CN101595848A - The artificial culturing method of a kind of Neptunea cumingi - Google Patents
The artificial culturing method of a kind of Neptunea cumingi Download PDFInfo
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- CN101595848A CN101595848A CNA2009100125326A CN200910012532A CN101595848A CN 101595848 A CN101595848 A CN 101595848A CN A2009100125326 A CNA2009100125326 A CN A2009100125326A CN 200910012532 A CN200910012532 A CN 200910012532A CN 101595848 A CN101595848 A CN 101595848A
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Abstract
The present invention relates to the artificial culturing method of a kind of Neptunea cumingi, to plant the spiral shell cylinder mould of packing into and carry out the marine site and store up, this cylinder mould is put 8 kind spiral shells for every layer, plants the spiral shell back of laying eggs and collects oopod and carry out indoor hatching and cultivate, when young spiral shell spiral shell shell in oopod has formed will hatch the time, vertically break oopod.Advantage is: by the present invention can provide a kind of can obtain a large amount of high-qualitys, healthy fertilized egg and and then cultivate a large amount of Neptunea cumingi seeds, can also support link temporarily at kind of spiral shell accelerating simultaneously and reduce seedling cost.
Description
Technical field
The present invention relates to a kind of conch artificial culturing method, the artificial culturing method of especially a kind of Neptunea cumingi.
Background technology
Neptunea cumingi (Neptunea arthritica comingii) belongs to Gastropoda, narrow tongue order, and Buccinidae, the large-scale kind of warm water, maximum ripe individuality can reach more than the 10cm.Neptunea cumingi is the distinctive conch kind of China, mainly is distributed in the yellow Bohai Sea of China, and with the highest, the optimal quality of Dalian Huanghai Sea output, shell is big and matter is thick.The conch kind that Neptunea cumingi is still rarer in the world, its meat flavour is bright fragrant, is of high nutritive value, and by people are liked food, therefore has very high market value.
The production of Neptunea cumingi is at present mainly adopted based on natural waters and is caught, along with the demand to Neptunea cumingi on the market is on the increase, natural waters adopt the demand that can't satisfy market of catching, therefore, be badly in need of cultivating, culturing and satisfy the wilderness demand in market by industrial artificial.But the research of Neptunea cumingi reproductive biology only just began to carry out in recent years, and the production of large-scale production Neptunea cumingi seed also lacks systematic research, and industrial artificial is cultivated Study on Technology and do not appeared in the newspapers both at home and abroad.
Generally, existing about the conch artificial breeding, temporary the supporting of accelerating of planting spiral shell is the mode that adopts indoor accelerating, need change water and bait throwing in, so, will inevitably increase seedling cost.
Summary of the invention
In view of this, the present invention is directed to the technical problem of scale Neptunea cumingi artificial breeding, provide a kind of and support the artificial culturing method that link reduces the Neptunea cumingi of seedling cost temporarily at kind of spiral shell accelerating.
For reaching above-mentioned purpose, the invention provides the artificial culturing method of a kind of Neptunea cumingi:
Neptunea cumingi kind spiral shell marine site is stored up and is laid eggs, collects oopod, the indoor hatching cultivation of oopod.
Described kind of spiral shell cylinder mould of packing into carries out the marine site and stores up, and this cylinder mould is put kind of spiral shell below 8 for every layer.
Described artificial culturing method is in the indoor hatching step of oopod, and young spiral shell spiral shell shell in oopod has formed in the time of will hatching, and vertically breaks oopod.
In the described incubation step, it is below 7500/square metre that young spiral shell is cultivated density.
Described young spiral shell is raised with flowing water.
Plant the spiral shell marine site and store up, the 15 layers of cylinder mould of packing into, interlamellar spacing is 10~12cm, and the cylinder mould diameter is 34cm, and mesh is 0.5mm;
Collect oopod, with oopod under the perching knife shovel, the atresia fish box of the long 50cm that packs into * wide 40cm * high 20cm moves into indoor hatching;
The indoor hatching of oopod, change the water secondary every day between the incubation period, and each half amount was fallen the pond once in 4~5 days;
Cultivate young spiral shell, every day, full dose was changed water once, every day blowing aeration.
Described artificial culturing method is in the indoor hatching step of oopod, and hatching density is below 200/square metre.
Described kind of spiral shell shell is tall and big in 8cm.
