CN100575476C - The bacterial strain B 211 of control cotton verticillium wilt - Google Patents
The bacterial strain B 211 of control cotton verticillium wilt Download PDFInfo
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- CN100575476C CN100575476C CN200810123738A CN200810123738A CN100575476C CN 100575476 C CN100575476 C CN 100575476C CN 200810123738 A CN200810123738 A CN 200810123738A CN 200810123738 A CN200810123738 A CN 200810123738A CN 100575476 C CN100575476 C CN 100575476C
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Abstract
The present invention relates to prevent and treat the biocontrol strain B221 of cotton verticillium wilt, belong to the farm crop technical field of preventing and curing diseases.Through being accredited as ball Chaetomium (Chaetomium globosum).Culture presevation number is CGMCC NO.2493.Bacterial classification is at 25 ℃ of 200rpm shaking culture of PDA nutrient solution 7d, with obtaining its spore suspension, viable bacteria total concn 10 in the finished product after 8 layers of sterile gauzes filtration
9Individual spore/milliliter obtains biocontrol fungicide.Bacterial strain is 4 ℃ of preservations in the PDA inclined-plane, and the B221 strains expressed goes out dull and stereotyped preferably antagonistic effect, greenhouse preventive effect and the short fruit of coming into force.Test shows that bacterial strain B 211 greenhouse preventive effect reaches 58.96%, and the increase of biomass reaches 51.20%; There is bigger potential development to become business-like viable bacteria biological prevention and control agent.
Description
One, technical field
The present invention relates to prevent and treat the single bacterial strain of cotton verticillium wilt, belong to the farm crop technical field of preventing and curing diseases, be exclusively used in the non-environmental pollution control of cotton verticillium wilt.
Two, background technology
Big beautiful to take turns the cotton verticillium wilt that a spore (Verticillium dahliae Kleb.) causes be worldwide, destructive disease by verticillium dahliae, have the title of cotton " cancer ".From 1914 first since U.S. report, each big cotton region now all over the world.China imports into when nineteen thirty-five is introduced this word cotton by the U.S., and the back constantly enlarges with the cotton seed allocation and transportation.In recent years, cotton verticillium wilt is each cotton region Continuous Flow behavior evil at home, and especially 2002～2003 years, each cotton region big area was popular in the whole nation, and onset area is up to 3 * 10
6Hm
2More than, Cotton Production is constituted greatly threat.
Because this disease is the vascular bundle diseases of propagating by soil and straw, so difficulty of prevention and cure is bigger.There is not the effective chemical agricultural chemicals in the chemical control to cotton verticillium wilt at first, at present.Even have, use chemical pesticide not only can produce pesticide residue, and also can occur the resistance problem of verticillium dahliae along with the use year by year of chemical pesticide.Secondly, China mainly is that to adopt seed selection to select disease-resistant variety for use be main comprehensive control measures to this disease at present.But at present the disease resistance of Cotton in China kind can only reach anti-to disease-resistant level, causes the still continuous popular harm under the suitable situation of envrionment conditions of this disease.
In addition, along with expanding economy, the raising of people's living standard, people more and more pay attention to food-safety problem, and green crop, organic farm products etc. are favored by the people also more and more.Society has promoted to utilize nuisanceless method to control the research of harmful organism to the demand of safety, heath food.Logical research practice in a few years, some biological bactericides have now been developed, as agricultural streptomycin, jinggangmeisu, the former can be made into the 200mg/L liquid irrigating root and prevent and treat vegetable bacterial soft rot, bacterial wilt, and the latter is made into 1000-1500 times of liquid control vegetables anthrax, oidium.
Because still lack high resisting high-yield kind of ideal and beneficial agents up to now, and the problem of chemical prevention environmental pollution becomes increasingly conspicuous, so in today of the nuisanceless production technology of emphasis on development, biological control will further be paid attention to and develop as a kind of new approaches of control cotton verticillium wilt.
