CA1295784C - Peptide immunostimulants - Google Patents
Peptide immunostimulantsInfo
- Publication number
- CA1295784C CA1295784C CA000523519A CA523519A CA1295784C CA 1295784 C CA1295784 C CA 1295784C CA 000523519 A CA000523519 A CA 000523519A CA 523519 A CA523519 A CA 523519A CA 1295784 C CA1295784 C CA 1295784C
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- Prior art keywords
- compound
- methyl
- salt
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- carbon atoms
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/10—Tetrapeptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
- C07K5/0215—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link containing natural amino acids, forming a peptide bond via their side chain functional group, e.g. epsilon-Lys, gamma-Glu
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/06—Dipeptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PEPTIDE IMMUNOSTIMULANTS
Abstract Peptide compounds of formula 1, pharmaceutically acceptable base saits thereof, pharmaceutical compositions and their use as antiinfective agents where R1 is alkyl, cycloalkyl or cycloalkylmethyl; R2 is hydrogen or alkyl and R3 is hydroxy or an amino acid residue of the formula
Abstract Peptide compounds of formula 1, pharmaceutically acceptable base saits thereof, pharmaceutical compositions and their use as antiinfective agents where R1 is alkyl, cycloalkyl or cycloalkylmethyl; R2 is hydrogen or alkyl and R3 is hydroxy or an amino acid residue of the formula
Description
5~
- PEPTID~ I~MUNOSTIMUIANT~
. ., ~ his invention relat~s to novel acyl glutamic acid containing peptides useful as immunost~lant and antiinfective agents; to phar~aceutical compo~itions thereof and to the use thereof in treating inections-~
~ he relatively new field o~ immu~opha~macology, andparticularly that segment thereo~ which qeal8 with immunomodulation, continues to develop at a rapid pace.
A variety of ~aturally occurring co~po~nd~ has been investigated, including the tetrapeptide tuftsin, known chemically as N2-tl-(N~-L-thr~onyl~L-ly~yl)-L-prolyll-L-arginine. ~uch attention has been directed to 1~ synthetic peptidoglycan derivati~es, ~specially those known as muramyl dipeptides. For summaries of the wide range of com~ounds investigated as Lm~uno~odulators, and especially as immunostimula~ts, attention is dir~cted to Duker et al., Annu. Rep. ~ed. ChemO, 1~ 6~167 (1979), Lederer, J~ ~ed. Chem., 23, 819-825 ~19~0) and tc J. ~salovec, ~ of the ~uture, 8, 615-638 ~1983).
Immunostimulant peptides have been described in a number of patent specifications:
L Alanyl-alpha-glutaric acid N acyl dipeptides in German 3,024,355, published January 15, 1981, tetra- and penta-peptides containing D~alanyl-L-glutamyl moieties or L-ala~yl-D-gluta~yl moieties in British 2,053,231, published Fe~ruary ~, 1981 and Germa~
3,024,281, published January 8, 1981~ respectively; and acyl-alanyl-gamma-D-glutamyl tripeptide derivatives in which the C-terminal a~ino acid is lysine or diaminopimelic acid in German 3,024,369, published January 150 1981; a~d -2- 72222-~
- lactoyl tetrapeptides composed of N-lactylalanyl, glutamyl, diaminopimelyl an~ carboxymethylamino components in EP-11283, publi,shed ~ay 23, 1980.
Furthex immunosti~ulant ~polypeptides having the 5 formula ~A) -(~NCHCO)n-EN-C~ R
R ~ 1~2)m co~ CH_R4 ~A) (1~2)3 R6~ R5 wherein R1 is hydrogen or acyl; R2 is nter alia hydrogen, lower alkyl, hydroxymethyl, benzyl; R3 and R4 are each hydrogen~ carboxy, -CoNR7R8 wherein R7 is hydrogen, lower alkyl optionally substituted with hydroxy; and R8 is mono- ~icarboxy lower alkyl; R5 is ~yarogen or carboxy with the proviso tha~ when o~e of R4 and RS is hydrogen, the other is carboxy or CoNR7R8;
is hydrogen; m is 1 to 3 a~d ~ is 0 to 2, and derivatives thereof in ~hich the carboxy and amino groups are protected are di~closed in U.S. Patents 4,311,640 and 4,322,341; EP applications 25,4~2; 50,856;
51,812; 53,388; 55,~46 and 57,419.
i7~3~
- PEPTID~ I~MUNOSTIMUIANT~
. ., ~ his invention relat~s to novel acyl glutamic acid containing peptides useful as immunost~lant and antiinfective agents; to phar~aceutical compo~itions thereof and to the use thereof in treating inections-~
~ he relatively new field o~ immu~opha~macology, andparticularly that segment thereo~ which qeal8 with immunomodulation, continues to develop at a rapid pace.
A variety of ~aturally occurring co~po~nd~ has been investigated, including the tetrapeptide tuftsin, known chemically as N2-tl-(N~-L-thr~onyl~L-ly~yl)-L-prolyll-L-arginine. ~uch attention has been directed to 1~ synthetic peptidoglycan derivati~es, ~specially those known as muramyl dipeptides. For summaries of the wide range of com~ounds investigated as Lm~uno~odulators, and especially as immunostimula~ts, attention is dir~cted to Duker et al., Annu. Rep. ~ed. ChemO, 1~ 6~167 (1979), Lederer, J~ ~ed. Chem., 23, 819-825 ~19~0) and tc J. ~salovec, ~ of the ~uture, 8, 615-638 ~1983).
Immunostimulant peptides have been described in a number of patent specifications:
L Alanyl-alpha-glutaric acid N acyl dipeptides in German 3,024,355, published January 15, 1981, tetra- and penta-peptides containing D~alanyl-L-glutamyl moieties or L-ala~yl-D-gluta~yl moieties in British 2,053,231, published Fe~ruary ~, 1981 and Germa~
3,024,281, published January 8, 1981~ respectively; and acyl-alanyl-gamma-D-glutamyl tripeptide derivatives in which the C-terminal a~ino acid is lysine or diaminopimelic acid in German 3,024,369, published January 150 1981; a~d -2- 72222-~
- lactoyl tetrapeptides composed of N-lactylalanyl, glutamyl, diaminopimelyl an~ carboxymethylamino components in EP-11283, publi,shed ~ay 23, 1980.
Furthex immunosti~ulant ~polypeptides having the 5 formula ~A) -(~NCHCO)n-EN-C~ R
R ~ 1~2)m co~ CH_R4 ~A) (1~2)3 R6~ R5 wherein R1 is hydrogen or acyl; R2 is nter alia hydrogen, lower alkyl, hydroxymethyl, benzyl; R3 and R4 are each hydrogen~ carboxy, -CoNR7R8 wherein R7 is hydrogen, lower alkyl optionally substituted with hydroxy; and R8 is mono- ~icarboxy lower alkyl; R5 is ~yarogen or carboxy with the proviso tha~ when o~e of R4 and RS is hydrogen, the other is carboxy or CoNR7R8;
is hydrogen; m is 1 to 3 a~d ~ is 0 to 2, and derivatives thereof in ~hich the carboxy and amino groups are protected are di~closed in U.S. Patents 4,311,640 and 4,322,341; EP applications 25,4~2; 50,856;
51,812; 53,388; 55,~46 and 57,419.
i7~3~
gitaura et al., J. ~ed. Chem., 25, 335-337 (1982) report N2-~gamma-D-glutamyl~-m~so-ZtL),2~D)-di~mino-pimelic acid ~ the ~inimal ~tructure cap~ble o~
ellciting.a biological r~sponne characteri~tic of the compound of formula ~A) wherein n i5 1; Rl ~ 5 CH3C~O~)-CO-; R2 ~s CF13: each of R3 and R5 ~8 -COO~, R~
i8 -CONEICl~2COO~l and R6 i3 ~. 5aid compound oP fonnula (A) i~ Icnown as FR-156.
l ~he novel i~munost~mulant~ of the pr~ent invention are of the formula O
Rl ~ H \ D / CO~R4 I n ~CR2)2CON~IHC R3 ' and a pharmaceutically acceptable base salt thereof, wherein R1 is cycloalkyl of four to seven carbon atoms, alkyl of two to ten carbon atoms or cycloalkylmethyl of six to eight carbon atoms; R2 is hydrogen; and R3 is an amino acid residue of the formula D
where X is hydrogen, alkyl of one to two carbon atoms or hydroxymethyl and n i8 an integes o O to 4; and R~ and RS ar~ each hydrogen, alkyl of one to ~ix carbon atoms, cycloalkylmethyl of ~ix to eight carbon atoms or benzyl.
'' ,~", - ,-.: .: ::..:
. .,, ., :;
. ., ,~ , . -: .:
~ 7~
; , -4- :
:
A preferred group of compounds are those where Rl ~
is alkyl of five to eight carbon atoms, R2 is hydrogen, R3 is said amino a~id residue where X, n and R5 are as defi~ed, and R4 is hydrogen. Particularly pref~rr~d are S those compounds within the group whexe n i3 0 and R5 is hydrogen, said alkyl or ~yclohexylmethyl. Especially pre~erred compounds are those wherein Rl i~ (R~S) 2-ethyl-1-butyl, R~ is hydrcgen and ~ i~ methyl, Rl i8 ~R,S~ 3-heptyl, R5 is hydrogen and X is methyl, Rl is ~R,S) 2-methyl-1-pentyl, R5 is hyarogen and X ~g methyl, Rl is (R,S) 2-heptyl, R5 is hydrogen and X is methyl, R~
is ~R,S) 2-ethyl-1-pentyl, R5 is hydrogen and X is methyl, Rl is (R,S) l-hexyl, R5 is hydrogen and X is IS methyl, Rl is ~R,S) 2-ethyl-1-he~yl, R5 i8 hydrogen and X is methyl, Rl is (S) or ~R,S) 2-methyl-1-hexyl, R5 is hydrogen and X is methyl, Rl is IS) or (~,S) 2-ethyl-l-hexyl, X is methyl and R5 is hydrogen and Rl is l-hexyl, X is methyl and R5 is ~ydrogen. Also espe-cially preferred is the compound wherein Rl is l-hPxyl, X is hydrogen and n i~ 3. Especially pr~ferred esters are those wherein Rl is (R,S) 2-ethyl-1-pe~yl, X is methyl and R5 is n-butyl, i_butyl or cycloh~xylmethyl, R1 is (S) or (R~S) 2-methyl-1-hexyl, X i5 methyl and R5 is n-butyl, i-butyl or cyclohexy}methyl and Rl is ~S3 or (R~S3 2-ethyl-1-he~yl, X is ~ethyl and R5 is n-butyl, i-butyl or cyclohe~ylmethyl.
R ~econd preferred group of compounds are tho~e wherein R1 is cycloalkyl of four to ~even carbon atoms, R2 is hydrogen and R3 is said amino acid residue where n ~-: is 0, X is alkyl o~ one to two c~r~on atoms and R~ and R5 are each hydrogen. E~pe~ially preferred within ~his group is the compo~nd ~here R~ is ~yclohexyl and X is methyl.
.
~2~3~7t~4 A thixd preferred ~roup of compound$-are those wherein Rl is alkyl of five to eight car~on atoms, R2 is hydrogen, R3 is ~ai~ ~mino ~cid residue where X is hydrogen or alkyl o~ one ~o two carbon atoms, n i8 an integer of O to 4 and R5 is hydrogen and R4 is ~lkyl o~
one to 5iX carbon atoms, c~cloalkylmethyl of six to eight carbon atoms or benzyl.
The present invention is al~o directed to ~ pharma~
ceutical composition in unit do~age form comprising a phar~aceutically acceptable carrier and a~ antiin~ect~ve or i~muno~timulant effective amount of a compound of formula 1 and a use for trea-ting an inEection in a human ~uffering therefrom of a comound of formula 1.
1~
~ y pharmaceutically acceptable base salts o~ said compounds of formula 1 is meant salts ~ith inorganic or organic bases such as alkali metal and al~aline earth 2D metal hydroxides, ammonia, triethylamine, ethanolamine and ~i~yclohexylamine.
The configuration of the amino acid moieties which make up the compounds of formula 1 is ~ignificant as regards the pharmcological activity of said compounds.
The mo~t potent act~vity is observed in the compounds o~
for~ula 1 having the ~ter~ochemi~try indicated ~n ~aid for~ula. In those compounds of formula 1 where R2 a~d X
are other than hydrogen, the preferred stereochemi~try at said carbon is indicated as L and ~, respectively.
Also considered within the scope o~ the pre~ent invention are compounds of formula 1 where R3 is al~oxy, cycloalkoxy, aralkoxy or alkoxy substituted by one or more of the substituents ~elected from amino, dial~yl-ami~o, hydroxy, alkoxy and halo.
57~
- 5a - 72222-8 The present invention is further directed to a process or preparing a compound of formula (1) or a pharmaceutically acceptable base salt thereof. This process comprises:
[A] acylating the amino group in a peptide of the formula:
H2N ~
CH
I (L) o (CH2)2CONHCH~C-R3 (wherein R2, R3 and R4 are as defined above except that if they interfere the reaction, they may be blocked by a blocking group), with a carboxylic acid of the formula:
RlCOOH (4) (wherein Rl is as defined above) or an activated derivative thereof, followed by, if necessary, the removal of the blocking group, or [B] amidating the carboxyl group or the activated form thereof in a compound o the formula:
~; ~
7~
- Sb - 72222-8 RlCONH (D) / C2R4 fH ~L) (5) (CH2)2CONHCH-CO~OH
(wherein Rl, R2 and R4 are as de~fined above except that if they interere the reaction they may be blocked by a blocking group, and the carboxyl group attached to the group -CHR2- may be in an activated form), with an amino compound of the formula:
(D) 2 1 ( 2)nC~R5 (6) X
(wherein X, n and R5 are as defined above except that if they interfere the reaction, they may be blocked by a blocking group), followed by, if necessary, the removal of the blocking agent, thereby producing a compound, or [C] amidating a D-glutamic acid derivative of the ormula:
- 5c - 72222-8 l RlCNH (D) / C2R4 CH (7) I
( 2)2 (wherein Rl and R4 are as defined above except that if they interfere the reaction, they may be blocked by a blocking group), or an activated derivative thereof, with an amino compound of the formula:
(L) H2N-CHCOR3 (8) (wherein R2 and R3 are as defined above, except that if they interfere the reaction, they may be blocked by a blocking group), followed by, if nece~sary, the removal of the blocking group, and [D] if required, converting a compound (1) produced by any one of processes [A] through [C] into a pharmaceutically : acceptable base salt thereof.
, . . .
~,~f~
.
T~e compounds of for~ula 1 ~re prepared by a~y of several methods known to tho~e skilled in th~ art. ~he methodology involves the fo~tion of peptid~ linkages be~ween amino acid~ which, b~cause of their ami~o and carboxy groups, a~d ~requently the prese~ce o~ other reactive groups, necessitate the protection of said groups and/or the activation o~ such groups, particularly the carboxy group, in order ~o achieve a certain reaction or to optimize ~uch a reaction.
P~efef~b/~
n-ge~ two routes are employed in the synthesis of the compounds o~ fonmula 1. The first procedure ut lizes the coupling of the fragment RlCN~ ~ D / C02R~
7~ L :~
(C~2~2co~ aco2~
R~ :
with the a~ino acid fragment D
~2NCH (~ H2) nC02R5 2S The seco~d procedure co~rises acylation of the p~ptide ~2N \ D C02~4 C~ L O
- 30 1 ~
~2~2CON~ R3 ;' with ~he appropriate acid RlCO2~, In the exa~ples presented herein, cert~in protecting and activating groups are speci~iGally illustrated. ~owever, o~e skilled in the ~rt will S recognize that other protecti:ng or activ~ting groups could have been used. The choic~ of a particular protecting group is dependent to a gre~t extent UpOD the availability of the necessary reagent, i~s e~fect upon solubility of the ~protected~ compound, its ease of removal and the presence of other groups which might be effected by its use; i.e., its selectivi~y, ox its removal.
For example, it will be necessa~y, or at least desirable, in many reactions to protect the amino groups and/or the carboxy groups. The synthe~ic route chosen for the peptide synthesis may require removal of one or the other or both of said protecting groups in order to permit further reaction at t~e regenerated ami~o or carboxy group: i.e., the protecting groups used are reversible and, i~ ~ost instances, ar~ removable independently of each otherO Additio~ally, the choic~
of protecting group for a giv n amino group depends ~pon the xole of said amino group in the overall reaction schemen Amino protecting groups having varying levels of lability, iOe.; eas~ of removal~ will be used. The sam~ is true as regards car~oxy protecting yroups. Such groups are k~own in the art and atte~tion is dlrected to the re~iews by ~odansky et al., ~Peptide Synthesis~, 2nd : 30 Ed., John Wiley ~ Sons, ~.Y. (197~ reene, "Protective Groups in Organic Synthesis~ John ~iley ~ Sons, (l98l~; ~cCmie, WProtective ~roups in Organic Chemistry~, Plenum Press, N.Y. (1973~; and to Sheppard in ~Comprehensive Organic Chemistry, The Synthesis and j....
j:
- 8 ~ ~ 78~ 72222-8 R~actions of Organic Compounds", Pergaman Press, N.Y. (1979), edited by E. Haslam, Part 23.6, pages 321-339.
Conventional amino and carboxy protecting groups are known to those skilled in the art. Representative amino protect-ing groups, but by no means limiting thereof, are the following:
such as benzyloxycarbonyl, substituted or unsubstituted aralkyl such as benzyl, trityl, benzhydryl and 4-nitrobenzyl; benzylidene;
arylthio such as phenylthio, niotrophenylthio and trichlorophenyl-thio; phosphoryl derivatives such as dimethylphosphoryl and O,O-dibenzylphosphoryl; trialkylsilyl derivative~ such as trimethyl-silyl; and others as are described in U.S. Patent 4,322,341. The preferred amino protecting group is benzyloxycarbonyl. Procedures for substituting said group on a given amino group are well known, In general they comprise acyla-ting the appropriate amino compound with benzyloxycarbonyl chloride (benzylchloroformate) in a reaction-inert solvent, e.g., water, methylene chloride, tetra-hydrofuran, in the presence of a base (acid acceptor) e.g., sodium or potassium hydroxide when water is solvent; and, when an organic solvent is used, in the presence of a tertiary amine such as Cl_4 trialkylamines and pyridine. When an aqueous solvent system is used the pH of the reaction is held at about pH 8-10, and prefer-ably at pH 9. Alternatively, when the reactant; i.e., the com-pound, an amino group of which is to be protected, contains 'basic groups, it can serve as acid acceptor.
The acyl group, RlCO is introduced into the peptide by standard acylation procedures as by reacting said peptide with the ~r 9 - ~2~57~ 72222-8 appropriate acicl chloride or bromide in a reaction inert solvent.
Favored conditions are non-aqueous conditions including addition of a suitable base such as an organic base, preferably a tertiary amine such as triethylamine, N-methylmorpholine or pyridine. The preferred solvent is methylene chloride.
Representative carboxy protecting groups are various esters such as silyl esters, including trialkyl silyl esters, trihalosilyl es-ters and haloalkylsilyl esters; certain hydrocarbyl esters such as Cl_4 alkyl, espeeially t-butyl groups, benzyl and substituted benzyl esters, benzhydryl and trityl; phenacyl and phthalimidomethyl esters; certain substituted hydroearbyl esters sueh as chloromethyl, 2,2,2-trichloroethyl, cyanomethyl; tetra-hydropyranyl; methoxymethyl; methylthiomethyl; protected earbazoyl sueh as -CONH-NHR wherein R is an amino protecting group as disclosed above, espeeially benzyloxy earbonyl; and others as are described in U.S. Patent 4,322,341. A highly favored carboxy protecting group is the t-butoxycarbonyl group.
The protected amino and carboxy groups are converted to the unprotected amino and earboxy groups by proeedures known to those skilled in the`art. The benzyl group, the preferred pro-teeting groups for earboxy (as part of the proteeted carbazoyl group) groups are removed by eatalytie hydrogenation over palla-dium, especially palladium-on-earbon. Alternatively, said pro-teeting groups are removed by means o trifluoromethanesulfonie acid in trifluoroaeetie acid and in the presenee of anisole to suppress alkylation. The t-butoxyearbonyl group is readily remov-ed by treatment with dioxane saturated with hydrogen chloride.
-:~2~
-ln- ' Activation of carboxy groups as a menas of expe~ -diti~g a given reaction is methodology known to those skilled in the art~ Esp~cially use~ul in the ~erein described reaction ~equence are the ~5~ of anhydrid~s, S particularly cyclic anhydride~; and activated esters, such as those derived from N-hydroxyphthalimide and N-hydroxysuccinimide, ~oth of which are used in peptide syntheses.
The activated N-hydroxysuccinimide ester~ expedi~e subsequent reactions at said activated ester groups. As the skilled artisan will recognize other activating groups could be used. A group of particular i~terest i5 the N-hydroxyphthalimido group, which group is u~ed in the same manner as is the N-hydkoxysuccinimido group.
In both instances, a dehydrative ~oupling agent is used to form the activated ester. Representative of such coupling agents a~e l-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide ~bod-p-toluene sulfonate, dicyclohexyl car~odiimide, N,~'-carbonyldiLmida~ole, N-~3-dimethyl-aminopropyll-N'-ethylcar~odiimide hydrochloride, ethoxy-acetylene, diphenyl~etene and ~-ethyl-S-phenylisoxa-zolene-3'-sulfonate. The reaction conditions for using such coupling agents are well described in the litera 2s ture. In general they comprise the use of a react on-inert solvent and te~pera~ures ranging ~rom ~mbient to 100C. The above-mentioned carbodiimide reagents are favored since they penmit use of ambient reactiGn temperature and afford satisfac~ory yields of the 3n desired esters.
Upon completion of the coupling reactions leading to the final products, the variou~ protecting groups can be r~moved by t~e appropriate t~ch~iques previously discussed, and the compo~lnds of fonmula 1 isolated.
~ 5~1~34 :' ::, ~ he pharmaceutically acceptable base-salts of formula 1 compounds, where R3 is hydroxy or R4 or R5 is hydrogen, are obtained by treating a solution, preferably aqueous ~olution, thereof with ~ b ~e such a~
are enumerated above, generally in ~toichiom~tric proportions. The ~alt~ are isolated by evaporation or by precipitation.
The products of this invention are useful ~8 agents in mammals, including humans, for the cli~ical and therapeutic treatment of diseases caused by various patho~enic microorga~isms, especially gram-negative ~acteria. They are also use~ul as immu~o~tLnulants in mammals, including humans, having an inc~ease risk of infection due to existing or i~creasad risk of infection due to existinq or clinical~y-induced Lmmunosuppression.
The test procedure, which used C3H/HeN ~le mice from the Charles River Breeding Laboratory, is presented below. The mice wer~ acclimatized for 5 days before use and then treated either subcutaneou~ly ~SC) or orally ~P0) with various dilution~ ~100, 10, 1 and 0.1 mg/kg) of the test compound or place~o (pyrogen ~ree saline) usin~ a volume of 0.2 ml. The treatment re~iment was dependent on the infec~ious organism utilized: -24 and :
O hours before challenge for Rlebsiella ~ne~moniae in normal ~ice; and -3, -2 and -1 day before challenge for Escherichia coli or Sta~. aureus in immunocompromised mice. Challenge ~as administere~ intramuscul~rly ~IMl in the hip in the case of R. pneumoniae or intr~-peritoneally (IP) in t~e case of S. coli and ~Q~.
aureus. A volume of 0.2 ml. was u~ed for the challenge.
~ortality was recorded after 7 days in the case of R.
pneumoniae and after 3 days i~ ~he case of the other two microorganism challenges.
~lture Preparation: -K. pneumoniae, E. coli, or ~ . 2ureu~: theculture was ~treaked for purity fro~ fro~n blood st~ck on brain heart infusion (~I) agar. ~hree colonies w~re picked from the 18 hour plate culture and placed into 9 ml. of BHI broth~ The broth culture was grown for 2 hours at 37~Co on a rotary shaker after which 0.2 ml.
was streaked on the surface of several B%I agar slants.
Pollo~ing an 18 hour incubation at 37C., the slants lo were washed with B8I broth, the cultur~ density adjusted using a spectronic 20 and the appropriate dilution made to achie~ an LD90 challenge level in nor~al mice.
When used as antiinfective or immunos~imulan~
agents in humans, the compounds of thi~ invention are ,, conveniently admini~tered via the oral, subcutaneous, intramuscular, intravenous or intraperitoneal routes, generally in composition form. Such compositions include a pharmaceutical practice. For axample, they can be administered in the form of tablets, pills, powders or granules co~tai~ing such excipie~ts as starch, ~ilk su~ar, certain types of clay, etc. They can be administered in capsules, in admixtures with the same or equivalent excipients. They can also be administered in the form of oral suspensions, solutions, ~mulsions, syrups and elixirs whicb ~ay contain flavoring and colo~ing agents. For oral administration of the therapeutic agents of this invention, tablets or capsules containing from about 50 to about 500 mg. are suitable for most applica~ions.
The physician will determina the do~age which will be most suitable for an individual pa~ient and it will Yary ~ith the age, weight ~nd response of the particular patient and the ro~tP of a~ministratio~. The favore~
~2 ~ ~'7~ --13- ..
oral ~osage range, in single or divided do~es, is from about 1~0 to about 300 ~g/kg/clay. The favored parenteral dose is from a~out 1~0 to about 100 mg/kg/day: the preferred range from about 1.0 tc ~bout 2û n~g/kg/day. :
This invention also provided phar~aceutical compositions, including u~it dosage for~s, valuable for the use of the herein descri~ed co~pounds for the utilities disclosed herein~ The dosage form can be given in single or multiple doses, as previously not~d, to achieve the ~aily dosage effective or a particular utility.
The following examples are provided &olely ~or the purpose of further illustration. In the intere~t of brevity, the following abbreviations for pea~ shapes in the NMR spectra are used: s, ~inglet; d, doublet; t, triplet; q, quartet; m, multiplet. The terms mole and millimole are abbreviated as m and mm, respectively.
' '' "
;7~
' ;;:' . .
