CN102318633A - Sterilization composition - Google Patents
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- CN102318633A CN102318633A CN201110188628A CN201110188628A CN102318633A CN 102318633 A CN102318633 A CN 102318633A CN 201110188628 A CN201110188628 A CN 201110188628A CN 201110188628 A CN201110188628 A CN 201110188628A CN 102318633 A CN102318633 A CN 102318633A
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Abstract
The invention relates to a sterilization composition in the farm chemicals compounded field, which is characterized in that: active component bixafen is mixed with different dicarboximide compound sterilization agents, the weight percentage of the two active components is 80:1 to 1:90, and the preferential weight percentage is 50:3 to 5:70; and the total weight percentage of the active components in the sterilization composition is 1 percent to 90 percent, the preferential total weight percentage of the active components is 5 percent to 75 percent, and the rest of components in the sterilization composition are auxiliary components which are allowed to use and are acceptable in the farm chemicals. The sterilization composition can be made into wettable powder, suspending agent, fumicants, solubable liquid, emulsion in water and water dispersion granule through a known method.
Description
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of bixafen of containing (C
18H
12Cl
2F
3N
3O) and several kinds of dicarboximide compounds iprodiones, dimethachlon, procymidone, chlorobenzenes cough up and be two yuan of composite bactericidal compositions of active ingredient one of arbitrarily in bacterium amine, the vinclozolin.
Background technology
Bixafen (C
18H
12Cl
2F
3N
3O), be a kind of novel SDI pyrazoles bactericide, the chemical structural formula of bixafen is:
Bixafen is the succinate dehydrogenase inhibitor; With mainly developing the control that is used for leaf spot and leaf rust; But show through research; This new compound all has excellent activity to multiple germ, like the comparatively general germ of pesticide resistance such as white powder germ, ash arrhizus bacteria, Pseudoperonospora cubensis, is expected to become the important kind of bactericide resistance management.
Iprodione is that the dicarboximide class is efficient, wide spectrum, contact killing type bactericide, has certain therapeutic action simultaneously, also can absorb systemic action through root.Through the CKIs kinases, control the interior signal of cell of many cell functions, comprise that carbohydrate combines to get into the interference effect of fungal cell's component.Therefore, it can suppress the sprouting and the generation of fungal spore, also can suppress mycelial growth.Be applicable to various vegetables such as melon, tomato, capsicum, eggplant, landscape flower, lawn and ornamental plants etc.The disease of main controlling object for causing by botrytis, corn marigold, Alternariaspp, sclerotinite etc.Like gray mold, early blight, black spot, stalk break etc.Can effectively prevent and treat the fungi that the benzimidazole systemic fungicide is had resistance.
Dimethachlon has characteristics such as wide spectrum, sterilization, interior suction, infiltration, treatment, lasting period length.Inhale the conduction double action in having, foliage-spray can be transmitted to the disease position rapidly, thoroughly sterilization in reaching by table, and multiple germ is once removed.
Procymidone is interior absorption fungicide, has the double action of protection and treatment.Can suppress the synthetic of the interior triglycerides of thalline, Botrytis and Sclerotinia fungi are had special efficacy, can prevent and treat gray mold, the stalk break of fruit tree, vegetable crop, the fungi that benzimidazole is produced resistance is also effective.Protection is effective after using, the lasting period is long, can stop the scab developmenting spread.
Vinclozolin is a kind of protectiveness and tagging property bactericide of wide spectrum, and the disease that is caused by disease funguses such as Botrytis cinerea genus, Sclerotinia, chain sclerotinia sclerotiorum genus on the plants such as fruit trees such as grape, vegetables, ornamental plants is had significant prevention and therapeutic action.
