CN101948780B - Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof - Google Patents
Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Fertilizers (AREA)
Abstract
The invention relates to an antagonist bacterium which can overcome or eliminate a hot pepper epidemic disease and produced microbial organic fertilizer thereof, belonging to an intensive agriculture production technology. By the invention, an antagonist bacterium (Bacillus pumilus NJLZ-8) which has an obvious antagonist function on pathogenic oomycetes of the hot pepper continuous cropping epidemic disease is separated; the antagonist bacterium is mixed with organic fertilizer to prepare microbial organic fertilizer; and the fertilizer contains more than 1*108 antagonist microorganisms per gram, 4-5 percent of total nitrogen ( more than 90 percent of organic nitrogen), 6-10 percent of total nitrogen-phosphorus-potassium nutrients and 30-35 percent of organic matter. Proved by tests, the microbial organic fertilizer can rapidly breed antagonist bacteria after being applied into soil, form a preponderant group in the soil and has more than 80 percent of biological preventing and treating rate on the hot pepper epidemic disease in the soil with serious hot pepper epidemic disease. The generation of the hot pepper epidemic disease can be prevented to a large extent by applying the microbial organic fertilizer on the soil without forming continuous cropping obstacles for a long time.
Description
One, technical field
The present invention relates to a kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and treat the continuous cropping capsicum epidemic disease, belong to the intensive agriculture production technology, be exclusively used in and overcome and eliminate continuous cropping capsicum epidemic disease problem.
Two, background technology
Capsicum originates in the Central and South America torrid zone and subtropical zone, now in China's various places common cultivated, becomes one of maximum vegetable crop of China cultivated area, especially the important export advantage cash crop of earning foreign exchange.Along with the increase of the cultivation time limit, pepper planting district soil-borne disease takes place on a large scale, and particularly the appearance of management modes such as the industrialization of facility production, Focus, mass-producing makes soil-borne disease more become serious.Capsicum epidemic disease is caused by oomycetes pathogenic agent Phytophthora capsici, is the most serious soil-borne disease on capsicum produces, and generation was all arranged from the seedling stage to the fruiting period.Traditional chemical prevention and control method can cause negative impact to the environment and the mankind, if do not prevent and treat the effective ways and the approach of this disease, this will produce very big influence to the capsicum industry of China and the people's life.Our result of study shows that the key issue of succession crop eqpidemic disease is not nutrient deficiency or imbalance, neither the soil secondary salinization problem, but the intoxicating problem of micro-flora in the soil and secondary substance thereof.
On the other hand, the feces of livestock and poultry behind crop material on-site incineration, the large-scale cultivations such as paddy rice, wheat, corn, rape has been thrown aside etc. not only severe contamination everywhere environment, also greatly having wasted can be as the raw material of fertilizer and biological organic fertilizer product; A large amount of nutrient resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened the ability of China's agricultural sustainable development significantly.The nutritive element how will from soil, take away because of crop harvesting is returned in the soil to greatest extent and goes, and unique approach is processed the organic commercial fertilizer material with these solid organic castoffs exactly and is manured into soil.If through the synthetic high-grade organic compost of thermophilic fermentation, as the carrier of function yeast, made microorganism organic fertilizer definite functions will have good application prospects again with these solid organic castoffs.
Three, summary of the invention
1. technical problem
The object of the present invention is to provide a kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and treat the continuous cropping capsicum epidemic disease; The efficient of preventing and treating of continuous cropping capsicum epidemic disease is reached more than 80%; Make a large amount of industrialized agriculture soil and open country capsicum continuous cropping obstacle soil obtain biological prosthetic, guarantee agriculture the shaping up that intensify.