N-~eptanoyl-D-gæ~a-glutamyl-glycyl-D-alani~e (Rl S C~3(C~2)5; R~ DC~C~IcD
lA. N-heptansyl-D-gamma~glUt~myl(alpha benzyl ester)-~lyci~e ':
., To a solution o~ B97 mg. (13.0 ~m~ o~ glycine and 1.3 g. (13.0 mm) of triethyla~ine in 10 ~l. of water was added 5.0 g. ~11.2 mm1 of N-heptanoyl-Dogamma glutamyl 0 (alpha benzyl ester)-hydroxysuccinimi~e e~ter in lon ~1.
of dioxane, and the resulti~g reaction mixture allowed to stir at roo~ temperature for 80 ho~rsO ~h~ solution was poured into 300 ml. of ethyl acetate and the -~
separated organic pha~e washed wi~h 10~ hydrochlorac acid, water and a brine solution. The organic phase was separated, dried over magnesium sulfate and concentrated in vacuo to dryness. The residue ~a~ trituxated with _ ~ .
diethyl ether and filtered under x~itrogen, 3.43 g. q74~ -yield).
lB. N-heptanoYl-D~ qlutamy To a solution o~ 2 . O g. ~4 . 78 ~i of N -heptanoyl-D-gan~na-glutamyl (alpha benzyl ester)-~yci~e, 1.75 g. l5 ;
mm) of D-alanine benzyl ester ~-toluenesulfonic acid salt, 506 mg. I5 mm) of triethylamine and 675 mg. ~5 mmJ
of l--hydroxybenzotriazole in 100 ml. sf ~etrahydrofuran -~
was added 3.03 ~. (7.17 m~) of 1-cy~lohexyl-3~(2- :
morpholinoethyl)~arbodiimide metho-~-toluenesulfonate ::
and the reaction mixture stirred at room temperature ~or 18 hours. The reaction mixture was poured i~to 300 ml.
of ethyl acetate and the organic phase separated and was~ed wi~h 10% hydrochloric aeid, water, a saturated sodium bicarbonate 601ution and a brine ~olutio~. The organic phase was separated, dried ov~r magnesium , - :~L2 sulfate and concentrated in vacuo. The residue was triturated with diethyl ether and filtered under nitro-gen, 2.7 g. Two grams of the solid i~ 75 ml. of methanol with 403 mg. of lO~ palladium hydroxide on 5 charcoal was sha~en in a hydrogen atmo~phere at an initial pressure o 50 p~i for 4 hours. The catalyst was filtered and the filtrate evaporated u~der reduced pressure and the residue was clissolved in water and lyophilizad to give 1.23 g. (5~0% yield) o~ the deæired product as a white ~olid.
The NMR spectrum ~DMSO-d6) 3howed absorption at 4.35-4.2 (m, 2~)~ 3.83 (~, 2H), 2.35 (t, J~7Hz, 2B), 2.17 ~t, J-7~, 2H), 2.1-1.8 (m, 2~), 1.55-1.45 (m, 2H), 1.3 (d, J=6~z, 3~1, 1.17 (bs, 6H) and 0.75 (bs, 3~) ppm.
N-Hep~a~oyl-D-gamma-glutamyl-glycine (Rl = CR3~CY2)5;
R~ - ~; and R3 ~ O~) A solution containing 1~0 g. of ~-heptanoyl-D- :~
gamma-glutamyl (alpha benzyl ester)-glycine in 50 ml. of methanol was treated with 100 mg. of lO~ palladium hydroxide on charcoal and shaken i~ a hydro~en atmosphere at 50 psi for 3 hoursO The catalyst was filtered and the filtrate concentrated in vacuo, The residue was dissolved in hot water and ~vaporated i~
vacuo. The residue was redi~solved in water and lyophilized to give 630 mg. (83~ yield3 of the desired product as a white solid.
The NMR spectrum (D~SO-d6) showed absorption at 4.37-4.25 (~, 1~)~ 3.9 (s, 2~), 2.35 (t, J=7~z, ~
2.18 (t, J=6~z, 2~), 2.~-1.8 (m9 2~), 1.5-1.4 ~, 2H3, 1. 8 (~s, 6~) and O. 7 (bt, 3~) ppm. . .
EX~PLE 3 - .
N-~eptanoyl-D-ga~ma-glutamyl-qlycyl-glycin~
(~1 = CH3(ch23s; R2 ~ ~; and R3 2 2 3A. ~-heptanoyl-D-gamma-gluta~yl (alpha benzyl ester)-glYci~e hydroxysuccinamide ester To a cold 501UtiOII (0C.1 o~E 13rO g~ (31 mm) c~f N~heptanoyl-D-gamma-glutamyl (alpha ~enzyl est~r~ :
glycine and 3.91 g. (34 mm) o~ N-hydroxy~uc~in~mide in 400 ml. of ~e~rahydrofuran was added 7.0 g. (34 mm) oP
dicyclohexylcarbodii~ide, and the mi~ture ~llowed to stir at 0C. ~or on~ hour and at roo~ t~p~rature ~or 18 hours. The solids were filtered and the filtxate concentrated under reduced pre~sure. The residue was triturated with diethyl ether and filtered ~nd~r nitro-gen to give 15.4 g. (98~) of the desired intermediate.
3~. ~
To 2.0 g. (3.97 mm) of N-heptanoyl-D-gamma-glutamyl .
(alpha benzyl es~er)-glycine hydroxysuccinamide ester in ~o 100 ml. of dioxane was added ~46 ~g. 15.95 mm) of glycine and 0.55 ~1. l3~9 mm) of tr~ethylamine in 10 ~1. ', of water, and the resulting reactisn mixture allowed to stir at room temperatur~ for 13 hours. The 6~1ution was poured into 100 ml. of ethyl acetate and the organic ~.
layer washed with 2.5~ hydrochloric acid, water and a : brine solution. The organic layer was separa~ed, dried ~::
over magne~ium sulfate and concentrated to drynessO The '.
residue was triturated with diethyl ether and filtered under ni rogen to give 1.7 g. of white ~olid. One and 30 iive-t~nths grams of the solid in 75 ml.. of methanol containing ~00 mg. o~ lOS palladium hydroxide on carbon ! ~:
was shaken in a hydrogen atmosphere at 50 psi or 3 ~.
hours. The catalyst wa~ filtered and the filtrate '~
!
' .~.
' j`'.''''.
I':
i~
',:.
~57~3~
concentrated ~n vacuo. The residue w~s dissolved in water and lyophilize~ to give 1.12 g. ~90~ yie~d) of the desire~ product.
The NMR spectr~m ID~SO-d6) $howed ab~orption 8.2-8.0 (m, 3~), 4.19 ~m, 1~ .8-4.6 tm, ~), 2.25 ~t, J-7~z~ 2H), 2.1 (t, J-6Hz, 2~), 2.05-1.7 (~, 2~), 1.5 (m, 2~), 1.25 5bs, 6~) and o.as ~t, JY68Z~ 3H3 ppm.
EXAMPI~ 4 N-HeptanoyloD gamma-gluta~yl-glycyl-D~serine D
1 3(C~2)5; R2 ~ ~; and R3 ~ -N~CHtC~2O~)CO2~) ~ .
Starting with 2.0 g. (3.98 mm) of h-hep anoyl-D-g~mma-gl~tamyl (alpha benzyl ester)-glycine hydroxy~
succinamide e~ter, 780 mg. ~4.02 ~m) of O-benzyl-D-serine and .556 ml. (4.02 mm3 of triethylamine and following the procedure of Example 3B, 902 ~g. ~76%
yi~ld1 of the desired product i~ i~olated, m.p. 130- :~
132C.
The NMR spect~um (DMSO-d6) ~howed a~sorption at 8.36-7.94 ~, 3~t~ 4.~6-4.28 l~, lR)~ ~.28-4.08 (~, lH), 3.94-3.50 ~m, 4~I); 2.25 ~t, J~91~z, 2EI1. 2.17 It, J=g}~z, lH)~ 2.10-1.04 (~, 14~) and 0.9 (t, J=6~z, 3~ ppm.
EXA~IPL.E 5 ~-Heptanoyl-D-gamma-glutamyl-glycyl-D alpha-æminobutyric acid ~Rl ~ C~3(CH2)5 ; 2 R3- -N~C~(C~2C~33C02H
The procedure of ~xa~ple 3B was repeated, ~tar~ing with 2.0 g. (3.98 ~m) of N-heptanoyl-D-gamma-glutamyl (alpha benzyl ester)-glycyl hydroxysuccinamide ester, :~
400 mgO S4.02 mm) of D alpha-~minobutyric acia and .55S
ml. i4~02 m~ of triethylamine, to give 632 mg. (57%
yield) of the desired proauct, m.p. 140-141C.
. .
-lB-.
The NMR spectrum ~DMSO d6) showed absorption at 8.16-8.04 (m, 3~), 4.22-4.0B ~m, 2~, 3.8~-3.58 (m, 2~), 2.2 It, J~9~z, 2H~, 2.12 (t, J-g~z, 2~ .D4~1.0 ~m, 15~) and 0.35 ~, J~6~z, 6~1 ppm.
E ~ ]~ 6 N-~eptanoyl-D-gamma-glutamyl-glycyl-3-am~nopropionic 1 3~ 2~; R2 ~; and R3 - -N~(CH2)2C02H) Following the procedur~ of Example 3B and ~tarting with 1.5 g. (3.0 mm) of N-heptanoyl-D-gam~a-glutamyl ~alpha benzyl ester)-glycine hydroxysuccinia~ide ester, 350 mg. ~3.9 mm) of 3-aminopropionic acid and 055 ml.
(3.9 m~) of triethylamine, 500 mg. ~43~ yield) of the desired product was obtained, m.p. 135-138C.
The MMR spectrum ~DMS0-d6~ sh~wed absorption at 8.19-3.02 lm, 2~), 7.98-7.87 It, JG5Hz, 1~), 4.25-~.1 (m, 2~), 3.8-3049 (m, 2~), 3.44-3.1 (m, 2~), 2.4 (t, J36Hz, 2~), 2.22 (t, J=7~z, 2~), 2.14 ~t~ J-7~z, 2H), 2.1-1.67 tm, 2~), 1.6-1.17 (m, 8~) and 0.88 (t, J=6Hz, 3~ ppm.
~X~MPLE 7 ~-Heptanoyl-D-gamma-glutamyl-glycyl-~-aminobutyric ~1 C~35~H2~5 ; R2 s ~; and R3 ~ -N~CH~) C0 ~) .
The procedure of Example 6 wa~ r~peated ~ubsti-tuting 410 mg. (4.0 mm3 of 4-aminobutyric acid for the 3-aminopropionic acid to giYe 600 mg. l~o~ yield) of the desired produc~, m.p. 140-1~2C.
The N~R spect~um ~DMSOod6J ~howed ab~orption at 8.18-8.03 Im, 2~), 7.88 (bt, J=4~z, lB), 4.17-4.09 (m, 3~ 2B~, 3.81-3.48 (m, 2~, 2.32-2.0~ ~m, 6~)~ 2.0B-1908 Im, 12~) and 0.88 It~ J=6~z, 3~) ppm.
, --19~
~ ~ LE 8 N-~eptanoyl-D-gamma-glutamyi-gly~yl-5-amino-pentanoic acid (Rl ~CH (C~ ~ -; R2 ~ ~; and Sub5tituting 470 mg. (4.0 mm~ ~ 5-~inopentanoic acid ~or 3-aminobu~yric acid ,and ~oll~wing the proc~du~e of Example 6, 520 mg. ~423 yileld~ of the desir~d product was obtained, m.p. 122-124~C.
The NMR spectrum (DMSO-d6~ ~howed absorption at I0 8.25-7.9~ ~m, 2~), 7.85 It, J~5Hzt 1~), 4.25~.1 (m, 2~), 3.8~-3.46 (m, 2H), 3~24-2.9 (m, 2~), 2.21-2.08 (m, 6~), 2.08-1.2 (m, 14H1 and 0.88 ~t, J86~Z, 3~) ppm.
N-Heptanoyl-D-~amma-glutamylglycyl-6-aminohexanoic IS ~Rl ~3(C~2)5 ; ~2 H; and R3 ~ -N~(CH2~5C0 ~ .
The procedure of Example 6 wa~ again repea~ed, substituting s3n mg. ~4.0 mm) of 6~ ohexanoic acid for the 3-æminobutyric acid to give 520 mg~ S40~ yi~ld) of the desired produ~t as ~ whit~ foamO
The NMR spectrum (DMSO-d6) showed absorption at 8.28-7.9 (m, 2~), 7.82 (bt, J=4~z, 1~, 4.27-~.1 tm, 2~), 3.81-3.47 (mt 2~), 3.15-~.9~ ~m~ 2R), 2.3-2.08 (m~
6~), 2.08-1.18 (m, 16H) and 0.88 St, J~6~z, 3~) ppm.
N-Iso~aleryl-D-gamma-glutamyl-glycyl-D-alanine (~1 (C~332c~2-; ~2 ~ ~; and ~3 ~ CHIC~3lco~
lOA. ~
To a cold (0C.) solution of 100 ml. methylene chloride contai~ing 10 gO (57 ~m~ of ~-t-butyloxy-carbonylglycine, 20 g. ~57 mm) of D-alanine benzyl ester ~-toluenesulfonic acid salt and 5.77 g. ~57 ~) of ~5;7~3~
: ~ triethylamine was added 12.3 g. t60 ~) of dicyclohexyl-carbodiLmide and the resulting ~eaetion ~ixture allowed to warm to room temperature. After 18 hours the ~ixture was filtered and the filtrate ¢oncentrat~d in ~acuo.
The residue was disFolved in 200 ml. of e~hyl acetate and the organic layer washed with 2.5~ hydrochloric acid, water, a saturated sodium bicarbonate solution and a brine ~olution. The organic layer ~a~ ~eparated, ' dried over magnesium sul~ate and evaporated under r~duced pressure. To the resulti~g oil ZOO ~1. of dioxane saturated with hydrogen chloride was added.
A~ter 30 minutes 400 ml. of diethyl ether was added and the product filtered under nitrogen, 10.9 g. (70% .
lS yield).
lOB. N-t-butoxycarbonyl-D-gamma-glutamyl ~alpha benzyl ester)-kr~ ~n~m~ ester h yd ~ ~ lsu cc In C h~ t ~
~o 1500 ml~ of methylene chloride containing 50 g. .
(143 mmJ of N-t-butoxycarbonyl-D-gam~a glutamic acid alpha-benzyl e~ter and 17.3 g. ~150 ~ml of ~-hydroxy-succinamide was added 30.9 g. (15 mm) of aicyclohexyl-carbodii~ide and the resulting reaction ~ixtura allowed to stir at room temperature for 18 hours. The ~olids were filtered and the filtrate concentrat~d in vacuo.
The residue was triturated with diethyl ether and the :
~slids filtered l-nder nitrogen, 43.7 g. ~68% yield~
lOC. D-qamma-glutamyl (alpha benzyl esterl-glycyl-C~
:.' A solution containing 4~3 gO ~9.45 mmj of N-t-butoxycar~onyl-D-gamma-ylutamyl (~lpha benzyl ester) hydroxysuccinamide e~iter, 2471 g. (9.92 ~) of glycyl~
~-alanine ben~yl ester hydrochloride and 1.0 g. i9.92 mM) of triethyl~il~e in 100 ml. of ~ethylene chlor~de -~g~7~3~
was allowed to stir at room temperature for 18 hours, and was then concentrated in ~vacuo. Th~ residue was dissolved in 200 ml. of ethyl ~cetate and the ~olution washed with 2.5~ hydrochloric acid, ~ater, 10~ pota~siuM
carbonate and a brine ~olution. The organic pha~e was separated, dried over ~aqnesi~m sulfate and e~apor~ted under reduced pressure. The :residue was treated with 200 ml. of dioxane ~aturated with hydroge~ chloride and allowed to stir for 2 ho~rs. ~he soluti~n was concen- -trated to dryness in vacuo and the r~idue triturated ~ . ~
with diethyl e~her. The solids were ~iltered under nitrogen, 3.41 g. (73~ yield~.
lOD. -isovaleryl-D-gamma-gluta~y~ ycyl-D---alanine 15To a solution of l.0 g. (2~03 ~m) o~ D-gamma-glutamyl (alpha benzyl ester~-glycyl-D alanine benzyl ester hydrochloride and 616 mg. (6.09 mm) of triethyl-amine in 50 ml. of methylene chloride ~as added 490 mg.
14.06 mm~ of i~o-valeryl chloride ~nd the reaction 2D mixture stirred at room temperatur~ for 80 hours. ThQ
methylene chloride ~as evapoxated in vacuo and the residue dis~olved in ethyl acetate. The re~ulting solution was washed ~ith 2.5% hydrochloric acid, water 10~ potassium carbonate, water, and a brine solution. ..
The organic phase was separated, dried over magnesium sulfate and concentrated under vacuu~. ~he residue was triturated with die~hyl ether, iltered under nitrogen (910 mg.) a~d 700 ~g. dissolved in 50 ml. of mstha~ol.
Palladium hydroxide 200 mg. was added o the solution and the mixture shaken in a hydroge~ atmosphere at 50 psi ~or 3 hoursO The cataly~t was filtered and the sol~ent re~oved in vacuo. The re~id~e ~as dissolved in water and lyophilized to give 36~ ~g. t~5~ yield~ of the de~ired pr~duct~
ellciting.a biological r~sponne characteri~tic of the compound of formula ~A) wherein n i5 1; Rl ~ 5 CH3C~O~)-CO-; R2 ~s CF13: each of R3 and R5 ~8 -COO~, R~
i8 -CONEICl~2COO~l and R6 i3 ~. 5aid compound oP fonnula (A) i~ Icnown as FR-156.
l ~he novel i~munost~mulant~ of the pr~ent invention are of the formula O
Rl ~ H \ D / CO~R4 I n ~CR2)2CON~IHC R3 ' and a pharmaceutically acceptable base salt thereof, wherein R1 is cycloalkyl of four to seven carbon atoms, alkyl of two to ten carbon atoms or cycloalkylmethyl of six to eight carbon atoms; R2 is hydrogen; and R3 is an amino acid residue of the formula D
where X is hydrogen, alkyl of one to two carbon atoms or hydroxymethyl and n i8 an integes o O to 4; and R~ and RS ar~ each hydrogen, alkyl of one to ~ix carbon atoms, cycloalkylmethyl of ~ix to eight carbon atoms or benzyl.
'' ,~", - ,-.: .: ::..:
. .,, ., :;
. ., ,~ , . -: .:
~ 7~
; , -4- :
:
A preferred group of compounds are those where Rl ~
is alkyl of five to eight carbon atoms, R2 is hydrogen, R3 is said amino a~id residue where X, n and R5 are as defi~ed, and R4 is hydrogen. Particularly pref~rr~d are S those compounds within the group whexe n i3 0 and R5 is hydrogen, said alkyl or ~yclohexylmethyl. Especially pre~erred compounds are those wherein Rl i~ (R~S) 2-ethyl-1-butyl, R~ is hydrcgen and ~ i~ methyl, Rl i8 ~R,S~ 3-heptyl, R5 is hydrogen and X is methyl, Rl is ~R,S) 2-methyl-1-pentyl, R5 is hyarogen and X ~g methyl, Rl is (R,S) 2-heptyl, R5 is hydrogen and X is methyl, R~
is ~R,S) 2-ethyl-1-pentyl, R5 is hydrogen and X is methyl, Rl is (R,S) l-hexyl, R5 is hydrogen and X is IS methyl, Rl is ~R,S) 2-ethyl-1-he~yl, R5 i8 hydrogen and X is methyl, Rl is (S) or ~R,S) 2-methyl-1-hexyl, R5 is hydrogen and X is methyl, Rl is IS) or (~,S) 2-ethyl-l-hexyl, X is methyl and R5 is hydrogen and Rl is l-hexyl, X is methyl and R5 is ~ydrogen. Also espe-cially preferred is the compound wherein Rl is l-hPxyl, X is hydrogen and n i~ 3. Especially pr~ferred esters are those wherein Rl is (R,S) 2-ethyl-1-pe~yl, X is methyl and R5 is n-butyl, i_butyl or cycloh~xylmethyl, R1 is (S) or (R~S) 2-methyl-1-hexyl, X i5 methyl and R5 is n-butyl, i-butyl or cyclohexy}methyl and Rl is ~S3 or (R~S3 2-ethyl-1-he~yl, X is ~ethyl and R5 is n-butyl, i-butyl or cyclohe~ylmethyl.
R ~econd preferred group of compounds are tho~e wherein R1 is cycloalkyl of four to ~even carbon atoms, R2 is hydrogen and R3 is said amino acid residue where n ~-: is 0, X is alkyl o~ one to two c~r~on atoms and R~ and R5 are each hydrogen. E~pe~ially preferred within ~his group is the compo~nd ~here R~ is ~yclohexyl and X is methyl.
.
~2~3~7t~4 A thixd preferred ~roup of compound$-are those wherein Rl is alkyl of five to eight car~on atoms, R2 is hydrogen, R3 is ~ai~ ~mino ~cid residue where X is hydrogen or alkyl o~ one ~o two carbon atoms, n i8 an integer of O to 4 and R5 is hydrogen and R4 is ~lkyl o~
one to 5iX carbon atoms, c~cloalkylmethyl of six to eight carbon atoms or benzyl.
The present invention is al~o directed to ~ pharma~
ceutical composition in unit do~age form comprising a phar~aceutically acceptable carrier and a~ antiin~ect~ve or i~muno~timulant effective amount of a compound of formula 1 and a use for trea-ting an inEection in a human ~uffering therefrom of a comound of formula 1.
1~
~ y pharmaceutically acceptable base salts o~ said compounds of formula 1 is meant salts ~ith inorganic or organic bases such as alkali metal and al~aline earth 2D metal hydroxides, ammonia, triethylamine, ethanolamine and ~i~yclohexylamine.
The configuration of the amino acid moieties which make up the compounds of formula 1 is ~ignificant as regards the pharmcological activity of said compounds.
The mo~t potent act~vity is observed in the compounds o~
for~ula 1 having the ~ter~ochemi~try indicated ~n ~aid for~ula. In those compounds of formula 1 where R2 a~d X
are other than hydrogen, the preferred stereochemi~try at said carbon is indicated as L and ~, respectively.
Also considered within the scope o~ the pre~ent invention are compounds of formula 1 where R3 is al~oxy, cycloalkoxy, aralkoxy or alkoxy substituted by one or more of the substituents ~elected from amino, dial~yl-ami~o, hydroxy, alkoxy and halo.
57~
- 5a - 72222-8 The present invention is further directed to a process or preparing a compound of formula (1) or a pharmaceutically acceptable base salt thereof. This process comprises:
[A] acylating the amino group in a peptide of the formula:
H2N ~
CH
I (L) o (CH2)2CONHCH~C-R3 (wherein R2, R3 and R4 are as defined above except that if they interfere the reaction, they may be blocked by a blocking group), with a carboxylic acid of the formula:
RlCOOH (4) (wherein Rl is as defined above) or an activated derivative thereof, followed by, if necessary, the removal of the blocking group, or [B] amidating the carboxyl group or the activated form thereof in a compound o the formula:
~; ~
7~
- Sb - 72222-8 RlCONH (D) / C2R4 fH ~L) (5) (CH2)2CONHCH-CO~OH
(wherein Rl, R2 and R4 are as de~fined above except that if they interere the reaction they may be blocked by a blocking group, and the carboxyl group attached to the group -CHR2- may be in an activated form), with an amino compound of the formula:
(D) 2 1 ( 2)nC~R5 (6) X
(wherein X, n and R5 are as defined above except that if they interfere the reaction, they may be blocked by a blocking group), followed by, if necessary, the removal of the blocking agent, thereby producing a compound, or [C] amidating a D-glutamic acid derivative of the ormula:
- 5c - 72222-8 l RlCNH (D) / C2R4 CH (7) I
( 2)2 (wherein Rl and R4 are as defined above except that if they interfere the reaction, they may be blocked by a blocking group), or an activated derivative thereof, with an amino compound of the formula:
(L) H2N-CHCOR3 (8) (wherein R2 and R3 are as defined above, except that if they interfere the reaction, they may be blocked by a blocking group), followed by, if nece~sary, the removal of the blocking group, and [D] if required, converting a compound (1) produced by any one of processes [A] through [C] into a pharmaceutically : acceptable base salt thereof.
, . . .
~,~f~
.
T~e compounds of for~ula 1 ~re prepared by a~y of several methods known to tho~e skilled in th~ art. ~he methodology involves the fo~tion of peptid~ linkages be~ween amino acid~ which, b~cause of their ami~o and carboxy groups, a~d ~requently the prese~ce o~ other reactive groups, necessitate the protection of said groups and/or the activation o~ such groups, particularly the carboxy group, in order ~o achieve a certain reaction or to optimize ~uch a reaction.
P~efef~b/~
n-ge~ two routes are employed in the synthesis of the compounds o~ fonmula 1. The first procedure ut lizes the coupling of the fragment RlCN~ ~ D / C02R~
7~ L :~
(C~2~2co~ aco2~
R~ :
with the a~ino acid fragment D
~2NCH (~ H2) nC02R5 2S The seco~d procedure co~rises acylation of the p~ptide ~2N \ D C02~4 C~ L O
- 30 1 ~
~2~2CON~ R3 ;' with ~he appropriate acid RlCO2~, In the exa~ples presented herein, cert~in protecting and activating groups are speci~iGally illustrated. ~owever, o~e skilled in the ~rt will S recognize that other protecti:ng or activ~ting groups could have been used. The choic~ of a particular protecting group is dependent to a gre~t extent UpOD the availability of the necessary reagent, i~s e~fect upon solubility of the ~protected~ compound, its ease of removal and the presence of other groups which might be effected by its use; i.e., its selectivi~y, ox its removal.
For example, it will be necessa~y, or at least desirable, in many reactions to protect the amino groups and/or the carboxy groups. The synthe~ic route chosen for the peptide synthesis may require removal of one or the other or both of said protecting groups in order to permit further reaction at t~e regenerated ami~o or carboxy group: i.e., the protecting groups used are reversible and, i~ ~ost instances, ar~ removable independently of each otherO Additio~ally, the choic~
of protecting group for a giv n amino group depends ~pon the xole of said amino group in the overall reaction schemen Amino protecting groups having varying levels of lability, iOe.; eas~ of removal~ will be used. The sam~ is true as regards car~oxy protecting yroups. Such groups are k~own in the art and atte~tion is dlrected to the re~iews by ~odansky et al., ~Peptide Synthesis~, 2nd : 30 Ed., John Wiley ~ Sons, ~.Y. (197~ reene, "Protective Groups in Organic Synthesis~ John ~iley ~ Sons, (l98l~; ~cCmie, WProtective ~roups in Organic Chemistry~, Plenum Press, N.Y. (1973~; and to Sheppard in ~Comprehensive Organic Chemistry, The Synthesis and j....
j:
- 8 ~ ~ 78~ 72222-8 R~actions of Organic Compounds", Pergaman Press, N.Y. (1979), edited by E. Haslam, Part 23.6, pages 321-339.
Conventional amino and carboxy protecting groups are known to those skilled in the art. Representative amino protect-ing groups, but by no means limiting thereof, are the following:
such as benzyloxycarbonyl, substituted or unsubstituted aralkyl such as benzyl, trityl, benzhydryl and 4-nitrobenzyl; benzylidene;
arylthio such as phenylthio, niotrophenylthio and trichlorophenyl-thio; phosphoryl derivatives such as dimethylphosphoryl and O,O-dibenzylphosphoryl; trialkylsilyl derivative~ such as trimethyl-silyl; and others as are described in U.S. Patent 4,322,341. The preferred amino protecting group is benzyloxycarbonyl. Procedures for substituting said group on a given amino group are well known, In general they comprise acyla-ting the appropriate amino compound with benzyloxycarbonyl chloride (benzylchloroformate) in a reaction-inert solvent, e.g., water, methylene chloride, tetra-hydrofuran, in the presence of a base (acid acceptor) e.g., sodium or potassium hydroxide when water is solvent; and, when an organic solvent is used, in the presence of a tertiary amine such as Cl_4 trialkylamines and pyridine. When an aqueous solvent system is used the pH of the reaction is held at about pH 8-10, and prefer-ably at pH 9. Alternatively, when the reactant; i.e., the com-pound, an amino group of which is to be protected, contains 'basic groups, it can serve as acid acceptor.
