the formation of neutral net of brain cell, thereby cranial capacity is increased, and the brain internal information is handled and speeded, and helps the raising of intelligence, learning and memory ability.
(2) physiogeny of promotion eyesight
Big quantity research shows that DHA can promote eyesight physiogeny, if lack DHA, amphiblestroid DHA content reduces, can be influential to the visual sensitivity of light, and eyesight can reduce.This can promote the extension of optic nerve film and keep normal function relevant mainly due to DHA.DHA content in people's retina is abundant; DHA content is up to 18～22% of total fat mass in the amphiblestroid phosphatide of human body; Particularly content is very high in budding retina, accounts for 50% of whole aliphatic acid, and visible DHA is crucial to amphiblestroid formation.
Additional DHA mainly contains two approach, and the one, by synthetic in the body, the 2nd, through extraneous food intake.But research shows that the efficient that self transforms DHA in the human body is low-down, less than 0.5%, so can only replenish DHA through the approach of extraneous food intake.Because the influencing the content that human body absorbs every day can't reach the intake that international nutritive issue is advised far away from food of dietary structure; Special in the children that are in the growth and development stage stage, teenager; Replenish the normal development that a certain amount of DHA helps its heart and brain intelligence and eyesight every day, still, because mouthfeel and the taste of DHA itself are not good; Have slight marine alga and grow smell, directly take difficult by children, teenager's acceptance.Therefore, add DHA in the middle of the food that children, teenager like to, children, teenager replenish very effective approach of DHA beyond doubt.Abroad, DHA has been widely used in the various food, like baby formula milk powder, edible oil and fat, bread, biscuit, fruit drink, sour milk, candy, cheese etc.At home, improving constantly of Along with people's living standard more and more paid close attention to health, and the food that adds DHA more and more receives consumer's warm welcome.And one of food that jelly is liked as children, teenager the most; In jelly, add polyunsaturated fatty acid DHA; Not only can give jelly abundant nutritive value and health care, improve the product specification of jelly, and help children to replenish DHA; Promote the growth of children's mentality and eyesight, meet very much modern father and mother's the values and the needs of social long term growth.
People get used to being used as through feed ocean abyssal fishes or by the fish oil that deep-sea, ocean oils is refined the important source of additional DHA traditionally.Because numerous restrictions of feed ocean abyssal fishes (cost an arm and a leg, DHA content is low, the accumulating expense is high), generally all be to satisfy the needs of human body to DHA through taking in deep sea fish oil; But the EPA in the deep sea fish oil (eicosapentaenoic acid) is difficult to both are thoroughly separated with the DHA coexistence, therefore in fish oil, exists the EPA of high-load.EPA is the competitive inhibitor of AA (arachidonic acid); When EPA concentration in vivo is higher (improper physiological concentration); Its preferential cell phosphatide route of synthesis that gets into, the phosphatide that suppresses to be begun by AA synthesizes, thereby reduces the synthetic chance that contains eicosane class (like the prostaglandin) material of AA; This phenomenon has influenced growing of infant to neonatal growth tool retardation.And little algae DHA compares with deep sea fish oil; EPA (eicosapentaenoic acid) content is low, and structure is a triglyceride type, and does not have the special fishlike smell of marine pollutant (heavy metal, chemical insecticide, dioxin etc.) and fish; Thereby it is purer, safe, effective, and more welcome.At present, be badly in need of developing out the jelly production that more contains little algae DHA on the market, for children, the additional DHA of teenager provide a new approach.
Summary of the invention
Primary and foremost purpose of the present invention is to overcome the shortcoming and the deficiency of the existing little algae DHA of polyunsaturated fatty acid (DHA) application technology in the jelly system; A kind of anti-oxidation protection scheme to the little algae DHA of high-load is provided; Make it good, thereby produce the colory nutrition jelly production that contains little algae DHA at jelly shelf-life internal stability.
Another goal of the invention of the present invention is to provide the said preparation method who contains little algae DHA nutrition jelly.