2. technical scheme
One strain can overcome or eliminate the antagonism bacterium NJLZ-8 of continuous cropping capsicum epidemic disease; This mikrobe belongs to the bacillus pumilus (Bacillus pumilus) of Gram-positive, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, address: Datun Road, Chaoyang District, Beijing City on July 9th, 2009; Institute of Microorganism, Academia Sinica; Culture presevation number is CGMCC NO.3182, and main biological characteristics is: main biological characteristics is Gram-positive (G+), produces gemma.Cell is shaft-like, aerobic or amphimicrobian growth, and catalase is positive, oxidase negative; The starch hydrolysis is negative, the V.P. reacting positive, and methyl red test is negative, and glucose fermentation produces not aerogenesis of acid; Unfermentable wood sugar, L-arabinose, N.F,USP MANNITOL can not utilize Citrate trianion, the nitrate reductase enzyme positive; Gelatin liquefaction positive, lecithinase is negative, not decomposition of cellulose.
The microorganism organic fertilizer of preventing and kill off the continuous cropping capsicum epidemic disease with above-mentioned antagonism bacterium produces contains 1 * 10 in this fertilizer
8The antagonism bacterium NJLZ-8 that individual/g is above, full nitrogen quality is that 4～5% (being organonitrogen 90% or more), total nitrogen phosphorus potassium nutrition are 6～10%, organic content is 30～35% than content.
The working method of said microorganism organic fertilizer comprises:
1) antagonism bacterium NJLZ-8 is inoculated into the PDA nutrient solution, carries out liquid fermenting production, the condition of fermentative prodn is: pH growth scope nature; 30～35 ℃ of culture temperature scopes; Stirring velocity is 180～300 rev/mins, and the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount>=1 * 10 in the fermented liquid
10Individual/ml.Used PDA nutrient solution compound method does, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling; Add the 20g common sucrose in the filtrating after filtering; Be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
2) with antagonism bacterium NJLZ-8 fermented liquid respectively with become thoroughly decomposed after pig manure and dish dregs of rice mikrobe enzymolysis mixture carry out solid fermentation; Add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton; Turning every day is 1 time in the solid fermentation process; Make the solid fermentation temperature be no more than 50 ℃, ferment and finish after 5 days, make antagonism bacterium NJLZ-8 content reach 1 * 10
8Individual/more than the g; Obtain pig manure microbial organic fertilizer and dish dregs of rice enzymolysis mixture microbial organic fertilizer, the pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content>=35%; The organonitrogen quality is 1.2-2% than content, water cut mass ratio 25-30%.The mikrobe enzymolysis preparation method of mixture of its Chinese food dregs of rice does, is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material with preserving number, regulates water cut 55-65% and pH 6.0-7.5; Carry out open solid fermentation, treat to begin immediately when leavening temperature rises to 50 ℃ turning, later turning every day; Make the heap temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation began, the pH of stacking yard can constantly rise; During each turning with containing acid solution body adjusting water cut and pH; Make them maintain 55-65% and 6.0-7.0 respectively, spray with containing the acid solution body again when fermentation stops, be adjusted into about 5.0 until stacking yard pH; Carry out cryodrying or natural airing again, be the mikrobe enzymolysis mixture of the dish dregs of rice.
3) then with the pig manure microbial organic fertilizer of solid fermentation and 60: 40 by volume ratio thorough mixing after-ripening of dish dregs of rice enzymolysis mixture microbial organic fertilizer 2 days; Turning is 2 times in the after-ripening process; With the water cut of after-ripening thing cryodrying to 30%, packing is dispatched from the factory and is the microbial organic fertilizer that can prevent and treat the continuous cropping capsicum epidemic disease.
Said microorganism organic fertilizer can be exclusively used in control continuous cropping capsicum epidemic disease.
3. beneficial effect
The present invention relates to a kind of microorganism organic fertilizer and working method thereof that can overcome or eliminate the continuous cropping capsicum epidemic disease; Utilize pig manure and dish dregs of rice mikrobe enzymolysis aminoacid mixture; Process microorganism organic fertilizer again with after antimicrobial fluid short of money is mixed, its product is compared with product in the market has following advantage:
1) contain the efficient bacterial strain of specificity (bacillus pumilus NJLZ-8) that suppresses the growth of capsicum epidemic disease cause of disease oomycetes in the fertilizer product, it suppresses the effect highly significant, and the content of this efficient bacterial strain reaches 1 * 10 in the fertilizer
8Individual/more than the g, ability formation advantage crowd after being manured into soil, and under in soil, surviving.