The acyl group, RlCO is introduced into the peptide by standard acylation procedures as by reacting said peptide with the ~r 9 - ~2~57~ 72222-8 appropriate acicl chloride or bromide in a reaction inert solvent.
Favored conditions are non-aqueous conditions including addition of a suitable base such as an organic base, preferably a tertiary amine such as triethylamine, N-methylmorpholine or pyridine. The preferred solvent is methylene chloride.
Representative carboxy protecting groups are various esters such as silyl esters, including trialkyl silyl esters, trihalosilyl es-ters and haloalkylsilyl esters; certain hydrocarbyl esters such as Cl_4 alkyl, espeeially t-butyl groups, benzyl and substituted benzyl esters, benzhydryl and trityl; phenacyl and phthalimidomethyl esters; certain substituted hydroearbyl esters sueh as chloromethyl, 2,2,2-trichloroethyl, cyanomethyl; tetra-hydropyranyl; methoxymethyl; methylthiomethyl; protected earbazoyl sueh as -CONH-NHR wherein R is an amino protecting group as disclosed above, espeeially benzyloxy earbonyl; and others as are described in U.S. Patent 4,322,341. A highly favored carboxy protecting group is the t-butoxycarbonyl group.
The protected amino and carboxy groups are converted to the unprotected amino and earboxy groups by proeedures known to those skilled in the`art. The benzyl group, the preferred pro-teeting groups for earboxy (as part of the proteeted carbazoyl group) groups are removed by eatalytie hydrogenation over palla-dium, especially palladium-on-earbon. Alternatively, said pro-teeting groups are removed by means o trifluoromethanesulfonie acid in trifluoroaeetie acid and in the presenee of anisole to suppress alkylation. The t-butoxyearbonyl group is readily remov-ed by treatment with dioxane saturated with hydrogen chloride.
-:~2~
-ln- ' Activation of carboxy groups as a menas of expe~ -diti~g a given reaction is methodology known to those skilled in the art~ Esp~cially use~ul in the ~erein described reaction ~equence are the ~5~ of anhydrid~s, S particularly cyclic anhydride~; and activated esters, such as those derived from N-hydroxyphthalimide and N-hydroxysuccinimide, ~oth of which are used in peptide syntheses.
The activated N-hydroxysuccinimide ester~ expedi~e subsequent reactions at said activated ester groups. As the skilled artisan will recognize other activating groups could be used. A group of particular i~terest i5 the N-hydroxyphthalimido group, which group is u~ed in the same manner as is the N-hydkoxysuccinimido group.
In both instances, a dehydrative ~oupling agent is used to form the activated ester. Representative of such coupling agents a~e l-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide ~bod-p-toluene sulfonate, dicyclohexyl car~odiimide, N,~'-carbonyldiLmida~ole, N-~3-dimethyl-aminopropyll-N'-ethylcar~odiimide hydrochloride, ethoxy-acetylene, diphenyl~etene and ~-ethyl-S-phenylisoxa-zolene-3'-sulfonate. The reaction conditions for using such coupling agents are well described in the litera 2s ture. In general they comprise the use of a react on-inert solvent and te~pera~ures ranging ~rom ~mbient to 100C. The above-mentioned carbodiimide reagents are favored since they penmit use of ambient reactiGn temperature and afford satisfac~ory yields of the 3n desired esters.
Upon completion of the coupling reactions leading to the final products, the variou~ protecting groups can be r~moved by t~e appropriate t~ch~iques previously discussed, and the compo~lnds of fonmula 1 isolated.
~ 5~1~34 :' ::, ~ he pharmaceutically acceptable base-salts of formula 1 compounds, where R3 is hydroxy or R4 or R5 is hydrogen, are obtained by treating a solution, preferably aqueous ~olution, thereof with ~ b ~e such a~
are enumerated above, generally in ~toichiom~tric proportions. The ~alt~ are isolated by evaporation or by precipitation.
The products of this invention are useful ~8 agents in mammals, including humans, for the cli~ical and therapeutic treatment of diseases caused by various patho~enic microorga~isms, especially gram-negative ~acteria. They are also use~ul as immu~o~tLnulants in mammals, including humans, having an inc~ease risk of infection due to existing or i~creasad risk of infection due to existinq or clinical~y-induced Lmmunosuppression.
The test procedure, which used C3H/HeN ~le mice from the Charles River Breeding Laboratory, is presented below. The mice wer~ acclimatized for 5 days before use and then treated either subcutaneou~ly ~SC) or orally ~P0) with various dilution~ ~100, 10, 1 and 0.1 mg/kg) of the test compound or place~o (pyrogen ~ree saline) usin~ a volume of 0.2 ml. The treatment re~iment was dependent on the infec~ious organism utilized: -24 and :
O hours before challenge for Rlebsiella ~ne~moniae in normal ~ice; and -3, -2 and -1 day before challenge for Escherichia coli or Sta~. aureus in immunocompromised mice. Challenge ~as administere~ intramuscul~rly ~IMl in the hip in the case of R. pneumoniae or intr~-peritoneally (IP) in t~e case of S. coli and ~Q~.
aureus. A volume of 0.2 ml. was u~ed for the challenge.
~ortality was recorded after 7 days in the case of R.
pneumoniae and after 3 days i~ ~he case of the other two microorganism challenges.
~lture Preparation: -K. pneumoniae, E. coli, or ~ . 2ureu~: theculture was ~treaked for purity fro~ fro~n blood st~ck on brain heart infusion (~I) agar. ~hree colonies w~re picked from the 18 hour plate culture and placed into 9 ml. of BHI broth~ The broth culture was grown for 2 hours at 37~Co on a rotary shaker after which 0.2 ml.
was streaked on the surface of several B%I agar slants.
Pollo~ing an 18 hour incubation at 37C., the slants lo were washed with B8I broth, the cultur~ density adjusted using a spectronic 20 and the appropriate dilution made to achie~ an LD90 challenge level in nor~al mice.
When used as antiinfective or immunos~imulan~
agents in humans, the compounds of thi~ invention are ,, conveniently admini~tered via the oral, subcutaneous, intramuscular, intravenous or intraperitoneal routes, generally in composition form. Such compositions include a pharmaceutical practice. For axample, they can be administered in the form of tablets, pills, powders or granules co~tai~ing such excipie~ts as starch, ~ilk su~ar, certain types of clay, etc. They can be administered in capsules, in admixtures with the same or equivalent excipients. They can also be administered in the form of oral suspensions, solutions, ~mulsions, syrups and elixirs whicb ~ay contain flavoring and colo~ing agents. For oral administration of the therapeutic agents of this invention, tablets or capsules containing from about 50 to about 500 mg. are suitable for most applica~ions.
The physician will determina the do~age which will be most suitable for an individual pa~ient and it will Yary ~ith the age, weight ~nd response of the particular patient and the ro~tP of a~ministratio~. The favore~
~2 ~ ~'7~ --13- ..
oral ~osage range, in single or divided do~es, is from about 1~0 to about 300 ~g/kg/clay. The favored parenteral dose is from a~out 1~0 to about 100 mg/kg/day: the preferred range from about 1.0 tc ~bout 2û n~g/kg/day. :
This invention also provided phar~aceutical compositions, including u~it dosage for~s, valuable for the use of the herein descri~ed co~pounds for the utilities disclosed herein~ The dosage form can be given in single or multiple doses, as previously not~d, to achieve the ~aily dosage effective or a particular utility.
The following examples are provided &olely ~or the purpose of further illustration. In the intere~t of brevity, the following abbreviations for pea~ shapes in the NMR spectra are used: s, ~inglet; d, doublet; t, triplet; q, quartet; m, multiplet. The terms mole and millimole are abbreviated as m and mm, respectively.
' '' "
;7~
' ;;:' . .
N-~eptanoyl-D-gæ~a-glutamyl-glycyl-D-alani~e (Rl S C~3(C~2)5; R~ DC~C~IcD
lA. N-heptansyl-D-gamma~glUt~myl(alpha benzyl ester)-~lyci~e ':
., To a solution o~ B97 mg. (13.0 ~m~ o~ glycine and 1.3 g. (13.0 mm) of triethyla~ine in 10 ~l. of water was added 5.0 g. ~11.2 mm1 of N-heptanoyl-Dogamma glutamyl 0 (alpha benzyl ester)-hydroxysuccinimi~e e~ter in lon ~1.
of dioxane, and the resulti~g reaction mixture allowed to stir at roo~ temperature for 80 ho~rsO ~h~ solution was poured into 300 ml. of ethyl acetate and the -~
separated organic pha~e washed wi~h 10~ hydrochlorac acid, water and a brine solution. The organic phase was separated, dried over magnesium sulfate and concentrated in vacuo to dryness. The residue ~a~ trituxated with _ ~ .
diethyl ether and filtered under x~itrogen, 3.43 g. q74~ -yield).
lB. N-heptanoYl-D~ qlutamy To a solution o~ 2 . O g. ~4 . 78 ~i of N -heptanoyl-D-gan~na-glutamyl (alpha benzyl ester)-~yci~e, 1.75 g. l5 ;
mm) of D-alanine benzyl ester ~-toluenesulfonic acid salt, 506 mg. I5 mm) of triethylamine and 675 mg. ~5 mmJ
of l--hydroxybenzotriazole in 100 ml. sf ~etrahydrofuran -~
was added 3.03 ~. (7.17 m~) of 1-cy~lohexyl-3~(2- :
morpholinoethyl)~arbodiimide metho-~-toluenesulfonate ::
and the reaction mixture stirred at room temperature ~or 18 hours. The reaction mixture was poured i~to 300 ml.
of ethyl acetate and the organic phase separated and was~ed wi~h 10% hydrochloric aeid, water, a saturated sodium bicarbonate 601ution and a brine ~olutio~. The organic phase was separated, dried ov~r magnesium , - :~L2 sulfate and concentrated in vacuo. The residue was triturated with diethyl ether and filtered under nitro-gen, 2.7 g. Two grams of the solid i~ 75 ml. of methanol with 403 mg. of lO~ palladium hydroxide on 5 charcoal was sha~en in a hydrogen atmo~phere at an initial pressure o 50 p~i for 4 hours. The catalyst was filtered and the filtrate evaporated u~der reduced pressure and the residue was clissolved in water and lyophilizad to give 1.23 g. (5~0% yield) o~ the deæired product as a white ~olid.
The NMR spectrum ~DMSO-d6) 3howed absorption at 4.35-4.2 (m, 2~)~ 3.83 (~, 2H), 2.35 (t, J~7Hz, 2B), 2.17 ~t, J-7~, 2H), 2.1-1.8 (m, 2~), 1.55-1.45 (m, 2H), 1.3 (d, J=6~z, 3~1, 1.17 (bs, 6H) and 0.75 (bs, 3~) ppm.
N-Hep~a~oyl-D-gamma-glutamyl-glycine (Rl = CR3~CY2)5;
R~ - ~; and R3 ~ O~) A solution containing 1~0 g. of ~-heptanoyl-D- :~
gamma-glutamyl (alpha benzyl ester)-glycine in 50 ml. of methanol was treated with 100 mg. of lO~ palladium hydroxide on charcoal and shaken i~ a hydro~en atmosphere at 50 psi for 3 hoursO The catalyst was filtered and the filtrate concentrated in vacuo, The residue was dissolved in hot water and ~vaporated i~
vacuo. The residue was redi~solved in water and lyophilized to give 630 mg. (83~ yield3 of the desired product as a white solid.
The NMR spectrum (D~SO-d6) showed absorption at 4.37-4.25 (~, 1~)~ 3.9 (s, 2~), 2.35 (t, J=7~z, ~
2.18 (t, J=6~z, 2~), 2.~-1.8 (m9 2~), 1.5-1.4 ~, 2H3, 1. 8 (~s, 6~) and O. 7 (bt, 3~) ppm. . .
EX~PLE 3 - .
N-~eptanoyl-D-ga~ma-glutamyl-qlycyl-glycin~
(~1 = CH3(ch23s; R2 ~ ~; and R3 2 2 3A. ~-heptanoyl-D-gamma-gluta~yl (alpha benzyl ester)-glYci~e hydroxysuccinamide ester To a cold 501UtiOII (0C.1 o~E 13rO g~ (31 mm) c~f N~heptanoyl-D-gamma-glutamyl (alpha ~enzyl est~r~ :
glycine and 3.91 g. (34 mm) o~ N-hydroxy~uc~in~mide in 400 ml. of ~e~rahydrofuran was added 7.0 g. (34 mm) oP
dicyclohexylcarbodii~ide, and the mi~ture ~llowed to stir at 0C. ~or on~ hour and at roo~ t~p~rature ~or 18 hours. The solids were filtered and the filtxate concentrated under reduced pre~sure. The residue was triturated with diethyl ether and filtered ~nd~r nitro-gen to give 15.4 g. (98~) of the desired intermediate.
3~. ~
To 2.0 g. (3.97 mm) of N-heptanoyl-D-gamma-glutamyl .
(alpha benzyl es~er)-glycine hydroxysuccinamide ester in ~o 100 ml. of dioxane was added ~46 ~g. 15.95 mm) of glycine and 0.55 ~1. l3~9 mm) of tr~ethylamine in 10 ~1. ', of water, and the resulting reactisn mixture allowed to stir at room temperatur~ for 13 hours. The 6~1ution was poured into 100 ml. of ethyl acetate and the organic ~.
layer washed with 2.5~ hydrochloric acid, water and a : brine solution. The organic layer was separa~ed, dried ~::
over magne~ium sulfate and concentrated to drynessO The '.
residue was triturated with diethyl ether and filtered under ni rogen to give 1.7 g. of white ~olid. One and 30 iive-t~nths grams of the solid in 75 ml.. of methanol containing ~00 mg. o~ lOS palladium hydroxide on carbon ! ~:
was shaken in a hydrogen atmosphere at 50 psi or 3 ~.
hours. The catalyst wa~ filtered and the filtrate '~
!
' .~.
' j`'.''''.
I':
i~
',:.
~57~3~
concentrated ~n vacuo. The residue w~s dissolved in water and lyophilize~ to give 1.12 g. ~90~ yie~d) of the desire~ product.
The NMR spectr~m ID~SO-d6) $howed ab~orption 8.2-8.0 (m, 3~), 4.19 ~m, 1~ .8-4.6 tm, ~), 2.25 ~t, J-7~z~ 2H), 2.1 (t, J-6Hz, 2~), 2.05-1.7 (~, 2~), 1.5 (m, 2~), 1.25 5bs, 6~) and o.as ~t, JY68Z~ 3H3 ppm.
EXAMPI~ 4 N-HeptanoyloD gamma-gluta~yl-glycyl-D~serine D
1 3(C~2)5; R2 ~ ~; and R3 ~ -N~CHtC~2O~)CO2~) ~ .
Starting with 2.0 g. (3.98 mm) of h-hep anoyl-D-g~mma-gl~tamyl (alpha benzyl ester)-glycine hydroxy~
succinamide e~ter, 780 mg. ~4.02 ~m) of O-benzyl-D-serine and .556 ml. (4.02 mm3 of triethylamine and following the procedure of Example 3B, 902 ~g. ~76%
yi~ld1 of the desired product i~ i~olated, m.p. 130- :~
132C.
The NMR spect~um (DMSO-d6) ~howed a~sorption at 8.36-7.94 ~, 3~t~ 4.~6-4.28 l~, lR)~ ~.28-4.08 (~, lH), 3.94-3.50 ~m, 4~I); 2.25 ~t, J~91~z, 2EI1. 2.17 It, J=g}~z, lH)~ 2.10-1.04 (~, 14~) and 0.9 (t, J=6~z, 3~ ppm.
EXA~IPL.E 5 ~-Heptanoyl-D-gamma-glutamyl-glycyl-D alpha-æminobutyric acid ~Rl ~ C~3(CH2)5 ; 2 R3- -N~C~(C~2C~33C02H
The procedure of ~xa~ple 3B was repeated, ~tar~ing with 2.0 g. (3.98 ~m) of N-heptanoyl-D-gamma-glutamyl (alpha benzyl ester)-glycyl hydroxysuccinamide ester, :~
400 mgO S4.02 mm) of D alpha-~minobutyric acia and .55S
ml. i4~02 m~ of triethylamine, to give 632 mg. (57%
yield) of the desired proauct, m.p. 140-141C.
. .
-lB-.
The NMR spectrum ~DMSO d6) showed absorption at 8.16-8.04 (m, 3~), 4.22-4.0B ~m, 2~, 3.8~-3.58 (m, 2~), 2.2 It, J~9~z, 2H~, 2.12 (t, J-g~z, 2~ .D4~1.0 ~m, 15~) and 0.35 ~, J~6~z, 6~1 ppm.
E ~ ]~ 6 N-~eptanoyl-D-gamma-glutamyl-glycyl-3-am~nopropionic 1 3~ 2~; R2 ~; and R3 - -N~(CH2)2C02H) Following the procedur~ of Example 3B and ~tarting with 1.5 g. (3.0 mm) of N-heptanoyl-D-gam~a-glutamyl ~alpha benzyl ester)-glycine hydroxysuccinia~ide ester, 350 mg. ~3.9 mm) of 3-aminopropionic acid and 055 ml.
(3.9 m~) of triethylamine, 500 mg. ~43~ yield) of the desired product was obtained, m.p. 135-138C.
The MMR spectrum ~DMS0-d6~ sh~wed absorption at 8.19-3.02 lm, 2~), 7.98-7.87 It, JG5Hz, 1~), 4.25-~.1 (m, 2~), 3.8-3049 (m, 2~), 3.44-3.1 (m, 2~), 2.4 (t, J36Hz, 2~), 2.22 (t, J=7~z, 2~), 2.14 ~t~ J-7~z, 2H), 2.1-1.67 tm, 2~), 1.6-1.17 (m, 8~) and 0.88 (t, J=6Hz, 3~ ppm.
~X~MPLE 7 ~-Heptanoyl-D-gamma-glutamyl-glycyl-~-aminobutyric ~1 C~35~H2~5 ; R2 s ~; and R3 ~ -N~CH~) C0 ~) .
The procedure of Example 6 wa~ r~peated ~ubsti-tuting 410 mg. (4.0 mm3 of 4-aminobutyric acid for the 3-aminopropionic acid to giYe 600 mg. l~o~ yield) of the desired produc~, m.p. 140-1~2C.
The N~R spect~um ~DMSOod6J ~howed ab~orption at 8.18-8.03 Im, 2~), 7.88 (bt, J=4~z, lB), 4.17-4.09 (m, 3~ 2B~, 3.81-3.48 (m, 2~, 2.32-2.0~ ~m, 6~)~ 2.0B-1908 Im, 12~) and 0.88 It~ J=6~z, 3~) ppm.
, --19~
~ ~ LE 8 N-~eptanoyl-D-gamma-glutamyi-gly~yl-5-amino-pentanoic acid (Rl ~CH (C~ ~ -; R2 ~ ~; and Sub5tituting 470 mg. (4.0 mm~ ~ 5-~inopentanoic acid ~or 3-aminobu~yric acid ,and ~oll~wing the proc~du~e of Example 6, 520 mg. ~423 yileld~ of the desir~d product was obtained, m.p. 122-124~C.
The NMR spectrum (DMSO-d6~ ~howed absorption at I0 8.25-7.9~ ~m, 2~), 7.85 It, J~5Hzt 1~), 4.25~.1 (m, 2~), 3.8~-3.46 (m, 2H), 3~24-2.9 (m, 2~), 2.21-2.08 (m, 6~), 2.08-1.2 (m, 14H1 and 0.88 ~t, J86~Z, 3~) ppm.
N-Heptanoyl-D-~amma-glutamylglycyl-6-aminohexanoic IS ~Rl ~3(C~2)5 ; ~2 H; and R3 ~ -N~(CH2~5C0 ~ .
The procedure of Example 6 wa~ again repea~ed, substituting s3n mg. ~4.0 mm) of 6~ ohexanoic acid for the 3-æminobutyric acid to give 520 mg~ S40~ yi~ld) of the desired produ~t as ~ whit~ foamO
The NMR spectrum (DMSO-d6) showed absorption at 8.28-7.9 (m, 2~), 7.82 (bt, J=4~z, 1~, 4.27-~.1 tm, 2~), 3.81-3.47 (mt 2~), 3.15-~.9~ ~m~ 2R), 2.3-2.08 (m~
6~), 2.08-1.18 (m, 16H) and 0.88 St, J~6~z, 3~) ppm.
N-Iso~aleryl-D-gamma-glutamyl-glycyl-D-alanine (~1 (C~332c~2-; ~2 ~ ~; and ~3 ~ CHIC~3lco~
lOA. ~
To a cold (0C.) solution of 100 ml. methylene chloride contai~ing 10 gO (57 ~m~ of ~-t-butyloxy-carbonylglycine, 20 g. ~57 mm) of D-alanine benzyl ester ~-toluenesulfonic acid salt and 5.77 g. ~57 ~) of ~5;7~3~
: ~ triethylamine was added 12.3 g. t60 ~) of dicyclohexyl-carbodiLmide and the resulting ~eaetion ~ixture allowed to warm to room temperature. After 18 hours the ~ixture was filtered and the filtrate ¢oncentrat~d in ~acuo.
The residue was disFolved in 200 ml. of e~hyl acetate and the organic layer washed with 2.5~ hydrochloric acid, water, a saturated sodium bicarbonate solution and a brine ~olution. The organic layer ~a~ ~eparated, ' dried over magnesium sul~ate and evaporated under r~duced pressure. To the resulti~g oil ZOO ~1. of dioxane saturated with hydrogen chloride was added.
A~ter 30 minutes 400 ml. of diethyl ether was added and the product filtered under nitrogen, 10.9 g. (70% .
lS yield).
lOB. N-t-butoxycarbonyl-D-gamma-glutamyl ~alpha benzyl ester)-kr~ ~n~m~ ester h yd ~ ~ lsu cc In C h~ t ~
~o 1500 ml~ of methylene chloride containing 50 g. .
(143 mmJ of N-t-butoxycarbonyl-D-gam~a glutamic acid alpha-benzyl e~ter and 17.3 g. ~150 ~ml of ~-hydroxy-succinamide was added 30.9 g. (15 mm) of aicyclohexyl-carbodii~ide and the resulting reaction ~ixtura allowed to stir at room temperature for 18 hours. The ~olids were filtered and the filtrate concentrat~d in vacuo.
The residue was triturated with diethyl ether and the :
~slids filtered l-nder nitrogen, 43.7 g. ~68% yield~
lOC. D-qamma-glutamyl (alpha benzyl esterl-glycyl-C~
:.' A solution containing 4~3 gO ~9.45 mmj of N-t-butoxycar~onyl-D-gamma-ylutamyl (~lpha benzyl ester) hydroxysuccinamide e~iter, 2471 g. (9.92 ~) of glycyl~
~-alanine ben~yl ester hydrochloride and 1.0 g. i9.92 mM) of triethyl~il~e in 100 ml. of ~ethylene chlor~de -~g~7~3~
was allowed to stir at room temperature for 18 hours, and was then concentrated in ~vacuo. Th~ residue was dissolved in 200 ml. of ethyl ~cetate and the ~olution washed with 2.5~ hydrochloric acid, ~ater, 10~ pota~siuM
carbonate and a brine ~olution. The organic pha~e was separated, dried over ~aqnesi~m sulfate and e~apor~ted under reduced pressure. The :residue was treated with 200 ml. of dioxane ~aturated with hydroge~ chloride and allowed to stir for 2 ho~rs. ~he soluti~n was concen- -trated to dryness in vacuo and the r~idue triturated ~ . ~
with diethyl e~her. The solids were ~iltered under nitrogen, 3.41 g. (73~ yield~.
lOD. -isovaleryl-D-gamma-gluta~y~ ycyl-D---alanine 15To a solution of l.0 g. (2~03 ~m) o~ D-gamma-glutamyl (alpha benzyl ester~-glycyl-D alanine benzyl ester hydrochloride and 616 mg. (6.09 mm) of triethyl-amine in 50 ml. of methylene chloride ~as added 490 mg.
14.06 mm~ of i~o-valeryl chloride ~nd the reaction 2D mixture stirred at room temperatur~ for 80 hours. ThQ
methylene chloride ~as evapoxated in vacuo and the residue dis~olved in ethyl acetate. The re~ulting solution was washed ~ith 2.5% hydrochloric acid, water 10~ potassium carbonate, water, and a brine solution. ..
The organic phase was separated, dried over magnesium sulfate and concentrated under vacuu~. ~he residue was triturated with die~hyl ether, iltered under nitrogen (910 mg.) a~d 700 ~g. dissolved in 50 ml. of mstha~ol.
Palladium hydroxide 200 mg. was added o the solution and the mixture shaken in a hydroge~ atmosphere at 50 psi ~or 3 hoursO The cataly~t was filtered and the sol~ent re~oved in vacuo. The re~id~e ~as dissolved in water and lyophilized to give 36~ ~g. t~5~ yield~ of the de~ired pr~duct~
7~3~
The ~MR spectrum (DMSO-d6) showed ab~orption at 8.25-8.05 (m, 3~), 4.33-4.12 (m, 2~), 3.72 ~d, Js6~z, 2~), 2.21 (t, J-8~z, 2~), 1.88--1.68 (m, 1~), 2~0801.9 (m, 4~1, 1.28 (d, J~9~z, 3~) alld 0.~ ld, J~7~z, 6~) ppm.
Starti~g with the appropr:iate acid chloride and D-gamma-glutamyl ~alpha benzyl ester)-glycyl-D-alanine benzyl ester hydroehloride and employi~g the procedure of Exæmple lOD, the following compounds were pr~pared:
O .
RlCN~ \ / 2~
I D
~C~12) ;2CON~CH2CON~IIHS:0 ~3 '-:
R
~0 CH3(Ca2)8- >l~S I~SO-d~ 8.22 ~, 2h), 4.35-~13 tm, 2H), 3.83 (d, J-6~z, 2~3~ 2,22 ~t, 2~ 3~6~z, 2~), 2.1~ (t, J~6~z~ 2~) D 2.08-l.~S ~ 5 1~18 (~, 17~) and O.gO ~t~ J~7~z, 3H) -3.57~
:
Rl m. p . ~ ~ ~
(CH3) 2C}~- ~110 (dec) (D~5O~a6) 8 . 2-8 . 08 (~, 21~J, 8.02 (d, J- .
S g~æ, 1~ . 3-4 .1 (m, 2~), 3.72 Id, J~7~z, 2~), 2.53-2.40 lm, 1~), 2.~2 ~t, J~g~z, :
2~), 2.,10-1.7 (m, 2~) " 1.~8 ~d, J~9~z, 3~) ~Ad 1.08-0.9 .. (In, 6~) '~
~ ~110ld0c~ d6) B.3-7.9 (mt 3~), 4 . 33-4 ~ 17 (m, 2~), 3 . 72 (d , J~5~z, 2~1, 2 . 3-? .1 ,:~
(~ 2~) and 2 .10-1. 9 . Im, lS~) ;.