The object of the invention is realized through following technical proposals: a kind of nutrition jelly that contains little algae DHA comprises following component by mass percentage:
Described little algae DHA can come from little algae DHA pulvis, the docosahexaenoic acid grease of little algae DHA pulvis for being made by the fermentation of little phycomycete kind, the little algae DHA of the microcapsules pulvis of processing through technology embedding such as emulsification, homogeneous, spray-drying; Its DHA content >=7.0%, embedding rate are more than 99.0%, and surface oil is below 0.50%; The source green safety; Do not contain marine pollutant (like Polychlorinated biphenyls, dioxin, furans etc.), outward appearance is white or buff powder, uniform particles; Free from admixture, nothing caking can be scattered in the water equably;
Described hydrophilic colloid is served as reasons and followingly is equivalent to the said material that contains little algae DHA nutrition jelly mass percent meter and forms:
Refining carragheen 0.11～0.28%
Konjacmannan 0.08～0.20%
Xanthans 0.01～0.02%;
Described sweetener is sucrose or the mixture be made up of sucrose and glucose syrup, malt syrup; When the mixture formed by sucrose and glucose syrup, malt syrup; The addition of sucrose must not be lower than said 8.0% of the little algae DHA nutrition jelly quality that contains, and its soluble solid content must not be lower than said 10.0% of the little algae DHA nutrition jelly quality that contains;
Described anticorrisive agent is the mixture of potassium sorbate or potassium sorbate and sorbic acid formation;
Described acidity regulator is by being equivalent to said 0.05～0.10% chelating salt that contains quality in little algae DHA nutrition jelly and being equivalent to said quality 0.20～0.40% organic acid that contains in little algae DHA nutrition jelly and forming;
Described chelating salt is preferably KCl;
Described organic acid is preferably citric acid, or the mixture that forms of at least a and citric acid in the lactic acid, malic acid, tartaric acid;
Said anti-oxidant be in L-ascorbyl palmitate, vitamin E, the Rosmarinus officinalis extract a kind of, two or three combine;
Said anti-oxidant more preferably L-ascorbyl palmitate, vitamin E and Rosmarinus officinalis extract is pressed 20: 5: 2 proportionings of mass ratio;
Described flavoring essence is preferably at least a in milk flavour, fragrant citrus essence, honey peach essence, mango essence, corn essence, strawberry essence, flavoring apple essence or the guava essence;
Food ingredient used in the present invention is food-grade;
Water used in the present invention all refers to meet running water or the pure water that country's " drinking water sanitary standard " (GB5749-2006) stipulates;
Packaging material used in the present invention also are food-grade, and heat sealability is good, and air-tightness is good.
The said preparation method who contains the nutrition jelly of little algae DHA comprises the steps:
(1) dry mixing becomes uniform mixture with sweetener with hydrophilic colloid, anticorrisive agent, and mixing soluble in water then leaves standstill 30～60min, under the state that stirs, is heated to 95～100 ℃, is incubated after hydrophilic colloid dissolves fully and obtains the jelly glue;
(2) with the warm water dissolving of little algae DHA and anti-oxidant, process little algae DHA emulsion with 45～55 ℃;
(3) treat that the jelly glue that step (1) obtains is cooled to 75～85 ℃, add little algae DHA emulsion that step (2) obtains, stir, adding the acidity regulator adjust pH again is 3.7～4.0; Add the flavoring essence blending, water is heavy surely then, obtains jelly solution;
(4) jelly solution is carried out can, seals;
(5) packaged jelly solution is incubated 12～15min in 85～95 ℃ water and carries out sterilization;
(6) pull out, drain, cooling processing after the assay was approved, obtains containing the nutrition jelly of little algae DHA;
Wherein, used amount of substance is following by mass percentage:
Little algae DHA0.002～0.0105%, hydrophilic colloid 0.20～0.50%, sweetener 10.0～15.0%, acidity regulator 0.25～0.50%, anticorrisive agent 0.02～0.05%, anti-oxidant 0.005～0.012%, flavoring essence 0.1～0.3%.
It is 20～25 ℃ water that water described in the step (1) is preferably temperature;
The time of the insulation described in the step (1) is preferably 5～10min;
The concrete steps of the cooling processing described in the step (6) are pulled out, are drained after being preferably the water cooling that places 20～25 ℃.