2) contain rich soluble organic carbon and organonitrogen in the fertilizer product, can very superior growing environment be provided, greatly improved the ability that function is grown and brought into play to antagonism bacterium deciding in soil to function yeast.
3) product organophosphorus of the present invention and organonitrogen content are high especially, all reach 4%.One of this products material is a pig manure, and total phosphorous is very high, and biological effectiveness is high especially, in this microorganism organic fertilizer product, need not to add any phosphatization fertilizer that contains, and uses to make behind this product crop spend phosphorus in seedling stage plain critical porion smoothly.By contrast, it is relatively poor to contain the phosphatization fertilizer artifact validity that is manured into soil; Organonitrogen can reach more than 4% (most of be amino acid and micromolecule polypeptide) in this product, has guaranteed the continual and steady supply of fertilizer nitrogen, make plant plant growth and anti-adversity ability strengthens greatly.
4) result of study shows, after using this product on the soil of continuous cropping capsicum, can significantly reduce the sickness rate of capsicum epidemic disease, and the biological control rate reaches more than 80%, and plant grow fine (Fig. 2).The soil of this fertilizer of continuous administration is higher to the biological control rate of continuous cropping capsicum epidemic disease.
Four, description of drawings
The dull and stereotyped antagonistic effect of Fig. 1 antagonistic strain NJLZ-8
The potted plant effect of Fig. 2 is annotated: the conventional fertilizer 3.CK+ of 1.CK (continuous cropping capsicum epidemic disease soil is arranged) 2.CK+ antagonism bacterium NJLZ-8 bacterium liquid 4.CK+ antagonism bacterium NJLZ-8 is through the fertilizer (fertilizer product of the present invention) of Secondary Fermentation
Five, embodiment
(1) separation of bacterial strain and evaluation
Gather the plant that capsicum epidemic disease has taken place, adopt the mould selective medium of epidemic disease to separate and obtain pathogenic bacteria, and verify that it is pathogenic from the strong position, boundary of the disease of the stem of morbidity capsicum.Be indicator with the capsicum epidemic disease pathogenic bacteria then, separate the antagonism bacterium in the healthy plant rhizosphere soil from the piece of continuous cropping capsicum epidemic disease morbidity field.Sieve efficient antagonistic strain again through pot experiment then, finally obtain antagonism bacterium NJLZ-8 and identify that bacterial classification is-70 ℃ of preservations in 30% glycerine.Dull and stereotyped face-off test-results finds that antagonism bacterium NJLZ-8 reaches 68% (like Fig. 1) to the inhibiting rate of pathogenic bacteria.
Antagonism bacterium NJLZ-8 belongs to bacillus pumilus (Bacillus pumilus), and its main biological characteristics is: Gram-positive (G
+), produce gemma.Cell is shaft-like, aerobic or amphimicrobian growth, and catalase is positive, oxidase negative; The starch hydrolysis is negative, the V.P. reacting positive, and methyl red test is negative, and glucose fermentation produces not aerogenesis of acid; Unfermentable wood sugar, L-arabinose, N.F,USP MANNITOL can not utilize Citrate trianion, the nitrate reductase enzyme positive; Gelatin liquefaction positive, lecithinase is negative, not decomposition of cellulose.
(2) microbial inoculum production
1) antagonism bacterium NJLZ-8 is inoculated into the PDA nutrient solution, carries out liquid fermenting production, the condition of fermentative prodn is: pH growth scope nature; 30～35 ℃ of culture temperature scopes; Stirring velocity is 180～300 rev/mins, and the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount>=1 * 10 in the fermented liquid
10Individual/ml.Used PDA nutrient solution compound method does, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling; Add the 20g common sucrose in the filtrating after filtering; Be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
2) with antagonism bacterium NJLZ-8 fermented liquid respectively with become thoroughly decomposed after pig manure and dish dregs of rice mikrobe enzymolysis mixture carry out solid fermentation respectively; Add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton; Turning every day is 1 time in the solid fermentation process; Make the solid fermentation temperature be no more than 50 ℃, ferment and finish after 5 days, make the antagonism bacterial content reach 1 * 10
8Individual/as more than the g, to obtain the pig manure microbial organic fertilizer and the dish dregs of rice enzymolysis mixture microbial organic fertilizer of solid fermentation.Pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content >=35%, and the organonitrogen quality is 1.2-2% than content, water cut mass ratio 25-30%.
