BRPI0616533A2 - polinucleotìdeo isolado, fragmento de ácido nucléico isolado, construções de dna recombinante, plantas, sementes, células vegetais, tecidos vegetais, método de isolamento de fragmentos de ácidos nucléico, método de mapeamento de variações genéticas, método de cultivo molecular, plantas de milho, métodos de alteração do transporte de nitrogênio das plantas e variantes de hat de plantas alteradas - Google Patents

Abstract

POLINUCLEOTìDEO ISOLADO, FRAGMENTO DE áCIDO NUCLéICO ISOLADO, CONSTRUçõES DE DNA RECOMBINANTE, PLANTAS, SEMENTES, CéLULAS VEGETAIS, TECIDOS VEGETAIS, MéTODO DE ISOLAMENTO DE FRAGMENTOS DE ACIDO NUCLéICO, MéTODO DE MAPEAMENTO DE VARIAçõES GENéTICAS, MéTODO DE CULTIVO MOLECULAR, PLANTAS DE MILHO, MéTODOS DE ALTERAçãO DO TRANSPORTE DE NITROGêNIO DAS PLANTAS E VARIANTES DE HAT DE PLANTAS ALTERADAS. A presente invenção refere-se a fragmentos de ácido nucléico isolado que codificam componentes de transporte de nitrato com alta afinidade. A presente invenção também se refere à elaboração de construções de DNA recombinante que codificam, no todo ou em parte, componentes de transporte de nitrato, em orientação com ou sem sentido, em que a expressão da construção de DNA recombinante pode alterar os níveis dos componentes de transporte de nitrato em célula hospedeira transformada.

Description

"POLINUCLEOTÍDEO ISOLADO, FRAGMENTO DE ÁCIDO NUCLÉICOISOLADO, CONSTRUÇÕES DE DNA RECOMBINANTE, PLANTAS,SEMENTES, CÉLULAS VEGETAIS, TECIDOS VEGETAIS, MÉTODO DEISOLAMENTO DE FRAGMENTOS DE ÁCIDO NUCLÉICO, MÉTODO DEMAPEAMENTO DE VARIAÇÕES GENÉTICAS, MÉTODO DE CULTIVOMOLECULAR, PLANTAS DE MILHO, MÉTODOS DE ALTERAÇÃO DOTRANSPORTE DE NITROGÊNIO DAS PLANTAS E VARIANTES DE HAT DEPLANTAS ALTERADAS"

Campo da Invenção

A presente invenção encontra-se no campo de biologia moleculardas plantas. Mais especificamente, a presente invenção refere-se a fragmentosde ácido nucléico que codificam transportadores de nitrato com alta afinidadeem plantas e sementes.

Antecedentes da Invenção

As plantas superiores são organismos autotróficos que podemsintetizar todos os seus componentes moleculares a partir de nutrientesinorgânicos obtidos do ambiente local. Nitrogênio é elemento fundamental emmuitos compostos presentes em células de plantas. Ele é encontrado nosfosfatos de nucleosídeos e aminoácidos que formam os blocos de construçãode ácidos nucléicos e proteínas, respectivamente. A disponibilidade denitrogênio para plantas de safras é fator limitador importante na produçãoagrícola e a importância de nitrogênio é demonstrada pelo fato de que somenteoxigênio, carbono e hidrogênio são mais abundantes em células de plantassuperiores. O nitrogênio presente na forma de amônia ou nitrato é facilmenteabsorvido e assimilado por plantas superiores.

Nitrato é a principal fonte de nitrogênio que é disponível paraplantas superiores sob condições normais de campo. Desta forma, o processode assimilação de nitrato é o principal ponto de entrada de nitrogênioinorgânico para compostos orgânicos (Hewitt et al (1976), Plant Biochemistry,págs. 633-6812, Bonner e Varner1 eds., Academic Press, Nova Iorque).Embora algumas plantas utilizem amônia diretamente, sob certas condições,nitrato é geralmente a principal forma de nitrogênio disponível para as plantas.

A absorção de nitrato por células de raízes é a primeira etapa doprocesso de assimilação de nitrato em plantas superiores (Orsel et al (2002),Plant Physiology 129: 886-896). As plantas desenvolveram dois sistemas deabsorção diferentes para adequar-se à disponibilidade variável de nitrato emsolos cultivados. O sistema de transporte de nitrato com baixa afinidade épreferencialmente utilizado quando a concentração de nitrato externo é alta,enquanto o sistema de transporte com alta afinidade (HATS) tem lugar emconcentrações externas muito baixas.

Em plantas superiores, foram identificadas duas famíliasgenéticas: as famílias NRT1 e NRT2 envolvidas no sistema de transporte combaixa afinidade e HATs, respectivamente. A complexidade de transporte denitrato e nitrito é aumentada pela regulagem fina que ocorre no nível detranscrição: sistemas com baixa e alta afinidade possuem componentesconstitutivos e indutíveis que são claramente distintos. Além disso, algunsmembros dos transportadores de nitrato necessitam de segundo produtogenético, polipeptídeo do tipo NAR2, para funcionar (Tong et al (2005), ThePlant JournaIAI: 442-450).

As seqüências de nucleotídeos de acordo com o presente pedidoe os métodos de sua utilização podem aumentar a eficiência com a qualnitrogênio pode ser utilizado.

Descrição Resumida da Invenção

A presente invenção inclui polinucleotídeos isolados quecodificam polipeptídeo necessário para transporte de nitrato com alta afinidade,em que a seqüência de aminoácidos do polipeptídeo e a seqüência deaminoácidos de SEQ ID N0 36 ou 49 possuem pelo menos 80%, 85%, 90%,95%, 99% ou 100% de identidade para (b) o complemento da seqüência denucleotídeos, em que o complemento e a seqüência de nucleotídeos contêm omesmo número de nucleotídeos e são 100% complementares. O polipeptídeocompreende preferencialmente a seqüência de aminoácidos de SEQ ID N0 36ou 49. A seqüência de nucleotídeos compreende preferencialmente aseqüência de nucleotídeos de SEQ ID N0 35 ou 48.

Em primeira realização, a presente invenção inclui polinucleotídeoisolado que compreende: (a) seqüência de nucleotídeos que codificapolipeptídeo necessário para transporte de nitrato com alta afinidade, em que opolipeptídeo contém seqüência de aminoácidos com pelo menos 80%, 85%,90%, 95%, 99% ou 100% de identidade de seqüências com base no método dealinhamento Clustal V em comparação com polipeptídeo SEQ ID N0 36 ou 49.

(b) complemento da seqüência de nucleotídeos, em que ocomplemento e a seqüência de nucleotídeos contêm a mesma quantidade denucleotídeos e são 100% complementares.

Em segunda realização, a presente invenção refere-se a essaseqüência de nucleotídeos isolados ou seu complemento que compreende pelomenos dois motivos que correspondem substancialmente a qualquer dasseqüências de aminoácidos descritas em SEQ ID N0 50, 51 ou 52, em que omencionado motivo é substancialmente subseqüência conservada. Exemplosdesses motivos, entre outros que podem ser identificados, são exibidos emSEQ ID N0 50, 51 ou 52. Também é de interesse o uso desse fragmento ou suaparte em inibição sem sentido ou co-supressão em planta transformada.

Em terceira realização, a presente invenção refere-se a seucomplemento de fragmento de nucleotídeo isolado, em que o fragmento ouparte dele é útil em inibição sem sentido ou co-supressão de proteína quealtere o transporte de nitrato em planta transformada.Em quarta realização, a presente invenção refere-se a fragmentode ácido nucléico isolado que compreende promotor em que o mencionadopromotor consiste essencialmente da seqüência de nucleotídeos descrita emSEQ ID N0 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73, 74, 89 ou 90, ou omencionado promotor consiste essencialmente de fragmento ou subfragmentoque é substancialmente similar e funcionalmente equivalente à seqüência denucleotídeos descrita em SEQ ID N0 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71,72, 73, 74, 89 ou 90.

Em quinta realização, a presente invenção refere-se aconstruções de DNA recombinantes que compreendem qualquer fragmento deácido nucléico acima ou seu complemento, ou parte de qualquer deles, ligadooperativamente a pelo menos uma seqüência reguladora. Também são deinteresse plantas que compreendem essas construções de DNA recombinantesno seu genoma, tecido de plantas ou células obtidas dessas plantas esementes obtidas dessas plantas.

Em sexta realização, a presente invenção refere-se a método dealteração do transporte de nitrato em plantas, que compreende:

a. transformação de planta com construção de DNArecombinante que compreende:

i. primeira construção de DNA recombinante quecompreende polinucleotídeo isolado que codifica polipeptídeo HAT, ligadooperativamente a pelo menos uma seqüência reguladora; e

ii. pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado que codifica polipeptídeoNAR, ligado operativamente a pelo menos uma seqüência reguladora;

b. cultivo da planta transformada de a sob condiçõesapropriadas para a expressão das construções de DNA recombinante; eseleção das plantas transformadas que possuem transporte de nitrato alterado.Plantas de milho que compreendem essas construções recombinantes tambémsão parte da presente invenção.

Em sétima realização, a presente invenção refere-se a método deisolamento de fragmentos de ácidos nucléicos que codificam polipeptídeosassociados à alteração do transporte de nitrato que compreende:

a. comparação de SEQ ID N0 36, 49, 55 ou 58 com outrasseqüências de polipeptídeos associadas à alteração do transporte de nitrato daplanta;

b. identificação da(s) seqüência(s) conservada(s) de quatroou mais aminoácidos obtidos na etapa a;

c. elaboração de oligômero(s) ou sonda(s) de nucleotídeosespecíficos de região com base nas seqüências conservadas identificadas naetapa b; e

d. uso do(s) oligômero(s) ou sonda(s) de nucleotídeos daetapa c para isolar seqüências associadas à alteração do transporte de nitratopor meio de protocolos dependentes de seqüências.

Em oitava realização, a presente invenção também se refere amétodo de mapeamento de variações genéticas referentes à alteração dotransporte de nitrato das plantas:

a. cruzamento de duas variedades de plantas; e

b. avaliação de variações genéticas com relação a:

i. seqüência de ácidos nucléicos selecionada a partirdo grupo que consiste de SEQ ID N0 35, 48, 54 e 57; ou

ii. seqüência de ácidos nucléicos que codificapolipeptídeo selecionado a partir do grupo que consiste de SEQ ID N0 36, 49,55 e 58;

em plantas progenitoras resultantes do cruzamento da etapa (a), emque a avaliação é realizada utilizando método selecionado a partir do grupo queconsiste de: análise de RFLP, análise de SNP e análise com base em PCR.

Em nona realização, a presente invenção refere-se a método decultivo molecular para obter transporte de nitrato de plantas alterado, quecompreende:

a. cruzamento de duas variedades de plantas; e

b. avaliação de variações genéticas com relação a:

i. seqüência de ácidos nucléicos selecionada a partirdo grupo que consiste de SEQ ID N0 35, 48, 54 e 57; ou

ii. seqüência de ácidos nucléicos que codificapolipeptídeo selecionado a partir do grupo que consiste de SEQ ID N0 36, 49,55 e 58;

em plantas progenitoras resultantes do cruzamento da etapa a,em que a avaliação é realizada utilizando método selecionado a partir do grupo queconsiste de: análise de RFLP, análise de SNP e análise com base em PCR.

Em décima realização, a presente invenção refere-se a método dealteração do nível de expressão de polipeptídeo transportador de nitrato comalta afinidade em célula hospedeira que compreende: (a) transformação decélula hospedeira com construção de DNA recombinante que compreende:

b. seqüência de nucleotídeos que codifica polipeptídeotransportador de nitrato com alta afinidade, em que o polipeptídeo contémseqüência de aminoácidos com pelo menos 80% de identidade de seqüências,com base no método de alinhamento Clustal V, em comparação com umadentre SEQ ID N0 36 ou 49, e o polipeptídeo altera o transporte de nitrato, seucomplemento ou pelo menos dois motivos que correspondem substancialmentea qualquer das seqüências de aminoácidos descritas em SEQ ID N0 50, 51 e52, em que o mencionado motivo é subseqüência substancialmenteconservada ligada operativamente a pelo menos uma seqüência reguladora; e

c. cultivo da célula hospedeira transformada sob condiçõesque são apropriadas para expressão da construção de DNA recombinante, emque a expressão da construção de DNA recombinante resulta na produção deníveis alterados do polipeptídeo necessário para transporte de nitrato na célulahospedeira transformada.

Em décima-primeira realização, a presente invenção refere-se aplanta de milho, que compreende primeira construção de DNA quecompreende polipeptídeo HAT isolado, ligado operativamente a pelo menosuma seqüência reguladora; e pelo menos uma construção de DNArecombinante adicional que compreende polinucleotídeo isolado, ligadooperativamente a pelo menos uma seqüência reguladora, que codificapolipeptídeo selecionado a partir do grupo que consiste de NAR2.

Realização adicional da presente invenção refere-se a método dealteração do transporte de nitrogênio das plantas, que compreende:

a. transformação de planta com construção de DNArecombinante que compreende:

i. primeira construção de DNA recombinante quecompreende polinucleotídeo isolado que codifica polipeptídeo HAT, ligadooperativamente a pelo menos uma seqüência reguladora; e

ii. pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado, ligado operativamente apelo menos uma seqüência reguladora, que codifica polipeptídeo selecionado apartir do grupo que consiste de NAR;

b. cultivo da planta transformada de a sob condiçõesapropriadas para a expressão da construção de DNA recombinante; e

c. seleção das plantas transformadas que possuem transportede nitrato alterado.

Realizações adicionais da presente invenção incluem variantesde HAT alteradas (shuffled HAT variants) com parâmetros cinéticosaprimorados, construções de DNA recombinante que compreendem asseqüências de nucleotídeos que codificam estas variantes e plantas ecélulas transformadas que compreendem no seu genoma estas construçõesde DNA recombinante. Também são incluídas na presente invenção plantasde milho que compreendem primeira construção de DNA recombinante quecompreende seqüência de nucleotídeos que codificam variante de HATalterada, ligada operativamente a pelo menos uma seqüência reguladora epelo menos uma construção de DNA recombinante adicional quecompreende polinucleotídeo isolado, ligada operativamente a pelo menosuma seqüência reguladora, que codifica polipeptídeo selecionado a partir dogrupo que consiste de NAR.

Ainda outra realização da presente invenção descreve método dealteração do transporte de nitrogênio das plantas, que compreende: a)transformação de planta com construção de DNA recombinante quecompreende primeira construção de DNA recombinante que compreendeseqüência de nucleotídeos que codifica variante de HAT alterada, ligadaoperativamente a pelo menos uma seqüência reguladora e pelo menos umaconstrução de DNA recombinante adicional que compreende polinucleotídeoisolado, ligada operativamente a pelo menos uma seqüência reguladora, quecodifica polipeptídeo selecionado a partir do grupo que consiste de NAR; e b)cultivo da planta transformada de (a) sob condições apropriadas para aexpressão da construção de DNA recombinante; e seleção das plantastransformadas que possuem transporte de nitrato alterado.

Depósitos Biológicos

Os plasmídeos a seguir foram depositados junto à Coleção Norte-Americana de Cultivos de Tipos (ATCC), 10801 University Boulevard,Manassas VA 20110-2209, e contêm as designações, números de acesso edatas de depósito a seguir:<table>table see original document page 10</column></row><table>

Breve Descrição das Listagens de Seqüências

A presente invenção pode ser mais completamente compreendidaa partir do relatório descritivo detalhado a seguir e das figuras e Listagens deSeqüências anexas, que fazem parte do presente pedido.

A Fig. 1 é esquema do vetor PHP 27621.

A Fig. 2 é esquema do vetor PHP 27660.

A Fig. 3 é esquema do vetor PHP 27860.

A Fig. 4 é esquema do vetor PHP 27280.

A Fig. 5 é esquema do vetor PHP 27281.

A Fig. 6 é esquema do vetor PHP 27282.

A Fig. 7 é esquema do vetor PHP 27283.

SEQ ID N0 1 é o primer frontal utilizado no Exemplo 3.

SEQ ID N0 2 é o primer reverso utilizado no Exemplo 3.

SEQ ID N0 3 é o primer T7 utilizado no Exemplo 3 para

seqüenciamento de extremidades de BAC confirmadoras.

SEQ ID N0 4 é o primer SP6 utilizado no Exemplo 3 paraseqüenciamento de extremidades de BAC confirmadoras.

SEQ ID N0 5 a 33 são os primers de seqüenciamento utilizados paracobrir a região sobre o clone de BAC bacc.pk139.d24 que contém o gene HAT4.

SEQ ID N0 34 representa os 3924 bp da seqüência genômica demilho que contém ORF (nucleotídeos 2015-3583 (Parada)) do gene quecodifica o transportador de nitrato com alta afinidade (HAT4) isolado do clonede BAC bacc.pk139.d24.

SEQ ID N0 35 é 1569 bp da seqüência de nucleotídeos de ORFde SEQ ID N0 34.

SEQ ID N0 36 é a seqüência de aminoácidos codificada pelosnucleotídeos 2015 a 3580 de SEQ ID N0 34.

SEQ ID N0 37 é 2014 bp, que se estende a partir dos nucleotídeos1 a 2014 do suposto promotor da seqüência genômica transportadora de nitratocom alta afinidade de milho exibida em SEQ ID N0 34.

SEQ ID N0 38 é 1014 bp, que se estende a partir dos nucleotídeos1001 a 2014 do suposto promotor da seqüência genômica transportadora denitrato com alta afinidade de milho exibida em SEQ ID N0 34.

SEQ ID N0 39 a 42 são os primers frontal e reverso utilizados noExemplo 4.

SEQ ID N0 43 é o primer T3 utilizado no Exemplo 4.

SEQ ID N0 44 é o primer T7 utilizado no Exemplo 4.

SEQ ID N0 45 representa 5812 bp da seqüência genômica demilho que contém ORF (nucleotídeos 2264 a 3450 e 5087 a 5357 (Parada)) dogene que codifica transportador de nitrato com alta afinidade (HAT7).

SEQ ID N0 46 é 2263 bp, que se estende a partir dos nucleotídeos1 a 2263 do suposto promotor da seqüência genômica transportadora de nitratocom alta afinidade de milho exibida em SEQ ID N0 45.

SEQ ID N0 47 é 1263 bp, que se estende a partir dos nucleotídeos1001 a 2263 do suposto promotor da seqüência genômica transportadora denitrato com alta afinidade de milho exibida em SEQ ID N0 45.

SEQ ID N0 48 é 1455 bp da seqüência de codificação, que seestende a partir dos nucleotídeos 2264 a 3450 e 5087 a 5354 de SEQ ID N0 45.

SEQ ID N0 49 é a seqüência de aminoácidos codificada por SEQID N0 48.

SEQ ID N0 50 é motivo de seqüência conservada útil naidentificação de genes pertencentes ao transportador de nitrato com altaafinidade de genes.

SEQ ID N0 51 é motivo de seqüência conservada útil naidentificação de genes pertencentes ao transportador de nitrato com altaafinidade de genes.

SEQ ID N0 52 é motivo de seqüência conservada útil naidentificação de genes pertencentes ao transportador de nitrato com altaafinidade de genes.

SEQ ID N0 53 é 1561 bp da seqüência que contém ORF(nucleotídeos 757 a 1368 (Parada)) que codifica polipeptídeo tipo NAR2 demilho (NAR2.1).

SEQ ID N0 54 é 612 bp da seqüência de codificação, que seestende a partir dos nucleotídeos 758 a 1369 (Parada) de SEQ ID N0 53.

SEQ ID N0 55 é a seqüência de aminoácidos codificada pelosnucleotídeos 758 a 1366 de SEQ ID N0 54.

SEQ ID N0 56 é 756 bp, que se estende a partir dos nucleotídeos1 a 756 do suposto promotor da seqüência exibida em SEQ ID N0 53.

SEQ ID N0 57 é 594 bp de ORF (nucleotídeos 1 a 594 (Parada))que codifica polipeptídeo tipo NAR2 (NAR2.2).

SEQ ID N0 58 é a seqüência de aminoácidos codificada pelosnucleotídeos 1 a 591 de ORF de SEQ ID N0 57.

SEQ ID N0 59 é o primer externo específico NAR2.1 utilizado noExemplo 6.

SEQ ID N0 60 é o primer interno específico NAR2.1 utilizado noExemplo 6.

SEQ ID N0 61 a 64 são os primers de seqüenciamento utilizadospara seqüenciar a região acima no fluxo promotora de NAR2.1.

SEQ ID N0 65 exibe 2917 bp adicionais do suposto promotor deNAR2.1.

SEQ ID N0 66 exibe 4498 bp do gene NAR2.1 completo, incluindointron que se estende a partir dos nucleotídeos 3655 a 3841.SEQ ID N0 67 é 3506 bp, que se estende a partir dos nucleotídeos1 a 3506 do suposto promotor da seqüência genômica de NAR2.1 exibida emSEQ ID N0 66.

SEQ ID N0 68 é 1014 bp, que se estende a partir dos nucleotídeos1001 a 2014 do suposto promotor da seqüência genômica de NAR2.1 exibidaem SEQ ID N0 66.

SEQ ID N0 69 é 1492 bp, que se estende a partir dos nucleotídeos2015 a 3506 do suposto promotor da seqüência genômica de NAR2.1 exibidaem SEQ ID N0 66.

SEQ ID N0 70 é 3621 bp do fragmento genômico isolado noExemplo 14.

SEQ ID N0 71 é 3236 bp do suposto promotor de Nar de B73, quese estende a partir dos nucleotídeos 1 a 3236 de SEQ ID N0 70.

SEQ ID N0 72 é 1000 bp do suposto promotor de Nar de B73, quese estende a partir dos nucleotídeos 1 a 1000 de SEQ ID N0 70.

SEQ ID N0 73 é 2236 bp do suposto promotor de Nar de B73, quese estende a partir dos nucleotídeos 1001 a 3236 de SEQ ID N0 70.

SEQ ID N0 74 é 1237 bp do suposto promotor de Nar de B73, quese estende a partir dos nucleotídeos 2000 a 3236 de SEQ ID N0 70.

SEQ ID N0 75 a 78 são os primers frontal e reverso descritos noExemplo 14.

SEQ ID N0 79 a 84 são os primers de seqüenciamento utilizadospara seqüenciar o promotor de Nar de B73 conforme descrito no Exemplo 14.

SEQ ID N0 85 é a seqüência do vetor pENTR-5' descrita noExemplo 14.

SEQ ID N0 86 é a seqüência do vetor PHP27621 descrita noExemplo 16.

SEQ ID N0 87 é a seqüência do vetor PHP27660 descrita noExemplo 17.

SEQ ID N0 88 é a seqüência do vetor PHP27860 descrita noExemplo 17.

SEQ ID N0 89 é 3324 bp do suposto promotor de Nar de B73, quecompreende os nucleotídeos 1 a 1523 e 1821 a 3324 de SEQ ID N0 70.

SEQ ID N0 90 é 500 bp do suposto promotor de Nar de B73, quese estende a partir dos nucleotídeos 2825 a 3324 de SEQ ID N0 70.

SEQ ID N0 91 representa 2025 bp da seqüência de milho quecontém ORF (nucleotídeos 250 a 1812 (Parada)) do gene que codifica otransportador de nitrato com alta afinidade (HAT5) isolado do clonecfp4n.pk008.p6:fis.

SEQ ID N0 92 é a seqüência de aminoácidos codificada por ORFde SEQ ID N0 91.

SEQ ID N0 93 é a seqüência do vetor PHP27280 descrita noExemplo 20.

SEQ ID N0 94 é a seqüência do vetor PHP27281 descrita noExemplo 20.

SEQ ID N0 95 é a seqüência do vetor PHP27282 descrita noExemplo 20.

SEQ ID N0 96 é a seqüência do vetor PHP27283 descrita noExemplo 20.

A Listagem de Seqüências contém o código de uma letra paracaracteres de seqüência de nucleotídeos e os códigos de três letras paraaminoácidos conforme definido de acordo com os padrões IUPAC-IUBMB descritosem NucleicAcids Research 13: 3021-3030 (1985) e em Biochemical Joumal 219 (n°2): 345-373 (1984), que são incorporados ao presente como referência. Ossímbolos e formato utilizados para dados de seqüência de nucleotídeos e deaminoácidos atendem às regras estabelecidas em 37 C. F. R. § 1.822.Descrição Detalhada da Invenção

O relatório descritivo de cada referência indicada no presente éintegralmente incorporado ao presente como referência.

O termo "NAR" indica genes relativos à assimilação de nitrato.Este tipo de genes e os polipeptídeos de NAR por eles codificados sãocomponente do sistema de absorção de nitrato com alta afinidade em plantas.

O termo "HAT" é utilizado de forma intercambiável comtransportador de nitrato com alta afinidade.

Da forma utilizada no presente, "fragmento de ácido nucléicoisolado" é utilizado de forma intercambiável com "polinucleotídeo isolado" e éum polímero de RNA ou DNA que possui fita simples ou dupla e contémopcionalmente bases de nucleotídeos sintéticas, não naturais ou alteradas.Fragmento de ácido nucléico isolado na forma de polímero de DNA pode sercomposto de um ou mais segmentos de cDNA, DNA genômico ou DNAsintético. Nucleotídeos (normalmente encontrados na sua forma de 5'-monofosfato) são indicados pela sua designação de letra única conformesegue: "A" para adenilato ou desoxiadenilato (para RNA ou DNA,respectivamente), "C" para citidilato ou desoxicitidilato, "G" para guanilato oudesoxiguanilato, "U" para uridilato, "T" para desoxitimidilato, "R" para purinas (Aou G), Ύ" para pirimidinas (C ou Τ), "K" para G ou Τ, Ή" para A ou C ou Τ, "I"para inosina e "N" para qualquer nucleotídeo.

O termo "isolado" designa materiais, tais como moléculas de ácidonucléico e/ou proteínas, que são substancialmente livres ou removidos de outraforma de componentes que normalmente acompanham ou interagem com osmateriais em ambiente de ocorrência natural. Os polinucleotídeos isoladospodem ser purificados a partir de célula hospedeira na qual ocorremnaturalmente. Métodos convencionais de purificação de ácido nucléicoconhecidos dos técnicos no assunto podem ser utilizados para a obtenção depolinucleotídeos isolados. A expressão também engloba polinucleotídeosrecombinantes e polinucleotídeos sintetizados quimicamente.

As expressões "subfragmento que é funcionalmente equivalente"e "subfragmento funcionalmente equivalente" são utilizadas de formaintercambiável no presente. Estas expressões designam uma parte ousubseqüência de fragmento de ácido nucléico isolado em que a capacidade dealteração da expressão genética ou produção de um certo fenótipo é retidacaso a parte ou subseqüência codifique ou não uma enzima ativa ou proteínafuncional (a parte ou subseqüência pode ser, por exemplo, uma parte deregiões de codificação e/ou não codificação e não necessita codificar umaenzima ativa ou proteína funcional). O fragmento ou subfragmento pode serutilizado, por exemplo, no projeto de construções de DNA recombinantes paraproduzir o fenótipo desejado em planta transformada. Construções de DNArecombinantes podem ser projetadas para uso em co-supressão ou semsentido por meio da ligação de um de seus fragmentos ou subfragmentos deácido nucléico, codifique ou não uma enzima ativa ou proteína funcional, naorientação apropriada com relação a uma seqüência promotora vegetal.

As expressões "homologia", "homólogo", "substancialmentesimilar" e "substancialmente correspondente" são utilizadas no presente deforma intercambiável. Elas designam fragmentos de ácidos nucléicos em quemodificações em uma ou mais bases de nucleotídeos não afetam a capacidadedo fragmento de ácido nucléico de mediar a expressão genética ou produzir umcerto fenótipo. Estas expressões também designam modificações dosfragmentos de ácidos nucléicos de acordo com a presente invenção, tais comoexclusão ou inserção de um ou mais nucleotídeos que não alteremsubstancialmente as propriedades funcionais do fragmento de ácido nucléicoresultante com relação ao fragmento não modificado inicial. Compreende-se,portanto, como apreciarão os técnicos no assunto, que a presente invençãoengloba mais que as seqüências de exemplos específicas.

Além disso, os técnicos no assunto reconhecem que seqüênciasde ácido nucléico substancialmente similares englobadas pela presenteinvenção também são definidas pela sua capacidade de hibridização sobcondições moderadamente estringentes (tais como 1 X SSC1 0,1% SDS, 60 0C)com as seqüências exemplificadas no presente, ou a qualquer parte dasseqüências de nucleotídeos relatadas no presente e que são funcionalmenteequivalentes ao gene ou ao promotor de acordo com a presente invenção. Ascondições de estringência podem ser ajustadas para selecionar fragmentosmoderadamente similares, tais como seqüências homólogas de organismoscom relacionamento distante, até fragmentos altamente similares, tais comogenes que duplicam enzimas funcionais de organismos intimamenterelacionados. Lavagens após a hibridização determinam as condições deestringência. Um conjunto de condições preferidas envolve uma série delavagens a partir de 6X SSC, 0,5% SDS à temperatura ambiente por quinzeminutos, repetidas em seguida com 2X SSC, 0,5% SDS a 45°C por trintaminutos, repetidas em seguida por duas vezes com 0,2X SSC, 0,5% SDS a50°C por trinta minutos. Um conjunto de condições estringentes de maiorpreferência envolve o uso de temperaturas mais altas em que as lavagens sãoidênticas às acima, exceto pela temperatura das duas lavagens finais de trintaminutos em 0,2X SSC, 0,5% SDS, que foi aumentada para 60°C. Outroconjunto preferido de condições altamente estringentes envolve o uso de duaslavagens finais em 0,1X SSC, 0,1% SDS a 65°C.

Com relação ao grau de similaridade substancial entre o mRNAalvo (endógeno) e a região de RNA na construção que possui homologia para omRNA alvo, essas seqüências deverão possuir pelo menos 25 nucleotídeos decomprimento, preferencialmente pelo menos cinqüenta nucleotídeos decomprimento, de maior preferência pelo menos cem nucleotídeos decomprimento, novamente de maior preferência pelo menos duzentosnucleotídeos de comprimento e, de preferência superior, pelo menos trezentosnucleotídeos de comprimento; e deverão ser pelo menos 80% idênticas,preferencialmente pelo menos 85% idênticas, de maior preferência pelo menos90% idênticas e, de preferência superior, pelo menos 95% idênticas.

Fragmentos de ácidos nucléicos substancialmente similarespodem ser selecionados por meio da seleção de fragmentos de ácidosnucléicos que representam subfragmentos ou modificações dos fragmentos deácidos nucléicos de acordo com a presente invenção, em que um ou maisnucleotídeos são substituídos, excluídos e/ou inseridos, pela sua capacidadede afetar o nível do polipeptídeo codificado pelo fragmento de ácido nucléiconão modificado em planta ou célula vegetal. Fragmento de ácido nucléicosubstancialmente similar que representa pelo menos trinta nucleotídeoscontíguos, preferencialmente pelo menos quarenta nucleotídeos contíguos, demaior preferência pelo menos sessenta nucleotídeos contíguos derivados dofragmento de ácido nucléico do presente, por exemplo, pode ser construído eintroduzido em planta ou célula vegetal. O nível do polipeptídeo codificado pelofragmento de ácido nucléico não modificado presente em planta ou célulavegetal exposta ao fragmento nucléico substancialmente similar pode sercomparado em seguida com o nível do polipeptídeo em planta ou célula vegetalque não é exposta ao fragmento de ácido nucléico substancialmente similar.

Alinhamentos seqüenciais e cálculos de similaridade percentualpodem ser determinados utilizando uma série de métodos de comparaçãoprojetados para detectar seqüências homólogas que incluem, mas sem Iimitar-se ao programa Megalign da suíte de computação bioinformática LASARGENE(DNASTAR Inc., Madison Wl, Estados Unidos). O alinhamento múltiplo dasseqüências é realizado utilizando o método de alinhamento Clustal V (Higgins eSharp (1989), CABIOS. 5: 151-153) com os parâmetros padrão (PENALIDADEDO INTERVALO = 10, PENALIDADE DO COMPRIMENTO DO INTERVALO =10). Os parâmetros padrão para alinhamentos em pares e cálculo dopercentual de identidade de seqüências de proteínas utilizando o métodoClustal são COMPRIMENTO DE PALAVRA = 1, PENALIDADE DOINTERVALO = 3, VISUALIZAÇÃO DO MELHOR RESULTADO = 5 eESPAÇAMENTO EM DIAGONAIS = 5. Para ácidos nucléicos, essesparâmetros são COMPRIMENTO DE PALAVRA = 1, PENALIDADE DOINTERVALO = 5, VISUALIZAÇAO DO MELHOR RESULTADO = 4 eESPAÇAMENTO EM DIAGONAIS = 4.

"Gene" indica fragmento de ácido nucléico que expressa proteínaespecífica, que inclui seqüências reguladoras anteriores (seqüências nãocodificadoras 5') e posteriores (seqüências não codificadoras 3') à seqüênciacodificadora. "Gene nativo" indica gene encontrado na natureza com suaspróprias seqüências reguladoras. "Construção de DNA recombinante" indicacombinação de fragmentos de ácido nucléico que normalmente não sãoencontrados juntos na natureza. Conseqüentemente, construção de DNArecombinante pode compreender seqüências reguladoras e seqüências decodificação que são derivadas de diferentes fontes, ou seqüências reguladorase seqüências de codificação derivadas da mesma fonte, mas dispostas demaneira diferente da normalmente encontrada na natureza. Gene "exógeno"indica gene normalmente não encontrado no organismo hospedeiro, mas que éintroduzido no organismo hospedeiro por meio de transferência genética. Osgenes exógenos podem compreender genes nativos inseridos em organismonão nativo ou construções de DNA recombinante. "Transgene" é gene que foiintroduzido no genoma por meio de procedimento de transformação.

"Seqüência codificadora" indica seqüência de DNA que codificaseqüência de aminoácidos específica. "Seqüências reguladoras" indicamseqüências de nucleotídeos localizadas acima (seqüências não codificadoras5'), na própria ou abaixo (seqüências não codificadores 3') de seqüênciacodificadora e que influenciam a transcrição, estabilidade ou processamento deRNA ou tradução da seqüência codificadora associada. As seqüênciasreguladoras podem incluir, mas sem limitar-se a promotores, seqüênciaslíderes de tradução, introns e seqüências de reconhecimento de poliadenilação.

"Promotor" designa seqüência de DNA capaz de controlar aexpressão de seqüência codificadora ou RNA funcional. A seqüênciapromotora consiste de elementos próximos e mais distantes no fluxo, com osúltimos elementos freqüentemente denominados amplificadores.Conseqüentemente, "amplificador" é seqüência de DNA que pode estimular aatividade promotora e pode ser elemento inato do promotor ou elementoheterólogo inserido para amplificar o nível ou a especificidade de tecido depromotor. As seqüências promotoras podem também estar localizadas dentrodas partes transcritas de genes e/ou abaixo no fluxo das seqüênciastranscritas. Os promotores podem ser derivados em sua totalidade de genenativo ou ser compostos de diferentes elementos derivados de diferentespromotores encontrados na natureza, ou mesmo compreendem segmentos deDNA sintéticos. Os técnicos no assunto compreendem que diferentespromotores podem dirigir a expressão de fragmento de ácido nucléico isoladoem diferentes tecidos ou tipos de células, em diferentes estágios dedesenvolvimento, ou em resposta a diferentes condições ambientais. Ospromotores que fazem com que fragmento de ácido nucléico isolado sejaexpresso na maior parte dos tipos de células na maior parte das vezes sãocomumente denominados "promotores constitutivos". Novos promotores dediversos tipos úteis em células vegetais estão constantemente sendorevelados; numerosos exemplos podem ser encontrados na compilação deOkamuro e Goldberg (1989), BiochemistryofPIants 15: 1-82.

Reconhece-se adicionalmente que, como na maior parte doscasos, as fronteiras exatas de seqüências reguladoras não foramcompletamente definidas, fragmentos de DNA com alguma variação podemapresentar atividade promotora idêntica. Da forma utilizada no presente,"subfragmento substancialmente similar e funcionalmente equivalente depromotor" indica uma parte ou subseqüência de seqüência promotora que écapaz de controlar a expressão de seqüência de codificação ou RNA funcional.

Exemplos específicos de promotores que podem ser úteis naexpressão dos fragmentos de ácidos nucléicos de acordo com a presenteinvenção incluem, mas sem limitar-se aos promotores descritos no presentepedido (SEQ ID N0 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73, 74, 89 ou 90).

"Intron" é seqüência interveniente em gene que não codifica parteda seqüência de proteína. Desta forma, estas seqüências são transcritas emRNA mas são extirpadas em seguida e não são traduzidas. O termo também éutilizado para as seqüências de RNA extirpadas.

Έχοη" é parte da seqüência de gene que é transcrita eencontrada no RNA mensageiro maduro derivado do gene, mas não énecessariamente parte da seqüência que codifica o produto genético final.

A expressão "seqüência de nucleotídeos deduzida" designaseqüência de DNA após a remoção das seqüências intervenientes, com basena homologia para outras seqüências de DNA que codificam a mesmaproteína.

A expressão "seqüência de aminoácidos deduzida" refere-se aseqüência de polipeptídeos derivada de seqüência de DNA após a remoção deseqüências intervenientes, com base na homologia para outras proteínascodificadas por seqüências de DNA que codificam a mesma proteína.

A expressão "seqüência líder de tradução" designa seqüência deDNA localizada entre a seqüência promotora de gene e a seqüênciacodificadora. A seqüência líder de tradução está presente no mRNA totalmenteprocessado após a seqüência inicial de tradução. A seqüência líder detradução pode afetar o processamento do transcrito primário em mRNA, aestabilidade do mRNA ou a eficiência da tradução. Foram descritos exemplosde seqüências líderes de tradução (Turner, R. e Foster1 G. D. (1995), MolecularBiotechnology 3: 225).

A expressão "seqüências não-codificadoras 3"' designaseqüências de DNA em local posterior a seqüência codificadora e incluiseqüências de reconhecimento de poliadenilação e outras seqüênciascodificadoras de sinais reguladores capazes de afetar o processamento demRNA ou a expressão genética. O sinal de poliadenilação é normalmentecaracterizado por afetar a adição de traços de ácido poliadenílico à extremidade 3'do precursor de mRNA. A utilização de diferentes seqüências não-codificadoras 3' éexemplificada por Ingelbrecht et al (1989), Plant Cell 1:671-680.

"Transcrito de RNA" designa o produto resultante da transcriçãocatalisada por polimerase de RNA de seqüência de DNA. Quando o transcritode RNA for cópia complementar perfeita da seqüência de DNA, ele édenominado transcrito primário ou pode ser seqüência de RNA derivada doprocessamento pós-transcricional do transcrito primário, sendo denominadoRNA maduro. "RNA mensageiro" ("mRNA") designa o RNA que não contémintrons e que pode ser traduzido em proteína pela célula. "cDNA" designa DNAque é complementar a modelo de mRNA e sintetizado a partir dele utilizando aenzima transcriptase reversa. O cDNA pode ser de fita única ou convertido emforma de fita dupla utilizando o fragmento Klenow de polimerase I de DNA.RNA "com sentido" designa transcrito de RNA que inclui o mRNA e pode sertraduzido em proteína em célula ou in vitro. "RNA sem sentido" designatranscrito de RNA que é complementar a mRNA ou transcrito primário alvo, notodo ou em parte, e que bloqueia a expressão de fragmento de ácido nucléicoisolado alvo (Patente Norte-Americana n° 5.107.065). A complementaridade deRNA sem sentido pode dar-se com qualquer parte do transcrito genéticoespecífico, ou seja, na seqüência não codificadora 5', seqüência nãocodificadora 3', introns ou na seqüência codificadora. "RNA funcional" designaRNA sem sentido, RNA de ribozima ou outro RNA que pode não ser traduzido,mas que ainda tenha efeito sobre processos celulares. As expressões"complemento" e "complemento reverso" são utilizadas de forma intercambiávelno presente com relação a transcritos de mRNA e destinam-se a definir o RNAsem sentido da mensagem.

A expressão "RNA endógeno" designa qualquer RNA que sejacodificado por qualquer seqüência de ácido nucléico presente no genoma dohospedeiro, seja de ocorrência natural ou não de ocorrência natural, ou seja,introduzida por meios recombinantes, mutagênese etc.

A expressão "não de ocorrência natural" indica artificial, nãoconsistente com o que normalmente é encontrado na natureza.

A expressão "ligado operativamente" designa a associação deseqüências de ácido nucléico sobre fragmento de ácido nucléico isolado, de talforma que a função de um seja regulada pelo outro. Promotor é ligadooperativamente a seqüência codificadora, por exemplo, quando for capaz deregular a expressão daquela seqüência codificadora (ou seja, que a seqüênciacodificadora esteja sob o controle transcricional do promotor). As seqüênciascodificadoras podem ser ligadas operativamente a seqüências reguladoras emorientação com sentido ou sem sentido. Em outro exemplo, as regiões de RNAcomplementar de acordo com a presente invenção podem ser ligadasoperativamente, seja direta ou indiretamente, 5' ao mRNA alvo, ou 3' ao mRNAalvo, ou no mRNA alvo, ou primeira região complementar é 5' e seucomplemento é 3' para o mRNA alvo.

O termo "expressão", da forma utilizada no presente, designa ageração de produto terminal funcional. A expressão de fragmento de ácidonucléico isolado envolve a transcrição do fragmento de ácido nucléico isolado etradução do mRNA em precursor ou proteína madura. "Inibição sem sentido"designa a produção de transcritos de RNA sem sentido capazes de suprimir aexpressão da proteína alvo. "Co-supressão" designa a produção de transcritos de RNA com sentido capazes de suprimir a expressão de genes endógenos ouexógenos idênticos ou substancialmente similares (Patente Norte-Americana n°5.231.020).

Proteína "madura" designa polipeptídeo processado após atradução, ou seja, aquele do qual quaisquer pré ou pró-peptídeos presentes noproduto de tradução original tenham sido removidos. Proteína "precursora"designa o produto original de tradução de mRNA; ou seja, com pré e pró-peptídeos ainda presentes. Os pré e pró-peptídeos podem ser, mas não selimitam a sinais de localização intracelular.

"Transformação estável" designa a transferência de fragmento deácido nucléico para o genoma de organismo hospedeiro, incluindo os genomasnucleares e organelares, resultando em herança geneticamente estável. Poroutro lado, "transformação transitória" indica a transferência de fragmento deácido nucléico para o núcleo, ou organela que contém DNA, de organismohospedeiro, resultando em expressão genética sem integração ou herançaestável. Os organismos hospedeiros que contêm os fragmentos de ácidosnucléicos transformados são denominados organismos "transgênicos". Ométodo preferido de transformação celular de arroz, milho e outrosmonocotiledôneos é o uso de tecnologia de transformação acelerada porpartículas ou por "disparador de genes" (Klein et al (1987), Nature (London)327: 70-73 (1987); Patente Norte-Americana n° 4.945.050) ou método mediadopor agrobactérias, utilizando plasmídeo de Ti apropriado que contém otransgene (Ishida, Y. et al, 1996, Nature Biotech., 14: 745-750). Os termos"transformação" e "transformado", da forma utilizada no presente, designamtransformação estável e transformação transitória.

As técnicas padrão de DNA recombinante e clonagem molecularutilizadas no presente são bem conhecidas na técnica e são descritas de formamais completa em Sambrook, J., Fritsch1 E. F. e Maniatis, T., MolecularCloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: ColdSpring Harbor, 1989 (a seguir, "Sambrook").

O termo "recombinante" designa combinação artificial de doissegmentos de seqüência de outra forma separados, por meio, por exemplo, desíntese química ou da manipulação de segmentos isolados de ácidos nucléicospor meio de técnicas de engenharia genética.

"PCR" ou "Reação em Cadeia de Polimerase" é técnica de síntesede grandes quantidades de segmentos de DNA específicos e consiste de umasérie de ciclos repetitivos (Perkin Elmer Cetus Instruments, Norwalk CT).Tipicamente, o DNA de fita dupla é desnaturado por calor, os dois primerscomplementares para as fronteiras 3' do segmento desejado são combinadossob baixa temperatura e estendidos em seguida sob temperatura intermediária.Um conjunto destas três etapas consecutivas é denominado ciclo.

Reação em cadeia de polimerase ("PCR") é técnica poderosautilizada para amplificar milhões de dobras de DNA, por meio da reproduçãorepetida de modelo em curto período de tempo (Mullis et al, Cold Spring HarborSymp. Quant. Bioi 51: 263-273 (1986); Erlich et al, Pedido de Patente Europeun° 50.424; Pedido de Patente Europeu n° 84.796; Pedido de Patente Europeun° 258.017; Pedido de Patente Europeu n° 237.362; Mullis, Pedido de PatenteEuropeu n° 201.184; Mullis et al, Patente Norte-Americana n° 4.683.202; Erlich,Patente Norte-Americana n° 4.582.788; e Saiki et al, Patente Norte-Americanan° 4.683.194). O processo utiliza conjuntos de oligonucleotídeos sintetizados invitro específicos para que sirvam de primer para a síntese de DNA. O projetodos primers depende das seqüências de DNA cuja análise é desejada. Atécnica é conduzida ao longo de vários ciclos (normalmente 20 a 50) de fusãodo modelo sob temperatura alta, o que permite que os primers se combinem aseqüências complementares no modelo e, em seguida, reproduzindo o modelocom polimerase de DNA.

Os produtos de reações de PCR são analisados por meio deseparação em géis de agarose seguida por manchas com brometo de etídio evisualização com transiluminação UV. Alternativamente, dNTPs radioativospodem ser adicionados ao PCR, a fim de incorporar marca aos produtos. Nestecaso, os produtos de PCR são visualizados por meio de exposição do gel afilme de raio X. A vantagem adicional de radiomarcar produtos de PCR é queos níveis de produtos de amplificação individuais podem ser quantificados.

As expressões "construção recombinante", "construção deexpressão" e "construção de expressão recombinante" são utilizadas de formaintercambiável no presente. Estas expressões designam unidade funcional dematerial genético que pode ser inserida no genoma de célula utilizandometodologia padrão bem conhecida dos técnicos no assunto. Esta construçãopode ser por si própria ou pode ser utilizada em conjunto com vetor. Caso sejautilizado vetor, a seleção do vetor depende do método a ser utilizado paratransformar as plantas hospedeiras, como é bem conhecido dos técnicos noassunto. Pode ser utilizado, por exemplo, vetor de plasmídeo. Os técnicos noassunto conhecem bem os elementos genéticos que necessitam estarpresentes sobre o vetor a fim de transformar, selecionar e propagar comsucesso células hospedeiras que compreendem qualquer dos fragmentos deácidos nucléicos isolados de acordo com a presente invenção. Os técnicos noassunto também reconhecerão que eventos diferentes e independentes detransformação resultarão em níveis e padrões diferentes de expressão (Joneset al (1985), EMBO J. 4: 2411-2418; De Almeida et al (1989), Moi Gen.Genetics 218: 78-86) e, portanto, que diversos eventos devem serselecionados a fim de obter-se linhagens que exibam o nível e padrão desejadode expressão. Esta seleção pode ser atingida por meio de análise Southern deDNA1 análise Northern de expressão de mRNA, análise Western de expressãode proteínas ou análise fenotípica.

Construções de co-supressão em plantas foram anteriormenteprojetadas concentrando-se na sobreexpressão de seqüência de ácido nucléicoque possui homologia para mRNA endógeno, na orientação com sentido, o queresulta na redução de todo o RNA que possui homologia para a seqüênciasobreexpressa (vide Vaucheret et al (1998), Plant J. 16: 651-659; e Gura(2000), Nature 404: 804-808). A eficiência geral deste fenômeno é baixa e aextensão da redução de RNA é amplamente variável. Trabalhos recentesdescreveram o uso de estruturas de "grampo de cabelo" que incorporam, notodo ou em parte, seqüência de codificação de mRNA em orientaçãocomplementar que resulta em potencial estrutura de "circuito de haste" para oRNA expresso (Patente Internacional PCT n0 WO 99/53050, publicada em 21de outubro de 1999). Isso aumenta a freqüência de co-supressão nas plantastransgênicas recuperadas. Outra variação descreve o uso de seqüências viraisde plantas para dirigir a supressão ou "silenciamento" de seqüências decodificação de mRNA próximas (Patente PCT n0 WO 98/36083, publicada em20 de agosto de 1998). Ambos estes fenômenos de co-supressão não foramelucidados mecanicamente, embora recentes evidências genéticas tenhamcomeçado a desemaranhar esta complexa situação (Elmayan et al (1998),Plant Ce//10: 1747-1757).

Em um aspecto, a presente invenção inclui polinucleotídeo isoladoque compreende seqüência de nucleotídeos que codifica polipeptídeonecessário para o transporte de nitrato com alta afinidade, em que opolipeptídeo contém seqüência de aminoácidos com pelo menos 80%, 85%,90%, 95% ou 99% de identidade de seqüências, com base no método dealinhamento Clustal V, em comparação com um dentre SEQ ID N0 36 ou 49. Opolipeptídeo pode também compreender SEQ ID N0 36 ou 49 e a seqüência denucleotídeos pode compreender SEQ ID N0 35 ou 48.

Também é incluído na presente invenção complemento dequalquer das seqüências de nucleotídeos acima, em que o complemento e aseqüência de nucleotídeos consistem da mesma quantidade de nucleotídeos esão 100% complementares.

Em outro aspecto, a presente invenção inclui polinucleotídeosisolados conforme descrito no presente (ou complementos), em que aseqüência de nucleotídeos compreende pelo menos dois, três, quatro ou cincomotivos selecionados a partir do grupo que consiste de SEQ ID N0 50, 51 e 52,em que o mencionado motivo é subseqüência substancialmente conservada.

"Motivos" ou "subseqüências" designam regiões curtas deseqüências conservadas de ácidos nucléicos ou aminoácidos que compreendem parte de seqüência mais longa. Espera-se, por exemplo, queessas subseqüências conservadas (tais como SEQ ID N0 50, 51 e 52) seriamimportantes para funcionamento e possam ser utilizadas para identificar novoshomólogos de homólogos de transportador de nitrato com alta afinidade emplantas. Espera-se que alguns ou todos os elementos possam ser encontradosem homólogo de transportador de nitrato com alta afinidade. Também seespera que pelo menos um ou dois dos aminoácidos conservados em qualquerdado motivo possam diferir de homólogo de transportador de nitrato com altaafinidade verdadeiro.

Em outro aspecto, polinucleotídeo de acordo com a presenteinvenção ou seu subfragmento funcionalmente equivalente é útil em inibiçãosem sentido ou co-supressão da expressão de seqüências de ácido nucléicoque codificam proteínas necessárias para transporte de nitrato com altaafinidade, de maior preferência em inibição sem sentido ou co-supressão detransportador de nitrato com alta afinidade endógeno ou gene transportador denitrato com alta afinidade heterólogo.

Protocolos para inibição sem sentido ou co-supressão são bemconhecidos dos técnicos no assunto e são descritos acima.

Em ainda outro aspecto, a presente invenção inclui fragmento deácido nucléico isolado que compreende (a) promotor que consisteessencialmente de SEQ ID N0 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73,74, 89 ou 90 ou (b) subfragmento substancialmente similar e funcionalmenteequivalente do mencionado promotor.

Também são de interesse construções de DNA recombinantesque compreendem qualquer dos fragmentos de ácidos nucléicos isoladosidentificados acima ou polinucleotídeos isolados, seus complementos ou partesdesses fragmentos ou complementos, ligados operativamente a pelo menosuma seqüência reguladora.

Plantas, tecido de plantas ou células de plantas quecompreendem essas construções de DNA recombinantes no seu genomatambém se encontram dentro do escopo da presente invenção. Métodos detransformação são bem conhecidos dos técnicos no assunto e são descritosacima. Qualquer planta, dicotiledônea ou monocotiledônea, pode sertransformada com essas construções de DNA recombinante.

Exemplos de monocotiledôneas incluem, mas sem limitar-se amilho, trigo, arroz, sorgo, milho branco, cevada, palma, lírio, Alstroemeria,centeio e aveia. Exemplos de dicotiledôneas incluem, mas sem limitar-se asoja, colza, girassol, canola, uva, guaiule, aquilégia, algodão, fumo, ervilha,feijão, linho, açafrão e alfafa.

Tecido vegetal inclui tecidos ou plantas diferenciados e nãodiferenciados, que incluem, mas sem limitar-se a raízes, hastes, brotos, folhas,pólen, sementes, tecidos tumorosos e várias formas de células e cultivo taiscomo células isoladas, protoplasma, embriões e tecido de calo. O tecidovegetal pode estar em planta ou em cultivo de órgãos, tecidos ou células.

Em outro aspecto, a presente invenção inclui método de alteraçãode transporte de nitrato das plantas, que compreende:

a. transformação de planta com construção de DNArecombinante que compreende:

i. construção de DNA recombinante que compreendepolinucleotídeo isolado que codifica polipeptídeo de HAT1 ligadooperativamente a pelo menos uma seqüência reguladora; e

ii. pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado que codifica polipeptídeo deNAR, ligado operativamente a pelo menos uma seqüência reguladora; e

b. cultivo da planta transformada de a sob condiçõesapropriadas para a expressão da construção de DNA recombinante; e seleçãodas plantas transformadas que possuem transporte de nitrato alterado.

Conforme utilizado no presente, a alteração do transporte denitrato das plantas pode resultar em aumento ou redução das alterações.

A regeneração, desenvolvimento e cultivo de plantas a partir detransformadores de protoplastas de plantas isoladas ou de vários explantestransformados é bem conhecida na técnica (Weissbach e Weissbach, Methodsfor Plant Molecular Biology (Eds.), Academic Press, Inc., San Diego CA(1988)). Este processo de regeneração e crescimento inclui tipicamente asetapas de seleção de células transformadas, cultivo das célulasindividualizadas ao longo dos estágios habituais de desenvolvimentoembriônico ao longo do estágio de plantícula enraizada. Os embriões esementes transgênicas são regenerados de forma similar. Os brotosenraizados transgênicos resultantes são plantados em seguida em meio decultivo vegetal apropriado tal como solo.O desenvolvimento ou regeneração de plantas que contêm ofragmento de ácido nucléico isolado exógeno externo que codifica proteína deinteresse é bem conhecido na técnica. Preferencialmente, as plantasregeneradas são autopolinizadas para fornecer plantas transgênicashomozigóticas. Caso contrário, o pólen obtido das plantas regeneradas écruzado para plantas cultivadas com sementes de linhagens agronomicamenteimportantes. Por outro lado, pólen de plantas dessas linhagens importantes éutilizado para polinizar plantas regeneradas. Planta transgênica de acordo coma presente invenção que contém polipeptídeo desejado é cultivada utilizandométodos bem conhecidos dos técnicos no assunto.

Existe uma série de métodos de regeneração de plantas a partirde tecido vegetal.

O método específico de regeneração dependerá do tecido vegetalinicial e da espécie vegetal específica a ser regenerada.