CH3(C~2)2- a~110(d2c) (~O-d6) 8.25-8.05 (~, 3~ . 35-4 . 22 :~
(~, 2~), 3.72 ~d, J~
~, 21I), 2.22 ~
.
J~ F~zo ~Pl), 2.12 (to J~ lz, 2~), 2 . 08-1 . 77 (~, 2~1, 1 . 55 .
lq, J~z , 2EI), ~ . 28 .
~d, J~ , 311~ and 0 u 88 (t ~ J~7i~z D 3E~
-'.
~' ' Rl - m.p. ~C.
._ C~3 ~C~2) 4- >190 Ide~3 ~D~S0-d~ .33~8.0 ~, 3E~), 4 . 35~
lla, 2E~), 3.7 5d, J~ -6~z, 2R~, 2.17 (1:, J~B~z, 2~1), 2.1 It~
1~8~ 2t 2~ 2.05- :
1 . 63 (~ 2~), 1 . ~8 ~t, ~7Elz , 2~), 1. 2-1.1 (m, 7E~) and 0 . 85 ~t, JSG7~Z t 3~) CE~3 ~H2)6- >180 5dec) tD~01 ~.42-~28 (m, 211) o 3r91 Is, 1~3, 2 0 4 ~ 7~z , 2131 ~ 2 a ;27 ( 1 ~ J27~1z, 2~), 2.22 1 . 94 Im, ., 2~), 1.65-1,55 (m, 2H) " 1.39 Id, J~8Hz, 3~) t 1.3~-1,17 (m, 8H) and 0 . 73 (m, 3EI) ' ~''~
,.. .
:
~, ' ---25- ;:
,c. 3~E~
~a3 ~3(CH2)3C~c~2~ 6) ~.~7~~-03 ~ , 3~), 4.32~
(~ 2~ ~ 3.72 (d, z , 2E~), 2 . 22 Elz , 2~, 2.27-1 . 68 (m, 6}11, lo 1.42-1~ 0 ~m, 101~1 aad 0 . 94-0 ~ 8 lm. 6EI) ~ 2CH3 15 C~3~C~2)3C~ S0--d~;) 8.22-8~0 ~,S~ ~, 3E~), 4.32~
(~, 2H), 3 . 8-3 . 6 (~, 2~[), 2 . 28-1 ~ 68 SB) " 1.6-1.0 ~, 12~ and 0 . 94O
~.7 tls, 6~.
(C~3C~2)2C~ SO-~6) 8.29~ 7.97 3~), 4 . 33-4 . 1 ~, 2E~3, 3.8~ 3.~9 (~, 2EI~, 2 . 32-1 . 65 ~, 6~) ,, 1.65-1.1?
(~D ~ ~d 1 . 02 -30 0.68 (m, 6~) :: `
'-'.
.
, ~2~7 - R
~C~2-- ~ 50-db ) 8 . 3-B 0 0 (~s 3~), 4 . 32-4 . 1 S ~, 2~), 3.85-3.62 ~3a, 2H), 2.21 ~t, J~8~x ~ 211), 2. 02 8Elz , 2~), 2 . 01-1. 9 (m, lE~
0 1.115 l.S ~r~, 8~, 1,Z8 ~d, J~8Hz, 3~1 a~ 1. 28-0 . 8 (m, SH
(CH3CH2CEI2 ) 2C}ICH~ ~5SO~d6 ) 8 .18-8 . O
n, 3~ . 31~4 . 1 ~ 2~) " 3. 84-3 .
(~ 2E~), 2 0 2~
J~6EI~, 2H) ~ 2.07 ~a, J~BlE~æ , 2H), :~
2,.03~1.7 ~m, 3~), 1 o 15 ~, llE~) ~d O . 87 ~t, J-6Hx, (CH3C~2 ) 2CE~CE12 (D~3S0--d6 ) 8 . 27~7 " 95 ~, 3H), 4 . 3 -~ . 1 (m, 2~3 ~ 307~-3.~ (~, 2~ . 3 1 . 57 (~, 8~), 1..46-1.13 8E~ a~d 0.84 ~t,, J~
8~x, 6 7~4~
:
Rl m. p . ~,~, ~ H3 C~13 (CEI2~ 3C~C~2 5D~SO-d6) 8 .18-8 . O ,:
S ~R, S) ~,, 3~, 4 . 2~--4 . 05 ~m, 2~), 3074-3.S6 ~m, 2H), 2017 5t, J~
9Elz , 2EI), 2 . 12-2 . 0 ~m, lEl), 2 . 0-1 i, 64 lo 5DI~ ~H~, 1 . 2~ (d, J-6~z , 7EI), 1 . 14-O 0 98 ~m, 2EI) ~nd 36Elz, ~) lS
CH3 (CH2) 2CHC~2 (D~IS0 d6~ 8 . 2-8 . 04 (R,S) (mg ~), 4.26-4.08 ~m, 21~, 3 . 76-3 .. 6 (m, 21I), 2 . 28-1 . 64 ~, 7E~ -O.g~
(~, '7~ ~nd 0 . 96-0.7~ ~m, 611).
.
' ~D ~
. .
:,:
:
. .
,~
.
j7~
-~8--Rl al~ ~ ,, H3 ) 2C~ tCH2 ) 3- ~ - 1~S0-d6~ 8 . 2 4-7 . 95 ~s, 3B), ~.3-4.08 l~, 2~1), 3 . ~1-3 0 59 ~Dl, 2~ ,. 21 (t, J-6~z, 2h), 2 0 11 (t, J~z ~ 2~31, ;
2.05 1.38 tm. 7EI) 9 ", l.,Z7 ~d, J~8~z7 3~) ,, 1.17-1.05 ~m, 2 a~ 0.86 ~d, J-lO~z 6~) c~3 (D~SO-d6~ 8 . 24Tr8 . 0 C~3 (CE~ CH- ~, 3H), 4 . 33-4 .11 iR95) ~m, 2~, 3 . 79-3 . 6 (~ 2~ 2 a 41--;! ~ 29 (1;~1, llE~), 2 ~ ;~2 ~t ~
J~8~31z~ 2~ 66 (SD., 2EI) ~ 1~59-1~43 t~ 1038-1~11 ;.
(m, 11~), 1.06-0.95 1~, 3~ and 0 . 87 ,~
~t, ~-6~z,, 3 .
, .
m.p.,~C
C~3C~2 5 CH3(C~23 3CHCH2 ~DI~SO-d6~ 8.24-8.02 (R,S) (m, 3EI), 4.32-4.11 ~m, 2~), 3 ~ 3 . 6 ~t 2~311, 2.24 ~t, ~gt~z , 2~), 2 . 08 : .
Id, J~lz , 2~, 2 . 03-1.,~3 ~ ), 1.4~-1.11 (m, 12~) and 0.97-û.71 ~m, 6H) 15 (CE13)2ClItc~2)~ D~IS0 d~j~ 8.24-8.û~
tm, 3~), 4.28-4.1 i~, 2E~, 3.76-3.6 2E~3, 2 . 18 (~
6~Iz, 2~), 2~1 tt, 6~z , 2~3, ~ O 04- ~ .
1.8~ , 1.84-1.66 (D~ , 1.56-1~38 Im, 3~), 1.23 (~1, 3~6Elz, 311) " 1.2-1, 06 a~, 3~ d ~.82 (~ J~6~{z, 6~) ~
., ::
C
C~3 (C~2) 4~ 2~ fiSO-d6) 3.23 7098 IR~S) ib~9 3J~1), 4,.3-4.13 ~, 2~), 3.~1-3~61 ~Q, 2fl), 2 . 22 (t, -;J5E~ 2~), 2 . 18 - ;:
1.68 ~, 6H), 1.45-1007 ~m, 12~l~ and 0.98-0.8 (m, 6~) .. :
C~3 (C~13)2C~(C~2~2C~c~2 (D~SO-d6) 8.37-8.03 ~
(R,S) l~, 3~I~ O ~.31-4.1 ~, 2H) 7 3.78-3.6 ~El, a~,, 2.26 (t, J~ z, ~, 2.~-1.36 l~, 7E~) ~, 1.3 Id, J~.
8~1z , 5~), 1 . 25~1 . 05 1~" 2}13 ~nd 1. 05-0.73 lm~ 9H) :
~5 :
,~
~ 3~
:
-31- ::~
';
Rl m.~!C. ~ :~
~E~3 ~~ IDMSO~d6) 8.23-7.94 C~3) 2CHC:H2CHC1~2 (Im, 3~1), 4 . 3-~ .13 1~, 2EI), 3.Bl-3.51 (D~, 2~1), 2.21 ~t, J-8Elg, 2~ . 15-2 . 0 t~, 2H), 1 . 9 (t, J~8E~z , 2~1), l . dS-1 . 52 Im, 3EE), 1~ 4--1~ ;!2 (1~1~ 3EIl r 1 ~ 2~2~0 ~ 94 (~ 3~) ~nd 0.94-0.80 (~, 6~
1~ .
C~3C~2 CH3~C}l2~2~HcE~2 IDMso-d6) 8.25-7.9 IR,S~ (m, 3EI~, 4.3~4.0~
Im, 2~), 3.81-3.62 Im, 23~), 2.22 (t, Js8~z ~ 2~1, 2 . 06 (d, J~81~2, 2~
. 02-1 . 89 (m, 1~, 1~ 87-1. 65 lm, 2~
1., 41-1. 06 (~, llH) ~d 0.~8-~.7 (D, 6E~) :
. ~
, ~0 , .
~.
7~
--3~-~1 m ~
O~d2- ~- (DISS0-~16) 8.33-7.9S
1~, 3E~ . 3-~ . ofi (~ 2~) ~ 3.83-3.59 :
~, 2~), 2.21 It, ~:
J~8H;Go 2H), 2.11 l~
3~), 2008-l.a7 ~
1 . 87-1 . 3~ (~, ~B), 1 . 25 ~d, J~88z , ,~
3~ d 1. 22-0. 98 3~
16 (CH3C~2 ) 2C~ ~CR2 ) 2 (1~S0--d6 ) 8 . 21-8 . U
(~ 3~ . 32~
i~n, 2E~), 3.83-3.6 l~, 2~), 2.21 (t J~8~z, 2E~1, 2.11 ~t~ J~ I8, 2H) ~
2~05-1.89 ~m, 1~3, 1.87-1.67 5m, 1~) 0 l.S7-1.38 Im, 3~[3 g 1 . 38Q1. 08 ~m, 9~1 ~d 0.83 It, Jz~6~z~
6Elj , , ' ', .
.
.
~2~7~3~
l ~3 C~3 IC~2) 2CE~ ~C~23 2- ~ ~ DlllSO-d6~ 8 . 24 7 . 97 (R,S) ~3~, 3~), 4033~4.1 (m, 2~), 3 . g6-3 . 59 (~, 2~), 2. 35-2 . 08 ~n, 4~13, 2 . 08 1 . 9 (Dl, 1~), 1 . 89-1 . 67 (~ 1}~), 1.~3-1.46 1 0 416-1 . 02 im, llB~ and 0 0 98-~o73 t~, 6EI) C~3~2 CH3 (C~2l ~,C~C~2 ~S0Wd6~ 8 .2-7 . 94 ;~.
(R,S~ t~, 3E53 0 ~.26~4.06 ~, 2~, 3 . 7~-3 . 56 .
a, 2~[), 2. 16 (t, J1~6~, 2~), 2,.1-l. B4 (~Q, 31~), 1 . 84-1 ,. 6 (~, 2~1), 1 ~ 24 (d, J~
6Bz, 9H), ï.12 0.~2 :;
26 t~, 3~ 9.92~ :
0.64 .- :
~ . ~
- :~ 2~ ;7~
:
C~3 3 (~ ) 5C~C~2~ MSO-d6 ~ 8 . 23-8 . 0 lR,S) (211" 3~), 4.32--~.,06 (~, 2~) ~ 3.72 ld, J~ , 2~), 2 ~ ~2 (t , J~lOHz , 2B), 2 . 16~ 611), 1. 42-1. 08 (m, 14~1 ~nd 0 . 92~7 . 0 (m, 6~) C2~5 16 c~3lC~2)3c~c~2 (D~qSO-d6) 8.2-8.0 (S) ~ay 3~ . 2~-4 . 1 l~, ;~1~3[) t 3.7~ 3.60 5m, 21~), 2 . 18 ~t~
J~7, 2~), 2.02 ~d, J~7, 2H~, 2 . 02-1. 6 - (~, 3~1, 1.26 ~d, J~6 , 3EI), 1 . 26-~ . 08 5~Q" 8El) and . 92-. 74 (~, 6HI
, 3~ . .
.
- 35 - ~ 5~ 72222-8 N-(3-(S)-Methylheptanoyl)-D-gamma-glutamyl-L-alanyl-D-alanine (Rl = (5) CH3(CH~)3CH(CH3)CH2-;
R2 = CH3, R3 = ~NHCH(CEI3)CO2~) 12A. N-t-butoxycarbonyl-L-alanyl-D-alanine benzyl ester To a solution of 23.0 g. (0.121 m) of N-t-butoxycar-bonyl-L-alanine, 42.6 g (0.121 m of D-alanine benzyl ester ~-toluenesulfonic acid salt and 17 ml. (0.121 m) of triethylamine in 400 ml. of cold (0C.) methylene chloride was added dropwise 25.0 g. (0.121 m) of dicyclohexylcarbodiimide in 100 ml. of methylene chloride. After stirring overnight at room temperature the solids were filtered and the filtrate concentrated to an oil. The resi-due was dissolved in 400 ml. of ethyl acetate which was washed with a 1% hydrochloric acid solution, a 10% potassium carbonate solution, water and a brine solution. The organic phase was separated, dried over magnesium sulfate and concentrated to an oil. The residue was triturated with diethyl ether and the resul-ting solids filtered under nitrogen, 16.0 g. An additional 12.7 g. of the desired product crystallized from the filtrate.
12B. L-alanyl-D-alanine benzyl ester hydrochloride To a slurry of 28.7 g. of N-t-butoxycarbonyl-L-alanyl-D-alanine benæyl ester in 150 ml. of dioxane was added 150 ml. of dioxane saturated with hydrogen chloride and the mixture stirred for 4 hours at room temperature. The solven-t was removed ln vacuo and the residue triturated with diethyl ether. The resulting solids were filtered, redissolved in methylene chloride and the solution concentrated to about 150 ml. Ether was added and the solids filtered under nitrogen, 22.0 g.
12C. N-t-butoxycarbonyl-D-gamma-glutamyl (alpha benzyl ester)-L-alanyl-D-alanine benzyl ester To a slurry of 5.0 g. (9.64 mm) N-t-butoxycarbonyl-D-gamma-glutamine alpha benzyl ester dicyclohexylamine and 2.76 g. (9.64 mm) of L-alanyl-D-alanine benzyl ester hydrochloride in 100 ml. of methylene chloride cooled to 0C. was added 2.0 g. (9.64 mm) of dicyclohexylcarbodi-imide in 20 ml. of the same solvent. After stirring overnight at room temperature, the solids were filtered and the filtrate concentrated ln vacuo. The residue was treated with 150 ml. of ethyl acetate, the solids filtered and the filtrate washed with 1%
hydrochloric acid, a 10% potassium carbonate solution, water and a brine solution. The organic phase was dried over sodium sulfate and concentrated to give a white solid, which when triturated with ether and filtered gave 4.1 g. of the desired product.
12D. D-gamma-glutamyl (alpha benzyl ester)-L-alanyl-D-alanine benz~l ester hydrochloride To a slurry of 4.1 g. (7.21 mm) of N-t-butoxycarbonyl-D-gamma-glutamyl (alpha benzyl ester)-L-alanyl-D-alanine benzyl ester in 50 ml. of dioxane was added 100 ml. of dioxane saturated with hydrogen chloride, and the reaction mixture stirred for 3 hours at room temperature. The solvent was removed under vacuum and the residue triturated with diethyl ether, 3.5 g.
~r .:
12~. N-13 (S)-~ethylheptanoyli-D gamma-gluta~yl (alpha To 1.0 g. (1.98 ~ml o~ D-qamma-gluta~ lpha benzyl ester)-L-alanyl-D-alani~e benzyl ~ster and .833 ~1. ~5.93 mm) of triethyl amin~ in 50 ~1. of ~ethylene chloride was added 390 mg. (2.37 mm) of 3-(5)~methyl-heptanoyl chloride and the reaction ~ixture stirred under nitrogen for 45 ~inutes. ~he reaction w~s poured into 150 ml. o~ ethyl acetate ,and the organic phase wa~
washed with 10~ hydrochloric acid, ~ 10% potas~ um carbonate solution, water and a brine solution. The organic phase was dried over sodium ulfate and conce~-trated to dryness. The residue was triturated with ether and filtered under nitrog~n, 900 mg.
12F. N-~3-(S~-methylheptanoyl-D-gamma-glutamyl- .
L-alanyl-D-alanine A mi~ture of 200 mg. of palladium hydroxide on car~on and 900 mg. of N-~3-(S)~methyl~eptanoyl)-D-gamma- ¦
2D glutamyl ~alpha benzyl ester~-L-alanyl-D-alanine ben2yl ester in 50 ml. of methanol was sh~ke~ in a hydrogen I :
a~mosphere at 5~ psi for one hour, The catalyst was filtered and the solvent removed in vacuo. Water was added to the residue and removed under reduced-pre~sure to give ~92 mg. of the product as a white solid, m.p~ ¦
165-168C.
The N~R spectrum (DMSO-d6) showed a~sorption at 8.21-7.98 tm, 3~), 4~41-4~1 tm, 3~j, 2.3-2006 ~, 4~0 2.06-1.56 lm, 6~), 1.43-1.02 (m, llH) and 1.02 0.73 (m, 3~ 6~) ppm7 N-(3-(S,R)-Ethylhexanoyl)-~ ga~ma-glutamyl (alpha n-butyl estes)-glycyl D-alanine ( 1 C~3~C~z)2C~(c2~s)c~2~; R2 ~ H;
R3 - -N~ ~(CH3)CO~ C~
J -~ C~ y /
13A. N-t-butoxycarbonyl-D-gan~a-gl~t~Din~~~alpha A solution of 39.5 g. I0.,172 m) of N-t-bu~oxy-1~ carbonyl-D-glutamic anhydride in 75 ml. of dry tetra-hydrofùran was added dropwise o~verba~at~ol hour period to a solution of 4~ ml. (0.516 m~ and 34.3 ~1. (0.172 m) of dicyclohexylamine in 300 ml~ of ether at 0C. The reaction was allowed to ~tir at 0C. for 3 hours and ~as 15 . stored in a refriger~tor overnight. ~he solids were filtered, slurried in ethanol and filtered, 43.3 g~
13B. D-gamma-gluta~yl (alpha n bu~yl ester)-glycyl-~ h~ product of Example 13A tlO g.~ 0.021 ~ and 6.7g. (.024 ~) of glycyl-D-ala~ine ben~yl este~ hydro-chloride were slurri~d in 200 ml. of methylene chloride under nitrogen and cooled to 0C. Dicyclohexylcarbodi-imide (4.25 g., .021 m) was added and the ~ixture allowed to warm to room temperature overnight. The urea byproduct was ~ilte~ed and the solv~nt re~ov~ in vacuo.
The residue was treated with ethyl acetate and filtered~
The filtrate was washed succes~ively with water, 2.5%
hydrochloric acid, water, 10% potas6i~ carbonate and brine. The organic phase was dried over magnesium sulfate, the solvent removed under vacu~m and the ~,Z~ j7 '~3 L~
, residue dissolved in 300 ml. of dioxa~e ~aturated with hydrogen chloride. After stirrin~ 4 ho~rs at room temperature, the solvent ~as removed ana the residue triturated in ethyl ac~tate-h~xane (1:1) and filtered, 7.4 g.
13C. N-(3-(S,R)-ethylhexanoyl-D-glutamyl(alpha n-but~1 ester)qlycy~-D-alanine _ __ To the product of Example 13B (1.0 g., 2.35 mn) and .99 ml. (7.05 mm) o~ triethylamine in 50 mlO of I0 methylene chloride was added 460 mg. l2.83 mm) o~
3-(S,R) ethylhexanoyl chloride and the reaction ~tirred overnight under nitrogen. The solvent ~ai~ re~oved in vacuo and the residue dissolv~d in ethyl acetat~. The 16 organic phase was successively wash~d ~ith 10%
hydrochloric acid, water 10% potassium carbonat~ and .
brin~. The organic phase was separated, dried vver ~agnesium sulfate ~nd the solvent remoYed under vacuum. :
Th~ residue was dissolved in ~0 ml. of emtha~ol and shaken with 170 mg. of 10~ palladium hy~roxide in a hydrogen at~osphere at an initial pres~r~ o~ 50 psi for 1.5 hours.
The spent ~atalyi~t wa~ filtered and th~ solvent removed in vacuo, 100 mg.
NMR ~D~SO-d6): 8.18 (d, J - 6, 1~ o 8.10 (d, J=6, 1~), 8.02 (t, J-5, 1~), 4.28-4.10 (m, 2~ .09 it, J-6, 2H), 3.78 3.56 Im, 2~), 2.18 St, J 6, 2~), 2002 (d, J=6, 2~), 2.00-1.60 Im, 3H), 1.58-1042 Im, 2~), 1.28-1.08 (m, 8~, 1.24 ld, J=6, 3~)~ 0.92-0.76 Im, 9~).
: 30 ~S~
EX~MPLE 1 4 Employing the general procedure of Example 13, and starting ~tih the requisite reagen~s, the ollowing s:ompounds were prepared:
O
RlCN~ ~ I) ~ C2R4 fH D
C O ~CH2) 2CONE~CE~2CON~CEI(C~3~2 ~ R4 D~R
3 -methylheptanoyl methyl Nlq~ (DMSO-d6 ): 8 . 23 ~d , J~ , 1}1), 8 . 15 (d, J -6 , 1}1), 8 . 03 (t , J~6 , 11~), 4 . 28-4 . 1~ (m, 2E~); 3 . 72 ~d, J-6, 2E~), 3.61 (s, 3~ 3 ~t, J-7 , 2~), ~.16-1.70 Im, 6EI), 1.34 loû4 (~a, 8~1) t 1.25 ld, J~75 31~) 9 o.s2;-o.?~ Sm, ~:
6~).
.
. .
-.~2~7~
.J~ ~/ R,a N~fn 3-ethylheptanoyl methyl NMR (Dl~SO-d6~: 8.19 (d, J~6 , 1~), 8 . 1 1 ~d , J~6 , 1~, 8003 ~, J-6, 1~
4 . 2~-4 . 10 ~m, 2~), 3 . 74- : ' 3,62 ~, 2~), 3.57 I~.
3~, 2.18 (t, J-9, 2H), 2.01 ld, Js6~ 2~1, 1.97~
1 . 60 ~m, 3~), 1 . 32-1 . 10 ~m, 88), 1 . 23 (d, J-7 , ;
3EI), 0.90-~.72 (~, 6~) :
ll . 3-methylheptanoyl ethyl N~R ID~SO-d6) s 8.. 248.n2 (m, 3~ .26-3.56 (m, ~ ~ .
~, 4.04 (q, J'9 3.76-3,56 ~m, 2~) ~ 2~17 ;
(t, J~7, 2~ . 12-1 . 63 (m, 6~); 1 . 74-0 . 9d, ~m, 6~), 1.23 (d, J~5, 3~), 1.13 ~:, J~9, 3~;, 0.8~-0 . 72 (m, 6~3 ~ ;.
' 5~
, '~ R, c o .~ 4 ~R
3-ethylhepta~oyl ethyl NMR (D~SO-d6): 8.16 (d, J#6, 1~), 8.09 ld~ J~6, ~ 8~02 ~t, J~6, 1~), 4.~2-4.08 lm, 2~), 4.D2 (q~ J~7, 2~), 3.7~-3,54 ~m, 2~3, 2.1~ (t, J-7, ~:~
2~1, 2,00 (d, J~6, 2~j, 1.96-1O58 (m, 3~), 1.30-1.08 (~, 3~), 1.21 (d, ~'7~ 3~) t 1.13 (t, J~7, 3~)~ 0O88 0~70 (m, 6~) 3-methylheptanoyl iso- NMR tDMSO-d6): 8.14 (d, bu~yl J~6, 1~), 8.08-7.98 lm, 2~), 4.20-4.04 (m, 2H)~
3.75 (d, JY6~ ~), 306B- :
3.54 (m, 2~), 2.14 ~, J~6, 2~ .08~ t 1.28-0.96 ~, 6~), 1~19 Id, J~7, 3~, 0.88 0.70 ~m, 12~) as 1":, .
~ ' '.
:
: ' ' '',.
'' .'' , .'~
~ ~ R ~
__ .
3-ethylheptanoyl iso- ~MR tDt~lso; dc): 8 .21 (d, butyl J~6~ 1~) t 8.1~-8.04 (m, 2B), ~ . 24-q . 08 ~, 2El), 3-79 Id, J-6, 2E~1 I 3.72-3.58 (m, 2~1), 2.19 (t,.
J~7, 2B), 2.03 5d, J-6, 28), 1 . ~9-1 . 6~ (~, 4~
1 . 32-1 . 10 ~, 81~), 1 . 23 (d, J-6, 3EI), û.g~ 0~72 Im. 12~) 16 3-ethylh~xanoyl isc>-- NMR (DNSO~-d6). 8.18 (d, butyl J=6 , lH~, 8 . 10-8 . 00 (m, 2~) ~ 4.26-~.08 Ir~, 2~I), 3.79 (d, J~6 , 2~33, 3~,72-3.58 Im, 2H1, 2.18 tt, J~6~ 2~), 2.02 ld, J~6, 2B), 1.98-1 . 62 ~m, 4E3 ), 1 . 3q-1. 08 (mO 6EI), 1 . ~3 ~,d, J~7; 3~), 0.96-0.72 (m, 12 ,,~.
, :, '' . .
, .
i7~3~
~ i -44~
-''' R,~-C
3-ethylhexanoylmethyl ~R (D~IS0~63: 8.21 ~d, J~7, 1E13, 8.10 (d, J~7, 11~), 8 . 05 (t, J~6 , lH~, 4.,26-~ .10 ~m, 2H), 3.76 3.~0 (~, 2EI~, 3.5g (~, 3~), 2.18 (11:, J~6, 2~
2.02 (d, 3~6, 2H), 2~0û
1 . 60 (~, 311), 1. 3~ 1 . 08 (~, 7EI), 9.90-~.72 (~
611) .
3-ethylhexanoyl ethyl pa~qR (D~S0~6) 5 8.22 (d) J~7, 11~ " 8.l8-a.~6 (m, ~3, 4 . 26-4 . 10 (m, 2~), 4,.06 Sq" Js5, 2Hl, 3.7~
3 0 58 ~m, 2E11~ 2 . 20 (~ , J~6 , 21~1, 2 . û4 Id , J-6 , 21I~, 2.02-1.60 Im,, 3H) 0 1~26-~.20 (~3, 7~I~, 1.18 t , J~5 , 3~1~, 0 . 90-0 0 7 ~o ~13 .