Iprodione, dimethachlon, procymidone, chlorobenzene are coughed up bacterium amine, vinclozolin through using separately for a long time; Produced very big pesticide resistance; Preventive effect to fungal disease reduces greatly, and dosage strengthens, and the lasting period is not long; The repeated drug taking increased frequency is not unfavorable for the sustainable development of environment very much.But more than several kinds of bactericide, interior absorption is better, have concurrently simultaneously the protection and therapeutic action, cost performance is higher, the market space is bigger.But in order better to overcome the above problems, I research staff of company attempts with the new type bactericide of different effects mechanism composite with it, to improve its preventive effect; Can also reduce the manufacturing cost and use cost of new type bactericide,, in indoor prescription screening, chance on through much amount indoor virulence tests and field experiment; New type bactericide bixafen and iprodione, dimethachlon, procymidone, chlorobenzene cough up bacterium amine, two yuan of vinclozolin series bactericidal agents are composite; Synergistic effect is obvious, and preventive effect significantly improves, and dosage significantly reduces; Simultaneously also enlarge fungicidal spectrum, improved the actual efficiency of sterilization.And, do not see relevant report at present at home and abroad as yet about new type bactericide bixafen and the above-mentioned several kinds of researchs that the dicarboximide compounds is composite.
Summary of the invention
I research staff of company is devoted for years in the research in the composite field of agriculture chemicals fungicide; Through a large amount of formula test screenings; A kind of bactericidal composition of new high-efficiency has been proposed; Bactericidal composition of the present invention is to cough up one of any in bacterium amine, the vinclozolin with new type bactericide bixafen and several kinds of dicarboximide compounds iprodiones, dimethachlon, procymidone, chlorobenzenes to be that two yuan of carrying out of active ingredient are mixed, to be used to prevent and treat the diseases such as powdery mildew, rust, downy mildew, eqpidemic disease, leaf spot, anthracnose and rice blast of various crop, can obviously improve actual preventive effect; Delay the drug-fast generation of germ, reduce drug cost.
To cough up one of any in bacterium amine, the vinclozolin with new type bactericide bixafen and several kinds of dicarboximide compounds iprodiones, dimethachlon, procymidone, chlorobenzenes be that active ingredient is carried out two yuan of bactericidal composition preparations that are mixed for a kind of in described the present invention; To cough up percentage by weight one of any in bacterium amine, the vinclozolin be 80: 1～1: 90 for active ingredient new type bactericide bixafen and iprodione, dimethachlon, procymidone, chlorobenzene in the bactericidal composition wherein of the present invention; On the basis of the screening of test recipe targetedly, can active ingredient new type bactericide bixafen and iprodione, dimethachlon, procymidone, chlorobenzene be coughed up percentage by weight one of any in bacterium amine, the vinclozolin and be preferably 50: 3～5: 70.
Several kinds of dicarboximide compounds wherein of the present invention are that iprodione, dimethachlon, procymidone, chlorobenzene are coughed up in bacterium amine, the vinclozolin one of any.
The formulations of pesticide that pesticide sterilizing composite of the present invention uses the preparation method in pesticidal preparations field to be prepared into are wetting powder, suspending agent, fumicants, soluble liquid, aqueous emulsion and water dispersible granules; The gross mass of active ingredient accounts for 1%～90% of preparation gross mass in the preparation; Be preferably 5%～75%, all the other are usual auxiliaries in the agricultural chemicals.
Pesticide sterilizing composite of the present invention to grain, cotton, oil, really, the multiple fungal diseases of vegetables, flowers all has favorable effect, especially diseases such as leaf spot, powdery mildew, rust, downy mildew, eqpidemic disease, anthracnose and rice blast had excellent preventive effect.For example prevent and treat stinking smut, head blight; Rice blast, banded sclerotial blight; Sclerotinia rot of colza; Cotton disease; Sweet potato black rot; Cucurbits powdery mildew, anthracnose, gray mold; The Kidney bean gray mold; Powdery mildew of pea, brown spot; Floral crops diseases, downy mildew of garpe, wheat rust, melon anthracnose and downy mildew etc.
In addition, bactericidal composition of the present invention is compared similar medicament has a lot of other obvious advantages, and the first, it is more lasting to compare the single-dose effect, and quick-acting also has raising, and protection and therapeutic action are obvious.The second, delayed the drug-fast generation of crop, reduced the access times repeatedly of agricultural chemicals, reduced the agricultural chemicals usage amount, reduced agricultural cost; The 3rd, the residual quantity of this bactericidal composition is lower, and is friendly more to environment, and the environmental protection of agricultural region is significant.
