CN101011432B - Use of extract of bracken flavone as medicament for lead expelling and lead poisioning alleviation - Google Patents
Use of extract of bracken flavone as medicament for lead expelling and lead poisioning alleviation Download PDFInfo
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- CN101011432B CN101011432B CN2007100194717A CN200710019471A CN101011432B CN 101011432 B CN101011432 B CN 101011432B CN 2007100194717 A CN2007100194717 A CN 2007100194717A CN 200710019471 A CN200710019471 A CN 200710019471A CN 101011432 B CN101011432 B CN 101011432B
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- lead
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- flavone
- bracken
- bracken flavone
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Abstract
The invention relates to a method for using bracken chromocor extractive to prepare the drug used to discharge lead and release the lead poison, wherein, each day and each kilogram can use 30-180mg drug, to obtain significant lead discharge effect. And each day and each kilogram can use 50-300mg bracken chromocor to obtain significant lead poison release effect. The inventive drug will not discharge needed element of human body, while the bracken chromocor extractive contains the needed elements as iron and zinc of people.
Description
Technical field
The present invention relates to the purposes of extract of bracken flavone.
Background technology
Lead is poisonous metal, no any physiological action in human body, and ideal blood lead concentration should be zero.But along with the fast development of modern industry and Modern Traffic industry, the environment lead contamination is on the rise, and health in serious harm.Lead is a kind of pewter metal, belong to nondegradable environmental contaminants, in environment, can accumulate for a long time, can pass through per os, respiratory tract, gastrointestinal tract and skin absorbs lead such as water, air, food, part was accumulated and can not be drained fully in the human body midium or long term, cause the infringement of systems such as human nerve, hemopoietic, immunity, digestion and each organ aspect of human body to some extent, and cause chronic poisoning.Compare with the adult, the child is to the special susceptibility of being absorbed with of lead, brain functioies such as major injury child's cognition and neurobehavioral, and this infringement can not Zheng change, and influences growth of children.
For solving saturnine long-term prevention and treatment problem, people have carried out a large amount of research to Plumbum removing mechanism and method, have developed Pb excluding health care product and medicine.On Plumbum removing mechanism research at present mainly contain following Plumbum removing mechanism: 1. complexation mechanism: comprise complexation mechanism, vitamin C and the plumbous complexations etc. such as complexation mechanism, konjaku powder of natural polymers such as wide spectrum complexing, protein, amino-acid complex mechanism, pectin; 2. antagonism mechanism: the antagonism mechanism, vitamin C and the B that comprise elements such as calcium, ferrum, zinc
1Deng antagonism mechanism, garlicin antagonism mechanism etc.
Nowadays people study and promote the lead-expelling function factor and functional mass that following a few class will be arranged:
1. EDTA; 2. tea polyphenols; 3. vitamin C and vitamin B
14. selenium; 5. L-methionine; 6. potassium iodide; 7. garlicin; 8. konjaku powder; 9. pectin etc.
At present, the market Pb excluding health care product has thousand fruit flowers to fall plumbous health care liquid, Receptaculum Helianthi low methoxyl pectin, strengthen SOD Sucus Rosae Normalis etc.But these products one are that kind is few, the 2nd, and product is not mature enough, and the Plumbum removing effect is undesirable.Chang Yong treatment lead poisoning medicine has calcium disodium chelate (CaNa clinically
2EDTA), calcium calcium trisodium pentetate and zinc calcium trisodium pentetate, sodium dimercaptosuccinate, dimercaptosuccinic acid, penicillamine, dimercaptopropanol, BAL, dimercaptosuccinic acid etc.These medicines have also been discharged trace element essential in the body in plumbous having discharged, and cause malnutrition, and significant side effects is arranged.For this reason, the assistant officer wait to develop that side effect is little, taking convenience, cost-effective control lead poisoning medicine and anti-plumbous health product, lead-expelling function food etc.