Described kind of spiral shell stored up in the unimpeded marine site of current.
Artificial culturing method by Neptunea cumingi of the present invention, can obtain a large amount of high-qualitys, healthy fertilized egg, the a large amount of high-quality Neptunea cumingi seed of acquisition that can be simple, stable again, while is according to bag characteristics of laying eggs of Neptunea cumingi, adopt a kind of marine mode of supporting accelerating of hanging, with the accelerating of kind of spiral shell, laying eggs moves to the nature marine site: the first, can reduce seedling cost effectively, also guarantee output of seedling.The second, method of operating is simple.Three, because at natural marine site accelerating, so can reduce kind of spiral shell greatly because the phenomenon of mortality appears in water quality deterioration.Four, young spiral shell spiral shell shell in oopod has formed in the time of will hatching, and oopod is vertically broken, and helps hatching of young spiral shell.Five, the young spiral shell of control is cultivated density, can prevent to cause between spiral shell and eat mutually because cultivation density is excessive.Six, adopt flowing water to raise, help keeping water quality.
Embodiment
Neptunea cumingi is the offshore shellfish, moves in the argillo arenaceous seabed more, and larva can live in the intertidal zone, perches substrate and is mainly shale silty sand and sandy silt matter mud, perches 10~70 meters of the depth of waters, and wherein 20~30 meters are comparatively concentrated; Neptunea warm water shellfish, 0~24 ℃ of comfort zone, 8~20 ℃ of optimum water temperatures; Neptunea cumingi inhabits the higher sea area of salinity, and the river mouth that salinity is lower does not generally have Neptunea cumingi, salinity excursion 30~35 ‰, and the suitableeest is 31~32.5 ‰; Neptunea cumingi is to be the carnivorous shellfish of main food with other shellfish.
The genital system of Neptunea cumingi:
Male: by spermary, ductulus efferens, sperm-storing bag, vas deferens, penis is formed, the spermary atrophy of sperm-storing bag mature period, sperm-storing bag expands, and inside is full of the milky seminal fluid; Penis is positioned at the head upper right side, and near right feeler, the flourishing likeness in form of penis bird head is vitals of differentiating male and female.
Female: by ovary, oviduct, receptaculum seminis, albuminous gland, oopod, oviporous orifice is formed.
Modes of reproduction: the ovum group of a turriform of the oopod of Neptunea cumingi formation bonded to each other, shape resembles the corncob that a fringe is sloughed the maize grain, the ovum group who discharges by female body in annual late May~early July attached to fixed objects such as reefs on, avette as the one piece of melon seeds of each fertilization, the outside random arrangement of tip.Ovum was grown in oopod about 60 days, just became the young spiral shell of 8~12mm, and shell is transparent, and profile is clear, just pipped after some days young spiral shells.
Artificial culturing method of the present invention, utilize the lay eggs characteristics of bag of Neptunea cumingi, will plant the spiral shell marine site and store up and lay eggs, support the purpose that link reduces seedling cost temporarily thereby reach at kind of spiral shell accelerating in conjunction with the indoor hatching of oopod, further specify the present invention below by embodiment, but be not construed as limiting the invention.
Embodiment 1
Use 40,000 pieces on kind of spiral shell, 69.4 ten thousand pieces on the young spiral shell of outbound.
One, store up in the marine site of planting spiral shell:
Kind of spiral shell selects shell tall and big in 8cm, and well-grown, profile are complete, and undamaged wild Neptunea cumingi carries out gonad maturity accelerating for 40,000 pieces.40,000 pieces of close spiral shells extensions are supported in the sea area, and the employing mesh diameter is 0.5mm, and the cylinder mould diameter is 34cm, and every interlamellar spacing is that 15 layers of scallop of 12cm are formed cage, puts 8 on kind of spiral shell for every layer, according to concrete conditions such as kind of a spiral shell quantity, breed scales, also can put about 7 on kind of spiral shell for every layer.In management process, in time remove dead individuality, prevent mutual infection.
Two, planting the spiral shell investigation follows the tracks of:
Follow the tracks of kind of a spiral shell mate and oviposit situation, regularly carry out the sexual gland investigation, grasp the development condition and the situation of laying eggs, in time determine the oopod acquisition time.
