CN101766251A - Method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood - Google Patents
Method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood Download PDFInfo
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- CN101766251A CN101766251A CN200810154691A CN200810154691A CN101766251A CN 101766251 A CN101766251 A CN 101766251A CN 200810154691 A CN200810154691 A CN 200810154691A CN 200810154691 A CN200810154691 A CN 200810154691A CN 101766251 A CN101766251 A CN 101766251A
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Abstract
The invention discloses a method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood, comprising the following steps of: taking fresh and healthy pig blood as a raw material; carrying out anticoagulation treatment, centrifuging and separation on the fresh and healthy pig blood to obtain a blood cell and blood plasma; carrying out high-pressure cutting and wall breaking, complex enzyme hydrolysis and flashing and deodorizing process treatment on the blood cell to obtain enzymolysis liquid; carrying out rough filtration, fine filtration, condensation treatment and spray drying to obtain the bioactive peptide for enriching the blood; and carrying out modification treatment on the separated blood plasma to obtain the plasma protein powder. The invention has the following advantages that: 1. the production cost is low and no pollution to the environment is generated; 2. the average molecular weight of small peptide of the produced bioactive peptide for enriching the blood is not more than 10,000Dalton, the content of free amino acid is not more than 15.0percent and the protein of the plasma protein powder is not less than 90.0 percent; 3. the water content of the produced modified plasma protein is not more than 5.0 percent, the viscosity (5 percent solution) is not less than 2.0 mpa.s and the content of the protein is not less than 90.0 percent; and 4. the process method saves time and energy, has simple operation and fully utilizes pig blood resources.
Description
Technical field:
The present invention relates to a kind of method of from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood.
Background technology:
, start from the beginning of last century both at home and abroad to the exploitation of livestock and poultry blood according to relevant bibliographical information.Be mainly used in feed industry in early days.Along with technical development, the food expert of states such as Germany, Denmark with the pig blood system from going out blood plasma as tonic, add in sausage, pudding, the cake; The seventies Sweden, France begin fresh pig blood is used for sausage and pudding, and it is clear with the replacement egg that a spot of pig blood plasma is used for products such as cake, biscuit, bread, vermicelli, but have also only utilized 25%～30% of pig blood resource.Pig blood also once was used to the binder of furniture, and the fining agent in the brewing industry and be that raw material extracts many goods that physiological function is arranged with pig blood is as ferroheme, superoxide dismutase, fibrin ferment etc.France begins that again blood plasma is made plasma powder and albumen powder adds in the food in recent years, but these application are still very limited.
Present China utilizes the skill level of blood lower, product is dull, mainly be to produce dry blood meal, fermented blood meal feed etc., developed blood bean curd, hematinic etc. in recent years again, but its digestibility only have about 30%～50%, utilization rate is very low, and the production environment improvement of still needing.Though domesticly in the utilization of pig blood resource, done a large amount of element tasks, still be in the experimental study stage, as adopting acid hydrolysis process, and be single product and extract in the porcine haemoglobin hydrolysis.In the application of field of food, the research report is also arranged at present, technological difficulties are the pollution problem again that a large amount of uses of organic solvent produce, owing to these reasons, the comprehensive utilization of pig blood resource is difficult to realize industrialization.
Summary of the invention:
Purpose of the present invention just is to overcome above-mentioned the deficiencies in the prior art, and provide a kind of method of from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood, this method is with the complex enzyme for hydrolyzing hemoglobin production active peptide of enriching blood, adopt TGase enzymolysis plasma protein to produce modified plasma protein, have and save time, economize energy, simple to operate, advantage that resource utilization is high.
Technical scheme of the present invention is: a kind of method of extracting modified plasma protein powder, the active peptide of enriching blood from pig blood is characterized in that: with fresh healthy swine blood is raw material, carries out centrifugation after anti-freezing is handled, and obtains haemocyte and blood plasma; Haemocyte is sheared the enzymolysis liquid that obtains after broken wall, complex enzyme for hydrolyzing, the processing of flash distillation deodorizing technology through high pressure, and through coarse filtration, smart filter and concentration, spray-drying is made the active peptide of enriching blood again; Then make modified plasma protein powder through the blood plasma after separating through the modification processing.
The technical process of above-mentioned anti-freezing processing and centrifugation is: add people's natrium citricum 0.33% (w/v) in new blood, then with it at 5 ℃, 3000 * g centrifugation 20min, obtain erythrocyte after the separation of supernatant, adopt homogenizer broken erythrocyte under 60MPa pressure immediately, make hemoglobin stripping and homogenate, freezing standby, supernatant is a blood plasma, and blood plasma accounts for 60% of whole blood, is cooled to 1 ℃～2 ℃ preservations.