Summary of the invention
The present invention by a series of extract of bracken flavone of carrying out Plumbum removing and alleviate the lead poisoning zoopery, and observe it in Plumbum removing with alleviate the lead poisoning effect, aim to provide that a kind of side effect is little, taking convenience, cost-effective extract of bracken flavone are as Plumbum removing and alleviate application in the lead poisoning medicine.
Herba Fimbristylis dichotomae another name fist dish, the dish of complying with one's wishes, Herba Fimbristylis dichotomae etc.It is the young tender leaf that picks up from the Herba pteridii latiusculi [Pteridiumaquilinum (L.) Kuhn var.latiusculum (Desv.) Underw] in the Pteridiaceae Cyclosorus, because of it is grown in the mountain forest wild country, pollute for a short time, be rich in multiple nutritional components, become the delicacy from mountain wild herbs that people like the human body beneficial.There are some researches show, be rich in flavone compound in the Herba Fimbristylis dichotomae, and proof bracken flavone compounds is in the external effect that has antioxidation and remove free radical.Have not yet to see the product and the Technology of Herba Fimbristylis dichotomae Plumbum removing food (health product, medicine), more extract of bracken flavone is not developed to Plumbum removing or separates Related product and technology such as saturnine food (health product), medicine.
The acquisition of extract of bracken flavone:
(1) acquisition of extract of bracken flavone: dry by the fire to 6-8 one-tenth fresh fiddlehead dried, after 103 ℃ of oven dry, be ground into powder again, slough Herba Fimbristylis dichotomae dry powder fat and remove fat-soluble pigment with ether, when the ether extract is colourless, wave ether in the most Herba Fimbristylis dichotomae powder, Herba Fimbristylis dichotomae dry powder is placed on to add entry or the fractional ethanol of different volumes or the fractional acetone of different volumes in the flask be that solvent refluxing extracts bracken flavone.Each about 2-3h of lixiviate, extraction temperature is 50 ℃-90 ℃, common lixiviate 3-5 time, the lixiviate solid-to-liquid ratio is 1:5-10 (g:ml), merges all lixiviating solution and is condensed into pulpous state or with 103 ℃ of oven dry of slurry in rotary evaporator, pulverize the crude flavonoid powder powder.
(2) flavones content is measured in the extract of bracken flavone: adopt the spectrophotometry flavones content, promptly utilize flavone compound and aluminum salt to generate red complex, with the rutin is standard substance, measure absorbance at the 510nm place, the gained data can obtain the regression equation of standard curve through regression treatment.Get after the certain volume sample liquid records absorbance by standard curve same procedure operation, go out flavones content by regression equation calculation.
Flavones content in the extract of bracken flavone among the present invention; Extract of bracken flavone is as being slurry, and the mass fraction that flavone accounts for slurry is 10-15; Extract of bracken flavone is as being crude flavonoid powder powder (Powdered thing), and the mass fraction that flavone accounts for the crude flavonoid powder powder is 15-30.
The extract of bracken flavone pharmacological action is strong, compares with alleviation lead poisoning medicine with traditional Plumbum removing, and safety non-toxic, side effect is little, and taking convenience has good prospect in medicine.
To clinical unleaded poisoning symptom performance, but what lead content was higher in the body carries out Plumbum removing with extract of bracken flavone: the extract of bracken flavone by edible various dose makes the lead tolerance in whole blood, the liver significantly reduce, compare the lead tolerance reduction with the contrast that does not eat extract of bracken flavone and reach statistical significant level, thereby reach the purpose of Plumbum removing.
Clinical manifestation is had alleviating or treat with the bracken flavone compounds of lead poisoning symptom: on the whole, lead poisoning influences growth promoter; On the biochemistry level, one of saturnine important behaviour is exactly the active significantly reduction of SOD in the tissue (liver, blood etc.), and level of lipid peroxidation is that malonaldehyde (MDA) content significantly raises, and body cell sustains damage.The lead poisoning person is by the extract of bracken flavone of edible doses; make the growth promoter of lead poisoning body be restored to a certain extent; especially the SOD activity is restored; reach the SOD activity level of normal body; compare with the lead poisoning person SOD that does not eat extract of bracken flavone, difference reaches significant level.While lead poisoning person makes that by the extract of bracken flavone of edible doses the lipid peroxide degree in the tissue is compared remarkable reduction with the lead poisoning person who does not eat extract of bracken flavone, that is: make the MDA content in the tissue significantly reduce.