Embodiment:
In order to make the object of the invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to these examples absolutely not.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is a mass ratio.Should be pointed out that all any modifications of within spirit of the present invention and principle, being made, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Wherein prepare required necessary basis component about the formulations of pesticide and the easy manufacture implementation method following:
Bixafen (C in following implementer's case
18H
12Cl
2F
3N
3O) represent with active principle A that it is one of any that on behalf of iprodione, dimethachlon, procymidone, chlorobenzene, active principle B cough up in bacterium amine, the vinclozolin.
One of technical scheme of the present invention, described bactericidal composition are wetting powder, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with active principle A and active principle B and dispersant, wetting agent and filler mixing; In stirred tank, evenly stir; Behind airslide disintegrating mill, mixing, promptly can be made into the wetting powder of the present composition.
Two of technical scheme of the present invention, described bactericidal composition are suspending agent, and the percentage by weight of component is:
The concrete production stage of this suspending agent is earlier other auxiliary agents to be mixed; Mix through high speed shear, add active principle A and active principle B, abrading-ball is 2～3 hours in ball crusher; Make a diameter all below 5mm, make the suspending agent preparation of bactericidal composition of the present invention.
Three of technical scheme of the present invention, described bactericidal composition are aqueous emulsion, and the percentage by weight of component is:
The concrete production stage of this aqueous emulsion is: at first former medicine active principle A is added with active principle B, solvent and emulsifier, cosolvent and be in the same place, make and be dissolved into uniform oil phase; With part water, antifreeze, other insecticides adjuvant such as antimicrobial mixes into uniform aqueous phase; When the agitated reactor high speed stirs, oil phase is added water, slowly add water, open clipper and carry out high speed shear, and add remaining water, shear half an hour approximately, form the aqueous emulsion of oil-in-water type until reaching the phase inversion point.Promptly make the aqueous emulsion of bactericidal composition of the present invention.
Four of technical scheme of the present invention, described bactericidal composition are water dispersible granules, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition water dispersible granules is: by above-mentioned prescription active principle A, active principle B and dispersant, wetting agent, disintegrant and filler are mixed; Pulverize with micro jet; Through mediating; Add then and carry out granulation, drying, screening in the fluidized bed prilling dryer after sample analysis, promptly make the water dispersible granules of bactericidal composition of the present invention.
Described emulsifier is selected from any one or more mixtures formed with arbitrary proportion in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, APES sulfosuccinate, styryl phenol APEO, NPE, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, the polyoxyethylene aliphatic alcohol ether.
Said solvent is any one or more mixed solvents formed with arbitrary proportion in xylol or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), the water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, APES, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, draw back in powder copper rosinate X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, the soapberry powder one or more.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, the resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, the glycerine.
Described antifoaming agent is selected from: silicone oil, silicone compound, C
10-20Saturated fat acid compounds, C
8-10In the aliphatic alcohols compound one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
Indoor virulence test and field experiment
The indoor virulence measuring:
Biologicall test instance 1:
(1) experimental subjects:
The cucumber downy mildew germ, separation and purification gained from the cucumber downy mildew incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Supply the examination medium:
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCl0.5g/L, KH
2PO
41.5g/L, agar powder 20g/L, deionized water 1L.
(2) experimental pharmacy:
The former medicine of 90%bixafen, Bayer crop science Co., Ltd, the former medicine of 96% iprodione, Huifeng Agricultural Chemistry Co., Ltd., Jiangsu.
Combination setting is mixed:
21%bixafen iprodione percentage composition was respectively 5: 16,7: 14,9: 12,11: 10,13: 8, i.e. 5%+16%, 7%+14%, 9%+12%, 11%+10%, 13%+8% totally 5 combinations that are mixed.
(3) test method:
Single agent is to the toxicity test of cucumber downy mildew germ:
Bixafen and iprodione dissolve with proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of following low temperature, and be for use, is diluted to a series of concentration with sterile water before the test.