The egg-laying time of Neptunea cumingi is annual late May~early July, 15.0 ± 0.1 ℃ of the water temperatures of laying eggs, the ovum group who is discharged by female body is attached on the fixed objects such as reef, avette as the one piece of melon seeds of each fertilization, the outside random arrangement of tip forms oopod, the oopod of the firm output of Neptunea cumingi is milk yellow, the ovum group of a turriform of formation bonded to each other, and shape is sloughed the corncob of maize grain as a fringe.
Three, the collection of oopod:
Generally since laying eggs to early July at the beginning of 6 months, 16640 of common property oopods after the kind spiral shell of storing up when the marine site is laid eggs and finished, move to indoor concentrating with oopod and cultivate hatching.During collection oopod is shoveled down gently with perching knife, the white atresia fish box of the long 50cm that packs into, wide 40cm, high 20cm is fully rinsed well, moves into the hatching of indoor culture pond.
Four, the hatching of oopod:
Oopod is taken indoor hatching.The oopod hatching density is unsuitable excessive, and 200/square metre, or be lower than 200/square metre, water temperature is a natural temperature.Observe fertilized egg in the oopod during 17.2~20.4 ℃ of water temperatures, its growth course is slow, approximately needs 40~60d.
The growth course of table 1 Neptunea cumingi embryo in oopod
Time | Developmental stage | Water temperature ℃ | The oopod color | Individual big or small mm |
6 hours | Emit polar body | 17.6 | Yellow fraction | 0.3 |
32 hours | 4 cells | 17.6 | Yellow fraction | |
2 days | 8 cells | 17.9 | Yellow fraction | |
3 days | 36 cells | 18.2 | Yellow fraction | |
4~5 days | Blastaea | 18.6 | Yellow fraction | |
6~8 days | Primitive gut | 19.6 | Yellow fraction | 4 |
15~18 days | Veliger | 20.7 | Deepen slightly | 4.5 |
The growth course of table 2 Neptunea cumingi larva and young spiral shell
Time | Water temperature ℃ | Developmental stage | Size mm | The form characteristics |
About 15 days | 20.7 | Veliger | 4.5 | Two faces are arranged, cilium is arranged above, and the swing that does not stop. |
21.2 | Veliger | 5 | Differentiate foot and operculum under the circular face, become milk yellow, faucal forms gradually, Transparent color. | |
21.6 | Later stage veliger | 5~6 | Foot is further grown before and after the larva, forms wide flat Shu face, stretches out again and again. | |
25 days | 21.8 | Abnormal larva | 6~8 | Face is degenerated gradually, and head, eye form, and foot is flourishing, kiss can stretch out outside the shell. |
1.5~2 months | 22.0 | Young spiral shell | 10.0 | Internals such as outer embrane form, and gyration is not completed into, and shell is hard, color with become the spiral shell unanimity, rupture of membranes and going out. |
20.2 ℃ of young spiral shells of water temperature begin rupture of membranes and go out.The hatching larva hatches from the oopod at edge earlier in the cluster oopod, progressively extends to the centre then.The first seam of splitting from the tip of oopod in the hatching process, young then spiral shell gets out a part earlier, and faucal is partly stayed the outside, slowly climbs outward then.
Change the water secondary during this time every day, each half amount.The pond fell in 4~5 days once.Will be in the hatching later stage at the timely young spiral shell of collection of each when falling the pond, formed will hatch the time at young spiral shell spiral shell shell in oopod, oopod vertically be broken, help hatching of young spiral shell.
Five, young spiral shell is cultivated:
The collection of young spiral shell: altogether in five batches, the young spiral shell young that rupture of membranes is gone out is collected in the cement pit and cultivates.
Cultivate density: the 5000/m of young spiral shell that has just hatched
2Forbid density excessive, cause between spiral shell and eat mutually.
Indoor pond incubation rate:
Indoor pond incubation rate=young spiral shell number/total ripe number of ovum grain * 100%
=78.3/115×100%=68.1%
Water quality: the young is put into the cement pond that bottom is covered with corrugated plating, 22.6 ℃ of water temperatures, every day, full dose was changed water once (nature seawater), preferably flowing water is raised, and helps keeping water quality, every day blowing aeration.