In sum, the extract of bracken flavone of the edible doses of lead poisoning person is eased lead poisoning or treats, lead poisoning and the body of damaged can obtain recovery to a certain degree, or even recover preferably.
The application of extract of bracken flavone aspect Plumbum removing: use the amount of bracken flavone 30-180mg (use amount of extract of bracken flavone is converted with the flavones content that determines) to eat every day by per kilogram of body weight, can reach significant Plumbum removing effect.Concrete using method:
Calculate the amount of using extract of bracken flavone every day by 30-180mg flavone/kg.bw, take by weighing pulpous state or Powdered extract of bracken flavone by this amount, add 1-5 doubly the warm water more than 50 ℃ or the abundant stirring and dissolving 5-10 of boiling water minute, cold back is food (drink) usefulness directly, once a day.
Illustrate: every day, the use amount of extract of bracken flavone was determined to abide by following principle: child's use amount can be on the low side, and the teenager use amount is medium, and adult's use amount can be higher.
Extract of bracken flavone is being alleviated or treated in the lead poisoning and use: the use amount by per kilogram of body weight use every day bracken flavone 50-300mg (use amount of extract of bracken flavone is to convert to measure the flavones content that comes out) can reach significant alleviation lead poisoning or treat saturnine effect.Using method: calculate the amount of using extract of bracken flavone every day by 50-300mg flavone/kg.bw.Take by weighing pulpous state or Powdered extract of bracken flavone by this amount, add 1-5 doubly the warm water more than 50 ℃ or the abundant stirring and dissolving 5-10 of boiling water minute, cold back is food (drink) usefulness directly, once a day.
Illustrate: every day, the use amount of extract of bracken flavone was determined to abide by following principle: child or poisoning symptom the lighter's use amount can be on the low side, and teenager and adult or poisoning symptom weight person use amount can be higher.
The present invention has the following advantages:
1, the extract of bracken flavone system of the present invention's use extracts acquisition from natural in the wild vegetable-Herba Fimbristylis dichotomae that is loved by the people, and belongs to natural extract, and toxic and side effects is little, and is safe.
2, flavone compound has been proved to be abilities such as having complexing power and antioxidation, therefore Plumbum removing has complexation Plumbum removing and antagonism Plumbum removing double mechanism, not only can in Plumbum removing, not get rid of external by the relevant element needed by human yet, can use extract of bracken flavone to replenish an amount of useful element because of relevant elements such as the ferrum that itself contains needed by human in the extract of bracken flavone, zinc on the contrary to human body.
3, extract of bracken flavone easily obtains, and free from extraneous odour is suitable for different crowd, and is easy to use.Extract of bracken flavone also is easy to further be processed into tablet, powder, electuary, capsule etc. simultaneously, and good prospect in medicine is arranged.
The specific embodiment
In order to understand essence of the present invention better, the new purposes of extract of bracken flavone in pharmaceutical field is described below by concrete experimental result and data.
(1) experimental basis of extract of bracken flavone aspect Plumbum removing:
1 materials and methods
1.1 animal and grouping
50 of Kunming mouses, male, the about 20g of body weight.The quality certification number: real moving accurate No. 01 of Anhui doctor.Provide by Medical University Of Anhui's Experimental Animal Center.Animal is divided into 5 groups at random: the blank group; Model control group; Low dose group (the 30mg/kg.bw bracken flavone group of feeding day); Median dose group (the 90mg/kg.bw bracken flavone group of feeding day); High dose group (the 180mg/kg.bw bracken flavone group of feeding day).