Blood plasma after the above-mentioned separation is then handled the technical process of making modified plasma protein powder through modification: (1) adds the gelation that TGase improves plasma protein in blood plasma; The condition that improves the gelation of plasma protein is: the addition of TGase between 1-5u/g, pH7.0-9.0, reaction temperature be that 45 ℃-55 ℃, substrate protein concentration are 12%; (2) plasma protein after the modification is concentrated, its concentrated condition is: vacuum 0.13MPa, and temperature is not higher than 55 ℃, makes solid content concentrate most 40%; (3) above-mentioned concentrate is carried out spray-drying, obtain SDPP; Spray-dired EAT is 160 ℃～170 ℃, and temperature of outgoing air is 48 ℃～50 ℃, and input concentration is about 40%.
The above-mentioned technical process of making the active peptide of enriching blood by erythrocyte is: it is 8% that the hemoglobin after will handling is transferred its concentration, be heated to 40 ℃-55 ℃ insulation hydrolysis, add 0.2% the compound protease hemoglobin that is hydrolyzed, the pH value is between 7-8, add decolorizer after the hydrolysis, at room temperature handle the centrifugal 10min of 3000 * g behind the 30min, obtain the enzymolysis supernatant, subnatant is a red blood cell; The red blood cell of lower floor uses ultrasonic wave homogenate technology after cleaning, make hematoclasis, prepares denatured hemoglobin, and again through centrifugation, sediment is made the hemoglobin powder through concentrated and spray-drying; The supernatant of enzymolysis carries out making the active peptide of enriching blood through spray-drying after essence is filtered, concentrated through after the coarse filtration.
Above-mentioned complex enzyme adopts AS1.39 neutral proteinase and papain to get according to 5: 5 ratio is mixed.
The optimal conditions of above-mentioned complex enzyme for hydrolyzing hemoglobin is: 55 ℃ of temperature, pH7.5 value, E/S are that 6000-8000u/g, concentration of substrate are 6-8%, enzymolysis 6-8h.
The membrane filtration condition of the above-mentioned active peptide of enriching blood is: hold back 10000DA above peptide and macromolecular substances with the UF-3 film, membrane filtration pressure is 2.0mPa, and membrane flux is 16.3L/m
2.h; The suitable condition that the vacuum of active peptide of enriching blood concentrates is: 70 ℃ of thickening temperatures, vacuum 0.090mPa; The spray-drying of active peptide of enriching blood adopts centrifugal spray drier, 300 ℃ of its EATs, and 90 ℃～100 ℃ of temperature of outgoing airs, under the condition of charging flow velocity 2L/h, active peptide drying efficiency 145g/L enriches blood.
The present invention has following advantage and good effect:
1, because this technology does not adopt organic solvent, not only reduced cost but also alleviated discharging, environmentally safe.
2, the active peptide quality height of enriching blood, the color and luster of the production of employing complex enzyme for hydrolyzing hemoglobin are white, quality is fine and smooth evenly, little peptide mean molecule quantity≤10000Dalton, free aminoacid content≤15.0%.Hemalbumin powder: protein 〉=90.0%.This product has good preventive and therapeutic effect to hypoferric anemia, its absorptivity height, be inorganic benefit chalybeate absorptivity 4-5 doubly, and without any side effects.
3, the modified plasma protein that adopts TGase enzymolysis plasma protein to produce, nonhygroscopic, do not lump, no fishy smell.Its moisture≤5.0%, viscosity (5% solution) 〉=2.0 milli handkerchief second, protein 〉=90.0%.This modified plasma protein powder is used for meat products processing, and the particularly processing of intestines based article can replace or part replaces phosphate to play the water conservation tackify, can not influence the absorption of human body to calcium, has improved the local flavor and the nutritive value of meat products simultaneously.
4, process save time, economize energy, simple to operate, made full use of the pig blood resource.
Description of drawings:
Fig. 1 is a process chart of the present invention.
The specific embodiment:
A kind of method of extracting modified plasma protein powder, the active peptide of enriching blood from pig blood may further comprise the steps:
1, the preparation of hemoglobin (HC) and blood plasma.