The mikrobe enzymolysis preparation method of mixture of its Chinese food dregs of rice is (public, as to see patent of invention ZL200610086126.0, a kind of organism preparation method of agricultural amino acid and fertilizer product thereof); With preserving number is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material, regulates water cut 55-65% and pH 6.0-7.5, carries out open solid fermentation; Treat to begin immediately when leavening temperature rises to 50 ℃ turning, later turning every day, make the heap temperature maintain all the time 35-50 ℃ totally 5 days; After solid fermentation begins; The pH of stacking yard can constantly rise, and with containing acid solution body adjusting water cut and pH, makes them maintain 55-65% and 6.0-7.0 respectively during each turning; When stopping, fermentation sprays with containing the acid solution body again; Be adjusted into about 5.0 until stacking yard pH, carry out cryodrying or natural airing again, be the mikrobe enzymolysis mixture (containing amino acid whose mixing material) of the dish dregs of rice.
3) then with pig manure microbial organic fertilizer and 60: 40 by volume ratio thorough mixing after-ripening of dish dregs of rice enzymolysis mixture microbial organic fertilizer 2 days; Turning is 2 times in the after-ripening process; With the water cut of after-ripening thing cryodrying to 30%, packing is dispatched from the factory and is the microbial organic fertilizer product that can prevent and treat the continuous cropping capsicum epidemic disease.
The above-mentioned continuous cropping capsicum epidemic disease microorganism organic fertilizer of preventing and kill off contains 1 * 10 in this fertilizer product
8The above antagonism bacterium NJLZ-8 of individual/g, full nitrogen quality are that 4～5% (being organonitrogen 90% or more), total nitrogen phosphorus potassium nutrition mass ratio are 6～10%, the quality of organic matter ratio content is 30～35% than content.
(3) pot experiment
In the pot experiment, capsicum variety is Soviet Union green pepper No. five, when adopting healthy paddy rice seedlings and soil to 6～8 true leaves, transplants to little plastic tub.The serious soil of continuous cropping capsicum epidemic disease picks up from the Funan County, Anhui.The serious soil 3kg of capsicum epidemic disease is made in every basin load, and antagonism bacterium NJLZ-8 bacterium liquid is handled with 10
8Cfu/g soil is evenly admixed in the continuous cropping capsicum epidemic disease soil, and fertilizer product of the present invention is evenly admixed in the sick soil with 2% usage quantity (w/w).Each handles 3 groups of repetitions, and every group is repeated 6 strain capsicums.Keep respectively handling humidity in the soil, temperature unanimity during cultivation.After transplanting 35d, the statistics incidence is measured each item index.
The result shows that the sickness rate of continuous cropping soil is 88%, and survival plant growing way is short and small, poor growth; The sickness rate that inoculation antagonism bacterium NJLZ-8 bacterium liquid is handled in the continuous cropping soil is 25%, and control rate reaches 72%, and using the sickness rate that fertilizer product of the present invention handles in the continuous cropping soil is 16%, and control rate reaches 82% (table 1), and plant grow fine (Fig. 2).Explain that this product can effectively suppress the generation of capsicum epidemic disease, improve the control effect of the control of capsicum epidemic disease.
The yield of hot pepper of control treatment is merely 4gpot in the continuous cropping soil
-1, and use output that fertilizer product of the present invention handles in the continuous cropping soil for being 43.92gpot
-1, raising the output reaches 9.95 times.The capsicum overground part fresh weight of continuous cropping soil is the 19.60g/ strain, and dry weight is the 4.10g/ strain, is the 43.92g/ strain and use the capsicum fresh weight that fertilizer product of the present invention handles in the continuous cropping soil, and dry weight is 12.91g/ strain (table 2), the living weight significant difference; Use fertilizer product of the present invention in the continuous cropping soil and handle than control treatment, blade Net Photosynthetic Rate, transpiration rate significantly increase (table 3), help the accumulation and the storage of plant photosynthesis product, thereby improve yield of hot pepper and living weight.These results show that this product can also reduce or eliminate pathogenic bacteria to the restraining effect that chili growth produces except the generation that can effectively reduce capsicum epidemic disease, promote the growth of capsicum significantly.