1.2 the extraction of Herba Fimbristylis dichotomae crude flavonoid powder and flavones content are measured
Fresh fiddlehead dries by the fire to 6-8 and becomes dried, is cut into broken section of 2-3cm then, is ground into powder after 103 ℃ of oven dry again.The Herba Fimbristylis dichotomae dry powder that takes by weighing certain mass is with ether defatting and except that fat-soluble pigment, when the ether extract is colourless, wave ether in the most Herba Fimbristylis dichotomae powder, Herba Fimbristylis dichotomae dry powder is placed on adds entry in the flask or volume fraction is that 30%-80% ethanol or volume fraction are solvent for 30%-80% acetone, the reflux, extract, bracken flavone.Each about 2-3h of lixiviate, extraction temperature is 50-90 ℃, common lixiviate 3-5 time, the lixiviate solid-to-liquid ratio is 1:5-10 (g:ml), merges all lixiviating solution and is condensed into pulpous state or 103 ℃ of oven dry in rotary evaporator, pulverize the crude flavonoid powder powder.Adopting the spectrophotometry flavones content, promptly utilize flavone compound and aluminum salt to generate red complex, is standard substance with the rutin, measures absorbance at the 510nm place, and the gained data can obtain the regression equation of standard curve through regression treatment.Get after the certain volume sample liquid records absorbance by standard curve same procedure operation, go out flavones content by regression equation calculation.
1.3 animal and grouping
Adopt the plumbous animal model of preventative height.Except that the blank group is freely drunk the deionized water, all the other 4 groups equal freely drinking contain 1.0g/L acetate trihydrate lead [Pb (CH
3COO)
2.3H
2O] deionized water solution (Pb
2+Mass concentration is 546.2mg/L), change liquid weekly 2 times.Basic, normal, high dosage group gives animal subject and irritates stomach respectively by above-mentioned dosage setting, and blank group and model control group are irritated stomach and given the equal-volume deionized water, continuously 30d.After last was irritated stomach 24h, the broken end blood sampling was got the 1.0g blood sample and is put to death mice, gets liver 1.0g, with concentrated nitric acid and perchloric acid (both volume ratio 3:1) hot digestion, measures blood lead, liver lead content with graphite furnace atomic absorption spectrometry.
1.4 data statistics
Data represent that with X+S Spss10.0 software takes statistics to learn and handles, and group difference adopts the t check to analyze.
1.5 the result judges
Under the prerequisite that animal model is set up, experimental group and model control group relatively organize lead content obviously to descend, and handle by statistics, and significant difference is arranged, and the zoopery result of this given the test agent of decidable is positive.
2 result of the tests
2.1 influence to the mice body weight
Extract of bracken flavone sees Table 1 to the influence of mice body weight, the result show between each dosage group and the matched group initial body weight, whole opisthosoma heavy between there was no significant difference (P〉0.05).
Table 1 extract of bracken flavone to the influence of mice body weight (X+s, n=10)
2.2 organize plumbous measurement result
The measurement result of blood lead regulating liver-QI lead sees Table 2.The blood lead, the liver lead content that show 3 dosage groups all significantly are lower than model control group (p＜0.01).
The plumbous measurement result of table 2 blood lead regulating liver-QI (X+s, n=10)
* compares p＜0.01 with the type matched group of touching
2.3 animal experiment conclusion
Per os can significantly reduce a mice blood lead, liver lead content for extract of bracken flavone, and extract of bracken flavone has the promotion lead-expelling function to animal.
(2) experimental basis of extract of bracken flavone aspect alleviation or treatment lead poisoning;
1 materials and methods
1.1 animal and grouping
50 of Kunming mouses, regular grade, male, body weight 18 ~ 20g.The quality certification number: real moving accurate No. 01 of Anhui doctor.Provide by Medical University Of Anhui's Experimental Animal Center.Animal is divided into 5 groups at random: the blank group; Lead poisoning matched group (hereinafter to be referred as the Pb group); The lead poisoning condition 50mg/kg.bw bracken flavone group (hereinafter to be referred as Pb+50 group) of feeding in following day; The lead poisoning condition 150mg/kg.bw bracken flavone group (hereinafter to be referred as Pb+150 group) of feeding in following day, the lead poisoning condition 300mg/kg.bw bracken flavone group (organizing) of feeding in following day hereinafter to be referred as Pb+300.The lead poisoning condition is all the lead of the 50mg/kg.bw that feeds day.
