CN101864470A - Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells - Google Patents
Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells Download PDFInfo
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- CN101864470A CN101864470A CN 201010197762 CN201010197762A CN101864470A CN 101864470 A CN101864470 A CN 101864470A CN 201010197762 CN201010197762 CN 201010197762 CN 201010197762 A CN201010197762 A CN 201010197762A CN 101864470 A CN101864470 A CN 101864470A
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Abstract
The invention belongs to the field of the submerged fermentation of microalgae cells, in particular to a method for increasing the yield of extracellular polysaccharide produced by the submerged fermentation of long thread moss cells. The method for increasing the yield of the extracellular polysaccharide of the long thread moss cells by controlling illumination intensity by stages and by a culture medium fermentation process has simple process and high production efficiency and is suitable for amplifying industrial production, and the polysaccharide of the long thread moss cells obtained by production can be used as a natural antiviral medicine or a food raw material after being separated and purified.
Description
(1) technical field
The invention belongs to the submerged fermentation field of microalgae cell, particularly a kind of method that improves yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells.
(2) background technology
Delivering vegetables is the economic blue-green algae of a kind of land natural disposition that is grown in NORTHWEST CHINA portion and northern arid area, has very high nutritive value.Because its growing environment is abominable, hair weeds cells to a large amount of gelatinoid of cell exocrine, is wrapped in outside hair weeds cells and the filament in process of growth, forms gelatinous sheath, and the protection hair weeds cells is not subjected to the harm of arid, high temperature, uv-radiation.The main component of colloid is a polysaccharide, and these polysaccharide materials are resisted poor environment vegetatively to delivering vegetables, and ensures that the normal growth of hair weeds cells has important biological meaning.
Discover that the acidic polysaccharose-nosoflan that extracts has very strong antiviral activity to multiple viruses with big envelope such as influenza virus, human cytomegalic inclusion disease virus, monospore exanthema viruses, is a kind of potential antiviral from wild hair weeds cells; And the polysaccharide of delivering vegetables of liquid submerged fermentation production has high similarity with the natural laver polysaccharide aspect physico-chemical property, apparent pattern and the ntiviral characteristic, can substitute the raw material that the natural laver polysaccharide is used for suitability for industrialized production, be a kind of novel product polysaccharide resource.
Hair weeds cells liquid submerged fermentation method is produced the polysaccharide of delivering vegetables, and with short production cycle, cost is low, does not need to consume wild hair-like seaweed resource, meets the policy of state guarantee hair-like seaweed resource ecology.At present, the production STUDY ON POLYSACHAROSE of delivering vegetables mainly concentrates on Optimizing Conditions of Fermentation for the hair weeds cells tank fermentation method, as Optimum of culture medium, and the optimization of culture condition; Do not see the pertinent literature report and utilize the control culture condition to improve the polysaccharide yield of delivering vegetables.
(3) summary of the invention
The present invention provides a kind of and has realized that easy, outstanding effect improves the method for yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells in order to remedy the deficiencies in the prior art.
The present invention is achieved through the following technical solutions:
A kind of method that improves yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells is a raw material with the hair weeds cells, mainly comprises the steps:
(1) fs cultivated 4 days, adopt add glucose nitrogenous source BG-11 substratum arranged, intensity of illumination is controlled at 40～60 μ mol/m
2S;
(2) subordinate phase was cultivated 3 days, adopted the compound no nitrogenous source BG-11 substratum that adds glucose and sodium acetate, and intensity of illumination is controlled at 60～160 μ mol/m
2S.
In the step (1), the concentration of described glucose is 4g/L; In the step (2), described sodium acetate concentration is 2g/L, and glucose concn is 3～4g/L, medium pH 〉=9, and intensity of illumination is controlled at 100～160 μ mol/m
2S.
Hair weeds cells is a ptotoautotroph, also can carry out mixotrophism and heterotrophic growth, intensity of illumination is the material impact factor in its process of growth, for the polysaccharide of delivering vegetables, especially be secreted into the exocellular polysaccharide in the substratum, be the primary metabolite in the hair weeds cells process of growth, increase very fast in the later stage, therefore by controlling intensity of illumination and medium component stage by stage, the growth that improve hair weeds cells early stage, the later stage is adopted the deliver vegetables high speed accumulation of polysaccharide of the nitrogen-free agar of compounded carbons.
Adopt Technology of the present invention, the hair weeds cells polysaccharide yield is significantly improved, and its output is brought up to 3～4g/L.
Technology of the present invention is simple, and the production efficiency height can significantly improve the output of hair weeds cells polysaccharide, is suitable for amplifying suitability for industrialized production, produces the hair weeds cells polysaccharide that obtains, and can be used as natural antiviral drug or food supplementary material after separation and purification.
(4) embodiment
Embodiment 1: comparative examples
Hair weeds cells kind of the present invention is that (it is tall long bright that Chinese patent ZL031191101.0, contriver: Jia Shiru Soviet Union build space, authorizes day: on March 8th, 2006) for the own separating and preserving of Tianjin industrial microorganism key lab.
This invention separates the hair weeds cells kind that obtains preservation for a long time from the wild frond of delivering vegetables, carry out the hair weeds cells suspension culture, adopt high-density culture technology, add organic carbon source the mixotrophism pattern (Chinese patent 2006100113589.4, it is virtuous forever that contriver: Jia Shiru builds the space woods in seapeak Soviet Union, open), adopt the BG-11 substratum that adds glucose, cultivated 24 ℃ of temperature 7 days, rotating speed 140rpm, intensity of illumination 40 ~ 90 μ mol/m
2S, the polysaccharide yield of delivering vegetables 2.25g/L.