Table 1 pot experiment capsicum epidemic disease sickness rate and control effect
Table 2 pot experiment yield of hot pepper and living weight
The photosynthetic index of table 3 pot experiment capsicum functional leaf
Patent of the present invention is set about from this key issue of micro-flora of continuous cropping soil, develops a kind of microorganism organic fertilizer product that can significantly reduce continuous cropping capsicum epidemic disease sickness rate, and control rate reaches more than 80%.The mechanism of action of this fertilizer product be function yeast that this fertilizer product provides and effectively carbon and effective nitrogenous source to the cultivation of continuous cropping obstacle soil useful microbe fauna good condition is provided, make it to recover rapidly ecology and the food chain thereof of indigenous useful microbe and external source function yeast mikrobe; On the other hand, contain the organonitrogen and the organophosphorus of high level in this fertilizer product, these nutritive substances are very favourable to the growth of crop in the continuous cropping obstacle soil, improved upright seedling rate and the anti-adversity ability of crop greatly.
Claims (6)
1. antagonism bacterium NJLZ-8 that can prevent and treat the continuous cropping capsicum epidemic disease; Belong to bacillus pumilus (Bacillus pumilus); Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 9th, 2009, culture presevation number is CGMCC NO.3182;
The main biological characteristics of NJLZ-8 is: Gram-positive G
+, producing gemma, cell is shaft-like, aerobic or amphimicrobian growth; Catalase is positive, oxidase negative, and the starch hydrolysis is negative, the V.P. reacting positive; Methyl red test is negative, and glucose fermentation produces not aerogenesis of acid, and unfermentable wood sugar, L-arabinose, N.F,USP MANNITOL can not utilize Citrate trianion; The nitrate reductase enzyme positive, gelatin liquefaction positive, lecithinase is negative, not decomposition of cellulose.
2. the microorganism organic fertilizer of the control continuous cropping capsicum epidemic disease of producing with the said antagonism bacterium of claim 1 is characterized in that: contain 1 * 10 in this fertilizer
8The antagonism bacterium NJLZ-8 that individual/g is above, full nitrogen quality is 4～5% than content, and mass ratio is organonitrogen more than 90% in the full nitrogen, and total nitrogen phosphorus potassium nutrition mass ratio is 6～10%, and the quality of organic matter ratio content is 30～35%.
3. the working method of the said microorganism organic fertilizer of claim 2 comprises:
1) the described antagonism bacterium of claim 1 NJLZ-8 is inoculated into the PDA nutrient solution; Carry out liquid fermenting production, the condition of fermentative prodn is: 30～35 ℃ of culture temperature scopes, stirring velocity are 180～300 rev/mins; The fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount>=1 * 10 in the fermented liquid
10Individual/ml;
2) with antagonism bacterium NJLZ-8 fermented liquid respectively with become thoroughly decomposed after pig manure and dish dregs of rice mikrobe enzymolysis mixture carry out solid fermentation; Add NJLZ-8 fermented liquid 50L in the SOLID ORGANIC material per ton; Turning every day is 1 time in the solid fermentation process; Make the solid fermentation temperature be no more than 50 ℃, ferment and finish after 5 days, make antagonism bacterium NJLZ-8 content reach 1 * 10
8Individual/as more than the g, to obtain pig manure microbial organic fertilizer and dish dregs of rice enzymolysis mixture microbial organic fertilizer; The mikrobe enzymolysis preparation method of mixture of its Chinese food dregs of rice does, is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material with preserving number, regulates water cut 55-65% and pH 6.0-7.5; Carry out open solid fermentation, treat to begin immediately when leavening temperature rises to 50 ℃ turning, later turning every day; Make the heap temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation began, the pH of stacking yard can constantly rise; During each turning with containing acid solution body adjusting water cut and pH; Make them maintain 55-65% and 6.0-7.0 respectively, spray with containing the acid solution body when fermentation stops again, pH is adjusted into 5.0 until stacking yard; Carry out cryodrying or natural airing again, be the mikrobe enzymolysis mixture of the dish dregs of rice;
3) then with the pig manure microbial organic fertilizer of solid fermentation and 60: 40 by volume ratio thorough mixing after-ripening of dish dregs of rice enzymolysis mixture microbial organic fertilizer 2 days; Turning is 2 times in the after-ripening process; With the water cut of after-ripening thing cryodrying to 30%, packing is dispatched from the factory and is the microbial organic fertilizer that can prevent and kill off the continuous cropping capsicum epidemic disease.