1.2 the extraction of Herba Fimbristylis dichotomae crude flavonoid powder and flavones content are measured
Identical with narration method in the preamble " experimental basis of (one) extract of bracken flavone aspect Plumbum removing ".This no longer repeats.
1.3 prepare the bracken flavone aqueous solution and the lead acetate solution of three kinds of variable concentrations
5mg/ml, 15mg/ml, 30mg/ml bracken flavone aqueous solution are prepared in calculating respectively according to the flavones content in the crude flavonoid powder powder, in 4 ℃ of freezer storages.
Preparation Pb
2+Concentration is 1mg/ml lead acetate solution.
1.4 feeding method
By every mice average weight 20g, add lead acetate solution 1ml every day in 0.5 gram feedstuff, drying, and the lead of the 50mg/kg.bw that is equivalent to feed day is enough to cause lead poisoning; By every mice average weight 20g, get 0.5 gram feedstuff every day more equally, in this feedstuff, add the corresponding flavone aqueous solution 0.2ml (being equivalent to feed day 50mg/kg.bw, 150mg/kg.bw, 300mg/kg.bw bracken flavone) of 1.3 preparations, drying.By these two kinds of feedstuffs of the abundant mixing of experimental design mice (every night about 8 o'clock) of feeding, give common mixed feed about 8 o'clock on the secondth.Freely drink water (boiling tap water cooling).
1.5 MDA content and the active mensuration of SOD in MDA content and the hepatic tissue in the serum.
After feeding continuously 14, the mice sacrificed by decapitation is got blood, blood is isolated serum in the centrifugal 10min of 4000r/min, deposits in 4 ℃ of refrigerators to be measured; Separate liver rapidly, (0.05mol/L, pH7.8), 12000r/min homogenate 3min, homogenate get supernatant and deposit in 4 ℃ of refrigerators to be measured in the centrifugal 15min of 10000r/min with 1:20 (m/v) ratio adding phosphate buffer to get mouse liver.
MDA measures the thiobarbituricacid method (TBA method) that adopts, and measures absorbance (O.D value) in 532nm wavelength place.The TBA method is based on unsaturated fatty acid and passes through radical reaction, produce peroxylradicals, and then oxidation generates epoxide, the latter generates malonaldehyde (MDA), the 1mol malonaldehyde can generate three methines (trimethine) of rufous with the 2molTBA effect, and three methines have maximum absorption band at the 532nm place.Therefore, how much just being reflected on the absorbance size at 532nm place of mda content is the sign of weighing free radical reaction course.
The active mensuration of SOD adopts the pyrogallol autoxidation method of improvement, measures absorbance (O.D value) in 325nm wavelength place.Calculate the SOD vigor.Unit of enzyme activity is defined as in every milliliter of reactant liquor, and per minute suppresses pyrogallol autoxidation speed and reaches 50% pheron amount.
1.6 data statistics
Data represent that with X+S Spss10.0 software takes statistics to learn and handles, and group difference adopts the t check to analyze.
2 results and analysis
2.1 the variation of mice body weight
Feed continuously and weighed in 14th, record mice body weight such as table 1.The result shows that the body weight of lead poisoning group mice significantly is lower than blank group (P＜0.01), illustrates that lead poisoning causes the mice growth to be suppressed.The lead poisoning bracken flavone group body weight of feeding simultaneously increases to some extent.When the amount of adding bracken flavone reaches 300mg/kg.bw, compare mice weight increase significantly (P＜0.05) with the lead poisoning group.Relatively Pb+300 group and blank group mice body weight, finding does not have significant difference, illustrate that the bracken flavone of feeding has certain repair to mice physical growth under the lead poisoning, and with the increase of the bracken flavone amount of feeding, the reinforcement of mice physical growth recovery capability.