:io `
57~3~
~!
--~5--O
R4 ~R
__ __ 3-~ethylheptanoyl butylNMR ~DI~ d6): 8 . 20 (a, J~7, lE~ ~ 8.16 B.04 i;o, 2~1), 4 . 24-4 ~, 0S ~m, 2EI), 4.00 (t, J~6, 2H), 3.7~-3 . 56 ~m, 2EI~, 2 . 17 ~t, J~6 , 2}1), 2 . 12~ 0 ~, !
5}~ 1.58-1.40 ~m, 2~
1.36-l.00 ~, 8~), 1.21 (d~ J-6, 3~), 0.90-0.7~ :
(m, 9EI) 3-ethylhep~a~oyl butyl N~R ~I)IqSO d6): 8.16 ~d, J~7, lEI) " 8.11 (d, J57, 1~, 8.03 ~, J~5, 1~, 4.26-4.09 ~m, 20~1), 3.99 (t , ;1~7 , 2~I~, 3 . 79-3 . 58 (m, 2H), 2.17 (t, 3~6y 2}1~ 2.01 ~a, Js6, 2~), 2.00-1,,60 ~m~ 3E[) ~ 1058-1.42 I~, 2~11, 1.36-1.08 (~, lOEI) ~ 1.24 (d, J-S, . :
3~, 0.92-0,,72 (~, 9El) !
.
,:
.
.
~ 7~3~
~6-__ N-~3-(R,S)-Ethylhexanoyl~-D-gamma-yl~tamyl glycyl-D-alanine ethyl ester ~Rl ~ C~3(C~212C~C2H5)C~2 ;
R ~ ~; R - -~NC~C~I3)C02C~
S 2 3 ~ 5 15A D-gamma-glutamyl (alpha benzyl ester)glycyl-D-alanine ethyl ester h~d~ ~
To a ~lurry of 14.8 y. ~.0285 ~) of N-t-butoxy- :
carbonyl-D-gamma-glutamic acicgl alpha benzyl e~ter dicyclohexylami~e salt acid ~ . V285 ~) o glycyl-D-alanine ethyl ester hydrochloride in 200 ml~ o methylene chloride was added 5.6 g. ~0270 m.~ o~
dicyclohexylcarbodiLmide an~ the mixt~re ntirred under a nitrogen at~osphere overnight. The ~rea i~ filtere~ and the solvent removed in acuo. The r~sidue was treated with 300 ml. of ethyl acetate, filtered ~nd the filtrate washed successively with 2.5% hydrochloric acid, water, 10% potassium carbonate solution and brlne. The organic phase was separated, dried over magnesium and concen-trated under vacuum. ~he residual oil ~as dissolved in 450 ml. of dioxane saturated with hydrogen ~hloride.
The ~olution was stirred ~or 2 hours and the solvent removed in vacuo. The resi~ue was triturated with ether :
an~ filtered, 11,2 g.
15~. ~(3-~R,S)-e~hylhexanoyl)-D-gam~a-glutamyl- :.
~lvc~l-D-alanine ethYl est~r :
. , ~ _ _ , . . .
To the product o Exa~ple l5A 11.0 g., 2.33 ~
and .98 ~ 6..98 mm.,) of triethylam~e in 30 ml, of methylene chloride, under a nitrogen atmosphere, was added 378 mg. ~2.33 ~.. 3 of 3-(R,S)-ethylhexanoyl chloride. Aft~r stirring at room te ~ :rature for 1.5 hours the mixture was poured into 100 ~1. of ethyl .... . . ..
_ 47 _ ~ ~ S 7~ ~2222-8 acetate and the organic phase was washed successively with 10%
potassium carbonate solution and brine. The organic phase was separated, dried over magnesium sulfate and concentrated in vacuo.
The white solid residue was dissolved in 30 ml. of methanol and hydrogenated over 0.1 g. of palladium hydroxide in a hydrogen atmosphere at an initial pressure of 50 psi. After 2 hours the catalyst was filtered, the filtrate concen-trated to dryness and the residue triturated with ether and filtered, 275 mg.
NMR (DMSO-d6) 8.26 (d, J=9, lH), 8.14-8.02 (m, 2H), 4.31-4.00 (m, 2H), 4.06 (q, J=10, 2H), 3.78-3.60 (m, 2E), 2.17 (t, J=8, 2H), 2.08-1.65 (m, lH), 2.03 (d, J=8, 2H), 1.82-1.53 (m, 3H), 1.40-0.96 (m, 5H), 1.23 (d, J=6, 3H), 1.14 (t, J=10, 3H), 0.90-0.64 (m, 6H).
Starting with the appropriate reagents and employing the procedure of Example 15A-15B, the following compounds were pre-pared:
O
RlCNH \ D / C02H
CH D
(CH2)2CONHCH2CONHCH(CH3)CO2Rs X
~2~ 34 --48-- ;
,O
,~, , , 3R5 ," ,NM ... t. "
3-methylheptanoyl iso-butyl MI~R (Dt4SO-d6): 8.24 ~d, J~6 , 1~), 8 . 1û-8 . 00 ~m, 21~ . 30 ~ .18 (m, . O~ ~m, 1 3 . 86-3 . 72 (~n, 2H~, 3.72- 3.58 ~m,, 2~), Z.16 ~t, J-6 , 2El), 2.12--1~64 ~, 6~I1 D
1.52-1. 00 Im. 6~
1 . 27 ~d o J~7 ~, 3El), 0.90-0.76 (m, 12~) 3-ethylhexanoyl iso-butyl ~R ~I:)MSO-d6): 8 . 23 (d, J~6, lEI) " ~.0~7.99 ~mg 21~), 4.29-4~17 ~m, lEI), :
4 . 17-~. 07 (~, 1~), 3.83-3.71 ~m, 2~), 3.71-3~5$ (m, 2El) ~ 2.15 .
(t, J~7~ ~), 2.0~-1.60 ~gl, 4~), 2.00 ~d, J~6, 2~3, 1.31-l~Og Im, 6~1), 1 . 25 5d, J~6 , 3~), 0.90-0.72 (m, 12~I~
-.2~3~7~3~g, ;
-49- .:
0 R5 N~
3 ethylheptanoyl iso-butyl NMR ~D~SO~d6): 8.23 (d, ;
~8~ lM), 3.08-7.98 ~m, 2~) ~ 4~29-~ol8 tm, 1 4.18-4.07 (m, 1~), 3.86 3.72 (m, 2~, 3.70-3.57 tm, 2~, 2.15 (~ J-7, 2~), 2.04-1.5g t~, 4~), 2.00 (d, J~6, 2~), 1.30-1.11 (m, 8~), 1~25 ~d, J36, 3H), ~.89l0.70 (~ 9 12H) lS c~c/oh~ ne7~
3-methylheptanoyl ~ }- NM~ ~D~SO-d~): 8.25 (d, ~ye~he~t~ J~6~ 1~), 8.13-8.00 ~m, 28~, 4,32-4.20 (m, 1 4~20-4.08 ~m, lH3, 3.91-3.76 (~, 2~), . 76-3.5~ (m, 2~), 2.1 ~tJ J~ ), 2.13-1.48 .
(m, 8~), 1.36-1.01 Im, 12~), 1.27 ~d~ J56, 3~), 1.0~ 0.76 t~
50- .
R~ CO ,,~
3 ~}~ ~5 ~_ , . .
~)~Clo~< y/~n ~f~y/
3-ethylhexanoyl -a~L- NMR ~D~SO-d6~: 8.~5 ~d, _4~4~L~h~ J~6, 1~), 8.13-8.09 (~, 2H), 4~32-4~20 (ffl, 4.20-4.08 ~m, 1 3.9~-3.7~ (~, 2~
3.74-3.59 ~m, 2~), 2.18 tt, J~6, 2H3, 2.09-1.86 (m, 1~), 2.03 (d, J~6, 2~), 1.82-1.43 ~m, 8~
1.36-I~01 (~, 9~), 1.27 (d, ~6, 3H), l~01-0.70 l6 (m, 8 ~yc/~e~ y/h~ y/
3-ethylheptanoyl -met~y~ NMR tD~SO-d~): 8.26 ~d, h~hexrl- J-6, lR), 8.12~8.02 ~m, 2~ .31-4.1~ (m, 1~), 4.19-4.0~ ~m~ lH), 3.93-3.7~ (~, 2~), 3~72-3~58 (~, 2~, 2.18 ( , J~6, 2H), 2.08~1.86 (m, lH), 2.03 2S (d, J~6~ 2~)o 1.82-1.48 (m, 8~), 1.34 1.02 ~m, 11~), 1.27 6~, J36, 3~ 00 0~7~ lm, 8~ :
:
. .
;i7~
(` 0 ~ ~, R5 ~MR
.; . , .-3-methylheptanoyl ethyl N~R (D~S0-d6): 8.25 (d, J~6, 1~), 8.12-B.00 (m, 2~), 4.28-3.96 ~m, 2H)~
4.03 ~q, J~7, 2R), 3.74-3~56 ~m, 2~), 2.16 (~, J~9, 2~), 2.11-1.62 0 Sa9 6~), 1.32-0.98 ~m, 6EI), 1 . 24 ~d , J~7 , 3H), 1.14 ~t, J-7, 3H), 0.88-0~76 ~, 6~) 3-ethylheptanoyl e~hyl ~MR ~D~S0-d6~: 8.28 ~d, J~6; l~l, 8016-8.04 ~m, 2~), 4.32-4.04 ~m, 2H)~
4.10 (q, J36 9 2~, 3.~8-3.6~ ~, 2~), 2.22 (t, J-6, 2~), 2.11~1.92 ~ 3, 2.07 (d9 J 6, 2~ 1.8601064 S~, 2~
1.~0~ m, 8~), 1030 (d, J~69 3~), 1.21 (t, ~J~6, 3~), 0.94~0.7 2~ (~, 6~
i7~
.
R/_~D
R5 ~MR.
-- ;
3-ethylhep~ar~oyl l~utyl N~IR tD~IlSO~d6): 8.27 Id, J~8 , 1~1~, 8 ~ . 02 (al, :
21~ ,.32~4.1û (m, 2El), ~l i lO-3 . g~ ~, 2}I), 3.7a 3.60 ~, 2~I), 2.18 It, 3~6 , 2~, 2 . 0~a ~d, J-~ , 2E~, 2 0 0~-1 . 62 3~), 1060-1.46 Im, 2 1.38-1 . 10 I~, 12~
1 . 27 ~d , ~-6 , 3}I), 0~,90 0.75 Im, 9~1) 3-S-~et~yll~eptanoyl butyl W~ (DMSO-d6): 8.30 (d, J~8, 111), 8.15-8.04 ~, 2Ba, ~.~S-~.12 ~, 2~), !
~L r l 2 3 ~ 9 ~
3078-3~65 (~ 2~ 2~22 (tJ J~7~ 2EI) ~ 2~18-1~69 .-~" 7EI), l.G1~1.48 (~, 21~), 1.~0-1.11 (m, llB), 0 . 97-O . B0 (m, 9 30 - . ., ~5i7~3~
, ` . ~ ,.................................................... ..
~ 5 NMR
3-S-ethylheptanoyl butyl N~ ID~SO d6): 8.22 (d, J87, ~, 8.12~.0 ~, , 2H), ~ 6 (m, 2~, 4.08-3.95 ~m, 2 3.75~3.~2 t~
~t, J~6, 2~), 2.02 ~d, J~6~ 2~), 2.04-1.6 3~), 1.6~-1.46 I~, 2~
1.38-1~1 1~, lS~), and .9_.75 ~m~
. :
lS 3-ethylhexanoyl butyl NMR tDMSO-d6): 8~28 (d, J~8, 1~), 8.14-8.04 Im, 2~ .34~4.10 (m, 2H), 4.10-3.95 (~, 2~
3.75-3.62 Im, 2~), 2.19 2~ (t, J~6, 2~), 2.04 (d, J~6, Z~), 2.04-1.6~
(~, 3~), 1.60-1.45 Im, . .
2~), 1.40-1.10 (m~ :;
13~), 0.90-0.76 (m, g~) ;1 2~ 1 '~,.
i ~
--5~
EXAI~PLL 1 7 The procedure of Example 15 i~ a~ai~ ~epeated, starting with the appropriate reagents, ~ith the exception that the hydrogenation i8 not casried out, to give the follo~in~ compou~ds:
Il . 1' RlCM~ ~D ~ C02R4 ;:
C~[ D
'1~23 2CONHC:E~2CON;E~ C~3~c2R5 ,;
O ,:
~;;` ~ R R5 NMR
3-ethyl- butylbutyl N~R (DMSO-d6): 8.27 (d, J~7, hexanoyl 1~), 8.20 ld, ~7, 1~), 8.07 (t, J~70 1~ .37-4.13 (~, 2~3, 4.0~ (t, J~6, 4~), .
3.80-3.62 Im, 2~), 2.20 ::~
~t, J~6, 2~, 2.n5 (d, J-6, 2~), 2.92-1064 (m, 3~1, 1.60- :
1.47 (m, 4~3, 1.40-1.13 (m, ..
13~), 0~95-0O~7 (m, 12~3 3-ethyl- butyl butyl NMR ~DMSO~d63: 8.27 ~d, J-7, heptanoyl lH); 8.20 ~d, JD7Y 1~3 ~ 8-06 (t, J~6, 1~, 4.36-4.13 (m, 2~), 4.02 (~, J-~, 4~), 3.80-3.6q (m~ 2~), 2.20 - l~, ~=6, 2~, 2.04 (~, J=6, .
2H~, 2.00-1.60 g~, 3~) t 1O60-1.~3 (m, 4~3, 1.40-1.10 ~, ~ t 0.95-0~72 (~, 12H3 -3571~
,~$~ o _55_ .
~1 R4 R5 N~gR
3-methyl butyl butyl N~IR (DMSO-d6~: 8 . 26 (d, J~7 , hepta~oyl 1~ . 19 (d, J~7 , lEI), 8 . 07 ~t, J~6; 1~), 4.32-~.11 lm, 2~ . 02 5~ 0 J~5 t 4EI), 3 . 79-3 . 59 ~m, 2R) O 2 . 20 (t, J-6, 2~) f 2.14-1.68 Im, 5H7, 1 . 61~ .6 (m, 4H~, 1 . 40 -1 . 06 Im, 13~), 0 . 95-0 . 81 (m, 12~I) 3-S benzyl benzyl NMR (DMS0-d6): 8 . 33 td, J~7 , 15 methyl~ , 8 . 24 ~d, J~7 , 1~), 8 . 08 heptanoyl (t, J-5, 1~), 7~33 (s, 10~
5.08 ~s, 4H), 4.40~4022 (mt ~}~), 3.~û-3.60 ~ , 2.2 (~, J-5~ 2~), 2.14-1.64 ~m, 20 5~I), 1 . 26 ~ 7, 3~), ;
1.22-0,98 ~m, 6El), 0.88-0.73 (~, 6~1 ..
;
:j, ~,,, , ':
2~ ~7 ~ ~ ~
EX~MPLE 18 Crystalline ~-(3-tS)-methylhep~anoyl)-D-gamma-qlutamyl-qlycyl-D-alanine N-(3-~S)-Methylheptanoyl)~D-qamma glutamyl (~lpha benzyl ester)-glycyl-D-alanine benzyl ~ster (30.8 g.) was slurried in 300 ml. absolut;e eth~nol ~n a 2 liter autoclave. 5~ Pd/C, 1.54 g., S0~ water wet) was added and ~he ~ixture hydrogenated at: 4 x atmosph~ric pressure for 1 hour, by which time uptake of hydrogen was I complete. The catalyst was rec:o~ered by filtratio~, fir8t over paper, then over 0.~5 ~icro nylon ~ilipo~e, employing 100-150 ml. ethanol or tran~fer a~d wa6h.
The com~ined filtrate and wash liquors we~e stripped to 1~ a damp, white solid, which was di~solved ~n a 150 ml.of a hot, 1:10 mixture of ab~olute ethanol and acetonitrile, clarified by hot filtration, boiled down to 35 ml., 810wly cooled to ro~m temperature, granulated and fil~ered to yield crystallin~, den~e, non-electro-static tit~le product, 20.1 g. (94~) characterized by it8 ir (~u~o~ mull) ~hich includes major, well-r~olved, sharp peaks at 3340, 3300, 2900, 2836, 1725, 1650, 1628, 15B0, 1532, 1455, 1~10, 1370, 1280, 1240, 1276 and 1175 Cm~l This crystalline product (9.4 g) was further purified by dissolving in 1000 ml. of acetone at reflux for 1 hour. The solution was cooled to room temperature and seeded with a trace of the above cryctals. Afte~
stirri~g for S hours, title pro~uct was recovered by filtration with minLmal acetone wash, and dried ln vacuo at 35C., 7.25 ~., having identical ir characteri~tics.
p~
, 7~
-~7- :
N-(3~ Methyl-~-heptenoyl~-D-g~mma-glutamyl (al~ha benzyl ester~-~lyc~l-D-alani~e be~ l ester Following the procedure o:E ~xample lOD, 2.77 g. (5 mm) of D-gamma-glutamyl ~alpha benzyl e~ter) ;~lycyl~
D alanine benzyl ester hydrochloride an~ the acid chl~ride prepared ~ro~ 747 mg. (5 mm) o~ 3-(Rj-methyl-4-heptenoic acid gave the titled co~pound.
XA~PLE 20 N-(3-(S)-~ethyl-4-heptanoyl)-D-gamma~glutamyl-cllYc~l-D-al.mine A mixture o~ 500 ~g. of the product from Example 19 and 26 mg. of 5~ palladium-on-charcoal ~50~ water wet) in 125 ~1. of ethanol was shaken in a hydrog~n atmosphere at an initial pressure o~ 4 x atmospheric pressure for 2.5 hours. The cataly~t was iltered and the solvent removed i~ vacuo. The produ~t was purified by the procedure of ~xample 18, and was identical in all 2~ respects to the prod~ct of that exa~ple.
, .:
2~ ~''' ,::
. ~ ~ .. ~ .. . . . . . . . .
-:
i7~
:' PREPARATIOR A
Al. ethyl cvcl~ e-e To 4.9 g. of 60~ sodium hydride in oil wa~ ~dded sufficient hexane to di~solv~! the oil. To the oil free sodium hydride under nitrogerl ~as added 100 ml. of dry tetrahydrofuran followed by ~l solution of 22.2 ml. of triethyl phosphonoace ate in 80 ml. of dry tetrahydroD
furan. After stirring at room te~perature ~or one hour 10.5 ml. of cyclohexanone wa~ added i~l ~0 ml~ o~ tetra-hydro~uran and the reaction ~ixtur~ ~tirr~d at room temperature overnight~ The reaction ~ pourad into water and extracted with diethyl ether. ~he or~anic lS phase ~as wa~hed with lN sodium hydroxide ~oluti~n, water and brine. The organic phase was sep~ra ed, dried over ~agnesium sulfate and concentrated under reduce~
pre~sure .
~he residue was dissolved in 250 ~1. of ~ethanol, 23 trea~ed with l.S g. of 10~ palladiu~ hydroxide on carbo~ :
and the mixture ~haken in a hy~rogen atmo~phsre at 5 0;i psi for ~ hours. The ¢atalyst was filtered a~d the f iltrate conce~ltrated in vacuo . The residue wa~ dis-tilled at 45-50C. /0 . ~ torr to give 15 . 4 g. (9096 yield) of the desired intermediateq ~::
"
A2. cyclohexylacetyl chloride To 100 ml. of metha~ol ~s~taining lS.~ g. of ethyl cyclohexylacetate was added 15.2 g. of pot~ssium hydroxide and the 301ution refluxed for 3 hours. The ::~
methanol was removed ~n vacuo and the residue trea~ed with ~ater. The ~olution ~as extracted with diethyl ether and then a~idified with 10~ hydrochloric a~id~ ;
The acidified ~olution wa~ e~tracted ~ith fresh ether and ~he organic phase ~eparated and ~ashed with ~ater . . . , - :
3~ii7~
59 ~:
and a brine solution. Removal o~ the ~olvent after drying gave a liquid residue.
The residue was dissolved in 60 ~l. o~ ~ethylene chloride was treated with 18 ml. o~ oxalyl chloride.
After ~tirring at room temperature ~or 4 ~our~ the reac~ion mixture was concentrated und~r vacuum and the residue distilled~ 45/50C.t0.4 torr, 12.5 g~ ~86 yield).
PREPARATION B
Following the general prooedure of Pr~paration A
and starting with triethyl phosphonoacetal ~nd the appropriate aldehyde or ketone the following ~cid chlorides w~re prepared~
IS ~lCO~l l Z.P.-C./torr ~C~13C~2C~2i 2CEICEI2 ..
tC~3CH2)2C~c~2 22-~5/0.5 :
1~3 C~3(C~2)3C~C ~ 23-30~0.5 C~ -C~3~C~2)2~C~ - 22-25J0 ~R,S~
3a (c~3)2c~(c~2)3 Z4-3110.7 '~.' . .
,~ . . . ~
.; .
i7~3~
Rl ~ L~ ;
f~3C~2 .,, C~13 (~ ~2 ) 3CHCH~ 3~-37/ 0 ~ 5 ' f~3 :
CH3 (CH2) 4C9CH 45~47/O.li ,S~ :
25-30/O.S
~CH3C~2) 2CE~(C~2) 2 32 36/0.~ ~
Cl~ I .:
1 3 ,:
CH3 (C~2~ ;! tHR(S~ 2) 2 30-3~/.06 :.
I 3CH2 .'.
C~3 (C~2) 4 (RCS~ 63-65/ .35 CH3 (C92) 5 ~RCS j~ 89~215 :' f~3 (C~332C~(C~2),~C~C~2 46~;50/0~5 7~
-61~
Rl ~
CH ' "
1C~3)2C~C~2C~C~ - 30-34~.5 :
~R,S~ ~
., 3IH2 ..
(R,S~ 31-35/0.7 :;'i PREP~RATION ~
5~11~= .
Cl. 3-hydroxy-4-meth~ pentene To 90 ml. of l.OM vinyl magnesium bro~ide in tetrahydrofuran cooled to ~C. was added dropwise 6.3 -:
ml. of i~ob~tyraldehyde in 30 ml. of tetrahydrofuran and the mixture then allo~ed to warm to room temp~ratuse.
After 2 hours the reaction was added ~o a ~aturated ammonium ~hloride solution and extracted with ether.
The ether extracts were combined, ~ashed ~ith a .
sa~urated ammo~ium chloride solution~ a saturated sodium bicarbonate solution and a brine solution, and dried over magnesium sulfate. The solvent was removsd i~
vacuo to giv~ 6.0 g. of the desired product.
;.
:
' ' ',.
i~ ~ h e p ~eh o ~
'3~ C2. 5-~ethyl-4-he~n~e_acid ethyl ester A mixture of 18.2 g. of 3-h~dro ~ 4- ~thyl~l-pentene, 200 ml. of triethyl ~.r~e~EE~ and 500 ~1. of ~-toluenesulfonic acid ~as tre,ated with 400 ~l. oP
toluene and heated to reflux over 4A ~olecular ~ieve~
for 24 hours. The solvent was removed in cuo a~d the residue distilled~ The fractilon di tilling at ~5-64C./ ,~
0.5 torr ga~e 7OS g. of the desired pro~uct.
C3. 6-methylheptanoic acid ethyl ester To 7.5 g. of 6-~ethyl-4- ~ cld ethyl ester in 75 ml. of methanol w~s adde~ 700 ~g. of 10~ palladium hydroxide on carbon and the mixture shak~n in a hydrogen atmosphere at S0 psi for 1.5 hours. The cataly~t ~as filtered and the solvent removed under vacuum to glve 5.7 g. of the desired productO
C4. 6-meth~lhe~tanoyl chloride ~ ollowing the procedure of P~epa~atio~ A2, 5~7 g.
of 6-methylheptanoic acid ethyl ester gav~ 2~0 g. of the desired product, b.p. 30~34C./0.5 torr.
:
' ,',~.
I;
7~
PP P~TI~ D
~ y~oyl chloride Dl. 2-methylheetanoic acid To a cold (0C~l ~olution of 100 ~1~ o~ dry S tetrahydrofuran containing 11.8 ~1. o~ dry dii~opropyl a~ine and 55 ~1. of 1.6~ n butyl lithi~m was added 5.4 ml. of n~heptanoic acid and the mixture allow to stir at room temperature for one hour. The resulting solution was cooled to O~C. and 7.2 ml. of methyl iodide was added. The reaction was stirred at roo~ temperature under nitrogen for 105 hour~ 9 and wa~ then poured into 10% hydrochloric acid and extracted with diethyl ether (3 x 100 ml.). The extracts were co~bined, wa~hed with 10~ hydrochloric acid, water, 20~ ~odium bi~ulfite and a brine solution and dried over msgne~ium ~ul~ate. The solvent was removed in acuo a~d the residue, 5.61 g..
dissolved in ~ethanol containing 5.1- g. of potassium hydroxide~ After stirri~g overnight the mehtanol was ~0 removed and tbe residue dissolv~d in 150 ml. of water.
The aqueous layer was wa~ed with eth~r (2 x 100 ml~3 and acidified ~ith 10~ hydrochloric acid. ~he proauc was extracted with ether, washe~ with a 20~ sodium bisulfite solution and brine and dried over magnesium 23 sulf~te. Removal of th~ ether ga~e 5.0 g. of the product as a y~llow liquid.
D2. 2~ hvlhe~e-~ovl ~hio~id~
~ mploying 5 g. of 2-methylbeptanoic acid and 7 . 6 ml. of oxalyl chloride and usi~g the procedure of Preparation A2, 3.3 g. of the d~ired product was obtained, b.p. 32-34 ~C./0.6 to~xO
'7~
PREPARATION E
3-tS?~ L!~oe~lee~ a ~e`l ~9~b~olE~z~ ~on~ e ~VI ~e5f ,~ ~ re r To a 5 lo four ~eck~d fla~sk ~itted with a~ r~
and p~ electrode wa6 added 2.5 1. og ~01~ pota~ium acid phosphate buffer p~ 7.0 followed by 150 mg. of pig liver esterase and 150 9. of dimethyl 3-methylgluta~ate. The p~ of the mixture ~as maintaineaa at about 6.85 by periodic addition o a 10~ potassium carbonate ~olution.
After 2.5 hours the reaction was acidified ~ith 10%
hydr~chloxic acid to p~ 2.0 and the product e~tracted with diethyl ether. The extracts were so~bi~ed, dried over magnesium sulfate and concentrated in vacuo to give 1~ 114 g. of the desired product, lalphalD ~ -1.48 ~C~30 C=0.086 g/ml)-E2. met~l 3-(R)-m~Y¦C~ bo~
To 114 g. of 3-~R)-~ethylgl~taric acid ~ono methyl ester in 715 ml. of dry tetrahydrofuran cooled to 0CO
~as added slowly 391 ml~ of 2M solution sf bor~ne dimethylsulfide in tetrahydrofuran. After the ~ddition was complete the reaction mixture wa~ ~ti~r~d o~ernigh~
at room temperature. The reaction wa~ cooled and 50 ml.
of water slowly added. The reaction was extracted ~3 x 100 ml.~ with ekher and the extracts co~bined, washed with water, ~ ~aturated sodium bica~bo~ate 801ution and a brine solution, and dried over magnesium ~ulfate.