The present invention has following advantage and effect with respect to prior art:
(1) the used DHA of the present invention derives from the marine microalgae strain fermentation and makes; Compare with fish oil; Have following advantage: the firstth, the development sustainability is strong; Only need just can make little algae DHA continuously, can not consume a day exhausted to the greatest extent marine fishes resource through in the fermentation tank of factory, fermenting; Second owing to little algae is cultivated through biofermentation; Whole fermentation process is totally-enclosed; Have no pollution, therefore little algae DHA does not contain marine pollutant (like heavy metal Hg, Polychlorinated biphenyls 、 bioxin, furans etc.), is natural, safe, high-quality vegetalitas DHA; The 3rd EPA content is few, DHA: EPA in the little algae DHA pulvis that makes＞10: 1, and both proportionings are suitable, meet the requirement of the WHO/FAO of linked groups; The marine microalgae peculiar taste that having ideals, morality, culture, and discipline is slight has strong fishlike smell unlike fish oil, and normal children, teenager are prone to accept.
(2) well-known, DHA is a kind of polyunsaturated fatty acid, is prone to oxidation; Very responsive to light, heat, oxygen etc.; And oxidation rate aggravation under acid water system condition, in case oxidized, oxidation product is volatile materials such as lower ketones, aldehyde; These materials can make the food that adds DHA bad smells such as similar fishlike smell, paint-like flavour occur, are difficult to swallow.The food great majority that add DHA on the market are the food liquid of neutrality for solid food or pH; DHA character in suchlike product is more stable; And the pH value of jelly is acid among the present invention; And in the process of processing, need through a series of high-temperature process, for example infusion, sterilization etc., high temperature, low pH value are more prone to promote the oxidation of little algae DHA.Combination and the jelly prescription of the present invention through anti-oxidant, preparation technology's improvement have successfully solved the DHA stability problem of jelly in the shelf-life that contains little algae DHA.The present invention has confirmed the maximum addition of little algae DHA in jelly through a large amount of experiments, guarantees that little algae DHA can not produce bigger influence to the outward appearance and the mouthfeel of jelly in this addition scope; Through preferred anti-oxidant L-ascorbyl palmitate, vitamin E, Rosmarinus officinalis extract, anti-oxidation protection is provided in jelly for little algae DHA uses; Add little algae DHA pulvis emulsion when being cooled to 75～85 ℃ through employing fruit juice gel liquid; Avoided the process of high temperature infusion; Better protection DHA is not oxidized, makes the described nutrition jelly mouthfeel that contains little algae DHA in the shelf-life, keep original mouthfeel, smell can not occur.Show according to this DHA content detection result who contains little algae DHA jelly in addition: DHA remains unchanged at the shelf-life of jelly intensive amount basically, so the present invention provides novelty and detailed science data and foundation for the application of little algae DHA in jelly.
(3) interpolation of little algae DHA can provide abundant nutrition to consumers in general; Not only can promote the growth of children, teen-age intelligence and eyesight; And a kind of nutrition jelly that reducing blood lipid, hypotensive, effect such as delay senility are arranged is provided to the elderly consumer; Enriched the product category and the market demand, its economic benefit and social benefit are with considerable.
Description of drawings
Fig. 1 is preparation method's flow chart of the present invention.
The specific embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
Preparation contains the nutrition jelly of little algae DHA, and is as shown in Figure 1:
(1) 100g white granulated sugar, 2g hydrophilic colloid (wherein refining carragheen is 1.1g, and konjacmannan is 0.8g, and xanthans is 0.1g) and 0.2g potassium sorbate is even; Add the 750g temperature again and be 20～25 ℃ water; Stir, leave standstill 30min, be heated to 95 ℃ while stirring; Insulation 5min obtains the jelly glue.
(2) take by weighing 2.5g acidity regulator (wherein chelating salt KCl is 0.5g, and citric acid is 2g) with a small amount of warm water (45～55 ℃) dissolving, obtain acid solution.
(3) (DHA content is mass percent 7% to take by weighing the little algae DHA of 0.3g pulvis; Following examples are together) (L-ascorbyl palmitate, vitamin E and Rosmarinus officinalis extract are pressed 20: 5: 2 proportionings of mass ratio with the 0.05g compound antioxidant; Following examples are together) evenly, dissolve with a small amount of warm water (45～55 ℃).
When (4) treating that the jelly glue is reduced to 75 ℃ naturally, the acid solution that adding step (2) obtains, solution and the 1g fragrant citrus essence that step (3) obtains, and stir, the pH value is 3.7～4, and is heavy surely to 1000g with hot boiling water, obtains jelly solution.
(5) with jelly solution by quantitatively carrying out can, sealing.