Métodos de transformação de dicotiledôneas, principalmente pormeio do uso de Agrobacterium tumefaciens, e obtenção de plantastransgênicas foram publicados para algodão (Patente Norte-Americana n°5.004.863, Patente Norte-Americana n° 5.159.135, Patente Norte-Americana n°5.518.908); soja (Patente Norte-Americana n° 5.569.834, Patente Norte-Americana n° 5.416.011, McCabe et al, Bio/Technology 6: 923 (1988), Christouet al, Plant Physiol. 87: 671-674 (1988)); Brassica (Patente Norte-Americana n°5.463.174), amendoim (Cheng et al, Plant Cell Rep. 15: 653-657 (1996);McKentIy et al, Plant Cell Rep. 14: 699-703 (1995)); mamão e ervilha (Grant etal, Plant Cell Rep. 15: 254-258 (1995)).

A transformação de monocotiledôneas utilizando eletroporação,bombardeamento de partículas e Agrobacterium também foi relatada.Transformação e regeneração vegetal foram atingidas em aspargos (Bytebieret al, Proc. Natl. Acad. Sei. (U. S. A.) 84: 5354 (1987)); cevada (Wan e Lemaux,Plant Physioi 104: 37 (1994)); Zea mays (Rhodes et al, Science 240: 204(1988), Gordon-Kamm et al, Plant Cell 2: 603-618 (1990), Fromm et al,Bio/Technology 8: 833 (1990), Koziel et al, Bio/Technology 11: 194 (1993),Armstrong et al, Crop Science 35: 550-557 (1995)); aveia (Somers et al,Bio/Technology 10: 15-89 (1992)); grama de orquídea (Horn et al, Plant CellRep. 7: 469 (1988)), arroz (Toriyama et al, Theor. Appi Genet. 205: 34 (1986);Part et al, Plant Mol. Bioi 32: 1135-1148 (1996); Abedinia et al, Aust. J. PlantPhysiol. 24: 133-141 (1997); Zhang e Wu, Theor. Appl. Genet. 76: 835 (1988);Zhang et al, Plant Cell Rep. 7: 379 (1988); Battraw e Halll Plant Sei. 86: 191-202 (1992); Christou et al, Bio/Technology 9: 957 (1991)); centeio (De La Penaet al, Nature 325: 274 (1987)); cana de açúcar (Bower e Birch, Plant J. 2: 409(1992); festuca alta (Wang et al, Bio/Technology 10: 691 (1992)) e trigo (Vasilet al, Bio/Technology 10: 667 (1992); Patente Norte-Americana n° 5.631.152).

Testes de expressão genética com base na expressão transitóriade construções de ácido nucléico clonado foram desenvolvidos por meio daintrodução das moléculas de ácido nucléico em células vegetais por meio detratamento com polietileno glicol, eletroporação ou bombardeamento departículas (Marcotte et al, Nature 335: 454-457 (1988), Marcotte et al, Plant Cell1: 523-532 (1989); McCarty et al, Cell 66: 895-905 (1991), Hattori et al, GenesDev. 6: 609-618 (1992); Goff et al, EMBO J. 9: 2517-2522 (1990)).

Sistemas de expressão transitória podem ser utilizados paradissecar funcionalmente construções de fragmentos de ácidos nucléicosisolados (vide, de forma geral, Maliga et al, Methods in Plant Molecular Biology,Cold Spring Harbor Press (1995)). Compreende-se que qualquer das moléculasde ácido nucléico de acordo com a presente invenção pode ser introduzida emcélulas vegetais de maneira transitória ou permanente em combinação comoutros elementos genéticos tais como vetores, promotores, amplificadores etc.

Além dos procedimentos discutidos acima, os praticantes sãofamiliares com os materiais de recursos padrão que descrevem condiçõesespecíficas e procedimentos de construção, manipulação e isolamento demacromoléculas (tais como moléculas de DNA1 plasmídeos etc.), geração deorganismos recombinantes e seleção e isolamento de clones (vide, porexemplo, Sambrook et al, Molecular Cloning: A Laboratory Manual, Cold SpringHarbor Press (1989); Maliga et al, Methods in Plant Molecular Biology, ColdSpring Harbor Press (1995); Birren et al, GenomeAnaIysis: Detecting Genes, 1,Cold Spring Harbor, Nova Iorque (1998); Birren et al, Genome Analysis:Analyzing DNA, 2, Cold Spring Harbor, Nova Iorque (1998); Plant MolecularBiology: A Laboratory Manual, eds. Clark, Springer, Nova Iorque (1997)).

Em ainda outro aspecto, a presente invenção inclui método deisolamento de fragmentos de ácidos nucléicos que codificam polipeptídeosassociados à alteração de transporte de nitrato das plantas, que compreende:

(a) comparação de SEQ ID N0 36 ou 49 com outras seqüências de polipeptídeos associadas à alteração de transporte de nitrato de

plantas;

(b) identificação de seqüências conservadas de quatro ou mais

aminoácidos obtidos na etapa (a);

(c) elaboração de oligômero(s) ou sonda(s) de nucleotídeosespecíficos de regiões com base nas seqüências conservadas identificadas na

etapa (b); e

(d) uso do(s) oligômero(s) ou sonda(s) de nucleotídeos daetapa (c) para isolar seqüências associadas à alteração do transporte de nitratodas plantas por meio de protocolos dependentes de seqüências.

Exemplos de elementos de seqüências conservados que seriamúteis na identificação de outras seqüências vegetais associadas à alteração dotransporte de nitrato de plantas podem ser encontrados no grupo quecompreende, mas sem limitar-se aos nucleotídeos que codificam ospolipeptídeos de SEQ ID N0 50, 51 e 52.

Em outro aspecto, a presente invenção também inclui método demapeamento de variações genéticas relativas à alteração de transporte denitrato de plantas, que compreende:

(a) cruzamento de duas variedades de plantas; e

(b) avaliação de variações genéticas com relação a:

(i) seqüência de ácidos nucléicos selecionada a partirdo grupo que consiste de SEQ ID N0 35 e 48; ou

(ii) seqüência de ácidos nucléicos que codificapolipeptídeo selecionado a partir do grupo que consiste de SEQ ID N0 36 e 49em plantas progenitoras resultantes do cruzamento da etapa (a) em que aavaliação é realizada utilizando método selecionado a partir do grupo queconsiste de: análise RFLP, análise SNP e análise com base em PCR.

Em outra realização, a presente invenção inclui método desemeadura molecular para obter transporte de nitrato das plantas alterado:

(a) cruzamento de duas variedades de plantas; e

(b) avaliação de variações genéticas com relação a:

(i) seqüência de ácidos nucléicos selecionada a partirdo grupo que consiste de SEQ ID N0 35 e 48; ou

(ii) seqüência de ácidos nucléicos que codificapolipeptídeo selecionado a partir do grupo que consiste de SEQ ID N0 36 e 49;

em plantas progenitoras resultantes do cruzamento da etapa (a)em que a avaliação é realizada utilizando método selecionado a partir do grupoque consiste de: análise RFLP, análise SNP e análise com base em PCR.

As expressões "variação genética de mapeamento" ou "variabilidadegenética de mapeamento" são utilizadas de forma intercambiável e definem oprocesso de identificação de alterações de seqüências de DNA1 seja de causasnaturais ou induzidas, em região genética que diferencia entre diferenteslinhagens vegetais, cultivos, variedades, famílias ou espécies. A variabilidadegenética em local específico (gene) devido até mesmo a mudanças de basemenores pode alterar o padrão de fragmentos de digestão de enzimas de restriçãoque podem ser geradas. Alterações patogênicas do genótipo podem dever-se aexclusões ou inserções no gene sendo analisado ou mesmo substituições denucleotídeos isolados que podem criar ou excluir local de reconhecimento deenzimas de restrição. Análise RFLP (polimorfismos de comprimento de fragmentode restrição) utiliza isso e emprega Southem Blot com uma sonda correspondenteao fragmento de ácido nucléico isolado de interesse.

Desta forma, caso polimorfismo (ou seja, variação de ocorrêncianatural em gene ou segmento de DNA; além disso, a existência de váriasformas de gene (alelos) na mesma espécie) crie ou destrua local de divisão deendonuclease de restrição, ou caso resulte na perda ou inserção de DNA (talcomo polimorfismo de repetição conjunta de nucleotídeos variáveis (VNTR), elealterará o tamanho ou o perfil dos fragmentos de DNA que são gerados pormeio de digestão com aquela endonuclease de restrição. Desta forma,indivíduos que possuam seqüência variante podem ser diferenciados dos quepossuem a seqüência original por meio de análise de fragmentos de restrição.

Polimorfismos que podem ser identificados desta maneira são denominadosRFLPs. RFLPs vêm sendo amplamente utilizados em análises genéticasvegetais e humanas (Glassberg, Pedido de Patente Britânico n° 2.135.774;Skolnick et al, Cytogen, Cell Genet. 32: 58-67 (1982); Botstein et al, Ann. J.Hum. Genet. 32: 314-331 (1980); Fischer et al (Pedido PCT n0 WO 90/13668,Uhlen, Pedido PCT n0 WO 90/11369).

Atributo central de "polimorfismos de nucleotídeos isolados" ou"SNPs" é que o local do polimorfismo encontra-se em nucleotídeo isolado. OsSNPs possuem certas vantagens relatadas sobre RFLPs ou VNTRs. Emprimeiro lugar, os SNPs são mais estáveis que outras classes depolimorfismos. A sua velocidade de mutação espontânea é de cerca de 10"9(Kornberg1 DNA Replication, W. H. Freeman & Co., San Francisco, 1980),cerca de mil vezes menos freqüente que VNTRs (Patente Norte-Americana n°5.679.524). Em segundo lugar, SNPs ocorrem em freqüência maior e commaior uniformidade que RFLPs e VNTRs. Como SNPs resultam de variação deseqüências, seqüenciamento de moléculas de cDNA ou genômicas aleatóriaspode identificar novos polimorfismos. SNPs podem também resultar deexclusões, mutações pontuais e inserções. Qualquer alteração de base isolada,seja qual for a causa, pode ser SNP. A maior freqüência de SNPs significa que eles podem ser identificados mais facilmente que as outras classes depolimorfismos.

SNPs podem ser caracterizados utilizando qualquer dentre umasérie de métodos. Esses métodos incluem o seqüenciamento direto ou indiretodo local, o uso de enzimas de restrição em que os alelos correspondentes dolocal criam ou destroem local de restrição, o uso de sondas de hibridizaçãoespecíficas de alelos, o uso de anticorpos que são específicos para asproteínas codificadas pelos diferentes alelos do polimorfismos ou por outrainterpretação bioquímica. SNPs podem ser seqüenciados por meio de umasérie de métodos. Dois métodos básicos podem ser utilizados paraseqüenciamento de DNA, o método de término de cadeias de Sanger et al,Proc. Nati Acad. Sei. (U. S. A.) 74: 5463-5467 (1977) e o método dedegradação química de Maxam e Gilbert, Proc. Natl. Acad. Sei. (U. S. A.) 74:560-564 (1977).

Além disso, mutações de pontos isolados podem ser detectadaspor meio de métodos de PCR modificados tais como reação em cadeia deligase ("LCR") e análise de polimorfismos de conformação de fita única-PCR("PCR-SSCP"). O método de PCR pode também ser utilizado para identificar onível de expressão de genes em amostras extremamente pequenas dematerial, tais como tecidos ou células de corpo. O método é denominadotranscrição reversa-PCR ("RT-PCR").

A expressão "cultivo molecular" define o processo derastreamento de marcadores moleculares durante o processo de cultivo. Écomum que os marcadores moleculares sejam ligados a característicasfenotípicas que são desejáveis. Seguindo-se a segregação do marcadormolecular ou característica genética, em vez de avaliar fenótipo, o processo decultivo pode ser acelerado por meio de cultivo de menos plantas e eliminaçãodo teste ou inspeção visual em busca de variação fenotípica. Os marcadoresmoleculares úteis neste processo incluem, mas sem limitar-se a qualquermarcador útil na identificação de variações genéticas mapeáveis mencionadasanteriormente, bem como quaisquer genes ligados intimamente que exibemsintenia entre as espécies vegetais. O termo "sintenia" designa a conservaçãode ordem/colocação genética sobre cromossomos entre diferentes organismos.Isso significa que dois ou mais locais genéticos, que podem ou não ser ligadosintimamente, são encontrados sobre o mesmo cromossomo entre espéciesdiferentes. Outra expressão para sintenia é "colinearidade de genoma".

Os fragmentos de ácidos nucléicos de acordo com a presenteinvenção podem ser utilizados para criar plantas transgênicas nas quais ospolipeptídeos descritos estão presentes em níveis mais altos ou mais baixosque os normais ou em tipos de células ou etapas de desenvolvimento em quenormalmente não são encontradas. Isso teria o efeito de alterar o nível detransporte de nitrogênio e acúmulo nessas células. Deficiência de nitrogênioem plantas resulta em crescimento retardado e, muitas vezes, em hastesdelgadas e freqüentemente lenhosas. Em muitas plantas, o primeiro sinal dedeficiência de nitrogênio é clorose (amarelamento das folhas).

A sobreexpressão das proteínas de acordo com a presenteinvenção pode ser obtida elaborando-se em primeiro lugar construção de DNArecombinante na qual a região de codificação é ligada operativamente apromotor capaz de dirigir a expressão de gene nos tecidos desejados na etapade desenvolvimento desejada. Por motivos de conveniência, a construção deDNA recombinante pode compreender seqüências promotoras e seqüênciaslíderes de tradução derivadas dos mesmos genes. Seqüências nãocodificadoras 3' que codificam sinais de término de transcrição podem tambémser fornecidas. A construção de DNA recombinante do presente pode tambémcompreender um ou mais introns, a fim de facilitar a expressão genética.

Podem ser elaborados vetores de plasmídeos que compreendem aconstrução de DNA recombinante do presente. A seleção de vetor de plasmídeodepende do método que será utilizado para transformar plantas hospedeiras. Ostécnicos no assunto conhecem bem os elementos genéticos que devem estarpresentes sobre o vetor de plasmídeo para transformar, selecionar e propagarcom sucesso células hospedeiras que contêm a construção de DNArecombinante. Os técnicos no assunto também reconhecerão que diferenteseventos de transformação independentes resultarão em níveis e padrões deexpressão diferentes (Jones et al (1985), EMBO J. 4: 2411-2418; de Almeida et al(1989), Moi Gen. Genetics 218: 78-86) e, portanto, que diversos eventos devemser selecionados a fim de obter linhagens que exibam o padrão e nível deexpressão desejado. Esta seleção pode ser realizada por meio de análiseSouthern de DNA, análise Northern da expressão de mRNA, análise Western deexpressão de proteínas ou análise fenotípica.

Para algumas aplicações, pode ser útil dirigir os polipeptídeos dopresente para diferentes compartimentos celulares, ou facilitar a sua secreçãoda célula. Idealiza-se, portanto, que a construção de DNA recombinantedescrita acima pode ser adicionalmente suplementada por meio da alteraçãoda seqüência de codificação para codificar os polipeptídeos do presente comseqüências de direcionamento intracelular apropriadas, tais como seqüênciasde trânsito (Keegstra (1989), Cell 56: 247-253), seqüências de sinal ouseqüências que codificam a localizam do retículo endoplasmático (Chrispeels(1991), Ann. Rev. Plant Phys. Plant Mol. Bioi 42: 21-53) ou sinais delocalização nuclear (Raikhel (1992), Plant Phys. 100: 1627-1632) adicionadose/ou com seqüências de direcionamento que já são removidas no presente.Embora as referências mencionadas forneçam exemplos de cada um destes, alista não é exaustiva e mais sinais de direcionamento úteis podem serrevelados no futuro.

Pode também ser desejável reduzir ou eliminar a expressão degenes que codificam os polipeptídeos do presente em plantas para algumasaplicações. A fim de conseguir isso, construção de DNA recombinanteprojetada para co-supressão do polipeptídeo do presente pode ser elaboradapor meio de ligação de gene ou fragmento genético que codifica aquelepeptídeo para seqüências promotoras de plantas. Alternativamente, construçãode DNA recombinante projetada para expressar RNA sem sentido para ofragmento de ácido nucléico d o presente, no todo ou em parte, pode serelaborada por meio de ligação do gene ou fragmento de gene em orientaçãoreversa a seqüências promotoras de plantas. As construções de DNArecombinante de co-supressão ou sem sentido poderão ser introduzidas emplantas por meio de transformação, em que a expressão dos genes endógenoscorrespondentes é reduzida ou eliminada.

Soluções genéticas moleculares para a geração de plantas comexpressão genética alterada possuem vantagem decisiva sobre abordagens decultivo de plantas mais tradicionais. Alterações dos fenótipos das plantaspodem ser produzidas por meio da inibição específica da expressão de um oumais genes por meio de inibição sem sentido ou co-supressão (Patentes Norte-Americanas n° 5.190.931, 5.107.065 e 5.283.323). Construção sem sentido oude co-supressão agiria como regulador negativo dominante da atividadegenética. Embora mutações convencionais possam gerar regulagem negativada atividade genética, estes efeitos são mais provavelmente recessivos. Aregulagem genética dominante disponível com abordagem transgênica podeser vantajosa do ponto de vista de cultivo. Além disso, a capacidade derestrição da expressão de fenótipo específico aos tecidos reprodutores daplanta utilizando promotores específicos de tecidos pode conferir vantagensagronômicas com relação às mutações convencionais, o que pode ter efeito emtodos os tecidos nos quais gene mutante normalmente é expresso.

Os técnicos no assunto saberão que considerações especiais sãoassociadas ao uso de tecnologias de co-supressão ou sem sentido, a fim dereduzir a expressão de genes específicos. O nível adequado de expressão degenes com ou sem sentido, por exemplo, pode exigir o uso de diferentesconstruções de DNA recombinante utilizando elementos reguladores diferentesconhecidos dos técnicos no assunto. Após a obtenção de plantas transgênicaspor meio de um dos métodos descritos acima, será necessário selecionartransgênicos individuais em busca dos que exibam mais eficientemente ofenótipo desejado. Conseqüentemente, os técnicos no assunto desenvolverãométodos de seleção de grandes quantidades de transformadores. A naturezadestas seleções será geralmente selecionada em bases práticas e não é parteinerente da presente invenção. Pode-se selecionar, por exemplo, buscandoalterações da expressão genética utilizando anticorpos específicos para aproteína codificada pelo gene sendo suprimido, ou pode-se estabelecer testesque meçam especificamente a atividade enzimática. Método preferido seráaquele que permite que grandes quantidades de amostras sejam processadasrapidamente, pois esperar-se-á que grande quantidade de transformadoresseja negativa para o fenótipo desejado.

Os polipeptídeos do presente (ou suas partes) podem serproduzidos em células hospedeiras heterólogas, particularmente nas células dehospedeiros microbianos, e podem ser utilizados para preparar anticorpos paraessas proteínas por meio de métodos bem conhecidos dos técnicos noassunto. Os anticorpos são úteis para detectar os polipeptídeos de acordo coma presente invenção in situ em células ou in vitro em extratos de células.Células hospedeiras heterólogas preferidas para a produção dos polipeptídeosdo presente são hospedeiros microbianos. Sistemas de expressão microbianose vetores de expressão que contêm seqüências reguladoras que dirigemexpressão de alto nível de proteínas exógenas são bem conhecidos dostécnicos no assunto. Qualquer destes poderá ser utilizado para elaborar construção de DNA recombinante para a produção dos polipeptídeos dopresente. Esta construção de DNA recombinante poderá ser introduzida emseguida em microorganismos apropriados por meio de transformação parafornecer expressão de alto nível do transportador de amônio codificado. Éfornecido exemplo de vetor para expressão de alto nível dos polipeptídeos do presente em hospedeiro bacteriano (Exemplo 7).

Além disso, os polipeptídeos do presente podem ser utilizadoscomo alvos para possibilitar o projeto e/ou identificação de inibidores dessasenzimas que podem ser úteis como herbicidas. Isso é desejável porque ospolipeptídeos descritos no presente catalisam várias etapas da absorção de nitrogênio. Conseqüentemente, a inibição da atividade de uma ou mais dasenzimas descritas no presente poderá gerar a inibição do crescimento daplanta. Desta forma, os polipeptídeos do presente poderão ser apropriadospara a revelação e projeto de novos herbicidas.

Os fragmentos de ácidos nucléicos de acordo com a presenteinvenção, no todo ou em parte substancial, podem também ser utilizados comosondas para mapeamento físico e genético dos genes de que são parte e comomarcadores para características ligadas a esses genes. Estas informaçõespodem ser úteis no cultivo de plantas, a fim de desenvolver linhagens comfenótipos desejados. Os fragmentos de ácido nucléico do presente podem serutilizados, por exemplo, como marcadores de polimorfismo de comprimento defragmento de restrição (RFLP). Manchas Southern (Maniatis) de DNAgenômico de plantas digerido por restrição podem ser sondadas com osfragmentos de ácido nucléico de acordo com a presente invenção. Os padrõesde faixas resultantes podem ser submetidos em seguida a análises genéticasutilizando programas de computador tais como MapMaker (Lander et al (1987),Genomics 1: 174-181), a fim de construir mapa genético. Além disso, osfragmentos de ácidos nucléicos de acordo com a presente invenção podem serutilizados para sondar manchas Southern que contêm DNAs genômicostratados com endonuclease de restrição de conjunto de indivíduos querepresentam pais e prole de cruzamento genético definido. A segregação dospolimorfismos de DNA é observada e utilizada para calcular a posição daseqüência de ácido nucléico do presente no mapa genético obtidoanteriormente utilizando esta população (Botstein et al (1980), Am. J. Hum.Genet. 32: 314-331).

A produção e uso de sondas derivadas de genes de plantas parauso em mapeamento genético é descrita em Bernatzky e Taknsley (1986),Plant Mol. Bioi Repórter 4 (1): 37-41. Numerosas publicações descrevem omapeamento genético de clones de cDNA específicos utilizando a metodologiadescrita acima ou suas variações. Populações intercruzadas F2, por exemplo,populações de cruzamentos anteriores, populações acasaladas aleatoriamente,linhagens próximas da isogênica e outros conjuntos de indivíduos podem serutilizados para mapeamento. Estas metodologias são bem conhecidas dostécnicos no assunto.

Pode-se também utilizar sondas de ácidos nucléicos derivadasdas seqüências de ácidos nucléicos do presente para mapeamento físico (ouseja, colocação de seqüências sobre mapas físicos; vide Hoheisel et al,Nonmammalian Genomic Analysis: A Practical Guide\ Academic Press, 1996;págs. 319-346 e referências ali mencionadas).

Em outra realização, sondas de ácidos nucléicos derivadas dasseqüências de ácidos nucléicos do presente podem ser utilizadas emmapeamento por hibridização in situ por fluorescência direta (FISH) (Trask(1991), Trends Genet. 7: 149-154). Embora os métodos atuais de mapeamentopor FISH favoreçam a utilização de clones grandes (vários a várias centenasde kb; vide Laan et al (1995), Genome Res. 5: 13-20), aprimoramentos desensibilidade podem permitir a realização de mapeamento por FISH utilizandosondas mais curtas.

Uma série de métodos com base em amplificação de ácidosnucléicos de mapeamento físico e genético pode ser conduzida utilizando asseqüências de ácidos nucléicos do presente. Exemplos incluem amplificaçãoespecífica de alelos (Kazazian (1989), J. Lab. Clin. Med. 11: 95-96),polimorfismo de fragmentos amplificados por PCR (CAPS; Sheffield et al(1993), Genomics 16: 325-332), ligação específica de alelos (Landegren et al(1988), Science 241: 1077-1080), reações de extensão de nucleotídeos(Sokolov (1990), Nucleic Acid Res. 18: 3671), Mapeamento Híbrido deRadiação (Walter et al (1997), Nature Geneties 7: 22-28) e Mapeamento Happy(Dear e Cook (1989), Nueleie Aeid Res. 17: 6795-6807). Para estes métodos,utiliza-se a seqüência de fragmento de ácido nucléico para projetar e produzirpares de primers para uso na reação de amplificação ou em reações deextensão de primers. O projeto desses primers é bem conhecido dos técnicosno assunto. Em métodos que empregam mapeamento genético com base emPCR, pode ser necessário identificar diferenças de seqüências de DNA entreos pais da cruz de mapeamento na região correspondente à seqüência deácido nucléico do presente. Isso, entretanto, geralmente não é necessário paramétodos de mapeamento.A perda de fenótipos mutantes de função pode ser identificada paraos clones de cDNA do presente por meio de protocolos de rompimento de genesdirecionados ou da identificação de mutantes específicos para os genes contidosem população de milho que conduz mutações em todos os genes possíveis(Ballinger e Benzer (1989), Proc. Nati Acad. Sei. U. S. A. 86: 9402-9406; Koes etal (1995), Proc. Natl. Acad. Sei. U. S. A. 92: 8149-8153; Bensen e al (1995),Plant Cell 7: 75-84). Esta última abordagem pode ser atingida de duas formas.

Primeiramente, os segmentos curtos dos fragmentos de ácidos nucléicos dopresente podem ser utilizados em protocolos de reação em cadeia de polimeraseem conjunto com primer de seqüência de marca de mutação sobre DNAspreparados a partir de população de plantas em que transposons mutantes oualgum outro elemento de DNA causador de mutação tenha sido introduzido (videBensen, acima). A amplificação de um fragmento de DNA específico com essesprimers indica a inserção do elemento de marca de mutação no gene vegetalcodificador dos polipeptídeos do presente ou perto dele. Alternativamente, ofragmento de ácido nucléico do presente pode ser utilizado na forma de sondade hibridização contra produtos de amplificação de PCR gerados a partir dapopulação em mutação, utilizando o primer de seqüência de marca de mutaçãoem conjunto com primer de local genômico arbitrário, tal como para adaptadorsintético ancorado a local da enzima de restrição. Com qualquer dos métodos,pode ser identificada e obtida uma planta que contém mutação no geneendógeno codificador dos polipeptídeos do presente. Esta planta mutante podeser utilizada em seguida para determinar ou confirmar a função natural dospolipeptídeos do presente aqui descritos.

A função dos transportadores de nitrato com alta afinidade epolipeptídeos necessários para transporte de nitrato com alta afinidade podeser confirmada utilizando a população mutante TUSC. O Sistema de Utilidadede Características para Milho (TUSC) é método que emprega técnicasmoleculares e genéticas para facilitar o estudo de função genética em milho. Oestudo da função genética indica que a seqüência do gene já é conhecida e,desta forma, o método funciona na direção contrária: da seqüência para ofenótipo. Este tipo de aplicação é denominado "genética reversa", ao contráriodos métodos "para a frente" (tais como marcação de transposons) que sãoprojetados para identificar e isolar o(s) gene(s) responsável(is) porcaracterística específica (fenótipo).

A Pioneer Hi-Bred International, Inc. possui a sua coleçãoparticular de DNA genômico de milho de cerca de 42.000 plantas F1 individuais(Reverse Genetics for Maize\ Meeley, R. e Briggs, S., 1995, Maize Genet.

Coop. Newslett. 69: 67, 82).

O genoma de cada um destes indivíduos contém diversas cópiasda família de elementos transportáveis, Mutante (Mu). A família Mu é altamentemutagênica; na presença do elemento ativo Mu-DR, estes elementostransportam-se ao longo de todo o genoma, inserindo em regiões gênicas e,freqüentemente, rompendo a função genética. Ao recolher DNA genômico degrande quantidade de indivíduos (42.000), a Pioneer reuniu biblioteca dogenoma de milho que sofreu mutagênese. Eventos de inserção de Mu sãopredominantemente heterozigóticos, de forma que, dada a natureza recessivada maior parte das mutações de inserção, as plantas Fi parecerão do tiposelvagem. Cada uma das plantas foi isolada para produzir semente de F2, quefoi recolhida. Ao gerar a prole de F2, mutações de inserção segregam-se emforma de Mendel e, portanto, são úteis para a investigação do efeito de alelomutante sobre o fenótipo. O sistema TUSC vem sendo utilizado com sucessopor uma série de laboratórios para identificar a função de uma série de genes(Cloning and Characterization of the Maize An1 Gene, Bensen, R. J. et al,1995, Plant Cell 7: 75-84; Diversification of C-Function Aetivity in Maize FlowerDevelopment, Mena, M. et al, 1996, Science 274: 1537-1540; Analysis of aChemical Plant Defense Mechanism in Grasses, Frey, M. et al, 1997, Science277: 696-699; The Control of Maize Spikelet Meristem Fate by the APETALA2-Like Gene Indeterminate Spikelet 1, Chuck1 G., Meeley, R. B. e Hake1 S., 1998,Genes & Development 12: 1145-1154; A SecY Homologue is Required for theElaboration of the Chloroplast Thylakoid Membrane and for Normal ChloroplastGene Expression, Roy1 L. M. e Barkan, A., 1998, J. Cell Bioi 141: 1-11).

Seqüências de polinucleotídeos produzidas por meio de métodosde geração de diversidade ou métodos de recombinação de seqüênciasrecursivas ("RSR") (tais como alteração de DNA) são função da presenteinvenção. Métodos de mutação e recombinação utilizando os ácidos nucléicosdescritos no presente são função da presente invenção. Um método de acordocom a presente invenção inclui, por exemplo, a recombinação recursiva de umaou mais seqüências de nucleotídeos de acordo com a presente invençãoconforme descrito acima e abaixo com um ou mais nucleotídeos adicionais. Asetapas de recombinação são opcionalmente realizadas in vivo, ex vivo, in silicoou in vitro. Esta geração de diversidade ou recombinação de seqüênciasrecursivas produz pelo menos uma biblioteca de polinucleotídeos HATmodificados recombinantes. Os polipeptídeos codificados por membros destabiblioteca são incluídos na presente invenção.

Descrições de uma série de procedimentos geradores dediversidade, que incluem alterações de multigenes e métodos de geração deseqüências de ácidos nucléicos modificadas que codificam diversos domíniosenzimáticos, são encontradas nas publicações a seguir e nas referências alimencionadas: Soong1 N. et al (2000), Molecular Breeding of Viruses, Nat.Genet. 25 (4): 436-39; Stemmer et al (1999), MoIecuIarBreeding of Viruses forTargeting and Other Clinicai Properties, Tumor Targeting 4: 1-4; Ness et al(1999), DNA Shuffling of Subgenomic Sequences of Subtilisin, NatureBiotechnology 17: 893-896; Chang et al (1999), Evolution of a Cytokine UsingDNA Family Shuffling, Nature Biotechnology 17: 793-797; Minshull e Stemmer(1989), Protein Evolution by Molecular Breeding, Current Opinion in ChemicalBiology 3: 284-290; Christians et al (1999), Directed Evolution of ThymidineKinase for AZT Phosphorylation Using DNA Family Shuffling, NatureBiotechnology 17: 259-264; Crameri et al (1998), DNA Shuffling of a Family ofGenes from Diverse Species Aceelerates Direeted Evolution, Nature 391: 288-291; Crameri et al (1997), Molecular Evolution of an Arsenate DetoxificationPathway by DNA Shuffling, Nature Biotechnology 15: 436-438; Zhang et al(1997), Directed Evolution of an Effective Fucosidase from a Galactosidase byDNA Shuffling and Screening, Proc. Nati Acad. Sei. U. S. A. 94: 4504-4509;Patten et al (1997), Applications of DNA Shuffling to Pharmaceuticals andVaccines, Current Opinion in Biotechnology 8: 724-733; Crameri et al (1996),Construction and Evolution of Antibody-Phage Libraries by DNA-ShuffIing,Nature Medicine 2: 100-103; Crameri et al (1996), Improved Green FluorescentProtein by Molecular Evolution Using DNA Shuffling, Nature Biotechnology 14:315-319; Gates et al (1996), Affinity Selective Isolation of Ligands from PeptideLibraries Through Display on a Iac Repressor "Headpieace Dimerj', Journal ofMolecular Biology 255: 373-386; Stemmer (1996), Sexual PCR and AssemblyPCR, em The Encyclopedia of Molecular Biology, VCH Publishers, NovaIorque, págs. 447-457; Crameri e Stemmer (1995), Combinatorial MultipleCassette Mutagenesis Creates ali the Permutations of Mutant and WildtypeCassettes, BioTechniques 18: 194-195; Stemmer et al (1995), Single-StepAssembly of a Gene and Entire Plasmid from Large Numbers of Oligodeoxy-Ribonucleotides, Gene, 164: 49-53; Stemmer (1995), The Evolution ofMolecular Computation, Science 270: 1510; Stemmer (1995), SearchingSequence Space, Bio/Technology 13: 549-553; Stemmer (1994), RapidEvolution of a Protein in Vitro by DNA Shuffling, Nature 370: 389-391; eStemmer (1994), DNA Shuffling by Random Fragmentation and Reassembly: InVitro Recombination for Molecular Evolution, Proc. Natl. Acad. Sei. U. S. A. 91:10747-10751. Detalhes adicionais referentes a diversos métodos de geraçãode diversidade podem ser encontrados nas patentes norte-americanas,publicações PCT e publicações EPO a seguir: Patente Norte-Americana n°5.605.793 de Stemmer (25 de fevereiro de 1997), Methods for in VitroRecombination-, Patente Norte-Americana n° 5.811.238 de Stemmer et al (22 desetembro de 1998), Methods for Generating Polynucleotides Having DesiredCharacteristics by Iterative Seleetion and Recombination-, Patente Norte-Americana n° 5.830.721 de Stemmer et al (três de novembro de 1998), DNAMutagenesis by Random Fragmentation and Reassembly, Patente Norte-Americana n° 5.834.252 de Stemmer et al (dez de novembro de 1998), End-Complementary Polymerase Reaction-, Patente Norte-Americana n° 5.837.458de Minshull et al (17 de novembro de 1998), Methods and Compositions forCellular and Metabolie Engineering; WO 95/22625, Stemmer e Crameri,Mutagenesis by Random Fragmentation and Reassembly, WO 96/33207 deStemmer e Lipschutz, End Complementary Polymerase Chain Reaction-, WO97/20078 de Stemmer e Crameri, Methods for Generating PolynucleotidesHaving Desired Characteristics by Iterative Selection and Recombination-, WO97/35966 de Minshull e Stemmer, Methods and Compositions for Cellular andMetabolic Engineering; WO 99/41402 de Punnonen et al, Targeting of GenetieVaccine Vectors\ WO 99/41383 de Punnonen et al, Antigen LibraryImmunization-, WO 99/41369 de Punnonen et al, Genetic Vaccine VectorEngineering-, WO 99/41368 de Punnonen et al, Optimization ofImmunomodulatory Properties of Genetic Vaccines] EP 752008 de Stemmer eCrameri, DNA Mutagenesis by Random Fragmentation and Reassembly, EP0932670 de Stemmer, Evolving Cellular DNA Uptake by Recursive SequenceRecombination-, WO 99/23107 de Stemmer et al, Modification of Virus Tropismand Host Range by Viral Genome Shuffling: WO 99/21979 de Apt et al, HumanPapillomavirus Vectors; WO 98/31837 de dei Cardayre et al, Evolution of WholeCells and Organisms by Recursive Sequence Recombination; WO 98/27230 dePattern e Stemmer, Methods and Compositions for Polypeptide Engineering;WO 98/13487 de Stemmer et al, Methods for Optimization of Gene Therapy byReeursive Sequenee Shuffling and Seleetion; WO 00/00632, Methods forGenerating Highly Diverse Libraries; WO 00/09679, Methods for Obtaining inVitro Reeombined Polynucleotide Sequenee Banks and Resulting Sequenees,WO 98/42832 de Arnold et al, Reeombination of Polynucleotide SequeneesUsing Random or Defined Primers; WO 99/29902 de Arnold et al, Method forCreating Polynucleotide and Polypeptide Sequenees; WO 98/41653 de Vind,An in Vitro Method for Construetion ofa DNA Library·, WO 98/41622 de Borchertet al, Method for Construeting a Library Using DNA Shuffling] WO 98/42727 dePati e Zarling, Sequenee Alterations Using Homologous Reeombination; WO00/18906 de Patten et al, Shuffling of Codon-Altered Genes; WO 00/04190 dedei Cardayre et al, Evolution of Whole Cells and Organisms by ReeursiveRecombination-, WO 00/42561 de Crameri et al, Oligonucleotide MediatedNueleie Aeid Recombination-, WO 00/42559 de Selifonov e Stemmer, Methodsof Populating Data Struetures for Use in Evolutionary Simulations; WO00/42560 de Selifonov et al, Methods for Making Charaeter Strings,Polynucleotides & Polypeptides Having Desired Characteristics\ WO 01/23401de Welch et al, Use of Codon-Varied Oligonucleotide Synthesis for SynthetieShuffling-, e WO 01/64864, Single-Stranded Nueleie Aeid Template-MediatedRecombination and Nucleic Aeid Fragment Isolation de Affholter.

Certos pedidos norte-americanos fornecem detalhes adicionaisreferentes a vários métodos de geração de diversidade, que incluem Shufflingof Codon Altered Genes de Patten et al, depositado em 28 de setembro de1999 (USSN 09/407.800); Evolution of Whole Cells and Organisms byReeursive Sequenee Recombination, de dei Cardayre et al, depositado emquinze de julho de 1998 (USSN 09/166.188), e quinze de julho de 1999(Patente Norte-Americana n° 6.379.964); Oligonucleotide Mediated NucleicAcid Recombination de Crameri et al, depositado em 28 de setembro de 1999(Patente Norte-Americana n° 6.376.246); Oligonucleotide Mediated Nueleie- Aeid Reeombination de Crameri et al, depositado em 18 de janeiro de 2000(WO 00/42561); Use of Codon-Based Oligonucleotide Synthesis for SynthetieShuffling de Weleh et al, depositado em 28 de setembro de 1999 (PatenteNorte-Amerieana n° 6.436.675); Methods for Making Charaeter Stríngs,Polynucleotides & Polypeptides Having Desired Charaeteristies de Selifonov etal, depositado em 18 de janeiro de 2000 (WO 00/42560); Methods for MakingCharaeter Stríngs, Polynucleotides & Polypeptides Having DesiredCharaeteristies de Selifonov et al, depositado em 18 de julho de 2000 (USSN09/618.579); Methods of Populating Data Struetures for Use in EvolutionarySimulations de Selifonov e Stemmer (WO 00/42559), depositado em 18 dejaneiro de 2000; e Single-Stranded Nueleie Aeid Template-MediatedReeombination and Nueleie Aeid Fragment Isolation de Affholter (USSN60/186.482, depositado em dois de março de 2000). Podem também serutilizados métodos de recombinação sintética, nos quais oligonucleotídeoscorrespondentes a alvos de interesse são sintetizados e novamente reunidosem PCR ou reações de ligação que incluem oligonucleotídeos quecorrespondem a mais de um ácido nucléico parental, de forma a gerar novosácidos nucléicos recombinados. Oligonucleotídeos podem ser elaborados pormeio de métodos de adição de nucleotídeos padrão, ou podem ser elaborados,por exemplo, por meio de abordagens sintéticas trinucleotídeos. Detalhesreferentes a essas abordagens são encontrados nas referências indicadasacima, que incluem, por exemplo, WO 00/42561 de Crameri et al,Oligonucleotide Mediated Nueleie Aeid Recombination-, WO 01/23401 de Welchet al, Use of Codon-Varied Oligonucleotide Synthesis for Synthetie Shuffling·,WO 00/42560 de Selifonov et al, Methods for Making Character Strings,Polinucleotides and Polypeptides Having Desired Characteristics; e WO00/42559 de Selifonov e Stemmer, Methods of Populating Data Structures forUse in Evolutionary Simulations.

Podem ser efetuados métodos de recombinação in silico, nosquais algoritmos genéticos são utilizados em computador para recombinarconjuntos de seqüências que correspondem a ácidos nucléicos homólogos (oumesmo não homólogos). Os conjuntos de seqüências recombinadosresultantes são opcionalmente convertidos em ácidos nucléicos por meio desíntese de ácidos nucléicos, que correspondem às seqüências recombinadas,tal como em conjunto com métodos de reunião de genes por síntese deoligonucleotídeos. Esta abordagem pode gerar variantes aleatórias,parcialmente aleatórias ou projetadas. Muitos detalhes referentes àrecombinação in silico, que incluem o uso de algoritmos genéticos, operadoresgenéticos e similares em sistemas de computadores, combinados com ageração de ácidos nucléicos correspondentes (e/ou proteínas), bem comocombinações de ácidos nucléicos projetados e/ou proteínas (tal como combase em seleção de local por cruzamento), bem como métodos derecombinação projetada, pseudoaleatória ou aleatória, são descritos em WO00/42560 por Selifonov et al, Methods for Making Charaeter Strings,Polynucleotides and Polypeptides Having Desired Characteristies, e WO00/42559 de Selifonov e Stemmer, Methods of Populating Data Struetures forUse in Evolutionary Simulations. Extensos detalhes referentes a métodos derecombinação in silico são encontrados nesses pedidos. Esta metodologiageralmente é aplicável à presente invenção ao proporcionar recombinação deseqüências de ácido nucléico e/ou construções de fusão genética quecodificam proteínas envolvidas em diversos processos metabólicos (tais comoprocessos biossintéticos de carotenóide, processos biossintéticos de ectoína,processos biossintéticos de poli-hidroxialcanoato, processos biossintéticos depolicetídeos aromáticos e similares) in silico e/ou a geração de proteínas ouácidos nucléicos correspondentes.

Muitas das metodologias descritas acima para a geração depolinucleotídeos modificados geram grande quantidade de variantesdiversas de seqüência(s) parental(is). Em algumas realizações preferidasda presente invenção, o método de modificação (tal como alguma formade alteração) é utilizado para gerar biblioteca de variantes que éselecionada em seguida para polinucleotídeo modificado ou conjunto depolinucleotídeos modificados que codificam algum atributo funcionaldesejado, tal como maior atividade de HAT. Exemplos de atividadesenzimáticas que podem ser selecionadas incluem, mas sem limitar-se avelocidades catalíticas (convencionalmente caracterizadas em termos deconstantes cinéticas, tais como kcat e KM), especificidade de substrato esuscetibilidade à ativação ou inibição por substrato, produto ou outrasmoléculas (tais como inibidores ou ativadores) e a velocidade máxima dereação enzimática quando o local de união for saturado com substrato(Vmax).

Exemplos

A presente invenção é adicionalmente definida nos Exemplos aseguir, nos quais as partes e percentuais são em peso e os graus são Celsius1a menos que indicado em contrário. Dever-se-á compreender que estesExemplos, embora indiquem realizações preferidas da presente invenção, sãofornecidos unicamente como forma de ilustração. A partir da discussão acima edestes Exemplos, os técnicos no assunto podem determinar as característicasessenciais da presente invenção e, sem abandonar seu espírito e escopo,podem elaborar várias mudanças e modificações da presente invenção paraadaptá-la a vários usos e condições.Exemplo 1

Composição de Bibliotecas de cDNA: Isolamento ε Seqüenciamento deClones de cDNA

Foram preparadas bibliotecas de cDNA que representammRNAs de vários tecidos de milho. As características das bibliotecas sãodescritas na Tabela 1.

Bibliotecas de cDNA podem ser preparadas por meio de qualquerdentre diversos métodos disponíveis. Os cDNAs podem ser introduzidos, porexemplo, em vetores de plasmídeos, preparando-se em primeiro lugar asbibliotecas de cDNA em vetores Uni-ZAP® XR1 de acordo com o protocolo dofabricante (Stratagene Cloning Systems, La Jolla CA). As bibliotecas Uni-ZAP®XR são convertidas em bibliotecas de plasmídeos de acordo com o protocolofornecido pela Stratagene. Mediante conversão, os insertos de cDNA estarãocontidos no vetor de plasmídeo pBluescript. Além disso, os cDNAs podem serintroduzidos diretamente em vetores Bluescript Il SK(+) cortados previamente(Stratagene), utilizando T4 DNA Iigase (New England Biolabs), seguida portransfecção em células DH10B de acordo com o protocolo do fabricante(GIBCO BRL Products). Uma vez que os insertos de cDNA encontrem-se emvetores de plasmídeos, DNAs de plasmídeos são preparados a partir decolônias bacterianas tomadas aleatoriamente, contendo plasmídeos pBluescriptrecombinantes, ou as seqüências de cDNA do inserto são amplificadas pormeio de reação em cadeia de polimerase, utilizando primers específicos paraseqüências de vetores que ladeiam as seqüências de cDNA inseridas. DNAsde plasmídeos ou DNAs de insertos amplificados são seqüenciados emreações de seqüenciamento de primers de tintura para gerar seqüências decDNA parciais (marcas de seqüências expressas ou "ESTs"; vide Adams et al(1991), Science 252: 1651-1656). Os ESTs resultantes são analisadosutilizando seqüenciador fluorescente Perkin Elmer Modelo 377.Tabela 1

Bibliotecas de cDNA ε Clones que Contêm Seqüências Similares a NAR2de Milho

<table>table see original document page 54</column></row><table>

Exemplo 2

Identificação de Clones de cDNA

Clones de cDNA que codificam componentes associados aotransporte de nitrato foram identificados por meio da condução de BLAST(Ferramenta de Busca de Alinhamento Local Básico; Altschul et al (1993), J.Mol. Biol. 215: 403-410) e são exibidos na Tabela 1.

Os clones de cDNA codificadores de transportadores oucomponentes associados ao transporte de nitrato podem ser identificados pormeio da condução de buscas BLAST (Ferramenta de Busca de AlinhamentoLocal Básico; Altschul et al (1993), J. Mol. Biol. 215: 403-410) de similaridade aseqüências contidas no banco de dados "nr" do BLAST (que compreende todasas traduções de CDS GenBank não redundantes, seqüências derivadas doBanco de Dados de Proteínas Brookhaven de estrutura tridimensional, a últimapublicação principal do banco de dados de seqüências de proteínas SWISS-PROT, dos bancos de dados EMBL e DDBJ). As seqüências de cDNA obtidaspodem ter analisada sua similaridade com todas as seqüências de DNAdisponíveis ao público contidas no banco de dados "nr", utilizando o algoritmoBLASTN fornecido pelo Centro Nacional de Informações Biotecnológicas(NCBI). As seqüências de DNA podem ser traduzidas em todas as estruturasde leitura e ter sua similaridade comparada com todas as seqüências deproteínas disponíveis ao público, contidas no banco de dados "nr", utilizando oalgoritmo BLASTX (Gish e States (1993), Nat. Genet. 3: 266-272) fornecidopelo NCBI. Para melhor conveniência, o valor P (probabilidade) de observaçãode coincidência entre seqüência de cDNA e seqüência contida nos bancos dedados pesquisados meramente ao acaso, conforme calculado por meio deBLAST, é relatado no presente como valor "pLog", que representa o negativodo Iogaritmo do valor P relatado. Conseqüentemente, quanto maior o valorpLog, maior a probabilidade da seqüência de cDNA e do "encontrado" peloBLAST representarem proteínas homólogas.

Exemplo 3

Identificação ε Seqüenciamento de Transportadores de Nitrato com AltaAfinidade de Milho (HAT4 ε HAT5)

A fim de identificar homólogos de HATs, gene HAT público(número de acesso Genbank AY129953) foi utilizado para selecionar conjuntode genoma de milho MAGI versão 2.31 da Universidade do Estado de lowa.Clone parcial, MAGI 17514, que exibiu 85% de identidade em nível denucleotídeo e aparentemente é HAT anteriormente não identificado foiidentificado utilizando Blast no conjunto ISU MAGI. Esta seqüência foi utilizadapara selecionar o conjunto de dados Genbank GSS e alguns homólogosadicionais da seqüência de MAGI foram identificados; estes agregaram cercade 0,5 kb à seqüência. O conjunto de dados GSS consiste de seqüênciasestabelecidas em números de identificação geral: 33941728, 34245424,32105143, 34245411, 34082540 e 33992813. A tradução do conjunto cobriu cercade metade do gene, na extremidade 3'. Faltou completamente a metade 5' do gene.

A fim de isolar a seqüência de HAT4 de comprimento total, clonesde BAC de duas bibliotecas de BAC derivadas da linha congênita B73 de milhoforam selecionados utilizando PCR. As bibliotecas haviam sido anteriormenteconstruídas por meio de digestão parcial de DNA genômico e inseridas noslocais BamHI e EcoRI do pCUGI (Tomkins, J. P. et al, 2002, Construction andCharacterization of a Deep-Coverage Bacterial Artificial Chromosome Libraryfor Maize, Crop Science 42: 928-933) e pTARBAC (biblioteca pTARBAC2.1,Osoegawa, K. et al, Construction of New Maize, Bovine, Equine and ZebrafishBac Libraries, Plant and Animal Genome Conference Proceedings, 2001). Parafacilitar seleção com base em PCR, conjunto de 36 superconjuntos com quatrodimensões foi solicitado à Amplicon Express (Amplicon Express, 161 ONEEastgate Blvd Pullman, WA 99163). Cada superconjunto foi derivado após ocrescimento independente, isolamento e reunião de 4608 clones, mais de165.000 clones de BAC reunidos no total. Os superconjuntos foram submetidosa reações de PCR, seguidas por determinação mais-menos de fragmentos emeletroforese de gel de agarose. Primers de PCR foram projetados paraamplificar fragmento de 495 bp localizado a 289 bp abaixo no fluxo do códonde parada de homólogo de HAT localizado no conjunto Tigr ID AZM4_32787,que é idêntico às seqüências reunidas dos bancos de dados MAGI e GSSdescritos acima. Reações de PCR foram realizadas com 5 ng de DNA modeloem 10 μΙ de reação que incluiu 5 μΙ de mistura de polimerase Taq Hotstar(Qiagen) e 5 pmol dos primers frontal e reverso (SEQ ID N0 1 e SEQ ID N0 2,respectivamente). As condições de ciclo foram etapa de desnaturação inicial a95 0C por quinze minutos, seguida por 35 ciclos de 95 0C por trinta segundos,60 0C por trinta segundos e 72 0C por um minuto. Segunda rodada de PCR foirealizada em placas matriz que consistem de conjuntos combinatórios decomplexidade mais baixa derivados de clones representados em conjuntospositivos. Isso reduziu os positivos para clones específicos. Dois clones,bacc.pk139.d24 e bacc.pk142.b21, foram identificados e confirmados por meio deanálise de PCR. O clone bacc.pk139.d24 foi utilizado em trabalho subseqüente.

DNA de BAC do clone bacc.pk139.d24 foi isolado a partir decultivos de 250 ml de 2xYT+cloranfenicol utilizando método de Iise alcalinamodificada. As células foram colhidas por meio de centrifugação e novamentesuspensas em 20 ml de 10 mM de EDTA, Iisadas em seguida por meio desuave adição de 40 ml de 0,2 N NaOH/1% SDS e neutralizadas com 30 ml de 3M acetato de potássio frio (pH 4,8). Fragmentos celulares foram removidos pormeio de centrifugação a 4 0C por quinze minutos a 15000 xg, seguida porfiltragem por meio de Miracloth. DNA em sobrenadante foi precipitado com 0,7volumes de isopropanol e novamente suspenso em 9 ml de 50 mM de Tris/50mM de EDTA, misturado com 4,5 ml de 7,5 M de acetato de potássio, colocadoa -70 0C1 descongelado e centrifugado por vinte minutos a 3500 xg. Osobrenadante foi decantado, precipitado com etanol e novamente suspenso em0,7 ml de 50 mM de Tris/50 mM de EDTA. RNase A livre de DNases foiadicionada até concentração final de 150 pg/ml e incubada por uma hora a 370C, seguida por extração com fenolxlorofórmio e precipitação de etanol. DNAfinal foi novamente suspenso em total de 400 μΙ de água livre de nucleaseestéril. O tamanho, quantidade e qualidade do inserto de DNA foramdeterminados por meio de Eletroforese de Gel de Campo Pulsado utilizandomapeador CHEF-III (Bio-Rad). Para seqüenciamento de extremidades de BACconfirmatórias, foram utilizados os primers T7 (SEQ ID N0 3) e SP6 (SEQ ID N04), empregando condições de seqüenciamento descritas abaixo.

A estratégia geral de obtenção de informações de seqüênciascontíguas de fita dupla junto com o gene HAT4 foi partir da seqüência "inicial"conhecida definida pelos primers de identificação de PCR, descritaanteriormente. DNA de BAC bacc.pk139.d24 foi utilizado como modelo. Oseqüenciamento foi realizado em seqüenciador capilar ABI3730 de acordo comos protocolos do fabricante. As reações de seqüenciamento consistiram de 2 μlde mistura BigDye V3.1 Terminator (Applied Biosystems), 2 μl de tampão dediluição (600 mM de Tris HCI, pH 9,0, 15 mM de MgCI2), 20 pmol de primer ecerca de 1 μg de DNA modelo em volume final de reação de 20 μl. Ascondições de ciclo foram desnaturação inicial a 95°C por cinco minutos,seguida por 99 ciclos de 95°C por trinta segundos, 58°C por trinta segundos e64°C por quatro minutos. Algumas regiões de difícil leitura necessitaram sernovamente seqüenciadas utilizando condições especiais de ciclo e reação. Oexcesso de término de tintura foi removido por meio de precipitação cometanol. Avaliação de traços, chamada de bases e reunião foram baseadas emsoftware Phred/Phrap (Ewing et al (1998), Genome Res. 8: 186-194; Ewing etal (1998), Genome Res. 8: 175-185). Consed (Gordon et al (1998), GenomeRes. 8: 195-202) foi utilizado para análise de conjunto. Após cada etapa deandamento da seqüência, primers foram projetados nas extremidades, evitandoregiões de alta homologia para outros genes e repetições de DNA. A busca dehomologia foi realizada utilizando o programa BLAST (Ferramenta de Busca deAlinhamento Local Básico; Altschul et al (1993), J. Moi Biol. 215: 403-410)contra gss, TIGR 4.0, não redundante, EST e bancos de dados de proteínas(Altschul et al, 1990). O vetor NTI foi utilizado para projeto de primers e osprimers foram sintetizados comercialmente pela MWG Biotech. Primers (SEQID N0 5 até SEQ ID N0 33) foram projetados, testados e utilizados para cobrir aregião que inclui o gene HAT. SEQ ID N0 34 descreve a seqüência genômicaque contém o gene HAT 4. SEQ ID N0 35 e 36 descrevem a seqüência denucleotídeos de codificação e de aminoácidos do HAT4 de milho,respectivamente.

SEQ ID N0 37 e 38 exibem as supostas seqüências promotorasde 2014 bp e 1014 bp do gene HAT4.A família de HAT-5 foi identificada por meio de homologia de Blastpara os HATs públicos. Um clone 3' cco1n.pk072.i13 apresentou homologia paraMAGI_56254, que aparentemente representa a seqüência completa. O conjuntode TIGR AZM4_2103 correspondeu bem ao clone de MAGI. Bancos de dados quecontêm bibliotecas induzidas por nitrogênio sofreram novamente Blast utilizandoeste clone e foi identificado o clone cfp4n.pk008.p6. Este clone foi seqüenciado econtém a seqüência de gene HAT5 completa (SEQ ID N0 91 e 92).