Table 1. is fed bracken flavone and lead poisoning to the influence of mice body weight (X ± s)
Compare * P＜0.05**P＜0.01 with the Pb processed group
2.2 SOD activity in the murine liver tissue
The SOD activity is as shown in table 2 in the murine liver tissue.Under the lead poisoning, active significantly reduce (P＜0.01) of SOD, this may be because the lead acetate of feeding continuously causes the ability of the synthetic SOD enzyme of mice poisoning back hepatic tissue to reduce and the activity of SOD enzyme is suppressed.The independent lead poisoning group of Pb+50 group SOD specific activity is slightly high, when the bracken flavone amount of feeding is 150mg/kg.bw, active rise obviously (P＜0.05) of SOD, when the bracken flavone amount of feeding reaches 300mg/kg.bw, active rise extremely significantly (P＜0.01) of SOD does not relatively have significant difference with the blank group, reaches normal level, illustrate that the bracken flavone of feeding can repair the infringement of the cellular oxidation that lead poisoning causes, and the ability of the synthetic SOD of body is recovered.
Table 2 is fed bracken flavone and lead poisoning to the active influence of SOD in the murine liver tissue (X ± s)
Compare * P＜0.05 * * P＜0.01 with the Pb processed group
2.3 the relative amount of MDA in the murine liver tissue
The relative amount of MDA is as shown in table 3 in the murine liver tissue.The O.D value of MDA enlarges markedly (P＜0.01) than blank group in the Pb group murine liver tissue.The Pb+50 group is compared with independent lead poisoning group, the O.D value descends significantly (P＜0.05), and the Pb+150 group is compared with independent lead poisoning group, and the decline of O.D value is (P＜0.01) extremely significantly, the Pb+300 group is compared with independent lead poisoning group, and the decline of hepatic tissue O.D value is (P＜0.01) extremely significantly.Illustrate the bracken flavone of feeding to reduce mice body endogenous cause of ill lead poisoning and a large amount of MDA content that produces, reduce level of lipid peroxidation.
Table 3. is fed bracken flavone and lead poisoning to the influence of MDA content in the murine liver tissue (X ± s)
Compare * P＜0.05 * * P＜0.01 with the Pb processed group
Relatively Pb+50 group and Pb+150 group show that latter MDA content fall is big, and the two difference reaches significantly extremely level (P＜0.01), and the Pb+300 group is compared with the Pb+150 group, the MDA content of Pb+300 group further descends, and the two difference reaches utmost point significant level (P＜0.01), as shown in Figure 1.The feed decline of MDA content in the amount of bracken flavone and the murine liver tissue of prompting is to a certain degree positive correlation.Illustrate that the bracken flavone amount of feeding is big more, reduce the ability of organizing level of lipid peroxidation that causes because of lead poisoning and strengthen.But relatively Pb+300 group and blank group find that Pb+300 group MDA content still is significantly higher than blank group (P＜0.01), see Fig. 1, illustrates that Pb+300 organizes not make that yet MDA content is reduced to normal level in the murine liver tissue.
2.4 the relative amount of MDA in the mice serum
The relative amount of MDA is as shown in table 4 in the mice serum.Behind the mice lead poisoning, the O.D value of MDA is significantly higher than blank group (P＜0.01) in its serum, the O.D value of MDA reduces than independent lead poisoning group in the Pb+50 group mice serum, but do not reach significant level, the O.D value of MDA descends significantly (P＜0.05) in the Pb+150 group mice serum, the O.D value decline of MDA extremely remarkable (P＜0.01) in the Pb+300 group mice serum.Illustrate that the bracken flavone of feeding can remove oxygen-derived free radicals in the mice body, reduce mice body endogenous cause of ill lead poisoning and a large amount of MDA content that produces, reduce level of lipid peroxidation.Relatively the Pb+50 group is organized with Pb+150, show that latter MDA content descends to some extent, but the two difference does not reach significantly extremely level (P〉0.05), and the Pb+300 group is compared with the Pb+150 group, the MDA content of Pb+300 group further descends, the two difference reaches utmost point significant level (P＜0.01), illustrates that the bracken flavone amount of feeding is big more, reduces the ability of organizing level of lipid peroxidation that causes because of lead poisoning and strengthens.