(6) packaged jelly solution is incubated 12min in 85 ℃ of water and carries out sterilization.
(7) pull out, drain, placing temperature then is that 20～25 ℃ water cools off, and pulls out again, drains, and detects according to GB19883-2005, qualified after, obtain containing the nutrition jelly of little algae DHA.
Embodiment 2
(1) 120g white granulated sugar, 3.5g hydrophilic colloid (wherein refining carragheen is 2g, and konjacmannan is 1.35g, and xanthans is 0.15g) and 0.35g potassium sorbate are mixed; Add the 750g temperature again and be 20～25 ℃ water; Stir, leave standstill 30min, be heated to 95 ℃ while stirring; Insulation 5min obtains the jelly glue.
(2) take by weighing 3g acidity regulator (wherein chelating salt KCl is 0.8g, and citric acid is 2.2g),, obtain acid solution with a small amount of warm water (45～55 ℃) dissolving.
(3) take by weighing 1g little algae DHA pulvis and 0.08g compound antioxidant and mix, dissolve with a small amount of warm water (45～55 ℃).
When (4) treating that the jelly glue is reduced to 75 ℃ naturally, the acid solution that step (2) is obtained, the solution that step (3) obtains and 2g fragrant citrus essence add, and stir, and pH value 3.7～4 weighs to 1000g with hot boiling water surely, obtains jelly solution.
(5) with jelly solution by quantitatively carrying out can, sealing.
(6) packaged jelly solution is incubated 12min in 85 ℃ of water and carries out sterilization.
(7) pull out, drain, placing temperature then is that 20～25 ℃ water cools off, and pulls out again, drains, and detects according to GB19883-2005, qualified after, obtain containing the nutrition jelly of little algae DHA.
Embodiment 3
(1) 150g white granulated sugar, 5g hydrophilic colloid (wherein refining carragheen is 2.8g, and konjacmannan is 2g, and xanthans is 0.2g) and 0.5g potassium sorbate are mixed; Add the 750g temperature again and be 20～25 ℃ water; Stir, leave standstill 30min, be heated to 95 ℃ while stirring; Insulation 5min obtains the jelly glue.
(2) take by weighing 5g acidity regulator (wherein chelating salt KCl is 1g, and citric acid is 4g),, obtain acid solution with a small amount of warm water (45～55 ℃) dissolving.
(3) take by weighing 1.5g little algae DHA pulvis and 0.12g compound antioxidant and mix, dissolve with a small amount of warm water (45～55 ℃).
When (4) treating that the jelly glue is reduced to 75 ℃ naturally, the acid solution that step (2) is obtained, the solution that step (3) obtains and 3g fragrant citrus essence add, and stir, and pH value 3.7～4 weighs to 1000g with hot boiling water surely, obtains jelly solution.
(5) with jelly solution by quantitatively carrying out can, sealing.
(6) packaged jelly solution is incubated 12min in 85 ℃ of water and carries out sterilization.
(7) pull out, drain, placing temperature then is that 20～25 ℃ water cools off, and pulls out again, drains, and detects according to GB19883-2005, qualified after, obtain containing the nutrition jelly of little algae DHA.
Effect embodiment 1
Place normal temperature (20～25 ℃) and 42 ℃ of baking ovens to store respectively each product that obtains among the embodiment 1～3; The jelly of normal temperature storage carries out subjective appreciation to it every other month; The jelly that stores in 42 ℃ of baking ovens is every to carry out subjective appreciation at a distance from two weeks to it; And the product that obtains among the embodiment 3 carried out the detection of DHA content, follow-on test 3 months (42 ℃ of condition held were never degenerated and were equivalent to normal temperature under storage 1 year in 3 months).
(1) taste tests is carried out as follows:
Form sense organ evaluation and test group's (forming), smell, mouthfeel that each product of obtaining among the embodiment 1～3 stores in room temperature, 42 ℃ of baking ovens are evaluated and tested, according to the algae flavor being arranged and not having the algae two kinds of ranks of distinguishing the flavor of and evaluate by 6 people.Each the product sensory evaluation test result that obtains among the embodiment 1～3 is as shown in table 1
The subjective appreciation result of the little algae DHA of table 1 jelly under normal temperature and 42 ℃ of conditions
Annotate: jelly stores 1 day under 42 ℃ of conditions, is equivalent to store 4 days under the normal temperature, in other words, under 42 ℃ of conditions, stores 3 months, is equivalent to store 1 year under the normal temperature.