Exemplo 4

Identificação ε Seqüenciamento de Transportador de Nitrato com AltaAfinidade de Milho Adicional (HAT7)

Gene HAT público (HAT1, número de acesso GenbankAY129953) foi utilizado para pesquisa com Blast, seqüências de pesquisagenômica (GSS) de milho Genbank e conjuntos genômicos de milho (MAGI daUniversidade do Estado de Iowa e Tigr), para tentar identificar parálogos deAY129953. Junto com o gene HAT4 (Exemplo 3), havia outros homólogos maisdistantes, que incluem MAGI_65216 que correspondeu a AZM4_79242, quecontinha pouco mais informação de seqüências que MAGI_65216). Nenhumdestes dois clones continha metionina inicial. Coincidência adicional paraAZM4 79246 exibiu percentual de identidade similar em comparação comAY129953. AZM4_79246 codificou metionina inicial no nucleotídeo 2264-2266e cerca de 110 aminoácidos de seqüência de codificação. Exame adicionaldemonstrou que estes dois conjuntos compartilharam clones coincidentes,OGUKX93 e OGUCS47, da biblioteca filtrada por metilação de Tigr.Considerou-se, portanto, que AZM4_79242 e AZM4_79246 codificam o mesmogene, mas não possuem sobreposição de seqüências.

A fim de recuperar a seqüência de comprimento total, realizou-sePCR utilizando dois primers reversos diferentes e dois frontais diferentes (SEQID N0 39, 40 e 41, 42, respectivamente) com extensões T3 (SEQ ID N0 43) e T7(SEQ ID N0 44 na extremidade 5' e 3', respectivamente). PCR HotStart1 comtemperatura de combinação de 58 0C1 foi realizada utilizando DNA de oitolinhas congênitas de milho (B73, Co159, GT119, Mo17, T218, Oh43 e W23)como modelos. Todos os 32 produtos de reação de PCR foram conduzidossobre gel 1x TBE de agarose, extirpados, limpos e seqüenciados emSeqüenciador Capilar 3100 ABI utilizando métodos conhecidos dos técnicoscomuns no assunto. As seqüências foram alinhadas e as informações deseqüência faltantes foram recuperadas. A seqüência de nucleotídeos completado gene HAT7 é exibida em SEQ ID N0 45. SEQ ID N0 46 e 47 descrevem assupostas seqüências promotoras 2263 bp e 1263 bp do gene HAT7 e SEQ IDN0 48 e 49 descrevem a seqüência de nucleotídeos de codificação e deaminoácidos do HAT7 de milho, respectivamente.

Exemplo 5

Caracterização de Polipeptídeos que Codificam Transportador de Nitratocom Alta Afinidade

Os dados da Tabela 2 representam cálculo do percentual deidentidade das seqüências de aminoácidos descritas em SEQ ID N0 36 e 49 e dasseqüências de Oryza sativa (Identificador Geral NCBI n° 34913806 e 50904699).

Tabela 2

Percentual de Identidade de Seqüências de Aminoácidos Deduzidas dasSeqüências de Nucleotídeos de Clones de cDNA que CodificamPolipeptídeos Homólogos a Transportador de Nitrato com Alta Afinidade

<table>table see original document page 60</column></row><table>

Alinhamentos seqüenciais e cálculos de identidade percentualforam realizados utilizando o programa Megalign da suíte de computaçãobioinformática LASERGENE (DNASTAR Inc., Madison Wl). O alinhamentomúltiplo das seqüências foi realizado utilizando o método de alinhamentoClustal (Higgins e Sharp (1989), CABIOS. 5: 151-153) com os parâmetrospadrão (PENALIDADE DO INTERVALO = 10, PENALIDADE DOCOMPRIMENTO DO INTERVALO = 10). Os parâmetros padrão paraalinhamentos em pares utilizando o método Clustal foram COMPRIMENTO DEPALAVRA = 1, PENALIDADE DO INTERVALO = 3, VISUALIZAÇÃO DOMELHOR RESULTADO = 5 e ESPAÇAMENTO EM DIAGONAIS = 5. Alinhamentosde seqüências e avaliações e probabilidades de BLAST indicam que os fragmentosde ácidos nucléicos que compreendem os clones de cDNA do presente codificamtransportadores de nitrogênio com alta afinidade de milho.

Exemplo 6

Identificação ε Seqüenciamento de Genes Relativos ao Transporte deNitrogênio de Milho fNAR2-1 e NAR2-2)

Exame de coincidências de Blast da biblioteca de raiz de milhocnrlc, descrita no Exemplo 1 e na Tabela 2, exibiu uma série de genesrelativos ao transporte de nitrogênio. Coincidências de Blast foram pesquisadascom palavras chave tais como nitrato, nitrogênio e transportador. Algumasdestas foram homólogas ao número de acesso NCBI CAC36942, supostocomponente de transportador de nitrato com alta afinidade (gene NAR2).Pesquisa TbIastN de ESTs de milho, utilizando a seqüência de CAC36942como pesquisa, produziu uma série de coincidências significativas dediferentes bibliotecas de milho. O clone mais 5' foi identificado por meio dealinhamento da pesquisa de comprimento total e as coincidências de Blast.Clone da biblioteca de cnrlc (cnr1c.pk003.m9.f) exibiu metionina que seencontrava na mesma região da metionina inicial de CAC36942. Este clonetambém exibiu códon de parada em quadro acima no fluxo da metionina. Esteclone foi submetido a seqüenciamento de inserto total (FIS) padrão e continhaos 971 bp do NAR2.1, cobrindo os nucleotídeos 591 a 1561 de SEQ ID N0 53.SEQ ID N0 53 exibe a seqüência de 1561 bp do gene NAR2.1, que foi reunida apartir das informações de seqüências obtidas a partir do clonecnr1c.pk003.m9.f:fis e da seqüência de Tigr AZM4_81138. SEQ ID N0 54 e 55exibem a seqüência de nucleotídeos de codificação e de aminoácidos do geneNAR2.1, respectivamente. SEQ ID N0 56 exibe 756 bp do suposto promotor deNAR2.1. O uso de CAC36942 como pesquisa também exibiu diferentehomólogo de NAR2, cbn2.pk0042.g4. Este clone também continha metioninainicial mas, devido à qualidade da seqüência de EST1 a homologia paraCAC36942 foi curta. Versão completa (clone de Tigr AZM4_1475) destemembro da família foi identificada por meio de pesquisa do conjunto genômicode milho Tigr utilizando cbn2.pk0042.g4 como pesquisa. SEQ ID N0 57 e 58exibem a seqüência de nucleotídeos de codificação e de aminoácidos doNAR2.2 (clone Tigr AZM4_1475), respectivamente.

Isolamento do promotor NAR2.1

As informações de seqüências sobre o promotor NAR2.1 foramestendidas mais acima no fluxo realizando-se andamento de DNA GenomeWalker® (BD BioSciences). Este método emprega PCR para facilitar aclonagem de seqüências de DNA genômicas desconhecidas adjacentes aseqüência conhecida. Em primeiro lugar, conjuntos de DNA genômicodesconhecido foram digeridos com diferentes enzimas de restrição que deixamextremidades obtusas. Cada conjunto foi ligado a adaptadores para criarbibliotecas Genome Walker®. Foram obtidas oito bibliotecas de HG11 de milhodiferentes. Estas bibliotecas foram digeridas com as enzimas de restrição aseguir: Stul, EcoRV, Pmll, Pvull, Seal, Dral, Smal e Pmel.

Em seguida, foram realizadas duas rodadas de amplificação porPCR reunidas por biblioteca. Para a primeira rodada, foram utilizados o primeradaptador externo (AP1, equipado com kit) e o primer externo específico deNar2.1 (SEQ ID N0 59).

PCR foi realizada utilizando a Mistura de Polimerase GenômicaAdvantage® GC (BD Biosciences) em 50 μΙ de reação contendo 1 μΙ debiblioteca de DNA1 0,5 μΙ de cada primer (10 μΜ), 4 μΙ de dNTPs (2,5 mM), 2,2μΙ de Mg(OAc)2, 10 μΙ de 5x Tampão de Reação de PCR Genômico GC, 10 μΙde GC-MeIt (5 M), 20,8 μΙ de ddH20 e 1 μΙ de Polimerase Genômica AdvantageGC. As condições de ciclização foram as seguintes: sete ciclos dedesnaturação a 94 0C por 25 segundos e combinação/extensão a 72 0C porseis minutos seguida por 32 ciclos de desnaturação a 94 0C por 25 segundos ecombinação/extensão a 67 0C por seis minutos coberta porcombinação/extensão a 67 0C por sete minutos.

O produto de PCR primário foi diluído em seguida a 1:50 e 1 μΙ foiservido como modelo para a segunda rodada de PCR que utilizou a mesmaconfiguração de PCR da primeira rodada. Os primers da segunda rodada foramo primer adaptador interno (AP2, fornecido com o kit) e o primer internoespecífico de Nar2.1 (SEQ ID N0 60). As condições de ciclização para asegunda rodada foram as seguintes: cinco ciclos de desnaturação a 94 0C por 25segundos e combinação/extensão a 72 0C por seis minutos seguida por 25 ciclos dedesnaturação a 94 0C por 25 segundos e combinação/extensão a 67 0C por seisminutos coberta por combinação/extensão a 67 0C por sete minutos.

Produto de PCR importante (cerca de 3 kb) foi observado nabiblioteca de Stul. Esta faixa foi cortada do gel e purificada utilizando o Kit deExtração de Gel Quiaquick (Qiagen) e ligada a Vetor pGEM®-T Easy(Promega). A reação de ligação de 20 μΙ foi a seguinte: 10 μΙ 2X Tampão deLigação Rápida, 1 μΙ de Vetor pGEM®-T Easy (50 ng), 1 μΙ de T4 DNA Iigase (2unidades Weiss/μΙ) e 8 μΙ de DNA de inserto (13 ng/μΙ). A reação foi incubada a4 'C por uma noite.O produto de ligação foi transformado em células competentesDH10B de Máxima Eficiência (Invitrogen). Um microlitro de ligado foiadicionado a 20 μl de células e colocado sobre gelo por trinta minutos. Ascélulas sofreram choque de calor a 42°C por 45 segundos e foram novamentecolocadas sobre gelo por dois minutos. As células foram adicionadas a 1 ml de SOCe colocadas sobre agitador a 250 rpm por uma hora a 37°C. Em seguida, 100 μl decélulas foram colocados em placas sobre meios LB com ampicilina, IPTG e X-Galpara permitir seleção azul/branca. Somente uma colônia branca foi obtida.

DNA de plasmídeo foi purificado utilizando o Mini Kit Plasmid(Qiagen). O inserto de plasmídeo que representa a região promotora acima nofluxo de NAR2 foi seqüenciado utilizando primers padrão (SP6 e T7) e primersespecíficos (SEQ ID N0 61, 62, 63 e 64). SEQ ID N0 65 exibe a seqüência dosuposto promotor de NAR 2.1 de 2917 bp adicional.

A seqüência do gene NAR2.1 completo é exibida em SEQ ID N0 66.

Exemplo 7

Padrão de Expressão dos Polipeptídeos de Acordo com a PresenteInvenção

O padrão de expressão de transportadores de nitrato com altaafinidade (HAT) e outros polipeptídeos (NAR) necessários para transporte denitrato com alta afinidade foi analisado por meio de Lynx MPSS, Brenner et al(2000), Proc. Natl. Acad. Sei. U. S. A. 97: 1665-70.

Os padrões de expressão de genes NAR2.1 e HAT, são similaresao longo de mais de duzentas bibliotecas estudadas por meio de Lynx MPSS(Brenner et al (2000), Proc. Natl. Acad. Sei. U. S. A. 97: 1665-70). Eles sãoambos expressos somente no cilindro cortical do tecido de raiz e são induzidosde forma similar por nitrato, o que indica que os produtos de polipeptídeodestes dois genes formam complexo funcional para transporte de nitrato emraízes de milho.Expressão específica de tecidos de NAR2.1 ε HAT-1 em milho:

das 210 bibliotecas de diferentes tecidos que englobam a plantade milho inteira, NAR2.1 e HAT-1 são expressos somente nas bibliotecas deraízes. Isso indica a função específica de raízes para cada um destes genes.

Análise de expressão de NAR2.1 e HAT-1 em tecidos de milho.Foi calculada a média das abundâncias de marca de MPSS ao longo dediferentes bibliotecas de tecidos. A quantidade de bibliotecas para cada tecidofoi: antera, 3; espiga, 15; semente, 44; folha, 39; pólen, 1; raiz, 36; seda, 9;haste, 19; e cabelo, 14.

Indução da absorção de nitrato ε localização em raízes de milhodentre as bibliotecas de raízes derivadas de linhagem congênitaA63, a expressão de NAR2.1 e HAT-1 é induzida de forma similar por nitrato.

Raízes de milho de mudas etioladas obtidas sete dias após ocultivo em rolos de papel em água foram colhidas e submetidas a diferentestratamentos em paralelo. As raízes recém colhidas foram mantidas sobre gelocomo controles. As raízes foram incubadas em solução aerada que contémdiferentes nutrientes por diferentes períodos de tempo e rapidamente congeladasem N líquido e armazenadas a -80 0C até o uso para análises de expressão ouguardadas entre duas camadas de papel toalha úmido em gelo para manipulaçãoadicional. Uma batelada de raízes que havia sido tratada por quatro horas emnitrato foi dissecada manualmente em cilindro cortical e monólito.

Reação de NAR2.1 e expressão de HAT 1 a tratamentos comdiferentes nutrientes. As raízes foram tratadas por meia hora ou quatro horasem meio que contém 1 mM de nitrato (0,5 mM de KNO3 e 0,25 mM deCa(NO3)2) ou 1 mM de cloreto (0,5 mM de KCI e 0,25 mM de CaCI2). Bateladade raízes tratada por quatro horas com nitrato foi separada em cilindro cortical

e monólito e submetida a MPSS.

Os genes NAR2.1 e HAT1 de milho exibem reação similar anitrato (N) no meio de incubação que aumenta ao longo do tempo emcomparação com as raízes controle paralelas incubadas em solução de cloreto.Além disso, esses dois genes são localizados quase exclusivamente na mangacortical e não no monólito. Sua reação similar a nitrato e sua localizaçãoindicam fortemente que os produtos de proteína desses genes realizamcomplexo de transporte de nitrato funcional em raízes de milho.

Regulagem oposta da expressão de NAR2.1 em linhagens de milho com altoteor de proteína de illinois (IHP) e baixo teor de proteína de illinois (ILP)IHP e ILP são dois conjuntos de linhagens que são derivadas depopulação de milho após cerca de cem anos de seleção divergente paraproteína de grão nas direções de alto e baixo teor de proteína no grão,respectivamente (Uribelarrea et al, 2004). Embora os grãos IHP contenhammais de 20% de proteína, os de ILP contêm menos de 5%. As raízes destasduas linhagens foram submetidas a Lynx MPSS após vários tratamentos.

As raízes foram mantidas em solução de nitrato por todo o tempo,permaneceram sem nitrato por duas horas ou foram colocadas em solução denitrato após duas horas sem ele. Embora NAR2.1 em IHP reagisse atratamento com nitrato como A63, ILP exibiu reação oposta. Dado o nível deexpressão deste gene em ILP em raízes com ausência de nitrato que é similarao de raízes de IHP mantidas em nitrato, estes resultados sugerem queexistem mecanismos que reagem a nitrato nas duas direções em milho. Omecanismo de reação positiva, entretanto, aparentemente foi selecionadoconforme indicado por meio de reação similar entre IHP e A63, linhagemcongênita com teor normal de proteína do grão de cerca de 10%.

Apenas IHP continha a marca para seqüência HAT1 e exibiupadrão de expressão similar a NAR2.1, gerando suporte adicional à sugestãomencionada acima de que NAR2.1 e HAT1 formam complexo funcional emraízes de milho.Expressão de outros genes HAT em A63

HAT 4G foi expresso em mais de 10 ppm somente em quatrobibliotecas, todas derivadas do tecido de raiz. Desta forma, aparentemente estegene é específico de raiz. HAT 7 é expresso em mudas resfriadas e trêsbibliotecas de folhas, o que sugere que este gene pode codificar proteína paraabsorção de nitrato do apoplasta xylem para as células de folhas. Espera-seque as seqüências de HAT de acordo com o presente pedido formem complexode transporte de nitrato funcional com seqüência de NAR.

Exemplo 8

Confirmação de Função dos Transportadores de Nitrato com Alta

Afinidade ε Polipeptídeos Necessários para Transporte de Nitrato comAlta Afinidade Utilizando a População Mutante TUSC

A seqüência genômica completa para o local transportador denitrato com alta afinidade pode ser utilizada para projetar primers para seleçãoem busca de mutantes com inserção de Mu na população de TUSC (PatenteNorte-Americana n° 5.962.764, emitida em cinco de outubro de 1999). Apopulação de TUSC reunida pode ser selecionada com primers específicos degenes. Alelos dos transportadores de nitrato com alta afinidade de milho epolipeptídeos necessários para transporte de nitrato com alta afinidade podemser recuperados desta seleção e caracterizados. Além disso, a função dasseqüências do presente pedido pode ser confirmada por estudos decomplementação.

Exemplo 9

Expressão de Construções de DNA Recombinante em CélulasMonocotiledóneas

Pode ser elaborada construção de DNA recombinante quecompreende cDNA codificador dos polipeptídeos do presente em orientaçãocom sentido com relação ao promotor zeina de 27 kD de milho, que estálocalizado a 5' do fragmento de cDNA, e à extremidade 3' zeina de 10 kD queestá localizada a 3' do fragmento de cDNA. O fragmento de cDNA deste genepode ser gerado por meio de reação em cadeia de polimerase (PCR) do clonede cDNA utilizando primers de oligonucleotídeos apropriados. Os locais declonagem (Ncol ou Smal) podem ser incorporados aos oligonucleotídeos paraproporcionar orientação adequada do fragmento de DNA quando inserido novetor digerido pML103, conforme descrito abaixo. Realiza-se em seguidaamplificação em PCR padrão. O DNA amplificado é digerido em seguida comas enzimas de restrição Ncol e Smal e fracionado sobre gel de agarose. A faixaapropriada pode ser isolada do gel e combinada com fragmento Ncol-Smal de4,9 kb do plasmídeo pML103. O plasmídeo pML103 foi depositado com basenos termos do Tratado de Budapeste na ATCC (Coleção Norte-Americana deCultivo de Tipos, 10801 University Blvd., Manassas VA, 20110-2209) e possuio número de acesso ATCC 97366. O segmento de DNA de pML103 contémfragmento promotor de Sall-Ncol de 1,05 kb do gene zeina de 27 kD de milho efragmento Smal-Sall de 0,96 kb da extremidade 3' do gene zeina de 10 kD demilho no vetor pGem9Zf(+) (Promega). O DNA de inserto e o vetor podem serligados a 15 cC por uma noite, essencialmente conforme descrito (Maniatis). ODNA ligado pode ser utilizado em seguida para transformar XLI-BIue de E. coli(XL-1 Blue® de Epicurian colr, Stratagene). Transformadores bacterianospodem ser selecionados por meio de digestão de enzimas de restrição de DNAde plasmídeo e análise de seqüências de nucleotídeos limitada, utilizando ométodo de término de cadeia dideóxi (Kit de Seqüenciamento de DNASequenase®; U. S. Biochemical). A construção de plasmídeo resultantecompreenderia construção de DNA codificadora, na direção 5' a 3', do promotorzeina de 27 kD de milho, fragmento de cDNA codificador dos polipeptídeos dopresente, e da região 3' zeina de 10 kD.

A construção de DNA recombinante descrita acima pode serintroduzida em seguida em células de milho por meio do procedimento aseguir. Embriões de milho imaturos podem ser dissecados a partir de cariopsesem desenvolvimento derivadas de cruzamentos das linhagens de milhocongênitas H99 e LH132. Os embriões são isolados em dez a onze dias após apolinização, quando estão com 1,0 a 1,5 mm de comprimento. Em seguida, osembriões são colocados com o lado do eixo voltado para baixo e em contatocom meio N6 solidificado com agarose (Chu et al (1975), Sei. Sin. Peking 18:659-668). Os embriões são mantidos no escuro a 27°C. O calo embriogênicoesboroadiço que consiste de massas não-diferenciadas de células comembrióides e proembrióides somáticos cultivados sobre estruturas desuspensão prolifera-se a partir do escutelo desses embriões imaturos. O caloembriogênico isolado a partir do explante primário pode ser cultivado sobremeio N6 e subeultivado sobre esse meio a cada duas a três semanas.

O plasmídeo, p35S/Ac (obtido por meio do Dr. Peter Eckes,Hoechst Ag, Frankfurt, Alemanha), pode ser utilizado em experimentos detransformação, a fim de proporcionar marcador selecionável. Este plasmídeocontém o gene Pat (vide Patente Européia n° 0.242.236), que codificafosfinotricin acetil transferase (PAT). A enzima PAT oferece resistência ainibidores de glutamino sintetase herbicidas, tais como fosfinotricin. O gene patem p35S/Ac encontra-se sob o controle do promotor 35S do Vírus Mosaico daCouve-Flor (Odell et al (1985), Nature 313: 810-812) e da região 3' do genenopalino sintase do T-DNA do plasmídeo Ti de Agrobacterium tumefaciens.

O método de bombardeamento de partículas (Klein et al (1987),Nature 327: 70-73) pode ser utilizado para a transferência de genes para ascélulas de cultivo de calo. De acordo com este método, partículas de ouro (comdiâmetro de 1 pm) são revestidas com DNA utilizando a técnica a seguir. Dezpg de DNAs de plasmídeo são adicionados a 50 μΙ de suspensão de partículasde ouro (60 mg por ml). São adicionados cloreto de cálcio (50 μΙ de umasolução de 2,5 Μ) e base livre de espermidina (20 μΙ de solução de 1,0 M) àspartículas. A suspensão é turbilhonada durante a adição dessas soluções.Após dez minutos, os tubos são rapidamente centrifugados (5 seg a 15.000rpm) e o sobrenadante é removido. As partículas são novamente suspensas em 200 μΙ de etanol absoluto, novamente centrifugadas e o sobrenadante éremovido. O enxágüe em etanol é realizado novamente e as partículas sãonovamente suspensas em volume final de 30 μΙ de etanol. Uma parcela (5 μΙ)das partículas de ouro revestidas com DNA pode ser colocada no centro dedisco suspenso Kapton® (Bio-Rad Labs). As partículas são aceleradas emseguida no tecido de milho com Biolistic® PDS-1000/He (Bio-Rad Instruments,Hercules CA), utilizando pressão de hélio de 1000 psi, distância de lacuna de0,5 cm e distância aérea de 1,0 cm.

Para o bombardeamento, o tecido embriogênico é colocado sobrepapel-filtro sobre meio N6 solidificado com agarose. O tecido é disposto naforma de tecido fino e cobriu área circular com cerca de 5 cm de diâmetro. Aplaca petri que contém o tecido pode ser colocada na câmara do PDS-1000/Hea cerca de 8 cm da tela de vedação. O ar da câmara é evacuado em seguidaaté vácuo de 711 milímetros de Hg. O macroveículo é acelerado com onda dechoque de hélio, utilizando membrana de ruptura que se rompe quando apressão de He no tubo de choque atingir 1000 psi.

Sete dias após o bombardeamento, o tecido pode ser transferidopara meio N6 que contenha glufosinato (2 mg por litro) e que não contenhacaseína ou prolina. O tecido continua a crescer lentamente sobre este meio.Após duas semanas adicionais, o tecido pode ser transferido para meio N6novo contendo glufosinato. Após seis semanas, podem ser identificadas áreasde cerca de 1 cm de diâmetro de calos em crescimento ativo sobre algumasdas placas que contêm o meio suplementado por glufosinato. Estes calospodem continuar a crescer quando subcultivados sobre o meio seletivo.As plantas podem ser regeneradas a partir do calo transgênico,transferindo-se primeiramente conjuntos de tecido para meio N6 suplementadocom 0,2 mg de 2,4-D por litro. Após duas semanas, o tecido pode ser transferidopara meio de regeneração (Fromm et al (1990), Bio/Technology 8: 833-839).

Exemplo 10

Expressão de Construções de DNA Recombinante em CélulasDicotiledôneas

Conjunto de expressão específico de sementes composto dopromotor e término de transcrição do gene codificador da sub-unidade α dafaseolina de proteína de armazenagem de sementes do feijão Phaseolusvulgaris (Doyle et al (1986), J. Biol. Chem. 261: 9228-9238) pode ser utilizadopara expressão dos polipeptídeos do presente em soja transformada. Oconjunto de faseolina inclui cerca de 500 nucleotídeos acima (5') do códon deinício de tradução e cerca de 1650 nucleotídeos abaixo (3') do códon de parada detradução de faseolina. Entre as regiões 5' e 3', encontram-se os locais deendonuclease de restrição exclusivos Nco I (que inclui o códon de início de traduçãoATG), Sma I, Kpn I e Xba I. Todo o conjunto é ladeado por locais Hind III.

O fragmento de cDNA deste gene pode ser gerado por meio dereação em cadeia de polimerase (PCR) do clone de cDNA, utilizando primersde oligonucleotídeos apropriados. Os locais de clonagem podem serincorporados aos oligonucleotídeos para proporcionar orientação adequada dofragmento de DNA quando inserido no vetor de expressão. Realiza-se entãoamplificação conforme descrito acima e o fragmento isolado é inserido em vetorpUC18 que conduz o conjunto de expressão da semente.

Embriões de soja podem ser transformados em seguida com ovetor de expressão que compreende seqüências codificadoras dospolipeptídeos do presente. Para induzir embriões somáticos, cotilédones com 3a 5 mm de comprimento dissecados a partir de sementes imaturas eesterilizadas na superfície do cultivo de soja A2872 podem ser cultivados na luzou no escuro a 26°C sobre meio agar apropriado por seis a dez semanas.Embriões somáticos que produzem embriões secundários são extirpadosem seguida e colocados em meio líquido apropriado. Após a seleçãorepetida de conjuntos de embriões somáticos que se multiplicaram naforma de embriões precoces em estágio globular, as suspensões sãomantidas conforme descrito abaixo.

Cultivos de suspensão embriogênica de soja podem ser mantidosem 35 ml de meios líquidos em agitador giratório, 150 rpm, a 26°C com luzes fluorescentes em programa de 16:8 horas de dia/noite. Os cultivos sãosubcultivados a cada duas semanas, por meio da inoculação de cerca de 35mg de tecido em 35 ml de meio líquido.

Cultivos de suspensão embriogênica de soja podem sertransformados em seguida por meio do método de bombardeamento comdisparadores de partículas (Klein et al (1987), Nature (London) 327: 70-73,Patente Norte-Americana n° 4.945.050). Pode ser utilizado instrumento DuPontBiolistic® PDS1000/HE (retroajuste de hélio) para estas transformações.

Gene marcador selecionável que pode ser utilizado parapossibilitar a transformação de soja é construção de DNA recombinantecomposta do promotor 35S de Vírus Mosaico da Couve-Flor (Odell et al (1985),Nature 313: 810-812), o gene higromicino fosfotransferase do plasmídeopJR225 (de E. co//; Gritz et al (1983), Gene 25: 179-188) e a região 3' do genenopalino sintase do T-DNA do plasmídeo de Ti de Agrobacterium tumefaciens.O conjunto de expressão de sementes que compreende a região 5' defaseolina, o fragmento codificador dos polipeptídeos do presente e a região 3'de faseolina podem ser isolados na forma de fragmento de restrição. Estefragmento pode ser inserido em seguida em local de restrição exclusivo dovetor que conduz o gene marcador.A 50 μl de suspensão de 60 mg/ml de partículas de ouro de 1 μm,adiciona-se (nesta ordem): 5 μΙ de DNA (1 pg/μl), 20 μl de espermidina (0,1 M)e 50 μl de CaCl2 (2,5 Μ). A preparação de partículas é agitada em seguida portrês minutos, centrifugada em microcentrifugador por dez segundos e osobrenadante foi removido. As partículas revestidas com DNA são lavadas emseguida por uma vez em 400 μl de etanol a 70% e novamente suspensas em40 μl de etanol anidro. A suspensão de DNA/partículas pode ser sonicada portrês vezes por um segundo cada. Cinco μl das partículas de ouro revestidascom DNA são carregados em seguida sobre cada disco macro veículo.

Cerca de 300 a 400 mg de cultivo em suspensão com duassemanas de idade são colocados em placa petri de 60 χ 15 mm vazio e olíquido residual é removido do tecido com pipeta. Para cada experimento detransformação, são normalmente bombardeadas cerca de cinco a dez placasde tecido. A pressão de ruptura da membrana é ajustada a 1100 psi e acâmara é evacuada até vácuo de 711 milímetros de mercúrio. O tecido écolocado a cerca de 8,9 cm de distância da tela retentora e bombardeado portrês vezes. Em seguida ao bombardeamento, o tecido pode ser dividido aomeio, colocado de volta no líquido e cultivado conforme descrito acima.

Cinco a sete dias após o bombardeamento, o meio líquido podeser substituído por meio novo e, de onze a doze dias após o bombardeamento,por meios novos contendo 50 mg/ml de higromicina. Este meio seletivo podeser renovado semanalmente. Sete a oito semanas após o bombardeamento,pode-se observar tecido verde transformado crescendo a partir de conjuntosembriogênicos necróticos não transformados. O tecido verde isolado éremovido e inoculado em frascos individuais para gerar novos cultivos desuspensão embriogênica transformados e propagados de forma clonal. Cadanova linhagem pode ser tratada como evento de transformação independente.Essas suspensões podem ser então subcultivadas e mantidas na forma deconjuntos de embriões imaturos ou regeneradas em plantas inteiras, por meiode maturação e germinação de embriões somáticos individuais.

Exemplo 11

Expressão de Construções de DNA Recombinante em Células Microbianas

Os cDNAs codificadores dos polipeptídeos do presente podemser inseridos no vetor de expressão pBT430 de E. coli T7. Este vetor é umderivado de pET-3a (Rosenberg et al (1987), Gene 56: 125-135), queemprega o sistema bacteriófago T7 RNA polimerase/T7 promotor. Oplasmídeo pBT430 foi construído por meio, primeiramente, de destruiçãodos locais EcoR I e Hind Ill em pET-3a nas suas posições originais.

Adaptador de oligonucleotídeos que contém locais EcoR I e Hind Ill foiinserido no local BamH I de pET-3a. Isso criou pET-3aM com locais declonagem exclusivos adicionais para inserção de genes no vetor deexpressão. Em seguida, o local Nde I na posição de início de tradução foiconvertido em local Nco I, utilizando mutagênese dirigida poroligonucleotídeos. A seqüência de DNA de pET-3aM nessa região, 5'-CATATGG, foi convertida em 5'-CCCATGG em pBT430.

DNA de plasmídeo que contém cDNA pode, por exemplo, seradequadamente digerido para liberar fragmento de ácido nucléico codificador daproteína. Este fragmento pode ser purificado em seguida sobre gel de agarosede baixa fusão NuSieve GTG® a 1% (FMC). Tampão e agarose contêm 10pg/ml de brometo de etídio para visualização do fragmento de DNA. O fragmentopode ser purificado em seguida a partir do gel de agarose por meio de digestãocom GELase® (Epicentre Technologies), de acordo com as instruções dofabricante, precipitado em etanol, seco e novamente suspenso em 20 μΙ deágua. Adaptadores de oligonucleotídeos apropriados podem ser ligados aofragmento u tilizando T4 DNA Iigase (New England Biolabs, Beverly MA). Ofragmento que contém os adaptadores ligados pode ser purificado a partir doexcesso de adaptadores, utilizando agarose de baixa fusão conforme descritoacima. O vetor pBT430 é digerido, desfosforilado com fosfatase alcalina (NEB) edesproteinado com fenol/clorofórmio, conforme descrito acima. O vetorpreparado pBT430 e o fragmento podem ser ligados em seguida a 16°C porquinze horas, seguido por transformação em células eletrocompetentes DH5(GIBCO BRL). Os transformadores podem ser selecionados sobre placas agarque contêm meios LB e 100 pg/ml de ampicilina. Os transformadores quecontêm o gene codificador dos polipeptídeos do presente são selecionados emseguida para orientação correta com relação ao promotor T7 por meio de análisede enzimas de restrição.

Para expressão de alto nível, clone de plasmídeo com o insertode cDNA na orientação correta com relação ao promotor T7 pode sertransformado em linhagem BL21 (DE3) de E. coli (Studier et al (1986), J. Mol.Biol. 189: 113-130). Os cultivos crescem em meio LB contendo ampicilina (100mg/l) a 25°C. Sob densidade ótica a 600 nm de cerca de 1, pode-se adicionarIPTG (isopropiltio-p-galactosida, o indutor) até concentração final de 0,4 mM epode-se prosseguir com a incubação por três horas a 25°C. As células sãocolhidas em seguida por meio de centrifugação e novamente suspensas em 50μl de 50 mM Tris-HCI a pH 8,0 contendo 0,1 mM de DT T e 0,2 mM demetilsulfonil fluoreto de fenila. Pequena quantidade de esferas de vidro de 1mm pode ser adicionada e a mistura sonicada por três vezes, por cerca decinco segundos cada vez, com sonicador de microssonda. A mistura écentrifugada e a concentração de proteínas do sobrenadante é determinada.Um μg de proteína da fração solúvel do cultivo pode ser separado por meio deeletroforese de gel de poliacrilamida-SDS. Pode-se observar nos géis as faixasde proteína migrando no peso molecular esperado.

Exemplo 12

Eletroporação de Agrobacterium tumefaciens LBA4404:Células competentes de eletroporação (40 μΙ), tais comoAgrobacterium tumefaciens LBA4404 (contendo PHP10523), sãodescongeladas sobre gelo (vinte a trinta minutos). PHP10523 contém genesVIR para transferência de T-DNA1 origem de reprodução de plasmídeos combaixo número de cópias de Agrobacterium, gene de resistência a tetraciclina elocal Cos para recombinação bimolecular de DNA in vivo. PHP10523 éadicionalmente descrito no Exemplo 17. Enquanto isso, a cubeta deeletroporação é resfriada sobre gelo. As configurações do eletroporador sãoajustadas a 2,1 kV. Parcela de DNA (0,5 μΙ de DNA parental em concentraçãode 0,2 pg a 1,0 μg em tampão com baixo teor de sal ou H2O destilada duasvezes) é misturada com as células de Agrobacterium tumefaciens LBA4404descongeladas ainda sobre gelo. A mistura é transferida para o fundo dacubeta de eletroporação e mantida em repouso sobre gelo por um a doisminutos. As células são eletroporadas (eletroporador Eppendorf 2510)pressionando-se a tecla "pulso" duas vezes (atingindo idealmente pulso de 4,0milissegundos). Em seguida, 0,5 ml de meio 2xYT à temperatura ambiente (oumeio SOC) são adicionados à cubeta e transferidos para tubo com tampa deencaixe de 15 ml (tal como tubo Falcon®). As células são incubadas a 28-300C, 200-250 rpm por três horas.

Parcelas de 250 μΙ são espalhadas sobre placas contendo meioYM e 50 pg/ml de espectinomicina e incubadas por três dias a 28-30 0C. Paraaumentar a quantidade de transformadores, pode-se realizar uma dentre duasetapas opcionais:

Opção 1: sobrepor as placas com 30 μΙ de 15 mg/ml de rifampicin.LBA4404 possui gene de resistência cromossômica para rifampicin. Estaseleção adicional elimina algumas colônias contaminadoras observadas aoutilizar-se preparações mais fracas de células competentes LBA4404.

Opção 2: realizar duas réplicas da eletroporação para compensaras células eletrocompetentes mais fracas.

Identificação de transformadoresQuatro colônias independentes são tomadas e riscadas sobreplacas que contêm meio mínimo AB e 50 pg/ml de espectinomicin paraisolamento de colônias isoladas. As placas são incubadas a 28 0C por doisa três dias. Uma única colônia para cada suposto cointegrado é tomada einoculada com 4 ml de 10 g/l de bactopeptona, 10 g/l de extrato delevedura, 5 g/l de cloreto de sódio e 50 mg/l de espectinomicin. A mistura éincubada por 24 horas a 28 0C mediante agitação. DNA de plasmídeo de 4ml de cultivo é isolado utilizando Qiagen Miniprep e lavagem de TampãoBP opcional. O DNA é eluído em 30 μΙ. Parcelas de 2 μΙ são utilizadas paraeletroporar 20 μΙ de DH10b + 20 μΙ de H2O destilada duas vezes conformeacima. Opcionalmente, parcela de 15 μΙ pode ser utilizada para transformar75 a 100 μΙ de DH5a com Eficiência de Biblioteca Invitrogen. As células são espalhadas sobre placas que contêm meio LB e 50 pg/ml deespectinomicin e incubadas a 37 0C por uma noite.

Três a quatro colônias independentes são tomadas para cadasuposto cointegrado e inoculadas com 4 ml de meio 2xYT (10 g/l debactopeptona, 10 g/l de extrato de levedura, 5 g/l de cloreto de sódio), com 50 pg/ml de espectinomicin. As células são incubadas a 37 0C por uma noite comagitação. Em seguida, isolar o DNA de plasmídeo de 4 ml de cultivo utilizandoMiniprep QIAprep® com lavagem de Tampão PB opcional (eluir em 50 μΙ).Utilizar 8 μΙ para digestão com Sall (utilizando DNA parental e PHP10523 comocontroles). Três outras digestões utilizando enzimas de restrição BamHI1 EcoRIe Hindlll são realizadas para quatro plasmídeos que representam doissupostos cointegrados com padrão de digestão de Sall correto (utilizando DNAparental e PHP10523 como controles). Géis eletrônicos são recomendadospara comparação.Exemplo 13

Transformação de Milho Utilizando Agrobacterium

Transformação de milho mediada por Agrobacterium é realizadaessencialmente conforme descrito por Zhao et al em Meth. Mol. Biol. 318: 315-323 (2006) (vide também Zhao et al, Moi Breed. 8: 323-333 (2001) e PatenteNorte-Americana n° 5.981.840 emitida em nove de novembro de 1999,incorporada ao presente como referência). O processo de transformação envolveinoculação de bactérias, cocultivo, repouso, seleção e regeneração de plantas.

1. Preparação de embriões imaturos:

Embriões de milho imaturos são dissecados de cariopses ecolocados em microtubo de 2 ml que contém 2 ml de meio PHI-A.

2. Infecção com Agrobacterium e cocultivo de embriõesimaturos:

2.1 Etapa de infecção

Meio PHI-A de (1) é removido com 1 ml de micropipetador eadiciona-se 1 ml de suspensão de Agrobacterium (incluindo, mas sem limitar-se ao Agrobacterium descrito no Exemplo 7). O tubo é suavemente invertidopara mistura. A mistura é incubada por cinco minutos à temperatura ambiente.

2.2 Etapa de cocultivo

A suspensão de Agrobacterium é removida da etapa de infecçãocom micropipetador de 1 ml. Utilizando espátula estéril, os embriões sãoraspados do tubo e transferidos para placa de meio PHI-B em placa Petri de100 χ 15 mm. Os embriões são orientados com o eixo embriônico para baixosobre a superfície do meio. Placas com os embriões são cultivadas a 20 0C1 noescuro, por três dias. L-cisteína pode ser utilizada na fase de cocultivo. Com ovetor binário padrão, o meio de cocultivo que recebeu 100 a 400 mg/l de L-cisteína é crítico para a recuperação de eventos transgênicos estáveis.

3. Seleção de supostos eventos transgênicos:A cada placa de meio PHI-D em placa Petri 100 χ 15 mm, dezembriões são transferidos, mantendo a orientação, e as placas são vedadascom parafina. As placas são incubadas no escuro a 28°C. Espera-se quesupostos eventos de crescimento ativo, na forma de tecido embriônico amareloclaro, sejam visíveis em seis a oito semanas. Embriões que não produzemeventos podem ser marrons e necróticos e pouco crescimento de tecidoesboroadiço é aparente. Suposto tecido embriônico transgênico é subcultivadoem placas PHI-D novas em intervalos de duas a três semanas, dependendo davelocidade de crescimento. Os eventos são registrados.

4. Regeneração de plantas TO:

Tecido embriônico propagado sobre meio PHI-D é subcultivadoem meio PHI-E (meio de amadurecimento de embriões somáticos), em cemplacas Petri de 25 mm e incubado a 28°C, no escuro, até o amadurecimentode embriões somáticos, por cerca de dez a dezoito dias. Embriões somáticosamadurecidos individuais com escutelo e coleóptilo bem definidos sãotransferidos para meio de germinação de embriões PHI-F e incubados a 28°Cna luz (cerca de 80 μΕ de lâmpadas fluorescentes brancas frias ouequivalentes). Em sete a dez dias, plantas regeneradas, com cerca de 10 cmde altura, são colocadas em vasos em mistura para hortas e estabilizadasutilizando métodos padrão de horticultura.

Meios para transformação de plantas

1. PHI-A: 4 g/l de sais básicos CHU, 1,0 ml/l de mistura de1000X vitamina de Eriksson, 0,5 mg/l de tiamin HCI, 1,5 mg/l de 2,4-D, 0,69 g/lde L-prolina, 68,5 g/l de sacarose, 36 g/l de glicose, pH 5,2. Adicionar 100 u.mde acetosiringona (esterilizada em filtro).

2. PHI-B: PHI-A sem glicose, aumentar 2,4-D para 2 mg/l,reduzir sacarose para 30 g/l e suplementar com 0,85 mg/l de nitrato de prata(esterilizado em filtro), 3,0 g/l de Gelrite®, 100 μΜ de acetosiringona(esterilizada em filtro), pH 5,8.

3. PHI-C: PHI-B sem Gelrite® e acetosiringona, reduzir 2,4-Dpara 1,5 mg/l e suplementar com 8,0 g/l de agar, 0,5 g/l de tampão ácido 2-[N-morfolino]etano-sulfônico (MES), 100 mg/l de carbenicilin (esterilizada em filtro).

4. PHl-D: PHI-C suplementado com 3 mg/l de bialafós(esterilizado em filtro).

5. PHI-E: 4,3 g/l de sais de Murashige e Skoog (MS) (Gibco,BRL 11117-074), 0,5 mg/l de ácido nicotínico, 0,1 mg/l de tiamina HCI, 0,5 mg/lde piridoxina HCI, 2,0 mg/l de glicina, 0,1 g/l de mio-inositol, 0,5 mg/l de zeatin(Sigma, Cat. N0 Z-0164), 1 mg/l de ácido indol acético (IAA), 26,4 Mg/l de ácidoabscísico (ABA), 60 g/l de sacarose, 3 mg/l de bialafós (esterilizado em filtro),100 mg/l de carbenicilin (esterilizado em filtro), 8 g/l de agar, pH 5,6.

6. PHI-F: PHI-E sem zeatin, IAA, ABA; reduzir sacarose para40 g/l; substituir agar com 1,5 g/l de Gelrite®; pH 5,6.

As plantas podem ser regeneradas a partir do calo transgênicopor meio de transferência, em primeiro lugar, de conjuntos de tecido para meioN6 suplementado com 0,2 mg por litro de 2,4-D. Após duas semanas, o tecidopode ser transferido para meio de regeneração (Fromm et al, Bio/Technology 8:833-839 (1990)). Plantas TO transgênicas podem ser regeneradas e o seufenótipo determinado. Semente T1 pode ser recolhida.

Além disso, construção de DNA recombinante que contém genede Arabidopsis validado pode ser introduzida em linhagem congênita de milhode elite por meio de transformação direta ou introgressão a partir de linhagemtransformada separadamente.

Plantas transgênicas, sejam elas cultivadas ou híbridas, podempassar por experimentos de campo mais vigorosos para estudo do aumento dorendimento e/ou estabilidade sob condições de limitação de nitrogênio e semlimitação de nitrogênio.Pode-se realizar análise de rendimento subseqüente paradeterminar se as plantas que contêm o gene líder de Arabidopsis validadoapresentam melhoria do desempenho de rendimento (sob condições delimitação ou sem limitação de nitrogênio), em comparação com as plantascontrole (ou referência) que não contêm o gene líder de Arabidopsis validado.Plantas que contêm o gene líder de Arabidopsis validado apresentariam menorperda de rendimento com relação às plantas controle, preferencialmente 50%menos perda de rendimento, sob condições de limitação de nitrogênio, ouapresentariam aumento de rendimento com relação às plantas controle sobcondições sem limitação de nitrogênio.

Exemplo 14

Avaliação de Compostos para Determinar a sua Capacidade de Inibir aAtividade de Transportadores de Nitrato

Os polipeptídeos descritos no presente podem ser produzidosutilizando qualquer quantidade de métodos conhecidos pelos técnicos noassunto. Estes métodos incluem, mas sem limitar-se a expressão em bactériasconforme descrito no Exemplo 11 ou expressão em cultivo de célulaseucarióticas, in planta e utilizando sistemas de expressão viral em linhagenscelulares ou organismos adequadamente infectados. Os polipeptídeos dopresente podem ser expressos como formas maduras das proteínas conformeobservado in vivo ou como proteínas de fusão por meio de ligação covalente auma série de enzimas, proteínas ou marcas de afinidade. Parceiros deproteínas de fusão comuns incluem glutationa S-transferase ("GST"), tioredoxin("Trx"), proteína de união de maltose e polipeptídeo hexahistidina C e/ou N-terminal ("(His)6")- As proteínas de fusão podem ser elaboradas com local dereconhecimento de protease no ponto de fusão, de forma que os parceiros defusão possam ser separados por meio de digestão de protease para gerarenzima madura intacta. Exemplos dessas proteases incluem trombina,enteroquinase e fator Xa. Pode-se utilizar, entretanto, qualquer protease quedivida especificamente o peptídeo que conecta a proteína de fusão e a enzima.

A purificação dos polipeptídeos do presente, se desejado, podeutilizar qualquer quantidade de tecnologias de separação familiares para ostécnicos no assunto de purificação de proteínas. Exemplos desses métodosincluem, mas sem limitar-se a homogeneização, filtragem, centrifugação,desnaturação a quente, precipitação de sulfato de amônio, dessalinização,precipitação de pH, cromatografia de troca de íons, cromatografia de interaçãohidrofóbica e cromatografia de afinidade, em que o Iigante de afinidaderepresenta substrato, análogo de substrato ou inibidor. Quando ospolipeptídeos do presente forem expressos na forma de proteínas de fusão, oprotocolo de purificação pode incluir o uso de resina de afinidade, que éespecífica para a marca de proteína de fusão ligada à enzima expressa ouresina de afinidade que contém Iigantes que sejam específicos para a enzima.

Os polipeptídeos do presente podem ser expressos, por exemplo, na forma deproteína de fusão acoplada ao terminal C de tioredoxin. Além disso, peptídeo(His)6 pode ser elaborado no terminal N da porção de tioredoxin fundida paragerar oportunidades adicionais de purificação de afinidade. Outras resinas deafinidade apropriadas poderão ser sintetizadas por meio de ligação dos Iigantesapropriados a qualquer resina adequada, tal como Sepharose 4B. Emrealização alternativa, proteína de fusão de tioredoxina pode ser eluídautilizando ditiotreitol; entretanto, a eluição pode ser obtida utilizando outrosreagentes que interagem para deslocar a tioredoxina da resina. Estesreagentes incluem β-mercaptoetanol ou outro tiol reduzido. A proteína de fusãoeluída pode ser submetida a purificação adicional por meios tradicionais,conforme indicado acima, se desejado. A divisão proteolítica da proteína defusão de tioredoxina e a enzima pode ser realizada após a purificação daproteína de fusão ou enquanto a proteína ainda estiver unida à resina deafinidade ThioBond® ou outra resina.

Enzima bruta, parcialmente purificada ou purificada, sejaisoladamente ou na forma de proteína de fusão, pode ser utilizada em testes deavaliação de compostos para determinar a sua capacidade de inibição daativação enzimática dos polipeptídeos descritos no presente. Podem serconduzidos testes sob condições experimentais bem conhecidas que permitama atividade enzimática ideal.

Testes que permitem a rápida seleção da atividade de transportede nitrato foram descritos na literatura, incluindo, mas sem limitar-se a testeque mede a absorção de nitrato enriquecido com 15N em oócitos de Xenopusque expressam as proteínas (Tong et al, The Plant J. (2005) 41: 442-450).

Exemplo 15

Expansão da Faixa de Absorção de Nitrato Linear de HATS de PlantasSuperiores por Meio de Alteração Genética

HATs conhecidamente possuem baixo Km (na faixa de 10 a100 μΜ) e baixo Vmax (Doddema et al, Kinetics Physiol. Plant (1979) 45:332-338, Meharg et al (1995), J. Membr. Bioi 145: 49-66, Touraine et al,Plant Physiol. (1997) 114: 137-144, Liu et al, Plant Cell (1999) 11 (5): 865-874). A velocidade de absorção de HATs permanece constante, portanto,desde que a concentração de nitrato atinja nível cerca de duas a três vezesmais alto que o seu Km.

A concentração de nitrato no campo mais relevante é de cerca de2 a 5 mM em fazenda de milho moderna típica. Dentro desta faixa deconcentração, a velocidade de absorção de HATs é bem saturada. A extensãoda absorção de nitrato linear de HATs de muito baixa para concentração nocampo relevante permitiria que a safra de milho utilizasse totalmente o nitratodisponível para melhor crescimento e produtividade. Esse transportadortambém permitiria que a planta de safra mantivesse a eficiência de absorçãonormal em ingresso de nitrato mais baixo pela sua maior capacidade deabsorção rápida em concentração de nitrato relativamente mais baixa.

Vários métodos de alteração genética (Stemmet, W. P., PNAS(1994) 91: 10747-10751, Crameri et al, Nature (1998) 391: 288-291, Ness et al, Nature Biotech. (1999) 17: 893-896) podem ser utilizados para gerar diferentestipos de bibliotecas de HATs alterados. Podem ser geradas bibliotecas, porexemplo, por alteração de gene familiar e gene único. Diversidades adicionaispodem ser introduzidas por oligos bloqueados que conduzem mutações deaminoácidos.

As bibliotecas de HAT alteradas podem ser expressas funcionalmenteem um dos hospedeiros heterólogos, tais como levedura, E. coli e algas verdes.Preferencialmente, o hospedeiro não possui o processo de assimilação de nitrato,exceto por nitrato reductase endógena ou introduzida. A velocidade de absorção denitrato por alteradores expressos funcionalmente pode ser testada por meio de medição direta ou esgotamento de nitrato no meio de teste por meio de HPLC ououtros meios analíticos ou por medição de nitrito gerado por nitrato reductase namesma célula. A concentração de nitrito pode ser facilmente determinada por meiode teste colorimétrico (tal como o uso de Reagente Greiss) ou outro meio analítico(HPLC). Caracterização adicional das supostas coincidências da seleção de váriasbibliotecas alteradas pode ser atingida por meio de medição das velocidades deabsorção contra diferentes concentrações de nitrato. Este teste forneceráparâmetros cinéticos de absorção de Km e Vmax.

Coincidências confirmadas com propriedades aprimoradas podemser novamente alteradas em seguida para gerar segunda rodada de bibliotecasalteradas e o esquema de seleção mencionado acima pode ser utilizado paraidentificar coincidências de segunda rodada. Este processo pode ser repetidoaté que sejam identificadas diversas variantes alteradas que atendam àspropriedades cinéticas desejadas.Exemplo 16

Isolamento. Clonagem ε Seqüenciamento do Promotor Nar da LinhagemCongênita B73 de Milho

Identificação de clone de BAC que conduz o gene Nar

Biblioteca de BAC derivada da linhagem congênita B71 de milhofoi selecionada por meio de PCR utilizando os primers frontal e reversoilustrados em SEQ ID N0 75 e 76, respectivamente. As condições de cicloforam etapa de ativação inicial a 95°C por quinze minutos, seguida por 35ciclos a 94°C por um minuto, 60°C por um minuto e 72°C por um minuto. Aextensão final foi a 72°C por dez minutos. Foi obtido produto de 377 bp. Oclone de BAC ZMMBBb0521a1 foi identificado como conduzindo o gene Nar.

Clonagem do promotor Nar da linhagem congênita B73 de milho

O promotor Nar foi clonado por meio de PCR utilizando o primerfrontal e reverso com locais de enzimas de restrição para BamHI e Hindlllilustrados em SEQ ID N0 77 e 78, respectivamente.

A 1 μl de DNA de BAC diluído (1:100) do clone de BACZMMBBb0521a1, foram adicionados 1 μl de mistura de primer emconcentração de 10 μΜ cada, 4 μl de DNTPs em concentração de 2,5 mM, 10μl de 5x tampão HF e 33,5 μl de H2O e 0,5 μl de DNA Polimerase de AltaFidelidade Phusion (Finnzymes). As condições de ciclo foram etapa deativação inicial a 98°C por trinta segundos, seguida por 35 ciclos de 98°C pordez segundos, 63°C por trinta segundos e 72°C por um minuto. A extensãofinal foi a 72°C por dez minutos. Foi obtido produto de 3621 bp. O produto de3621 bp foi purificado com gel utilizando o Kit de Extração de Gel Qiaquick®(Qiagen) e eluído com 88 μΙ de Tampão de Eluição.

À faixa purificada, foram adicionados 10 μl de tampão E(Promega) e 1 μl de cada uma das enzimas de restrição, BamHI e Hind III (a 10U/μl cada). A mistura de teste foi incubada a 37°C por três horas e limpa comKit de Purificação de PCR Qiaquick® (Qiagen).

0 vetor pENTR-5' (SEQ ID N0 85) foi digerido com BamHI eHindlll e desfosforilado. A faixa de PCR purificada foi inserida no vetor pENTR-5' preparado utilizando o Kit de Ligação Rápida Epicentre. A mistura de reaçãode ligação continha 1,5 μΙ de tampão (1 Ox), 1,5 μΙ de ATP (1 Ox), 1 μg de ligase,1 μΙ de vetor pENTR-5' (cerca de 10 ng/μΙ de BamHI/Hindlll/vetordesfosforilado), 1 μΙ de inserto de promotor (cerca de 30 ng) e 9 μΙ de H2O. Areação de ligação foi mantida em processamento por quinze minutos àtemperatura ambiente e foi suspensa por meio de incubação da mistura a 70 0Cpor quinze minutos.

Transformação em bactérias ε seleção por PCR em busca de inserto1 μΙ da mistura de ligação foi adicionado a 20 μΙ de célulaseletrocompetentes (DH10B EIectroMax-Invitrogen), a mistura foi eletroporada comPorador Celular Gibco BRL1 adicionou-se em seguida 1 ml de meios SOC e a misturafoi incubada em agitador a 37 0C por uma hora. 150 μΙ de células foram colocadossobre placas LB com seleção de canamicin e cultivados por uma noite a 37 0C.

Doze colônias foram tomadas e adicionou-se 30 μΙ de meios LB.As colônias foram selecionadas utilizando PCR. A 1 μΙ de DNA de colônia(colônia/30 μΙ de LB), foram adicionados 5 μΙ de 2x mistura mestre de HotTaq(Qiagen), 1 μΙ (10 mM de mistura de primers, SEQ ID N0 77 e 78) e 3 μΙ dedH20. As condições de ciclo foram ativação inicial a 95 0C por quinze minutos,seguida por 35 ciclos de 95 0C por cinqüenta segundos, 55 0C por cinqüentasegundos e 72 0C por quatro minutos.

A extensão final foi a 72 0C por dez minutos.

Seqüenciamento de insertos

DNA que conduz o inserto foi seqüenciado utilizando os primersde seqüências ilustrados em SEQ ID N0 79 a 84. A seqüência do inserto éexibida em SEQ ID N0 70. A construção de vetor que conduz o inserto de 3621bp foi denominada PHP27621 e é exibida em SEQ ID N0 86 e na Fig. 1.