Three, summary of the invention
Technical problem does not have the biocontrol strain preparation present situation of higher preventive effect at cotton verticillium wilt, provides exploitation a kind of single bacterial strain and microbial inoculum thereof of preventing and treating cotton verticillium wilt, is exclusively used in the control cotton verticillium wilt, the preventive effect height of bacterial strain, and the volume increase function is arranged.
Technical scheme
The bacterial strain B 211 of control cotton verticillium wilt was ball Chaetomium (Chaetomium globosum), was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 05 09th, 2008, and culture presevation number is CGMCC NO.2493.
The method for preparing biocontrol fungicide with the bacterial strain B 211 of preventing and treating cotton verticillium wilt is:
The bacterial strain B 211 of above-mentioned control cotton verticillium wilt 25 ℃ of 200rpm shaking culture of PDA nutrient solution 7 days, is obtained its spore suspension microbial inoculum finished product after filtering with 8 layers of sterile gauze, viable bacteria total concn in the microbial inoculum finished product is adjusted to 10 with counting method of blood cell
9Individual spore/milliliter.Its application method is that the spraying before crop is transplanted of bacterial strain preparation is used, and also can make the compost use, or the root use is irritated in the dilution back when transplanting.
Beneficial effect
The present invention compares with existing cotton verticillium wilt Prevention Technique, its advantage and positively effect show: the present invention is specially at the biocontrol strain of cotton verticillium wilt exploitation, thereby significantly improves comparing prevention effect with other broad spectrum biological disinfection agent at present aspect the control cotton verticillium wilt.Owing to be biological bacterial strain preparation, there are not a series of problems that use brought fully because of chemical pesticide, thereby help the nuisanceless production of crop, the peasant can or reduce the consumption of other control cotton verticillium wilt chemical pesticides, this not only can be the peasant and reduces expenses, and helps the outlet of crop.Simultaneously, the biocontrol strain preparation increases production function in addition, can be the peasant and increases income.
B221 bacterial strain preparation can be sprayed in the fertilizer before transplant in the greenhouse, buries in the soil in whole ground process with fertilizer then, also can irritate root and use when planting seedling.After planting seedling, bacterial strain works by multiple Biocontrol Mechanism, thereby reaches the effect of control cotton verticillium wilt.Test shows, uses B221 bacterial strain preparation that cotton verticillium wilt is had to a certain degree prevention effect in the greenhouse, reaches 58.96%; B221 bacterial strain preparation also has to a certain degree effect of increasing production to cotton, reaches 51.20%.
Four description of drawings
In Fig. 1 greenhouse test the B221 microbial inoculum sowing after 55 days to the prevention effect of cotton verticillium wilt
Annotate: a left side-CK, the right side-B221; Preventive effect
The B221 microbial inoculum comes into force really to the short of cotton verticillium wilt after 55 days in sowing in Fig. 2 greenhouse test
Annotate: a left side-CK, the right side-B221; Short giving birth to
In Fig. 3 greenhouse test the B221 microbial inoculum sowing after 55 days to cotton verticillium wilt the short fruit (root) of coming into force
Annotate: a left side-CK, the right side-B221; Short give birth to (root)
Five embodiments
The evaluation of 1 bacterial strain
The big beautiful biocontrol strain B221 that takes turns a spore (Verticillium dahliae Kleb.) of control verticillium dahliae, it is to be taken place in the serious field piece by Rudong County, Nantong City pick T-shaped bank village, port town cotton verticillium wilt, the root portion of cotton healthy tree is from getting, the contriver is to the dull and stereotyped antagonistic activity of bacterial strain B 211, cellulase-producing, proteolytic enzyme, chitinase is active and utilize the mensuration of phosphorus and nitrogen element to study, find that bacterial strain B 211 has stronger dull and stereotyped antagonistic activity to verticillium dahliae (Verticilliumdahliae), but do not have proteolytic enzyme, cellulase and chitinase activity, can not utilize phosphorus and nitrogen element, bacterial strain PDA culture medium culturing, 4 ℃ of preservations in the PDA inclined-plane.The contriver has also carried out greenhouse preventive effect and the short experimental study of giving birth to it, finds that B221 bacterial strain preparation has good preventing disease production-increasing effect, and preventive effect reaches 58.96%, and the increase of biomass reaches 51.20%, has bigger potential development to become business-like viable bacteria biological prevention and control agent.