(2) little algae DHA content detection is carried out as follows:
The assay method of the DHA that employing is described below in jelly carries out the DHA content detection to the product that obtains among the embodiment 3, and each sample is done 2～3 parallel appearance, and final result representes that with mean value the result is as shown in table 2.
The assay method of DHA in jelly:
1. sampling: after the upper, middle and lower of jelly layer is taken a sample mixing respectively, take by weighing required sample size again.
2. pre-treating method: the title heavily about 2g of sample (accurately to 0.0001g) adds the hydrochloric acid solution that 9mL hot water (60 ℃～70 ℃) fully mixes back adding 1mL percent by volume 20% in test tube, and 60 ℃ of water-bath 30min also constantly stir.Add the ammoniacal liquor 1mL of mass percent 25%, 60 ℃ of water-bath 15min are cooled to room temperature; Add absolute ethyl alcohol, ether and benzinum (preparation in 2: 1: 1 by volume) intermixture 30mL and fully stir (or fully vibration), and under the 4000r/min condition centrifugal 3min or static layering, upper organic phase is incorporated in the beaker; Water repeats aforesaid operations lixiviate, centrifugal with 5mL ether and 5mL benzinum mixed liquor again; In upper organic phase merging and the beaker, water-bath evaporated under reduced pressure or nitrogen dry up to remove and desolvate, after solvent fully eliminates; Residual fat is pressed the dilution constant volume to 10mL (V) with normal heptane; Pipette 2mL and place screw-cap test tube, add 4moL/L potassium hydroxide-methanol solution 0.5mL, the lid of screwing; Fully more than the vibration 1min, static 10min clarifies to the reactant liquor layering; If organic layer is muddy, can centrifugally make it clarification; Draw upper strata organic layer liquid sample introduction, sample size is 0.5 μ L～1 μ L.
3. the detection of DHA
The preparation of A, titer
Take by weighing the about 0.1g of DHA triglyceride standard items (being accurate to 0.0001g); Be settled to 50mL, process about 2mg/mL standard items storing solution, get the 5mL storing solution again and be settled to 50mL; Process about 0.2mg/mL and use liquid; Below " pipette 2mL and place screw-cap test tube ... can centrifugally make it clarification " operation, draw upper strata organic layer liquid, be DHA (DHA) titer by 2. (in the pre-treating method).
B, system suitability condition
Capillary column DB-23 (30m * 0.25mm * 0.25 μ m), 250 ℃ of sample introduction temperature, 250 ℃ of detected temperatures; Column flow rate 2mL/min, initial temperature is 150 ℃, is warming up to 180 ℃ with 10.0 ℃/min; Be warming up to 220 ℃ of outlet temperatures with 6.0 ℃/min again, keep 6min.
C, quantitative assay
Inject a certain amount of titer and get into gas chromatograph, obtain the peak area Ai of DHA; Inject isopyknic sample liquid to be measured and get into chromatograph, obtain the peak area Bi of DHA in the sample
D, expression of results
In the formula: Bi---the corresponding peak area of component i (DHA) in the sample;
Csi---the mass concentration of component i in the titer, mg/mL;
V---the volume of testing sample dilution, mL;
Ai---the peak area of component i in the titer;
W---the product quality that takes by weighing, g.
The DHA testing result of the product of table 2 instance 3 under normal temperature and 42 ℃ of conditions
The result of table 1 and table 2 shows that jelly of the present invention does not have significant difference with general jelly on local flavor, have the jelly local flavor, no algae flavor; In appearance, transparency decreases, and can demonstrate milky, and the jelly state uniformity stores 90 days (being equivalent to 1 year shelf-life of normal temperature) under 42 ℃ of environment, and its DHA content does not have significant change.