Exemplo 17

Teste do Promotor de NAR em Milho Transgênico ε Arabidopsis

Utilizando tecnologia LR Clonase de portal da Invitrogen®,realizou-se reação de recombinação de LR MuItiSite Gateway® para criar ovetor binário promotor de NAR de milho::GUS::PINII, UBI::MO-PAT::PINII eLTP2::DS-RED PINII JT (PHP27660, SEQ ID N0 87 e Fig. 2). O vetorPHP27660 contém os conjuntos de expressão a seguir:

1. Conjunto de término promotor de ubiquitin::MO-PAT::PINIIque expressa o gene de resistência a herbicidas PAT utilizado para seleçãodurante o processo de transformação.

2. Conjunto de término promotor de LTP2::DS-RED2::Pinllque expressa o gene marcador de coloração DS-VERMELHO utilizado paraseleção de sementes.

3. Conjunto de término promotor de NAR::GUS::PINII queexpressa o gene GUS sob o controle do promotor de NAR de milho.

O vetor PHP27660 foi eletroporado utilizando o protocolo descritono Exemplo 16 em células de Agrobacterium LBA4404 que contêm PHP10523por meio de eletroporação, criando o vetor cointegrado final PHP27860 (SEQID N0 88 e Fig. 3), foi utilizado em seguida para transformação de milho combase em Agrobacterium conforme descrito no Exemplo 17. Plantastransgênicas TO foram amostradas para determinar a expressão de GUS.Separadamente, o mesmo vetor (PHP27860) também foi utilizado paratransformação de Arabidopsis, seguindo os procedimentos de mergulhamentode inflorescência padrão. Eventos transgênicos foram selecionados por meiode pulverização de herbicida glufosinato sobre as mudas T1. As plantas T1resistentes a herbicidas foram amostradas para determinar a expressão de GUS.

Amostras de tecido de folha e raiz foram recolhidas de plantastransgênicas em momentos diferentes, incluindo o estágio de muda e maduro.Amostras de tecidos recém colhidas foram dissecadas em pequenos pedaçospara facilitar a penetração da solução de manchas de GUS. Manchashistoquímicas de GUS foram realizadas seguindo o protocolo padrão(Jefferson1 R. A., Kavanagh1 Τ. A., Bevan1 M. W., 1987, Gus Fusions: Beta-Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in HigherPlants, EMBO J. 6 (13): 3901-3907) em incubação a 37 0C por uma noite.

Nenhuma atividade promotora significativa foi observada em milhotransgênico e tecidos de Arabidopsis.

Exemplo 18

Teste dos Efeitos de Seqüências de Junção Externas Sobre o Promotor deNAR em Milho Transgênico ε Arabidopsis

O sistema de clonagem Gateway deixa fragmento curto deseqüências de "pegadas" entre os componentes, particularmente fragmentoATT-B1 de 21 bp entre o promotor de NAR e a região de codificação de GUS.

Demonstrou-se que isso enfraquece ou até elimina a atividade promotora emcertos casos. Isso se refere provavelmente à distância física entre elementospromotores básicos e o códon de início. Para determinar se a introdução dolocal ATT-B1 está afetando negativamente o promotor de NAR1 construção quecontém o conjunto promotor NAR de milho::GUS::PINII é elaborada commétodo de clonagem convencional, ou seja, sem o uso do sistema Gateway.Plantas de milho transgênicas são produzidas por meio de transformação combase em Agrobacteriume várias amostras de tecidos são recolhidas paraestudo de expressão de GUS conforme descrito no Exemplo 17.

Exemplo 19

Teste do Promotor de NAR de Milho em Série de Exclusão

O gene NAR possui padrão de expressão específico de raiz eindutível por nitrato. Para determinar os fragmentos que determinam aespecificidade e a atividade promotora de NAR1 uma série de construções quecontêm fragmentos promotores de NAR truncados ligados às seqüências deGUS e a extremidade PINII é elaborada e testada conforme descrito para opromotor de comprimento total nos Exemplos 17 e 18.

Utilizando BLASTN (Ferramenta de Busca de Alinhamento Local

Básico; Altschul et al (1993), J. Moi Biol. 215: 403-410), podem seridentificadas seqüências no promotor de NAR que poderão ser importantespara aumentar ou suprimir a atividade promotora. A seqüência em volta de 1,5a 1,9 kb do promotor de NAR exibe homologia para outro gene e elemento detransposon. Espera-se, portanto, que a exclusão deste fragmento conforme exibidoem SEQ ID N0 89 adicione informações sobre a atividade promotora de NAR.

Além disso, a truncagem que reduz o comprimento do promotorconforme exibido em SEQ ID N0 71, 72, 73, 74 e 90 pode também ser testadada mesma forma descrita para o promotor de comprimento total nos Exemplos17 e 18. Subfragmentos promotores adicionais podem ser preparados utilizandoprimers derivados da seqüência promotora de NAR de 3,6 kb em PCR.

Exemplo 20

Avaliação da Absorção de Nitrato em Milho Utilizando Seqüências de HATε NAR ε Suas Combinações

Foram preparadas as construções de expressão de milho a seguirpara avaliação da absorção de nitrato em milho: PHP27280 (SEQ ID N0 93 eFig. 4), PHP27281 (SEQ ID N0 94 e Fig. 5), PHP27282 (SEQ ID N0 95 e Fig. 6)e PHP27283 (SEQ ID N0 96 e Fig. 7).

Construções adicionais que compreendem seqüências de HAT ecombinações de seqüências de HAT e Nar serão preparadas e testadas paradeterminar a sua capacidade de alteração de transporte de nitrato. TO, T1 egerações subseqüentes serão avaliadas para determinar a biomassa alterada eo peso total da espiga sob condições de 1 mM de nitrato.Listagem de Seqüências

<110> Ε. I. DU PONT DE NEMOURS AND COMPANY

<120> COMPONENTES DE TRANSPORTADORES DE NITRATO

<130> BB1555

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<170> PATENT IN VERSION 3.3

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<213> PRIMER

<4 00> 1

CCAACTGGAG TCCAACACCC ACAAA

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<211> 21

<212> DNA

<213> PRIMER

<400> 2

CATGCTGCTC GTCCACTGCG G

<210> 3

<211> 20

<212> DNA

<213> PRIMER

<4 00> 3

TAATACGACT CACTATAGGG

<210> 4

<211> 19

<212> DNA

<213> PRIMER

<4 00> 4

TATTTAGGTG ACACTATAG

<210> 5

<211> 20

<212> DNA

<213> PRIMER

<400> 5

ATGTTGTTGG TGGTGAGCTG<210> 6

<211> 18

<212> DNA

<213> PRIMER

<400> 6

ACACGAGGTT GGCCATGC

<210> 7

<211> 25

<212> DNA

<213> PRIMER

<400> 7

GTTTGACACC CCTTTTCTAG CAAGG

<210> 8

<211> 25

<212> DNA

<213> PRIMER

<400> 8

CCTTGCTAGA AAAGGGGTGT CAAAC

<210> 9

<211> 27

<212> DNA

<213> PRIMER

<400> 9

GGTCCCGTTT GGTTAGAGAG ACTAATC

<210> 10

<211> 22

<212> DNA

<213> PRIMER

<4 00> 10

GCGCAACGAA ATGCATTGGT CA

<210> 11

<211> 25

<212> DNA

<213> PRIMER

<400> 11

AGGGGAGAGA AGAGAAAAAG CGGGT<210> 12<211> 28<212> DNA<213> PRIMER

<4 00> 12

GCTGCATGTT TACGACTACA ATCTTTGG

<210> 13

<211> 25

<212> DNA

<213> PRIMER<400> 13

TTTGTGGGTG TTGGACTCCA GTTGG

<210> 14

<211> 23

<212> DNA

<213> PRIMER

<400> 14

TTTGTGGGTG TTGGACTCCA GTT

<210> 15

<211> 20

<212> DNA

<213> PRIMER

<400> 15

TTTGTGGGTG TTGGACTCCA

<210> 16

<211> 17

<212> DNA

<213> PRIMER

<4 00> 16

GGGATGACGC CGAAGGT

<210> 17

<211> 17

<212> DNA

<213> PRIMER

<400> 17

CTTCGGCGTC ATCCCCT

<210> 18

<211> 17

<212> DNA

<213> PRIMER

<400> 18

AAGGGGATGA CGCCGAA

<210> 19

<211> 17

<212> DNA

<213> PRIMER

<4 00> 19

TTCGGCGTCA TCCCCTT

<210> 20

<211> 21

<212> DNA

<213> PRIMER

<400> 20

CACATCGCCG TGGGCATCCT T<210> 21

<211> 18

<212> DNA

<213> PRIMER

<400> 21

AGGATGCCCA CGGCGATG

<210> 22

<211> 21

<212> DNA

<213> PRIMER

<400> 22

CACATCGCCG TGGGCATCCT

<210> 23

<211> 21

<212> DNA

<213> PRIMER

<400> 23

AAGGATGCCC ACGGCGATGT

<210> 24

<211> 18

<212> DNA

<213> PRIMER

<4 00> 24

TGCCCCGCGG TTAGCACA

<210> 25

<211> 18

<212> DNA

<213> PRIMER

<4 00> 25

TGTGCTAACC GCGGGGCA

<210> 26

<211> 18

<212> DNA

<213> PRIMER

<400> 26

GCGGTTAGCA CAAGGATG

<210> 27

<211> 18

<212> DNA

<213> PRIMER

<4 00> 27

CATCCTTGTG CTAACCGC

<210> 28

<211> 25

<212> DNA

<213> PRIMER<400> 28

GGTAGTTGGC GACGGCGTGC CAGAG

<210> 29

<211> 22

<212> DNA

<213> PRIMER

<400> 29

GCGACGGCGT GCCAGAGCAC CC

<210> 30

<211> 25

<212> DNA

<213> PRIMER

<4 00> 30

CAGGTTCTCC CGGATGATGG GGATC 25

<210> 31

<211> 26

<212> DNA

<213> PRIMER

<4 00> 31

GATCCCCATC ATCCGGGAGA ACCTGG 26

<210> 32

<211> 25

<212> DNA

<213> PRIMER

<4 00> 32

GATCCCCATC ATCCGGGAGA ACCTG 25

<210> 33

<211> 26

<212> DNA

<213> PRIMER

<4 00> 33

CCAGGTTCTC CCGGATGATG GGGATC 2 6

<210> 34<211> 3924<212> DNA

<213> Zea mays<400> 34

TTCGAGGGCA ATGGGTTCCA AAGAATGTCAAGGTTTTTTC TCCCCGAGTT AATTTGCTTCAGTTATTTGT TTTGACGGTT TATATATCCGTGACTTGACG GCACATGCAT GCATGCGTGGGCCAAACGTG CAATTGACTC ATTGAGTAGTACAAGCATTA ATATTTGCTG CATATATATATGCAACTTGA TCTTGTTAAT ΤΑΤΤΑΤΑΤΑΤCGACCATGCC TTGAGTAGAG CGTGAAAAATAGATGACACT ATTTGATATT GTTTAAAGATTTTAATCAGG GATGGTAGGG ACTAGTATTTATATACAATA ATAATAAGAA GCCACCAACCATGAGGGGTG TATCGGAATT TGACTTCCGA

TTTGAATTAG ACACTTAGTT ATTTATGAAA 60

CAAACTATAA TTAACCCTAA GCAAGGTGTT 120

TGTTAGCTTG GTGGCTAGCT TGTATCCATT 180

AGTGCACCGT GCGGCGGTTT GTGACGCGGT 24 0

CATCAGCAGG CTTGCGATCA TTAGACACTA 300

TATACACACA CATGCTTCAC TGACGACGCT 360

CCTAAGCACA ACGAACAAAC CTTAGATATG 4 20

AGGGGGTGAA AAAAAGGGAC GAGTAATTAT 4 80

GAGATAGGGA ATGTGCTGAA TAGATCAATT 54 0

CCTCTATGAT TTTCCATGTA ACACCTTTGA 600

TTTGAATTAT TATCTGTTCC AATATATTAG 660

GTTGTTCTTG CGTGTCCGTA CGCTCGTACG 720GTAGCTCGTT GGGTTGTTGT ACCAGCCATC CTGCTACTGC GCAACGAAAT GCATTGGTCA 780TCTCAATTAA GTCCAAAGAT TGTAGTCGTA AACATGCAGC CAATAAGAGC AAGGATAATA 840GTTTAGCCAT TGATATGTCT TCTAAAGCTA ATTATTACTG TATTGGACCC ACCTCGTACT 900CTCATTCTCT CACCACTTGT TTCGGAATCT GTACTGCTAC AACCAGCTCT TAGTCGACTG 960ATAATTAACT ACCCGCTTTT TCTCTTCTCT CCCCTCCAAC TGCAAAAATC TAATGTGGCA 1020AACCATTTAG CCTGCTTACA TCGTCAAAAA TCTAATGTGG TAAAGTGTGA AGTGTCCTAA 1080AGTTTTAGTC CTTAATTTCT TTCAATAAAC TAAACTAAAC TTTAGAAAAC TCAAACAAGT 1140CCTCATGTTT GCACATTTTA GGTCTCGTTT GGTTTGAGGG ACTAAAGATT AGTCCCTCCA 1200TTTTAGTCCC ATTTAGTTAC TAAATTACCA AACAGTAGGA CTAAAACAGG GACTAAATTG 1260TTTTAGTCCC TAGTCCCTTA AGATGGCTAA AAGGGACTAA ACCATATTAA TTCCACATTT 1320GCCCCTCATT TAGTTCAATT GTACTAATAG CAGGAGAATG TTAAAAGTCA TTTTAATCTT 1380CTTATGAGTC ATTTAGGCCC TGTTTGGTTC CATTAGTCAT AGAACTAAAG TTTAGTTGTA 1440GGGACTAAAT AGATTCTAAA TACATTAAAT GCAACACATA AAGACCAAAA TGCCCTTTTT 1500TGTTTGACAC CCCTTTTCTA GCAAGGGTAT TTGGAGTAAA TGTTGCCCTT TGGTCCCTTT 1560TAGCACCCAT GTGAGGGACT AGAGACTAAA ACCAATTAGT CCCTACTTTA GTCATTCCGT 1620TTAGCAAAAT AGAGACTAAA CGAGACTAAA AACGAGAGGC TAAAGATTAG TCTCTCTAAC 1680CAAACGGGAC CTAAAATTAC TATCTGTATG TATCTGTTGG ATGGAAAAGT CAGAACGTCG 17 4 0TGGGGACCAC CACGCTACCA CATGGTACGG TAATGTCAGA AAGTCGCTAT CTTCTTCGAT 1800CTGCATCTCC ACTCCAGCCA GCGCTGCTTA TCATCAGCAT TCACGAAGCC GCCCAACGAT 18 60AATAAAAAAT GTCAGCGCGA TCGCGCACTG CCTATAAAAC CCCGGCCGTC GCGTCCATGG 1920CGTTTCAGGA TCCGAGCACC AGAAAGAAGC TGAGTTAGCT AGGGTCAAGA AAGTAGTCAG 1980CACTCAGCAG GAAAAGAAGC AGAGACTACA CATCATGGCG AGTGACGCCG CGCATGGTAG 204 0CTCGCTGGAC GGGGTGACGC CGTCGAGCAA GTTCGACCTG CCGGTGGACT CGGAGCACAA 2100GGCCAAGACC ATCCGCCTGC TCTCCTTCGC GAACCCGCAC ATGCGTACCT TCCACCTCTC 2160CTGGATGTCC TTCTTCACCT GCGTCGTCTC CACCTTCGCG GCGGCGCCGC TGATCCCCAT 2220CATCCGGGAG AACCTGGGCC TGACCAAGGC CGACATCGGC AACGCCGGGG TGGCCTCCGT 2280CTCGGGCGCC ATCTTCTCGC GCCTCGCCAT GGGCGCCGTC TGCGACCTGC TGGGCCCGCG 234 0CTACGGCTGC GCCTTCGTCG TCATGCTGGC GGCGCCCGCG GTGTTCTGCA TGGCCGTCAT 24 00CGACAGCGCC GCGGGCTACG TCGCGTGCCG CTTCCTCATC GGCTTCTCCC TCGCCACCTT 24 60CGTCTCCTGC CAGTACTGGA CCAGCACCAT GTTCAACATC AAGATCATCG GCACCGTCAA 2520CGCGCTGGCG TCGGGGTGGG GCGACATGGG CGGCGGCGCC ACGCAGCTCA TCATGCCCTT 2580CGTCTACGAG GCCATCCTCC GCTGCGGCGC CACGCCGTTC GCCGCGTGGC GCATCGCCTA 2 64 0CTTCGTGCCG GGGATCATGC ACATCGCCGT GGGCATCCTT GTGCTAACCG CGGGGCAGGA 27 00CCTCCCCGAC GGCAACCTCC GCAGCCTCCG GAAGCAGCAG CAGCAGCAGC AGCAGGGTGA 27 60CGGCGGCGAT GCCAGCTGCT GCCGCAGGGA CAGCTTCTCC AGGGTGCTCT GGCACGCCGT 2820CGCCAACTAC CGCACCTGGG TCTTCGTCTT CGTGTACGGC TACAGCATGG GCGTGCAGCT 2880CACCACCAAC AACATCATCG CCGAGTTCTA CTACGACCAG TTCGAGCTCG ACATCCGCGT 2940GGCCGGCATC ATCGCCGCCT GCTTCGGCAT GGCCAACCTC GTGTCGCGGC CCCTGGGCGG 3000CGTGCTCTCC GACCTCGGCG CGCGGTACTG GGGCATGCGC GCGCGCCTCT GGAACATCTG 30 60GATCCTCCAG ACCGCCGGCG GCGCGTTCTG CTTCTGGCTC GGCCGCGCCA GCGAGCTCCC 3120GGCCTCCGTC ACCGCCATGG TGCTCTTCTC CTTCTGCGCG CAGGCCGCCT GCGGCGCCAC 3180CTTCGGCGTC ATCCCCTTCG TCTCCCGCCG CTCGCTGGGC GTCATCTCCG GGCTCACGGG 324 0CGCCGGCGGC AACGTGGGCG CCGGGCTCAC GCAGCTGCTC TTCTTCACCA CGTCCAGCTA 3300CTCCACGAGG AAGGGCATCG AGAACATGGG CATCATGGCC ATGGCGTGCA CGCTGCCGCT 3360CGTCCTCGTG CACTTCCCGC AGTGGGGTTC CATGCTCCTG CCGCCCAGCG CCGACGCCGA 3420CGAGGAGCGG TACTATGCCT CCGAGTGGAG CGAGGACGAG AAGAGCGTAG GCCGTCACAG 3480CGCAAGCCTA AAGTTCGCCG AGAACAGCCG GTCCGAGCGT GGCAAGCGCA ACGCCGTCGC 3540CGTCCTCGCC ACGGCCGCGG CCACGCCGGA GCACGTCGTG TAACAACTAG CGTACGTACT 3600TGTAGGTTCT GATCGAGCAT ACAGCAAACT GTGTAATGTA CTCTAGCAGT CTAGCTTGCT 3660CCGATACTCC TGCTTCCAAC AAAATTATGA AACATAGGCT AATATGGATC GGTGTACACG 3720TACGTCGTAG TATTTCCTGT GCAACATACA CAATTCAGTA AATGAACAAA CTTTGCTCAT 37 80GTGCATTCTT CTGCAAAGTA CAAATAAAAT CAAATAGAGA GGCCAGGACA ACGTCTATGA 38 40TCTATCAACT TGGTTGTTAA AATTAAAGAA AACCAACTGG AGTCCAACAC CCACAAAACA 3900TTTTGTCTCT AACACGTTGT TGTC 3924

<210> 35

<211> 1569

<212> DNA

<213> Zea mays<400> 35

ATGGCGAGTG ACGCCGCGCA TGGTAGCTCGGACCTGCCGG TGGACTCGGA GCACAAGGCCCCGCACATGC GTACCTTCCA CCTCTCCTGGTTCGCGGCGG CGCCGCTGAT CCCCATCATCATCGGCAACG CCGGGGTGGC CTCCGTCTCGGCCGTCTGCG ACCTGCTGGG CCCGCGCTACCCCGCGGTGT TCTGCATGGC CGTCATCGACCTCATCGGCT TCTCCCTCGC CACCTTCGTCAACATCAAGA TCATCGGCAC CGTCAACGCGGGCGCCACGC AGCTCATCAT GCCCTTCGTCCCGTTCGCCG CGTGGCGCAT CGCCTACTTCATCCTTGTGC TAACCGCGGG GCAGGACCTCCAGCAGCAGC AGCAGCAGCA GGGTGACGGCTTCTCCAGGG TGCTCTGGCA CGCCGTCGCCTACGGCTACA GCATGGGCGT GCAGCTCACCGACCAGTTCG AGCTCGACAT CCGCGTGGCCAACCTCGTGT CGCGGCCCCT GGGCGGCGTGATGCGCGCGC GCCTCTGGAA CATCTGGATCTGGCTCGGCC GCGCCAGCGA GCTCCCGGCCTGCGCGCAGG CCGCCTGCGG CGCCACCTTCCTGGGCGTCA TCTCCGGGCT CACGGGCGCCCTGCTCTTCT TCACCACGTC CAGCTACTCCATGGCCATGG CGTGCACGCT GCCGCTCGTCCTCCTGCCGC CCAGCGCCGA CGCCGACGAGGACGAGAAGA GCGTAGGCCG TCACAGCGCAGAGCGTGGCA AGCGCAACGC CGTCGCCGTCGTCGTGTAA

<210> 36

<211> 522

<212> PRT

<213> Zea mays

<400> 36

CTGGACGGGG TGACGCCGTC GAGCAAGTTC 60AAGACCATCC GCCTGCTCTC CTTCGCGAAC 120ATGTCCTTCT TCACCTGCGT CGTCTCCACC 180CGGGAGAACC TGGGCCTGAC CAAGGCCGAC 24 0GGCGCCATCT TCTCGCGCCT CGCCATGGGC 300GGCTGCGCCT TCGTCGTCAT GCTGGCGGCG 360AGCGCCGCGG GCTACGTCGC GTGCCGCTTC 420TCCTGCCAGT ACTGGACCAG CACCATGTTC 4 80CTGGCGTCGG GGTGGGGCGA CATGGGCGGC 54 0TACGAGGCCA TCCTCCGCTG CGGCGCCACG 600GTGCCGGGGA TCATGCACAT CGCCGTGGGC 660CCCGACGGCA ACCTCCGCAG CCTCCGGAAG 720GGCGATGCCA GCTGCTGCCG CAGGGACAGC 780AACTACCGCA CCTGGGTCTT CGTCTTCGTG 84 0ACCAACAACA TCATCGCCGA GTTCTACTAC 900GGCATCATCG CCGCCTGCTT CGGCATGGCC 960CTCTCCGACC TCGGCGCGCG GTACTGGGGC 1020CTCCAGACCG CCGGCGGCGC GTTCTGCTTC 1080TCCGTCACCG CCATGGTGCT CTTCTCCTTC 114 0GGCGTCATCC CCTTCGTCTC CCGCCGCTCG 1200GGCGGCAACG TGGGCGCCGG GCTCACGCAG 1260ACGAGGAAGG GCATCGAGAA CATGGGCATC 1320CTCGTGCACT TCCCGCAGTG GGGTTCCATG 1380GAGCGGTACT ATGCCTCCGA GTGGAGCGAG 14 40AGCCTAAAGT TCGCCGAGAA CAGCCGGTCC 1500CTCGCCACGG CCGCGGCCAC GCCGGAGCAC 1560

1569

MET ALA SER ASP ALA ALA HIS GLY SER SER LEU ASP GLY VAL THR PRO1 5 10 15

SER SER LYS PHE ASP LEU PRO VAL ASP SER GLU HIS LYS ALA LYS THR20 25 30

ILE ARG LEU LEU SER PHE ALA ASN PRO HIS MET ARG THR PHE HIS LEU35 40 45

SER TRP MET SER PHE PHE THR CYS VAL VAL SER THR PHE ALA ALA ALA50 55 60

PRO LEU ILE PRO ILE ILE ARG GLU ASN LEU GLY LEU THR LYS ALA ASP65 70 75 80

ILE GLY ASN ALA GLY VAL AI4A SER VAL SER GLY ALA ILE PHE SER ARG85 90 95

LEU ALA MET GLY ALA VAL CYS ASP LEU LEU GLY PRO ARG TYR GLY CYS100 105 HO

ALA PHE VAL VAL MET LEU ALA ALA PRO ALA VAL PHE CYS MET ALA VAL

115 120 125ILE ASP SER ALA ALA GLY TYR VAL ALA CYS ARG PHE LEU ILE GLY PHE130 135 140

SER LEU ALA THR PHE VAL SER CYS GLN TYR TRP THR SER THR MET PHE145 150 155 160

ASN ILE LYS ILE ILE GLY THR VAL ASN ALA LEU ALA SER GLY TRP GLY165 170 175

ASP MET GLY GLY GLY ALA THR GLN LEU ILE MET PRO PHE VAL TYR GLU180 185 190

ALA ILE LEU ARG CYS GLY ALA THR PRO PHE ALA ALA TRP ARG ILE ALA195 200 205

TYR PHE VAL PRO GLY ILE MET HIS ILE ALA VAL GLY ILE LEU VAL LEU210 215 220

THR ALA GLY GLN ASP LEU PRO ASP GLY ASN LEU ARG SER LEU ARG LYS225 230 235 240

GLN GLN GLN GLN GLN GLN GLN GLY ASP GLY GLY ASP ALA SER CYS CYS245 250 255

ARG ARG ASP SER PHE SER ARG VAL LEU TRP HIS ALA VAL ALA ASN TYR260 265 270

ARG THR TRP VAL PHE VAL PHE VAL TYR GLY TYR SER MET GLY VAL GLN275 280 285

LEU THR THR ASN ASN ILE ILE ALA GLU PHE TYR TYR ASP GLN PHE GLU290 295 300

LEU ASP ILE ARG VAL ALA GLY ILE ILE ALA ALA CYS PHE GLY MET ALA305 310 315 320

ASN LEU VAL SER ARG PRO LEU GLY GLY VAL LEU SER ASP LEU GLY ALA325 330 335

ARG TYR TRP GLY MET ARG ALA ARG LEU TRP ASN ILE TRP ILE LEU GLN340 345 350

THR ALA GLY GLY ALA PHE CYS PHE TRP LEU GLY ARG ALA SER GLU LEU355 360 365

PRO ALA SER VAL THR ALA MET VAL LEU PHE SER PHE CYS ALA GLN ALA370 375 380

ALA CYS GLY ALA THR PHE GLY VAL ILE PRO PHE VAL SER ARG ARG SER385 390 395 400

LEU GLY VAL ILE SER GLY LEU THR GLY ALA GLY GLY ASN VAL GLY ALA405 410 415

GLY LEU THR GLN LEU LEU PHE PHE THR THR SER SER TYR SER THR ARG420 425 430

LYS GLY ILE GLU ASN MET GLY ILE MET ALA MET ALA CYS THR LEU PRO435 440 445LEU VAL LEU VAL HIS PHE PRO GLN TRP GLY SER MET LEU LEU PRO PRO450 455 460

SER ALA ASP ALA ASP GLU GLU ARG TYR TYR ALA SER GLU TRP SER GLU465 470 475 480

ASP GLU LYS SER VAL GLY ARG HIS SER ALA SER LEU LYS PHE ALA GLU485 490 495

ASN SER ARG SER GLU ARG GLY LYS ARG ASN ALA VAL ALA VAL LEU ALA500 505 510

THR ALA ALA ALA THR PRO GLU HIS VAL VAL515 520

<210> 37

<211> 2014

<212> DNA

<213> Zea mays

<4 00> 37

TTCGAGGGCA ATGGGTTCCA AAGAATGTCA TTTGAATTAG ACACTTAGTT ATTTATGAAA 60AGGTTTTTTC TCCCCGAGTT AATTTGCTTC CAAACTATAA TTAACCCTAA GCAAGGTGTT 120AGTTATTTGT TTTGACGGTT TATATATCCG TGTTAGCTTG GTGGCTAGCT TGTATCCATT 180TGACTTGACG GCACATGCAT GCATGCGTGG AGTGCACCGT GCGGCGGTTT GTGACGCGGT 240GCCAAACGTG CAATTGACTC ATTGAGTAGT CATCAGCAGG CTTGCGATCA TTAGACACTA 300ACAAGCATTA ATATTTGCTG CATATATATA TATACACACA CATGCTTCAC TGACGACGCT 360TGCAACTTGA TCTTGTTAAT TATTATATAT CCTAAGCACA ACGAACAAAC CTTAGATATG 4 20CGACCATGCC TTGAGTAGAG CGTGAAAAAT AGGGGGTGAA AAAAAGGGAC GAGTAATTAT 4 80AGATGACACT ATTTGATATT GTTTAAAGAT GAGATAGGGA ATGTGCTGAA TAGATCAATT 54 0TTTAATCAGG GATGGTAGGG ACTAGTATTT CCTCTATGAT TTTCCATGTA ACACCTTTGA 600ATATACAATA ATAATAAGAA GCCACCAACC TTTGAATTAT TATCTGTTCC AATATATTAG 660ATGAGGGGTG TATCGGAATT TGACTTCCGA GTTGTTCTTG CGTGTCCGTA CGCTCGTACG 720GTAGCTCGTT GGGTTGTTGT ACCAGCCATC CTGCTACTGC GCAACGAAAT GCATTGGTCA 7 80TCTCAATTAA GTCCAAAGAT TGTAGTCGTA AACATGCAGC CAATAAGAGC AAGGATAATA 840GTTTAGCCAT TGATATGTCT TCTAAAGCTA ATTATTACTG TATTGGACCC ACCTCGTACT 900CTCATTCTCT CACCACTTGT TTCGGAATCT GTACTGCTAC AACCAGCTCT TAGTCGACTG 960ATAATTAACT ACCCGCTTTT TCTCTTCTCT CCCCTCCAAC TGCAAAAATC TAATGTGGCA 1020AACCATTTAG CCTGCTTACA TCGTCAAAAA TCTAATGTGG TAAAGTGTGA AGTGTCCTAA 1080AGTTTTAGTC CTTAATTTCT TTCAATAAAC TAAACTAAAC TTTAGAAAAC TCAAACAAGT 1140CCTCATGTTT GCACATTTTA GGTCTCGTTT GGTTTGAGGG ACTAAAGATT AGTCCCTCCA 1200TTTTAGTCCC ATTTAGTTAC TAAATTACCA AACAGTAGGA CTAAAACAGG GACTAAATTG 12 60TTTTAGTCCC TAGTCCCTTA AGATGGCTAA AAGGGACTAA ACCATATTAA TTCCACATTT 1320GCCCCTCATT TAGTTCAATT GTACTAATAG CAGGAGAATG TTAAAAGTCA TTTTAATCTT 1380CTTATGAGTC ATTTAGGCCC TGTTTGGTTC CATTAGTCAT AGAACTAAAG TTTAGTTGTA 14 4 0GGGACTAAAT AGATTCTAAA TACATTAAAT GCAACACATA AAGACCAAAA TGCCCTTTTT 1500TGTTTGACAC CCCTTTTCTA GCAAGGGTAT TTGGAGTAAA TGTTGCCCTT TGGTCCCTTT 1560TAGCACCCAT GTGAGGGACT AGAGACTAAA ACCAATTAGT CCCTACTTTA GTCATTCCGT 1620TTAGCAAAAT AGAGACTAAA CGAGACTAAA AACGAGAGGC TAAAGATTAG TCTCTCTAAC 1680CAAACGGGAC CTAAAATTAC TATCTGTATG TATCTGTTGG ATGGAAAAGT CAGAACGTCG 17 4 0TGGGGACCAC CACGCTACCA CATGGTACGG TAATGTCAGA AAGTCGCTAT CTTCTTCGAT 1800CTGCATCTCC ACTCCAGCCA GCGCTGCTTA TCATCAGCAT TCACGAAGCC GCCCAACGAT 1860AATAAAAAAT GTCAGCGCGA TCGCGCACTG CCTATAAAAC CCCGGCCGTC GCGTCCATGG 1920CGTTTCAGGA TCCGAGCACC AGAAAGAAGC TGAGTTAGCT AGGGTCAAGA AAGTAGTCAG 1980CACTCAGCAG GAAAAGAAGC AGAGACTACA CATC 2014

<210> 38

<211> 1014

<212> DNA

<213> Zea mays<400> 38

TGCAAAAATC TAATGTGGCA AACCATTTAG CCTGCTTACA TCGTCAAAAA TCTAATGTGG 60

TAAAGTGTGA AGTGTCCTAA AGTTTTAGTC CTTAATTTCT TTCAATAAAC TAAACTAAAC 120

TTTAGAAAAC TCAAACAAGT CCTCATGTTT GCACATTTTA GGTCTCGTTT GGTTTGAGGG 180

ACTAAAGATT AGTCCCTCCA TTTTAGTCCC ATTTAGTTAC TAAATTACCA AACAGTAGGA 240

CTAAAACAGG GACTAAATTG TTTTAGTCCC TAGTCCCTTA AGATGGCTAA AAGGGACTAA 300

ACCATATTAA TTCCACATTT GCCCCTCATT TAGTTCAATT GTACTAATAG CAGGAGAATG 360

TTAAAAGTCA TTTTAATCTT CTTATGAGTC ATTTAGGCCC TGTTTGGTTC CATTAGTCAT 420

AGAACTAAAG TTTAGTTGTA GGGACTAAAT AGATTCTAAA TACATTAAAT GCAACACATA 480

AAGACCAAAA TGCCCTTTTT TGTTTGACAC CCCTTTTCTA GCAAGGGTAT TTGGAGTAAA 540

TGTTGCCCTT TGGTCCCTTT TAGCACCCAT GTGAGGGACT AGAGACTAAA ACCAATTAGT 600

CCCTACTTTA GTCATTCCGT TTAGCAAAAT AGAGACTAAA CGAGACTAAA AACGAGAGGC 660

TAAAGATTAG TCTCTCTAAC CAAACGGGAC CTAAAATTAC TATCTGTATG TATCTGTTGG 720

ATGGAAAAGT CAGAACGTCG TGGGGACCAC CACGCTACCA CATGGTACGG TAATGTCAGA 780

AAGTCGCTAT CTTCTTCGAT CTGCATCTCC ACTCCAGCCA GCGCTGCTTA TCATCAGCAT 840

TCACGAAGCC GCCCAACGAT ΑΑΤΑΑΑΑΑΑΤ GTCAGCGCGA TCGCGCACTG CCTATAAAAC 900

CCCGGCCGTC GCGTCCATGG CGTTTCAGGA TCCGAGCACC AGAAAGAAGC TGAGTTAGCT 960AGGGTCAAGA AAGTAGTCAG CACTCAGCAG GAAAAGAAGC AGAGACTACA CATC 1014

<210> 39

<211> 18

<212> DNA

<213> PRIMER

<400> 39

CGGGGTTCGC CAGCCTCC 18

<210> 40

<211> 17

<212> DNA

<213> PRIMER

<400> 40

AGTGGGCTCC CTCTCCG 17

<210> 41

<211> 18

<212> DNA

<213> PRIMER

<400> 41

GCTCGTCATG CCGCTCGC

<210> 42

<211> 18

<212> DNA

<213> PRIMER

<400> 42

GCACTGGATG TCGGGCAT

<210> 43

<211> 20

<212> DNA

<213> PRIMER

<4 00> 43

AATTAACCCT CACTAAAGGG

<210> 44<211> 22<212> DNA

<213> PRIMER

<400> 44

GTAATACGAC TCACTATAGG GC 22

<210> 45

<211> 5812

<212> DNA

<213> Zea mays

<4 00> 45

GGTTGGCGAG CGGGTGTGGT CTGGGCAGTGGTGGGGGAGG GAGTGGCGAG AGAGGGAGGAGACGTACGTC GGCGCTTGTC AGGGTTTCGTTAAAGTAATG TTGGGAGTGT TTTGAAAAAACTTTAAGTAT AGTAGAGATT TAAAATTAAAAACTGCAGAT ATTACACTTT ATCTTAGCCAGAGACATGCC CTTTTATAAC TCACTCGGTCCTATTCGAGA ACGTTGTATT ACATGTGGTTACTAACTATC TGGGTGRTAA GATTGCTAGACTACACCGTT TCATGCGTGA CATGATATACAAATAAAGAT AGATAAACCA TAAATTACTACCAAGGAGGG CAAGGGCAAG ATGGCCGAGGGATGCAACAC ACTAGCTGTT CGGAGACAATTGAAACACAC AAGCTGTTAC AGTGGCTCTAGCATTCTTTA CGCAGGGCAA GAGTGTTATTTCTTTTTTTT TAATTGGTGC ATTTTCCTTAGTTCATCACG TCGTTGTGCC CTGGCACGTCGCTGAATGCG CACCACAGAC TCTTGGGCGAAGAGCGGCAG AGACGACAGA GATATGACGACGGAGTTTTA GATGTCTATT TCCACCCTGAGAATTTCTGC ATCTGCAATC ATTGGACCAGTTGCCCGGCC GAGATCCTCT GGGGTCAACCCCTGGGCCTC ACGGAGAGAC TCCTTCACGTGGCATCCGAC GCTCCTGGGC CCACTTGCCGCCCACGTAAT TAAAGTGTGA CTGGGTTAGTGATGCGACAA AACGGCCGCT AGATTGGATTTTCTGGAAGG TTGGTTAGCT CATGGAGTTGTGTTCACCTT CATATTTATC ATTCGTGTAAGGAGTTTTGT GTCAACCAAT AACCGATCAATAAAACACAT AATGTGTTCT AATTTTGTCTGCTTGAGGGT GGTGTTACGA CGAAAAACAAAATTAATCAA TCACATCGAT ATGCTAATGCAATGCAATGA GGTGATGGCA GGCAGCCGCAGTAGGCACGT ACAAAAGCCA CACGGACATGACACCGCTTG CCCTCCGCCT TCTCGTTCTCCCATCACCAC ACACATTTAA AACCACCAGCCCCACAGGTC TGGAACTAGT AGCCACTAGCTTCCTGCACA GCCACGAGGC CAGGCAGGCAAAAGCAGCAG CTGGCCGACG ACGAAGAGAAGGAGTGCGGC GTCGATGCCG AGTTCAGGGCGCCGCACACG CAGGCGTTCC ACCTCGCCTGCTTTGCCGCC CCGCCCATCC TCCCTGCGCTCGCCCCCGCC GCCGCAGTGG GCTCCCTCTCGCCCGCATGC GACCTCCTCG GCCCGCGCCGGCTCGCCGTC GCGGTCACCG CGGTCACCGCCTTCGTGGCG GGCCTCTCCC TCGCCAACTTCTTCGCGCCC TCCGCCGTGG GGCTCGCCAA

GCAATGGCGG GGGCAGCGAA GAGGAGGGCG 60AAGAGAGATG AGGCGTGTGC AACAACAGGA 120GCAATGAGAT ATGGGTGTGT GGGTTGATTC 180TTTGACGCAG GACGACCGTT GAAACTAGTG 24 0GTGGACACAT GGCCCACATA CTGAATATTA 300AAAGGTCGAG AAATGTATGA GTTAAAAAAG 360GCTTGTCCTA CTTCAACTAT TAAGTTTGTA 4 20TTGTGTCATA TTGGGTTTGG GTGTTTTCTC 4 80CGAGACGTAG AGGAGAAAAA CATATCTACT 54 0GAAACCCAAG TTTTAAAGGA GTAAAAATAA 600TCTACAAAAA CGTAGACAGC AGGCTAGATA 660CACTTGTGCC CGCCGGAGCT TTGGATGCAA 720CGGTGTATCA AAGAAGTAAA AAAATTTGGA 780GAGGAAAGAT TGGGATTTTC ATTTTCTGAT 84 0TCTGCTGATG TACACATAAT TAGAAGACTC 900TGAACCACAT GCGTAAAAAA CTGGGCCGAA 960ACCAATCGCA ACGCTCAGCT AGAAGCTGCT 1020AACCAGTTCA TCTGTTTTTT TTTTACGCGC 1080TGTATATTAT GGATTAATTA AAAAGCGATC 114 0GGAGCCAAAA AGGATTCATC GGAGATTCAG 1200AGCGGCGGTA GTATATTCCG ATCTACAGGC 1260TCGCTGCTAC GCGGGAGGGC GGGCGCAGCC 1320CTCCGGGCCC ACTACAGAAG GCCGAGTAGT 1380TCTCGAGTCA CCATACGCGC GGGCCCCCAG 14 4 0CCTGTCCGAG GCTAGCGCAG AGTGGGATGC 1500ATTAGTATAG AGAGTATACA GATTAGAGAG 1560ATCGATTCCC GCTCGTGTCA AACACGTATA 1620ATTCACGGAG AGTAATATAC ATTGCTTACT 1680AGATGTTGTT ATTTACTGCA TCCACACTAA 17 4 0TGGGKTAATT TTGTCCTGGA GATGACTTTA 1800TGCCGTATAG TTCTAAGGTT AGATTTTTGC 18 60TAAATTGCTA ATGCTATGCT TTAAATTGCT 1920GTCCCTTTTC ATGGCCTCGG GGAGCCGGTG 1980CAACGCGGCG CCCTGCATGC ACCCGCCGCG 204 0GGTCCACCAC CTTCTATTCC ATTTCCACAC 2100GAGTATCTAA ACCTTTCACC CCATTGGTCG 2160TCCATTCTCT GCTTGGCTGT GGTAGATCTC 2220GACGTCACTA GCTATGGTGG CGATGGGGAA 2280CTGCTGCTAC GGCGTCGGCA GCTCTGAGGC 234 0GACGGATCTG CGCCCTCTGT CACTGCTGTC 24 00GCTCTCCCTC TTCGCCTGCT TCTTCGCGGC 24 60GCGGCCGGCG CTCGTGCTCG CGCCCTCGGA 2520CGCCACGCTG GTCGGCAGGC TTGCCATGGG 2580CGCGTCGGGG TTCGCCAGCC TCCTGGCCGC 264 0GTCGTCGCCC GCGGGGTTCG TCGCGCTGCG 2700CGTCGCCAAC CAGCACTGGA TGTCGGGCAT 27 60CGCCGTCACG GCCGGCTGGG CCAACGTCGG 2820CAGCGCCGCG GCGCAGCTCG TCATGCCGCT CGCGTACGAG CTCGTCCTCC GCCTCGGCGT 2880GCCCATCACC GTCGCCTGGC GCGTCACCTA CCTCCTCCCC TGCGCGCTCC TCATCACCAC 2 94 0GGGCCTCGCC GTCCTCGCCT TCCCYTACGA CCTCCCGCGC GGCGCCGGCG TCGGCGGCGG 3000AGCCAAGACC GGCAAGAGCT TGTGGAAGGT GGTGCGCGGA GGGGTCAGCA ACTACCGCGC 3060GTGGGTGCTC GCGCTCACCT ACGGCTACTG CTACGGCGTC GAGCTCATCA TGGAGAACGT 3120GGCCGCCGAC TTCTTCCGGA AACGTTTCCA CCTCCCCATG GAGGCTGCGG GCGCCGCGGC 3180GGCGTGCTTC GGCGCGATGA ACGCGGTGGC GCGGCCCGCG GGCGGGTTGG CGTCGGACGC 3240GGTGGCGAGA CTGTTCGGCA TGCGCGGGAG GCTGTGGCTT CTCTGGGCCG TGCAGACCAC 3300CGGCGCGGCA CTGTGCGTGC TGGTCGGCAG GATGGGCGCA GCGGAAGCGC CGTCGCTGGC 3360GGCCACCATG GCGGTCATGG TGCTGTGCGC CGCGTTTGTG CAGGCCTCGT CGGGGCTCAC 3420CTTCGGCATC GTCCCGTTCG TGTCCAAGAG GTGAATCCAA CAAACTTCTT ACAACATCTA 34 80ATACAGATTA TTTTGCGTCG GATTAATTCA AAAATAGTTA TATATAGATT CTAAGTATAT 3540ATTCACATAT AGATTTTTTT TCCACCCAAA AAGTTATAAC TTACAAGGAA GGACATCTAT 3600CATGCATGTT TCATAAACAA ATTAACTAAA GATTTTTCTG TGTTTGGTTA TTTAGATATA 3660AATAGATCTT GAATTATATA TTGACGTACA GATCCCCTCC CTCAAAGTTA TAACGTAAAT 3720AATAAGGGCA AAGACGTTGA AGCTGATATA TACCTCTCAA TTGAAAGATG GCCACGCCAG 3780CTAGCTTTTT GAAGATATTT TCTAAGCACA CAAACACCTA ATTACTGCTC CGTTCATTTA 3840AAATTATAGC TTTAAAAATT AAAATCAAAG CGTTTAATTA GAAAAATCTA AAATTCTTCA 3900AGCTATAAGT TTAATTAGAA AAATCAAAAC ATTTAATAAT TTAAAATAGA TGAAACATAC 3960CCAACTAAGA GGGCCACATC GTTATCATAG GCCCTAATAT AGATTCTATA GTAGAATCCT 4 020GGTATACTAC TATTGTTGAT GTTCACCTGT TTTCTGATAT TTGTGGACGA AAATAATCAG 4 080AGAGGTTTCC AACAATAAAG CAACTCATTA ATTATTTCTC TGAACATATA GGAGGACGTG 4140TTTGGTTGCC ACGCTAGCCA TGTCCAAGCT CACGCGCGTG TACTTGGTTA TCTGCATGTA 4200ATTAACAAAG CGAACTCGCA CGCACGCGTA CAACCTAAGC ACCTTTTCCA CCTCCTACAT 4 2 60GCATATGTAG GGAAGCGGCC GGGTCCGCGC GAGTCAGGAG CTCTCAACTC ACAAACCAAT 4 320CACGTCCATA ACAACCAAGG ACTGTAAAAT GTGGCGTACA TATTTTTTAT GTCTAAGGGC 4 380TAGTTTGAGA CTCCATTATC CTAAGAGAAA GTGAATTAAT TAGATTCCTA AACTAGCCCT 4440GATATGAAAA AGAAACACCG GAAAAACTAC GGTAGCAAAA TAGCCAGTGG AAAATAAACT 4 500TGTCGTCACA AGTTACTCTT CTATTCCAAT ACCTCTTGTA TATGTATTTT AAAGACACGG 4 560CCTTAAACAT TTTTTTTAAA AAAAAAAAAT CCATCTAATG AATTAGCCTA GGAATATCAT 4 620GCATGGTTTT CTCAAAATAA TGTCTTCGAC CCCATTTGGT CACAAATTAA TTTATCTAAA 4 680CTAGATCTAA CTCGTAGCAT GAGTTTTAGA GCGCCAGAGG CAATTTGTTA TTACAGAAAG 4 74 0ATTAAGGTCA TGTTTGATAC ACTTCAGCTT TACAGGTGAA GGTGTTTTAA AAAAAAATAA 4 800CTTCACCAAT AACGATTGGA GAAGGAAATG AGGAAGAAAG CTACCCAAAG TTACTTTTTC 4 8 60GGCTTCACCT CTGTCTAATT CTGCGTCTGA GCATAAAAAG GAGTTTTACC TATGAATCTT 4 920TTTGAAAAAA AAGAATGTTT ACAAAAAAAT AAATAGCTCA ACAACTTATA AAGCTTCTGA 4980TTAATCTGTA CTAAAAAAGA ACTAACTATA AACAAAGGTC AAAGAAACCA TGACACATTT 5040CTTACGGCTT GTGTTGGGTC ACTTAATTTC GGTGGTGTGT GTGCAGGTCG TTGGGCGTGG 5100TGTCCGGGAT GACGGCGAGC GGCGGCGCGG TGGGCGCGAT CGTGACGAAC CGGCTCTTCT 5160TCAGCGGGTC GCGGTACACC ATTGAGGAGG CYATCTCGTT GACCGGCGCC GCCAGCCTCG 5220TGTGCACGCT CCCGCTGGCC CTCGTCCACT TCCCGCGCCA CGGTGGCATG CTCTGCGGCC 5280CAACCGCCGT CGTCGATGGC GACGATGCAG GATACGACAA CGATAATAGT GCTGGAGATT 5340ACACGCTCCT CAAATGAATT GAGGAACAAA TGTATGCAAC GGGGGGGTCG CATGTGAACT 54 00TTGTACATAG CACATCCAAT GGCCTTGATA GATTAGCAAA CGATTACTCA TGGTTTGTTT 54 60CAGGATCAGG GGTGCGATAT GAGCGACACA CGGATAGAAA TATGTCGAGT GGCTTCGTCT 5520GTCGATCACC TGCACATAAA TAGATAGAGA GTAGAGATGG CTCGTAGGTT GTTCACGTGT 5580CGCTGCCGCA TTGGCAATTG CGTGTCTTAT GTTTGTGTTG GTTCGAAGAG TGAGACAATA 564 0ATAAGTTGTC GGTGTTCGAA TCAGTACCAA CGAGTAAATT GTGTATGCGT GCATGTTTTG 5700GATTTGGATG ATGTGTTCAG TGAACGCAAG ATTTATACTG ATTCGGATAG AACGTCCCTA 5760CTTCTAGTCT TCGATGGCTC GCGTAATCGA TAACTTCTTG CTGAATGCTC AT 5812

<210> 46

<211> 2263

<212> DNA

<213> Zea mays

<400> 46

GGTTGGCGAG CGGGTGTGGT CTGGGCAGTG GCAATGGCGG GGGCAGCGAA GAGGAGGGCG 60GTGGGGGAGG GAGTGGCGAG AGAGGGAGGA AAGAGAGATG AGGCGTGTGC AACAACAGGA 120GACGTACGTC GGCGCTTGTC AGGGTTTCGTTAAAGTAATG TTGGGAGTGT TTTGAAAAAACTTTAAGTAT AGTAGAGATT TAAAATTAAAAACTGCAGAT ATTACACTTT ATCTTAGCCAGAGACATGCC CTTTTATAAC TCACTCGGTCCTATTCGAGA ACGTTGTATT ACATGTGGTTACTAACTATC TGGGTGRTAA GATTGCTAGACTACACCGTT TCATGCGTGA CATGATATACAAATAAAGAT AGATAAACCA TAAAT TAC TACCAAGGAGGG CAAGGGCAAG ATGGCCGAGGGATGCAACAC ACTAGCTGTT CGGAGACAATTGAAACACAC AAGCTGTTAC AGTGGCTCTAGCATTCTTTA CGCAGGGCAA GAGTGTTATTTCTTTTTTTT TAATTGGTGC ATTTTCCTTAGTTCATCACG TCGTTGTGCC CTGGCACGTCGCTGAATGCG CACCACAGAC TCTTGGGCGAAGAGCGGCAG AGACGACAGA GATATGACGACGGAGTTTTA GATGTCTATT TCCACCCTGAGAATTTCTGC ATCTGCAATC ATTGGACCAGTTGCCCGGCC GAGATCCTCT GGGGTCAACCCCTGGGCCTC ACGGAGAGAC TCCTTCACGTGGCATCCGAC GCTCCTGGGC CCACTTGCCGCCCACGTAAT TAAAGTGTGA CTGGGTTAGTGATGCGACAA AACGGCCGCT AGATTGGATTTTCTGGAAGG TTGGTTAGCT CATGGAGTTGTGTTCACCTT CATATTTATC ATTCGTGTAAGGAGTTTTGT GTCAACCAAT AACCGATCAATAAAACACAT AATGTGTTCT AATTTTGTCTGCTTGAGGGT GGTGTTACGA CGAAAAACAAAATTAATCAA TCACATCGAT ATGCTAATGCAATGCAATGA GGTGATGGCA GGCAGCCGCAGTAGGCACGT ACAAAAGCCA CACGGACATGACACCGCTTG CCCTCCGCCT TCTCGTTCTCCCATCACCAC ACACATTTAA AACCACCAGCCCCACAGGTC TGGAACTAGT AGCCACTAGCTTCCTGCACA GCCACGAGGC CAGGCAGGCA

GCAATGAGAT ATGGGTGTGT GGGTTGATTC 180TTTGACGCAG GACGACCGTT GAAACTAGTG 240GTGGACACAT GGCCCACATA CTGAATATTA 300AAAGGTCGAG AAATGTATGA GTTAAAAAAG 360GCTTGTCCTA CTTCAACTAT TAAGTTTGTA 420TTGTGTCATA TTGGGTTTGG GTGTTTTCTC 4 80CGAGACGTAG AGGAGAAAAA CATATCTACT 54 0GAAACCCAAG TTTTAAAGGA GTAAAAATAA 600TCTACAAAAA CGTAGACAGC AGGCTAGATA 660CACTTGTGCC CGCCGGAGCT TTGGATGCAA 720CGGTGTATCA AAGAAGTAAA AAAATTTGGA 780GAGGAAAGAT TGGGATTTTC ATTTTCTGAT 84 0TCTGCTGATG TACACATAAT TAGAAGACTC 900TGAACCACAT GCGTAAAAAA CTGGGCCGAA 960ACCAATCGCA ACGCTCAGCT AGAAGCTGCT 1020AACCAGTTCA TCTGTTTTTT TTTTACGCGC 1080TGTATATTAT GGATTAATTA AAAAGCGATC 114 0GGAGCCAAAA AGGATTCATC GGAGATTCAG 1200AGCGGCGGTA GTATATTCCG ATCTACAGGC 1260TCGCTGCTAC GCGGGAGGGC GGGCGCAGCC 1320CTCCGGGCCC ACTACAGAAG GCCGAGTAGT 1380TCTCGAGTCA CCATACGCGC GGGCCCCCAG 14 4 0CCTGTCCGAG GCTAGCGCAG AGTGGGATGC 1500ATTAGTATAG AGAGTATACA GATTAGAGAG 1560ATCGATTCCC GCTCGTGTCA AACACGTATA 1620ATTCACGGAG AGTAATATAC ATTGCTTACT 1680AGATGTTGTT ATTTACTGCA TCCACACTAA 174 0TGGGKTAATT TTGTCCTGGA GATGACTTTA 1800TGCCGTATAG TTCTAAGGTT AGATTTTTGC 18 60TAAATTGCTA ATGCTATGCT TTAAATTGCT 1920GTCCCTTTTC ATGGCCTCGG GGAGCCGGTG 1980CAACGCGGCG CCCTGCATGC ACCCGCCGCG 204 0GGTCCACCAC CTTCTATTCC ATTTCCACAC 2100GAGTATCTAA ACCTTTCACC CCATTGGTCG 2160TCCATTCTCT GCTTGGCTGT GGTAGATCTC 2220GACGTCACTA GCT 2263

<210> 47

<211> 1263

<212> DNA

<213> Zea mays

<400> 47

ACGCTCAGCT AGAAGCTGCT GCTGAATGCGTCTGTTTTTT TTTTACGCGC AGAGCGGCAGGGATTAATTA AAAAGCGATC CGGAGTTTTAAGGATTCATC GGAGATTCAG GAATTTCTGCGTATATTCCG ATCTACAGGC TTGCCCGGCCGCGGGAGGGC GGGCGCAGCC CCTGGGCCTCACTACAGAAG GCCGAGTAGT GGCATCCGACCCATACGCGC GGGCCCCCAG CCCACGTAATGCTAGCGCAG AGTGGGATGC GATGCGACAAAGAGTATACA GATTAGAGAG TTCTGGAAGGGCTCGTGTCA AACACGTATA TGTTCACCTTAGTAATATAC ATTGCTTACT GGAGTTTTGTATTTACTGCA TCCACACTAA TAAAACACATTTGTCCTGGA GATGACTTTA GCTTGAGGGTTTCTAAGGTT AGATTTTTGC AATTAATCAAATGCTATGCT TTAAATTGCT AATGCAATGA