The cucumber downy mildew germ after cultivating in advance on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, cultivates down in 25 ℃ of dark conditions.Every processing repetition 3 times.When control strain colony diameter length is above to 6.5cm, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single agent suppress mycelial growths
50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single agent bixafen and iprodione are carried out toxicity test, EC in order to last method
50Value is followed successively by 4.75mg/l and 13.67mg/l.
The mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ confession reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ confession reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is greater than 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action.
(4) result and analysis:
Table 1bixafen and iprodione are to the co-toxicity test of cucumber downy mildew germ
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| bixafen(A) | Y＝1.6752X+3.8664 | 0.9714 | 4.75 |
| Iprodione (B) | Y＝1.7428X+3.0205 | 0.9632 | 13.67 |
| A: B is 5: 16 | Y＝1.6933X+3.6403 | 0.9587 | 6.35 |
| A: B is 7: 14 | Y＝1.5981X+3.8529 | 0.9502 | 5.22 |
| A: B is 9: 12 | Y＝1.7524X+3.7840 | 0.9649 | 4.94 |
| A: B is 11: 10 | Y＝1.6455X+3.8827 | 0.9751 | 4.78 |
| A: B is 13: 8 | Y＝1.5281X+4.0041 | 0.9673 | 4.51 |
Table 2bixafen and iprodione are to the co-toxicity test of cucumber downy mildew germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Iprodione (B) | 0∶1 | 45.21 | 45.21 | / | / |
| A∶B | 5∶16 | 74.77 | 50.28 | 148.69 | Synergy |
| A∶B | 7∶14 | 90.97 | 56.50 | 161.02 | Synergy |
| A∶B | 9∶12 | 96.12 | 62.71 | 153.27 | Synergy |
| A∶B | 11∶10 | 99.48 | 68.93 | 144.32 | Synergy |
| A∶B | 13∶8 | 105.27 | 75.14 | 140.09 | Synergy |
Measuring the result according to above indoor virulence test shows; Bixafen and iprodione all have synergistic effect preferably by the percentage composition of experiment; Wherein with bixafen: iprodione is that 7: 14 o'clock co-toxicity coefficient is up to 161.02, thinks that bixafen and iprodione percentage composition are that 7: 14 o'clock synergistic effects are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 14 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test instance 2:bixafen and procymidone to the gray mold of cucumber germ.
The former medicine of 98.5% procymidone, Jiangsu Heyi Chemical Industry Co., Ltd..
The gray mold of cucumber germ, separation and purification gained from the gray mold of cucumber incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and procymidone are carried out toxicity test, EC
50Value is followed successively by 5.36mg/l and 11.59mg/l.
Combination setting is mixed:
23%bixafen procymidone percentage composition was respectively 5: 18,7: 16,9: 14,11: 12,13: 10, i.e. 5%+18%, 7%+16%, 9%+14%, 11%+12%, 13%+10% totally 5 combinations that are mixed.
Test method is with biologicall test embodiment 1
Experimental result and analysis:
Table 3bixafen and the composite virulence experiment of procymidone to the gray mold of cucumber germ
The composite co-toxicity test of table 4bixafen and procymidone to the gray mold of cucumber germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Procymidone (B) | 0∶1 | 45.60 | 45.60 | / | |
| A∶B | 5∶18 | 91.32 | 57.93 | 157.64 | Synergy |
| A∶B | 7∶16 | 105.35 | 62.61 | 168.27 | Synergy |
| A∶B | 9∶14 | 102.74 | 67.28 | 152.71 | Synergy |
| A∶B | 11∶12 | 106.05 | 71.95 | 147.38 | Synergy |
| A∶B | 13∶10 | 108.09 | 76.63 | 141.05 | Synergy |
Measuring the result according to above indoor virulence test shows; The used bixafen and the percentage composition of procymidone all have synergistic effect in the experiment; Wherein the co-toxicity coefficient when the percentage composition of bixafen and procymidone is 7: 16 is 168.27 to the maximum, thinks that promptly 7: 16 o'clock synergistic effects of percentage composition of bixafen and procymidone are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 16 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test instance 3:bixafen and dimethachlon to gray mold of cucumber.