Add people's natrium citricum 0.33% (w/v) in the fresh pig blood, in case hemostasis liquid condenses, then with it at 5 ℃, 3000 * g centrifugation 20min, obtaining its protein content of erythrocyte after the separation of supernatant is (33.12 soil 2.57) g/100g, adopt homogenizer broken erythrocyte under 60MPa pressure immediately, make hemoglobin stripping and homogenate, freezing standby.Supernatant is a blood plasma, and blood plasma accounts for 60% of whole blood, is cooled to 1 ℃～2 ℃ preservations.
2, the blood plasma after will separating is handled through bio-modification and is made modified plasma protein powder.Its technical process is: (1) adds the gelation that TGase improves plasma protein in blood plasma; The condition that improves the gelation of plasma protein is: the addition 5u/g of TGase, pH8.0, reaction temperature are that 45 ℃, substrate protein concentration are 12%; (2) plasma protein after the modification is concentrated, its concentrated condition is: vacuum 0.13MPa, and temperature is not higher than 55 ℃, makes solid content concentrate most 40%; (3) above-mentioned concentrate is carried out spray-drying, obtain nonhygroscopic, do not lump, the yellow SDPP of no fishy smell; Spray-dired EAT is 160 ℃～170 ℃, and temperature of outgoing air is 48 ℃～50 ℃, and input concentration is about 40%.
3, make the active peptide of enriching blood by erythrocyte.Its technical process is: it is 8% that the hemoglobin after will handling is transferred its concentration, be heated to 50 ℃ of insulation hydrolysis, with the hemoglobin Mass Calculation, add 0.2% the compound protease hemoglobin that is hydrolyzed, increase gradually owing to amino nitrogen content in the hydrolytic process, pH value in the reactant liquor is descended, can keep its pH value by dripping 4N NaOH, after stirring hydrolysis 24h, be warming up to 100 ℃ of water-bath 5min enzyme that goes out, add decolorizer (sodium carboxymethyl starch) then, at room temperature handle the centrifugal 10min of 3000 * g behind the 30min, obtain the enzymolysis supernatant, subnatant is a red blood cell.The red blood cell of lower floor uses ultrasonic wave homogenate technology after cleaning, make hematoclasis, prepares denatured hemoglobin, and again through centrifugation, sediment is made the hemoglobin powder through concentrated and spray-drying; The supernatant of enzymolysis carries out making the active peptide of enriching blood through spray-drying after essence is filtered, concentrated through after the coarse filtration.
Above-mentioned complex enzyme adopts AS1.39 neutral proteinase and papain to get according to 5: 5 ratio is mixed.
The optimal conditions of above-mentioned complex enzyme for hydrolyzing hemoglobin is: 55 ℃ of temperature, pH7.5 value, E/S are that 8000u/g, concentration of substrate are 8%, enzymolysis 8h.
The membrane filtration condition of the above-mentioned active peptide of enriching blood is: hold back 10000DA above peptide and macromolecular substances with the UF-3 film, membrane filtration pressure is 2.0mPa, and membrane flux is 16.3L/m
2.h; The suitable condition that the vacuum of active peptide of enriching blood concentrates is: 70 ℃ of thickening temperatures, vacuum 0.090mPa; The spray-drying of active peptide of enriching blood adopts centrifugal spray drier, 300 ℃ of its EATs, and 90 ℃～100 ℃ of temperature of outgoing airs, under the condition of charging flow velocity 2L/h, active peptide drying efficiency 145g/L enriches blood.
Claims (7)
1. method of from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood, it is characterized in that: with fresh healthy swine blood is raw material, carries out centrifugation after anti-freezing is handled, and obtains haemocyte and blood plasma; Haemocyte is sheared the enzymolysis liquid that obtains after broken wall, complex enzyme for hydrolyzing, the processing of flash distillation deodorizing technology through high pressure, and through coarse filtration, smart filter and concentration, spray-drying is made the active peptide of enriching blood again; Then make modified plasma protein powder through the blood plasma after separating through the modification processing.
2. the method for from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood according to claim 1, it is characterized in that: the technical process of above-mentioned anti-freezing processing and centrifugation is: add people's natrium citricum 0.33% (w/v) in new blood, then with it at 5 ℃, 3000 * g centrifugation 20min, obtain erythrocyte after the separation of supernatant, adopt homogenizer broken erythrocyte under 60MPa pressure immediately, make hemoglobin stripping and homogenate, freezing standby, supernatant is a blood plasma, blood plasma accounts for 60% of whole blood, is cooled to 1 ℃～2 ℃ preservations.