CACCACAGAC TCTTGGGCGA AACCAGTTCA 60

AGACGACAGA GATATGACGA TGTATATTAT 120

GATGTCTATT TCCACCCTGA GGAGCCAAAA 180

ATCTGCAATC ATTGGACCAG AGCGGCGGTA 240

GAGATCCTCT GGGGTCAACC TCGCTGCTAC 300

ACGGAGAGAC TCCTTCACGT CTCCGGGCCC 360

GCTCCTGGGC CCACTTGCCG TCTCGAGTCA 4 20

TAAAGTGTGA CTGGGTTAGT CCTGTCCGAG 4 80

AACGGCCGCT AGATTGGATT ATTAGTATAG 540

TTGGTTAGCT CATGGAGTTG ATCGATTCCC 600

CATATTTATC ATTCGTGTAA ATTCACGGAG 660

GTCAACCAAT AACCGATCAA AGATGTTGTT 720

AATGTGTTCT AATTTTGTCT TGGGKTAATT 780

GGTGTTACGA CGAAAAACAA TGCCGTATAG 84 0

TCACATCGAT ATGCTAATGC TAAATTGCTA 900

GGTGATGGCA GGCAGCCGCA GTCCCTTTTC 960ATGGCCTCGG GGAGCCGGTG GTAGGCACGTCCCTGCATGC ACCCGCCGCG ACACCGCTTGCTTCTATTCC ATTTCCACAC CCATCACCACACCTTTCACC CCATTGGTCG CCCACAGGTCGCTTGGCTGT GGTAGATCTC TTCCTGCACAGCT

ACAAAAGCCA CACGGACATG CAACGCGGCG 1020CCCTCCGCCT TCTCGTTCTC GGTCCACCAC 1080ACACATTTAA AACCACCAGC GAGTATCTAA 1140TGGAACTAGT AGCCACTAGC TCCATTCTCT 1200GCCACGAGGC CAGGCAGGCA GACGTCACTA 1260

1263

<210> 48

<211> 1455

<212> DNA

<213> Zea mays

<400> 48

ATGGTGGCGA TGGGGAAAAA GCAGCAGCTGGTCGGCAGCT CTGAGGCGGA GTGCGGCGTCCCTCTGTCAC TGCTGTCGCC GCACACGCAGGCCTGCTTCT TCGCGGCCTT TGCCGCCCCGGTGCTCGCGC CCTCGGACGC CCCCGCCGCCGGCAGGCTTG CCATGGGGCC CGCATGCGACGCCAGCCTCC TGGCCGCGCT CGCCGTCGCGGGGTTCGTCG CGCTGCGCTT CGTGGCGGGCCACTGGATGT CGGGCATCTT CGCGCCCTCCGGCTGGGCCA ACGTCGGCAG CGCCGCGGCGGTCCTCCGCC TCGGCGTGCC CATCACCGTCGCGCTCCTCA TCACCACGGG CCTCGCCGTCGCCGGCGTCG GCGGCGGAGC CAAGACCGGCGTCAGCAACT ACCGCGCGTG GGTGCTCGCGCTCATCATGG AGAACGTGGC CGCCGACTTCGCTGCGGGCG CCGCGGCGGC GTGCTTCGGCGGGTTGGCGT CGGACGCGGT GGCGAGACTGTGGGCCGTGC AGACCACCGG CGCGGCACTGGAAGCGCCGT CGCTGGCGGC CACCATGGCGGCCTCGTCGG GGCTCACCTT CGGCATCGTCGTGTCCGGGA TGACGGCGAG CGGCGGCGCGTTCAGCGGGT CGCGGTACAC CATTGAGGAGGTGTGCACGC TCCCGCTGGC CCTCGTCCACCCAACCGCCG TCGTCGATGG CGACGATGCATACACGCTCC TCAAA

<210> 49

<211> 485

<212> PRT

<213> Zea mays

GCCGACGACG AAGAGAACTG CTGCTACGGC 60GATGCCGAGT TCAGGGCGAC GGATCTGCGC 120GCGTTCCACC TCGCCTGGCT CTCCCTCTTC 180CCCATCCTCC CTGCGCTGCG GCCGGCGCTC 240GCAGTGGGCT CCCTCTCCGC CACGCTGGTC 300CTCCTCGGCC CGCGCCGCGC GTCGGGGTTC 360GTCACCGCGG TCACCGCGTC GTCGCCCGCG 420CTCTCCCTCG CCAACTTCGT CGCCAACCAG 480GCCGTGGGGC TCGCCAACGC CGTCACGGCC 540CAGCTCGTCA TGCCGCTCGC GTACGAGCTC 600GCCTGGCGCG TCACCTACCT CCTCCCCTGC 660CTCGCCTTCC CYTACGACCT CCCGCGCGGC 720AAGAGCTTGT GGAAGGTGGT GCGCGGAGGG 780CTCACCTACG GCTACTGCTA CGGCGTCGAG 840TTCCGGAAAC GTTTCCACCT CCCCATGGAG 900GCGATGAACG CGGTGGCGCG GCCCGCGGGC 960TTCGGCATGC GCGGGAGGCT GTGGCTTCTC 1020TGCGTGCTGG TCGGCAGGAT GGGCGCAGCG 1080GTCATGGTGC TGTGCGCCGC GTTTGTGCAG 1140CCGTTCGTGT CCAAGAGGTC GTTGGGCGTG 1200GTGGGCGCGA TCGTGACGAA CCGGCTCTTC 1260GCYATCTCGT TGACCGGCGC CGCCAGCCTC 1320TTCCCGCGCC ACGGTGGCAT GCTCTGCGGC 1380GGATACGACA ACGATAATAG TGCTGGAGAT 1440

1455

<400> 49

MET VAL ALA MET GLY LYS LYS GLN1 5

CYS CYS TYR GLY VAL GLY SER SER20

GLU PHE ARG ALA THR ASP LEU ARG35 40

GLN LEU ALA ASP ASP GLU GLU ASN10 15

GLU ALA GLU CYS GLY VAL ASP ALA25 30

PRO LEU SER LEU LEU SER PRO HIS45

THR GLN ALA PHE HIS LEU ALA TRP LEU SER LEU PHE ALA CYS PHE PHE50 55 60ALA ALA PHE ALA ALA PRO PRO ILE LEU PRO ALA LEU ARG PRO ALA LEU65 70 75 80

VAL LEU ALA PRO SER ASP ALA PRO ALA ALA ALA VAL GLY SER LEU SER85 90 95

ALA THR LEU VAL GLY ARG LEU ALA MET GLY PRO ALA CYS ASP LEU LEU100 105 110

GLY PRO ARG ARG ALA SER GLY PHE ALA SER LEU LEU ALA ALA LEU ALAH5 120 125

VAL ALA VAL THR ALA VAL THR ALA SER SER PRO ALA GLY PHE VAL ALA130 135 140

LEU ARG PHE VAL ALA GLY LEU SER LEU ALA ASN PHE VAL ALA ASN GLN145 150 155 160

HIS TRP MET SER GLY ILE PHE ALA PRO SER ALA VAL GLY LEU ALA ASN165 170 175

ALA VAL THR ALA GLY TRP ALA ASN VAL GLY SER ALA ALA ALA GLN LEU180 185 190

VAL MET PRO LEU ALA TYR GLU LEU VAL LEU ARG LEU GLY VAL PRO ILE195 200 205

THR VAL ALA TRP ARG VAL THR TYR LEU LEU PRO CYS ALA LEU LEU ILE210 215 220

THR THR GLY LEU ALA VAL LEU ALA PHE PRO TYR ASP LEU PRO ARG GLY225 230 235 240

ALA GLY VAL GLY GLY GLY ALA LYS THR GLY LYS SER LEU TRP LYS VAL245 250 255

VAL ARG GLY GLY VAL SER ASN TYR ARG ALA TRP VAL LEU ALA LEU THR260 265 270

TYR GLY TYR CYS TYR GLY VAL GLU LEU ILE MET GLU ASN VAL ALA ALA275 280 285

ASP PHE PHE ARG LYS ARG PHE HIS LEU PRO MET GLU ALA ALA GLY ALA290 295 300

ALA ALA ALA CYS PHE GLY ALA MET ASN ALA VAL ALA ARG PRO ALA GLY305 310 315 320

GLY LEU ALA SER ASP ALA VAL ALA ARG LEU PHE GLY MET ARG GLY ARG325 330 335

LEU TRP LEU LEU TRP ALA VAL GLN THR THR GLY ALA ALA LEU CYS VAL340 345 350

LEU VAL GLY ARG MET GLY ALA ALA GLU ALA PRO SER LEU ALA ALA THR355 360 365

MET ALA VAL MET VAL LEU CYS ALA ALA PHE VAL GLN ALA SER SER GLY370 375 380LEU THR PHE GLY ILE VAL PRO PHE VAL SER LYS ARG SER LEU GLY VAL385 390 395 400

VAL SER GLY MET THR ALA SER GLY GLY ALA VAL GLY ALA ILE VAL THR405 410 415

ASN ARG LEU PHE PHE SER GLY SER ARG TYR THR ILE GLU GLU ALA ILE420 425 430

SER LEU THR GLY ALA ALA SER LEU VAL CYS THR LEU PRO LEU ALA LEU435 440 445

VAL HIS PHE PRO ARG HIS GLY GLY MET LEU CYS GLY PRO THR ALA VAL450 455 460

VAL ASP GLY ASP ASP ALA GLY TYR ASP ASN ASP ASN SER ALA GLY ASP465 470 475 480

TYR THR LEU LEU LYS485

<210> 50<211> 14<212> PRT<213> Zea mays

<220>

<221> DOMÍNIO

<222> (1)..(14)

<223> XAA=QUALQUER AMINOÁCIDO

<220>

<221> CARACTERÍSTICA MISC.<222> (6)..(6)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.<222> (7) .. (7)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<4 00> 50

ARG LEU ALA MET GLY XAA XAA CYS ASP LEU LEU GLY PRO ARG15 10

<210> 51

<211> 28

<212> PRT

<213> Zea mays

<220>

<221> DOMÍNIO

<222> (1)..(28)

<223> XAA=QUALQUER AMINOÁCIDO

<220>

<221> CARACTERÍSTICA MISC.<222> (4) .. (4)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (5)..(5)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (10)..(10)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (16)..(16)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (19)..(19)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (21)..(21)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (22)..(22)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<220>

<221> CARACTERÍSTICA MISC.

<222> (25)..(25)

<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL

<4 00> 51

THR PHE GLY XAA XAA PRO PHE VAL SER XAA ARG SER LEU GLY VAL XAA SER GLY XAA

15 10 15

THR XAA XAA GLY GLY XAA VAL GLY ALA

20 25<210> 52<211> 11<212> PRT<213> Zea mays<220> <221> DOMÍNIO<222> (1) ·· (14)<223> XAA=QUALQUER AMINOÁCIDO<220> <221> CARACTERÍSTICA MISC.<222> (6) . . (6)<223> XAA PODE SER QUALQUER AMINOÁCIDO DE OCORRÊNCIA NATURAL<400> 52

CYS THR LEU PRO LEU XAA LEU VAL HIS PHE PRO1 5 10

<210> 53

<211> 1561

<212> DNA

<213> Zea mays

<400> 53

TAGCTATATA CACATGTCTG GTCTGACGACCTTCCTATCA CTGTCATGAC ATGTGCTCTGTCGCTAATTC TTTCTTGTGC TAGAGGCGAGATCTGCGCTA AGGGTCACAT GACCCTGTTCCCTGACAGAT ACTTACGACG CGTCCGTACGCACCGGCGCC GGCATTCGCC CCCTGCCAGCCGCAGTTGCC GCATCCCAAA CGCCCGGGAATTGCTGCCCC TGGCTTCGTA AAGCTCTGACTCGCTCATAG GCCGACACGG CCGCAAAGTCGCCACGCGCC TATATATGTT CGCGGCCATGAACCCCGAGC TCAGATCCCT CGCCTCGTGTAGTGTGGGCC AGACGGACAC CGCCGAGCTACGGCCGGACC GGAGCACGTA CGTACGTACCGCCTTGTGTG TGCTGGCGGC GCTTCTCTTCGGGGTGCACC TCTCCTCGCT GCCCAAAGCGCAAGTCCTGC ACGCCGGCGT GGACTCGCTGCCGGCCGGCG CCGACGCCGG GTACAAGGGCAGCCAGAAGG ACCGCGGGTG GCGCAAGTCCCAGTTCAAGG TCACCGAGCA GGCGTACGCGGTCGCCCGCG ACGTCCCCTC GGGCTCCTACGGCGCCGAGG TGGCCTACGG CCAGACGGCGACCGGCATCC ACGCCTCTCT CAAGATCGCCGCGCTCGCCT TCTTCTTCGT CATCGAGACCCTGCGCGCCA TACATGCATA CATGTAAATCATTCATTGGT GCGCGCGACT ATTTTGGTGTCGTCAAAATT ACCAAATAAT AACTTAAGTTA

<210> 54<211> 612<212> DNA<213> Zea mays

AATCAAAAGG GATCGCTAGC TCGGGCTAGC 60CCTCTGCTGG TTGATAAGCC GTGCGCCTTC 120TCAAACAAAC GCTGCACCTC GTAGCCCTTA 180CCTATCGCTA GTTACCAACG ACCCATTCCC 24 0CGGCAGGCCT CGGCAGTTCG GCATCACCAG 300CGGTTCGCAG ATTCGCAGGG CGGAGTCGGC 360CCTTTGGGGC CCCTCTACGA GCAAATGAAG 4 20TTTTGATCAC TTGATTGGCA GTCGTACTCC 4 80AACTACCCGC TCCGCCATCC TTCAACCCCC 54 0TCCGTACTAG TCCTCCAACC CACAAGCCAC 600CGTGTCTCCG GTCGACGACG ACCAACAGCC 660TAGCGCTTGG TGATAGCAAG GGACGACCGG 720GCAGCGATGG CTCGGCAGCA AAGCGTGCAG 7 80GCCGCCTCCC TGCCGTCGCC GGCCGCCGCG 84 0CTCGACGTCA CCACCTCCGC CAAACCCGGC 900ACGGTGACGT GGAGCCTGAA CGCCACGGAG 960GTGAAGGTGA AGCTGTGCTA CGCGCCGGCG 1020GAGGACGACA TCAGCAAGGA CAAGGCGTGC 1080GCGGCGGCGC CCGGCAGCTT CCAGTACGCC 114 0TACCTGCGCG CCTTCGCCAC GGACGCGTCG 1200CCCACCGCCG CCTTCGACGT CGCCGGCATC 1260GCCGGCGTCT TCTCGGCCTT CTCCGTCGTC 1320CGCAAGAAGA ACAAGTAGAA CGAGTTGCGG 1380GTCGGCGGCG ATGAGTGGCT GTCGTTGCTG 1440ATCATGTAAG TTACTTTTCT GCAGTGTGTG 1500TCTCTGCTAA AAAAAAAAAA AAAAAAAAAA 1560

1561

<400> 54

ATGGCTCGGC AGCAAAGCGT GCAGGCCTTGTCCCTGCCGT CGCCGGCCGC CGCGGGGGTGGTCACCACCT CCGCCAAACC CGGCCAAGTCACGTGGAGCC TGAACGCCAC GGAGCCGGCCGTGAAGCTGT GCTACGCGCC GGCGAGCCAGGACATCAGCA AGGACAAGGC GTGCCAGTTCGCGCCCGGCA GCTTCCAGTA CGCCGTCGCCCGCGCCTTCG CCACGGACGC GTCGGGCGCCGCCGCCTTCG ACGTCGCCGG CATCACCGGCGTCTTCTCGG CCTTCTCCGT CGTCGCGCTCAAGAACAAGT AG

TGTGTGCTGG CGGCGCTTCT CTTCGCCGCC 60

CACCTCTCCT CGCTGCCCAA AGCGCTCGAC 120

CTGCACGCCG GCGTGGACTC GCTGACGGTG 180

GGCGCCGACG CCGGGTACAA GGGCGTGAAG 24 0

AAGGACCGCG GGTGGCGCAA GTCCGAGGAC 300

AAGGTCACCG AGCAGGCGTA CGCGGCGGCG 360

CGCGACGTCC CCTCGGGCTC CTACTACCTG 420

GAGGTGGCCT ACGGCCAGAC GGCGCCCACC 4 80

ATCCACGCCT CTCTCAAGAT CGCCGCCGGC 54 0

GCCTTCTTCT TCGTCATCGA GACCCGCAAG 600

612<210> 55

<211> 203

<212> PRT

<213> Zea mays

<400> 55

MET ALA ARG GLN GLN SER VAL GLN ALA LEU CYS VAL LEU ALA ALA LEU1 5 10 15

LEU PHE ALA ALA SER LEU PRO SER PRO ALA ALA ALA GLY VAL HIS LEU20 25 30

SER SER LEU PRO LYS ALA LEU ASP VAL THR THR SER ALA LYS PRO GLY35 40 45

GLN VAL LEU HIS ALA GLY VAL ASP SER LEU THR VAL THR TRP SER LEU50 55 60

ASN ALA THR GLU PRO ALA GLY ALA ASP ALA GLY TYR LYS GLY VAL LYS65 70 75 80

VAL LYS LEU CYS TYR ALA PRO ALA SER GLN LYS ASP ARG GLY TRP ARG85 90 95

LYS SER GLU ASP ASP ILE SER LYS ASP LYS ALA CYS GLN PHE LYS VAL100 105 110

THR GLU GLN ALA TYR ALA ALA ALA ALA PRO GLY SER PHE GLN TYR ALA115 120 125

VAL ALA ARG ASP VAL PRO SER GLY SER TYR TYR LEU ARG ALA PHE ALA130 135 140

THR ASP ALA SER GLY ALA GLU VAL ALA TYR GLY GLN THR ALA PRO THR145 150 155 160

ALA ALA PHE ASP VAL ALA GLY ILE THR GLY ILE HIS ALA SER LEU LYS165 170 175

ILE ALA ALA GLY VAL PHE SER ALA PHE SER VAL VAL ALA LEU ALA PHE180 185 190

PHE PHE VAL ILE GLU THR ARG LYS LYS ASN LYS195 200

<210> 56

<211> 756

<212> DNA

<213> Zea mays

<4 00> 56

TAGCTATATA CACATGTCTG GTCTGACGACCTTCCTATCA CTGTCATGAC ATGTGCTCTGTCGCTAATTC TTTCTTGTGC TAGAGGCGAGATCTGCGCTA AGGGTCACAT GACCCTGTTCCCTGACAGAT ACTTACGACG CGTCCGTACGCACCGGCGCC GGCATTCGCC CCCTGCCAGCCGCAGTTGCC GCATCCCAAA CGCCCGGGAATTGCTGCCCC TGGCTTCGTA AAGCTCTGAC

AATCAAAAGG GATCGCTAGC TCGGGCTAGC 60

CCTCTGCTGG TTGATAAGCC GTGCGCCTTC 120

TCAAACAAAC GCTGCACCTC GTAGCCCTTA 180

CCTATCGCTA GTTACCAACG ACCCATTCCC 24 0

CGGCAGGCCT CGGCAGTTCG GCATCACCAG 300

CGGTTCGCAG ATTCGCAGGG CGGAGTCGGC 360

CCTTTGGGGC CCCTCTACGA GCAAATGAAG 4 20

TTTTGATCAC TTGATTGGCA GTCGTACTCC 4 80TCGCTCATAG GCCGACACGG CCGCAAAGTCGCCACGCGCC TATATATGTT CGCGGCCATGAACCCCGAGC TCAGATCCCT CGCCTCGTGTAGTGTGGGCC AGACGGACAC CGCCGAGCTACGGCCGGACC GGAGCACGTA CGTACGTACC

AACTACCCGC TCCGCCATCC TTCAACCCCC 540

TCCGTACTAG TCCTCCAACC CACAAGCCAC 600

CGTGTCTCCG GTCGACGACG ACCAACAGCC 660

TAGCGCTTGG TGATAGCAAG GGACGACCGG 720

GCAGCG 7 56

<210> 57

<211> 594

<212> DNA

<213> Zea mays

<4 00> 57

ATGACGATGG CTCGTCCTGG GGCGGCTTTGTGCGCGCGCC TGGCGGCGGC AGTGCACCTCGCGTCGCCGA AGGCCGGACA AGTCCTGCACCACCTCAACG CGTCGGCGTC CAGCGTCGGGGCGCCGGCGA GCCAGGAGGA CCGCGGGTGGAAGGCGTGCC AGTTCAGGAT CGCCCGGCATTACAGGGTCG CCCGCGACGT CCCCACCGCGGCGTCCGGGG CGCCGGTGGG CTACGGCCAGGGCGTCTCGG GCGTCCACGC GTCCCTCCGGATCGCCGCGC TCGCCTTCTT TGTCGTCGTC

CCGCTGCTGC TGGTCGTGGT CGGCGCTTGC 60

TCCGCGCTCG GCAGGACACT CATCGTCGAG 120

GCCGGCGAGG ACACGATAAC CGTGACATGG 180

TACAAGGCGC TGGAGGTGAC CCTCTGCTAC 240

CGCAAGGCCA ACGACGACTT GAGCAAGGAC 300

GCATACGCCG GCGGCCAGGG GACGCTCCGG 360

TCCTACCACG TGCGCGCCTA CGCGCTGGAC 4 20

ACCGCGCCCG CCTACTACTT CCACGTCGCG 4 80

GTCGCCGCCG CCGTGCTCTC CGCGTTCTCC 54 0

GAGAAGAGGA GGAAGGACGA GTAG 594

<210> 58

<211> 197

<212> PRT

<213> Zea mays

<4 00> 58

MET THR MET ALA ARG PRO GLY ALA ALA LEU PRO LEU LEU LEU VAL VAL15 10 15

VAL GLY ALA CYS CYS ALA ARG LEU ALA ALA ALA VAL HIS LEU SER ALA20 25 30

LEU GLY ARG THR LEU ILE VAL GLU ALA SER PRO LYS ALA GLY GLN VAL35 40 45

LEU HIS ALA GLY GLU ASP THR ILE THR VAL THR TRP HIS LEU ASN ALA50 55 60

SER ALA SER SER VAL GLY TYR LYS ALA LEU GLU VAL THR LEU CYS TYR65 70 75 80

ALA PRO ALA SER GLN GLU ASP ARG GLY TRP ARG LYS ALA ASN ASP ASP85 90 95

LEU SER LYS ASP LYS ALA CYS GLN PHE ARG ILE ALA ARG HIS ALA TYR100 105 110

ALA GLY GLY GLN GLY THR LEU ARG TYR ARG VAL ALA ARG ASP VAL PRO115 120 125

THR ALA SER TYR HIS VAL ARG ALA TYR ALA LEU ASP ALA SER GLY ALA130 135 140

PRO VAL GLY TYR GLY GLN THR ALA PRO ALA TYR TYR PHE HIS VAL ALA145 150 155 160GLY VAL SER GLY VAL HIS ALA SER LEU ARG VAL ALA ALA ALA VAL LEU165 170 175

SER ALA PHE SER ILE ALA ALA LEU ALA PHE PHE VAL VAL VAL GLU LYS180 185 190

ARG ARG LYS ASP GLU195

<210> 59

<211> 30

<212> DNA

<213> PRIMER

<400> 59

GGTCGTTGGT AACTAGCGAT AGGGAACAGG 30

<210> 60

<211> 27

<212> DNA

<213> PRIMER

<400> 60

GTGCAGCGTT TGTTTGACTC GCCTCTA 27

<210> 61

<211> 18

<212> DNA

<213> PRIMER

<400> 61

CAACGGACCA GCTCTTGG 18

<210> 62

<211> 20

<212> DNA

<213> PRIMER

<400> 62

TCTTTGTGGG TTGTGGAAGG 20

<210> 63

<211> 20

<212> DNA

<213> PRIMER

<400> 63

CGAGCAGATC GTGCAAATAG 20

<210> 64

<211> 22

<212> DNA

<213> PRIMER

<400> 64

GGGCTTTGAT ATGTTTAGTT GG 22

<210><211>

65

2917<212> DNA

<213> Zea mays

<220>

<221> CARACTERÍSTICA MISC.

<222> (517)..(517)

<223> N É Α, C, G, OU T

<220>

<221> CARACTERÍSTICA MISC.<222> (590)..(590)<223> N É Α, C, G, OU T<400> 65

TTACTATAGG GCACGCGTGG TCGACGGCCCATTACATAAT CCAGCTTATA TCATAATCTATCTGTGTTGT TGTTTACCTA CTAACTTATTAATAAACGGG TTCTAAAATG GTCTAGGGTCGTAGTGTTAA GCTAAATCGA CATTTCTTTGGTTAAGCTAA AGCGACATTT CTTTGTGGGTTAGTGTTAAG CTAAATGTCG TTCTTTGTGGAAGCTAAATG TCGTTCTTTG TGGGTTGTGGTTAAGCTAAA TCGACATTTC TTTGTGGGTTAGTTGTGCAA GGTGTTCCTT ATAGCATCTCAATATAGGAC TCTAACCAAC AAAAACATACATCAAATGAT TATAGGGTCG ATTCTTCGGGTCTATCCTCA TTTTATATAT TATTTCTAAATTTATTTCCT AATACTATGA TATAGGGCTCATAAATACTT TAAATTAGGT CCTATTTTAATGTTTGTGCA TGATTGCTAT TTAGGTAGTTATTCTAGTAT TTTTTTTTGG TTCTCCGCTCAGCAGACTAT TTGATCTATT AAATTATGATTTTCACCATC AGTCCAAGTA TCTTTATAAAGTTAGATTAT AATTTAACGT ATCAGATGGTCTTGCATAAC AGTCGTTCTC TTTGTTATATACAAATGGTA CATACAAGTA GTGAACACATGACATCCTAT TTTTTAGAAC AAATTTCATACTAATTTTTT TATAGTATCA AGCATGTTATTTTATCCATT ATTTGCTCCC TACAATTAAAACCGAACCCG TATCCGTTTC ATATTCAAATAAAAATTTGA GGTTTAGTTT TTACAAATCTTGGATTTACA TGGTAAATTC TATGTCTTATATCTGACATT CGAATAAACA TTTGTTTCCATCCACTTCGT AATACGATAC AAAATCACCCCACTCAGTAA ACAATATTGT CTATGGTACAGATCTGCTCG ATTCAGGCAT CCTTGACACAAATAGAGCAG ACCTAATGGA TGGACCGTGGTCCGCCCGAT TGGTCATGGG GTCTGGGTTGCCACAGGTGC GCCACAACAG GATAGCCCAAATTATAGTTA GTATAAAGTA AAAAAACAAATTAAATAACT CTTTAAAGCT AGCAACTATGΤΤΤΤΤΤΤΑΤΤ TAAACAATAT GAGCCTTATAGACACATTAT AATTACTGAT CTAGCAGGCCGCGGACCAAG AGCTGGTCCG TTGGCTAATCCTGGGCCTAG CCGTGTCTGT GACTGGGCATGTACAAGAGG GGAATTTATA AATGAGGAGGGTTTTGTTGT GCTCAGCGAT AGATTTCAACGGGGGAAAAG GCCACATCAA AGGCGAGGTGTAAGTCCAGC AGCTAGCAAT GAAAGGGTACCATCCCCTCA ATGATCCGGT GCTCTCTTTT

TGGCTGGTCC TTGTTTGATT TACTTCCAGG 60GGTATCTAGA TTACATAATC TATCTAATAA 120TATAAGCTGG GTTATATAAT CTTGAGGCCA 180CAGTGTTAAG CTAAATCGAC ATTATGTCTA 24 0TGGGATGGGT CCGATGTGTC GTCTAGTAGT 300TGTGGAAGGT GTCCCTGCTC TCTAAGTTGT 360GTTGTGGCTG CTCCCTAAGT TGTTAGTGTT 4 20AAGGTGTTCC TTTTCCTTAA ATTGTTAGTG 4 80GTGGAANGTG TTCCTTTTCC TTAAGTTGTT 54 0CCACATGAGC CATAATGGAN TTTATTTTGA 600TCCAATAGGG ATTCTATTTT ACAAAAAAAT 660TCCTAAATAT AGTATCTAAT ATAATGGAGC 720ΤΤΤΤΤΑΤΤΤΑ CTAAATAACA TGTAACATGA 7 80AACTGTTGGA GCTGCAAACG TTTTTTGGCA 84 0TTTGAAAGAC TATATCATGC TCTTAGCGAG 900CAGTTGGGGG CTTTGATATG TTTAGTTGGA 960TTTTGACTAT CACAACGATC GCTATGCGCG 1020CCAACCATGT CACATTAAGC ACTTAAACTC 1080AAACCCTAAC AAACCACAAT TGCATATGTG 1140TCGCTTGCAC TCTTACACAC CTAGAAACTG 1200AATGCTTTAG TAATCATGAG CTAAGGGTAA 12 60CCTCGCTACC TATCTATAGG GGTGGAACTA 1320TTTTAAAATA GATATGCTTG AAAATTTATG 1380TACACATAAG AATAAAATTT TGTATAAATT 144 0AAGGTGAGAA AGCAAAAAGG TGAAGAAACA 1500TTTTACATCT ATTATTTGAG AATATATTTG 1560TTACAAGGTT AATGTTAAAT TATAAGACTG 1620TTGTCTGCGA TCGAAGAAAA ATGACAAAAA 1680CTCCTACCTA TCTCACCTCC TATTTCAAAC 17 40CCTATCTATC TCACCTCCTA TTTCAAACTC 1800AAATCAAGTG TTTTGTACAT CTATTTGCAC 18 60CAACATACTC CTTAGGGCTA TAAATGTCCA 1920CATGACACGA CTTATCCCAA CACAGCACAG 1980GTCTAGCCTG ATCATCGGGT CACTCTTGGG 204 0CCTATCCTAT TTTTTCATGC ATATATCTAT 2100AAGTATGTGT GTTATGTTGG CTAGATGTGT 2160GTTTAAATCA TACATATACA CATTTTTAGT 2220GGCACGTCGA GTGTGACGGG CCAGTGAGAT 2280GTATCTAGGT CTTTCTCGCG GACCTTTCTC 234 0TATACGGTAC CGATACTGTC CTAATTCATA 2400GGCTAGCGAA GCCCGCCCAT TTGAACACCT 24 60AATGTACTCA TGCGGTACAC CAGGGGAATT 2520GCAACGGTGA GCCAGTTTCA CCAAAAAAAA 2580CAGACGAGCA GAAGATGCTA GCAGTGCAGC 2 64 0TCAGGATTTA ACAATGCCTA GAGACGGCAT 2700TGTTTATTCA CCCGTTGGCG TAACTATΑΤΑ 27 60CACATGTCTG GTCTGACGAA CGAATCAAGGCTGTCATGAC ATGTGCTCTG CCTCTGCTGGTTTCTTGTGC TAGAGGCGAG TCAAACAAAC

GATCGCTAGC TCGGGCGAGC CTTCCTATCA 2820TTGATAAGCC GTGCGCCTTC TCGCTAATTC 2880GCTGCAC 2917

<210> 66

<211> 4498

<212> DNA

<213> Zea mays

<220>

<221> CARACTERÍSTICA MISC.

<222> (517)..(517)

<223> N É Α, C, G, OU T

<220>

<221> CARACTERÍSTICA MISC.

<222> (590)..(590)

<223> N É Α, C, G, OU T

<400> 66

TTACTATAGG GCACGCGTGG TCGACGGCCCATTACATAAT CCAGCTTATA TCATAATCTATCTGTGTTGT TGTTTACCTA CTAACTTATTAATAAACGGG TTCTAAAATG GTCTAGGGTCGTAGTGTTAA GCTAAATCGA CATTTCTTTGGTTAAGCTAA AGCGACATTT CTTTGTGGGTTAGTGTTAAG CTAAATGTCG TTCTTTGTGGAAGCTAAATG TCGTTCTTTG TGGGTTGTGGTTAAGCTAAA TCGACATTTC TTTGTGGGTTAGTTGTGCAA GGTGTTCCTT ATAGCATCTCAATATAGGAC TCTAACCAAC AAAAACATACATCAAATGAT TATAGGGTCG ATTCTTCGGGTCTATCCTCA TTTΤΑΤΑΤΑΤ TATTTCTAAATTTATTTCCT AATACTATGA TATAGGGCTCATAAATACTT TAAATTAGGT CCTATTTTAATGTTTGTGCA TGATTGCTAT TTAGGTAGTTATTCTAGTAT TTTTTTTTGG TTCTCCGCTCAGCAGACTAT TTGATCTATT AAATTATGATTTTCACCATC AGTCCAAGTA TCTTTATAAAGTTAGATTAT AATTTAACGT ATCAGATGGTCTTGCATAAC AGTCGTTCTC TTTGTTATATACAAATGGTA CATACAAGTA GTGAACACATGACATCCTAT TTTTTAGAAC AAATTTCATACTAATTTTTT TATAGTATCA AGCATGTTATTTTATCCATT ATTTGCTCCC TACAATTAAAACCGAACCCG TATCCGTTTC ATATTCAAATAAAAATTTGA GGTTTAGTTT TTACAAATCTTGGATTTACA TGGTAAATTC TATGTCTTATATCTGACATT CGAATAAACA TTTGTTTCCATCCACTTCGT AATACGATAC AAAATCACCCCACTCAGTAA ACAATATTGT CTATGGTACAGATCTGCTCG ATTCAGGCAT CCTTGACACAAATAGAGCAG ACCTAATGGA TGGACCGTGGTCCGCCCGAT TGGTCATGGG GTCTGGGTTGCCACAGGTGC GCCACAACAG GATAGCCCAAATTATAGTTA GTATAAAGTA AAAAAACAAATTAAATAACT CTTTAAAGCT AGCAACTATGΤΤΤΤΤΤΤΑΤΤ TAAACAATAT GAGCCTTATAGACACATTAT AATTACTGAT CTAGCAGGCC

TGGCTGGTCC TTGTTTGATT TACTTCCAGG 60GGTATCTAGA TTACATAATC TATCTAATAA 120TATAAGCTGG GTTATATAAT CTTGAGGCCA 180CAGTGTTAAG CTAAATCGAC ATTATGTCTA 240TGGGATGGGT CCGATGTGTC GTCTAGTAGT 300TGTGGAAGGT GTCCCTGCTC TCTAAGTTGT 360GTTGTGGCTG CTCCCTAAGT TGTTAGTGTT 420AAGGTGTTCC TTTTCCTTAA ATTGTTAGTG 4 80GTGGAANGTG TTCCTTTTCC TTAAGTTGTT 54 0CCACATGAGC CATAATGGAN TTTATTTTGA 600TCCAATAGGG ATTCTATTTT ACAAAAAAAT 660TCCTAAATAT AGTATCTAAT ATAATGGAGC 720ΤΤΤΤΤΑΤΤΤΑ CTAAATAACA TGTAACATGA 780AACTGTTGGA GCTGCAAACG TTTTTTGGCA 84 0TTTGAAAGAC TATATCATGC TCTTAGCGAG 900CAGTTGGGGG CTTTGATATG TTTAGTTGGA 960TTTTGACTAT CACAACGATC GCTATGCGCG 1020CCAACCATGT CACATTAAGC ACTTAAACTC 1080AAACCCTAAC AAACCACAAT TGCATATGTG 114 0TCGCTTGCAC TCTTACACAC CTAGAAACTG 1200AATGCTTTAG TAATCATGAG CTAAGGGTAA 1260CCTCGCTACC TATCTATAGG GGTGGAACTA 1320TTTTAAAATA GATATGCTTG AAAATTTATG 1380TACACATAAG AATAAAATTT TGTATAAATT 14 4 0AAGGTGAGAA AGCAAAAAGG TGAAGAAACA 1500TTTTACATCT ATTATTTGAG AATATATTTG 1560TTACAAGGTT AATGTTAAAT TATAAGACTG 1620TTGTCTGCGA TCGAAGAAAA ATGACAAAAA 1680CTCCTACCTA TCTCACCTCC TATTTCAAAC 17 40CCTATCTATC TCACCTCCTA TTTCAAACTC 1800AAATCAAGTG TTTTGTACAT CTATTTGCAC 18 60CAACATACTC CTTAGGGCTA TAAATGTCCA 1920CATGACACGA CTTATCCCAA CACAGCACAG 1980GTCTAGCCTG ATCATCGGGT CACTCTTGGG 2040CCTATCCTAT TTTTTCATGC ATATATCTAT 2100AAGTATGTGT GTTATGTTGG CTAGATGTGT 2160GTTTAAATCA TACATATACA CATTTTTAGT 2220GGCACGTCGA GTGTGACGGG CCAGTGAGAT 2280GTATCTAGGT CTTTCTCGCG GACCTTTCTC 234 0GCGGACCAAG AGCTGGTCCG TTGGCTAATCCTGGGCCTAG CCGTGTCTGT GACTGGGCATGTACAAGAGG GGAATTTATA AATGAGGAGGGTTTTGTTGT GCTCAGCGAT AGATTTCAACGGGGGAAAAG GCCACATCAA AGGCGAGGTGTAAGTCCAGC AGCTAGCAAT GAAAGGGTACCATCCCCTCA ATGATCCGGT GCTCTCTTTTCACATGTCTG GTCTGACGAA CGAATCAAGGCTGTCATGAC ATGTGCTCTG CCTCTGCTGGTTTCTTGTGC TAGAGGCGAG TCAAACAAACAGGGTCACAT GACCCTGTTC CCTATCGCTAACTTACGACG CGTCCGTACG CGGCAGGCCTGGCATTCGCC CCCTGCCAGC CGGTTCGCAGGCATCCCAAA CGCCCGGGAA CCTTTGGGGCTGGCTTCGTA AAGCTCTGAC TTTTGATCACGCCGACACGG CCGCAAAGTC AACTACCCGCTATATATGTT CGCGGCCATG TCCGTACTAGTCAGATCCCT CGCCTCGTGT CGTGTCTCCGAGACGGACAC CGCCGAGCTA TAGCGCTTGGGGAGCACGTA CGTACGTACC GCAGCGATGGTGCTGGCGGC GCTTCTCTTC GCCGCCTCCCTCTCCTCGCT GCCCAAAGCG CTCGACGTCAGCGCGTTCCG GCCCGGCTCA TAGTCATAGCGTTAATTCAT AGTCCTATTC TTCTCTATGTCAATCAAGGA GCTAGCTGAT TAAAATACACGTCCTGCACG CCGGCGTGGA CTCGCTGACGGCCGGCGCCG ACGCCGGGTA CAAGGGCGTGCAGAAGGACC GCGGGTGGCG CAAGTCCGAGTTCAAGGTCA CCGAGCAGGC GTACGCGGCGGCCCGCGACG TCCCCTCGGG CTCCTACTACGCCGAGGTGG CCTACGGCCA GACGGCGCCCGGCATCCACG CCTCTCTCAA GATCGCCGCCCTCGCCTTCT TCTTCGTCAT CGAGACCCGCCGCGCCATAC ATGCATACAT GTAAATCGTCCATTGGTGCG CGCGACTATT TTGGTGTATCCAAAATTACC AAATAATAAC TTAAGTTTCT

TATACGGTAC CGATACTGTC CTAATTCATA 24 00GGCTAGCGAA GCCCGCCCAT TTGAACACCT 24 60AATGTACTCA TGCGGTACAC CAGGGGAATT 2520GCAACGGTGA GCCAGTTTCA CCAAAAAAAA 2580CAGACGAGCA GAAGATGCTA GCAGTGCAGC 2640TCAGGATTTA ACAATGCCTA GAGACGGCAT 27 00TGTTTATTCA CCCGTTGGCG TAACTATATA 27 60GATCGCTAGC TCGGGCGAGC CTTCCTATCA 2820TTGATAAGCC GTGCGCCTTC TCGCTAATTC 2880GCTGCACCTC GTAGCCCTTA ATCTGCGCTA 294 0GTTACCAACG ACCCATTCCC CCTGACAGAT 3000CGGCAGTTCG GCATCACCAG CACCGGCGCC 3060ATTCGCAGGG CGGAGTCGGC CGCAGTTGCC 3120CCCTCTACGA GCAAATGAAG TTGCTGCCCC 3180TTGATTGGCA GTCGTACTCC TCGCTCATAG 324 0TCCGCCATCC TTCAACCCCC GCCACGCGCC 3300TCCTCCAACC CACAAGCCAC AACCCCGAGC 3360GTCGACGACG ACCAACAGCC AGTGTGGGCC 34 20TGATAGCAAG GGACGACCGG CGGCCGGACC 34 80CTCGGCAGCA AAGCGTGCAG GCCTTGTGTG 3540TGCCGTCGCC GGCCGCCGCG GGGGTGCACC 3600CCACCTCCGC CAAACCCGGC CAAGGTGCGC 3660CAAAGGATTA GCACTTTGAT TACTTGCTCG 3720TTGAAACCCC CCTTTAGATT TGTTCATTCA 3780ACGATTGCCA TAAAATATAT GCTTCTCGCA 3840GTGACGTGGA GCCTGAACGC CACGGAGCCG 3900AAGGTGAAGC TGTGCTACGC GCCGGCGAGC 3960GACGACATCA GCAAGGACAA GGCGTGCCAG 4 020GCGGCGCCCG GCAGCTTCCA GTACGCCGTC 4 080CTGCGCGCCT TCGCCACGGA CGCGTCGGGC 4140ACCGCCGCCT TCGACGTCGC CGGCATCACC 4 200GGCGTCTTCT CGGCCTTCTC CGTCGTCGCG 4 2 60AAGAAGAACA AGTAGAACGA GTTGCGGCTG 4 320GGCGGCGATG AGTGGCTGTC GTTGCTGATT 4 380ATGTAAGTTA CTTTTCTGCA GTGTGTGCGT 4440CTGCTAAAAA AAAAAAAAAA AAAAAAAA 4 4 98

<210> 67

<211> 3506

<212> DNA

<213> Zea mays

<220>

<221> CARACTERÍSTICA MISC.

<222> (517)..(517)

<223> N É A, C, G, OU T

<220>

<221> CARACTERÍSTICA MISC.

<222> (590)..(590)

<223> N É A, C, G, OU T

<4 00> 67

TTACTATAGG GCACGCGTGG TCGACGGCCCAT TACATAAT C CAG CTTATA T CATAAT CTATCTGTGTTGT TGTTTACCTA CTAACTTATTAATAAACGGG TTCTAAAATG GTCTAGGGTCGTAGTGTTAA GCTAAATCGA CATTTCTTTGGTTAAGCTAA AGCGACATTT CTTTGTGGGT

TGGCTGGTCC TTGTTTGATT TACTTCCAGG 60

GGTATCTAGA TTACATAATC TATCTAATAA 120

TATAAGCTGG GTTATATAAT CTTGAGGCCA 180

CAGTGTTAAG CTAAATCGAC ATTATGTCTA 240

TGGGATGGGT CCGATGTGTC GTCTAGTAGT 300

TGTGGAAGGT GTCCCTGCTC TCTAAGTTGT 360tagtgttaag ctaaatgtcg ttctttgtggaagctaaatg tcgttctttg tgggttgtggttaagctaaa tcgacatttc tttgtgggttagttgtgcaa ggtgttcctt atagcatctcaatataggac tctaaccaac aaaaacatacatcaaatgat tatagggtcg attcttcgggtctatcctca ttttatatat tatttctaaatttatttcct aatactatga tatagggctcataaatactt taaattaggt cctattttaatgtttgtgca tgattgctat ttaggtagttattctagtat ttttttttgg ttctccgctcagcagactat ttgatctatt aaattatgattttcaccatc agtccaagta tctttataaagttagattat aatttaacgt atcagatggtcttgcataac agtcgttctc tttgttatatacaaatggta catacaagta gtgaacacatgacatcctat tttttagaac aaatttcatactaatttttt tatagtatca agcatgttattttatccatt atttgctccc tacaattaaaaccgaacccg tatccgtttc atattcaaataaaaatttga ggtttagttt ttacaaatcttggatttaca tggtaaattc tatgtcttatatctgacatt cgaataaaca tttgtttccatccacttcgt aatacgatac aaaatcaccccactcagtaa acaatattgt ctatggtacagatctgctcg attcaggcat ccttgacacaaatagagcag acctaatgga tggaccgtggtccgcccgat tggtcatggg gtctgggttgccacaggtgc gccacaacag gatagcccaaattatagtta gtataaagta aaaaaacaaattaaataact ctttaaagct agcaactatgtttttttatt taaacaatat gagccttata

gacacattat aattactgat ctagcaggccgcggaccaag agctggtccg ttggctaatcctgggcctag ccgtgtctgt gactgggcatgtacaagagg ggaatttata aatgaggagggttttgttgt gctcagcgat agatttcaacgggggaaaag gccacatcaa aggcgaggtgtaagtccagc agctagcaat gaaagggtaccatcccctca atgatccggt gctctcttttcacatgtctg gtctgacgaa cgaatcaaggctgtcatgac atgtgctctg cctctgctggtttcttgtgc tagaggcgag tcaaacaaacagggtcacat gaccctgttc cctatcgctaacttacgacg cgtccgtacg cggcaggcctggcattcgcc ccctgccagc cggttcgcaggcatcccaaa cgcccgggaa cctttggggctggcttcgta aagctctgac ttttgatcacgccgacacgg ccgcaaagtc aactacccgctatatatgtt cgcggccatg tccgtactagtcagatccct cgcctcgtgt cgtgtctccgagacggacac cgccgagcta tagcgcttggggagcacgta cgtacgtacc gcagcg

gttgtggctg ctccctaagt tgttagtgtt 420aaggtgttcc ttttccttaa attgttagtg 480gtggaangtg ttccttttcc ttaagttgtt 540ccacatgagc cataatggan tttattttga 600tccaataggg attctatttt acaaaaaaat 660tcctaaatat agtatctaat ataatggagc 720tttttattta ctaaataaca tgtaacatga 780aactgttgga gctgcaaacg ttttttggca 840tttgaaagac tatatcatgc tcttagcgag 900cagttggggg ctttgatatg tttagttgga 960ttttgactat cacaacgatc gctatgcgcg 1020ccaaccatgt cacattaagc acttaaactc 1080aaaccctaac aaaccacaat tgcatatgtg 1140tcgcttgcac tcttacacac ctagaaactg 1200aatgctttag taatcatgag ctaagggtaa 1260cctcgctacc tatctatagg ggtggaacta 1320ttttaaaata gatatgcttg aaaatttatg 1380tacacataag aataaaattt tgtataaatt 1440aaggtgagaa agcaaaaagg tgaagaaaca 1500ttttacatct attatttgag aatatatttg 1560ttacaaggtt aatgttaaat tataagactg 1620ttgtctgcga tcgaagaaaa atgacaaaaa 1680ctcctaccta tctcacctcc tatttcaaac 1740cctatctatc tcacctccta tttcaaactc 1800aaatcaagtg ttttgtacat ctatttgcac 1860caacatactc cttagggcta taaatgtcca 1920catgacacga cttatcccaa cacagcacag 1980gtctagcctg atcatcgggt cactcttggg 2040cctatcctat tttttcatgc atatatctat 2100aagtatgtgt gttatgttgg ctagatgtgt 2160gtttaaatca tacatataca catttttagt 2220ggcacgtcga gtgtgacggg ccagtgagat 2280gtatctaggt ctttctcgcg gacctttctc 2340tatacggtac cgatactgtc ctaattcata 2400ggctagcgaa gcccgcccat ttgaacacct 2460aatgtactca tgcggtacac caggggaatt 2520gcaacggtga gccagtttca ccaaaaaaaa 2580cagacgagca gaagatgcta gcagtgcagc 2640tcaggattta acaatgccta gagacggcat 2700tgtttattca cccgttggcg taactatata 2760gatcgctagc tcgggcgagc cttcctatca 2820ttgataagcc gtgcgccttc tcgctaattc 2880gctgcacctc gtagccctta atctgcgcta 2940gttaccaacg acccattccc cctgacagat 3000cggcagttcg gcatcaccag caccggcgcc 3060attcgcaggg cggagtcggc cgcagttgcc 3120ccctctacga gcaaatgaag ttgctgcccc 3180ttgattggca gtcgtactcc tcgctcatag 3240tccgccatcc ttcaaccccc gccacgcgcc 3300tcctccaacc cacaagccac aaccccgagc 3360gtcgacgacg accaacagcc agtgtgggcc 3420tgatagcaag ggacgaccgg cggccggacc 3480

3506

<210> 68

<211> 1014

<212> dna

<213> Zea mays<400> 68

CACAACGATC GCTATGCGCG AGCAGACTATCACATTAAGC ACTTAAACTC TTTCACCATCAAACCACAAT TGCATATGTG GTTAGATTATTCTTACACAC CTAGAAACTG CTTGCATAACTAATCATGAG CTAAGGGTAA ACAAATGGTATATCTATAGG GGTGGAACTA GACATCCTATGATATGCTTG AAAATTTATG CTAATTTTTTAATAAAATTT TGTATAAATT TTTATCCATTAGCAAAAAGG TGAAGAAACA ACCGAACCCGATTATTTGAG AATATATTTG AAAAATTTGAAATGTTAAAT TATAAGACTG TGGATTTACATCGAAGAAAA ATGACAAAAA ATCTGACATTTCTCACCTCC TATTTCAAAC TCCACTTCGTTCACCTCCTA TTTCAAACTC CACTCAGTAATTTTGTACAT CTATTTGCAC GATCTGCTCGCTTAGGGCTA TAAATGTCCA AATAGAGCAGCTTATCCCAA CACAGCACAG TCCGCCCGAT

TTGATCTATT AAATTATGAT CCAACCATGT 60

AGTCCAAGTA TCTTTATAAA AAACCCTAAC 120

AATTTAACGT ATCAGATGGT TCGCTTGCAC 180

AGTCGTTCTC TTTGTTATAT AATGCTTTAG 240

CATACAAGTA GTGAACACAT CCTCGCTACC 300TTTTTAGAAC AAATTTCATA ΤΤΤΤΑΑΑΑΤΑ 360

TATAGTATCA AGCATGTTAT TACACATAAG 4 20

ATTTGCTCCC TACAATTAAA AAGGTGAGAA 4 80

TATCCGTTTC ATATTCAAAT TTTTACATCT 540

GGTTTAGTTT TTACAAATCT TTACAAGGTT 600

TGGTAAATTC TATGTCTTAT TTGTCTGCGA 660

CGAATAAACA TTTGTTTCCA CTCCTACCTA 720

AATACGATAC AAAATCACCC CCTATCTATC 780

ACAATATTGT CTATGGTACA AAATCAAGTG 84 0

ATTCAGGCAT CCTTGACACA CAACATACTC 900

ACCTAATGGA TGGACCGTGG CATGACACGA 960

TGGTCATGGG GTCTGGGTTG GTCT 1014

<210> 69

<211> 1492

<212> DNA

<213> Zea mays

<4 00> 69

AGCCTGATCA TCGGGTCACT CTTGGGCCACTCCTATTTTT TCATGCATAT ATCTATATTAATGTGTGTTA TGTTGGCTAG ATGTGTTTAAAAATCATACA TATACACATT TTTAGTTTTTCGTCGAGTGT GACGGGCCAG TGAGATGACACTAGGTCTTT CTCGCGGACC TTTCTCGCGGCGGTACCGAT ACTGTCCTAA TTCATACTGGAGCGAAGCCC GCCCATTTGA ACACCTGTACTACTCATGCG GTACACCAGG GGAATTGTTTCGGTGAGCCA GTTTCACCAA AAAAAAGGGGCGAGCAGAAG ATGCTAGCAG TGCAGCTAAGGATTTAACAA TGCCTAGAGA CGGCATCATCTATTCACCCG TTGGCGTAAC TATATACACAGCTAGCTCGG GCGAGCCTTC CTATCACTGTTAAGCCGTGC GCCTTCTCGC TAATTCTTTCCACCTCGTAG CCCTTAATCT GCGCTAAGGGCCAACGACCC ATTCCCCCTG ACAGATACTTAGTTCGGCAT CACCAGCACC GGCGCCGGCAGCAGGGCGGA GTCGGCCGCA GTTGCCGCATCTACGAGCAA ATGAAGTTGC TGCCCCTGGCTTGGCAGTCG TACTCCTCGC TCATAGGCCGCCATCCTTCA ACCCCCGCCA CGCGCCTATACCAACCCACA AGCCACAACC CCGAGCTCAGACGACGACCA ACAGCCAGTG TGGGCCAGACAGCAAGGGAC GACCGGCGGC CGGACCGGAG

AGGTGCGCCA CAACAGGATA GCCCAACCTA 60TAGTTAGTAT AAAGTAAAAA AACAAAAAGT 120ATAACTCTTT AAAGCTAGCA ACTATGGTTT 180TTTATTTAAA CAATATGAGC CTTATAGGCA 24 0CATTATAATT ACTGATCTAG CAGGCCGTAT 300ACCAAGAGCT GGTCCGTTGG CTAATCTATA 360GCCTAGCCGT GTCTGTGACT GGGCATGGCT 4 20AAGAGGGGAA TTTATAAATG AGGAGGAATG 4 80TGTTGTGCTC AGCGATAGAT TTCAACGCAA 54 0GAAAAGGCCA CATCAAAGGC GAGGTGCAGA 600TCCAGCAGCT AGCAATGAAA GGGTACTCAG 660CCCTCAATGA TCCGGTGCTC TCTTTTTGTT 720TGTCTGGTCT GACGAACGAA TCAAGGGATC 780CATGACATGT GCTCTGCCTC TGCTGGTTGA 84 0TTGTGCTAGA GGCGAGTCAA ACAAACGCTG 900TCACATGACC CTGTTCCCTA TCGCTAGTTA 960ACGACGCGTC CGTACGCGGC AGGCCTCGGC 1020TTCGCCCCCT GCCAGCCGGT TCGCAGATTC 1080CCCAAACGCC CGGGAACCTT TGGGGCCCCT 114 0TTCGTAAAGC TCTGACTTTT GATCACTTGA 1200ACACGGCCGC AAAGTCAACT ACCCGCTCCG 1260TATGTTCGCG GCCATGTCCG TACTAGTCCT 1320ATCCCTCGCC TCGTGTCGTG TCTCCGGTCG 1380GGACACCGCC GAGCTATAGC GCTTGGTGAT 14 4 0CACGTACGTA CGTACCGCAG CG 14 92