The gray mold of cucumber germ, separation and purification gained from the cucumber canopy incidence of leaf in Shouguang, Shandong is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and dimethachlon are carried out toxicity test, EC
50Value is followed successively by 5.28mg/l and 13.44mg/l.
Combination setting is mixed:
The percentage composition of 20%bixafen dimethachlon was respectively 5: 15,7: 13,9: 11,11: 9,13: 7, i.e. 5%+15%, 7%+13%, 9%+11%, 11%+9%, 13%+7% totally 5 combinations that are mixed.
Test method is similar with biologicall test embodiment 1
Result and analysis:
Table 5bixafen and the composite co-toxicity of dimethachlon to the gray mold of cucumber germ
Table 6bixafen and the composite co-toxicity of dimethachlon to the gray mold of cucumber germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Pyrenomycetes clean (B) | 0∶1 | 43.83 | 43.83 | / | / |
| A∶B | 5∶15 | 77.89 | 54.46 | 143.02 | Synergy |
| A∶B | 7∶13 | 95.45 | 60.54 | 157.68 | Synergy |
| A∶B | 9∶11 | 98.13 | 66.61 | 147.33 | Synergy |
| A∶B | 11∶9 | 102.53 | 72.68 | 141.07 | Synergy |
| A∶B | 13∶7 | 106.83 | 78.75 | 135.66 | Synergy |
Mensuration result according to the test of bactericide co-toxicity; When bixafen and dimethachlon percentage composition are above experiment ratio; Result of the test shows all has synergistic effect; And the co-toxicity coefficient when ratio is 7: 13 is 157.68 to the maximum, and we can think that bixafen and the clean percentage composition of pyrenomycetes are at 7: 13 o'clock, and synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 13 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test instance 4:bixafen and vinclozolin to gray mold of cucumber.
The gray mold of cucumber germ, separation and purification gained from the cucumber canopy incidence of leaf in Shouguang, Shandong is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and vinclozolin are carried out toxicity test, EC
50Value is followed successively by 7.37mg/l and 15.69mg/l.
Combination setting is mixed:
The percentage composition of 25%bixafen vinclozolin was respectively 5: 20,7: 18,9: 16,11: 14,13: 12, i.e. 5%+20%, 7%+18%, 9%+16%, 11%+14%, 13%+12% totally 5 combinations that are mixed.
Test method is similar with biologicall test embodiment 1
Result and analysis:
Table 7bixafen and the composite co-toxicity of vinclozolin to the gray mold of cucumber germ
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| bixafen(A) | Y＝2.043X+3.2278 | 0.9421 | 7.37 |
| Vinclozolin (B) | Y＝2.0124X+2.5939 | 0.9536 | 15.69 |
| A: B is 5: 20 | Y＝2.3421X+2.7216 | 0.9612 | 9.39 |
| A: B is 7: 18 | Y＝2.0057X+3.1770 | 0.9508 | 8.11 |
| A: B is 9: 16 | Y＝2.4608X+2.7843 | 0.9641 | 7.95 |
| A: B is 11: 14 | Y＝2.3346X+2.9389 | 0.9409 | 7.64 |
| A: B is 13: 12 | Y＝2.2279X+3.0664 | 0.9516 | 7.38 |
Table 8bixafen and the composite co-toxicity of vinclozolin to the gray mold of cucumber germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Vinclozolin (B) | 0∶1 | 42.84 | 42.84 | / | / |
| A∶B | 5∶20 | 78.47 | 57.58 | 136.28 | Synergy |
| A∶B | 7∶18 | 90.91 | 61.82 | 147.05 | Synergy |
| A∶B | 9∶16 | 92.71 | 66.06 | 140.33 | Synergy |
| A∶B | 11∶14 | 96.52 | 70.30 | 137.29 | Synergy |
| A∶B | 13∶12 | 99.91 | 74.55 | 134.02 | Synergy |
Mensuration result according to the test of bactericide co-toxicity; When bixafen and vinclozolin percentage composition are above experiment ratio; Result of the test shows all has synergistic effect; And the co-toxicity coefficient when ratio is 7: 18 is 147.05 to the maximum, and we can think that the percentage composition of bixafen and vinclozolin is at 7: 18 o'clock, and synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 18 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Field trial 1: to the preventive effect of gray mold of cucumber
(1) investigation method
Experimental field be located at Shouguang, Shandong, publish the medium cucumber field of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every sub-district, look into 5 caves at every, and 2 blades in the fixing middle part of every strain fix a point to check during investigation.