The comparative example 1
Sample is made according to the prescription of embodiment 3, and just anti-oxidant kind and addition are different, and be specifically as shown in table 3.After sample making finishes, place 42 ℃ of baking ovens to store, every other month product is carried out the detection of DHA content, testing result is as shown in table 3:
Table 3
Can find out by table 3; The effect of compound antioxidant (L-ascorbyl palmitate, vitamin E and Rosmarinus officinalis extract are pressed 20: 5: 2 proportionings of mass ratio) is better than the effect of single anti-oxidant; Reason is that Rosmarinus officinalis extract can be contributed hydrogen atom and make vitamin E regeneration; Synergy between L-ascorbyl palmitate and vitamin E is; Vitamin E at first generates the vitamin E free radical with radical reaction, and vitamin E can be born again in the presence of the L-ascorbyl palmitate, generates L-ascorbyl palmitate free radical simultaneously up to the full consumption of L-ascorbyl palmitate.
The comparative example 2
Sample makes according to embodiment 3 steps, is after the addition of little algae DHA pulvis is adjusted into 2g (being the 14mg/100g finished product) sample making and finishes, to place 42 ℃ of baking ovens to store, and whenever at a distance from 15 days product is carried out subjective appreciation, and the result is as shown in table 4:
Table 4
When little algae DHA addition was 14mg/100g, 42 ℃ of condition held had the algae flavor to occur after 45 days slightly; Experiment continued to track to 60 days; Find that the algae flavor increases the weight of, therefore experiment is no longer followed the tracks of, and explains that the addition increase meeting of little algae DHA exerts an influence to the local flavor of jelly in the shelf-life.
The comparative example 3
Sample makes according to the following steps:
(1) 150g white granulated sugar, 5g hydrophilic colloid (wherein refining carragheen is 2.8g, and konjacmannan is 2g, and xanthans is 0.2g), 0.5g potassium sorbate, the little algae DHA of 1.5g pulvis, 0.12g compound antioxidant are mixed; Add the 750g temperature again and be 20～25 ℃ water; Stir, leave standstill 30min, be heated to 95 ℃ while stirring; Insulation 5min obtains the jelly glue.
(2) take by weighing 5g acidity regulator (wherein chelating salt KCl is 1g, and citric acid is 4g),, obtain acid solution with a small amount of warm water (45～55 ℃) dissolving.
When (3) treating that the jelly glue is reduced to 75 ℃ naturally, acid solution and 3g fragrant citrus essence that step (2) is obtained add, and stir, and pH value 3.7～4 is heavy surely to 1000g with hot boiling water, obtains jelly solution.
(4) with jelly solution by quantitatively carrying out can, sealing.
(6) packaged jelly solution is incubated 12min in 85 ℃ of water and carries out sterilization.
(7) pull out, drain, placing temperature then is that 20～25 ℃ water cools off, and pulls out again, drains, and detects according to GB19883-2005, qualified after, obtain containing the nutrition jelly of little algae DHA.
After sample preparation finishes, place 42 ℃ of baking ovens to store, whenever at a distance from 15 days product is carried out the detection of subjective appreciation and DHA content, the result is shown in table 5, table 6.
Table 5
Table 6
In comparative example 3, the adding technology of little algae DHA has been made adjustment, materials such as little algae DHA pulvis elder generation and hydrophilic colloid are done and are mixed; Different with original technology is that the process that the little algae DHA of adjusted technology need pass through the high temperature infusion can be known from table 5, table 6; The algae flavor has appearred in the jelly production that adopts adjusted technology to make at once, in 42 ℃ of baking ovens, store 15d, 30d after algae flavor still exist, and the jelly production DHA content loss rate that harsh output is come is up to more than 50%; DHA content does not have significant change after storing 30d; DHA is described owing to be sensitivity, so in the interpolation process, should avoids the high temperature infusion to cause damage thermoae.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. a nutrition jelly that contains little algae DHA is characterized in that containing little algae DHA.
2. the nutrition jelly that contains little algae DHA according to claim 1 is characterized in that comprising following composition by mass percentage:
3. the nutrition jelly that contains little algae DHA according to claim 2; It is characterized in that: described hydrophilic colloid is served as reasons and followingly is equivalent to the said material that contains little algae DHA nutrition jelly mass percent meter and forms: refining carragheen 0.11～0.28%; Konjacmannan 0.08～0.20%, xanthans 0.01～0.02%.
4. the nutrition jelly that contains little algae DHA according to claim 2 is characterized in that: described acidity regulator is by being equivalent to said 0.05～0.10% chelating salt that contains quality in little algae DHA nutrition jelly and being equivalent to said quality 0.20～0.40% organic acid that contains in little algae DHA nutrition jelly and forming.