<210> 70

<211> 3621

<212> DNA

<213> Zea mays

<400> 70

TGGTCCTTGT TTGATTTACT TCCAGGATTA TATAATCCAG CTTATGGATT ATATAAGTAC 60CTATTGACGT CACGTGCTTA TGTATTATAA TAATCTAGGT ATATAGATTA TATAATCTAT 120CTAATAATAA TCTGTGTTGT TTGTTTATCT CTCAAAACAA ACAGGTCCTA AAATGGTCCC 180GGGCGTCCAA TGTGTCGTCA AGTAGTGTTA AGCTAAATCG ACATTTCTTT GTGGGTTGTG 24 0TGGAAGGTGT TCCTTTTCCT TAAGTTGTTA GTTGTGCAAG GTGTTCCTTA GAGCATCTCC 300AATAGGACCT ATAATGGATT CTATTTTGAA TTATAAGACT CTAACAACAA AAGCATACTT 360TAATGGGGAT TCTATTTTAC ΑΑΑΑΑΑΑΤΑΤ CAAATGATTA TATGGTCGAT TCCTCGGGTC 4 20CTAAATATAG TATCTCATAT AATAGAGCTC TATCCTCATT TTATATACTA TTTTTAAGTT 4 80TTTATTTACT AAATAACATG ATTTATTTTC TAATACTATG AACTCAACTA TTAGAGCTGT 54 0AAACGTTTTT GTGGTACTAA ACACTTTAAA TCAGGTCCTA TTTTAATTTG AAGGACTTAA 600ATATAAGACT TCTGGTTAGA GATGCTCTTA GCGAGTGTTT GTGCATGATT GCTATTTAGT 660CTTTGTGGAT TGTGGAAGGT GTTACTTTTC CTCAAGTTGT TAGTTGTGCA AGGTGTTTCT 720TAGAGCATCT CTAACAGGAG CCTTAACGGA ATCTATTTTG AAGTATAGTA CTTTAACACC 780AAAAACATAC TTTAATAGGG GTCCTATTTT ACAAAAAAAT TATCAAATGA TTATAAGGTC 84 0CACTCCTCGG GTCCTAAATA TAATATCTCA TATACTAGAG CTCTATCCTC ΑΤΤΤΤΑΤΑΤΑ 900CTATCCCTAG GTTTTTATTC CCTAAATAAC ATGATTTATT TCCTAATACT AAGATATAGG 960GCTCAACTAT TGGAGTTGCA AATGTTTTTT GGCACTAAAC ACTTTATATC AGGTCCTATT 1020ΤΤΑΑΤΤΤΤΑΑ TTTGAAGGAC TCAAATATAG GACTTCTCGT TAGAGATGCT CTTAGCGAGT 1080GTTTGTGCAT GATTGCTATT TATGTCTGTA GTTTAGTTGG GGGCTTTAAT ATGTTTAGTT 114 0GAAGTTCTAG TATTTTTTAG GTTCTCCACT CTTTGGATTA TGACAACGAC CACTATCCAA 1200GCAGTCTTTG AGTGCAAACG CGCGAGCAAA CTATCTGATC ΤΑΤΤΑΑΑΤΤΑ TGATCCAACC 12 60GTTATGTCAT ATTGAAGACT TAAACCCTTT CACCACCAGC CCAAGTATCT TTATGAAAAA 1320CCCTAACAAA CCACAATTGC ATCTATGGTT GGATTATAAT TTAACGTATC AGATGGTTCG 1380CTTGCATGCT TACATATCTA GAAACTGTTT GCATAACAGT CGTTCTCTTT GGTTATATAA 14 4 0TGCTTTAGTA ATCATCAGCC AAGTGTAAAC AAATGGTACA AACTAGTAGT GAACACATCC 1500TCCCTACCTA TCTCTAGGGG TGTAACTAGA TATCCGAATT CTTAGAACAA ATTTCATATT 1560TTAAAATAGA TATGCTTCAA AATTTATGCT AATCTTTTTT ATATTATCAA GCATATTATT 1620ACACATAAGA ΑΤΑΑΑΑΤΤΤΤ GTATAGAATT TTATCCATTA TTTGTTCCCT AGAATTTAAA 1680AAGTGAAAAA ACATTCGAAT CTGTATCAGT TTCGTATTCA AATTTTTACA TCTATTATTT 17 4 0GAGAATATAT ATGATAAATT TGAGGTTTAG TTTTTATGAA TCTTTACAAG GTTAATGTTA 1800AATACATGAC TATGGATTTA CATAGTAAAT TCTATGTCTT ATTTGTCCGC GATTGAAGAA 18 60AAATGACAAA AAGATCTGAC ATTCGAATAA ACATCTGTTT CCACTCCTAC CTATCTGACC 1920TCCTATTTCA AACTCCACTT TGTAACACGG TACAAAATCA CTCCCTACCT ATCTGACCTC 1980CTATTTCAAA CTCCACTCAG TAAACAATAT TGTCTATGGT ACAAAACCAA GTGTTTTATA 204 0CATCTATTTG CACGATCTGC TCGAGTCAGG CATCCTTGAC ACACAACATA CTCCTTGTGG 2100CTATAAATGT CCAAATAGAG CAGACCTAAT GGGTGGACCG TTGCATGACA CGACTTATCC 2160CAAGACGAGC ACAGTTCGCC CCATTGGTCA TGGGGGTCCG GGCTAGTCTA GCCTGATCAT 2220CGGGTCACAC TTAGGCCACA GGTGTGCCAC AACGGGATAG CCCAACATGT CCCTTTTTGT 22 80CATGCATATA TCTATATTAT AGTTAGTATA ATGTAAAAAA ACAAAAGGTA TGTGTGTTAT 234 0GTTGGTTAGA TGTGTTTAAA TAACTCTTTA AAGCTAGCAA CTATGGTTTA AATCATACAT 24 00ATACACATTT ΤΤΑΤΤΤΤΑΤΤ ΤΤΤΑΤΤΤΑΑΑ CGATATGGGC CTTCTAGGCA CGTCGAGTGT 24 60GACGGGCCAG TGAGATGACA CATTATAATT ACTGGTCTAG CAGGCCGTAC CTAGGTCTTT 2520CTCGTGGGCC AAGACTAAGG GTTGGCCCGT TGGCTAATCT GTACGGTACC GATACTGTCC 2580TAATTCATTT GAACACCTGT AGAAGAGGGG ΑΑΤΤΤΑΤΑΑΤ TGAGGAGGAA TGTACTCATG 2640CGGTACACCA GGGGAATTGT TTTGTTGTGC TCAGCGATAG ATTTCAACGC AACGGTGAGC 27 00CAGTTTCACT AAAAAAAGGG GGGGGGGGGG GGGGGGGGGA AGGCCACATC AAAGGCGAGG 27 60TGCTGACGAG CAGAAGATGC TAGCAGTGAC GCCAAGTCCA GCAGCTAGCA ATGAAAGGGT 2820ACTCGGGATT TAACAATGCC TAGAGACGGC ATCATCCCCT CAATAATCCG GTGCTCTCTT 2880TTTGTTTATT CACCAGTTGG CGTAGCTATA TACACATGTC TGGTCTGACG AACAAATCAA 2940GGGATCGCTA GCTCGGGCTA GCCTTCCTAT CACTGTCATG ACATGTGCTC TGCCTCTGCT 3000GGTTGATAAG CCGTGCGCCT TCTCGCTAAT TCTTTCTTGT GCTAGAGGCG AGTCAAACAA 3060ACGCTGCACC TCGTAGCCCT TAATCTGCGC TAAGGGTCAC ATGACCCTGT TCCCTATCGC 3120TAGTTACCAA CGACCCATTC CCCCTGACAG ATACTTACGA CGCGTCCGTA CGCGGCAGGC 3180CTCGGCAGTT CGGCATCACC AGCACCGGCG CCGGCATTCG CCCCCTGCCA GCCGGTTCGC 324 0AGATTCGCAG GGCGGAGTCG GCCGCAGTTG CCGCATCCCA AACGCCCGGG AACCTTTGGG 3300GCCCCTCTAC GAGCAAATGA AGTTGCTGCC CCTGGCTTCG TAAAGCTCTG ACTTTTGATC 3360ACTTGATTGG CAGTCGTACT CCTCGCTCAT AGGCCGACAC GGCCGCAAAG TCAACTACCC 3420GCTCCGCCAT CCTTCAACCC CCGCCACGCG CCTATATATG TTCGCGGCCA TGTCCGTACT 34 80AGTCCTCCAA CCCACAAGCC ACAACCCCGA GCTCAGATCC CTCGCCTCGT GTCGTGTCTC 354 0CGGTCGACGA CGACCAACAG CCAGTGTGGG CCAGACGGAC ACCGCCGAGC TATAGCGCTT 3600GGTGATAGCA AGGGACGACC G 3621<210> 71

<211> 3236

<212> DNA

<213> Zea mays

<400> 71

TGGTCCTTGT TTGATTTACT TCCAGGATTACTATTGACGT CACGTGCTTA TGTATTATAACTAATAATAA TCTGTGTTGT TTGTTTATCTGGGCGTCCAA TGTGTCGTCA AGTAGTGTTATGGAAGGTGT TCCTTTTCCT TAAGTTGTTAAATAGGACCT ATAATGGATT CTATTTTGAATAATGGGGAT TCTATTTTAC ΑΑΑΑΑΑΑΤΑΤCTAAATATAG TATCTCATAT AATAGAGCTCTTTATTTACT AAATAACATG ATTTATTTTCAAACGTTTTT GTGGTACTAA ACACTTTAAAATATAAGACT TCTGGTTAGA GATGCTCTTACTTTGTGGAT TGTGGAAGGT GTTACTTTTCTAGAGCATCT CTAACAGGAG CCTTAACGGAAAAAACATAC TTTAATAGGG GTCCTATTTTCACTCCTCGG GTCCTAAATA TAATATCTCACTATCCCTAG GTTTTTATTC CCTAAATAACGCTCAACTAT TGGAGTTGCA AATGTTTTTTΤΤΑΑΤΤΤΤΑΑ TTTGAAGGAC TCAAATATAGGTTTGTGCAT GATTGCTATT TATGTCTGTAGAAGTTCTAG TATTTTTTAG GTTCTCCACTGCAGTCTTTG AGTGCAAACG CGCGAGCAAAGTTATGTCAT ATTGAAGACT TAAACCCTTTCCCTAACAAA CCACAATTGC ATCTATGGTTCTTGCATGCT TACATATCTA GAAACTGTTTTGCTTTAGTA ATCATCAGCC AAGTGTAAACTCCCTACCTA TCTCTAGGGG TGTAACTAGATTAAAATAGA TATGCTTCAA AATTTATGCTACACATAAGA ΑΤΑΑΑΑΤΤΤΤ GTATAGAATTAAGTGAAAAA ACATTCGAAT CTGTATCAGTGAGAATATAT ATGATAAATT TGAGGTTTAGAATACATGAC TATGGATTTA CATAGTAAATAAAT GACAAA AAGATCTGAC ATTCGAATAATCCTATTTCA AACTCCACTT TGTAACACGGCTATTTCAAA CTCCACTCAG TAAACAATATCATCTATTTG CACGATCTGC TCGAGTCAGGCTATAAATGT CCAAATAGAG CAGACCTAATCAAGACGAGC ACAGTTCGCC CCATTGGTCACGGGTCACAC TTAGGCCACA GGTGTGCCACCATGCATATA TCTATATTAT AGTTAGTATAGTTGGTTAGA TGTGTTTAAA TAACTCTTTAATACACATTT TTATTTTATT ΤΤΤΑΤΤΤΑΑΑGACGGGCCAG TGAGATGACA CATTATAATTCTCGTGGGCC AAGACTAAGG GTTGGCCCGTTAATTCATTT GAACACCTGT AGAAGAGGGGCGGTACACCA GGGGAATTGT TTTGTTGTGCCAGTTTCACT AAAAAAAGGG GGGGGGGGGGTGCTGACGAG CAGAAGATGC TAGCAGTGACACTCGGGATT TAACAATGCC TAGAGACGGCTTTGTTTATT CACCAGTTGG CGTAGCTATAGGGATCGCTA GCTCGGGCTA GCCTTCCTATGGTTGATAAG CCGTGCGCCT TCTCGCTAATACGCTGCACC TCGTAGCCCT TAATCTGCGC

TATAATCCAG CTTATGGATT ATATAAGTAC 60TAATCTAGGT ATATAGATTA TATAATCTAT 120CTCAAAACAA ACAGGTCCTA AAATGGTCCC 180AGCTAAATCG ACATTTCTTT GTGGGTTGTG 240GTTGTGCAAG GTGTTCCTTA GAGCATCTCC 300TTATAAGACT CTAACAACAA AAGCATACTT 360CAAATGATTA TATGGTCGAT TCCTCGGGTC 4 20TATCCTCATT TTATATACTA TTTTTAAGTT 4 80TAATACTATG AACTCAACTA TTAGAGCTGT 54 0TCAGGTCCTA TTTTAATTTG AAGGACTTAA 600GCGAGTGTTT GTGCATGATT GCTATTTAGT 660CTCAAGTTGT TAGTTGTGCA AGGTGTTTCT 720ATCTATTTTG AAGTATAGTA CTTTAACACC 780ACAAAAAAAT TATCAAATGA TTATAAGGTC 84 0TATACTAGAG CTCTATCCTC ΑΤΤΤΤΑΤΑΤΑ 900ATGATTTATT TCCTAATACT AAGATATAGG 960GGCACTAAAC ACTTTATATC AGGTCCTATT 1020GACTTCTCGT TAGAGATGCT CTTAGCGAGT 1080GTTTAGTTGG GGGCTTTAAT ATGTTTAGTT 1140CTTTGGATTA TGACAACGAC CACTATCCAA 1200CTATCTGATC TATTAAATTA TGATCCAACC 12 60CACCACCAGC CCAAGTATCT TTATGAAAAA 1320GGATTATAAT TTAACGTATC AGATGGTTCG 1380GCATAACAGT CGTTCTCTTT GGTTATATAA 14 4 0AAATGGTACA AACTAGTAGT GAACACATCC 1500TATCCGAATT CTTAGAACAA ATTTCATATT 1560AATCTTTTTT ATATTATCAA GCATATTATT 1620TTATCCATTA TTTGTTCCCT AGAATTTAAA 1680TTCGTATTCA AATTTTTACA TCTATTATTT 174 0TTTTTATGAA TCTTTACAAG GTTAATGTTA 1800TCTATGTCTT ATTTGTCCGC GATTGAAGAA 18 60ACATCTGTTT CCACTCCTAC CTATCTGACC 1920TACAAAATCA CTCCCTACCT ATCTGACCTC 1980TGTCTATGGT ACAAAACCAA GTGTTTTATA 204 0CATCCTTGAC ACACAACATA CTCCTTGTGG 2100GGGTGGACCG TTGCATGACA CGACTTATCC 2160TGGGGGTCCG GGCTAGTCTA GCCTGATCAT 2220AACGGGATAG CCCAACATGT CCCTTTTTGT 2280ATGTAAAAAA ACAAAAGGTA TGTGTGTTAT 2340AAGCTAGCAA CTATGGTTTA AATCATACAT 2400CGATATGGGC CTTCTAGGCA CGTCGAGTGT 2 4 60ACTGGTCTAG CAGGCCGTAC CTAGGTCTTT 2520TGGCTAATCT GTACGGTACC GATACTGTCC 2580ΑΑΤΤΤΑΤAAT TGAGGAGGAA TGTACTCATG 264 0TCAGCGATAG ATTTCAACGC AACGGTGAGC 2700GGGGGGGGGA AGGCCACATC AAAGGCGAGG 27 60GCCAAGTCCA GCAGCTAGCA ATGAAAGGGT 2820ATCATCCCCT CAATAATCCG GTGCTCTCTT 2880TACACATGTC TGGTCTGACG AACAAATCAA 294 0CACTGTCATG ACATGTGCTC TGCCTCTGCT 3000TCTTTCTTGT GCTAGAGGCG AGTCAAACAA 3060TAAGGGTCAC ATGACCCTGT TCCCTATCGC 3120TAGTTACCAA CGACCCATTC CCCCTGACAG ATACTTACGA CGCGTCCGTA CGCGGCAGGC 3180CTCGGCAGTT CGGCATCACC AGCACCGGCG CCGGCATTCG CCCCCTGCCA GCCGGT 3236

<210> 72

<211> 1000

<212> DNA

<213> Zea mays

<400> 72

TGGTCCTTGT TTGATTTACT TCCAGGATTACTATTGACGT CACGTGCTTA TGTATTATAACTAATAATAA TCTGTGTTGT TTGTTTATCTGGGCGTCCAA TGTGTCGTCA AGTAGTGTTATGGAAGGTGT TCCTTTTCCT TAAGTTGTTAAATAGGACCT ATAATGGATT CTATTTTGAATAATGGGGAT TCTATTTTAC ΑΑΑΑΑΑΑΤΑΤCTAAATATAG TATCTCATAT AATAGAGCTCTTTATTTACT AAATAACATG ATTTATTTTCAAACGTTTTT GTGGTACTAA ACACTTTAAAATATAAGACT TCTGGTTAGA GATGCTCTTACTTTGTGGAT TGTGGAAGGT GTTACTTTTCTAGAGCATCT CTAACAGGAG CCTTAACGGAAAAAACATAC TTTAATAGGG GTCCTATTTTCACTCCTCGG GTCCTAAATA TAATATCTCACTATCCCTAG GTTTTTATTC CCTAAATAACGCTCAACTAT TGGAGTTGCA AATGTTTTTT

TATAATCCAG CTTATGGATT ATATAAGTAC 60

TAATCTAGGT ATATAGATTA TATAATCTAT 120

CTCAAAACAA ACAGGTCCTA AAATGGTCCC 180

AGCTAAATCG ACATTTCTTT GTGGGTTGTG 24 0

GTTGTGCAAG GTGTTCCTTA GAGCATCTCC 300

TTATAAGACT CTAACAACAA AAGCATACTT 360

CAAATGATTA TATGGTCGAT TCCTCGGGTC 4 20

TATCCTCATT TTATATACTA TTTTTAAGTT 480

TAATACTATG AACTCAACTA TTAGAGCTGT 54 0

TCAGGTCCTA TTTTAATTTG AAGGACTTAA 600

GCGAGTGTTT GTGCATGATT GCTATTTAGT 660

CTCAAGTTGT TAGTTGTGCA AGGTGTTTCT 720

ATCTATTTTG AAGTATAGTA CTTTAACACC 780

ACAAAAAAAT TATCAAATGA TTATAAGGTC 84 0

TATACTAGAG CTCTATCCTC ΑΤΤΤΤΑΤΑΤΑ 900

ATGATTTATT TCCTAATACT AAGATATAGG 960

GGCACTAAAC 1000

<210> 73

<211> 2236

<212> DNA

<213> Zea mays

<400> 73

ACTTTATATC AGGTCCTATT ΤΤΑΑΤΤΤΤΑΑTAGAGATGCT CTTAGCGAGT GTTTGTGCATGGGCTTTAAT ATGTTTAGTT GAAGTTCTAGTGACAACGAC CACTATCCAA GCAGTCTTTGTATTAAATTA TGATCCAACC GTTATGTCATCCAAGTATCT TTATGAAAAA CCCTAACAAATTAACGTATC AGATGGTTCG CTTGCATGCTCGTTCTCTTT GGTTATATAA TGCTTTAGTAAACTAGTAGT GAACACATCC TCCCTACCTACTTAGAACAA ATTTCATATT TTAAAATAGAATATTATCAA GCATATTATT ACACATAAGATTTGTTCCCT AGAATTTAAA AAGTGAAAAAAATTTTTACA TCTATTATTT GAGAATATATTCTTTACAAG GTTAATGTTA AATACATGACATTTGTCCGC GATTGAAGAA AAATGACAAACCACTCCTAC CTATCTGACC TCCTATTTCACTCCCTACCT ATCTGACCTC CTATTTCAAAACAAAACCAA GTGTTTTATA CATCTATTTGACACAACATA CTCCTTGTGG CTATAAATGTTTGCATGACA CGACTTATCC CAAGACGAGCGGCTAGTCTA GCCTGATCAT CGGGTCACACCCCAACATGT CCCTTTTTGT CATGCATATAACAAAAGGTA TGTGTGTTAT GTTGGTTAGACTATGGTTTA AATCATACAT ATACACATTTCTTCTAGGCA CGTCGAGTGT GACGGGCCAGCAGGCCGTAC CTAGGTCTTT CTCGTGGGCC

TTTGAAGGAC TCAAATATAG GACTTCTCGT 60

GATTGCTATT TATGTCTGTA GTTTAGTTGG 120

TATTTTTTAG GTTCTCCACT CTTTGGATTA 180

AGTGCAAACG CGCGAGCAAA CTATCTGATC 240

ATTGAAGACT TAAACCCTTT CACCACCAGC 300

CCACAATTGC ATCTATGGTT GGATTATAAT 360

TACATATCTA GAAACTGTTT GCATAACAGT 420

ATCATCAGCC AAGTGTAAAC AAATGGTACA 4 80

TCTCTAGGGG TGTAACTAGA TATCCGAATT 54 0

TATGCTTCAA AATTTATGCT AATCTTTTTT 600

ATAAAATTTT GTATAGAATT TTATCCATTA 660

ACATTCGAAT CTGTATCAGT TTCGTATTCA 720

ATGATAAATT TGAGGTTTAG TTTTTATGAA 7 80

TATGGATTTA CATAGTAAAT TCTATGTCTT 840

AAGATCTGAC ATTCGAATAA ACATCTGTTT 900

AACTCCACTT TGTAACACGG TACAAAATCA 960

CTCCACTCAG TAAACAATAT TGTCTATGGT 1020

CACGATCTGC TCGAGTCAGG CATCCTTGAC 1080

CCAAATAGAG CAGACCTAAT GGGTGGACCG 1140

ACAGTTCGCC CCATTGGTCA TGGGGGTCCG 1200

TTAGGCCACA GGTGTGCCAC AACGGGATAG 1260

TCTATATTAT AGTTAGTATA ATGTAAAAAA 1320

TGTGTTTAAA TAACTCTTTA AAGCTAGCAA 1380

ΤΤΑΤΤΤΤΑΤΤ ΤΤΤΑΤΤΤΑΑΑ CGATATGGGC 14 40

TGAGATGACA CATTATAATT ACTGGTCTAG 1500

AAGACTAAGG GTTGGCCCGT TGGCTAATCT 1560GTACGGTACC GATACTGTCC TAATTCATTT GAACACCTGT AGAAGAGGGG AATTTATAAT 1620TGAGGAGGAA TGTACTCATG CGGTACACCA GGGGAATTGT TTTGTTGTGC TCAGCGATAG 1680ATTTCAACGC AACGGTGAGC CAGTTTCACT AAAAAAAGGG GGGGGGGGGG GGGGGGGGGA 174 0AGGCCACATC AAAGGCGAGG TGCTGACGAG CAGAAGATGC TAGCAGTGAC GCCAAGTCCA 1800GCAGCTAGCA ATGAAAGGGT ACTCGGGATT TAACAATGCC TAGAGACGGC ATCATCCCCT 18 60CAATAATCCG GTGCTCTCTT TTTGTTTATT CACCAGTTGG CGTAGCTATA TACACATGTC 1920TGGTCTGACG AACAAATCAA GGGATCGCTA GCTCGGGCTA GCCTTCCTAT CACTGTCATG 1980ACATGTGCTC TGCCTCTGCT GGTTGATAAG CCGTGCGCCT TCTCGCTAAT TCTTTCTTGT 204 0GCTAGAGGCG AGTCAAACAA ACGCTGCACC TCGTAGCCCT TAATCTGCGC TAAGGGTCAC 2100ATGACCCTGT TCCCTATCGC TAGTTACCAA CGACCCATTC CCCCTGACAG ATACTTACGA 2160CGCGTCCGTA CGCGGCAGGC CTCGGCAGTT CGGCATCACC AGCACCGGCG CCGGCATTCG 2220CCCCCTGCCA GCCGGT 2236

<210> 74

<211> 1237

<212> DNA

<213> Zea mays

<400> 74

GTAAACAATA TTGTCTATGG TACAAAACCA AGTGTTTTAT ACATCTATTT GCACGATCTG 60CTCGAGTCAG GCATCCTTGA CACACAACAT ACTCCTTGTG GCTATAAATG TCCAAATAGA 120GCAGACCTAA TGGGTGGACC GTTGCATGAC ACGACTTATC CCAAGACGAG CACAGTTCGC 180CCCATTGGTC ATGGGGGTCC GGGCTAGTCT AGCCTGATCA TCGGGTCACA CTTAGGCCAC 24 0AGGTGTGCCA CAACGGGATA GCCCAACATG TCCCTTTTTG TCATGCATAT ATCTATATTA 300TAGTTAGTAT AATGTAAAAA AACAAAAGGT ATGTGTGTTA TGTTGGTTAG ATGTGTTTAA 360ATAACTCTTT AAAGCTAGCA ACTATGGTTT AAATCATACA TATACACATT ΤΤΤΑΤΤΤΤΑΤ 4 20TTTTATTTAA ACGATATGGG CCTTCTAGGC ACGTCGAGTG TGACGGGCCA GTGAGATGAC 4 80ACATTATAAT TACTGGTCTA GCAGGCCGTA CCTAGGTCTT TCTCGTGGGC CAAGACTAAG 54 0GGTTGGCCCG TTGGCTAATC TGTACGGTAC CGATACTGTC CTAATTCATT TGAACACCTG 600TAGAAGAGGG GAATTTATAA TTGAGGAGGA ATGTACTCAT GCGGTACACC AGGGGAATTG 660TTTTGTTGTG CTCAGCGATA GATTTCAACG CAACGGTGAG CCAGTTTCAC TAAAAAAAGG 720GGGGGGGGGG GGGGGGGGGG AAGGCCACAT CAAAGGCGAG GTGCTGACGA GCAGAAGATG 780CTAGCAGTGA CGCCAAGTCC AGCAGCTAGC AATGAAAGGG TACTCGGGAT TTAACAATGC 84 0CTAGAGACGG CATCATCCCC TCAATAATCC GGTGCTCTCT TTTTGTTTAT TCACCAGTTG 900GCGTAGCTAT ATACACATGT CTGGTCTGAC GAACAAATCA AGGGATCGCT AGCTCGGGCT 960AGCCTTCCTA TCACTGTCAT GACATGTGCT CTGCCTCTGC TGGTTGATAA GCCGTGCGCC 1020TTCTCGCTAA TTCTTTCTTG TGCTAGAGGC GAGTCAAACA AACGCTGCAC CTCGTAGCCC 1080TTAATCTGCG CTAAGGGTCA CATGACCCTG TTCCCTATCG CTAGTTACCA ACGACCCATT 114 0CCCCCTGACA GATACTTACG ACGCGTCCGT ACGCGGCAGG CCTCGGCAGT TCGGCATCAC 1200CAGCACCGGC GCCGGCATTC GCCCCCTGCC AGCCGGT 1237

<210> 75

<211> 21

<212> DNA

<213> PRIMER

<4 00> 75

GCCGTGCGCC TTCTCGCTAA T 21

<210> 76

<211> 24

<212> DNA

<213> PRIMER

<4 00> 76

GCGAGGAGTA CGACTGCCAA TCAA 24

<210> 77<211> 32<212> DNA

<213> PRIMER

<400> 77

TTCGGATCCT GGTCCTTGTT TGATTTACTT

<210> 78

<211> 27

<212> DNA

<213> PRIMER

<4 00> 78

GGCAAGCTTC GGTCGTCCCT TGCTATC

<210> 79

<211> 18

<212> DNA

<213> PRIMER

<400> 79

TGTAAAACGA CGGCCAGT

<210> 80

<211> 19

<212> DNA

<213> PRIMER

<4 00> 80

GGAAACAGCT ATGACCATG

<210> 81<211> 24<212> DNA

<213> PRIMER

<400> 81

TCAAATGATT ATATGGTCGA TTCC

<210> 82

<211> 20

<212> DNA

<213> PRIMER

<400> 82

CGAGCAGATC GTGCAAATAG

<210> 83

<211> 18

<212> DNA

<213> PRIMER

<400> 83

TGCTAGCTGC TGGACTTG

<210> 84

<211> 24

<212> DNA

<213> PRIMER<400> 84

TTGATTGGCA GTCGTACTCC TCGC 24

<210> 85

<211> 2777

<212> DNA

<213> VETOR

<400> 85

GAAAGGCCCA GTCTTCCGAC TGAGCCTTTCCTCGCGTTAA CGCTAGCATG GATGTTTTCCCTTAAGCTCG GGCCCGCGTT AACGCTACCATGATAGTGAC CTGTTCGTTG CAACAAATTGTGTATAGAAA AGTTGGGCCG AATTCGAGCTACCGAATTCG AGCTCGGTAC CCTGGGATCCCACCCGGTCC GGGCCTAGAA GGCCAGCTTCGTAAAATGAT ATAAATATCA ATATATTAAAATACTGTAAA ACACAACATA TGCAGTCACTCCTGTAGAAT TCGAGCTCTA GAGCTGCAGGATTACATGGT CATAGCTGTT TCCTGGCAGCACATTGCACA AGATAAAAAT ATATCATCATGTAATACAAG GGGTGTTATG AGCCATATTCCCAACATGGA TGCTGATTTA TATGGGTATAGTGCGACAAT CTATCGCTTG TATGGGAAGCGCAAAGGTAG CGTTGCCAAT GATGTTACAGAATTTATGCC TCTTCCGACC ATCAAGCATTTCACCACTGC GATCCCCGGA AAAACAGCATGTGAAAATAT TGTTGATGCG CTGGCAGTGTGTAATTGTCC TTTTAACAGC GATCGCGTATATAACGGTTT GGTTGATGCG AGTGATTTTGAAGTCTGGAA AGAAATGCAT AAACTTTTGCGTGATTTCTC ACTTGATAAC CTTATTTTTGTTGGACGAGT CGGAATCGCA GACCGATACCGTGAGTTTTC TCCTTCATTA CAGAAACGGCATATGAATAA ATTGCAGTTT CATTTGATGCATTGGTTGTA ACACTGGCAG AGCATTACGCCAAAATCCCT TAACGTGAGT TACGCGTCGTTCAAAGGATC TTCTTGAGAT CCTTTTTTTCAACCACCGCT ACCAGCGGTG GTTTGTTTGCAGGTAACTGG CTTCAGCAGA GCGCAGATACTAGGCCACCA CTTCAAGAAC TCTGTAGCACTACCAGTGGC TGCTGCCAGT GGCGATAAGTAGTTACCGGA TAAGGCGCAG CGGTCGGGCTTGGAGCGAAC GACCTACACC GAACTGAGATCGCTTCCCGA AGGGAGAAAG GCGGACAGGTAGCGCACGAG GGAGCTTCCA GGGGGAAACGGCCACCTCTG ACTTGAGCGT CGATTTTTGTAAAACGCCAG CAACGCGGCC TTTTTACGGTTGTTCTTTCC TGCGTTATCC CCTGATTCTGCTGATACCGC TCGCCGCAGC CGAACGACCGAAGAGCGCCC AATACGCAAA CCGCCTCTCCGGCACGACAG GTTTCCCGAC TGGAAAGCGGCCGCTAGCCA GGAAGAGTTT GTAGAAACGCCTTAGTTTGA TGCCTGGCAG TTTATGGCGGCTTCACAACG TTCAAATCCG CTCCCGGCGGCAAACAACAG ATAAAAC

GTTTTATTTG ATGCCTGGCA GTTCCCTACT 60CAGTCACGAC GTTGTAAAAC GACGGCCAGT 120TGGAGCTCCA AATAATGATT TTATTTTGAC 180ATAAGCAATG CTTTTTTATA ATGCCAACTT 240CGGTACGGCC AGAATGGCCC GGACCGGGTT 300GATATCGATG GGCCCTGGCC GAAGCTTGGT 360AAGTTTGTAC AAAAAAGTTG AACGAGAAAC 420TTAGATTTTG CATAAAAAAC AGACTACATA 480ATGAATCAAC TACTTAGATG GTATTAGTGA 540GCGGCCGCGA TATCCCCTAT AGTGAGTCGT 600TCTGGCCCGT GTCTCAAAAT CTCTGATGTT 660GAACAATAAA ACTGTCTGCT TACATAAACA 720AACGGGAAAC GTCGAGGCCG CGATTAAATT 780AATGGGCTCG CGATAATGTC GGGCAATCAG 840CCGATGCGCC AGAGTTGTTT CTGAAACATG 900ATGAGATGGT CAGACTAAAC TGGCTGACGG 960TTATCCGTAC TCCTGATGAT GCATGGTTAC 1020TCCAGGTATT AGAAGAATAT CCTGATTCAG 1080TCCTGCGCCG GTTGCATTCG ATTCCTGTTT 1140TTCGTCTCGC TCAGGCGCAA TCACGAATGA 1200ATGACGAGCG TAATGGCTGG CCTGTTGAAC 1260CATTCTCACC GGATTCAGTC GTCACTCATG 1320ACGAGGGGAA ATTAATAGGT TGTATTGATG 1380AGGATCTTGC CATCCTATGG AACTGCCTCG 1440TTTTTCAAAA ATATGGTATT GATAATCCTG 1500TCGATGAGTT TTTCTAATCA GAATTGGTTA 1560TGACTTGACG GGACGGCGCA AGCTCATGAC 1620TCCACTGAGC GTCAGACCCC GTAGAAAAGA 1680TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 1740CGGATCAAGA GCTACCAACT CTTTTTCCGA 1800CAAATACTGT CCTTCTAGTG TAGCCGTAGT 1860CGCCTACATA CCTCGCTCTG CTAATCCTGT 1920CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 1980GAACGGGGGG TTCGTGCACA CAGCCCAGCT 2040ACCTACAGCG TGAGCATTGA GAAAGCGCCA 2100ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 2160CCTGGTATCT TTATAGTCCT GTCGGGTTTC 2220GATGCTCGTC AGGGGGGCGG AGCCTATGGA 2280TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 2340TGGATAACCG TATTACCGCC TTTGAGTGAG 2400AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 2460CCGCGCGTTG GCCGATTCAT TAATGCAGCT 2520GCAGTGAGCG CAACGCAATT AATACGCGTA 2580AAAAAGGCCA TCCGTCAGGA TGGCCTTCTG 2640GCGTCCTGCC CGCCACCCTC CGGGCCGTTG 2700ATTTGTCCTA CTCAGGAGAG CGTTCACCGA 2760

2777

<210> 86<211> 6377<212> DNA<213> VETOR

<400> 86

GAAAGGCCCA GTCTTCCGAC TGAGCCTTTCCTCGCGTTAA CGCTAGCATG GATGTTTTCCCTTAAGCTCG GGCCCGCGTT AACGCTACCATGATAGTGAC CTGTTCGTTG CAACAAATTGTGTATAGAAA AGTTGGGCCG AATTCGAGCTACCGAATTCG AGCTCGGTAC CCTGGGATCCTACTTCCAGG ΑΤΤΑΤΑΤΑΑΤ CCAGCTTATGCTTATGTATT ATAATAATCT AGGTATATAGTTGTTTGTTT ATCTCTCAAA ACAAACAGGTGTCAAGTAGT GTTAAGCTAA ATCGACATTTTCCTTAAGTT GTTAGTTGTG CAAGGTGTTCGATTCTATTT TGAATTATAA GACTCTAACATTACAAAAAA ATATCAAATG ATTATATGGTATATAATAGA GCTCTATCCT CATTTTATATCATGATTTAT TTTCTAATAC TATGAACTCACTAAACACTT TAAATCAGGT CCTATTTTAATAGAGATGCT CTTAGCGAGT GTTTGTGCATAGGTGTTACT TTTCCTCAAG TTGTTAGTTGGGAGCCTTAA CGGAATCTAT TTTGAAGTATAGGGGTCCTA TTTTACAAAA AAATTATCAAAATATAATAT CTCATATACT AGAGCTCTATATTCCCTAAA TAACATGATT TATTTCCTAATGCAAATGTT TTTTGGCACT AAACACTTTAGGACTCAAAT ATAGGACTTC TCGTTAGAGATATTTATGTC TGTAGTTTAG TTGGGGGCTTTTAGGTTCTC CACTCTTTGG ATTATGACAAAACGCGCGAG CAAACTATCT GATCTATTAAGACTTAAACC CTTTCACCAC CAGCCCAAGTTTGCATCTAT GGTTGGATTA TAATTTAACGTCTAGAAACT GTTTGCATAA CAGTCGTTCTAGCCAAGTGT AAACAAATGG TACAAACTAGGGGGTGTAAC TAGATATCCG AATTCTTAGATCAAAATTTA TGCTAATCTT ΤΤΤΤΑΤΑΤΤΑTTTTGTATAG AATTTTATCC ATTATTTGTTGAATCTGTAT CAGTTTCGTA TTCAAATTTTAATTTGAGGT TTAGTTTTTA TGAATCTTTATTTACATAGT AAATTCTATG TCTTATTTGTTGACATTCGA ATAAACATCT GTTTCCACTCACTTTGTAAC ACGGTACAAA ATCACTCCCTTCAGTAAACA ATATTGTCTA TGGTACAAAACTGCTCGAGT CAGGCATCCT TGACACACAAAGAGCAGACC TAATGGGTGG ACCGTTGCATCGCCCCATTG GTCATGGGGG TCCGGGCTAGCACAGGTGTG CCACAACGGG ATAGCCCAACTTATAGTTAG TATAATGTAA AAAAACAAAATAAATAACTC TTTAAAGCTA GCAACTATGGΤΑΤΤΤΤΤΑΤΤ TAAACGATAT GGGCCTTCTAGACACATTAT AATTACTGGT CTAGCAGGCCAAGGGTTGGC CCGTTGGCTA ATCTGTACGGCTGTAGAAGA GGGGAATTTA TAATTGAGGATTGTTTTGTT GTGCTCAGCG ATAGATTTCAAGGGGGGGGG GGGGGGGGGG GGGAAGGCCAATGCTAGCAG TGACGCCAAG TCCAGCAGCTTGCCTAGAGA CGGCATCATC CCCTCAATAATTGGCGTAGC TATATACACA TGTCTGGTCT

GTTTTATTTG ATGCCTGGCA GTTCCCTACT 60CAGTCACGAC GTTGTAAAAC GACGGCCAGT 120TGGAGCTCCA AATAATGATT TTATTTTGAC 180ATAAGCAATG CTTTTTTATA ATGCCAACTT 240CGGTACGGCC AGAATGGCCC GGACCGGGTT 300GCAAGGGACG ACCGTGGTCC TTGTTTGATT 360GATTATATAA GTACCTATTG ACGTCACGTG 420ΑΤΤΑΤΑΤΑΑΤ CTATCTAATA ATAATCTGTG 4 80CCTAAAATGG TCCCGGGCGT CCAATGTGTC 54 0CTTTGTGGGT TGTGTGGAAG GTGTTCCTTT 600CTTAGAGCAT CTCCAATAGG ACCTATAATG 660ACAAAAGCAT ACTTTAATGG GGATTCTATT 720CGATTCCTCG GGTCCTAAAT ATAGTATCTC 7 80ACTATTTTTA AGTTTTTATT TACTAAATAA 84 0ACTATTAGAG CTGTAAACGT TTTTGTGGTA 900TTTGAAGGAC TTAAATATAA GACTTCTGGT 960GATTGCTATT TAGTCTTTGT GGATTGTGGA 1020TGCAAGGTGT TTCTTAGAGC ATCTCTAACA 1080AGTACTTTAA CACCAAAAAC ATACTTTAAT 114 0ATGATTATAA GGTCCACTCC TCGGGTCCTA 1200CCTCATTTTA TATACTATCC CTAGGTTTTT 1260TACTAAGATA TAGGGCTCAA CTATTGGAGT 1320TATCAGGTCC ΤΑΤΤΤΤΑΑΤΤ TTAATTTGAA 1380TGCTCTTAGC GAGTGTTTGT GCATGATTGC 14 40TAATATGTTT AGTTGAAGTT CTAGTATTTT 1500CGACCACTAT CCAAGCAGTC TTTGAGTGCA 1560ATTATGATCC AACCGTTATG TCATATTGAA 1620ATCTTTATGA AAAACCCTAA CAAACCACAA 1680TATCAGATGG TTCGCTTGCA TGCTTACATA 174 0CTTTGGTTAT ATAATGCTTT AGTAATCATC 1800TAGTGAACAC ATCCTCCCTA CCTATCTCTA 18 60ACAAATTTCA ΤΑΤΤΤΤΑΑΑΑ TAGATATGCT 1920TCAAGCATAT TATTACACAT AAGAATAAAA 1980CCCTAGAATT TAAAAAGTGA AAAAACATTC 204 0TACATCTATT ATTTGAGAAT ATATATGATA 2100CAAGGTTAAT GTTAAATACA TGACTATGGA 2160CCGCGATTGA AGAAAAATGA CAAAAAGATC 2220CTACCTATCT GACCTCCTAT TTCAAACTCC 2280ACCTATCTGA CCTCCTATTT CAAACTCCAC 234 0CCAAGTGTTT TATACATCTA TTTGCACGAT 2 4 00CATACTCCTT GTGGCTATAA ATGTCCAAAT 24 60GACACGACTT ATCCCAAGAC GAGCACAGTT 2520TCTAGCCTGA TCATCGGGTC ACACTTAGGC 2580ATGTCCCTTT TTGTCATGCA TATATCTATA 2 64 0GGTATGTGTG TTATGTTGGT TAGATGTGTT 2700TTTAAATCAT ACATATACAC ΑΤΤΤΤΤΑΤΤΤ 27 60GGCACGTCGA GTGTGACGGG CCAGTGAGAT 2820GTACCTAGGT CTTTCTCGTG GGCCAAGACT 2880TACCGATACT GTCCTAATTC ATTTGAACAC 2 94 0GGAATGTACT CATGCGGTAC ACCAGGGGAA 3000ACGCAACGGT GAGCCAGTTT CACTAAAAAA 3060CATCAAAGGC GAGGTGCTGA CGAGCAGAAG 3120AGCAATGAAA GGGTACTCGG GATTTAACAA 3180TCCGGTGCTC TCTTTTTGTT TATTCACCAG 324 0GACGAACAAA TCAAGGGATC GCTAGCTCGG 3300GCTAGCCTTC CTATCACTGT CATGACATGTGCCTTCTCGC TAATTCTTTC TTGTGCTAGACCCTTAATCT GCGCTAAGGG TCACATGACCATTCCCCCTG ACAGATACTT ACGACGCGTCCACCAGCACC GGCGCCGGCA TTCGCCCCCTGTCGGCCGCA GTTGCCGCAT CCCAAACGCCATGAAGTTGC TGCCCCTGGC TTCGTAAAGCTACTCCTCGC TCATAGGCCG ACACGGCCGCACCCCCGCCA CGCGCCTATA TATGTTCGCGAGCCACAACC CCGAGCTCAG ATCCCTCGCCACAGCCAGTG TGGGCCAGAC GGACACCGCCCACCCGGTCC GGGCCTAGAA GGCCAGCTTCGTAAAATGAT ATAAATATCA ATATATTAAAATACTGTAAA ACACAACATA TGCAGTCACTCCTGTAGAAT TCGAGCTCTA GAGCTGCAGGATTACATGGT CATAGCTGTT TCCTGGCAGCACATTGCACA AGATAAAAAT ATATCATCATGTAATACAAG GGGTGTTATG AGCCATATTCCCAACATGGA TGCTGATTTA TATGGGTATAGTGCGACAAT CTATCGCTTG TATGGGAAGCGCAAAGGTAG CGTTGCCAAT GATGTTACAGAATTTATGCC TCTTCCGACC ATCAAGCATTTCACCACTGC GATCCCCGGA AAAACAGCATGTGAAAATAT TGTTGATGCG CTGGCAGTGTGTAATTGTCC TTTTAACAGC GATCGCGTATATAACGGTTT GGTTGATGCG AGTGATTTTGAAGTCTGGAA AGAAATGCAT AAACTTTTGCGTGATTTCTC ACTTGATAAC CTTATTTTTGTTGGACGAGT CGGAATCGCA GACCGATACCGTGAGTTTTC TCCTTCATTA CAGAAACGGCATATGAATAA ATTGCAGTTT CATTTGATGCATTGGTTGTA ACACTGGCAG AGCATTACGCCAAAATCCCT TAACGTGAGT TACGCGTCGTTCAAAGGATC TTCTTGAGAT CCTTTTTTTCAACCACCGCT ACCAGCGGTG GTTTGTTTGCAGGTAACTGG CTTCAGCAGA GCGCAGATACTAGGCCACCA CTTCAAGAAC TCTGTAGCACTACCAGTGGC TGCTGCCAGT GGCGATAAGTAGTTACCGGA TAAGGCGCAG CGGTCGGGCTTGGAGCGAAC GACCTACACC GAACTGAGATCGCTTCCCGA AGGGAGAAAG GCGGACAGGTAGCGCACGAG GGAGCTTCCA GGGGGAAACGGCCACCTCTG ACTTGAGCGT CGATTTTTGTAAAACGCCAG CAACGCGGCC TTTTTACGGTTGTTCTTTCC TGCGTTATCC CCTGATTCTGCTGATACCGC TCGCCGCAGC CGAACGACCGAAGAGCGCCC AATACGCAAA CCGCCTCTCCGGCACGACAG GTTTCCCGAC TGGAAAGCGGCCGCTAGCCA GGAAGAGTTT GTAGAAACGCCTTAGTTTGA TGCCTGGCAG TTTATGGCGGCTTCACAACG TTCAAATCCG CTCCCGGCGGCAAACAACAG ATAAAACGCTCTGCCTC TGCTGGTTGA TAAGCCGTGC 3360GGCGAGTCAA ACAAACGCTG CACCTCGTAG 34 20CTGTTCCCTA TCGCTAGTTA CCAACGACCC 3480CGTACGCGGC AGGCCTCGGC AGTTCGGCAT 354 0GCCAGCCGGT TCGCAGATTC GCAGGGCGGA 3600CGGGAACCTT TGGGGCCCCT CTACGAGCAA 3660TCTGACTTTT GATCACTTGA TTGGCAGTCG 37 20AAAGTCAACT ACCCGCTCCG CCATCCTTCA 37 80GCCATGTCCG TACTAGTCCT CCAACCCACA 384 0TCGTGTCGTG TCTCCGGTCG ACGACGACCA 3900GAGCTATAGC GCTTGGTGAT AAAGCTTGGT 3960AAGTTTGTAC AAAAAAGTTG AACGAGAAAC 4 020TTAGATTTTG CATAAAAAAC AGACTACATA 4 080ATGAATCAAC TACTTAGATG GTATTAGTGA 414 0GCGGCCGCGA TATCCCCTAT AGTGAGTCGT 4 200TCTGGCCCGT GTCTCAAAAT CTCTGATGTT 4 2 60GAACAATAAA ACTGTCTGCT TACATAAACA 4 320AACGGGAAAC GTCGAGGCCG CGATTAAATT 4 380AATGGGCTCG CGATAATGTC GGGCAATCAG 4 4 40CCGATGCGCC AGAGTTGTTT CTGAAACATG 4 500ATGAGATGGT CAGACTAAAC TGGCTGACGG 4 560TTATCCGTAC TCCTGATGAT GCATGGTTAC 4 620TCCAGGTATT AGAAGAATAT CCTGATTCAG 4 680TCCTGCGCCG GTTGCATTCG ATTCCTGTTT 47 4 0TTCGTCTCGC TCAGGCGCAA TCACGAATGA 4 800ATGACGAGCG TAATGGCTGG CCTGTTGAAC 4 8 60CATTCTCACC GGATTCAGTC GTCACTCATG 4 920ACGAGGGGAA ATTAATAGGT TGTATTGATG 4 98 0AGGATCTTGC CATCCTATGG AACTGCCTCG 504 0TTTTTCAAAA ATATGGTATT GATAATCCTG 5100TCGATGAGTT TTTCTAATCA GAATTGGTTA 5160TGACTTGACG GGACGGCGCA AGCTCATGAC 5220TCCACTGAGC GTCAGACCCC GTAGAAAAGA 5280TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 534 0CGGATCAAGA GCTACCAACT CTTTTTCCGA 54 00CAAATACTGT CCTTCTAGTG TAGCCGTAGT 54 60CGCCTACATA CCTCGCTCTG CTAATCCTGT 5520CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 5580GAACGGGGGG TTCGTGCACA CAGCCCAGCT 564 0ACCTACAGCG TGAGCATTGA GAAAGCGCCA 57 00ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 57 60CCTGGTATCT TTATAGTCCT GTCGGGTTTC 5820GATGCTCGTC AGGGGGGCGG AGCCTATGGA 5880TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 594 0TGGATAACCG TATTACCGCC TTTGAGTGAG 6000AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 6060CCGCGCGTTG GCCGATTCAT TAATGCAGCT 6120GCAGTGAGCG CAACGCAATT AATACGCGTA 6180AAAAAGGCCA TCCGTCAGGA TGGCCTTCTG 6240GCGTCCTGCC CGCCACCCTC CGGGCCGTTG 6300ATTTGTCCTA CTCAGGAGAG CGTTCACCGA 6360

6377

<210> 87

<211> 17777

<212> DNA

<213> VETOR<400> 87

ATTATACAAA GTTGATAGAT ATCGGACCGAGCCTGCAGTG CAGCGTGACC CGGTCGTGCCTAAGTTATAA AAAATTACCA CATATTTTTTTATCTTTATA CATATATTTA AACTTTACTCAATATCAGTG TTTTAGAGAA TCATATAAATGAGTATTTTG ACAACAGGAC TCTACAGTTTTTTTTTGCAA ATAGCTTCAC CTATATAATAGGGTTTAGGG TTAATGGTTT TTATAGACTATTAGCCTCTA AATTAAGAAA ACTAAAACTCTATAAAATAG AATAAAATAA AGTGACTAAAAAAACTAAGG AAACATTTTT CTTGTTTCGAGACGAGTCTA ACGGACACCA ACCAGCGAACGACGGCACGG CATCTCTGTC GCTGCCTCTGGGACTTGCTC CGCTGTCGGC ATCCAGAAATACGGCAGGCG GCCTCCTCCT CCTCTCACGGCCGCTCCTTC GCTTTCCCTT CCTCGCCCGCCTTTCCCCAA CCTCGTGTTG TTCGGAGCGCCACCCGTCGG CACCTCCGCT TCAAGGTACGCTTCTCTAGA TCGGCGTTCC GGTCCATGCACATGTTTGTG TTAGATCCGT GTTTGTGTTAGCGACCTGTA CGTCAGACAC GTTCTGATTGCCTGGGATGG CTCTAGCCGT TCCGCAGACGTGCATAGGGT TTGGTTTGCC CTTTTCCTTTGGGTCATCTT TTCATGCTTT TTTTTGTCTTCGTTCTAGAT CGGAGTAGAA TTCTGTTTCACTGTATGTGT GTGCCATACA TATTCATAGTGATCTAGGAT AGGTATACAT GTTGATGCGGTTGTTCGCTT GGTTGTGATG ATGTGGTGTGGAGTAGAATA CTGTTTCAAA CTACCTGGTGGTCATACATC TTCATAGTTA CGAGTTTAAGTACATGTTGA TGTGGGTTTT ACTGATGCATTATGCTCTAA CCTTGAGTAC CTATCTATTAGATCTTGATA TACTTGGATG ATGGCATATGGCCTTCATAC GCTATTTATT TGCTTGGTACTTGGTGTTAC TTCTGCAGGT CGACTTTAACCCCGAGCGCC GCCCCGTCGA GATCCGCCCGGACATCGTGA ACCACTACAT CGAGACCTCCCCGCAGGAGT GGATCGACGA CCTGGAGCGCGAGGTGGAGG GCGTGGTGGC CGGCATCGCCTACGACTGGA CCGTGGAGTC CACCGTGTACGGCTCCACCC TCTACACCCA CCTCCTCAAGGTGGCCGTGA TCGGCCTCCC GAACGACCCGACCGCCCGCG GCACCCTCCG CGCCGCCGGCTTCTGGCAGC GCGACTTCGA GCTGCCGGCCATCTGAGTCG AAACCTAGAC TTGTCCATCTAATAAAAGGA TGCACACATA GTGACATGCTTGTTATGTGT AATTACTAGT TATCTGAATACCTAAATGAA TGTCACGTGT CTTTATAATTAAATCCATAT ACATATAAAT ATTAATCATAAAATCTAGTC TAGGTGTGTT TTGCGAATTGTTCCGATTAA TCGTGGCCTC TTGCTCTTCAGCTACTAGAC AATTCAGTAC ATTAAAAACGTCAATTTGTT TACACCACAA TATATCCTGCAGCTCGGCAC AAAATCACCA CTCGATACAGGGAGAGCCGT TGTAAGGCGG CAGACTTTGCCAACTAAGCT GCCGGGTTTG AAACACGGATACGATGACAG AGCGTTGCTG CCTGTGATCAATCAGCCTTC TTATTCATTT CTCGCTTAAC