Come classification according to the gray mold of cucumber grade scale, write down total strain number, diseased plant number, degree of being in a bad way, calculate diseased plant rate, disease index and preventive effect.The gray mold of cucumber grade scale is:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 9 is respectively organized the test effect that test medicine is handled the control gray mold of cucumber
Interpretation of result:
Can know that by table 9 effect of the combination control gray mold of cucumber that is mixed of 7%bixafen+14% iprodione very significantly.Under same 10 milliliters every mu consumption, the effect of complex preparation significantly is superior to two kinds of single agent, and complex preparation significantly is superior to the effect under the low concentration consumption in control efficiency under the consumption of high concentration.20 days preventive effects reach 90.33% after 10 days best control efficiency behind the 1st, 2 medicine reaches 83.55% and 87.63%, the 2 medicine respectively.And single agent 10%bixafen EC; 10 days the highest preventive effect is 81.18% behind the 2nd medicine; 20 days preventive effects are 71.44% behind the 2nd medicine of 50% iprodione WP; The effect of complex preparation significantly is superior to two kinds of single agent, has not only significantly improved the effect for the gray mold of cucumber germ, and has further prolonged its lasting period.
The experiment of comprehensive above indoor and outdoor is said; Coughing up one of any in bacterium amine, the vinclozolin with new type bactericide bixafen and several kinds of dicarboximide compounds iprodiones, dimethachlon, procymidone, chlorobenzenes is that active ingredient is carried out two yuan of bactericidal composition preparations that are mixed the diseases such as gray mold, powdery mildew, rust, downy mildew, eqpidemic disease, anthracnose and rice blast of various crop is all had the better prevention effect; Can effectively suppress further developing of disease; Simultaneously to crop safety; Suggestion is promoted the use of, and in use should be used alternatingly with other different mechanisms medicines, to delay the pesticide resistance of plant.
Claims (5)
1. pesticide sterilizing composite, it is characterized in that: this bactericidal composition is that (molecular formula is C with A novel pyrazole series bactericidal agent bixafen
18H
12Cl
2F
3N
3O) cough up in bacterium amine, the vinclozolin with B iprodione, dimethachlon, procymidone, chlorobenzene and be the preparation of two yuan of composite bactericidal compositions of active ingredient one of arbitrarily in one of any; All the other components are usual auxiliaries in the agricultural chemicals, and wherein the percentage by weight of effective constituents A and B is 80: 1～1: 90 in this bactericidal composition
A novel pyrazole series bactericidal agent bixafen.
2. bactericidal composition according to claim 1 is characterized in that: the percentage by weight of effective constituents A and B can further be preferably 50: 3～5: 70 in this bactericidal composition.
3. bactericidal composition according to claim 1 is characterized in that: to account for the percentage of gross weight in the preparation be 1%～90% for effective constituents A and B total content in this bactericidal composition.
4. bactericidal composition according to claim 3 is characterized in that: effective constituents A and B total content account for that the percentage of gross weight is preferably 5%～75% in the preparation in this bactericidal composition.
5. bactericidal composition according to claim 1 is characterized in that: the preparation formulation that bactericidal composition of the present invention can be mixed with is wetting powder, suspending agent, fumicants, soluble liquid, aqueous emulsion and water dispersible granules.
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| US20070060579A1 (en) * | 2003-10-10 | 2007-03-15 | Ulrike Wachendorff-Neumann | Synergistic fungicidal active substance combinations |
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| US20070060579A1 (en) * | 2003-10-10 | 2007-03-15 | Ulrike Wachendorff-Neumann | Synergistic fungicidal active substance combinations |
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Application publication date: 20120118 |