Fungi B221 to be measured is connected to the PDA substratum, cultivated 5 days for 25 ℃, scrape with the aseptic operation cutter and to get sophisticated mycelia, according to Wang et al. method (Wang, H., Qi, M., and Cutler, A.J.1993 A simple method ofpreparing plant samples for PCR.Nucleic Acids Res.21,4153-4154.) DNA of extraction fungi.Utilize primer I TS
1And ITS
4(ITS
1: 5 '-TCCGTAGGTGAACCTGCGG-3 ', ITS
4: 5 '-TCCTCCGCTTATTGATATGC-3 ') (Sheng Xing Bioisystech Co., Ltd is synthetic) carries out the ITS zone of pcr amplification fungi to be measured.Institute responds and adopts 25-μ L system: ITS
10.25 μ L, ITS
40.25 μ L, 10 * PCRbuffer, 2.5 μ L, 25mM MgCl
21.5 μ L, 2.5mM dNTP 1 μ L, 5 μ/ml r-Tag polymerase (Takara) 0.25 μ L, ddH
2O 18.25 μ L, DNA 1 μ L is a template.The PCR reaction conditions is: 94 ℃ of 5min, 94 ℃ of 30s, 55 ℃ of 30s, 72 ℃ of 30s, 35 cycles, 72 ℃ of used for of with 10min extension at the final cycle (Wang et al., 1993).The PCR product detects with the agarose gel electrophoresis of 1% (w/v), and voltage 90V, 15min, the EB 30min that dyes finds target stripe (500-600bp), adopts gel to reclaim test kit (H.Q.﹠amp; .Q.Gel Extraction Kit II) required band is reclaimed.Target fragment is connected to carrier pMD18-T, changes over to then in the E.coli Top-10 competent cell, obtain positive colony.Obtain ITS zone nucleotide sequence by order-checking, use Basic Local Alignment Search Tool (BLAST) to compare in NCBI (NationalCenter for Biotechnology Information) Nucleotide Sequence Database in the Internet, obtaining qualification result B221 is ball Chaetomium (Chaetomium globosum).
2 greenhouse efficiency tests
With pathogenic bacteria (Verticillium dahliae Kleb.) (Li, X.L., Li, Y.and Zhang, T.Y.2003 Studies onCotton Verticillium Wilt Control by Fungal Antagonists.Acta Gossypii Sinica 15,26～28.) be connected to the PDA substratum, 25 ℃ of thermostat containers were cultivated after 5 days, and the picking mycelia is in PDA nutrient solution (Li, X.L., Li, Y.andZhang, T.Y.2003 Studies on Cotton Verticillium Wilt Control by Fungal Antagonists.Acta Gossypii Sinica 15,26～28.) in, 200rpm, cultivated 7 days for 25 ℃, after 8 layers of sterile gauze filter, utilize the blood cell number scale to record spore suspension concentration, be connected in the sterile soil, make every gram soil contain 3.6 * 10
7Individual spore, pathogenic bacteria are grown surely and according to the method described above biocontrol microorganisms B221 are connected to after 10 days that (concentration is every gram soil about 10 in the soil
7Individual spore), sows kind simultaneously and grind 15 cotton coating seeds, establish the contrast that is treated to of only inoculating pathogenic bacteria for the Shandong cotton.Each is handled and repeats 3 times, and each repeats 20 strain cottons.Sow statistics after 55 days, calculate disease index and preventive effect.
The grade scale (greenhouse seedling stage) of morbidity intensity during investigation result: 0 grade-strong seedling, cotyledon, true leaf do not have symptom; I level-1～2 a cotyledon morbidity, true leaf does not show symptom; II level-cotyledon and 1 true leaf morbidity; III level-2 slice or 2 above true leaves morbidities; IV level-blade come off in a large number or cotton withered.Disease index and preventive effect calculation formula are as follows:
Seedling stage, results from pot experiment test showed that (table 1, Fig. 1), the cotton of inoculation B221 shows prevention effect to a certain degree.