Embodiment 2:
The illumination reaction device adopts and controls intensity of illumination and culture method stage by stage, and conditions such as temperature, rotating speed are with comparative examples 1.
4 days of fs adopt in fermentation 4g/L glucose nitrogen BG-11 substratum arranged, intensity of illumination is controlled at 40 ~ 60 μ mol/m
2S, at the 5th day that cultivates, the no nitrogen BG-11 substratum of feed supplement 2g/L sodium-acetate and 3～3.5g/L glucose was regulated more than the pH9.0, and the liquid level average light is shone intensity control at 100～160 μ mol/m
2S.
With this control strategy production polysaccharide of delivering vegetables stage by stage, adopting ethanol precipitation to obtain polysaccharide yield is 3.5g/L.
Embodiment 3:
The illumination reaction device adopts and controls intensity of illumination and culture method stage by stage, and conditions such as temperature, rotating speed are with comparative examples 1.
At the aerobic BG-11 substratum that adopted the glucose that adds 4g/L in 4 days of submerged fermentation fs, intensity of illumination is controlled at 40～60 μ mol/m
2S is cultivating the 5th day harvested cell, all changes in the no nitrogen BG-11 substratum that adds 2g/L sodium-acetate and 4g/L glucose, regulates medium pH more than 9.0, and intensity of illumination (liquid level mean value) is controlled at 100～160 μ mol/m
2S.
With this control strategy production polysaccharide of delivering vegetables stage by stage, it is 4g/L that two stage fermentation liquid adopt ethanol precipitation to obtain polysaccharide yield.
Claims (4)
1. a method that improves yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells is a raw material with the hair weeds cells, it is characterized in that: mainly comprise the steps:
(1) fs cultivated 4 days, adopt add glucose nitrogenous source BG-11 substratum arranged, intensity of illumination is controlled at 40～60 μ mol/m
2S;
(2) subordinate phase was cultivated 3 days, adopted the compound no nitrogenous source BG-11 substratum that adds glucose and sodium acetate, and intensity of illumination is controlled at 60～160 μ mol/m
2S.
2. the method for raising yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells according to claim 1 is characterized in that: in the step (1), the concentration of described glucose is 4g/L.
3. the method for raising yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells according to claim 1 and 2 is characterized in that: in the step (2), described sodium acetate concentration is 2g/L, and glucose concn is 3～4g/L, medium pH 〉=9.
4. the method for raising hair weeds cells stature fermentative production exopolysaccharides according to claim 1 and 2 is characterized in that: in the step (2), described intensity of illumination is controlled at 100～160 μ mol/m
2S.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010197762 CN101864470B (en) | 2010-06-11 | 2010-06-11 | Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells |
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| CN 201010197762 CN101864470B (en) | 2010-06-11 | 2010-06-11 | Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells |
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| CN101864470A true CN101864470A (en) | 2010-10-20 |
| CN101864470B CN101864470B (en) | 2013-01-09 |
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| CN 201010197762 Expired - Fee Related CN101864470B (en) | 2010-06-11 | 2010-06-11 | Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559799A (en) * | 2011-01-27 | 2012-07-11 | 河北工业大学 | Preparation method for algae endophytic fungi exocellular polysaccharide |
| CN102669520A (en) * | 2012-05-07 | 2012-09-19 | 天津科技大学 | Nostoc flagelliforme polysaccharide oral liquid and preparation method thereof |
| CN108531420A (en) * | 2018-03-19 | 2018-09-14 | 天津科技大学 | Method that is a kind of while improving hair weeds cells density and polysaccharide yield |
| CN113181222A (en) * | 2021-04-26 | 2021-07-30 | 武汉大学 | Application of extracellular polysaccharide metabolite of cryptococcus lactis in resisting viruses |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1530439A (en) * | 2003-03-14 | 2004-09-22 | 天津科技大学 | Hair weeds bacterial culture and culturing product |
| CN101063085A (en) * | 2006-04-29 | 2007-10-31 | 天津科技大学 | Method for black moss cell high-density culture of stable high-yield black moss polysaccharide |
-
2010
- 2010-06-11 CN CN 201010197762 patent/CN101864470B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1530439A (en) * | 2003-03-14 | 2004-09-22 | 天津科技大学 | Hair weeds bacterial culture and culturing product |
| CN101063085A (en) * | 2006-04-29 | 2007-10-31 | 天津科技大学 | Method for black moss cell high-density culture of stable high-yield black moss polysaccharide |
Non-Patent Citations (2)
| Title |
|---|
| 《J Appl Phycol》 20070901 Jianyu Su et al Morphology, cell growth, and polysaccharide production of Nostoc flagelliforme in liquid suspension culture at different agitation rates 213－217 1－4 第20卷, 2 * |
| 《J Appl Phycol》 20080523 Haifeng Yu et al Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation 127－133 1－4 第21卷, 2 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559799A (en) * | 2011-01-27 | 2012-07-11 | 河北工业大学 | Preparation method for algae endophytic fungi exocellular polysaccharide |
| CN102669520A (en) * | 2012-05-07 | 2012-09-19 | 天津科技大学 | Nostoc flagelliforme polysaccharide oral liquid and preparation method thereof |
| CN108531420A (en) * | 2018-03-19 | 2018-09-14 | 天津科技大学 | Method that is a kind of while improving hair weeds cells density and polysaccharide yield |
| CN113181222A (en) * | 2021-04-26 | 2021-07-30 | 武汉大学 | Application of extracellular polysaccharide metabolite of cryptococcus lactis in resisting viruses |
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|---|---|
| CN101864470B (en) | 2013-01-09 |
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