3. the method for extracting modified plasma protein powder, the active peptide of enriching blood from pig blood according to claim 1 is characterized in that: the blood plasma after the above-mentioned separation is handled the technical process of making modified plasma protein powder through modification and is: (1) adds the gelation that TGase improves plasma protein in blood plasma; The condition that improves the gelation of plasma protein is: the addition of TGase between 1-5u/g, pH7.0-9.0, reaction temperature be that 45 ℃-55 ℃, substrate protein concentration are 12%; (2) plasma protein after the modification is concentrated, its concentrated condition is: vacuum 0.13MPa, and temperature is not higher than 55 ℃, makes solid content concentrate most 40%; (3) above-mentioned concentrate is carried out spray-drying, obtain SDPP; Spray-dired EAT is 160 ℃～170 ℃, and temperature of outgoing air is 48 ℃～50 ℃, and input concentration is about 40%.
4. the method for from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood according to claim 1, it is characterized in that: the above-mentioned technical process of making the active peptide of enriching blood by erythrocyte is: it is 8% that the hemoglobin after will handling is transferred its concentration, be heated to 40 ℃-55 ℃ insulation hydrolysis, add 0.2% the compound protease hemoglobin that is hydrolyzed, the pH value is between 7-8, add decolorizer after the hydrolysis, at room temperature handle the centrifugal 10min of 3000 * g behind the 30min, obtain the enzymolysis supernatant, subnatant is a red blood cell; The red blood cell of lower floor uses ultrasonic wave homogenate technology after cleaning, make hematoclasis, prepares denatured hemoglobin, and again through centrifugation, sediment is made the hemoglobin powder through concentrated and spray-drying; The supernatant of enzymolysis carries out making the active peptide of enriching blood through spray-drying after essence is filtered, concentrated through after the coarse filtration.
5. the method for extracting modified plasma protein powder, the active peptide of enriching blood from pig blood according to claim 4 is characterized in that: above-mentioned complex enzyme adopts AS1.39 neutral proteinase and papain to get according to 5: 5 ratio is mixed.
6. the method for from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood according to claim 4, it is characterized in that: the optimal conditions of above-mentioned complex enzyme for hydrolyzing hemoglobin is: 55 ℃ of temperature, pH7.5 value, E/S are that 6000-8000u/g, concentration of substrate are 6-8%, enzymolysis 6-8h.
7. the method for from pig blood, extracting modified plasma protein powder, the active peptide of enriching blood according to claim 4, it is characterized in that: the membrane filtration condition of the above-mentioned active peptide of enriching blood is: hold back 10000DA above peptide and macromolecular substances with the UF-3 film, membrane filtration pressure is 2.0mPa, and membrane flux is 16.3L/m
2.h; The suitable condition that the vacuum of active peptide of enriching blood concentrates is: 70 ℃ of thickening temperatures, vacuum 0.090mPa; The spray-drying of active peptide of enriching blood adopts centrifugal spray drier, 300 ℃ of its EATs, and 90 ℃～100 ℃ of temperature of outgoing airs, under the condition of charging flow velocity 2L/h, active peptide drying efficiency 145g/L enriches blood.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125164A (en) * | 2010-10-29 | 2011-07-20 | 成都天屹生物科技有限公司 | Preparation method of pig blood fibrin polypeptide powder |
| CN102488078A (en) * | 2011-11-15 | 2012-06-13 | 天津宝迪农业科技股份有限公司 | Preparation method for decolorized blood globulin powder |
| CN102599331A (en) * | 2011-01-20 | 2012-07-25 | 天津宝迪农业科技股份有限公司 | Preparation method for high-protein low-ash animal plasma protein powder |
| CN103039693A (en) * | 2013-01-09 | 2013-04-17 | 合肥工业大学 | Preparation method of modified livestock and poultry plasma protein powder |
| CN103275191A (en) * | 2013-06-19 | 2013-09-04 | 天津兰瑞生物技术有限公司 | Method for largely and quick extracting tachyplesin peptide |