TTAAACTTTA ATTCGGTCCG AAGCTTGCAT 60

CCTCTCTAGA GATAATGAGC ATTGCATGTC 120

TTGTCACACT TGTTTGAAGT GCAGTTTATC 180

TACGAATAAT ATAATCTATA GTACTACAAT 240

GAACAGTTAG ACATGGTCTA AAGGACAATT 300

TATCTTTTTA GTGTGCATGT GTTCTCCTTT 360

CTTCATCCAT TTTATTAGTA CATCCATTTA 4 20

ATTTTTTTAG TACATCTATT TTATTCTATT 4 80

TATTTTAGTT TTTTTATTTA ATAATTTAGA 540

AATTAAACAA ATACCCTTTA AGAAATTAAA 600

GTAGATAATG CCAGCCTGTT AAACGCCGTC 660

CAGCAGCGTC GCGTCGGGCC AAGCGAAGCA 720

GACCCCTCTC GAGAGTTCCG CTCCACCGTT 7 80

TGCGTGGCGG AGCGGCAGAC GTGAGCCGGC 840

CACCGGCAGC TACGGGGGAT TCCTTTCCCA 900

CGTAATAAAT AGACACCCCC TCCACACCCT 960

ACACACACAC AACCAGATCT CCCCCAAATC 1020

CCGCTCGTCC TCCCCCCCCC CCCTCTCTAC 1080

TGGTTAGGGC CCGGTAGTTC TACTTCTGTT 1140

GATCCGTGCT GCTAGCGTTC GTACACGGAT 1200

CTAACTTGCC AGTGTTTCTC TTTGGGGAAT 1260

GGATCGATTT CATGATTTTT TTTGTTTCGT 1320

ATTTCAATAT ATGCCGTGCA CTTGTTTGTC 1380

GGTTGTGATG ATGTGGTCTG GTTGGGCGGT 1440

AACTACCTGG TGGATTTATT AATTTTGGAT 1500

TACGAATTGA AGATGATGGA TGGAAATATC 15 60

GTTTTACTGA TGCATATACA GAGATGCTTT 1620

GTTGGGCGGT CGTTCATTCG TTCTAGATCG 1680

TATTTATTAA TTTTGGAACT GTATGTGTGT 17 4 0

ATGGATGGAA ATATCGATCT AGGATAGGTA 1800

ATACATGATG GCATATGCAG CATCTATTCA 18 60

TAATAAACAA GTATGTTTTA TAATTATTTT 1920

CAGCAGCTAT ATGTGGATTT TTTTAGCCCT 1980

TGTTTCTTTT GTCGATGCTC ACCCTGTTGT 204 0

TTAGCCTAGG ATCCACACGA CACCATGTCC 2100

GCCACCGCCG CCGACATGGC CGCCGTGTGC 2160

ACCGTGAACT TCCGCACCGA GCCGCAGACC 2220

CTCCAGGACC GCTACCCGTG GCTCGTGGCC 2280

TACGCCGGCC CGTGGAAGGC CCGCAACGCC 234 0

GTGTCCCACC GCCACCAGCG CCTCGGCCTC 2400

AGCATGGAGG CCCAGGGCTT CAAGTCCGTG 24 60

TCCGTGCGCC TCCACGAGGC CCTCGGCTAC 2520

TACAAGCACG GCGGCTGGCA CGACGTCGGC 2580

CCGCCGCGCC CGGTGCGCCC GGTGACGCAG 264 0

TCTGGATTGG CCAACTTAAT TAATGTATGA 2700

AATCACTATA ATGTGGGCAT CAAAGTTGTG 27 60

AAAGAGAAAG AGATCATCCA TATTTCTTAT 2820

CTTTGATGAA CCAGATGCAT TTCATTAACC 2880

TATAATTAAT ATCAATTGGG TTAGCAAAAC 2940

CGGCCGCCAC CGCGGTGGAG CTCGAATTCA 3000

GGATGAAGAG CTATGTTTAA ACGTGCAAGC 3060

TCCGCAATGT GTTATTAAGT TGTCTAAGCG 3120

CACCAGCCAG CCAACAGCTC CCCGACCGGC 3180

GCAGCCCATC AGTCCGGGAC GGCGTCAGCG 324 0

TCATGTTACC GATGCTATTC GGAAGAACGG 3300

GATCTCGCGG AGGGTAGCAT GTTGATTGTA 3360

AATATCATCT CCCTCGCAGA GATCCGAATT 34 20

CGTGACAGGC TGTCGATCTT GAGAACTATG 34 80CCGACATAAT AGGAAATCGC TGGATAAAGCTAGAAGTGAA CGTTGACGAT CGTCGACCGTTGAACACAGC TGGATACTTA CTTGGGCGATTTGTGTAACC GTCTCTTGGA GGTTCGTATGGATGTTGAGG CCTAACATTT TATTAGAGAGGGCCGTTATC TGTCAGGGCA AGCGAAAATTAGCTCCCATC TTTGCCGCCA TAGACGCCGCTGCCAGATGT GGAAAAGAAG TTCGTTGTCCAAGTGCGAGA CCCATTTGCG CTATATATAAAATTGGATGA ACTATTATCG TAGTTGCTCTCGTAATTGCT TATGGAGTTG TCGTAGTTGCAGTCATAGGG AAGACGAGCT TCATCCACTACCGATGCCAT CGCAAGTACG AGGCTTAGAACCAGCTCTCT AACGCTTGAG TTAAGCCGCGTTAGACATTA TTTGCCGACT ACCTTGGTGACCAACTGATC TGCGCGCGAG GCCAAGCGATCAAGTATGAC GGGCTGATAC TGGGCCGGCACCTTCGGCGC GATTTTGCCG GTTACTGCGCCATTTCGCTC ATCGCCAGCC CAGTCGGGCGGCGCCTCAAA TAGATCCTGT TCAGGAACCGCCAAGGCAAC GCTATGTTCT CTTGCTTTTGTGGCTGGCTC GAAGATACCT GCAAGAATGTCGCGCTTAGC TGGATAACGC CACGGAATGACAGCGCGGAG AATCTCGCTC TCTCCAGGGGAAGCTCGCCG CGTTGTTTCA TCAAGCCTTATGTGTGGCTT CAGGCCGCCA TCCACTGCGGGTTCGAGATG GCGCTCGATG ACGCCAACTATCACCGCTTC CCTCATGATG TTTAACTCCTCTTGAATGAA TTGTTAGGCG TCATCCTGTGTTTCGTTCGA GACTTGAGGT CTAGTTTTATAGCCACATTT TGCGTACAAA TTGCAGGCAGTGCCAAGGAG CTGTCTGCTT AGTGCCCACTCTCCTTTGCC TCGGTGCGTG TGCGACACAAATGTTGAGTT GAGTTCAATC TTCCCGACAACAGAGTCTTC ATCAGAGTCA TCATCCGAGATGGTAGATAG TTCAAAGCCT TGGTCGGATAAATGGTTCTC AGCATCCAAT GTTTCCGCCAGACGCCTAAC GCCTGGCACA GCGGATCGCATGACAGGTTT GCGAATCCGT TGCTGCCACTTAATTTATGT TAGAGGCGAA GTCTTGGGTAGGAAAGTAAA CATCACCTTC CGGCTCGATGTGATCGGGGG ATCTGCTGCC TCGCGCGTTTGCAGCTCCCG GAGACGGTCA CAGCTTGTCTTCAGGGCGCG TCAGCGGGTG TTGGCGGGTGCGATAGCGGA GTGTATACTG GCTTAACTATCACCATATGC GGTGTGAAAT ACCGCACAGATCTTCCGCTT CCTCGCTCAC TGACTCGCTGTCAGCTCACT CAAAGGCGGT AATACGGTTAAACATGTGAG CAAAAGGCCA GCAAAAGGCCTTTTTCCATA GGCTCCGCCC CCCTGACGAGTGGCGAAACC CGACAGGACT ATAAAGATACCGCTCTCCTG TTCCGACCCT GCCGCTTACCAGCGTGGCGC TTTCTCATAG CTCACGCTGTTCCAAGCTGG GCTGTGTGCA CGAACCCCCCAACTATCGTC TTGAGTCCAA CCCGGTAAGAGGTAACAGGA TTAGCAGAGC GAGGTATGTACCTAACTACG GCTACACTAG AAGGACAGTAACCTTCGGAA AAAGAGTTGG TAGCTCTTGAGGTTTTTTTG TTTGCAAGCA GCAGATTACG

CGCTGAGGAA GCTGAGTGGC GCTATTTCTT 3540

ACCCCGATGA ATTAATTCGG ACGTACGTTC 3600

TGTCATACAT GACATCAACA ATGTACCCGT 3660ACACTAGTGG TTCCCCTCAG CTTGCGACTA 3720CAGGCTAGTT GCTTAGATAC ATGATCTTCA 3780

GGCCATTTAT GACGACCAAT GCCCCGCAGA 3840

GCCCCCCTTT TGGGGTGTAG AACATCCTTT 3900

CATTGTTGGC AATGACGTAG TAGCCGGCGA 3960

GCCTACGATT TCCGTTGCGA CTATTGTCGT 4 020

CAGAGTTGTC GTAATTTGAT GGACTATTGT 4 080

TTGGAGAAAT GTCGTAGTTG GATGGGGAGT 4140

AAACAATTGG CAGGTCAGCA AGTGCCTGCC 4200

CCACCTTCAA CAGATCGCGC ATAGTCTTCC 4260

CCGCGAAGCG GCGTCGGCTT GAACGAATTG 4 320

TCTCGCCTTT CACGTAGTGA ACAAATTCTT 4 380

CTTCTTGTCC AAGATAAGCC TGCCTAGCTT 4 4 40

GGCGCTCCAT TGCCCAGTCG GCAGCGACAT 4 500

TGTACCAAAT GCGGGACAAC GTAAGCACTA 4560

GCGAGTTCCA TAGCGTTAAG GTTTCATTTA 4 620

GATCAAAGAG TTCCTCCGCC GCTGGACCTA 4 680

TCAGCAAGAT AGCCAGATCA ATGTCGATCG 4 740

CATTGCGCTG CCATTCTCCA AATTGCAGTT 4 800

TGTCGTCGTG CACAACAATG GTGACTTCTA 4 860

AAGCCGAAGT TTCCAAAAGG TCGTTGATCA 4 920

CAGTCACCGT AACCAGCAAA TCAATATCAC 4 980

AGCCGTACAA ATGTACGGCC AGCAACGTCG 504 0

CCTCTGATAG TTGAGTCGAT ACTTCGGCGA 5100

GAATTAAGCC GCGCCGCGAA GCGGTGTCGG 5160

CTCCCGAGAA CCAGTACCAG TACATCGCTG 5220

ACGTGAACAG GTCAATGCCG CCGAGAGTAA 5280

GTACATTGTT CGTTTGTGTC TCTAATCGTA 5340

TTTTCGCAAA TTCGATGAGA CTGTGCGCGA 54 00

CAATGTGTTC GATAGAGGCT AGATCGTTCC 54 60

GCTCTTGGTC GATGAATGCG CCATAGCAAG 5520

TGTAATCCTT CCGGTAGGGG CTCACACTTC 5580

GGTGCACATC GAACACTTCA CGAACAATGA 564 0

CCTGCTCAGG GATCACCGAA ATCTTCATAT 5700

AACCTGGCGC GGCTTTTGGC ACAAAAGGCG 5760

TGTTAACCCT TTTGCCAGAT TTGGTAACTA 5820

AAAACTGGCC TAAAATTGCT GGGGATTTCA 5880

TCTATTGTAG ATATATGTAG TGTATCTACT 594 0

CGGTGATGAC GGTGAAAACC TCTGACACAT 6000

GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 6060

TCGGGGCGCA GCCATGACCC AGTCACGTAG 6120

GCGGCATCAG AGCAGATTGT ACTGAGAGTG 6180

TGCGTAAGGA GAAAATACCG CATCAGGCGC 624 0

CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA 6300

TCCACAGAAT CAGGGGATAA CGCAGGAAAG 6360

AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 64 20

CATCACAAAA ATCGACGCTC AAGTCAGAGG 64 80

CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 6540

GGATACCTGT CCGCCTTTCT CCCTTCGGGA 6600

AGGTATCTCA GTTCGGTGTA GGTCGTTCGC 6660

GTTCAGCCCG ACCGCTGCGC CTTATCCGGT 6720

CACGACTTAT CGCCACTGGC AGCAGCCACT 6780

GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 684 0

TTTGGTATCT GCGCTCTGCT GAAGCCAGTT 6900

TCCGGCAAAC AAACCACCGC TGGTAGCGGT 6960

CGCAGAAAAA AAGGATCTCA AGAAGATCCT 7020TTGATCTTTT CTACGGGGTC TGACGCTCAGGTCATGAGAT TATCAAAAAG GATCTTCACCAAATCAATCT AAAGTATATA TGAGTAAACTGAGGCACCTA TCTCAGCGAT CTGTCTATTTGTGTAGATAA CTACGATACG GGAGGGCTTACGAGACCCAC GCTCACCGGC TCCAGATTTAGAGCGCAGAA GTGGTCCTGC AACTTTATCCGAAGCTAGAG TAAGTAGTTC GCCAGTTAATGGGGGGGGGG GGGGGGGGGA CTTCCATTGTACGACAGAGG CCAAAAAGCC TCGCTTTCAGATTTTAAATA AAAACATTAA GTTATGACGATTTTCATAAA TAGCGAAAAC CCGCGAGGTCAGGACCCGTA AAGTGATAAT GATTATCATCAACCACGTCA AATAATCAAT TATGACGCAGACGTAAAAAC AACTTCAGAC AATACAAATCCCCCCCCCCC CCCCCCCCTG CAGGCATCGTATTCAGCTCC GGTTCCCAAC GATCAAGGCGAGCGGTTAGC TCCTTCGGTC CTCCGATCGTACTCATGGTT ATGGCAGCAC TGCATAATTCTTCTGTGACT GGTGAGTACT CAACCAAGTCTTGCTCTTGC CCGGCGTCAA CACGGGATAAGCTCATCATT GGAAAACGTT CTTCGGGGCGATCCAGTTCG ATGTAACCCA CTCGTGCACCCAGCGTTTCT GGGTGAGCAA AAACAGGAAGGACACGGAAA TGTTGAATAC TCATACTCTTGGGTTATTGT CTCATGAGCG GATACATATTGGTTCCGCGC ACATTTCCCC GAAAAGTGCCGACATTAACC TATAAAAATA GGCGTATCACCGATCTTGCT GCGTTCGGAT ATTTTCGTGGAGATCCAGCA ACTCGCGCCA GATCATCCTGAGGACGTCGG CCGAAAGAGC GACAAGCAGATCGAGGATTT TTCGGCGCTG CGCTACGTCCGCCCACTCGA CCTTCTAGCC GACCCAGACGGTCGTCAGGC TTTCCGACGT TTGGGTGGTTAGCACTCCCG AGGGGAACCC TGTGGTTGGCCTTTTCACGC CCTTTTAAAT ATCCGTTATTCCGCCAATAT ATCCTGTCAA ACACTGATAGATCATGAGCG GAGAATTAAG GGAGTCACGTCGTTTTACGT TTGGAACTGA CAGAACCGCACGATTGTAAT ACGACTCACT ATAGGGCGAAAAGAGCGGTT ACCAGAGCTG GTCACCTTTGAATTAAGTCA GGCGCGCCTC TAGTTGAAGATCAGTAGTCT TCGGCCAGAA TGGCCCGGACTCGATGTGTA GTCTACGAGA AGGGTTAACCAATAAGCCGA TGAGGATAAA TAAAATGTGGAAGAGGATGC TTTTCCGATG AGCTCTAGTAGTGAAATATT TTGTGCTCAT TTAGTGATGGGACATTTCAG TTTTGCCACT CTTAGGTTTTAAAACAATTT TATTTTACTT TTACCACTCTCTAGAAATTC TGTTTATGCC ACAGAATGTGGTGTTCATGG CATGGAAATG TGACATAAAGCCTCGTAACA AGAGACGGAA ACATCATGAGTTTGGATGAT GCGCATGAAT GGAGTCGTCTGTCCGGGCGG CAACTACCAT CGGCGAACGATGCGCTACCT TCGTCAGCGA CGATGGCCGCGCGGCACATG GCGAGCTCAG ACCGTGCGTGCCTAGCTAGA AACTTACACC TGCAACTGCGTCCCCCGGTC GCCACCATGG CCTCCTCCGAGGTGCGCATG GAGGGCACCG TGAACGGCCA

TGGAACGAAA ACTCACGTTA AGGGATTTTG 7080TAGATCCTTT TAAATTAAAA ATGAAGTTTT 7140TGGTCTGACA GTTACCAATG CTTAATCAGT 7200CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 72 60CCATCTGGCC CCAGTGCTGC AATGATACCG 7 320TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 7 380GCCTCCATCC AGTCTATTAA TTGTTGCCGG 74 40AGTTTGCGCA ACGTTGTTGC CATTGCTGCA 7500TCATTCCACG GACAAAAACA GAGAAAGGAA 7560CACCTGTCGT TTCCTTTCTT TTCAGAGGGT 7 620AGAAGAACGG AAACGCCTTA AACCGGAAAA 7 680GCCGCCCCGT AACCTGTCGG ATCACCGGAA 774 0TACATATCAC AACGTGCGTG GAGGCCATCA 7 800GTATCGTATT AATTGATCTG CATCAACTTA 78 60AGCGACACTG AATACGGGGC AACCTCATGT 7 920GGTGTCACGC TCGTCGTTTG GTATGGCTTC 7980AGTTACATGA TCCCCCATGT TGTGCAAAAA 8040TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 8100TCTTACTGTC ATGCCATCCG TAAGATGCTT 8160ATTCTGAGAA TAGTGTATGC GGCGACCGAG 8220TACCGCGCCA CATAGCAGAA CTTTAAAAGT 8280AAAACTCTCA AGGATCTTAC CGCTGTTGAG 834 0CAACTGATCT TCAGCATCTT TTACTTTCAC 84 00GCAAAATGCC GCAAAAAAGG GAATAAGGGC 84 60CCTTTTTCAA TATTATTGAA GCATTTATCA 8520TGAATGTATT TAGAAAAATA AACAAATAGG 8580ACCTGACGTC TAAGAAACCA TTATTATCAT 8 64 0GAGGCCCTTT CGTCTTCAAG AATTGGTCGA 8700AGTTCCCGCC ACAGACCCGG ATTGAAGGCG 87 60TGACGGAACT TTGGCGCGTG ATGACTGGCC 8820TCACGCTTTT CGACAGCGTC GGATTTGCGA 8880GCGACCGCGT TGAGGGATCA AGCCACAGCA 894 0AGCCAAGGGA TCTTTTTGGA ATGCTGCTCC 9000GAACAGAAGT CATTATCGTA CGGAATGCCA 9060ATGCACATAC AAATGGACGA ACGGATAAAC 9120CTAATAAACG CTCTTTTCTC TTAGGTTTAC 9180TTTAAACTGA AGGCGGGAAA CGACAATCTG 924 0TATGACCCCC GCCGATGACG CGGGACAAGC 9300ACGTTGAAGG AGCCACTCAG CAAGCTGGTA 9360TTGAGCGCTG TTTAAACGCT CTTCAACTGG 9420TCCACCAAGA TGGAACTGCG GCCGCTCATT 94 80CACGTTCATG TCTTCATCGT AAGAAGACAC 954 0CGAAGCTGGC CGCTCTAGAA CTAGTGGATC 9600GTCTCTTCGT GAGAATAACC GTGGCCTAAA 9660TGGTACAGTA CTTCAAGAGG TTTACTCATC 9720GTACATCGGA CCTCACATAC CTCCATTGTG 97 80GTAAATTTTG TTTATGTCAC TCTAGGTTTT 984 0GACAAATAAT TTCCATTCCG CGGCAAAAGC 9900TAGCTTTCAC AATGTATCAC AAATGCCACT 9960AAAAAAAACA CTCACTTATT TGAAGCCAAG 10020TAACGTTCGT GTATAAGAAA AAATTGTACT 10080ACAATCGCGT TTGGAAGGCT TTGCATCACC 10140GCTTGCTAGC CTTCGCCTAC CGCCCACTGA 10200CCCAGCTGAC CTCTACCGAC CGGACTTGAA 10260GTACGCTGGC GACGTGCCCC CGCATGCATG 10320GCTGGCTACA AATACGTACC CCGTGAGTGC 10380AGAGCGAGCG TGTGAGTGTA GCCGAGTAGA 10440GAACGTCATC ACCGAGTTCA TGCGCTTCAA 10500CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG 10560CCGCCCCTAC GAGGGCCACA ACACCGTGAA GCTGAAGGTG ACCAAGGGCG GCCCCCTGCC 10620CTTCGCCTGG GACATCCTGT CCCCCCAGTT CCAGTACGGC TCCAAGGTGT ACGTGAAGCA 10680CCCCGCCGAC ATCCCCGACT ACAAGAAGCT GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG 10740CGTGATGAAC TTCGAGGACG GCGGCGTGGC GACCGTGACC CAGGACTCCT CCCTGCAGGA 10800CGGCTGCTTC ATCTACAAGG TGAAGTTCAT CGGCGTGAAC TTCCCCTCCG ACGGCCCCGT 108 60GATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCACCGAG CGCCTGTACC CCCGCGACGG 10920CGTGCTGAAG GGCGAGACCC ACAAGGCCCT GAAGCTGAAG GACGGCGGCC ACTACCTGGT 10980GGAGTTCAAG TCCATCTACA TGGCCAAGAA GCCCGTGCAG CTGCCCGGCT ACTACTACGT 1104 0GGACGCCAAG CTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AGCAGTACGA 11100GCGCACCGAG GGCCGCCACC ACCTGTTCCT GTAGCGGCCC ATGGATATTC GAACGCGTAG 11160GTACCACATG GTTAACCTAG ACTTGTCCAT CTTCTGGATT GGCCAACTTA ATTAATGTAT 11220GAAATAAAAG GATGCACACA TAGTGACATG CTAATCACTA TAATGTGGGC ATCAAAGTTG 11280TGTGTTATGT GTAATTACTA GTTATCTGAA TAAAAGAGAA AGAGATCATC CATATTTCTT 1134 0ATCCTAAATG AATGTCACGT GTCTTTATAA TTCTTTGATG AACCAGATGC ATTTCATTAA 114 00CCAAATCCAT ATACATATAA ATATTAATCA TATATAATTA ATATCAATTG GGTTAGCAAA 114 60ACAAATCTAG TCTAGGTGTG TTTTGCGAAT GCGGCCGCCA CCGCGGTGGA GCTCGAATTC 11520CGGTCCGGGC CTAGAAGGCC ATTTAAATCC TGAGGATCTG GTCTTCCTAA GGACCCGGGA 11580TATCGCTATC AACTTTGTAT AGAAAAGTTG GGCCGAATTC GAGCTCGGTA CGGCCAGAAT 11640GGCCCGGACC GGGTTACCGA ATTCGAGCTC GGTACCCTGG GATCCGCAAG GGACGACCGT 117 00GGTCCTTGTT TGATTTACTT CCAGGATTAT ATAATCCAGC TTATGGATTA TATAAGTACC 117 60TATTGACGTC ACGTGCTTAT GTATTATAAT AATCTAGGTA TATAGATTAT ATAATCTATC 11820TAATAATAAT CTGTGTTGTT TGTTTATCTC TCAAAACAAA CAGGTCCTAA AATGGTCCCG 11880GGCGTCCAAT GTGTCGTCAA GTAGTGTTAA GCTAAATCGA CATTTCTTTG TGGGTTGTGT 11940GGAAGGTGTT CCTTTTCCTT AAGTTGTTAG TTGTGCAAGG TGTTCCTTAG AGCATCTCCA 12000ATAGGACCTA TAATGGATTC TATTTTGAAT TATAAGACTC TAACAACAAA AGCATACTTT 12060AATGGGGATT CTATTTTACA AAAAAATATC AAATGATTAT ATGGTCGATT CCTCGGGTCC 12120TAAATATAGT ATCTCATATA ATAGAGCTCT ATCCTCATTT TATATACTAT TTTTAAGTTT 12180TTATTTACTA AATAACATGA TTTATTTTCT AATACTATGA ACTCAACTAT TAGAGCTGTA 1224 0AACGTTTTTG TGGTACTAAA CACTTTAAAT CAGGTCCTAT TTTAATTTGA AGGACTTAAA 12300TATAAGACTT CTGGTTAGAG ATGCTCTTAG CGAGTGTTTG TGCATGATTG CTATTTAGTC 12360TTTGTGGATT GTGGAAGGTG TTACTTTTCC TCAAGTTGTT AGTTGTGCAA GGTGTTTCTT 12420AGAGCATCTC TAACAGGAGC CTTAACGGAA TCTATTTTGA AGTATAGTAC TTTAACACCA 124 80AAAACATACT TTAATAGGGG TCCTATTTTA CAAAAAAATT ATCAAATGAT TATAAGGTCC 12540ACTCCTCGGG TCCTAAATAT AATATCTCAT ATACTAGAGC TCTATCCTCA TTTTATATAC 12600TATCCCTAGG TTTTTATTCC CTAAATAACA TGATTTATTT CCTAATACTA AGATATAGGG 12 660CTCAACTATT GGAGTTGCAA ATGTTTTTTG GCACTAAACA CTTTATATCA GGTCCTATTT 12720TAATTTTAAT TTGAAGGACT CAAATATAGG ACTTCTCGTT AGAGATGCTC TTAGCGAGTG 12780TTTGTGCATG ATTGCTATTT ATGTCTGTAG TTTAGTTGGG GGCTTTAATA TGTTTAGTTG 1284 0AAGTTCTAGT ATTTTTTAGG TTCTCCACTC TTTGGATTAT GACAACGACC ACTATCCAAG 12 900CAGTCTTTGA GTGCAAACGC GCGAGCAAAC TATCTGATCT ATTAAATTAT GATCCAACCG 12960TTATGTCATA TTGAAGACTT AAACCCTTTC ACCACCAGCC CAAGTATCTT TATGAAAAAC 13020CCTAACAAAC CACAATTGCA TCTATGGTTG GATTATAATT TAACGTATCA GATGGTTCGC 13080TTGCATGCTT ACATATCTAG AAACTGTTTG CATAACAGTC GTTCTCTTTG GTTATATAAT 1314 0GCTTTAGTAA TCATCAGCCA AGTGTAAACA AATGGTACAA ACTAGTAGTG AACACATCCT 13200CCCTACCTAT CTCTAGGGGT GTAACTAGAT ATCCGAATTC TTAGAACAAA TTTCATATTT 13260TAAAATAGAT ATGCTTCAAA ATTTATGCTA ATCTTTTTTA TATTATCAAG CATATTATTA 13320CACATAAGAA TAAAATTTTG TATAGAATTT TATCCATTAT TTGTTCCCTA GAATTTAAAA 13380AGTGAAAAAA CATTCGAATC TGTATCAGTT TCGTATTCAA ATTTTTACAT CTATTATTTG 13440AGAATATATA TGATAAATTT GAGGTTTAGT TTTTATGAAT CTTTACAAGG TTAATGTTAA 13500ATACATGACT ATGGATTTAC ATAGTAAATT CTATGTCTTA TTTGTCCGCG ATTGAAGAAA 13560AATGACAAAA AGATCTGACA TTCGAATAAA CATCTGTTTC CACTCCTACC TATCTGACCT 13620CCTATTTCAA ACTCCACTTT GTAACACGGT ACAAAATCAC TCCCTACCTA TCTGACCTCC 13680TATTTCAAAC TCCACTCAGT AAACAATATT GTCTATGGTA CAAAACCAAG TGTTTTATAC 13740ATCTATTTGC ACGATCTGCT CGAGTCAGGC ATCCTTGACA CACAACATAC TCCTTGTGGC 13800TATAAATGTC CAAATAGAGC AGACCTAATG GGTGGACCGT TGCATGACAC GACTTATCCC 138 60AAGACGAGCA CAGTTCGCCC CATTGGTCAT GGGGGTCCGG GCTAGTCTAG CCTGATCATC 13920GGGTCACACT TAGGCCACAG GTGTGCCACA ACGGGATAGC CCAACATGTC CCTTTTTGTC 13980ATGCATATAT CTATATTATA GTTAGTATAA TGTAAAAAAA CAAAAGGTAT GTGTGTTATG 14 040TTGGTTAGAT GTGTTTAAAT AACTCTTTAA AGCTAGCAAC TATGGTTTAA ATCATACATA 14100TACACATTTT TATTTTATTT TTATTTAAAC GATATGGGCC TTCTAGGCAC GTCGAGTGTG 14160ACGGGCCAGT GAGATGACAC ATTATAATTA CTGGTCTAGC AGGCCGTACC TAGGTCTTTC 14220TCGTGGGCCA AGACTAAGGG TTGGCCCGTT GGCTAATCTG TACGGTACCG ATACTGTCCT 14280AATTCATTTG AACACCTGTA GAAGAGGGGA ATTTATAATT GAGGAGGAAT GTACTCATGC 14 340GGTACACCAG GGGAATTGTT TTGTTGTGCT CAGCGATAGA TTTCAACGCA ACGGTGAGCC 144 00AGTTTCACTA AAAAAAGGGG GGGGGGGGGG GGGGGGGGAA GGCCACATCA AAGGCGAGGT 14 4 60GCTGACGAGC AGAAGATGCT AGCAGTGACG CCAAGTCCAG CAGCTAGCAA TGAAAGGGTA 14 520CTCGGGATTT AACAATGCCT AGAGACGGCA TCATCCCCTC AATAATCCGG TGCTCTCTTT 14 580TTGTTTATTC ACCAGTTGGC GTAGCTATAT ACACATGTCT GGTCTGACGA ACAAATCAAG 14 640GGATCGCTAG CTCGGGCTAG CCTTCCTATC ACTGTCATGA CATGTGCTCT GCCTCTGCTG 14700GTTGATAAGC CGTGCGCCTT CTCGCTAATT CTTTCTTGTG CTAGAGGCGA GTCAAACAAA 14 760CGCTGCACCT CGTAGCCCTT AATCTGCGCT AAGGGTCACA TGACCCTGTT CCCTATCGCT 14820AGTTACCAAC GACCCATTCC CCCTGACAGA TACTTACGAC GCGTCCGTAC GCGGCAGGCC 14 880TCGGCAGTTC GGCATCACCA GCACCGGCGC CGGCATTCGC CCCCTGCCAG CCGGTTCGCA 14 940GATTCGCAGG GCGGAGTCGG CCGCAGTTGC CGCATCCCAA ACGCCCGGGA ACCTTTGGGG 15000CCCCTCTACG AGCAAATGAA GTTGCTGCCC CTGGCTTCGT AAAGCTCTGA CTTTTGATCA 15060CTTGATTGGC AGTCGTACTC CTCGCTCATA GGCCGACACG GCCGCAAAGT CAACTACCCG 15120CTCCGCCATC CTTCAACCCC CGCCACGCGC CTATATATGT TCGCGGCCAT GTCCGTACTA 15180GTCCTCCAAC CCACAAGCCA CAACCCCGAG CTCAGATCCC TCGCCTCGTG TCGTGTCTCC 15240GGTCGACGAC GACCAACAGC CAGTGTGGGC CAGACGGACA CCGCCGAGCT ATAGCGCTTG 15300GTGATAAAGC TTGGTCACCC GGTCCGGGCC TAGAAGGCCA GCTTCAAGTT TGTACAAAAA 15360AGCAGGCTCC AGCGCTCACC ATGGTCCGTC CTGTAGAAAC CCCAACCCGT GAAATCAAAA 15420AACTCGACGG CCTGTGGGCA TTCAGTCTGG ATCGCGAAAA CTGTGGAATT GATCAGCGTT 15480GGTGGGAAAG CGCGTTACAA GAAAGCCGGG CAATTGCTGT GCCAGGCAGT TTTAACGATC 1554 0AGTTCGCCGA TGCAGATATT CGTAATTATG CGGGCAACGT CTGGTATCAG CGCGAAGTCT 15600TTATACCGAA AGGTTGGGCA GGCCAGCGTA TCGTGCTGCG TTTCGATGCG GTCACTCATT 15660ACGGCAAAGT GTGGGTCAAT AATCAGGAAG TGATGGAGCA TCAGGGCGGC TATACGCCAT 157 20TTGAAGCCGA TGTCACGCCG TATGTTATTG CCGGGAAAAG TGTACGTAAG TTTCTGCTTC 157 80TACCTTTGAT ATATATATAA TAATTATCAT TAATTAGTAG TAATATAATA TTTCAAATAT 158 4 0TTTTTTCAAA ATAAAAGAAT GTAGTATATA GCAATTGCTT TTCTGTAGTT TATAAGTGTG 15900TATATTTTAA TTTATAACTT TTCTAATATA TGACCAAAAT TTGTTGATGT GCAGGTATCA 15960CCGTTTGTGT GAACAACGAA CTGAACTGGC AGACTATCCC GCCGGGAATG GTGATTACCG 16020ACGAAAACGG CAAGAAAAAG CAGTCTTACT TCCATGATTT CTTTAACTAT GCCGGAATCC 16080ATCGCAGCGT AATGCTCTAC ACCACGCCGA ACACCTGGGT GGACGATATC ACCGTGGTGA 1614 0CGCATGTCGC GCAAGACTGT AACCACGCGT CTGTTGACTG GCAGGTGGTG GCCAATGGTG 16200ATGTCAGCGT TGAACTGCGT GATGCGGATC AACAGGTGGT TGCAACTGGA CAAGGCACTA 16260GCGGGACTTT GCAAGTGGTG AATCCGCACC TCTGGCAACC GGGTGAAGGT TATCTCTATG 16320AACTGTGCGT CACAGCCAAA AGCCAGACAG AGTGTGATAT CTACCCGCTT CGCGTCGGCA 16380TCCGGTCAGT GGCAGTGAAG GGCGAACAGT TCCTGATTAA CCACAAACCG TTCTACTTTA 16440CTGGCTTTGG TCGTCATGAA GATGCGGACT TGCGTGGCAA AGGATTCGAT AACGTGCTGA 16500TGGTGCACGA CCACGCATTA ATGGACTGGA TTGGGGCCAA CTCCTACCGT ACCTCGCATT 16560ACCCTTACGC TGAAGAGATG CTCGACTGGG CAGATGAACA TGGCATCGTG GTGATTGATG 16620AAACTGCTGC TGTCGGCTTT AACCTCTCTT TAGGCATTGG TTTCGAAGCG GGCAACAAGC 16680CGAAAGAACT GTACAGCGAA GAGGCAGTCA ACGGGGAAAC TCAGCAAGCG CACTTACAGG 16740CGATTAAAGA GCTGATAGCG CGTGACAAAA ACCACCCAAG CGTGGTGATG TGGAGTATTG 16800CCAACGAACC GGATACCCGT CCGCAAGGTG CACGGGAATA TTTCGCGCCA CTGGCGGAAG 168 60CAACGCGTAA ACTCGACCCG ACGCGTCCGA TCACCTGCGT CAATGTAATG TTCTGCGACG 16920CTCACACCGA TACCATCAGC GATCTCTTTG ATGTGCTGTG CCTGAACCGT TATTACGGAT 16980GGTATGTCCA AAGCGGCGAT TTGGAAACGG CAGAGAAGGT ACTGGAAAAA GAACTTCTGG 17040CCTGGCAGGA GAAACTGCAT CAGCCGATTA TCATCACCGA ATACGGCGTG GATACGTTAG 17100CCGGGCTGCA CTCAATGTAC ACCGACATGT GGAGTGAAGA GTATCAGTGT GCATGGCTGG 17160ATATGTATCA CCGCGTCTTT GATCGCGTCA GCGCCGTCGT CGGTGAACAG GTATGGAATT 17220TCGCCGATTT TGCGACCTCG CAAGGCATAT TGCGCGTTGG CGGTAACAAG AAAGGGATCT 17280TCACTCGCGA CCGCAAACCG AAGTCGGCGG CTTTTCTGCT GCAAAAACGC TGGACTGGCA 1734 0TGAACTTCGG TGAAAAACCG CAGCAGGGAG GCAAACAATG AAGATCTCCC GGGCACCCAG 17 4 00CTTTCTTGTA CAAAGTGGCC GTTAACGGAT CCAGACTTGT CCATCTTCTG GATTGGCCAA 174 60CTTAATTAAT GTATGAAATA AAAGGATGCA CACATAGTGA CATGCTAATC ACTATAATGT 17520GGGCATCAAA GTTGTGTGTT ATGTGTAATT ACTAGTTATC TGAATAAAAG AGAAAGAGAT 17580CATCCATATT TCTTATCCTA AATGAATGTC ACGTGTCTTT ATAATTCTTT GATGAACCAG 17640ATGCATTTCA TTAACCAAAT CCATATACAT ATAAATATTA ATCATATATA ATTAATATCA 17700

ATTGGGTTAG CAAAACAAAT CTAGTCTAGG TGTGTTTTGC GAATTGCGGC AAGCTTGCGG 177 60

CCGCCCCGGG CAACTTT 177 77

<210> 88<211> 54686<212> DNA<213> VETOR

<400> 88

TCTAGAGCTC GTTCCTCGAG GCCTCGAGGC CTCGAGGAAC GGTACCTGCG GGGAAGCTTA 60

CAATAATGTG TGTTGTTAAG TCTTGTTGCC TGTCATCGTC TGACTGACTT TCGTCATAAA 120

TCCCGGCCTC CGTAACCCAG CTTTGGGCAA GCTCACGGAT TTGATCCGGC GGAACGGGAA 180

TATCGAGATG CCGGGCTGAA CGCTGCAGTT CCAGCTTTCC CTTTCGGGAC AGGTACTCCA 24 0

GCTGATTGAT TATCTGCTGA AGGGTCTTGG TTCCACCTCC TGGCACAATG CGAATGATTA 300

CTTGAGCGCG ATCGGGCATC CAATTTTCTC CCGTCAGGTG CGTGGTCAAG TGCTACAAGG 360

CACCTTTCAG TAACGAGCGA CCGTCGATCC GTCGCCGGGA TACGGACAAA ATGGAGCGCA 4 20

GTAGTCCATC GAGGGCGGCG AAAGCCTCGC CAAAAGCAAT ACGTTCATCT CGCACAGCCT 4 80

CCAGATCCGA TCGAGGGTCT TCGGCGTAGG CAGATAGAAG CATGGATACA TTGCTTGAGA 54 0

GTATTCCGAT GGACTGAAGT ATGGCTTCCA TCTTTTCTCG TGTGTCTGCA TCTATTTCGA 600

GAAAGCCCCC GATGCGGCGC ACCGCAACGC GAATTGCCAT ACTATCCGAA AGTCCCAGCA 660

GGCGCGCTTG ATAGGAAAAG GTTTCATACT CGGCCGATCG CAGACGGGCA CTCACGACCT 720

TGAACCCTTC AACTTTCAGG GATCGATGCT GGTTGATGGT AGTCTCACTC GACGTGGCTC 7 80

TGGTGTGTTT TGACATAGCT TCCTCCAAAG AAAGCGGAAG GTCTGGATAC TCCAGCACGA 840

AATGTGCCCG GGTAGACGGA TGGAAGTCTA GCCCTGCTCA ATATGAAATC AACAGTACAT 900

TTACAGTCAA TACTGAATAT ACTTGCTACA TTTGCAATTG TCTTATAACG AATGTGAAAT 960

AAAAATAGTG TAACAACGCT TTTACTCATC GATAATCACA AAAACATTTA TACGAACAAA 1020

AATACAAATG CACTCCGGTT TCACAGGATA GGCGGGATCA GAATATGCAA CTTTTGACGT 1080

TTTGTTCTTT CAAAGGGGGT GCTGGCAAAA CCACCGCACT CATGGGCCTT TGCGCTGCTT 114 0

TGGCAAATGA CGGTAAACGA GTGGCCCTCT TTGATGCCGA CGAAAACCGG CCTCTGACGC 1200

GATGGAGAGA AAACGCCTTA CAAAGCAGTA CTGGGATCCT CGCTGTGAAG TCTATTCCGC 1260

CGACGAAATG CCCCTTCTTG AAGCAGCCTA TGAAAATGCC GAGCTCGAAG GATTTGATTA 1320

TGCGTTGGCC GATACGCGTG GCGGCTCGAG CGAGCTCAAC AACACAATCA TCGCTAGCTC 1380

AAACCTGCTT CTGATCCCCA CCATGCTAAC GCCGCTCGAC ATCGATGAGG CACTATCTAC 14 40

CTACCGCTAC GTCATCGAGC TGCTGTTGAG TGAAAATTTG GCAATTCCTA CAGCTGTTTT 1500

GCGCCAACGC GTCCCGGTCG GCCGATTGAC AACATCGCAA CGCAGGATGT CAGAGACGCT 1560

AGAGAGCCTT CCAGTTGTAC CGTCTCCCAT GCATGAAAGA GATGCATTTG CCGCGATGAA 1620

AGAACGCGGC ATGTTGCATC TTACATTACT AAACACGGGA ACTGATCCGA CGATGCGCCT 1680

CATAGAGAGG AATCTTCGGA TTGCGATGGA GGAAGTCGTG GTCATTTCGA AACTGATCAG 1740

CAAAATCTTG GAGGCTTGAA GATGGCAATT CGCAAGCCCG CATTGTCGGT CGGCGAAGCA 1800

CGGCGGCTTG CTGGTGCTCG ACCCGAGATC CACCATCCCA ACCCGACACT TGTTCCCCAG 18 60

AAGCTGGACC TCCAGCACTT GCCTGAAAAA GCCGACGAGA AAGACCAGCA ACGTGAGCCT 1920

CTCGTCGCCG ATCACATTTA CAGTCCCGAT CGACAACTTA AGCTAACTGT GGATGCCCTT 1980

AGTCCACCTC CGTCCCCGAA AAAGCTCCAG GTTTTTCTTT CAGCGCGACC GCCCGCGCCT 2040

CAAGTGTCGA AAACATATGA CAACCTCGTT CGGCAATACA GTCCCTCGAA GTCGCTACAA 2100

ATGATTTTAA GGCGCGCGTT GGACGATTTC GAAAGCATGC TGGCAGATGG ATCATTTCGC 2160

GTGGCCCCGA AAAGTTATCC GATCCCTTCA ACTACAGAAA AATCCGTTCT CGTTCAGACC 2220

TCACGCATGT TCCCGGTTGC GTTGCTCGAG GTCGCTCGAA GTCATTTTGA TCCGTTGGGG 2280

TTGGAGACCG CTCGAGCTTT CGGCCACAAG CTGGCTACCG CCGCGCTCGC GTCATTCTTT 2340

GCTGGAGAGA AGCCATCGAG CAATTGGTGA AGAGGGACCT ATCGGAACCC CTCACCAAAT 2400

ATTGAGTGTA GGTTTGAGGC CGCTGGCCGC GTCCTCAGTC ACCTTTTGAG CCAGATAATT 24 60

AAGAGCCAAA TGCAATTGGC TCAGGCTGCC ATCGTCCCCC CGTGCGAAAC CTGCACGTCC 2520

GCGTCAAAGA AATAACCGGC ACCTCTTGCT GTTTTTATCA GTTGAGGGCT TGACGGATCC 2580

GCCTCAAGTT TGCGGCGCAG CCGCAAAATG AGAACATCTA TACTCCTGTC GTAAACCTCC 2 64 0

TCGTCGCGTA CTCGACTGGC AATGAGAAGT TGCTCGCGCG ATAGAACGTC GCGGGGTTTC 2700

TCTAAAAACG CGAGGAGAAG ATTGAACTCA CCTGCCGTAA GTTTCACCTC ACCGCCAGCT 27 60

TCGGACATCA AGCGACGTTG CCTGAGATTA AGTGTCCAGT CAGTAAAACA AAAAGACCGT 2820

CGGTCTTTGG AGCGGACAAC GTTGGGGCGC ACGCGCAAGG CAACCCGAAT GCGTGCAAGA 2880

AACTCTCTCG TACTAAACGG CTTAGCGATA AAATCACTTG CTCCTAGCTC GAGTGCAACA 294 0ACTTTATCCG TCTCCTCAAG GCGGTCGCCA CTGATAATTA TGATTGGAAT ATCAGACTTT 3000

GCCGCCAGAT TTCGAACGAT CTCAAGCCCA TCTTCACGAC CTAAATTTAG ATCAACAACC 3060

ACGACATCGA CCGTCGCGGA AGAGAGTACT CTAGTGAACT GGGTGCTGTC GGCTACCGCG 3120

GTCACTTTGA AGGCGTGGAT CGTAAGGTAT TCGATAATAA GATGCCGCAT AGCGACATCG 3180

TCATCGATAA GAAGAACGTG TTTCAACGGC TCACCTTTCA ATCTAAAATC TGAACCCTTG 3240

TTCACAGCGC TTGAGAAATT TTCACGTGAA GGATGTACAA TCATCTCCAG CTAAATGGGC 3300

AGTTCGTCAG AATTGCGGCT GACCGCGGAT GACGAAAATG CGAACCAAGT ATTTCAATTT 3360

TATGACAAAA GTTCTCAATC GTTGTTACAA GTGAAACGCT TCGAGGTTAC AGCTACTATT 34 20

GATTAAGGAG ATCGCCTATG GTCTCGCCCC GGCGTCGTGC GTCCGCCGCG AGCCAGATCT 3480

CGCCTACTTC ATAAACGTCC TCATAGGCAC GGAATGGAAT GATGACATCG ATCGCCGTAG 354 0

AGAGCATGTC AATCAGTGTG CGATCTTCCA AGCTAGCACC TTGGGCGCTA CTTTTGACAA 3600

GGGAAAACAG TTTCTTGAAT CCTTGGATTG GATTCGCGCC GTGTATTGTT GAAATCGATC 3660

CCGGATGTCC CGAGACGACT TCACTCAGAT AAGCCCATGC TGCATCGTCG CGCATCTCGC 3720

CAAGCAATAT CCGGTCCGGC CGCATACGCA GACTTGCTTG GAGCAAGTGC TCGGCGCTCA 3780

CAGCACCCAG CCCAGCACCG TTCTTGGAGT AGAGTAGTCT AACATGATTA TCGTGTGGAA 384 0

TGACGAGTTC GAGCGTATCT TCTATGGTGA TTAGCCTTTC CTGGGGGGGG ATGGCGCTGA 3900

TCAAGGTCTT GCTCATTGTT GTCTTGCCGC TTCCGGTAGG GCCACATAGC AACATCGTCA 3960

GTCGGCTGAC GACGCATGCG TGCAGAAACG CTTCCAAATC CCCGTTGTCA AAATGCTGAA 4 020

GGATAGCTTC ATCATCCTGA TTTTGGCGTT TCCTTCGTGT CTGCCACTGG TTCCACCTCG 4 080

AAGCATCATA ACGGGAGGAG ACTTCTTTAA GACCAGAAAC ACGCGAGCTT GGCCGTCGAA 414 0

TGGTCAAGCT GACGGTGCCC GAGGGAACGG TCGGCGGCAG ACAGATTTGT AGTCGTTCAC 4 200

CACCAGGAAG TTCAGTGGCG CAGAGGGGGT TACGTGGTCC GACATCCTGC TTTCTCAGCG 4 2 60

CGCCCGCTAA AATAGCGATA TCTTCAAGAT CATCATAAGA GACGGGCAAA GGCATCTTGG 4 320

TAAAAATGCC GGCTTGGCGC ACAAATGCCT CTCCAGGTCG ATTGATCGCA ATTTCTTCAG 4 380

TCTTCGGGTC ATCGAGCCAT TCCAAAATCG GCTTCAGAAG AAAGCGTAGT TGCGGATCCA 4 4 40

CTTCCATTTA CAATGTATCC TATCTCTAAG CGGAAATTTG AATTCATTAA GAGCGGCGGT 4 500

TCCTCCCCCG CGTGGCGCCG CCAGTCAGGC GGAGCTGGTA AACACCAAAG AAATCGAGGT 4 560

CCCGTGCTAC GAAAATGGAA ACGGTGTCAC CCTGATTCTT CTTCAGGGTT GGCGGTATGT 4 620

TGATGGTTGC CTTAAGGGCT GTCTCAGTTG TCTGCTCACC GTTATTTTGA AAGCTGTTGA 4 680

AGCTCATCCC GCCACCCGAG CTGCCGGCGT AGGTGCTAGC TGCCTGGAAG GCGCCTTGAA 4740

CAACACTCAA GAGCATAGCT CCGCTAAAAC GCTGCCAGAA GTGGCTGTCG ACCGAGCCCG 4 800

GCAATCCTGA GCGACCGAGT TCGTCCGCGC TTGGCGATGT TAACGAGATC ATCGCATGGT 4 8 60

CAGGTGTCTC GGCGCGATCC CACAACACAA AAACGCGCCC ATCTCCCTGT TGCAAGCCAC 4 920

GCTGTATTTC GCCAACAACG GTGGTGCCAC GATCAAGAAG CACGATATTG TTCGTTGTTC 4 980

CACGAATATC CTGAGGCAAG ACACACTTTA CATAGCCTGC CAAATTTGTG TCGATTGCGG 5040

TTTGCAAGAT GCACGGAATT ATTGTCCCTT GCGTTACCAT AAAATCGGGG TGCGGCAAGA 5100

GCGTGGCGCT GCTGGGCTGC AGCTCGGTGG GTTTCATACG TATCGACAAA TCGTTCTCGC 5160

CGGACACTTC GCCATTCGGC AAGGAGTTGT CGTCACGCTT GCCTTCTTGT CTTCGGCCCG 5220

TGTCGCCCTG AATGGCGCGT TTGCTGACCC CTTGATCGCC GCTGCTATAT GCAAAAATCG 5280

GTGTTTCTTC CGGCCGTGGC TCATGCCGCT CCGGTTCGCC CCTCGGCGGT AGAGGAGCAG 534 0

CAGGCTGAAC AGCCTCTTGA ACCGCTGGAG GATCCGGCGG CACCTCAATC GGAGCTGGAT 54 00

GAAATGGCTT GGTGTTTGTT GCGATCAAAG TTGACGGCGA TGCGTTCTCA TTCACCTTCT 54 60

TTTGGCGCCC ACCTAGCCAA ATGAGGCTTA ATGATAACGC GAGAACGACA CCTCCGACGA 5520

TCAATTTCTG AGACCCCGAA AGACGCCGGC GATGTTTGTC GGAGACCAGG GATCCAGATG 5580

CATCAACCTC ATGTGCCGCT TGCTGACTAT CGTTATTCAT CCCTTCGCCC CCTTCAGGAC 564 0

GCGTTTCACA TCGGGCCTCA CCGTGCCCGT TTGCGGCCTT TGGCCAACGG GATCGTAAGC 57 00

GGTGTTCCAG ATACATAGTA CTGTGTGGCC ATCCCTCAGA CGCCAACCTC GGGAAACCGA 57 60

AGAAATCTCG ACATCGCTCC CTTTAACTGA ATAGTTGGCA ACAGCTTCCT TGCCATCAGG 5820

ATTGATGGTG TAGATGGAGG GTATGCGTAC ATTGCCCGGA AAGTGGAATA CCGTCGTAAA 5880

TCCATTGTCG AAGACTTCGA GTGGCAACAG CGAACGATCG CCTTGGGCGA CGTAGTGCCA 5940

ATTACTGTCC GCCGCACCAA GGGCTGTGAC AGGCTGATCC AATAAATTCT CAGCTTTCCG 6000

TTGATATTGT GCTTCCGCGT GTAGTCTGTC CACAACAGCC TTCTGTTGTG CCTCCCTTCG 6060

CCGAGCCGCC GCATCGTCGG CGGGGTAGGC GAATTGGACG CTGTAATAGA GATCGGGCTG 6120

CTCTTTATCG AGGTGGGACA GAGTCTTGGA ACTTATACTG AAAACATAAC GGCGCATCCC 6180

GGAGTCGCTT GCGGTTAGCA CGATTACTGG CTGAGGCGTG AGGACCTGGC TTGCCTTGAA 624 0

AAATAGATAA TTTCCCCGCG GTAGGGCTGC TAGATCTTTG CTATTTGAAA CGGCAACCGC 6300

TGTCACCGTT TCGTTCGTGG CGAATGTTAC GACCAAAGTA GCTCCAACCG CCGTCGAGAG 6360

GCGCACCACT TGATCGGGAT TGTAAGCCAA ATAACGCATG CGCGGATCTA GCTTGCCCGC 6420

CATTGGAGTG TCTTCAGCCT CCGCACCAGT CGCAGCGGCA AATAAACATG CTAAAATGAA 64 80AAGTGCTTTT CTGATCATGG TTCGCTGTGG CCTACGTTTG AAACGGTATC TTCCGATGTC 6540

TGATAGGAGG TGACAACCAG ACCTGCCGGG TTGGTTAGTC TCAATCTGCC GGGCAAGCTG 6600

GTCACCTTTT CGTAGCGAAC TGTCGCGGTC CACGTACTCA CCACAGGCAT TTTGCCGTCA 6660

ACGACGAGGG TCCTTTTATA GCGAATTTGC TGCGTGCTTG GAGTTACATC ATTTGAAGCG 6720

ATGTGCTCGA CCTCCACCCT GCCGCGTTTG CCAAGAATGA CTTGAGGCGA ACTGGGATTG 6780

GGATAGTTGA AGAATTGCTG GTAATCCTGG CGCACTGTTG GGGCACTGAA GTTCGATACC 6840

AGGTCGTAGG CGTACTGAGC GGTGTCGGCA TCATAACTCT CGCGCAGGCG AACGTACTCC 6900

CACAATGAGG CGTTAACGAC GGCCTCCTCT TGAGTTGCAG GCAATCGCGA GACAGACACC 6960

TCGCTGTCAA CGGTGCCGTC CGGCCGTATC CATAGATATA CGGGCACAAG CCTGCTCAAC 7020

GGCACCATTG TGGCTATAGC GAACGCTTGA GCAACATTTC CCAAAATCGC GATAGCTGCG 7080

ACAGCTGCAA TGAGTTTGGA GAGACGTCGC GCCGATTTCG CTCGCGCGGT TTGAAAGGCT 7140

TCTACTTCCT TATAGTGCTC GGCAAGGCTT TCGCGCGCCA CTAGCATGGC ATATTCAGGC 7200

CCCGTCATAG CGTCCACCCG AATTGCCGAG CTGAAGATCT GACGGAGTAG GCTGCCATCG 7260

CCCCACATTC AGCGGGAAGA TCGGGCCTTT GCAGCTCGCT AATGTGTCGT TTGTCTGGCA 7320

GCCGCTCAAA GCGACAACTA GGCACAGCAG GCAATACTTC ATAGAATTCT CCATTGAGGC 7 380

GAATTTTTGC GCGACCTAGC CTCGCTCAAC CTGAGCGAAG CGACGGTACA AGCTGCTGGC 7440

AGATTGGGTT GCGCCGCTCC AGTAACTGCC TCCAATGTTG CCGGCGATCG CCGGCAAAGC 7500

GACAATGAGC GCATCCCCTG TCAGAAAAAA CATATCGAGT TCGTAAAGAC CAATGATCTT 7560

GGCCGCGGTC GTACCGGCGA AGGTGATTAC ACCAAGCATA AGGGTGAGCG CAGTCGCTTC 7 620

GGTTAGGATG ACGATCGTTG CCACGAGGTT TAAGAGGAGA AGCAAGAGAC CGTAGGTGAT 7 680

AAGTTGCCCG ATCCACTTAG CTGCGATGTC CCGCGTGCGA TCAAAAATAT ATCCGACGAG 77 4 0

GATCAGAGGC CCGATCGCGA GAAGCACTTT CGTGAGAATT CCAACGGCGT CGTAAACTCC 7 800

GAAGGCAGAC CAGAGCGTGC CGTAAAGGAC CCACTGTGCC CCTTGGAAAG CAAGGATGTC 78 60

CTGGTCGTTC ATCGGACCGA TTTCGGATGC GATTTTCTGA AAAACGGCCT GGGTCACGGC 7 920

GAACATTGTA TCCAACTGTG CCGGAACAGT CTGCAGAGGC AAGCCGGTTA CACTAAACTG 7 980

CTGAACAAAG TTTGGGACCG TCTTTTCGAA GATGGAAACC ACATAGTCTT GGTAGTTAGC 804 0

CTGCCCAACA ATTAGAGCAA CAACGATGGT GACCGTGATC ACCCGAGTGA TACCGCTACG 8100

GGTATCGACT TCGCCGCGTA TGACTAAAAT ACCCTGAACA ATAATCCAAA GAGTGACACA 8160

GGCGATCAAT GGCGCACTCA CCGCCTCCTG GATAGTCTCA AGCATCGAGT CCAAGCCTGT 8220

CGTGAAGGCT ACATCGAAGA TCGTATGAAT GGCCGTAAAC GGCGCCGGAA TCGTGAAATT 8280

CATCGATTGG ACCTGAACTT GACTGGTTTG TCGCATAATG TTGGATAAAA TGAGCTCGCA 834 0

TTCGGCGAGG ATGCGGGCGG ATGAACAAAT CGCCCAGCCT TAGGGGAGGG CACCAAAGAT 84 00

GACAGCGGTC TTTTGATGCT CCTTGCGTTG AGCGGCCGCC TCTTCCGCCT CGTGAAGGCC 84 60

GGCCTGCGCG GTAGTCATCG TTAATAGGCT TGTCGCCTGT ACATTTTGAA TCATTGCGTC 8520

ATGGATCTGC TTGAGAAGCA AACCATTGGT CACGGTTGCC TGCATGATAT TGCGAGATCG 8580

GGAAAGCTGA GCAGACGTAT CAGCATTCGC CGTCAAGCGT TTGTCCATCG TTTCCAGATT 8 64 0

GTCAGCCGCA ATGCCAGCGC TGTTTGCGGA ACCGGTGATC TGCGATCGCA ACAGGTCCGC 8700

TTCAGCATCA CTACCCACGA CTGCACGATC TGTATCGCTG GTGATCGCAC GTGCCGTGGT 87 60

CGACATTGGC ATTCGCGGCG AAAACATTTC ATTGTCTAGG TCCTTCGTCG AAGGATACTG 8820

ATTTTTCTGG TTGAGCGAAG TCAGTAGTCC AGTAACGCCG TAGGCCGACG TCAACATCGT 8880

AACCATCGCT ATAGTCTGAG TGAGATTCTC CGCAGTCGCG AGCGCAGTCG CGAGCGTCTC 8 94 0

AGCCTCCGTT GCCGGGTCGC TAACAACAAA CTGCGCCCGC GCGGGCTGAA TATATAGAAA 9000

GCTGCAGGTC AAAACTGTTG CAATAAGTTG CGTCGTCTTC ATCGTTTCCT ACCTTATCAA 9060

TCTTCTGCCT CGTGGTGACG GGCCATGAAT TCGCTGAGCC AGCCAGATGA GTTGCCTTCT 9120

TGTGCCTCGC GTAGTCGAGT TGCAAAGCGC ACCGTGTTGG CACGCCCCGA AAGCACGGCG 9180

ACATATTCAC GCATATCCCG CAGATCAAAT TCGCAGATGA CGCTTCCACT TTCTCGTTTA 924 0

AGAAGAAACT TACGGCTGCC GACCGTCATG TCTTCACGGA TCGCCTGAAA TTCCTTTTCG 9300

GTACATTTCA GTCCATCGAC ATAAGCCGAT CGATCTGCGG TTGGTGATGG ATAGAAAATC 9360

TTCGTCATAC ATTGCGCAAC CAAGCTGGCT CCTAGCGGCG ATTCCAGAAC ATGCTCTGGT 94 20

TGCTGCGTTG CCAGTATTAG CATCCCGTTG TTTTTTCGAA CGGTCAGGAG GAATTTGTCG 94 80

ACGACAGTCG AAAATTTAGG GTTTAACAAA TAGGCGCGAA ACTCATCGCA GCTCATCACA 9540

AAACGGCGGC CGTCGATCAT GGCTCCAATC CGATGCAGGA GATATGCTGC AGCGGGAGCG 9600

CATACTTCCT CGTATTCGAG AAGATGCGTC ATGTCGAAGC CGGTAATCGA CGGATCTAAC 9660

TTTACTTCGT CAACTTCGCC GTCAAATGCC CAGCCAAGCG CATGGCCCCG GCACCAGCGT 9720

TGGAGCCGCG CTCCTGCGCC TTCGGCGGGC CCATGCAACA AAAATTCACG TAACCCCGCG 9780

ATTGAACGCA TTTGTGGATC AAACGAGAGC TGACGATGGA TACCACGGAC CAGACGGCGG 984 0

TTCTCTTCCG GAGAAATCCC ACCCCGACCA TCACTCTCGA TGAGAGCCAC GATCCATTCG 9900

CGCAGAAAAT CGTGTGAGGC TGCTGTGTTT TCTAGGCCAC GCAACGGCGC CAACCCGCTG 9960

GGTGTGCCTC TGTGAAGTGC CAAATATGTT CCTCCTGTGG CGCGAACCAG CAATTCGCCA 10020CCCCGGTCCT TGTCAAAGAA CACGACCGTA CCTGCACGGT CGACCATGCT CTGTTCGAGC 10080