The condition of water quality that the young spiral shell of table 3 is cultivated
The time sequence number | Temperature | Salinity | Dissolved oxygen | The dissolved oxygen degree of saturation |
1 | 22.6 | 30.01 | 6.80 | 88% |
2 | 20.4 | 30.18 | 6.02 | 80% |
3 | 16.8 | 30.10 | 6.30 | 83% |
Fall the pond: along with the growth of young spiral shell, foul residual in the pond is many, is attached with the mucus of a large amount of young spiral shells secretions at the bottom of pool wall and the pond, can cause the deterioration of water quality, and the growth of young spiral shell is had certain influence.The pond fell in 3~5 days once, and in when falling the pond, noted gently taking, and puts down gently.
Bait throwing in: about 2 days of young spiral shell hatching back, stomach forms by the time, begins bait throwing in every day at dusk, and can throw early stage such as some vegetal baits such as benthic diatom etc., along with growing of young spiral shell throw something and feed gradually carnivorous bait such as mussel, crab, scallop body, low-grade fish.To handle clean before scallop body and mussel are thrown something and fed, young spiral shell has gregarious life habit, feeding volume is decided on the situation of ingesting of young spiral shell, but feeding volume will be greatly and is guaranteed that evenly most of young spiral shells ingest, along with the growth of young spiral shell increases daily ration, feeding quantity gradually, remaining bait will be cleaned out and prevent polluted water, influences the growth of young spiral shell, regularly fall pond and inhaling at the end, with at the bottom of remaining residual bait and the pond, the foul of pool wall and dead young spiral shell remove.
Daily management: dailyly also will check inflation situation and water valve, young spiral shell has the habit of creeping, and every day, the young spiral shell that will climb to pool wall and outside was regularly collected in the pond, prevents that long-time doing from revealing and dead.Throw something and feed, change water, when falling the pond and note gently taking, put down gently, and will leave a spot of water in the pond during mobile adherance, alleviate the weight of adherance, thereby alleviate damage dynamics young spiral shell.
The growing state of the young spiral shell of table 4
Growth along with young spiral shell, foul residual in the pond is many, adherances etc. also are attached with a large amount of mucus, growth to young spiral shell has certain influence, and the growth rate of young spiral shell is inconsistent simultaneously, and the situation of ingesting is variant, in order to create better living environment, it has been carried out pond, screening, can distinguish by specification during screening, batch mixed.
The screening situation statistical form of the young spiral shell of table 5
Six, young spiral shell outbound
Young spiral shell is through the cultivation of 2 wheat harvesting periods, the growing fine of young spiral shell, and during outbound, the population mean specification is 1.59cm, total quantity is that 69.4 ten thousand pieces of concrete quantity see the following form:
Table 6 outbound specification quantity
Embodiment 2
Utilize identical breeding method, use 270,000 pieces on kind of spiral shell, bring out 200.2 ten thousand pieces on young spiral shell.
One, plants the sexual gland investigation of spiral shell
Follow the tracks of kind of a spiral shell mate and oviposit situation, regularly carry out the sexual gland investigation, the per 7 days kind spiral shells to self-sow in the marine site carry out the gonad development situation and measure.Concrete grammar is to get 20 at every turn, and each 10 of male and female are measured the gonad index.In view of the heavy percentage of liveweight large percentage of neptune shell, obvious in order to make numerical value change, heavily account for the heavy percentage of soft body as the gonad index with sexual gland.To plant spiral shell and boil 10 minutes, take out soft body, drain well, separation is weighed, and simultaneously sexual gland is carried out sections observation.
Table 7 sexual gland index and time relation
Time sequencing | Sexual gland heavy (g) | Soft body heavy (g) | Sexual gland index (%) |
1 | 57.7±20.8 | 2.31±0.76 | 3.63±0.96 |
2 | 66.3±11.2 | 2.52±0.39 | 3.71±0.77 |
3 | 56.9±7.9 | 2.15±0.46 | 3.82±0.64 |
4 | 63.4±13.5 | 6.67±1.26 | 10.5±1.34 |
5 | 71.2±21.6 | 12.5±3.45 | 17.6±2.12 |
6 | 68.8±15.6 | 15.9±4.23 | 19.8±1.59 |
7 | 67.9±16.9 | 14.3±2.31 | 21.1±2.56 |
8 | 58.6±15.4 | 12.4±1.97 | 21.2±3.76 |
As can be seen from Table 7, after the sexual gland index reaches the peak period, be in mild state, show to close on and lay eggs.