Table 1 biocontrol microorganisms is used the back 55 days potted plant preventive effects to cotton verticillium wilt
Short the giving birth in 3 greenhouses tested
Give birth in the test short, the treatment group plant only inoculates the spore suspension of antagonism bacterium B221, establishes clear water processing plant and is contrast.Method is with the greenhouse efficiency test.Sow the plant height of measuring cotton after 55 days, root is long, and fresh weight, and the increase of calculating biomass are in order to estimate the influence of biocontrol microorganisms to the plant growth-promoting effect.The increase calculation formula of biomass is as follows:
Table 2 biocontrol microorganisms is used the back 55 days potted plant short fruits of coming into force to cotton verticillium wilt
Claims (3)
1, the bacterial strain B 211 of control cotton verticillium wilt, it is characterized in that, this bacterial strain was ball Chaetomium (Chaetomiumglobosum), was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 05 09th, 2008, and culture presevation number is CGMCC NO.2493.
2, the application of the bacterial strain B 211 of the described control cotton verticillium wilt of claim 1 aspect control greenhouse cotton verticillium wilt.
3, use the biocontrol fungicide of the bacterial strain B 211 preparation of the described control cotton verticillium wilt of claim 1.
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| CN100575476C true CN100575476C (en) | 2009-12-30 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX368885B (en) * | 2013-11-06 | 2019-10-21 | Texas A & M Univ Sys | Fungal endophytes for improved crop yields and protection from pests. |
| CN105368720B (en) * | 2015-11-04 | 2019-03-26 | 中国农业科学院棉花研究所 | Cotton endogenetic fungus CEF-082 and its application in cotton verticillium wilt prevention and treatment |
| CN106591263B (en) * | 2016-12-16 | 2019-12-03 | 中国农业科学院棉花研究所 | The endogenous chitinase of verticillium dahliae and its encoding gene and application |
| CN108377893A (en) * | 2018-02-13 | 2018-08-10 | 东北农业大学 | A kind of rapid detection method of eggplant verticillium wilt disease resistance |
| CN111944789B (en) * | 2020-09-02 | 2021-11-30 | 鲁东大学 | Method for producing chitinase by fermenting chaetomium globosum and application thereof |
| CN114907992B (en) * | 2022-05-18 | 2023-06-16 | 塔里木大学 | Strain C11 antagonizing plant pathogenic bacteria and application thereof |
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Non-Patent Citations (6)
| Title |
|---|
| 油菜内生球毛壳菌抑菌作用初步测定. 陈利军等.河南农业科学，第7期. 2005 |
| 油菜内生球毛壳菌抑菌作用初步测定. 陈利军等.河南农业科学，第7期. 2005 * |
| 球毛壳菌几丁质酶3基因的序列分析. 金红星等.东北林业大学学报，第32卷第4期. 2004 |
| 球毛壳菌几丁质酶3基因的序列分析. 金红星等.东北林业大学学报，第32卷第4期. 2004 * |
| 用于棉花病害防治的生物资源. 李玉奎.中国农学通报，第7卷第3期. 1991 |
| 用于棉花病害防治的生物资源. 李玉奎.中国农学通报，第7卷第3期. 1991 * |
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Address after: 111 Baizhu Road, Baima Town, Lishui District, Nanjing City, Jiangsu Province 211225 Patentee after: Nanjing Nongda Bio-source Pesticide Creation Co.,Ltd. Address before: 210095 chemical building, No.6 Tongwei Road, Nanjing City, Jiangsu Province Patentee before: Nanjing Nongda Bio-source Pesticide Creation Co.,Ltd. |
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| CP02 | Change in the address of a patent holder | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091230 |
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| CF01 | Termination of patent right due to non-payment of annual fee |