~emoval of the solvent gave 37 g. of the de~ired product. l, 3~ :
~ ,'~ 7~ ~ :
:, E3. methyl 3-~R~-methyl-5-(t-butyldimethyl-To a solution of 37 q. ~.253 ~ o~ ~ethyl 3-(R)-~ethyl-5-hydroxyp~nta~o~te and 37 g. l.5~3 ~) Of ~idazole in 500 ~1. of dLmethylformamide was added 37 g. ~.249 m) of t-butyldimethyl~ilyl chloride a~d the reaction stirred at room temperature for 2 hour~. The .:
reaction mixture was poured i.nto water ~nd ~xtracted ~4 x 10~ ml.) ~ith ether. The co~bined extra~ts were 10 washed with 10% hydrochloric a~id, a saturated sodiu~ ;
bicarbonate 801ution, water ~nd a bri~e solutisn, and dried over magne~ium sulfate. Re~oval of the solvent gave 12I.88 g. of crude product which, on distillation, gave 107.12 g. of pure product, b.p. 80 81C./0.4 torr.
E4. 3-(S)-methyl-5-(t-butvldimethyl~ilyloX~ Pentanol To 8.5 g. (.224 m) of lithiu~ aluminum hydride in 250 ml. of die~hyl ether under nit~oge~ was add~d 53.5 g. (.206 m) o methyl 3- ~R~ -methyl-5- (t-butyl~methyl- :
silyloxy)pen~anoate in 1~5 ml. o~ etherO The reactio~
was stirred for one hour at 0C. and wa~ then treated dropwis~ with 8.4 g. of water, 8.~ ml. of a 15% 80dium hydroxida solu~ion and 25.2 ml. of water. The solids were filtered and -he organic phase separated and washed 2S with water, 2.59~i hydrochloric acid arld a l:rine ~olution.
Th2 organic phase ~as dried over ~a~nesi~m ~ulfate and concentrated in vacuo to give 46 q. oiE produ~:t.
~:5. =~_~
~o 56.3 g. of oxalyl chloride in 3D0 ml. of dry methylene chloride cooled to -60C. 2nd under a ~i~r~gen atmosphere was added dropwi~e 74~81 y. of d~methyl~
sulfoxide in 100 ml~ o~ dry ~ethylene chlorideO After 15 minutes 92.0 g. oP 3-(S)-methyl 5 (t-butyldimethyl-silyloxy)-1-penta~ol in 250 ~1. of th~ same 301~e~t was i I., .
.:
-66- :
added dropwise. After 30 minutes 206.1 g. of triethyl-amine was added to -60C. followed by the r~moval of the cooling ~ath. The reaction was stirred at room t~mpera- :
ture for l.S hours, and was then poured into water a~d extracted with methylene chloride. The extract~ were washed with 2.5~ hydrochlori~, a 8aturat~d ~oaium bicarbonate solution, water and a b~ine 801ution, dnd then dxied over magnesium sulfate. The ~olve~t wa~
removed and the re idue dissol~ed in ether and rewashed and dried as before. Removal o~ the ether ga~e 90.9 g.
of the desired p~oduct.
E6. 5-(S)-methyl-7-(t-butyldimethylsilyloxy?-2-heptene To a slurry of 80 g. (.2155 m) of triphenyl-ethyl phosphonium ~romide in 800 ml. of dry tetrahydrofuran cooled to 0C. was added 165.7 ml. of 1,3~ ~olution of n-butyl lithium (.215S ~) in the ~ame ~olvent. After 2 hours, 45 gO ( r 196 m) 3-~R)-methyl-5-(t-butyldimethyl-silyloxy)-1-pentanal in 200 ml. of d~y t~trahydrofuran was added dropwise to the rea~tion mi~ture. The reaction was allowed to stir 2 hours at room temp~ratu~e and was then poured i~to wa~er and e~tracted with ether.
The combined extracts were washed with water and a brine solution and dried over magnesium ~ulfate. Removal of the solvent in vacuo gave a ye}low oil which, on di~til-lation gaYe 37.4 g. of product, b~p~ 74-79C./.2~
torr. -~7. 3-~S?-methyl-l-heptanol ~-To a solution of 74.8 g. of 5-~S)-methyl-7-(t-butyldimethylsilyloxy)-2-hepte~e in 500 ~1. of methanol was added 7.5 g. of 10% palladium hydroxide on car~o~ :
and the mixture sha~e~ in a hydrogen atmosphere for 1.5 hours at 50 psi. The catalyst wa~ filtexed a~d the solvent removsd under vacuum, 30 g.
.
E8. 3-(S)-methylhepta~oic ~ci~
To 10 g. of 3-(S)-methyl-1-heptanol in 175 ~1. of acetone was added over 45 ~inutes 90 ~1. of Jones reagent dropwise at 15-20C. Aft~r 15 ~inu~s, 15 ml.
of isopropanol was added ~nd stirri~g conti~ued ~or 30 minutes. The reaction was poured into water and the product extracted with ether. The extracts were - combined, washed with water, a sodium bi~ulfit~ ~olution and a brine solution and dried over mag~e9ium ~ulfate.
Removal of the solve~t gave 10 g. o~ the produ~t as a liquid, b.p. 84-88C./.~ torr, ~alphal~ 4.46 ~C~3O~
C=0.105 g/ml.).
E9. 3-~S~-methylheetanoyl_chloride Following the proçedure of Prepar~tion A2, 5.0 g. , of 3-~S)~me~hylheptanoic acid and ~.5 ~1. of oxalyl chloride gave 2.9 g. of the desired acid chloride, b.p.
29-32C./.25 torr~ ::
DD ' :
''' ;'.
:-.
The ~MR spectrum (DMSO-d6) showed ab~orption at 8.25-8.05 (m, 3~), 4.33-4.12 (m, 2~), 3.72 ~d, Js6~z, 2~), 2.21 (t, J-8~z, 2~), 1.88--1.68 (m, 1~), 2~0801.9 (m, 4~1, 1.28 (d, J~9~z, 3~) alld 0.~ ld, J~7~z, 6~) ppm.
Starti~g with the appropr:iate acid chloride and D-gamma-glutamyl ~alpha benzyl ester)-glycyl-D-alanine benzyl ester hydroehloride and employi~g the procedure of Exæmple lOD, the following compounds were pr~pared:
O .
RlCN~ \ / 2~
I D
~C~12) ;2CON~CH2CON~IIHS:0 ~3 '-:
R
~0 CH3(Ca2)8- >l~S I~SO-d~ 8.22 ~, 2h), 4.35-~13 tm, 2H), 3.83 (d, J-6~z, 2~3~ 2,22 ~t, 2~ 3~6~z, 2~), 2.1~ (t, J~6~z~ 2~) D 2.08-l.~S ~ 5 1~18 (~, 17~) and O.gO ~t~ J~7~z, 3H) -3.57~
:
Rl m. p . ~ ~ ~
(CH3) 2C}~- ~110 (dec) (D~5O~a6) 8 . 2-8 . 08 (~, 21~J, 8.02 (d, J- .
S g~æ, 1~ . 3-4 .1 (m, 2~), 3.72 Id, J~7~z, 2~), 2.53-2.40 lm, 1~), 2.~2 ~t, J~g~z, :
2~), 2.,10-1.7 (m, 2~) " 1.~8 ~d, J~9~z, 3~) ~Ad 1.08-0.9 .. (In, 6~) '~
~ ~110ld0c~ d6) B.3-7.9 (mt 3~), 4 . 33-4 ~ 17 (m, 2~), 3 . 72 (d , J~5~z, 2~1, 2 . 3-? .1 ,:~
(~ 2~) and 2 .10-1. 9 . Im, lS~) ;.
CH3(C~2)2- a~110(d2c) (~O-d6) 8.25-8.05 (~, 3~ . 35-4 . 22 :~
(~, 2~), 3.72 ~d, J~
~, 21I), 2.22 ~
.
J~ F~zo ~Pl), 2.12 (to J~ lz, 2~), 2 . 08-1 . 77 (~, 2~1, 1 . 55 .
lq, J~z , 2EI), ~ . 28 .
~d, J~ , 311~ and 0 u 88 (t ~ J~7i~z D 3E~
-'.
~' ' Rl - m.p. ~C.
._ C~3 ~C~2) 4- >190 Ide~3 ~D~S0-d~ .33~8.0 ~, 3E~), 4 . 35~
lla, 2E~), 3.7 5d, J~ -6~z, 2R~, 2.17 (1:, J~B~z, 2~1), 2.1 It~
1~8~ 2t 2~ 2.05- :
1 . 63 (~ 2~), 1 . ~8 ~t, ~7Elz , 2~), 1. 2-1.1 (m, 7E~) and 0 . 85 ~t, JSG7~Z t 3~) CE~3 ~H2)6- >180 5dec) tD~01 ~.42-~28 (m, 211) o 3r91 Is, 1~3, 2 0 4 ~ 7~z , 2131 ~ 2 a ;27 ( 1 ~ J27~1z, 2~), 2.22 1 . 94 Im, ., 2~), 1.65-1,55 (m, 2H) " 1.39 Id, J~8Hz, 3~) t 1.3~-1,17 (m, 8H) and 0 . 73 (m, 3EI) ' ~''~
,.. .
:
~, ' ---25- ;:
,c. 3~E~
~a3 ~3(CH2)3C~c~2~ 6) ~.~7~~-03 ~ , 3~), 4.32~
(~ 2~ ~ 3.72 (d, z , 2E~), 2 . 22 Elz , 2~, 2.27-1 . 68 (m, 6}11, lo 1.42-1~ 0 ~m, 101~1 aad 0 . 94-0 ~ 8 lm. 6EI) ~ 2CH3 15 C~3~C~2)3C~ S0--d~;) 8.22-8~0 ~,S~ ~, 3E~), 4.32~
(~, 2H), 3 . 8-3 . 6 (~, 2~[), 2 . 28-1 ~ 68 SB) " 1.6-1.0 ~, 12~ and 0 . 94O
~.7 tls, 6~.
(C~3C~2)2C~ SO-~6) 8.29~ 7.97 3~), 4 . 33-4 . 1 ~, 2E~3, 3.8~ 3.~9 (~, 2EI~, 2 . 32-1 . 65 ~, 6~) ,, 1.65-1.1?
(~D ~ ~d 1 . 02 -30 0.68 (m, 6~) :: `
'-'.
.
, ~2~7 - R
~C~2-- ~ 50-db ) 8 . 3-B 0 0 (~s 3~), 4 . 32-4 . 1 S ~, 2~), 3.85-3.62 ~3a, 2H), 2.21 ~t, J~8~x ~ 211), 2. 02 8Elz , 2~), 2 . 01-1. 9 (m, lE~
0 1.115 l.S ~r~, 8~, 1,Z8 ~d, J~8Hz, 3~1 a~ 1. 28-0 . 8 (m, SH
(CH3CH2CEI2 ) 2C}ICH~ ~5SO~d6 ) 8 .18-8 . O
n, 3~ . 31~4 . 1 ~ 2~) " 3. 84-3 .
(~ 2E~), 2 0 2~
J~6EI~, 2H) ~ 2.07 ~a, J~BlE~æ , 2H), :~
2,.03~1.7 ~m, 3~), 1 o 15 ~, llE~) ~d O . 87 ~t, J-6Hx, (CH3C~2 ) 2CE~CE12 (D~3S0--d6 ) 8 . 27~7 " 95 ~, 3H), 4 . 3 -~ . 1 (m, 2~3 ~ 307~-3.~ (~, 2~ . 3 1 . 57 (~, 8~), 1..46-1.13 8E~ a~d 0.84 ~t,, J~
8~x, 6 7~4~
:
Rl m. p . ~,~, ~ H3 C~13 (CEI2~ 3C~C~2 5D~SO-d6) 8 .18-8 . O ,:
S ~R, S) ~,, 3~, 4 . 2~--4 . 05 ~m, 2~), 3074-3.S6 ~m, 2H), 2017 5t, J~
9Elz , 2EI), 2 . 12-2 . 0 ~m, lEl), 2 . 0-1 i, 64 lo 5DI~ ~H~, 1 . 2~ (d, J-6~z , 7EI), 1 . 14-O 0 98 ~m, 2EI) ~nd 36Elz, ~) lS
CH3 (CH2) 2CHC~2 (D~IS0 d6~ 8 . 2-8 . 04 (R,S) (mg ~), 4.26-4.08 ~m, 21~, 3 . 76-3 .. 6 (m, 21I), 2 . 28-1 . 64 ~, 7E~ -O.g~
(~, '7~ ~nd 0 . 96-0.7~ ~m, 611).
.
' ~D ~
. .
:,:
:
. .
,~
.
j7~
-~8--Rl al~ ~ ,, H3 ) 2C~ tCH2 ) 3- ~ - 1~S0-d6~ 8 . 2 4-7 . 95 ~s, 3B), ~.3-4.08 l~, 2~1), 3 . ~1-3 0 59 ~Dl, 2~ ,. 21 (t, J-6~z, 2h), 2 0 11 (t, J~z ~ 2~31, ;
2.05 1.38 tm. 7EI) 9 ", l.,Z7 ~d, J~8~z7 3~) ,, 1.17-1.05 ~m, 2 a~ 0.86 ~d, J-lO~z 6~) c~3 (D~SO-d6~ 8 . 24Tr8 . 0 C~3 (CE~ CH- ~, 3H), 4 . 33-4 .11 iR95) ~m, 2~, 3 . 79-3 . 6 (~ 2~ 2 a 41--;! ~ 29 (1;~1, llE~), 2 ~ ;~2 ~t ~
J~8~31z~ 2~ 66 (SD., 2EI) ~ 1~59-1~43 t~ 1038-1~11 ;.
(m, 11~), 1.06-0.95 1~, 3~ and 0 . 87 ,~
~t, ~-6~z,, 3 .
, .
m.p.,~C
C~3C~2 5 CH3(C~23 3CHCH2 ~DI~SO-d6~ 8.24-8.02 (R,S) (m, 3EI), 4.32-4.11 ~m, 2~), 3 ~ 3 . 6 ~t 2~311, 2.24 ~t, ~gt~z , 2~), 2 . 08 : .
Id, J~lz , 2~, 2 . 03-1.,~3 ~ ), 1.4~-1.11 (m, 12~) and 0.97-û.71 ~m, 6H) 15 (CE13)2ClItc~2)~ D~IS0 d~j~ 8.24-8.û~
tm, 3~), 4.28-4.1 i~, 2E~, 3.76-3.6 2E~3, 2 . 18 (~
6~Iz, 2~), 2~1 tt, 6~z , 2~3, ~ O 04- ~ .
1.8~ , 1.84-1.66 (D~ , 1.56-1~38 Im, 3~), 1.23 (~1, 3~6Elz, 311) " 1.2-1, 06 a~, 3~ d ~.82 (~ J~6~{z, 6~) ~
., ::
C
C~3 (C~2) 4~ 2~ fiSO-d6) 3.23 7098 IR~S) ib~9 3J~1), 4,.3-4.13 ~, 2~), 3.~1-3~61 ~Q, 2fl), 2 . 22 (t, -;J5E~ 2~), 2 . 18 - ;:
1.68 ~, 6H), 1.45-1007 ~m, 12~l~ and 0.98-0.8 (m, 6~) .. :
C~3 (C~13)2C~(C~2~2C~c~2 (D~SO-d6) 8.37-8.03 ~
(R,S) l~, 3~I~ O ~.31-4.1 ~, 2H) 7 3.78-3.6 ~El, a~,, 2.26 (t, J~ z, ~, 2.~-1.36 l~, 7E~) ~, 1.3 Id, J~.
8~1z , 5~), 1 . 25~1 . 05 1~" 2}13 ~nd 1. 05-0.73 lm~ 9H) :
~5 :
,~
~ 3~
:
-31- ::~
';
Rl m.~!C. ~ :~
~E~3 ~~ IDMSO~d6) 8.23-7.94 C~3) 2CHC:H2CHC1~2 (Im, 3~1), 4 . 3-~ .13 1~, 2EI), 3.Bl-3.51 (D~, 2~1), 2.21 ~t, J-8Elg, 2~ . 15-2 . 0 t~, 2H), 1 . 9 (t, J~8E~z , 2~1), l . dS-1 . 52 Im, 3EE), 1~ 4--1~ ;!2 (1~1~ 3EIl r 1 ~ 2~2~0 ~ 94 (~ 3~) ~nd 0.94-0.80 (~, 6~
1~ .
C~3C~2 CH3~C}l2~2~HcE~2 IDMso-d6) 8.25-7.9 IR,S~ (m, 3EI~, 4.3~4.0~
Im, 2~), 3.81-3.62 Im, 23~), 2.22 (t, Js8~z ~ 2~1, 2 . 06 (d, J~81~2, 2~
. 02-1 . 89 (m, 1~, 1~ 87-1. 65 lm, 2~
1., 41-1. 06 (~, llH) ~d 0.~8-~.7 (D, 6E~) :
. ~
, ~0 , .
~.
7~
--3~-~1 m ~
O~d2- ~- (DISS0-~16) 8.33-7.9S
1~, 3E~ . 3-~ . ofi (~ 2~) ~ 3.83-3.59 :
~, 2~), 2.21 It, ~:
J~8H;Go 2H), 2.11 l~
3~), 2008-l.a7 ~
1 . 87-1 . 3~ (~, ~B), 1 . 25 ~d, J~88z , ,~
3~ d 1. 22-0. 98 3~
16 (CH3C~2 ) 2C~ ~CR2 ) 2 (1~S0--d6 ) 8 . 21-8 . U
(~ 3~ . 32~
i~n, 2E~), 3.83-3.6 l~, 2~), 2.21 (t J~8~z, 2E~1, 2.11 ~t~ J~ I8, 2H) ~
2~05-1.89 ~m, 1~3, 1.87-1.67 5m, 1~) 0 l.S7-1.38 Im, 3~[3 g 1 . 38Q1. 08 ~m, 9~1 ~d 0.83 It, Jz~6~z~
6Elj , , ' ', .
.
.
~2~7~3~
l ~3 C~3 IC~2) 2CE~ ~C~23 2- ~ ~ DlllSO-d6~ 8 . 24 7 . 97 (R,S) ~3~, 3~), 4033~4.1 (m, 2~), 3 . g6-3 . 59 (~, 2~), 2. 35-2 . 08 ~n, 4~13, 2 . 08 1 . 9 (Dl, 1~), 1 . 89-1 . 67 (~ 1}~), 1.~3-1.46 1 0 416-1 . 02 im, llB~ and 0 0 98-~o73 t~, 6EI) C~3~2 CH3 (C~2l ~,C~C~2 ~S0Wd6~ 8 .2-7 . 94 ;~.
(R,S~ t~, 3E53 0 ~.26~4.06 ~, 2~, 3 . 7~-3 . 56 .
a, 2~[), 2. 16 (t, J1~6~, 2~), 2,.1-l. B4 (~Q, 31~), 1 . 84-1 ,. 6 (~, 2~1), 1 ~ 24 (d, J~
6Bz, 9H), ï.12 0.~2 :;
26 t~, 3~ 9.92~ :
0.64 .- :
~ . ~
- :~ 2~ ;7~
:
C~3 3 (~ ) 5C~C~2~ MSO-d6 ~ 8 . 23-8 . 0 lR,S) (211" 3~), 4.32--~.,06 (~, 2~) ~ 3.72 ld, J~ , 2~), 2 ~ ~2 (t , J~lOHz , 2B), 2 . 16~ 611), 1. 42-1. 08 (m, 14~1 ~nd 0 . 92~7 . 0 (m, 6~) C2~5 16 c~3lC~2)3c~c~2 (D~qSO-d6) 8.2-8.0 (S) ~ay 3~ . 2~-4 . 1 l~, ;~1~3[) t 3.7~ 3.60 5m, 21~), 2 . 18 ~t~
J~7, 2~), 2.02 ~d, J~7, 2H~, 2 . 02-1. 6 - (~, 3~1, 1.26 ~d, J~6 , 3EI), 1 . 26-~ . 08 5~Q" 8El) and . 92-. 74 (~, 6HI
, 3~ . .
.
- 35 - ~ 5~ 72222-8 N-(3-(S)-Methylheptanoyl)-D-gamma-glutamyl-L-alanyl-D-alanine (Rl = (5) CH3(CH~)3CH(CH3)CH2-;
R2 = CH3, R3 = ~NHCH(CEI3)CO2~) 12A. N-t-butoxycarbonyl-L-alanyl-D-alanine benzyl ester To a solution of 23.0 g. (0.121 m) of N-t-butoxycar-bonyl-L-alanine, 42.6 g (0.121 m of D-alanine benzyl ester ~-toluenesulfonic acid salt and 17 ml. (0.121 m) of triethylamine in 400 ml. of cold (0C.) methylene chloride was added dropwise 25.0 g. (0.121 m) of dicyclohexylcarbodiimide in 100 ml. of methylene chloride. After stirring overnight at room temperature the solids were filtered and the filtrate concentrated to an oil. The resi-due was dissolved in 400 ml. of ethyl acetate which was washed with a 1% hydrochloric acid solution, a 10% potassium carbonate solution, water and a brine solution. The organic phase was separated, dried over magnesium sulfate and concentrated to an oil. The residue was triturated with diethyl ether and the resul-ting solids filtered under nitrogen, 16.0 g. An additional 12.7 g. of the desired product crystallized from the filtrate.
12B. L-alanyl-D-alanine benzyl ester hydrochloride To a slurry of 28.7 g. of N-t-butoxycarbonyl-L-alanyl-D-alanine benæyl ester in 150 ml. of dioxane was added 150 ml. of dioxane saturated with hydrogen chloride and the mixture stirred for 4 hours at room temperature. The solven-t was removed ln vacuo and the residue triturated with diethyl ether. The resulting solids were filtered, redissolved in methylene chloride and the solution concentrated to about 150 ml. Ether was added and the solids filtered under nitrogen, 22.0 g.
12C. N-t-butoxycarbonyl-D-gamma-glutamyl (alpha benzyl ester)-L-alanyl-D-alanine benzyl ester To a slurry of 5.0 g. (9.64 mm) N-t-butoxycarbonyl-D-gamma-glutamine alpha benzyl ester dicyclohexylamine and 2.76 g. (9.64 mm) of L-alanyl-D-alanine benzyl ester hydrochloride in 100 ml. of methylene chloride cooled to 0C. was added 2.0 g. (9.64 mm) of dicyclohexylcarbodi-imide in 20 ml. of the same solvent. After stirring overnight at room temperature, the solids were filtered and the filtrate concentrated ln vacuo. The residue was treated with 150 ml. of ethyl acetate, the solids filtered and the filtrate washed with 1%
hydrochloric acid, a 10% potassium carbonate solution, water and a brine solution. The organic phase was dried over sodium sulfate and concentrated to give a white solid, which when triturated with ether and filtered gave 4.1 g. of the desired product.
12D. D-gamma-glutamyl (alpha benzyl ester)-L-alanyl-D-alanine benz~l ester hydrochloride To a slurry of 4.1 g. (7.21 mm) of N-t-butoxycarbonyl-D-gamma-glutamyl (alpha benzyl ester)-L-alanyl-D-alanine benzyl ester in 50 ml. of dioxane was added 100 ml. of dioxane saturated with hydrogen chloride, and the reaction mixture stirred for 3 hours at room temperature. The solvent was removed under vacuum and the residue triturated with diethyl ether, 3.5 g.
~r .:
12~. N-13 (S)-~ethylheptanoyli-D gamma-gluta~yl (alpha To 1.0 g. (1.98 ~ml o~ D-qamma-gluta~ lpha benzyl ester)-L-alanyl-D-alani~e benzyl ~ster and .833 ~1. ~5.93 mm) of triethyl amin~ in 50 ~1. of ~ethylene chloride was added 390 mg. (2.37 mm) of 3-(5)~methyl-heptanoyl chloride and the reaction ~ixture stirred under nitrogen for 45 ~inutes. ~he reaction w~s poured into 150 ml. o~ ethyl acetate ,and the organic phase wa~
washed with 10~ hydrochloric acid, ~ 10% potas~ um carbonate solution, water and a brine solution. The organic phase was dried over sodium ulfate and conce~-trated to dryness. The residue was triturated with ether and filtered under nitrog~n, 900 mg.
12F. N-~3-(S~-methylheptanoyl-D-gamma-glutamyl- .
L-alanyl-D-alanine A mi~ture of 200 mg. of palladium hydroxide on car~on and 900 mg. of N-~3-(S)~methyl~eptanoyl)-D-gamma- ¦
2D glutamyl ~alpha benzyl ester~-L-alanyl-D-alanine ben2yl ester in 50 ml. of methanol was sh~ke~ in a hydrogen I :
a~mosphere at 5~ psi for one hour, The catalyst was filtered and the solvent removed in vacuo. Water was added to the residue and removed under reduced-pre~sure to give ~92 mg. of the product as a white solid, m.p~ ¦
165-168C.
The N~R spectrum (DMSO-d6) showed a~sorption at 8.21-7.98 tm, 3~), 4~41-4~1 tm, 3~j, 2.3-2006 ~, 4~0 2.06-1.56 lm, 6~), 1.43-1.02 (m, llH) and 1.02 0.73 (m, 3~ 6~) ppm7 N-(3-(S,R)-Ethylhexanoyl)-~ ga~ma-glutamyl (alpha n-butyl estes)-glycyl D-alanine ( 1 C~3~C~z)2C~(c2~s)c~2~; R2 ~ H;
R3 - -N~ ~(CH3)CO~ C~
J -~ C~ y /
13A. N-t-butoxycarbonyl-D-gan~a-gl~t~Din~~~alpha A solution of 39.5 g. I0.,172 m) of N-t-bu~oxy-1~ carbonyl-D-glutamic anhydride in 75 ml. of dry tetra-hydrofùran was added dropwise o~verba~at~ol hour period to a solution of 4~ ml. (0.516 m~ and 34.3 ~1. (0.172 m) of dicyclohexylamine in 300 ml~ of ether at 0C. The reaction was allowed to ~tir at 0C. for 3 hours and ~as 15 . stored in a refriger~tor overnight. ~he solids were filtered, slurried in ethanol and filtered, 43.3 g~
13B. D-gamma-gluta~yl (alpha n bu~yl ester)-glycyl-~ h~ product of Example 13A tlO g.~ 0.021 ~ and 6.7g. (.024 ~) of glycyl-D-ala~ine ben~yl este~ hydro-chloride were slurri~d in 200 ml. of methylene chloride under nitrogen and cooled to 0C. Dicyclohexylcarbodi-imide (4.25 g., .021 m) was added and the ~ixture allowed to warm to room temperature overnight. The urea byproduct was ~ilte~ed and the solv~nt re~ov~ in vacuo.