Two, plant spiral shell quantity and put situation in a suitable place to breed
Adopt and catch kind of a spiral shell quantity 270,000, the use of kind spiral shell is formed the cage extension and is supported in buoyant raft district (table 8).Generally form cage and select 15 layers for use, plant spiral shell and put 8 for every layer.Natural waters kind spiral shell is not is not thrown something and fed, and regularly its developmental state is carried out investigation records.
Table 8 kind of spiral shell is hung and is supported the situation distribution table
Project | Place 1 | Place 2 | Place 3 | Place 4 | Place 5 |
Plant spiral shell quantity (ten thousand) | 3.8 | 8.4 | 6.9 | 4.2 | 3.5 |
Three, lay eggs and hatch
Natural waters is hung to support and is hatched after kind of a spiral shell is laid eggs, and does not need any bait of throwing something and feeding in the hatching process.In order to accelerate hatching speed and to improve incubation rate, when the young spiral shell in oopod is about to maturation, the whole strip ofves of oopod to increase permeability, can be increased the contact area of each ovum grain and nature seawater.In the hatching process every day regularly, dissect surely with batch getting egg capsule, observe embryonic development.
The embryonic development situation of table 9 Neptunea cumingi
Time | Developmental stage | Water temperature (℃) | The oopod color | Individual size (mm) |
6 hours | Emit polar body | 17.6±0.3 | Yellow fraction | 0.3±0.02 |
32 hours | 4 cells | 17.7±0.4 | Yellow fraction | \ |
2 days | 8 cells | 17.9±0.5 | Yellow fraction | \ |
3 days | 32 cells | 18.1±0.3 | Yellow fraction | \ |
4-5 days | Blastula stage | 18.5±0.7 | Yellow fraction | \ |
6-8 days | Gastrul stage | 18.9±0.8 | Deepen | \ |
15-17 days | Veliger | 20.6±0.9 | Deepen | 4±0.25 |
The situation statistical form is laid eggs, hatched to table 10 various places point Neptunea cumingi
Project | Place 1 | Place 2 | Place 3 | Place 4 | Place 5 |
Plant spiral shell quantity (ten thousand) | 3.8 | 8.4 | 6.9 | 4.2 | 3.5 |
Oopod number (ten thousand) | 0.97 | 2.7 | 3.6 | 1.02 | 1.22 |
Spawning rate (%) | 51.1 | 64.3 | 104.3 | 61.8 | 69.7 |
Produce young number (ten thousand) | 14 | 87 | 63 | 20 | 26 |
Each oopod produce young number (piece) | 14.4 | 32.2 | 17.5 | 19.6 | 21.3 |
Annotate: the computing formula of spawning rate is: oopod number/(planting spiral shell quantity * 1/2), wherein female-male proportion calculated with 1: 1.
As can be seen from Table 10, the spawning rate in place 3 surpasses 100%, and wherein female body is too much, and other each place spawning rate differences are not remarkable; In addition, in cultivating process of growth, contrast kind of spiral shell sea, these two kinds of methods of indoor accelerating, marine kind spiral shell egg-laying time late 3~5 days than the egg-laying time of indoor kind of spiral shell, but marine spawning rate and indoor kind of spiral shell of planting spiral shell differs endlessly.
Four, the cultivation of young spiral shell
Young spiral shell indoor culture, every bait throwing in 5 days once, daily ration, feeding quantity is according to the situation control of ingesting, and bait is based on mussel.Every day, full dose was changed water once, in time removed residual bait, prevented to ruin water quality, influenced the long speed of young spirodromy, cultivated 7500/square metre of density, threw hollow brick as adherance, continuous charge, and flowing water is raised, and regularly falls about 10~15 days of pond once.Daily running check inflation situation and the water valve wanted, young spiral shell has the habit of swashing, and the young spiral shell that will climb to pool wall and outside was regularly collected and was prevented in the pond that long-time doing from revealing and dead every day.Throw something and feed, change water, when falling pond adherance and note gently taking, put down gently, and will leave a spot of water in the pond when mobile, alleviating the weight of adherance, thereby alleviate damage dynamics young spiral shell.