| CN103431155A (en) * | 2013-09-16 | 2013-12-11 | 陆杰坤 | Production technology for enzymolysis and decoloration of spray-dried animal blood cells |
| CN103589770A (en) * | 2013-10-14 | 2014-02-19 | 四川农业大学 | Industrial production method of low peptides and amino acids through enzymolysis of pig whole blood |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN104211755A (en) * | 2014-08-21 | 2014-12-17 | 广州天赐福音生物科技有限公司 | Method for extracting biologically active peptides (BAPs) in livestock and poultry blood as well as BAPs and application thereof |
| CN104256199A (en) * | 2014-10-16 | 2015-01-07 | 浙江索纳克生物科技有限公司 | Loach feed added with porcine hemoglobin powder |
| CN104351466A (en) * | 2014-10-16 | 2015-02-18 | 浙江索纳克生物科技有限公司 | Low-ash porcine hemoglobin decolorization method |
| CN104399262A (en) * | 2014-10-16 | 2015-03-11 | 浙江索纳克生物科技有限公司 | Porcine blood protein powder drying and recycling apparatus |
| CN104431413A (en) * | 2014-10-16 | 2015-03-25 | 浙江索纳克生物科技有限公司 | Method of comprehensively utilizing pig blood |
| CN105795462A (en) * | 2016-03-29 | 2016-07-27 | 吉林省国鹿生物科技有限公司 | Preparation method of deer blood tablet polypeptide dry powder |
| CN105969817A (en) * | 2016-05-16 | 2016-09-28 | 金光 | Process for producing amino acid from blood of slaughtered livestock and poultry |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102125164B (en) * | 2010-10-29 | 2013-08-28 | 成都天屹生物科技有限公司 | Preparation method of pig blood fibrin polypeptide powder |
| CN102125164A (en) * | 2010-10-29 | 2011-07-20 | 成都天屹生物科技有限公司 | Preparation method of pig blood fibrin polypeptide powder |
| CN102599331A (en) * | 2011-01-20 | 2012-07-25 | 天津宝迪农业科技股份有限公司 | Preparation method for high-protein low-ash animal plasma protein powder |
| CN102488078A (en) * | 2011-11-15 | 2012-06-13 | 天津宝迪农业科技股份有限公司 | Preparation method for decolorized blood globulin powder |
| CN103039693B (en) * | 2013-01-09 | 2014-04-30 | 合肥工业大学 | Preparation method of modified livestock and poultry plasma protein powder |
| CN103039693A (en) * | 2013-01-09 | 2013-04-17 | 合肥工业大学 | Preparation method of modified livestock and poultry plasma protein powder |
| CN103275191A (en) * | 2013-06-19 | 2013-09-04 | 天津兰瑞生物技术有限公司 | Method for largely and quick extracting tachyplesin peptide |
| CN104171260A (en) * | 2013-07-31 | 2014-12-03 | 北京澳龙港生物技术研究中心 | Functional peptide protein powder, and preparation method and applications thereof |
| CN103431155A (en) * | 2013-09-16 | 2013-12-11 | 陆杰坤 | Production technology for enzymolysis and decoloration of spray-dried animal blood cells |
| CN103589770B (en) * | 2013-10-14 | 2015-12-23 | 四川农业大学 | Pig whole blood enzymolysis is low peptide and amino acid whose industrial process |
| CN103589770A (en) * | 2013-10-14 | 2014-02-19 | 四川农业大学 | Industrial production method of low peptides and amino acids through enzymolysis of pig whole blood |
| CN104211755A (en) * | 2014-08-21 | 2014-12-17 | 广州天赐福音生物科技有限公司 | Method for extracting biologically active peptides (BAPs) in livestock and poultry blood as well as BAPs and application thereof |
| CN104211755B (en) * | 2014-08-21 | 2017-12-19 | 肖应庆 | Biologically active peptide BAPs extracting method and biologically active peptide BAPs and application in a kind of animal blood |
| CN104256199A (en) * | 2014-10-16 | 2015-01-07 | 浙江索纳克生物科技有限公司 | Loach feed added with porcine hemoglobin powder |
| CN104431413A (en) * | 2014-10-16 | 2015-03-25 | 浙江索纳克生物科技有限公司 | Method of comprehensively utilizing pig blood |
| CN104399262A (en) * | 2014-10-16 | 2015-03-11 | 浙江索纳克生物科技有限公司 | Porcine blood protein powder drying and recycling apparatus |
| CN104351466A (en) * | 2014-10-16 | 2015-02-18 | 浙江索纳克生物科技有限公司 | Low-ash porcine hemoglobin decolorization method |
| CN105795462A (en) * | 2016-03-29 | 2016-07-27 | 吉林省国鹿生物科技有限公司 | Preparation method of deer blood tablet polypeptide dry powder |
| CN105969817A (en) * | 2016-05-16 | 2016-09-28 | 金光 | Process for producing amino acid from blood of slaughtered livestock and poultry |
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Application publication date: 20100707 |