The residue was treated with ethyl acetate and filtered~
The filtrate was washed succes~ively with water, 2.5%
hydrochloric acid, water, 10% potas6i~ carbonate and brine. The organic phase was dried over magnesium sulfate, the solvent removed under vacu~m and the ~,Z~ j7 '~3 L~
, residue dissolved in 300 ml. of dioxa~e ~aturated with hydrogen chloride. After stirrin~ 4 ho~rs at room temperature, the solvent ~as removed ana the residue triturated in ethyl ac~tate-h~xane (1:1) and filtered, 7.4 g.
13C. N-(3-(S,R)-ethylhexanoyl-D-glutamyl(alpha n-but~1 ester)qlycy~-D-alanine _ __ To the product of Example 13B (1.0 g., 2.35 mn) and .99 ml. (7.05 mm) o~ triethylamine in 50 mlO of I0 methylene chloride was added 460 mg. l2.83 mm) o~
3-(S,R) ethylhexanoyl chloride and the reaction ~tirred overnight under nitrogen. The solvent ~ai~ re~oved in vacuo and the residue dissolv~d in ethyl acetat~. The 16 organic phase was successively wash~d ~ith 10%
hydrochloric acid, water 10% potassium carbonat~ and .
brin~. The organic phase was separated, dried vver ~agnesium sulfate ~nd the solvent remoYed under vacuum. :
Th~ residue was dissolved in ~0 ml. of emtha~ol and shaken with 170 mg. of 10~ palladium hy~roxide in a hydrogen at~osphere at an initial pres~r~ o~ 50 psi for 1.5 hours.
The spent ~atalyi~t wa~ filtered and th~ solvent removed in vacuo, 100 mg.
NMR ~D~SO-d6): 8.18 (d, J - 6, 1~ o 8.10 (d, J=6, 1~), 8.02 (t, J-5, 1~), 4.28-4.10 (m, 2~ .09 it, J-6, 2H), 3.78 3.56 Im, 2~), 2.18 St, J 6, 2~), 2002 (d, J=6, 2~), 2.00-1.60 Im, 3H), 1.58-1042 Im, 2~), 1.28-1.08 (m, 8~, 1.24 ld, J=6, 3~)~ 0.92-0.76 Im, 9~).
: 30 ~S~
EX~MPLE 1 4 Employing the general procedure of Example 13, and starting ~tih the requisite reagen~s, the ollowing s:ompounds were prepared:
O
RlCN~ ~ I) ~ C2R4 fH D
C O ~CH2) 2CONE~CE~2CON~CEI(C~3~2 ~ R4 D~R
3 -methylheptanoyl methyl Nlq~ (DMSO-d6 ): 8 . 23 ~d , J~ , 1}1), 8 . 15 (d, J -6 , 1}1), 8 . 03 (t , J~6 , 11~), 4 . 28-4 . 1~ (m, 2E~); 3 . 72 ~d, J-6, 2E~), 3.61 (s, 3~ 3 ~t, J-7 , 2~), ~.16-1.70 Im, 6EI), 1.34 loû4 (~a, 8~1) t 1.25 ld, J~75 31~) 9 o.s2;-o.?~ Sm, ~:
6~).
.
. .
-.~2~7~
.J~ ~/ R,a N~fn 3-ethylheptanoyl methyl NMR (Dl~SO-d6~: 8.19 (d, J~6 , 1~), 8 . 1 1 ~d , J~6 , 1~, 8003 ~, J-6, 1~
4 . 2~-4 . 10 ~m, 2~), 3 . 74- : ' 3,62 ~, 2~), 3.57 I~.
3~, 2.18 (t, J-9, 2H), 2.01 ld, Js6~ 2~1, 1.97~
1 . 60 ~m, 3~), 1 . 32-1 . 10 ~m, 88), 1 . 23 (d, J-7 , ;
3EI), 0.90-~.72 (~, 6~) :
ll . 3-methylheptanoyl ethyl N~R ID~SO-d6) s 8.. 248.n2 (m, 3~ .26-3.56 (m, ~ ~ .
~, 4.04 (q, J'9 3.76-3,56 ~m, 2~) ~ 2~17 ;
(t, J~7, 2~ . 12-1 . 63 (m, 6~); 1 . 74-0 . 9d, ~m, 6~), 1.23 (d, J~5, 3~), 1.13 ~:, J~9, 3~;, 0.8~-0 . 72 (m, 6~3 ~ ;.
' 5~
, '~ R, c o .~ 4 ~R
3-ethylhepta~oyl ethyl NMR (D~SO-d6): 8.16 (d, J#6, 1~), 8.09 ld~ J~6, ~ 8~02 ~t, J~6, 1~), 4.~2-4.08 lm, 2~), 4.D2 (q~ J~7, 2~), 3.7~-3,54 ~m, 2~3, 2.1~ (t, J-7, ~:~
2~1, 2,00 (d, J~6, 2~j, 1.96-1O58 (m, 3~), 1.30-1.08 (~, 3~), 1.21 (d, ~'7~ 3~) t 1.13 (t, J~7, 3~)~ 0O88 0~70 (m, 6~) 3-methylheptanoyl iso- NMR tDMSO-d6): 8.14 (d, bu~yl J~6, 1~), 8.08-7.98 lm, 2~), 4.20-4.04 (m, 2H)~
3.75 (d, JY6~ ~), 306B- :
3.54 (m, 2~), 2.14 ~, J~6, 2~ .08~ t 1.28-0.96 ~, 6~), 1~19 Id, J~7, 3~, 0.88 0.70 ~m, 12~) as 1":, .
~ ' '.
:
: ' ' '',.
'' .'' , .'~
~ ~ R ~
__ .
3-ethylheptanoyl iso- ~MR tDt~lso; dc): 8 .21 (d, butyl J~6~ 1~) t 8.1~-8.04 (m, 2B), ~ . 24-q . 08 ~, 2El), 3-79 Id, J-6, 2E~1 I 3.72-3.58 (m, 2~1), 2.19 (t,.
J~7, 2B), 2.03 5d, J-6, 28), 1 . ~9-1 . 6~ (~, 4~
1 . 32-1 . 10 ~, 81~), 1 . 23 (d, J-6, 3EI), û.g~ 0~72 Im. 12~) 16 3-ethylh~xanoyl isc>-- NMR (DNSO~-d6). 8.18 (d, butyl J=6 , lH~, 8 . 10-8 . 00 (m, 2~) ~ 4.26-~.08 Ir~, 2~I), 3.79 (d, J~6 , 2~33, 3~,72-3.58 Im, 2H1, 2.18 tt, J~6~ 2~), 2.02 ld, J~6, 2B), 1.98-1 . 62 ~m, 4E3 ), 1 . 3q-1. 08 (mO 6EI), 1 . ~3 ~,d, J~7; 3~), 0.96-0.72 (m, 12 ,,~.
, :, '' . .
, .
i7~3~
~ i -44~
-''' R,~-C
3-ethylhexanoylmethyl ~R (D~IS0~63: 8.21 ~d, J~7, 1E13, 8.10 (d, J~7, 11~), 8 . 05 (t, J~6 , lH~, 4.,26-~ .10 ~m, 2H), 3.76 3.~0 (~, 2EI~, 3.5g (~, 3~), 2.18 (11:, J~6, 2~
2.02 (d, 3~6, 2H), 2~0û
1 . 60 (~, 311), 1. 3~ 1 . 08 (~, 7EI), 9.90-~.72 (~
611) .
3-ethylhexanoyl ethyl pa~qR (D~S0~6) 5 8.22 (d) J~7, 11~ " 8.l8-a.~6 (m, ~3, 4 . 26-4 . 10 (m, 2~), 4,.06 Sq" Js5, 2Hl, 3.7~
3 0 58 ~m, 2E11~ 2 . 20 (~ , J~6 , 21~1, 2 . û4 Id , J-6 , 21I~, 2.02-1.60 Im,, 3H) 0 1~26-~.20 (~3, 7~I~, 1.18 t , J~5 , 3~1~, 0 . 90-0 0 7 ~o ~13 .
:io `
57~3~
~!
--~5--O
R4 ~R
__ __ 3-~ethylheptanoyl butylNMR ~DI~ d6): 8 . 20 (a, J~7, lE~ ~ 8.16 B.04 i;o, 2~1), 4 . 24-4 ~, 0S ~m, 2EI), 4.00 (t, J~6, 2H), 3.7~-3 . 56 ~m, 2EI~, 2 . 17 ~t, J~6 , 2}1), 2 . 12~ 0 ~, !
5}~ 1.58-1.40 ~m, 2~
1.36-l.00 ~, 8~), 1.21 (d~ J-6, 3~), 0.90-0.7~ :
(m, 9EI) 3-ethylhep~a~oyl butyl N~R ~I)IqSO d6): 8.16 ~d, J~7, lEI) " 8.11 (d, J57, 1~, 8.03 ~, J~5, 1~, 4.26-4.09 ~m, 20~1), 3.99 (t , ;1~7 , 2~I~, 3 . 79-3 . 58 (m, 2H), 2.17 (t, 3~6y 2}1~ 2.01 ~a, Js6, 2~), 2.00-1,,60 ~m~ 3E[) ~ 1058-1.42 I~, 2~11, 1.36-1.08 (~, lOEI) ~ 1.24 (d, J-S, . :
3~, 0.92-0,,72 (~, 9El) !
.
,:
.
.
~ 7~3~
~6-__ N-~3-(R,S)-Ethylhexanoyl~-D-gamma-yl~tamyl glycyl-D-alanine ethyl ester ~Rl ~ C~3(C~212C~C2H5)C~2 ;
R ~ ~; R - -~NC~C~I3)C02C~
S 2 3 ~ 5 15A D-gamma-glutamyl (alpha benzyl ester)glycyl-D-alanine ethyl ester h~d~ ~
To a ~lurry of 14.8 y. ~.0285 ~) of N-t-butoxy- :
carbonyl-D-gamma-glutamic acicgl alpha benzyl e~ter dicyclohexylami~e salt acid ~ . V285 ~) o glycyl-D-alanine ethyl ester hydrochloride in 200 ml~ o methylene chloride was added 5.6 g. ~0270 m.~ o~
dicyclohexylcarbodiLmide an~ the mixt~re ntirred under a nitrogen at~osphere overnight. The ~rea i~ filtere~ and the solvent removed in acuo. The r~sidue was treated with 300 ml. of ethyl acetate, filtered ~nd the filtrate washed successively with 2.5% hydrochloric acid, water, 10% potassium carbonate solution and brlne. The organic phase was separated, dried over magnesium and concen-trated under vacuum. ~he residual oil ~as dissolved in 450 ml. of dioxane saturated with hydrogen ~hloride.
The ~olution was stirred ~or 2 hours and the solvent removed in vacuo. The resi~ue was triturated with ether :
an~ filtered, 11,2 g.
15~. ~(3-~R,S)-e~hylhexanoyl)-D-gam~a-glutamyl- :.
~lvc~l-D-alanine ethYl est~r :
. , ~ _ _ , . . .
To the product o Exa~ple l5A 11.0 g., 2.33 ~
and .98 ~ 6..98 mm.,) of triethylam~e in 30 ml, of methylene chloride, under a nitrogen atmosphere, was added 378 mg. ~2.33 ~.. 3 of 3-(R,S)-ethylhexanoyl chloride. Aft~r stirring at room te ~ :rature for 1.5 hours the mixture was poured into 100 ~1. of ethyl .... . . ..
_ 47 _ ~ ~ S 7~ ~2222-8 acetate and the organic phase was washed successively with 10%
potassium carbonate solution and brine. The organic phase was separated, dried over magnesium sulfate and concentrated in vacuo.
The white solid residue was dissolved in 30 ml. of methanol and hydrogenated over 0.1 g. of palladium hydroxide in a hydrogen atmosphere at an initial pressure of 50 psi. After 2 hours the catalyst was filtered, the filtrate concen-trated to dryness and the residue triturated with ether and filtered, 275 mg.
NMR (DMSO-d6) 8.26 (d, J=9, lH), 8.14-8.02 (m, 2H), 4.31-4.00 (m, 2H), 4.06 (q, J=10, 2H), 3.78-3.60 (m, 2E), 2.17 (t, J=8, 2H), 2.08-1.65 (m, lH), 2.03 (d, J=8, 2H), 1.82-1.53 (m, 3H), 1.40-0.96 (m, 5H), 1.23 (d, J=6, 3H), 1.14 (t, J=10, 3H), 0.90-0.64 (m, 6H).
Starting with the appropriate reagents and employing the procedure of Example 15A-15B, the following compounds were pre-pared:
O
RlCNH \ D / C02H
CH D
(CH2)2CONHCH2CONHCH(CH3)CO2Rs X
~2~ 34 --48-- ;
,O
,~, , , 3R5 ," ,NM ... t. "
3-methylheptanoyl iso-butyl MI~R (Dt4SO-d6): 8.24 ~d, J~6 , 1~), 8 . 1û-8 . 00 ~m, 21~ . 30 ~ .18 (m, . O~ ~m, 1 3 . 86-3 . 72 (~n, 2H~, 3.72- 3.58 ~m,, 2~), Z.16 ~t, J-6 , 2El), 2.12--1~64 ~, 6~I1 D
1.52-1. 00 Im. 6~
1 . 27 ~d o J~7 ~, 3El), 0.90-0.76 (m, 12~) 3-ethylhexanoyl iso-butyl ~R ~I:)MSO-d6): 8 . 23 (d, J~6, lEI) " ~.0~7.99 ~mg 21~), 4.29-4~17 ~m, lEI), :
4 . 17-~. 07 (~, 1~), 3.83-3.71 ~m, 2~), 3.71-3~5$ (m, 2El) ~ 2.15 .
(t, J~7~ ~), 2.0~-1.60 ~gl, 4~), 2.00 ~d, J~6, 2~3, 1.31-l~Og Im, 6~1), 1 . 25 5d, J~6 , 3~), 0.90-0.72 (m, 12~I~
-.2~3~7~3~g, ;
-49- .:
0 R5 N~
3 ethylheptanoyl iso-butyl NMR ~D~SO~d6): 8.23 (d, ;
~8~ lM), 3.08-7.98 ~m, 2~) ~ 4~29-~ol8 tm, 1 4.18-4.07 (m, 1~), 3.86 3.72 (m, 2~, 3.70-3.57 tm, 2~, 2.15 (~ J-7, 2~), 2.04-1.5g t~, 4~), 2.00 (d, J~6, 2~), 1.30-1.11 (m, 8~), 1~25 ~d, J36, 3H), ~.89l0.70 (~ 9 12H) lS c~c/oh~ ne7~
3-methylheptanoyl ~ }- NM~ ~D~SO-d~): 8.25 (d, ~ye~he~t~ J~6~ 1~), 8.13-8.00 ~m, 28~, 4,32-4.20 (m, 1 4~20-4.08 ~m, lH3, 3.91-3.76 (~, 2~), . 76-3.5~ (m, 2~), 2.1 ~tJ J~ ), 2.13-1.48 .
(m, 8~), 1.36-1.01 Im, 12~), 1.27 ~d~ J56, 3~), 1.0~ 0.76 t~
50- .
R~ CO ,,~
3 ~}~ ~5 ~_ , . .
~)~Clo~< y/~n ~f~y/
3-ethylhexanoyl -a~L- NMR ~D~SO-d6~: 8.~5 ~d, _4~4~L~h~ J~6, 1~), 8.13-8.09 (~, 2H), 4~32-4~20 (ffl, 4.20-4.08 ~m, 1 3.9~-3.7~ (~, 2~
3.74-3.59 ~m, 2~), 2.18 tt, J~6, 2H3, 2.09-1.86 (m, 1~), 2.03 (d, J~6, 2~), 1.82-1.43 ~m, 8~
1.36-I~01 (~, 9~), 1.27 (d, ~6, 3H), l~01-0.70 l6 (m, 8 ~yc/~e~ y/h~ y/
3-ethylheptanoyl -met~y~ NMR tD~SO-d~): 8.26 ~d, h~hexrl- J-6, lR), 8.12~8.02 ~m, 2~ .31-4.1~ (m, 1~), 4.19-4.0~ ~m~ lH), 3.93-3.7~ (~, 2~), 3~72-3~58 (~, 2~, 2.18 ( , J~6, 2H), 2.08~1.86 (m, lH), 2.03 2S (d, J~6~ 2~)o 1.82-1.48 (m, 8~), 1.34 1.02 ~m, 11~), 1.27 6~, J36, 3~ 00 0~7~ lm, 8~ :
:
. .
;i7~
(` 0 ~ ~, R5 ~MR
.; . , .-3-methylheptanoyl ethyl N~R (D~S0-d6): 8.25 (d, J~6, 1~), 8.12-B.00 (m, 2~), 4.28-3.96 ~m, 2H)~
4.03 ~q, J~7, 2R), 3.74-3~56 ~m, 2~), 2.16 (~, J~9, 2~), 2.11-1.62 0 Sa9 6~), 1.32-0.98 ~m, 6EI), 1 . 24 ~d , J~7 , 3H), 1.14 ~t, J-7, 3H), 0.88-0~76 ~, 6~) 3-ethylheptanoyl e~hyl ~MR ~D~S0-d6~: 8.28 ~d, J~6; l~l, 8016-8.04 ~m, 2~), 4.32-4.04 ~m, 2H)~
4.10 (q, J36 9 2~, 3.~8-3.6~ ~, 2~), 2.22 (t, J-6, 2~), 2.11~1.92 ~ 3, 2.07 (d9 J 6, 2~ 1.8601064 S~, 2~
1.~0~ m, 8~), 1030 (d, J~69 3~), 1.21 (t, ~J~6, 3~), 0.94~0.7 2~ (~, 6~
i7~
.
R/_~D
R5 ~MR.
-- ;
3-ethylhep~ar~oyl l~utyl N~IR tD~IlSO~d6): 8.27 Id, J~8 , 1~1~, 8 ~ . 02 (al, :
21~ ,.32~4.1û (m, 2El), ~l i lO-3 . g~ ~, 2}I), 3.7a 3.60 ~, 2~I), 2.18 It, 3~6 , 2~, 2 . 0~a ~d, J-~ , 2E~, 2 0 0~-1 . 62 3~), 1060-1.46 Im, 2 1.38-1 . 10 I~, 12~
1 . 27 ~d , ~-6 , 3}I), 0~,90 0.75 Im, 9~1) 3-S-~et~yll~eptanoyl butyl W~ (DMSO-d6): 8.30 (d, J~8, 111), 8.15-8.04 ~, 2Ba, ~.~S-~.12 ~, 2~), !
~L r l 2 3 ~ 9 ~
3078-3~65 (~ 2~ 2~22 (tJ J~7~ 2EI) ~ 2~18-1~69 .-~" 7EI), l.G1~1.48 (~, 21~), 1.~0-1.11 (m, llB), 0 . 97-O . B0 (m, 9 30 - . ., ~5i7~3~
, ` . ~ ,.................................................... ..
~ 5 NMR
3-S-ethylheptanoyl butyl N~ ID~SO d6): 8.22 (d, J87, ~, 8.12~.0 ~, , 2H), ~ 6 (m, 2~, 4.08-3.95 ~m, 2 3.75~3.~2 t~
~t, J~6, 2~), 2.02 ~d, J~6~ 2~), 2.04-1.6 3~), 1.6~-1.46 I~, 2~
1.38-1~1 1~, lS~), and .9_.75 ~m~
. :
lS 3-ethylhexanoyl butyl NMR tDMSO-d6): 8~28 (d, J~8, 1~), 8.14-8.04 Im, 2~ .34~4.10 (m, 2H), 4.10-3.95 (~, 2~
3.75-3.62 Im, 2~), 2.19 2~ (t, J~6, 2~), 2.04 (d, J~6, Z~), 2.04-1.6~
(~, 3~), 1.60-1.45 Im, . .
2~), 1.40-1.10 (m~ :;
13~), 0.90-0.76 (m, g~) ;1 2~ 1 '~,.
i ~
--5~
EXAI~PLL 1 7 The procedure of Example 15 i~ a~ai~ ~epeated, starting with the appropriate reagents, ~ith the exception that the hydrogenation i8 not casried out, to give the follo~in~ compou~ds:
Il . 1' RlCM~ ~D ~ C02R4 ;:
C~[ D
'1~23 2CONHC:E~2CON;E~ C~3~c2R5 ,;
O ,:
~;;` ~ R R5 NMR
3-ethyl- butylbutyl N~R (DMSO-d6): 8.27 (d, J~7, hexanoyl 1~), 8.20 ld, ~7, 1~), 8.07 (t, J~70 1~ .37-4.13 (~, 2~3, 4.0~ (t, J~6, 4~), .
3.80-3.62 Im, 2~), 2.20 ::~
~t, J~6, 2~, 2.n5 (d, J-6, 2~), 2.92-1064 (m, 3~1, 1.60- :
1.47 (m, 4~3, 1.40-1.13 (m, ..
13~), 0~95-0O~7 (m, 12~3 3-ethyl- butyl butyl NMR ~DMSO~d63: 8.27 ~d, J-7, heptanoyl lH); 8.20 ~d, JD7Y 1~3 ~ 8-06 (t, J~6, 1~, 4.36-4.13 (m, 2~), 4.02 (~, J-~, 4~), 3.80-3.6q (m~ 2~), 2.20 - l~, ~=6, 2~, 2.04 (~, J=6, .
2H~, 2.00-1.60 g~, 3~) t 1O60-1.~3 (m, 4~3, 1.40-1.10 ~, ~ t 0.95-0~72 (~, 12H3 -3571~
,~$~ o _55_ .
~1 R4 R5 N~gR
3-methyl butyl butyl N~IR (DMSO-d6~: 8 . 26 (d, J~7 , hepta~oyl 1~ . 19 (d, J~7 , lEI), 8 . 07 ~t, J~6; 1~), 4.32-~.11 lm, 2~ . 02 5~ 0 J~5 t 4EI), 3 . 79-3 . 59 ~m, 2R) O 2 . 20 (t, J-6, 2~) f 2.14-1.68 Im, 5H7, 1 . 61~ .6 (m, 4H~, 1 . 40 -1 . 06 Im, 13~), 0 . 95-0 . 81 (m, 12~I) 3-S benzyl benzyl NMR (DMS0-d6): 8 . 33 td, J~7 , 15 methyl~ , 8 . 24 ~d, J~7 , 1~), 8 . 08 heptanoyl (t, J-5, 1~), 7~33 (s, 10~
5.08 ~s, 4H), 4.40~4022 (mt ~}~), 3.~û-3.60 ~ , 2.2 (~, J-5~ 2~), 2.14-1.64 ~m, 20 5~I), 1 . 26 ~ 7, 3~), ;
1.22-0,98 ~m, 6El), 0.88-0.73 (~, 6~1 ..
;
:j, ~,,, , ':
2~ ~7 ~ ~ ~
EX~MPLE 18 Crystalline ~-(3-tS)-methylhep~anoyl)-D-gamma-qlutamyl-qlycyl-D-alanine N-(3-~S)-Methylheptanoyl)~D-qamma glutamyl (~lpha benzyl ester)-glycyl-D-alanine benzyl ~ster (30.8 g.) was slurried in 300 ml. absolut;e eth~nol ~n a 2 liter autoclave. 5~ Pd/C, 1.54 g., S0~ water wet) was added and ~he ~ixture hydrogenated at: 4 x atmosph~ric pressure for 1 hour, by which time uptake of hydrogen was I complete. The catalyst was rec:o~ered by filtratio~, fir8t over paper, then over 0.~5 ~icro nylon ~ilipo~e, employing 100-150 ml. ethanol or tran~fer a~d wa6h.
The com~ined filtrate and wash liquors we~e stripped to 1~ a damp, white solid, which was di~solved ~n a 150 ml.of a hot, 1:10 mixture of ab~olute ethanol and acetonitrile, clarified by hot filtration, boiled down to 35 ml., 810wly cooled to ro~m temperature, granulated and fil~ered to yield crystallin~, den~e, non-electro-static tit~le product, 20.1 g. (94~) characterized by it8 ir (~u~o~ mull) ~hich includes major, well-r~olved, sharp peaks at 3340, 3300, 2900, 2836, 1725, 1650, 1628, 15B0, 1532, 1455, 1~10, 1370, 1280, 1240, 1276 and 1175 Cm~l This crystalline product (9.4 g) was further purified by dissolving in 1000 ml. of acetone at reflux for 1 hour. The solution was cooled to room temperature and seeded with a trace of the above cryctals. Afte~
stirri~g for S hours, title pro~uct was recovered by filtration with minLmal acetone wash, and dried ln vacuo at 35C., 7.25 ~., having identical ir characteri~tics.
p~
, 7~
-~7- :
N-(3~ Methyl-~-heptenoyl~-D-g~mma-glutamyl (al~ha benzyl ester~-~lyc~l-D-alani~e be~ l ester Following the procedure o:E ~xample lOD, 2.77 g. (5 mm) of D-gamma-glutamyl ~alpha benzyl e~ter) ;~lycyl~
D alanine benzyl ester hydrochloride an~ the acid chl~ride prepared ~ro~ 747 mg. (5 mm) o~ 3-(Rj-methyl-4-heptenoic acid gave the titled co~pound.
XA~PLE 20 N-(3-(S)-~ethyl-4-heptanoyl)-D-gamma~glutamyl-cllYc~l-D-al.mine A mixture o~ 500 ~g. of the product from Example 19 and 26 mg. of 5~ palladium-on-charcoal ~50~ water wet) in 125 ~1. of ethanol was shaken in a hydrog~n atmosphere at an initial pressure o~ 4 x atmospheric pressure for 2.5 hours. The cataly~t was iltered and the solvent removed i~ vacuo. The produ~t was purified by the procedure of ~xample 18, and was identical in all 2~ respects to the prod~ct of that exa~ple.
, .:
2~ ~''' ,::
. ~ ~ .. ~ .. . . . . . . . .
-:
i7~
:' PREPARATIOR A
Al. ethyl cvcl~ e-e To 4.9 g. of 60~ sodium hydride in oil wa~ ~dded sufficient hexane to di~solv~! the oil. To the oil free sodium hydride under nitrogerl ~as added 100 ml. of dry tetrahydrofuran followed by ~l solution of 22.2 ml. of triethyl phosphonoace ate in 80 ml. of dry tetrahydroD
furan. After stirring at room te~perature ~or one hour 10.5 ml. of cyclohexanone wa~ added i~l ~0 ml~ o~ tetra-hydro~uran and the reaction ~ixtur~ ~tirr~d at room temperature overnight~ The reaction ~ pourad into water and extracted with diethyl ether. ~he or~anic lS phase ~as wa~hed with lN sodium hydroxide ~oluti~n, water and brine. The organic phase was sep~ra ed, dried over ~agnesium sulfate and concentrated under reduce~
pre~sure .