The cultivation of plunging into the commercial sea of young spiral shell, the specification of plunging into the commercial sea 1.45 ± 0.5cm, 20.6 ℃ of the water temperatures of plunging into the commercial sea, the young spiral shell of part every bait throwing in 5 days once, based on scallop edge, the not bait throwing in of the young spiral shell of part, routine observation development condition; Through observing as can be known, the long situation of the young spirodromy of bait throwing in is far superior to the young spiral shell of not bait throwing in, and visible an amount of bait throwing in has tangible influence to young spirodromy length; On body weight, bait throwing in is about 50 days, the young spiral shell average weight gain 0.79g that throws something and feeds, and the balanced growth 0.08g that does not does not throw something and feed, and the long speed of the young spirodromy of as seen not throwing something and feeding is extremely slow.
Based on above explanation, implement the artificial culturing method of Neptunea cumingi of the present invention, have the following advantages:
1, plants the preceding male and female of distinguishing of spiral shell accelerating, can increase kind of the spiral shell quantity of effectively laying eggs;
2, marine spawning rate of planting spiral shell and indoor kind of spiral shell does not have too big difference, and the sea is planted the spawning rate of spiral shell even can be higher than indoor kind of spiral shell;
3, regularly collect the young spiral shell that hatches, the foul at the bottom of the cleaning pond prevents at the bottom of the hatchery dirtyly excessively, makes young spiral shell death, and the residue oopod will fall the pond, and preferably flowing water hatching prevents that environment of hatching from worsening;
4, indoor young spiral shell of supporting the temporarily mussel of throwing something and feeding is comparatively desirable, not only young spiral shell ingest effective, and low to the pollution level of water quality;
5, input corrugated plating effect is better, helps young spirodromy and lives and ingest;
6, the survival rate of young spiral shell is higher, reaches 84.3~93.8%.
The above is preferred embodiment of the present invention only, is not to be used to limit protection scope of the present invention.
Claims (9)
1, the artificial culturing method of a kind of Neptunea cumingi is characterized in that, Neptunea cumingi kind spiral shell marine site is stored up and laid eggs, collects oopod, the indoor hatching cultivation of oopod.
2, the artificial culturing method of a kind of Neptunea cumingi according to claim 1 is characterized in that, described kind of spiral shell cylinder mould of packing into carries out the marine site and stores up, and this cylinder mould is put kind of spiral shell below 8 for every layer.
3, the artificial culturing method of a kind of Neptunea cumingi according to claim 2 is characterized in that, described artificial culturing method is in the indoor hatching step of oopod, and young spiral shell spiral shell shell in oopod has formed in the time of will hatching, and vertically breaks oopod.
4, the artificial culturing method of a kind of Neptunea cumingi according to claim 3 is characterized in that, in the described incubation step, it is below 7500/square metre that young spiral shell is cultivated density.
5, the artificial culturing method of a kind of Neptunea cumingi according to claim 4 is characterized in that, described young spiral shell is raised with flowing water.
6, according to the artificial culturing method of the described a kind of Neptunea cumingi of arbitrary claim in the claim 1 to 5, it is characterized in that,
Plant the spiral shell marine site and store up, the 15 layers of cylinder mould of packing into, interlamellar spacing is 10~12cm, and the cylinder mould diameter is 34cm, and mesh is 0.5mm;
Collect oopod, with oopod under the perching knife shovel, the atresia fish box of the long 50cm that packs into * wide 40cm * high 20cm moves into indoor hatching;
The indoor hatching of oopod, change the water secondary every day between the incubation period, and each half amount was fallen the pond once in 4~5 days;
Cultivate young spiral shell, every day, full dose was changed water once, every day blowing aeration.
7, the artificial culturing method of a kind of Neptunea cumingi according to claim 2 is characterized in that, described artificial culturing method is in the indoor hatching step of oopod, and hatching density is below 200/square metre.
8, the artificial culturing method of a kind of Neptunea cumingi according to claim 1 and 2 is characterized in that, described kind of spiral shell shell is tall and big in 8cm.
9, the artificial culturing method of a kind of Neptunea cumingi according to claim 8 is characterized in that, described kind of spiral shell stored up in the unimpeded marine site of current.
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CN107960352A (en) * | 2017-12-07 | 2018-04-27 | 广东海洋大学 | The breeding method of young spiral shell in a kind of Trumpet shell whelk artificial breeding |
CN107960352B (en) * | 2017-12-07 | 2021-08-27 | 广东海洋大学 | Method for cultivating young hemifusus tuba in artificial breeding of hemifusus tuba |
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