4. according to the working method of microorganism organic fertilizer described in the claim 3; Wherein the used PDA nutrient solution of step 1) compound method is: be cut into small pieces to be put in the water after peeling with the 200g potato and boil; Boil 30min after the boiling, add the 20g common sucrose in the filtrating after filtering, be settled to 1000ml; The pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
5. according to the working method of claim 3 or 4 said microorganism organic fertilizers; Step 2 wherein) the pig manure germination index after used the becoming thoroughly decomposed is more than 98%; The fertilizer quality is than content >=35%, and the organonitrogen quality is 1.2-2% than content, water cut mass ratio 25-30%.
6. the application of the said microorganism organic fertilizer of claim 2 in control continuous cropping capsicum epidemic disease.
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| CN103484405B (en) * | 2013-09-22 | 2015-03-04 | 长治学院 | Taxus chinensis rhizosphere high-efficiency phosphate-solubilizing stenortrophomonas maltophilia and application thereof |
| CN103468618B (en) * | 2013-09-25 | 2015-04-01 | 上海交通大学 | Cellulose-degrading bacteria with phosphate-dissolving capability and application thereof |
| CN104230582A (en) * | 2014-09-11 | 2014-12-24 | 红云红河烟草(集团)有限责任公司 | Biological organic fertilizer containing bacillus pumilus |
| CN104961538B (en) * | 2015-05-08 | 2017-11-14 | 淮阴工学院 | Recessed soil matrix immobilization complex micro organism fungicide for improveing capsicum continuous cropping obstacle and preparation method thereof |
| CN105052466A (en) * | 2015-07-31 | 2015-11-18 | 淮阴工学院 | Method of crop rotation of cayenne peppers and green soy beans after autumn delay for eliminating continuous cropping obstacle |
| CN107176873A (en) * | 2017-05-09 | 2017-09-19 | 湖南泰谷生物科技股份有限公司 | Biological organic fertilizer and its preparation method and application |
| CN108675885A (en) * | 2018-08-10 | 2018-10-19 | 保康丰源肥业有限责任公司 | A kind of biological organic fertilizer and preparation method thereof of prevention capsicum epidemic disease |
| CN109197424A (en) * | 2018-11-14 | 2019-01-15 | 江苏沿海地区农业科学研究所 | A kind of crop rotation implantation methods of prevention and control capsicum continuous cropping obstacle |
| CN115838668B (en) * | 2022-11-29 | 2024-06-18 | 安徽科技学院 | Preparation method of pig manure composite fermentation microbial inoculum, fermentation microbial inoculum prepared by using same, pig manure bio-organic fertilizer and preparation method and application thereof |
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| CN1597642A (en) * | 2004-07-27 | 2005-03-23 | 南京农业大学 | Fertilizer capable of eliminating crop continuous cropping obstruct and its production method |
| CN101671633A (en) * | 2009-09-28 | 2010-03-17 | 南京农业大学 | Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof |
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| CN1597642A (en) * | 2004-07-27 | 2005-03-23 | 南京农业大学 | Fertilizer capable of eliminating crop continuous cropping obstruct and its production method |
| CN101671633A (en) * | 2009-09-28 | 2010-03-17 | 南京农业大学 | Antagonistic bacteria for preventing and eliminating greensickness of continuous cropping cotton and microbial organic fertilizer thereof |
| CN101805716A (en) * | 2010-04-02 | 2010-08-18 | 南京农业大学 | Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof |
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