~he residue was dissolved in 250 ~1. of ~ethanol, 23 trea~ed with l.S g. of 10~ palladiu~ hydroxide on carbo~ :
and the mixture ~haken in a hy~rogen atmo~phsre at 5 0;i psi for ~ hours. The ¢atalyst was filtered a~d the f iltrate conce~ltrated in vacuo . The residue wa~ dis-tilled at 45-50C. /0 . ~ torr to give 15 . 4 g. (9096 yield) of the desired intermediateq ~::
"
A2. cyclohexylacetyl chloride To 100 ml. of metha~ol ~s~taining lS.~ g. of ethyl cyclohexylacetate was added 15.2 g. of pot~ssium hydroxide and the 301ution refluxed for 3 hours. The ::~
methanol was removed ~n vacuo and the residue trea~ed with ~ater. The ~olution ~as extracted with diethyl ether and then a~idified with 10~ hydrochloric a~id~ ;
The acidified ~olution wa~ e~tracted ~ith fresh ether and ~he organic phase ~eparated and ~ashed with ~ater . . . , - :
3~ii7~
59 ~:
and a brine solution. Removal o~ the ~olvent after drying gave a liquid residue.
The residue was dissolved in 60 ~l. o~ ~ethylene chloride was treated with 18 ml. o~ oxalyl chloride.
After ~tirring at room temperature ~or 4 ~our~ the reac~ion mixture was concentrated und~r vacuum and the residue distilled~ 45/50C.t0.4 torr, 12.5 g~ ~86 yield).
PREPARATION B
Following the general prooedure of Pr~paration A
and starting with triethyl phosphonoacetal ~nd the appropriate aldehyde or ketone the following ~cid chlorides w~re prepared~
IS ~lCO~l l Z.P.-C./torr ~C~13C~2C~2i 2CEICEI2 ..
tC~3CH2)2C~c~2 22-~5/0.5 :
1~3 C~3(C~2)3C~C ~ 23-30~0.5 C~ -C~3~C~2)2~C~ - 22-25J0 ~R,S~
3a (c~3)2c~(c~2)3 Z4-3110.7 '~.' . .
,~ . . . ~
.; .
i7~3~
Rl ~ L~ ;
f~3C~2 .,, C~13 (~ ~2 ) 3CHCH~ 3~-37/ 0 ~ 5 ' f~3 :
CH3 (CH2) 4C9CH 45~47/O.li ,S~ :
25-30/O.S
~CH3C~2) 2CE~(C~2) 2 32 36/0.~ ~
Cl~ I .:
1 3 ,:
CH3 (C~2~ ;! tHR(S~ 2) 2 30-3~/.06 :.
I 3CH2 .'.
C~3 (C~2) 4 (RCS~ 63-65/ .35 CH3 (C92) 5 ~RCS j~ 89~215 :' f~3 (C~332C~(C~2),~C~C~2 46~;50/0~5 7~
-61~
Rl ~
CH ' "
1C~3)2C~C~2C~C~ - 30-34~.5 :
~R,S~ ~
., 3IH2 ..
(R,S~ 31-35/0.7 :;'i PREP~RATION ~
5~11~= .
Cl. 3-hydroxy-4-meth~ pentene To 90 ml. of l.OM vinyl magnesium bro~ide in tetrahydrofuran cooled to ~C. was added dropwise 6.3 -:
ml. of i~ob~tyraldehyde in 30 ml. of tetrahydrofuran and the mixture then allo~ed to warm to room temp~ratuse.
After 2 hours the reaction was added ~o a ~aturated ammonium ~hloride solution and extracted with ether.
The ether extracts were combined, ~ashed ~ith a .
sa~urated ammo~ium chloride solution~ a saturated sodium bicarbonate solution and a brine solution, and dried over magnesium sulfate. The solvent was removsd i~
vacuo to giv~ 6.0 g. of the desired product.
;.
:
' ' ',.
i~ ~ h e p ~eh o ~
'3~ C2. 5-~ethyl-4-he~n~e_acid ethyl ester A mixture of 18.2 g. of 3-h~dro ~ 4- ~thyl~l-pentene, 200 ml. of triethyl ~.r~e~EE~ and 500 ~1. of ~-toluenesulfonic acid ~as tre,ated with 400 ~l. oP
toluene and heated to reflux over 4A ~olecular ~ieve~
for 24 hours. The solvent was removed in cuo a~d the residue distilled~ The fractilon di tilling at ~5-64C./ ,~
0.5 torr ga~e 7OS g. of the desired pro~uct.
C3. 6-methylheptanoic acid ethyl ester To 7.5 g. of 6-~ethyl-4- ~ cld ethyl ester in 75 ml. of methanol w~s adde~ 700 ~g. of 10~ palladium hydroxide on carbon and the mixture shak~n in a hydrogen atmosphere at S0 psi for 1.5 hours. The cataly~t ~as filtered and the solvent removed under vacuum to glve 5.7 g. of the desired productO
C4. 6-meth~lhe~tanoyl chloride ~ ollowing the procedure of P~epa~atio~ A2, 5~7 g.
of 6-methylheptanoic acid ethyl ester gav~ 2~0 g. of the desired product, b.p. 30~34C./0.5 torr.
:
' ,',~.
I;
7~
PP P~TI~ D
~ y~oyl chloride Dl. 2-methylheetanoic acid To a cold (0C~l ~olution of 100 ~1~ o~ dry S tetrahydrofuran containing 11.8 ~1. o~ dry dii~opropyl a~ine and 55 ~1. of 1.6~ n butyl lithi~m was added 5.4 ml. of n~heptanoic acid and the mixture allow to stir at room temperature for one hour. The resulting solution was cooled to O~C. and 7.2 ml. of methyl iodide was added. The reaction was stirred at roo~ temperature under nitrogen for 105 hour~ 9 and wa~ then poured into 10% hydrochloric acid and extracted with diethyl ether (3 x 100 ml.). The extracts were co~bined, wa~hed with 10~ hydrochloric acid, water, 20~ ~odium bi~ulfite and a brine solution and dried over msgne~ium ~ul~ate. The solvent was removed in acuo a~d the residue, 5.61 g..
dissolved in ~ethanol containing 5.1- g. of potassium hydroxide~ After stirri~g overnight the mehtanol was ~0 removed and tbe residue dissolv~d in 150 ml. of water.
The aqueous layer was wa~ed with eth~r (2 x 100 ml~3 and acidified ~ith 10~ hydrochloric acid. ~he proauc was extracted with ether, washe~ with a 20~ sodium bisulfite solution and brine and dried over magnesium 23 sulf~te. Removal of th~ ether ga~e 5.0 g. of the product as a y~llow liquid.
D2. 2~ hvlhe~e-~ovl ~hio~id~
~ mploying 5 g. of 2-methylbeptanoic acid and 7 . 6 ml. of oxalyl chloride and usi~g the procedure of Preparation A2, 3.3 g. of the d~ired product was obtained, b.p. 32-34 ~C./0.6 to~xO
'7~
PREPARATION E
3-tS?~ L!~oe~lee~ a ~e`l ~9~b~olE~z~ ~on~ e ~VI ~e5f ,~ ~ re r To a 5 lo four ~eck~d fla~sk ~itted with a~ r~
and p~ electrode wa6 added 2.5 1. og ~01~ pota~ium acid phosphate buffer p~ 7.0 followed by 150 mg. of pig liver esterase and 150 9. of dimethyl 3-methylgluta~ate. The p~ of the mixture ~as maintaineaa at about 6.85 by periodic addition o a 10~ potassium carbonate ~olution.
After 2.5 hours the reaction was acidified ~ith 10%
hydr~chloxic acid to p~ 2.0 and the product e~tracted with diethyl ether. The extracts were so~bi~ed, dried over magnesium sulfate and concentrated in vacuo to give 1~ 114 g. of the desired product, lalphalD ~ -1.48 ~C~30 C=0.086 g/ml)-E2. met~l 3-(R)-m~Y¦C~ bo~
To 114 g. of 3-~R)-~ethylgl~taric acid ~ono methyl ester in 715 ml. of dry tetrahydrofuran cooled to 0CO
~as added slowly 391 ml~ of 2M solution sf bor~ne dimethylsulfide in tetrahydrofuran. After the ~ddition was complete the reaction mixture wa~ ~ti~r~d o~ernigh~
at room temperature. The reaction wa~ cooled and 50 ml.
of water slowly added. The reaction was extracted ~3 x 100 ml.~ with ekher and the extracts co~bined, washed with water, ~ ~aturated sodium bica~bo~ate 801ution and a brine solution, and dried over magnesium ~ulfate.
~emoval of the solvent gave 37 g. of the de~ired product. l, 3~ :
~ ,'~ 7~ ~ :
:, E3. methyl 3-~R~-methyl-5-(t-butyldimethyl-To a solution of 37 q. ~.253 ~ o~ ~ethyl 3-(R)-~ethyl-5-hydroxyp~nta~o~te and 37 g. l.5~3 ~) Of ~idazole in 500 ~1. of dLmethylformamide was added 37 g. ~.249 m) of t-butyldimethyl~ilyl chloride a~d the reaction stirred at room temperature for 2 hour~. The .:
reaction mixture was poured i.nto water ~nd ~xtracted ~4 x 10~ ml.) ~ith ether. The co~bined extra~ts were 10 washed with 10% hydrochloric a~id, a saturated sodiu~ ;
bicarbonate 801ution, water ~nd a bri~e solutisn, and dried over magne~ium sulfate. Re~oval of the solvent gave 12I.88 g. of crude product which, on distillation, gave 107.12 g. of pure product, b.p. 80 81C./0.4 torr.
E4. 3-(S)-methyl-5-(t-butvldimethyl~ilyloX~ Pentanol To 8.5 g. (.224 m) of lithiu~ aluminum hydride in 250 ml. of die~hyl ether under nit~oge~ was add~d 53.5 g. (.206 m) o methyl 3- ~R~ -methyl-5- (t-butyl~methyl- :
silyloxy)pen~anoate in 1~5 ml. o~ etherO The reactio~
was stirred for one hour at 0C. and wa~ then treated dropwis~ with 8.4 g. of water, 8.~ ml. of a 15% 80dium hydroxida solu~ion and 25.2 ml. of water. The solids were filtered and -he organic phase separated and washed 2S with water, 2.59~i hydrochloric acid arld a l:rine ~olution.
Th2 organic phase ~as dried over ~a~nesi~m ~ulfate and concentrated in vacuo to give 46 q. oiE produ~:t.
~:5. =~_~
~o 56.3 g. of oxalyl chloride in 3D0 ml. of dry methylene chloride cooled to -60C. 2nd under a ~i~r~gen atmosphere was added dropwi~e 74~81 y. of d~methyl~
sulfoxide in 100 ml~ o~ dry ~ethylene chlorideO After 15 minutes 92.0 g. oP 3-(S)-methyl 5 (t-butyldimethyl-silyloxy)-1-penta~ol in 250 ~1. of th~ same 301~e~t was i I., .
.:
-66- :
added dropwise. After 30 minutes 206.1 g. of triethyl-amine was added to -60C. followed by the r~moval of the cooling ~ath. The reaction was stirred at room t~mpera- :
ture for l.S hours, and was then poured into water a~d extracted with methylene chloride. The extract~ were washed with 2.5~ hydrochlori~, a 8aturat~d ~oaium bicarbonate solution, water and a b~ine 801ution, dnd then dxied over magnesium sulfate. The ~olve~t wa~
removed and the re idue dissol~ed in ether and rewashed and dried as before. Removal o~ the ether ga~e 90.9 g.
of the desired p~oduct.
E6. 5-(S)-methyl-7-(t-butyldimethylsilyloxy?-2-heptene To a slurry of 80 g. (.2155 m) of triphenyl-ethyl phosphonium ~romide in 800 ml. of dry tetrahydrofuran cooled to 0C. was added 165.7 ml. of 1,3~ ~olution of n-butyl lithium (.215S ~) in the ~ame ~olvent. After 2 hours, 45 gO ( r 196 m) 3-~R)-methyl-5-(t-butyldimethyl-silyloxy)-1-pentanal in 200 ml. of d~y t~trahydrofuran was added dropwise to the rea~tion mi~ture. The reaction was allowed to stir 2 hours at room temp~ratu~e and was then poured i~to wa~er and e~tracted with ether.
The combined extracts were washed with water and a brine solution and dried over magnesium ~ulfate. Removal of the solvent in vacuo gave a ye}low oil which, on di~til-lation gaYe 37.4 g. of product, b~p~ 74-79C./.2~
torr. -~7. 3-~S?-methyl-l-heptanol ~-To a solution of 74.8 g. of 5-~S)-methyl-7-(t-butyldimethylsilyloxy)-2-hepte~e in 500 ~1. of methanol was added 7.5 g. of 10% palladium hydroxide on car~o~ :
and the mixture sha~e~ in a hydrogen atmosphere for 1.5 hours at 50 psi. The catalyst wa~ filtexed a~d the solvent removsd under vacuum, 30 g.
.
E8. 3-(S)-methylhepta~oic ~ci~
To 10 g. of 3-(S)-methyl-1-heptanol in 175 ~1. of acetone was added over 45 ~inutes 90 ~1. of Jones reagent dropwise at 15-20C. Aft~r 15 ~inu~s, 15 ml.
of isopropanol was added ~nd stirri~g conti~ued ~or 30 minutes. The reaction was poured into water and the product extracted with ether. The extracts were - combined, washed with water, a sodium bi~ulfit~ ~olution and a brine solution and dried over mag~e9ium ~ulfate.
Removal of the solve~t gave 10 g. o~ the produ~t as a liquid, b.p. 84-88C./.~ torr, ~alphal~ 4.46 ~C~3O~
C=0.105 g/ml.).
E9. 3-~S~-methylheetanoyl_chloride Following the proçedure of Prepar~tion A2, 5.0 g. , of 3-~S)~me~hylheptanoic acid and ~.5 ~1. of oxalyl chloride gave 2.9 g. of the desired acid chloride, b.p.
29-32C./.25 torr~ ::
DD ' :
''' ;'.
:-.
Claims (32)
1. A compound of the formula:
(1) (wherein R1 is selected from the group consisting of cycloalkyl having four to seven carbon atoms, alkyl having two to ten carbon atoms and cycloalkylmethyl having from six to eight carbon atoms;
R2 is hydrogen;
R3 is a D-amino acid residue of the formula:
-NH?H(CH2)nCO2R5 (2) [wherein X is selected from the group consisting of hydrogen, alkyl having one to two carbon atoms and hydroxymethyl, and n is an integer of 0 to 4; and R4 and R5 are each selected from the group consisting of hydrogen, alkyl having one to six carbon atoms, cycloalkylmethyl having six to eight carbon atoms and benzyl]), or a pharmaceutically acceptable base salt thereof.
(1) (wherein R1 is selected from the group consisting of cycloalkyl having four to seven carbon atoms, alkyl having two to ten carbon atoms and cycloalkylmethyl having from six to eight carbon atoms;
R2 is hydrogen;
R3 is a D-amino acid residue of the formula:
-NH?H(CH2)nCO2R5 (2) [wherein X is selected from the group consisting of hydrogen, alkyl having one to two carbon atoms and hydroxymethyl, and n is an integer of 0 to 4; and R4 and R5 are each selected from the group consisting of hydrogen, alkyl having one to six carbon atoms, cycloalkylmethyl having six to eight carbon atoms and benzyl]), or a pharmaceutically acceptable base salt thereof.
2. The compound or salt of claim 1, wherein R1 is alkyl having five to eight carbon atoms, R3 is an amino acid residue of the formula:
-NH?H(CH2)nCO2R5 (wherein X is selected from the group consisting of hydrogen and alkyl having one to two carbon atoms, n is an integer of 0 to 4 and R5 is selected from the group consisting of hydrogen, alkyl having one to six carbon atoms, cycloalkylmethyl having six to eight carbon atoms and benzyl, and R4 is hydrogen).
-NH?H(CH2)nCO2R5 (wherein X is selected from the group consisting of hydrogen and alkyl having one to two carbon atoms, n is an integer of 0 to 4 and R5 is selected from the group consisting of hydrogen, alkyl having one to six carbon atoms, cycloalkylmethyl having six to eight carbon atoms and benzyl, and R4 is hydrogen).
3. The compound or salt of claim 2, wherein n is 0 and R5 is selected from the group consisting of hydrogen, alkyl having one to six carbon atoms and cyclohexylmethyl.
4. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-butyl, X is methyl and R5 is hydrogen.
5. The compound or salt of claim 3, wherein R1 is (S,R) 3-heptyl, X is methyl and R5 is hydrogen.
6. The compound or salt of claim 3, wherein R1 is (S,R) 2-heptyl, X is methyl and R5 is hydrogen.
7. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-pentyl, X is methyl and R5 is hydrogen.
8. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-pentyl, X is methyl and R5 is n-butyl.
9. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-pentyl, X is methyl and R5 is i-butyl.
10. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-pentyl, X is methyl and R5 is cyclohexylmethyl.
11. The compound or salt of claim 3, wherein R1 is (S) 2-methyl-1-hexyl, X is methyl and R5 is hydrogen.
12. The compound or salt of claim 3, wherein R1 is (S) 2-methyl-1-hexyl, X is methyl and R5 is n-butyl.
13. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-hexyl, X is methyl and R5 is hydrogen.
14. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-hexyl, X is methyl ancd R5 is n-butyl.
15. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-hexyl, X is methyl and R5 is 1-butyl.
16. The compound or salt of claim 3, wherein R1 is (S,R) 2-ethyl-1-hexyl, X is methyl and R5 is cyclohexylmethyl.
17. The compound or salt of claim 3, wherein R1 is (S) 2-ethyl-1-hexyl, X is methyl and R5 is hydrogen.
18. The compound or salt of claim 3, wherein R1 is (S) 2-ethyl-1-hexyl, X is methyl and R5 is n-butyl.
19. The compound or salt of claim 3, wherein R1 is (S) 2-ethyl-1-hexyl, X is methyl and R5 is cyclohexylmethyl.
20. The compound or salt of claim 3, wherein R1 is (S,R) 2-methyl-1-hexyl, X is methyl and R5 is hydrogen.
21. The compound or salt of claim 3, wherein R1 is (S,R) 2-methyl-1-hexyl, X is methyl and R5 is n-butyl.
22. The compound or salt of claim 3, wherein R1 is (S,R) 2-methyl-1-hexyl, X is methyl and R5 is i-butyl.
23. The compound or salt of claim 3, wherein R1 is (S,R) 2-methyl-1-hexyl, X is methyl and R5 is cyclohexylmethyl.
24 The compound or salt of claim 3, wherein R1 is (S,R) 2-methyl-1-pentyl, X is methyl and R5 is hydrogen.
25. The compound or salt of claim 3, wherein R1 is (S,R) 1-hexyl, X is methyl and R5 is hydrogen.
26. The compound or salt of claim 2, wherein R1 is 1-hexyl, X is hydrogen, n is 3 and R5 is hydrogen.
27. The compound or salt of claim 1, wherein R1 is cycloalkyl having four to seven carbon atoms, R3 is an amino acid residue of the formula:
-NH?H(CH2)nCO2R5 (wherein n is 0 and X is alkyl having one to two carbon atoms, and R4 and R5 are each hydrogen).
-NH?H(CH2)nCO2R5 (wherein n is 0 and X is alkyl having one to two carbon atoms, and R4 and R5 are each hydrogen).
28. The compound or salt of claim 27, wherein R1 is cyclohexyl and X is methyl.
29. The compound or salt of claim 1, wherein R1 is alkyl having five to eight carbon atoms, R3 is an amino acid residue of the formula:
-NH?H(CH2)nCO2R5 (wherein X is selected from the group consisting of hydrogen and alkyl having one to two carbon atoms, n is an integer of 0 to 4 and R5 is hydrogen), and R4 is selected from the group consisting of alkyl having one to six carbon atoms, cycloalkylmethyl having six to eight carbon atoms and benzyl.
-NH?H(CH2)nCO2R5 (wherein X is selected from the group consisting of hydrogen and alkyl having one to two carbon atoms, n is an integer of 0 to 4 and R5 is hydrogen), and R4 is selected from the group consisting of alkyl having one to six carbon atoms, cycloalkylmethyl having six to eight carbon atoms and benzyl.
30. A pharmaceutical composition in unit dosage form comprising a pharmaceutically acceptable carrier and an antiinfective or immunostimulant effective amount of the compound or salt according to claim 1, 2 or 3.
31. A process for preparing the compound or salt as defined in claim 1, which process comprises:
[A] acylating the amino group in a peptide of the formula:
(3) (wherein R2, R3 and R4 are as defined in claim 1 except that if they interfere with the reaction, they may be blocked by a blocking group), with a carboxylic acid of the formula:
R1COOH (4) (wherein R1 is as defined in claim 1) or an activated derivative thereof, followed by, if necessary, the removal of the blocking group, or [B] amidating the carboxyl group or the activated form thereof in a compound of the formula:
(5) (wherein R1, R2 and R4 are as defined in claim 1 except that if they interfere with the reaction they may be blocked by a blocking group, and the carboxyl group attached to the group -CHR2- may be in an activated form), with an amino compound of the formula:
(6) (wherein X, n and R5 are as defined in claim 1 except that if they interfere with the reaction, they may be blocked by a blocking group), followed by, if necessary, the removal of the blocking agent, thereby producing a compound (1) , or [C] amidating a D-glutamic acid derivative of the formula:
(7) (wherein R1 and R4 are as defined in claim 1, except that if they interfere with the reaction, they may be blocked by a blocking group), or an activated derivative thereof, with an amino compound of the formula:
(8) (wherein R2 and R3 are as defined in claim 1, except that if they interfere with the reaction, they may be blocked by a blocking group), followed by, if necessary, the removal of the blocking group, and [D] if required, converting a compound (1) produced by any one of processes [A] through [C] into a pharmaceutically acceptable base salt thereof.
[A] acylating the amino group in a peptide of the formula:
(3) (wherein R2, R3 and R4 are as defined in claim 1 except that if they interfere with the reaction, they may be blocked by a blocking group), with a carboxylic acid of the formula:
R1COOH (4) (wherein R1 is as defined in claim 1) or an activated derivative thereof, followed by, if necessary, the removal of the blocking group, or [B] amidating the carboxyl group or the activated form thereof in a compound of the formula:
(5) (wherein R1, R2 and R4 are as defined in claim 1 except that if they interfere with the reaction they may be blocked by a blocking group, and the carboxyl group attached to the group -CHR2- may be in an activated form), with an amino compound of the formula:
(6) (wherein X, n and R5 are as defined in claim 1 except that if they interfere with the reaction, they may be blocked by a blocking group), followed by, if necessary, the removal of the blocking agent, thereby producing a compound (1) , or [C] amidating a D-glutamic acid derivative of the formula:
(7) (wherein R1 and R4 are as defined in claim 1, except that if they interfere with the reaction, they may be blocked by a blocking group), or an activated derivative thereof, with an amino compound of the formula:
(8) (wherein R2 and R3 are as defined in claim 1, except that if they interfere with the reaction, they may be blocked by a blocking group), followed by, if necessary, the removal of the blocking group, and [D] if required, converting a compound (1) produced by any one of processes [A] through [C] into a pharmaceutically acceptable base salt thereof.
32. A use of the compound or salt according to claim 1, 2 or 3, for treating an infection in a human suffering therefrom.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
USPCT/US85/02351 | 1985-11-25 | ||
US8502351 | 1985-11-25 | ||
US06/900,934 US4767743A (en) | 1986-08-27 | 1986-08-27 | Peptide immunostimulants |
US900,934 | 1986-08-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1295784C true CA1295784C (en) | 1992-02-11 |
Family
ID=26772151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000523519A Expired - Fee Related CA1295784C (en) | 1985-11-25 | 1986-11-21 | Peptide immunostimulants |
Country Status (12)
Country | Link |
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KR (1) | KR900004648B1 (en) |
CN (1) | CN1017436B (en) |
AU (1) | AU579501B2 (en) |
CA (1) | CA1295784C (en) |
DK (1) | DK170345B1 (en) |
FI (1) | FI86858C (en) |
NO (1) | NO170422C (en) |
PH (1) | PH22258A (en) |
PL (2) | PL150129B1 (en) |
PT (1) | PT83796B (en) |
SU (1) | SU1560058A3 (en) |
YU (1) | YU46183B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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US4851388A (en) * | 1986-01-23 | 1989-07-25 | Pfizer Inc. | Heptanoyl-glu-asp-ala-amino acid immunostimulants |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US4565653A (en) * | 1984-03-30 | 1986-01-21 | Pfizer Inc. | Acyltripeptide immunostimulants |
WO1988001612A1 (en) * | 1986-08-27 | 1988-03-10 | Pfizer Inc. | Processes and intermediates for n-(s-3-alkyl-heptanoyl)-d-gamma-glutamyl-glycyl-d-alanine |
-
1986
- 1986-11-21 CA CA000523519A patent/CA1295784C/en not_active Expired - Fee Related
- 1986-11-24 PL PL1986268313A patent/PL150129B1/en unknown
- 1986-11-24 PH PH34518A patent/PH22258A/en unknown
- 1986-11-24 DK DK561986A patent/DK170345B1/en not_active IP Right Cessation
- 1986-11-24 PT PT83796A patent/PT83796B/en not_active IP Right Cessation
- 1986-11-24 NO NO864689A patent/NO170422C/en unknown
- 1986-11-24 AU AU65618/86A patent/AU579501B2/en not_active Ceased
- 1986-11-24 CN CN86107931A patent/CN1017436B/en not_active Expired
- 1986-11-24 YU YU200586A patent/YU46183B/en unknown
- 1986-11-24 KR KR1019860009903A patent/KR900004648B1/en not_active IP Right Cessation
- 1986-11-24 PL PL1986262563A patent/PL150055B1/en unknown
- 1986-11-24 FI FI864772A patent/FI86858C/en not_active IP Right Cessation
-
1987
- 1987-11-17 SU SU874203671A patent/SU1560058A3/en active
Also Published As
Publication number | Publication date |
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PL262563A1 (en) | 1988-04-28 |
PT83796B (en) | 1989-06-30 |
FI864772A (en) | 1987-05-26 |
DK170345B1 (en) | 1995-08-07 |
CN86107931A (en) | 1987-07-01 |
FI86858B (en) | 1992-07-15 |
DK561986D0 (en) | 1986-11-24 |
AU6561886A (en) | 1987-05-28 |
PH22258A (en) | 1988-07-01 |
PL150129B1 (en) | 1990-04-30 |
YU46183B (en) | 1993-05-28 |
FI864772A0 (en) | 1986-11-24 |
KR900004648B1 (en) | 1990-07-02 |
NO170422C (en) | 1992-10-14 |
YU200586A (en) | 1988-06-30 |
PT83796A (en) | 1986-12-01 |
CN1017436B (en) | 1992-07-15 |
NO864689D0 (en) | 1986-11-24 |
FI86858C (en) | 1992-10-26 |
DK561986A (en) | 1987-08-12 |
KR870005012A (en) | 1987-06-04 |
SU1560058A3 (en) | 1990-04-23 |
AU579501B2 (en) | 1988-11-24 |
PL150055B1 (en) | 1990-04-30 |
PL268313A1 (en) | 1988-07-07 |
NO170422B (en) | 1992-07-06 |
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