Table 4. is fed bracken flavone and lead poisoning to the influence of MDA content in the mice serum (X ± s)
Compare * P＜0.05 * * P＜0.01 with the Pb processed group
3 conclusions
Give the saturnine mice bracken flavone of feeding, mice MDA content reduces, from murine liver tissue and serum the MDA changes of contents as can be seen, with the increase of the amount of the bracken flavone of feeding, MDA content decline scope is just big more.Prompting, the decline of MDA content is positive correlation to a certain degree in the scale of feeding of bracken flavone and mouse liver and the serum.
MDA content is along with the increase of bracken flavone scale of feeding is on a declining curve in the mice body, MDA content in MDA content and the hepatic tissue in the comparison serum, as can be seen relatively during low dosage bracken flavone (50mg/kg.bw), obvious variation does not take place in MDA content in the serum, reduces and occurred significant level of lipid peroxidation in the hepatic tissue.When 150mg/kg.bw bracken flavone scale of feeding, the decline of MDA content reaches significant level (P＜0.05) in the serum, and MDA content fall reaches utmost point significant level (P＜0.01) in the hepatic tissue.Prompting, the oxidation of hepatic tissue and antioxidant system may be subjected to plumbous influence easilier, and the MDA changes of contents of hepatic tissue more can reflect the lipid peroxidation situation of body.
Give the saturnine mice bracken flavone of feeding, find that the SOD vigor gos up, and along with the increase of bracken flavone scale of feeding, the SOD vigor continues to rise, when the bracken flavone scale of feeding reached 300mg/kg.bw, the SOD activation recovering was normal.Illustrate that the bracken flavone of feeding can repair the infringement of the cellular oxidation that lead poisoning causes, and the ability of the synthetic SOD of body is recovered.The plumbous mechanism of SOD activity influence being still needed with bracken flavone further deeply inquired into, but the bracken flavone of feeding is sure to cellular oxidation injury repairing effect under the lead poisoning.
Therefore think that extract of bracken flavone is having remarkable effect aspect alleviation or the treatment lead poisoning.
Claims (3)
1. the application of extract of bracken flavone in preparation Plumbum removing and alleviation lead poisoning medicine, it is characterized in that: extract of bracken flavone is in preparation Plumbum removing and the application of alleviating in the lead poisoning medicine.
2. the application of extract of bracken flavone according to claim 1 in preparation Plumbum removing and alleviation lead poisoning medicine, it is characterized in that: the application of extract of bracken flavone in preparation Plumbum removing and alleviation lead poisoning medicine, described extract of bracken flavone is auburn slurry, and the mass fraction that flavone accounts for slurry is 10-15.
3. the application of extract of bracken flavone according to claim 1 in preparation Plumbum removing and alleviation lead poisoning medicine, it is characterized in that: the application of extract of bracken flavone in preparation Plumbum removing and alleviation lead poisoning medicine, described extract of bracken flavone is auburn Powdered thing, and the mass fraction that flavone accounts for the crude flavonoid powder powder is 15-30.
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| CN102389516A (en) * | 2011-11-21 | 2012-03-28 | 上海市第二中学 | Pharmaceutical composition for treating lead poisoning, preparation method and application thereof |
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| CN103860608A (en) * | 2014-03-12 | 2014-06-18 | 陈乃富 | Application of fiddlehead flavonoids in preparing medicament for resisting hyperuricemia diseases |
| CN109030474A (en) * | 2018-06-13 | 2018-12-18 | 迦娜生物科技（武汉）有限公司 | A kind of lipid free radical urine detection reagent and its application |
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| 陈乃富.蕨菜黄酮对小鼠体内丙二醛含量的影响.《生物学杂志》.2006,第23卷(第4期),30-33. * |
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