5. the nutrition jelly that contains little algae DHA according to claim 4 is characterized in that: described chelating salt is KCl;
Described organic acid is a citric acid, or the mixture that forms of at least a and citric acid in the lactic acid, malic acid, tartaric acid.
6. the nutrition jelly that contains little algae DHA according to claim 2 is characterized in that: described anti-oxidant is the mixture of a kind of or at least two kinds of compositions in L-ascorbyl palmitate, delta-tocopherol or the Rosmarinus officinalis extract.
7. the nutrition jelly that contains little algae DHA according to claim 6 is characterized in that: described anti-oxidant is that L-ascorbyl palmitate, vitamin E and Rosmarinus officinalis extract are by 20: 5: 2 proportionings of mass ratio.
8. the nutrition jelly that contains little algae DHA according to claim 2 is characterized in that: described flavoring essence is at least a in milk flavour, fragrant citrus essence, honey peach essence, mango essence, corn essence, strawberry essence, flavoring apple essence or the guava essence.
9. the described preparation method who contains the nutrition jelly of little algae DHA of claim 2 is characterized in that comprising following steps:
(1) dry mixing becomes uniform mixture with sweetener with hydrophilic colloid, anticorrisive agent, and mixing soluble in water then leaves standstill 30～60min, under the state that stirs, is heated to 95～100 ℃, is incubated after hydrophilic colloid dissolves fully and obtains the jelly glue;
(2) with the warm water dissolving of little algae DHA pulvis and anti-oxidant, process little algae DHA emulsion with 45～55 ℃;
(3) treat that the jelly glue that step (1) obtains is cooled to 75～85 ℃, add little algae DHA emulsion that step (2) obtains, stir, adding the acidity regulator adjust pH again is 3.7～4.0; Add the flavoring essence blending, water is heavy surely then, obtains jelly solution;
(4) jelly solution is carried out can, seals;
(5) packaged jelly solution is incubated 12～15min in 85～95 ℃ water and carries out sterilization;
(6) pull out, drain, cooling processing after the assay was approved, obtains containing the nutrition jelly of little algae DHA;
Wherein, used amount of substance is following by mass percentage:
Little algae DHA0.002～0.0105%, hydrophilic colloid 0.20～0.50%, sweetener 10.0～15.0%, acidity regulator 0.25～0.50%, anticorrisive agent 0.02～0.05%, anti-oxidant 0.005～0.012%, flavoring essence 0.1～0.3%.
10. the preparation method who contains the nutrition jelly of little algae DHA according to claim 9 is characterized in that: the water described in the step (1) is that temperature is 20～25 ℃ a water;
The time of the insulation described in the step (1) is 5～10min;
The concrete steps of the cooling processing described in the step (6) are to pull out, drain after placing 20～25 ℃ water to cool off.
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| CN104642839A (en) * | 2015-02-06 | 2015-05-27 | 中国海洋大学 | Health fish oil jelly and preparation method thereof |
| CN105146238A (en) * | 2015-08-03 | 2015-12-16 | 广东省食品工业研究所 | Jelly capable of improving memory and preparation method of jelly |
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| CN104012825A (en) * | 2014-06-19 | 2014-09-03 | 南京麦思德餐饮管理有限公司 | Durian jelly and preparation method thereof |
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| CN104642839A (en) * | 2015-02-06 | 2015-05-27 | 中国海洋大学 | Health fish oil jelly and preparation method thereof |
| CN104642839B (en) * | 2015-02-06 | 2017-12-29 | 中国海洋大学 | A kind of health-care fish oil jelly and preparation method thereof |
| CN105146238A (en) * | 2015-08-03 | 2015-12-16 | 广东省食品工业研究所 | Jelly capable of improving memory and preparation method of jelly |
| CN105146238B (en) * | 2015-08-03 | 2019-01-01 | 广东省食品工业研究所有限公司 | A kind of jelly and preparation method thereof with improvement memory |
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| CN102423021B (en) | 2013-04-03 |
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Address after: 515041 Guangdong province Shantou Changping Road 90, Suning Plaza Building 2 (8-23), 2106, 2107, 2105, 2108, 2109. Patentee after: Guangdong branch of biological engineering Limited by Share Ltd Address before: 515041 Guangdong Province, Shantou city Longhu District East Road Longsheng industrial zone Patentee before: Guangdong Runke Bioengineering Co., Ltd. |