ATGGCTAGAA CAAACATCAT GAGCGTCGTC TTACCCCTCC CGATAGGCCC GAATATTGCC 10140GTCATGCCAA CATCGTGCTC ATGCGGGATA TAGTCGAAAG GCGTTCCGCC ATTGGTACGA 10200AATCGGGCAA TCGCGTTGCC CCAGTGGCCT GAGCTGGCGC CCTCTGGAAA GTTTTCGAAA 10260GAGACAAACC CTGCGAAATT GCGTGAAGTG ATTGCGCCAG GGCGTGTGCG CCACTTAAAA 10320

TTCCCCGGCA ATTGGGACCA ATAGGCCGCT TCCATACCAA TACCTTCTTG GACAACCACG 10380

GCACCTGCAT CCGCCATTCG TGTCCGAGCC CGCGCGCCCC TGTCCCCAAG ACTATTGAGA 10440

TCGTCTGCAT AGACGCAAAG GCTCAAATGA TGTGAGCCCA TAACGAATTC GTTGCTCGCA 10500

AGTGCGTCCT CAGCCTCGGA TAATTTGCCG ATTTGAGTCA CGGCTTTATC GCCGGAACTC 10560

AGCATCTGGC TCGATTTGAG GCTAAGTTTC GCGTGCGCTT GCGGGCGAGT CAGGAACGAA 10620

AAACTCTGCG TGAGAACAAG TGGAAAATCG AGGGATAGCA GCGCGTTGAG CATGCCCGGC 10680

CGTGTTTTTG CAGGGTATTC GCGAAACGAA TAGATGGATC CAACGTAACT GTCTTTTGGC 10740

GTTCTGATCT CGAGTCCTCG CTTGCCGCAA ATGACTCTGT CGGTATAAAT CGAAGCGCCG 10800

AGTGAGCCGC TGACGACCGG AACCGGTGTG AACCGACCAG TCATGATCAA CCGTAGCGCT 10860

TCGCCAATTT CGGTGAAGAG CACACCCTGC TTCTCGCGGA TGCCAAGACG ATGCAGGCCA 10920

TACGCTTTAA GAGAGCCAGC GACAACATGC CAAAGATCTT CCATGTTCCT GATCTGGCCC 10980

GTGAGATCGT TTTCCCTTTT TCCGCTTAGC TTGGTGAACC TCCTCTTTAC CTTCCCTAAA 11040

GCCGCCTGTG GGTAGACAAT CAACGTAAGG AAGTGTTCAT TGCGGAGGAG TTGGCCGGAG 11100

AGCACGCGCT GTTCAAAAGC TTCGTTCAGG CTAGCGGCGA AAACACTACG GAAGTGTCGC 11160

GGCGCCGATG ATGGCACGTC GGCATGACGT ACGAGGTGAG CATATATTGA CACATGATCA 11220

TCAGCGATAT TGCGCAACAG CGTGTTGAAC GCACGACAAC GCGCATTGCG CATTTCAGTT 11280

TCCTCAAGCT CGAATGCAAC GCCATCAATT CTCGCAATGG TCATGATCGA TCCGTCTTCA 11340

AGAAGGACGA TATGGTCGCT GAGGTGGCCA ATATAAGGGA GATAGATCTC ACCGGATCTT 11400

TCGGTCGTTC CACTCGCGCC GAGCATCACA CCATTCCTCT CCCTCGTGGG GGAACCCTAA 11460

TTGGATTTGG GCTAACAGTA GCGCCCCCCC AAACTGCACT ATCAATGCTT CTTCCCGCGG 11520

TCCGCAAAAA TAGCAGGACG ACGCTCGCCG CATTGTAGTC TCGCTCCACG ATGAGCCGGG 11580

CTGCAAACCA TAACGGCACG AGAACGACTT CGTAGAGCGG GTTCTGAACG ATAACGATGA 11640

CAAAGCCGGC GAACATCATG AATAACCCTG CCAATGTCAG TGGCACCCCA AGAAACAATG 11700

CGGGCCGTGT GGCTGCGAGG TAAAGGGTCG ATTCTTCCAA ACGATCAGCC ATCAACTACC 11760

GCCAGTGAGC GTTTGGCCGA GGAAGCTCGC CCCAAACATG ATAACAATGC CGCCGACGAC 11820

GCCGGCAACC AGCCCAAGCG AAGCCCGCCC GAACATCCAG GAGATCCCGA TAGCGACAAT 11880

GCCGAGAACA GCGAGTGACT GGCCGAACGG ACCAAGGATA AACGTGCATA TATTGTTAAC 11940

CATTGTGGCG GGGTCAGTGC CGCCACCCGC AGATTGCGCT GCGGCGGGTC CGGATGAGGA 12000

AATGCTCCAT GCAATTGCAC CGCACAAGCT TGGGGCGCAG CTCGATATCA CGCGCATCAT 12060

CGCATTCGAG AGCGAGAGGC GATTTAGATG TAAACGGTAT CTCTCAAAGC ATCGCATCAA 12120

TGCGCACCTC CTTAGTATAA GTCGAATAAG ACTTGATTGT CGTCTGCGGA TTTGCCGTTG 12180

TCCTGGTGTG GCGGTGGCGG AGCGATTAAA CCGCCAGCGC CATCCTCCTG CGAGCGGCGC 12240

TGATATGACC CCCAAACATC CCACGTCTCT TCGGATTTTA GCGCCTCGTG ATCGTCTTTT 12300

GGAGGCTCGA TTAACGCGGG CACCAGCGAT TGAGCAGCTG TTTCAACTTT TCGCACGTAG 12360

CCGTTTGCAA AACCGCCGAT GAAATTACCG GTGTTGTAAG CGGAGATCGC CCGACGAAGC 12420

GCAAATTGCT TCTCGTCAAT CGTTTCGCCG CCTGCATAAC GACTTTTCAG CATGTTTGCA 12480

GCGGCAGATA ATGATGTGCA CGCCTGGAGC GCACCGTCAG GTGTCAGACC GAGCATAGAA 12540

AAATTTCGAG AGTTTATTTG CATGAGGCCA ACATCCAGCG AATGCCGTGC ATCGAGACGG 12600

TGCCTGACGA CTTGGGTTGC TTGGCTGTGA TCTTGCCAGT GAAGCGTTTC GCCGGTCGTG 12660

TTGTCATGAA TCGCTAAAGG ATCAAAGCGA CTCTCCACCT TAGCTATCGC CGCAAGCGTA 12720

GATGTCGCAA CTGATGGGGC ACACTTGCGA GCAACATGGT CAAACTCAGC AGATGAGAGT 12780

GGCGTGGCAA GGCTCGACGA ACAGAAGGAG ACCATCAAGG CAAGAGAAAG CGACCCCGAT 12840

CTCTTAAGCA TACCTTATCT CCTTAGCTCG CAACTAACAC CGCCTCTCCC GTTGGAAGAA 12900

GTGCGTTGTT TTATGTTGAA GATTATCGGG AGGGTCGGTT ACTCGAAAAT TTTCAATTGC 12960

TTCTTTATGA TTTCAATTGA AGCGAGAAAC CTCGCCCGGC GTCTTGGAAC GCAACATGGA 13020

CCGAGAACCG CGCATCCATG ACTAAGCAAC CGGATCGACC TATTCAGGCC GCAGTTGGTC 13080

AGGTCAGGCT CAGAACGAAA ATGCTCGGCG AGGTTACGCT GTCTGTAAAC CCATTCGATG 13140

AACGGGAAGC TTCCTTCCGA TTGCTCTTGG CAGGAATATT GGCCCATGCC TGCTTGCGCT 13200

TTGCAAATGC TCTTATCGCG TTGGTATCAT ATGCCTTGTC CGCCAGCAGA AACGCACTCT 13260

AAGCGATTAT TTGTAAAAAT GTTTCGGTCA TGCGGCGGTC ATGGGCTTGA CCCGCTGTCA 13320

GCGCAAGACG GATCGGTCAA CCGTCGGCAT CGACAACAGC GTGAATCTTG GTGGTCAAAC 13380

CGCCACGGGA ACGTCCCATA CAGCCATCGT CTTGATCCCG CTGTTTCCCG TCGCCGCATG 13440

TTGGTGGACG CGGACACAGG AACTGTCAAT CATGACGACA TTCTATCGAA AGCCTTGGAA 13500

ATCACACTCA GAATATGATC CCAGACGTCT GCCTCACGCC ATCGTACAAA GCGATTGTAG 13560CAGGTTGTAC AGGAACCGTA TCGATCAGGACGCCACAAGA TGACATTGAT CACCCGCGTCGAACAAAGGA CTGAACAACA GTCCATTCGATCAGTGGCCT CCAAGTCAAG CCTCAATGAATATGAGTGTG CGGCCTAAAT GATGAAATCGCACCTCGCAG TATCGCCGTG CTGACCTTGGCATGACCGCT CTTTTGGCCG CGATAGATGAGAGAAGTCAT ATCGGAGAAA TTCCTCCTGGCCACTGTCGG GAACAGACCG GATCATTCACGGCATCTTCC CTTGAAGGAT GATCTTGTTGCAGATGCGAT CTCAGCGCAA CTTGCGGCAAAGTCTCGCGA TCAGACGAAG GCCTTTTACTAGGCGTCGCT ATCCCAGTCA ATACTAAAGCAGGCGTGCCA CGAGGCCTGA GACGACGCGCTGATGGCCTC CGCTGAAGCC TATCACCTCTTTATTACGGT CTTCGCGCCC GTACATGCATTCATCCAGAG GATTGCCGCC CTTACCTTCCACGACATAGT CGACTTGATG TGACAATGCCTGCTCAAGCG TAAGCCTATT GAAGCTTGCCACAAGTAAAA CATTCTGCAC ACCGAAATGCTCCTTAGCAG TTTCGCTTGG GGACCGCTCCATGACAGGAA TCCCTTCCGT CTGCAGATAGTTTCGGTGAT GACGGTGAAA ACCTCTGACATCTGTAAGCG GATGCCGGGA GCAGACAAGCGTGTCGGGGC GCAGCCATGA CCCAGTCACGTATGCGGCAT CAGAGCAGAT TGTACTGAGAAGATGCGTAA GGAGAAAATA CCGCATCAGGCTGCGCTCGG TCGTTCGGCT GCGGCGAGCGTTATCCACAG AATCAGGGGA TAACGCAGGAGCCAGGAACC GTAAAAAGGC CGCGTTGCTGGAGCAT CACA AAAATCGACG C T CAAGT CAGTACCAGGCGT TTCCCCCTGG AAGCTCCCTCACCGGATACC TGTCCGCCTT TCTCCCTTCGTGTAGGTATC TCAGTTCGGT GTAGGTCGTTCCCGTTCAGC CCGACCGCTG CGCCTTATCCAGACACGACT TATCGCCACT GGCAGCAGCCGTAGGCGGTG CTACAGAGTT CTTGAAGTGGGTATTTGGTA TCTGCGCTCT GCTGAAGCCATGATCCGGCA AACAAACCAC CGCTGGTAGCACGCGCAGAA AAAAAGGATC TCAAGAAGATCAGTGGAACG AAAACTCACG TTAAGGGATTACCTAGATCC TTTTAAATTA AAAATGAAGTACTTGGTCTG ACAGTTACCA ATGCTTAATCTTTCGTTCAT CCATAGTTGC CTGACTCCCCTTACCATCTG GCCCCAGTGC TGCAATGATATTATCAGCAA TAAACCAGCC AGCCGGAAGGTCCGCCTCCA TCCAGTCTAT TAATTGTTGCAATAGTTTGC GCAACGTTGT TGCCATTGCTTCATTCCACG GACAAAAACA GAGAAAGGAAACCTGTCGTT TCCTTTCTTT TCAGAGGGTAGAAGAACGGA AACGCCTTAA ACCGGAAAATCCGCCCCGTA ACCTGTCGGA TCACCGGAAAACATATCACA ACGTGCGTGG AGGCCATCAATATCGTATTA ATTGATCTGC ATCAACTTAAGCGACACTGA ATACGGGGCA ACCTCATGTCGTGTCACGCT CGTCGTTTGG TATGGCTTCAGTTACATGAT CCCCCATGTT GTGCAAAAAAGTCAGAAGTA AGTTGGCCGC AGTGTTATCACTTACTGTCA TGCCATCCGT AAGATGCTTT

ACGTCTGCCC AGGGCGGGCC CGTCCGGAAG 13620

AACGCGCGGC ACGCGACGCG GCTTATTTGG 13680

AATCGGTGAC ATCAAAGCGG GGACGGGTTA 13740

TCAAAATCAG ACCGATTTGC AAACCTGATT 13800

TCCTTCTAGA TCGCCTCCGT GGTGTAGCAA 13860

CCAGGGAATT GACTGGCAAG GGTGCTTTCA 13920

TTTCGTTGCT GCTTTGGGCA CGTAGAAGGA 13980

CGCGAGAGCC TGCTCTATCG CGACGGCATC 14 040

GAGGCGAAAG TCGTCAACAC ATGCGTTATA 14100

CTGCCAATCT GGAGGTGCGG CAGCCGCAGG 14160

AACATCTCAC TCACCTGAAA ACCACTAGCG 14 220

TAACGACACA ATATCCGATG TCTGCATCAC 14 280

GGTGCAGGAA CTAAAGATTA CTGATGACTT 14 340

GTAGACAGTT TTTTGAAATC ATTATCAAAG 14 400

GCGCCGGTCT GTCGGAGAGA TGGGCAAGCA 14 4 60

TGGACGATTG CAGGGTCAAT GGATCTGAGA 14520

GTTTCGAGTT GGAGCCAGCC CCTAAATGAG 14 580

AAGAGAGAGA TTTGCTTAAC CCGATTTTTT 14 640

GGCATGACGT CCGCGCCGAA AGAATATCCT 14 7 00

TTGGTGTAGA CATCGATTAT GTGACCAAGA 14 7 60

GACCAGAAAT ACCGAAGTGA ACTGACGCCA 14 820

GTACCATCGA TAGATCTGCT GCCTCGCGCG 14880

CATGCAGCTC CCGGAGACGG TCACAGCTTG 14 940

CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 15000

TAGCGATAGC GGAGTGTATA CTGGCTTAAC 15060

GTGCACCATA TGCGGTGTGA AATACCGCAC 15120

CGCTCTTCCG CTTCCTCGCT CACTGACTCG 15180

GTATCAGCTC ACTCAAAGGC GGTAATACGG 1524 0

AAGAACATGT GAGCAAAAGG CCAGCAAAAG 15300

GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 15360

AGGTGGCGAA ACCCGACAGG ACTATAAAGA 154 20

GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 15480

GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 1554 0

CGCTCCAAGC TGGGCTGTGT GCACGAACCC 15600

GGTAACTATC GTCTTGAGTC CAACCCGGTA 15660

ACTGGTAACA GGATTAGCAG AGCGAGGTAT 15720

TGGCCTAACT ACGGCTACAC TAGAAGGACA 15780

GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 1584 0

GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 15900

CCTTTGATCT TTTCTACGGG GTCTGACGCT 15960

TTGGTCATGA GATTATCAAA AAGGATCTTC 16020

TTTAAATCAA TCTAAAGTAT ATATGAGTAA 16080

AGTGAGGCAC CTATCTCAGC GATCTGTCTA 1614 0

GTCGTGTAGA TAACTACGAT ACGGGAGGGC 16200

CCGCGAGACC CACGCTCACC GGCTCCAGAT 16260

GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 16320

CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 16380

GCAGGGGGGG GGGGGGGGGG GTTCCATTGT 16440

ACGACAGAGG CCAAAAAGCT CGCTTTCAGC 16500

TTTTAAATAA AAACATTAAG TTATGACGAA 16560

TTTCATAAAT AGCGAAAACC CGCGAGGTCG 16620

GGACCCGTAA AGTGATAATG ATTATCATCT 16680

ACCACGTCAA ATAATCAATT ATGACGCAGG 16740

CGTAAAAACA ACTTCAGACA ATACAAATCA 16800

CCCCCCCCCC CCCCCCCTGC AGGCATCGTG 16860

TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 16920

GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 16980

CTCATGGTTA TGGCAGCACT GCATAATTCT 1704 0

TCTGTGACTG GTGAGTACTC AACCAAGTCA 17100TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT 17160

ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 17220

AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 17280

AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 17340

CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 17 400

CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 17 4 60

GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 17520

CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 17 580

AGGCCCTTTC GTCTTCAAGA ATTGGTCGAC GATCTTGCTG CGTTCGGATA TTTTCGTGGA 17640

GTTCCCGCCA CAGACCCGGA TTGAAGGCGA GATCCAGCAA CTCGCGCCAG ATCATCCTGT 17700

GACGGAACTT TGGCGCGTGA TGACTGGCCA GGACGTCGGC CGAAAGAGCG ACAAGCAGAT 17 760

CACGCTTTTC GACAGCGTCG GATTTGCGAT CGAGGATTTT TCGGCGCTGC GCTACGTCCG 17820

CGACCGCGTT GAGGGATCAA GCCACAGCAG CCCACTCGAC CTTCTAGCCG ACCCAGACGA 17 880

GCCAAGGGAT CTTTTTGGAA TGCTGCTCCG TCGTCAGGCT TTCCGACGTT TGGGTGGTTG 17940

AACAGAAGTC ATTATCGTAC GGAATGCCAA GCACTCCCGA GGGGAACCCT GTGGTTGGCA 18000

TGCACATACA AATGGACGAA CGGATAAACC TTTTCACGCC CTTTTAAATA TCCGTTATTC 18060

TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT 18120

TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT 18180

ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA 18240

CGTTGAAGGA GCCACTCAGC AAGCTGGTAC GATTGTAATA CGACTCACTA TAGGGCGAAT 18300

TGAGCGCTGT TTAAACGCTC TTCAACTGGA AGAGCGGTTA CCAGAGCTGG TCACCTTTGT 18360

CCACCAAGAT GGAACTGCGG CCGCTCATTA ATTAAGTCAG GCGCGCCTCT AGTTGAAGAC 18420

ACGTTCATGT CTTCATCGTA AGAAGACACT CAGTAGTCTT CGGCCAGAAT GGCCCGGACC 18480

GAAGCTGGCC GCTCTAGAAC TAGTGGATCT CGATGTGTAG TCTACGAGAA GGGTTAACCG 1854 0

TCTCTTCGTG AGAATAACCG TGGCCTAAAA ATAAGCCGAT GAGGATAAAT AAAATGTGGT 18600

GGTACAGTAC TTCAAGAGGT TTACTCATCA AGAGGATGCT TTTCCGATGA GCTCTAGTAG 18 660

TACATCGGAC CTCACATACC TCCATTGTGG TGAAATATTT TGTGCTCATT TAGTGATGGG 18720

TAAATTTTGT TTATGTCACT CTAGGTTTTG ACATTTCAGT TTTGCCACTC TTAGGTTTTG 18780

ACAAATAATT TCCATTCCGC GGCAAAAGCA AAACAATTTT ATTTTACTTT TACCACTCTT 18840

AGCTTTCACA ATGTATCACA AATGCCACTC TAGAAATTCT GTTTATGCCA CAGAATGTGA 18 900

AAAAAAACAC TCACTTATTT GAAGCCAAGG TGTTCATGGC ATGGAAATGT GACATAAAGT 18 960

AACGTTCGTG TATAAGAAAA AATTGTACTC CTCGTAACAA GAGACGGAAA CATCATGAGA 19020

CAATCGCGTT TGGAAGGCTT TGCATCACCT TTGGATGATG CGCATGAATG GAGTCGTCTG 19080

CTTGCTAGCC TTCGCCTACC GCCCACTGAG TCCGGGCGGC AACTACCATC GGCGAACGAC 1914 0

CCAGCTGACC TCTACCGACC GGACTTGAAT GCGCTACCTT CGTCAGCGAC GATGGCCGCG 19200

TACGCTGGCG ACGTGCCCCC GCATGCATGG CGGCACATGG CGAGCTCAGA CCGTGCGTGG 19260

CTGGCTACAA ATACGTACCC CGTGAGTGCC CTAGCTAGAA ACTTACACCT GCAACTGCGA 19320

GAGCGAGCGT GTGAGTGTAG CCGAGTAGAT CCCCCGGTCG CCACCATGGC CTCCTCCGAG 19380

AACGTCATCA CCGAGTTCAT GCGCTTCAAG GTGCGCATGG AGGGCACCGT GAACGGCCAC 19440

GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCCACAA CACCGTGAAG 19500

CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC 19560

CAGTACGGCT CCAAGGTGTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG 19620

TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGCG 19680

ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCTGCTTCA TCTACAAGGT GAAGTTCATC 19740

GGCGTGAACT TCCCCTCCGA CGGCCCCGTG ATGCAGAAGA AGACCATGGG CTGGGAGGCC 19800

TCCACCGAGC GCCTGTACCC CCGCGACGGC GTGCTGAAGG GCGAGACCCA CAAGGCCCTG 198 60

AAGCTGAAGG ACGGCGGCCA CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG 19920

CCCGTGCAGC TGCCCGGCTA CTACTACGTG GACGCCAAGC TGGACATCAC CTCCCACAAC 19980

GAGGACTACA CCATCGTGGA GCAGTACGAG CGCACCGAGG GCCGCCACCA CCTGTTCCTG 20040

TAGCGGCCCA TGGATATTCG AACGCGTAGG TACCACATGG TTAACCTAGA CTTGTCCATC 20100

TTCTGGATTG GCCAACTTAA TTAATGTATG AAATAAAAGG ATGCACACAT AGTGACATGC 20160

TAATCACTAT AATGTGGGCA TCAAAGTTGT GTGTTATGTG TAATTACTAG TTATCTGAAT 20220

AAAAGAGAAA GAGATCATCC ATATTTCTTA TCCTAAATGA ATGTCACGTG TCTTTATAAT 20280

TCTTTGATGA ACCAGATGCA TTTCATTAAC CAAATCCATA TACATATAAA TATTAATCAT 20340

ATATAATTAA TATCAATTGG GTTAGCAAAA CAAATCTAGT CTAGGTGTGT TTTGCGAATG 20400

CGGCCGCCAC CGCGGTGGAG CTCGAATTCC GGTCCGGGCC TAGAAGGCCA TTTAAATCCT 204 60

GAGGATCTGG TCTTCCTAAG GACCCGGGAT ATCGCTATCA ACTTTGTATA GAAAAGTTGG 20520

GCCGAATTCG AGCTCGGTAC GGCCAGAATG GCCCGGACCG GGTTACCGAA TTCGAGCTCG 20580

GTACCCTGGG ATCCGCAAGG GACGACCGTG GTCCTTGTTT GATTTACTTC CAGGATTATA 20640TAATCCAGCT TATGGATTAT ATAAGTACCT ATTGACGTCA CGTGCTTATG TATTATAATA 20700

ATCTAGGTAT ATAGATTATA TAATCTATCT AATAATAATC TGTGTTGTTT GTTTATCTCT 207 60

CAAAACAAAC AGGTCCTAAA ATGGTCCCGG GCGTCCAATG TGTCGTCAAG TAGTGTTAAG 20820

CTAAATCGAC ATTTCTTTGT GGGTTGTGTG GAAGGTGTTC CTTTTCCTTA AGTTGTTAGT 20880

TGTGCAAGGT GTTCCTTAGA GCATCTCCAA TAGGACCTAT AATGGATTCT ATTTTGAATT 20940

ATAAGACTCT AACAACAAAA GCATACTTTA ATGGGGATTC TATTTTACAA AAAAATATCA 21000

AATGATTATA TGGTCGATTC CTCGGGTCCT AAATATAGTA TCTCATATAA TAGAGCTCTA 21060

TCCTCATTTT ATATACTATT TTTAAGTTTT TATTTACTAA ATAACATGAT TTATTTTCTA 21120

ATACTATGAA CTCAACTATT AGAGCTGTAA ACGTTTTTGT GGTACTAAAC ACTTTAAATC 21180

AGGTCCTATT TTAATTTGAA GGACTTAAAT ATAAGACTTC TGGTTAGAGA TGCTCTTAGC 2124 0

GAGTGTTTGT GCATGATTGC TATTTAGTCT TTGTGGATTG TGGAAGGTGT TACTTTTCCT 21300

CAAGTTGTTA GTTGTGCAAG GTGTTTCTTA GAGCATCTCT AACAGGAGCC TTAACGGAAT 21360

CTATTTTGAA GTATAGTACT TTAACACCAA AAACATACTT TAATAGGGGT CCTATTTTAC 214 20

AAAAAAATTA TCAAATGATT ATAAGGTCCA CTCCTCGGGT CCTAAATATA ATATCTCATA 214 80

TACTAGAGCT CTATCCTCAT TTTATATACT ATCCCTAGGT TTTTATTCCC TAAATAACAT 21540

GATTTATTTC CTAATACTAA GATATAGGGC TCAACTATTG GAGTTGCAAA TGTTTTTTGG 21600

CACTAAACAC TTTATATCAG GTCCTATTTT AATTTTAATT TGAAGGACTC AAATATAGGA 21660

CTTCTCGTTA GAGATGCTCT TAGCGAGTGT TTGTGCATGA TTGCTATTTA TGTCTGTAGT 21720

TTAGTTGGGG GCTTTAATAT GTTTAGTTGA AGTTCTAGTA TTTTTTAGGT TCTCCACTCT 21780

TTGGATTATG ACAACGACCA CTATCCAAGC AGTCTTTGAG TGCAAACGCG CGAGCAAACT 21840

ATCTGATCTA TTAAATTATG ATCCAACCGT TATGTCATAT TGAAGACTTA AACCCTTTCA 21900

CCACCAGCCC AAGTATCTTT ATGAAAAACC CTAACAAACC ACAATTGCAT CTATGGTTGG 21960

ATTATAATTT AACGTATCAG ATGGTTCGCT TGCATGCTTA CATATCTAGA AACTGTTTGC 22020

ATAACAGTCG TTCTCTTTGG TTATATAATG CTTTAGTAAT CATCAGCCAA GTGTAAACAA 22080

ATGGTACAAA CTAGTAGTGA ACACATCCTC CCTACCTATC TCTAGGGGTG TAACTAGATA 2214 0

TCCGAATTCT TAGAACAAAT TTCATATTTT AAAATAGATA TGCTTCAAAA TTTATGCTAA 22200

TCTTTTTTAT ATTATCAAGC ATATTATTAC ACATAAGAAT AAAATTTTGT ATAGAATTTT 22260

ATCCATTATT TGTTCCCTAG AATTTAAAAA GTGAAAAAAC ATTCGAATCT GTATCAGTTT 22320

CGTATTCAAA TTTTTACATC TATTATTTGA GAATATATAT GATAAATTTG AGGTTTAGTT 22380

TTTATGAATC TTTACAAGGT TAATGTTAAA TACATGACTA TGGATTTACA TAGTAAATTC 22440

TATGTCTTAT TTGTCCGCGA TTGAAGAAAA ATGACAAAAA GATCTGACAT TCGAATAAAC 22500

ATCTGTTTCC ACTCCTACCT ATCTGACCTC CTATTTCAAA CTCCACTTTG TAACACGGTA 22560

CAAAATCACT CCCTACCTAT CTGACCTCCT ATTTCAAACT CCACTCAGTA AACAATATTG 22 620

TCTATGGTAC AAAACCAAGT GTTTTATACA TCTATTTGCA CGATCTGCTC GAGTCAGGCA 22 680

TCCTTGACAC ACAACATACT CCTTGTGGCT ATAAATGTCC AAATAGAGCA GACCTAATGG 2274 0

GTGGACCGTT GCATGACACG ACTTATCCCA AGACGAGCAC AGTTCGCCCC ATTGGTCATG 22800

GGGGTCCGGG CTAGTCTAGC CTGATCATCG GGTCACACTT AGGCCACAGG TGTGCCACAA 228 60

CGGGATAGCC CAACATGTCC CTTTTTGTCA TGCATATATC TATATTATAG TTAGTATAAT 22 920

GTAAAAAAAC AAAAGGTATG TGTGTTATGT TGGTTAGATG TGTTTAAATA ACTCTTTAAA 22980

GCTAGCAACT ATGGTTTAAA TCATACATAT ACACATTTTT ATTTTATTTT TATTTAAACG 23040

ATATGGGCCT TCTAGGCACG TCGAGTGTGA CGGGCCAGTG AGATGACACA TTATAATTAC 23100

TGGTCTAGCA GGCCGTACCT AGGTCTTTCT CGTGGGCCAA GACTAAGGGT TGGCCCGTTG 23160

GCTAATCTGT ACGGTACCGA TACTGTCCTA ATTCATTTGA ACACCTGTAG AAGAGGGGAA 23220

TTTATAATTG AGGAGGAATG TACTCATGCG GTACACCAGG GGAATTGTTT TGTTGTGCTC 23280

AGCGATAGAT TTCAACGCAA CGGTGAGCCA GTTTCACTAA AAAAAGGGGG GGGGGGGGGG 2334 0

GGGGGGGAAG GCCACATCAA AGGCGAGGTG CTGACGAGCA GAAGATGCTA GCAGTGACGC 23400

CAAGTCCAGC AGCTAGCAAT GAAAGGGTAC TCGGGATTTA ACAATGCCTA GAGACGGCAT 23460

CATCCCCTCA ATAATCCGGT GCTCTCTTTT TGTTTATTCA CCAGTTGGCG TAGCTATATA 23520

CACATGTCTG GTCTGACGAA CAAATCAAGG GATCGCTAGC TCGGGCTAGC CTTCCTATCA 23580

CTGTCATGAC ATGTGCTCTG CCTCTGCTGG TTGATAAGCC GTGCGCCTTC TCGCTAATTC 23640

TTTCTTGTGC TAGAGGCGAG TCAAACAAAC GCTGCACCTC GTAGCCCTTA ATCTGCGCTA 23700

AGGGTCACAT GACCCTGTTC CCTATCGCTA GTTACCAACG ACCCATTCCC CCTGACAGAT 237 60

ACTTACGACG CGTCCGTACG CGGCAGGCCT CGGCAGTTCG GCATCACCAG CACCGGCGCC 23820

GGCATTCGCC CCCTGCCAGC CGGTTCGCAG ATTCGCAGGG CGGAGTCGGC CGCAGTTGCC 23880

GCATCCCAAA CGCCCGGGAA CCTTTGGGGC CCCTCTACGA GCAAATGAAG TTGCTGCCCC 23940

TGGCTTCGTA AAGCTCTGAC TTTTGATCAC TTGATTGGCA GTCGTACTCC TCGCTCATAG 24 000

GCCGACACGG CCGCAAAGTC AACTACCCGC TCCGCCATCC TTCAACCCCC GCCACGCGCC 24 060

TATATATGTT CGCGGCCATG TCCGTACTAG TCCTCCAACC CACAAGCCAC AACCCCGAGC 24120

TCAGATCCCT CGCCTCGTGT CGTGTCTCCG GTCGACGACG ACCAACAGCC AGTGTGGGCC 24180AGACGGACAC CGCCGAGCTA TAGCGCTTGG TGATAAAGCT TGGTCACCCG GTCCGGGCCT 24240

AGAAGGCCAG CTTCAAGTTT GTACAAAAAA GCAGGCTCCA GCGCTCACCA TGGTCCGTCC 24 300

TGTAGAAACC CCAACCCGTG AAATCAAAAA ACTCGACGGC CTGTGGGCAT TCAGTCTGGA 24 360

TCGCGAAAAC TGTGGAATTG ATCAGCGTTG GTGGGAAAGC GCGTTACAAG AAAGCCGGGC 24 420

AATTGCTGTG CCAGGCAGTT TTAACGATCA GTTCGCCGAT GCAGATATTC GTAATTATGC 24 4 80

GGGCAACGTC TGGTATCAGC GCGAAGTCTT TATACCGAAA GGTTGGGCAG GCCAGCGTAT 24 540

CGTGCTGCGT TTCGATGCGG TCACTCATTA CGGCAAAGTG TGGGTCAATA ATCAGGAAGT 24 600

GATGGAGCAT CAGGGCGGCT ATACGCCATT TGAAGCCGAT GTCACGCCGT ATGTTATTGC 24 660

CGGGAAAAGT GTACGTAAGT TTCTGCTTCT ACCTTTGATA TATATATAAT AATTATCATT 24 720

AATTAGTAGT AATATAATAT TTCAAATATT TTTTTCAAAA TAAAAGAATG TAGTATATAG 247 80

CAATTGCTTT TCTGTAGTTT ATAAGTGTGT ATATTTTAAT TTATAACTTT TCTAATATAT 24 840

GACCAAAATT TGTTGATGTG CAGGTATCAC CGTTTGTGTG AACAACGAAC TGAACTGGCA 24 900

GACTATCCCG CCGGGAATGG TGATTACCGA CGAAAACGGC AAGAAAAAGC AGTCTTACTT 24 960

CCATGATTTC TTTAACTATG CCGGAATCCA TCGCAGCGTA ATGCTCTACA CCACGCCGAA 25020

CACCTGGGTG GACGATATCA CCGTGGTGAC GCATGTCGCG CAAGACTGTA ACCACGCGTC 25080

TGTTGACTGG CAGGTGGTGG CCAATGGTGA TGTCAGCGTT GAACTGCGTG ATGCGGATCA 25140

ACAGGTGGTT GCAACTGGAC AAGGCACTAG CGGGACTTTG CAAGTGGTGA ATCCGCACCT 25200

CTGGCAACCG GGTGAAGGTT ATCTCTATGA ACTGTGCGTC ACAGCCAAAA GCCAGACAGA 25260

GTGTGATATC TACCCGCTTC GCGTCGGCAT CCGGTCAGTG GCAGTGAAGG GCGAACAGTT 25320

CCTGATTAAC CACAAACCGT TCTACTTTAC TGGCTTTGGT CGTCATGAAG ATGCGGACTT 25380

GCGTGGCAAA GGATTCGATA ACGTGCTGAT GGTGCACGAC CACGCATTAA TGGACTGGAT 25440

TGGGGCCAAC TCCTACCGTA CCTCGCATTA CCCTTACGCT GAAGAGATGC TCGACTGGGC 25500

AGATGAACAT GGCATCGTGG TGATTGATGA AACTGCTGCT GTCGGCTTTA ACCTCTCTTT 25560

AGGCATTGGT TTCGAAGCGG GCAACAAGCC GAAAGAACTG TACAGCGAAG AGGCAGTCAA 25620

CGGGGAAACT CAGCAAGCGC ACTTACAGGC GATTAAAGAG CTGATAGCGC GTGACAAAAA 25680

CCACCCAAGC GTGGTGATGT GGAGTATTGC CAACGAACCG GATACCCGTC CGCAAGGTGC 257 4 0

ACGGGAATAT TTCGCGCCAC TGGCGGAAGC AACGCGTAAA CTCGACCCGA CGCGTCCGAT 25800

CACCTGCGTC AATGTAATGT TCTGCGACGC TCACACCGAT ACCATCAGCG ATCTCTTTGA 25860

TGTGCTGTGC CTGAACCGTT ATTACGGATG GTATGTCCAA AGCGGCGATT TGGAAACGGC 25920

AGAGAAGGTA CTGGAAAAAG AACTTCTGGC CTGGCAGGAG AAACTGCATC AGCCGATTAT 25980

CATCACCGAA TACGGCGTGG ATACGTTAGC CGGGCTGCAC TCAATGTACA CCGACATGTG 2 6040

GAGTGAAGAG TATCAGTGTG CATGGCTGGA TATGTATCAC CGCGTCTTTG ATCGCGTCAG 2 6100

CGCCGTCGTC GGTGAACAGG TATGGAATTT CGCCGATTTT GCGACCTCGC AAGGCATATT 26160

GCGCGTTGGC GGTAACAAGA AAGGGATCTT CACTCGCGAC CGCAAACCGA AGTCGGCGGC 2 6220

TTTTCTGCTG CAAAAACGCT GGACTGGCAT GAACTTCGGT GAAAAACCGC AGCAGGGAGG 26280

CAAACAATGA AGATCTCCCG GGCACCCAGC TTTCTTGTAC AAAGTGGCCG TTAACGGATC 2 6340

CAGACTTGTC CATCTTCTGG ATTGGCCAAC TTAATTAATG TATGAAATAA AAGGATGCAC 26400

ACATAGTGAC ATGCTAATCA CTATAATGTG GGCATCAAAG TTGTGTGTTA TGTGTAATTA 264 60

CTAGTTATCT GAATAAAAGA GAAAGAGATC ATCCATATTT CTTATCCTAA ATGAATGTCA 26520

CGTGTCTTTA TAATTCTTTG ATGAACCAGA TGCATTTCAT TAACCAAATC CATATACATA 26580

TAAATATTAA TCATATATAA TTAATATCAA TTGGGTTAGC AAAACAAATC TAGTCTAGGT 26640

GTGTTTTGCG AATTGCGGCA AGCTTGCGGC CGCCCCGGGC AACTTTATTA TACAAAGTTG 2 6700

ATAGATATCG GACCGATTAA ACTTTAATTC GGTCCGAAGC TTGCATGCCT GCAGTGCAGC 267 60

GTGACCCGGT CGTGCCCCTC TCTAGAGATA ATGAGCATTG CATGTCTAAG TTATAAAAAA 2 6820

TTACCACATA TTTTTTTTGT CACACTTGTT TGAAGTGCAG TTTATCTATC TTTATACATA 2 6880

TATTTAAACT TTACTCTACG AATAATATAA TCTATAGTAC TACAATAATA TCAGTGTTTT 2694 0

AGAGAATCAT ATAAATGAAC AGTTAGACAT GGTCTAAAGG ACAATTGAGT ATTTTGACAA 27000

CAGGACTCTA CAGTTTTATC TTTTTAGTGT GCATGTGTTC TCCTTTTTTT TTGCAAATAG 27060

CTTCACCTAT ATAATACTTC ATCCATTTTA TTAGTACATC CATTTAGGGT TTAGGGTTAA 27120

TGGTTTTTAT AGACTAATTT TTTTAGTACA TCTATTTTAT TCTATTTTAG CCTCTAAATT 27180

AAGAAAACTA AAACTCTATT TTAGTTTTTT TATTTAATAA TTTAGATATA AAATAGAATA 2724 0

AAATAAAGTG ACTAAAAATT AAACAAATAC CCTTTAAGAA ATTAAAAAAA CTAAGGAAAC 27300

ATTTTTCTTG TTTCGAGTAG ATAATGCCAG CCTGTTAAAC GCCGTCGACG AGTCTAACGG 27360

ACACCAACCA GCGAACCAGC AGCGTCGCGT CGGGCCAAGC GAAGCAGACG GCACGGCATC 27 4 20

I TCTGTCGCTG CCTCTGGACC CCTCTCGAGA GTTCCGCTCC ACCGTTGGAC TTGCTCCGCT 27 4 80

j GTCGGCATCC AGAAATTGCG TGGCGGAGCG GCAGACGTGA GCCGGCACGG CAGGCGGCCT 2754 0

1 CCTCCTCCTC TCACGGCACC GGCAGCTACG GGGGATTCCT TTCCCACCGC TCCTTCGCTT 27600

1 TCCCTTCCTC GCCCGCCGTA ATAAATAGAC ACCCCCTCCA CACCCTCTTT CCCCAACCTC 27 660

GTGTTGTTCG GAGCGCACAC ACACACAACC AGATCTCCCC CAAATCCACC CGTCGGCACC 27720TCCGCTTCAA GGTACGCCGC TCGTCCTCCC CCCCCCCCCT CTCTACCTTC TCTAGATCGG 27780

CGTTCCGGTC CATGCATGGT TAGGGCCCGG TAGTTCTACT TCTGTTCATG TTTGTGTTAG 27840

ATCCGTGTTT GTGTTAGATC CGTGCTGCTA GCGTTCGTAC ACGGATGCGA CCTGTACGTC 27900

AGACACGTTC TGATTGCTAA CTTGCCAGTG TTTCTCTTTG GGGAATCCTG GGATGGCTCT 27960

AGCCGTTCCG CAGACGGGAT CGATTTCATG ATTTTTTTTG TTTCGTTGCA TAGGGTTTGG 28020

TTTGCCCTTT TCCTTTATTT CAATATATGC CGTGCACTTG TTTGTCGGGT CATCTTTTCA 28080

TGCTTTTTTT TGTCTTGGTT GTGATGATGT GGTCTGGTTG GGCGGTCGTT CTAGATCGGA 2814 0

GTAGAATTCT GTTTCAAACT ACCTGGTGGA TTTATTAATT TTGGATCTGT ATGTGTGTGC 28200

CATACATATT CATAGTTACG AATTGAAGAT GATGGATGGA AATATCGATC TAGGATAGGT 28260

ATACATGTTG ATGCGGGTTT TACTGATGCA TATACAGAGA TGCTTTTTGT TCGCTTGGTT 28320

GTGATGATGT GGTGTGGTTG GGCGGTCGTT CATTCGTTCT AGATCGGAGT AGAATACTGT 28380

TTCAAACTAC CTGGTGTATT TATTAATTTT GGAACTGTAT GTGTGTGTCA TACATCTTCA 28440

TAGTTACGAG TTTAAGATGG ATGGAAATAT CGATCTAGGA TAGGTATACA TGTTGATGTG 28500

GGTTTTACTG ATGCATATAC ATGATGGCAT ATGCAGCATC TATTCATATG CTCTAACCTT 28560

GAGTACCTAT CTATTATAAT AAACAAGTAT GTTTTATAAT TATTTTGATC TTGATATACT 28620

TGGATGATGG CATATGCAGC AGCTATATGT GGATTTTTTT AGCCCTGCCT TCATACGCTA 28680

TTTATTTGCT TGGTACTGTT TCTTTTGTCG ATGCTCACCC TGTTGTTTGG TGTTACTTCT 28740

GCAGGTCGAC TTTAACTTAG CCTAGGATCC ACACGACACC ATGTCCCCCG AGCGCCGCCC 28800

CGTCGAGATC CGCCCGGCCA CCGCCGCCGA CATGGCCGCC GTGTGCGACA TCGTGAACCA 28860

CTACATCGAG ACCTCCACCG TGAACTTCCG CACCGAGCCG CAGACCCCGC AGGAGTGGAT 28920

CGACGACCTG GAGCGCCTCC AGGACCGCTA CCCGTGGCTC GTGGCCGAGG TGGAGGGCGT 28980

GGTGGCCGGC ATCGCCTACG CCGGCCCGTG GAAGGCCCGC AACGCCTACG ACTGGACCGT 2904 0

GGAGTCCACC GTGTACGTGT CCCACCGCCA CCAGCGCCTC GGCCTCGGCT CCACCCTCTA 2 9100

CACCCACCTC CTCAAGAGCA TGGAGGCCCA GGGCTTCAAG TCCGTGGTGG CCGTGATCGG 2 9160

CCTCCCGAAC GACCCGTCCG TGCGCCTCCA CGAGGCCCTC GGCTACACCG CCCGCGGCAC 29220

CCTCCGCGCC GCCGGCTACA AGCACGGCGG CTGGCACGAC GTCGGCTTCT GGCAGCGCGA 29280

CTTCGAGCTG CCGGCCCCGC CGCGCCCGGT GCGCCCGGTG ACGCAGATCT GAGTCGAAAC 2 934 0

CTAGACTTGT CCATCTTCTG GATTGGCCAA CTTAATTAAT GTATGAAATA AAAGGATGCA 2 94 00

CACATAGTGA CATGCTAATC ACTATAATGT GGGCATCAAA GTTGTGTGTT ATGTGTAATT 294 60

ACTAGTTATC TGAATAAAAG AGAAAGAGAT CATCCATATT TCTTATCCTA AATGAATGTC 29520

ACGTGTCTTT ATAATTCTTT GATGAACCAG ATGCATTTCA TTAACCAAAT CCATATACAT 29580

ATAAATATTA ATCATATATA ATTAATATCA ATTGGGTTAG CAAAACAAAT CTAGTCTAGG 29640

TGTGTTTTGC GAATTGCGGC CGCCACCGCG GTGGAGCTCG AATTCATTCC GATTAATCGT 29700

GGCCTCTTGC TCTTCAGGAT GAAGAGCTAT GTTTAAACGT GCAAGCGCTA CTAGACAATT 297 60

CAGTACATTA AAAACGTCCG CAATGTGTTA TTAAGTTGTC TAAGCGTCAA TTTGTTTACA 29820

CCACAATATA TCCTGCCACC AGCCAGCCAA CAGCTCCCCG ACCGGCAGCT CGGCACAAAA 2 9880

TCACCACTCG ATACAGGCAG CCCATCAGTC CGGGACGGCG TCAGCGGGAG AGCCGTTGTA 2994 0

AGGCGGCAGA CTTTGCTCAT GTTACCGATG CTATTCGGAA GAACGGCAAC TAAGCTGCCG 30000

GGTTTGAAAC ACGGATGATC TCGCGGAGGG TAGCATGTTG ATTGTAACGA TGACAGAGCG 30060

TTGCTGCCTG TGATCAAATA TCATCTCCCT CGCAGAGATC CGAATTATCA GCCTTCTTAT 30120

TCATTTCTCG CTTAACCGTG ACAGGCTGTC GATCTTGAGA ACTATGCCGA CATAATAGGA 30180

AATCGCTGGA TAAAGCCGCT GAGGAAGCTG AGTGGCGCTA TTTCTTTAGA AGTGAACGTT 3024 0

GACGATCGTC GACCGTACCC CGATGAATTA ATTCGGACGT ACGTTCTGAA CACAGCTGGA 30300

TACTTACTTG GGCGATTGTC ATACATGACA TCAACAATGT ACCCGTTTGT GTAACCGTCT 30360

CTTGGAGGTT CGTATGACAC TAGTGGTTCC CCTCAGCTTG CGACTAGATG TTGAGGCCTA 30420

ACATTTTATT AGAGAGCAGG CTAGTTGCTT AGATACATGA TCTTCAGGCC GTTATCTGTC 304 80

AGGGCAAGCG AAAATTGGCC ATTTATGACG ACCAATGCCC CGCAGAAGCT CCCATCTTTG 3054 0

CCGCCATAGA CGCCGCGCCC CCCTTTTGGG GTGTAGAACA TCCTTTTGCC AGATGTGGAA 30600

AAGAAGTTCG TTGTCCCATT GTTGGCAATG ACGTAGTAGC CGGCGAAAGT GCGAGACCCA 30660

TTTGCGCTAT ATATAAGCCT ACGATTTCCG TTGCGACTAT TGTCGTAATT GGATGAACTA 30720

TTATCGTAGT TGCTCTCAGA GTTGTCGTAA TTTGATGGAC TATTGTCGTA ATTGCTTATG 30780

GAGTTGTCGT AGTTGCTTGG AGAAATGTCG TAGTTGGATG GGGAGTAGTC ATAGGGAAGA 30840

CGAGCTTCAT CCACTAAAAC AATTGGCAGG TCAGCAAGTG CCTGCCCCGA TGCCATCGCA 30900

AGTACGAGGC TTAGAACCAC CTTCAACAGA TCGCGCATAG TCTTCCCCAG CTCTCTAACG 30960

CTTGAGTTAA GCCGCGCCGC GAAGCGGCGT CGGCTTGAAC GAATTGTTAG ACATTATTTG 31020

CCGACTACCT TGGTGATCTC GCCTTTCACG TAGTGAACAA ATTCTTCCAA CTGATCTGCG 31080

CGCGAGGCCA AGCGATCTTC TTGTCCAAGA TAAGCCTGCC TAGCTTCAAG TATGACGGGC 31140

TGATACTGGG CCGGCAGGCG CTCCATTGCC CAGTCGGCAG CGACATCCTT CGGCGCGATT 31200

TTGCCGGTTA CTGCGCTGTA CCAAATGCGG GACAACGTAA GCACTACATT TCGCTCATCG 31260CCAGCCCAGT CGGGCGGCGA GTTCCATAGC GTTAAGGTTT CATTTAGCGC CTCAAATAGA 31320

TCCTGTTCAG-GAACCGGATC AAAGAGTTCC TCCGCCGCTG GACCTACCAA GGCAACGCTA 31380

TGTTCTCTTG CTTTTGTCAG CAAGATAGCC AGATCAATGT CGATCGTGGC TGGCTCGAAG 31440

ATACCTGCAA GAATGTCATT GCGCTGCCAT TCTCCAAATT GCAGTTCGCG CTTAGCTGGA 31500

TAACGCCACG GAATGATGTC GTCGTGCACA ACAATGGTGA CTTCTACAGC GCGGAGAATC 31560

TCGCTCTCTC CAGGGGAAGC CGAAGTTTCC AAAAGGTCGT TGATCAAAGC TCGCCGCGTT 31620

GTTTCATCAA GCCTTACAGT CACCGTAACC AGCAAATCAA TATCACTGTG TGGCTTCAGG 31680

CCGCCATCCA CTGCGGAGCC GTACAAATGT ACGGCCAGCA ACGTCGGTTC GAGATGGCGC 3174 0

TCGATGACGC CAACTACCTC TGATAGTTGA GTCGATACTT CGGCGATCAC CGCTTCCCTC 31800

ATGATGTTTA ACTCCTGAAT TAAGCCGCGC CGCGAAGCGG TGTCGGCTTG AATGAATTGT 31860

TAGGCGTCAT CCTGTGCTCC CGAGAACCAG TACCAGTACA TCGCTGTTTC GTTCGAGACT 31920

TGAGGTCTAG TTTTATACGT GAACAGGTCA ATGCCGCCGA GAGTAAAGCC ACATTTTGCG 31980

TACAAATTGC AGGCAGGTAC ATTGTTCGTT TGTGTCTCTA ATCGTATGCC AAGGAGCTGT 32040

CTGCTTAGTG CCCACTTTTT CGCAAATTCG ATGAGACTGT GCGCGACTCC TTTGCCTCGG 32100

TGCGTGTGCG ACACAACAAT GTGTTCGATA GAGGCTAGAT CGTTCCATGT TGAGTTGAGT 32160

TCAATCTTCC CGACAAGCTC TTGGTCGATG AATGCGCCAT AGCAAGCAGA GTCTTCATCA 32220

GAGTCATCAT CCGAGATGTA ATCCTTCCGG TAGGGGCTCA CACTTCTGGT AGATAGTTCA 32280

AAGCCTTGGT CGGATAGGTG CACATCGAAC ACTTCACGAA CAATGAAATG GTTCTCAGCA 3234 0

TCCAATGTTT CCGCCACCTG CTCAGGGATC ACCGAAATCT TCATATGACG CCTAACGCCT 324 00

GGCACAGCGG ATCGCAAACC TGGCGCGGCT TTTGGCACAA AAGGCGTGAC AGGTTTGCGA 324 60

ATCCGTTGCT GCCACTTGTT AACCCTTTTG CCAGATTTGG TAACTATAAT TTATGTTAGA 32520

GGCGAAGTCT TGGGTAAAAA CTGGCCTAAA ATTGCTGGGG ATTTCAGGAA AGTAAACATC 32580

ACCTTCCGGC TCGATGTCTA TTGTAGATAT ATGTAGTGTA TCTACTTGAT CGGGGGATCT 32 64 0

GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 32700

CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 327 60

CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT 32820

ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG 32880

TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC 32 94 0

GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 33000

GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 33060

AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 33120

CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 33180

AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 33240

GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 33300

TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 33360

TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 33420

GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 33480

CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 33540

CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 33600

AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 33660

CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 33720

GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 33780

AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 3384 0

TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 33900

AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 33960

GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 34 020

ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 34 080

TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 3414 0

TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGGG GGGGGGGGGG 34200

GGGGGACTTC CATTGTTCAT TCCACGGACA AAAACAGAGA AAGGAAACGA CAGAGGCCAA 34 260

AAAGCCTCGC TTTCAGCACC TGTCGTTTCC TTTCTTTTCA GAGGGTATTT TAAATAAAAA 34 320

CATTAAGTTA TGACGAAGAA GAACGGAAAC GCCTTAAACC GGAAAATTTT CATAAATAGC 34 380

GAAAACCCGC GAGGTCGCCG CCCCGTAACC TGTCGGATCA CCGGAAAGGA CCCGTAAAGT 34 440

GATAATGATT ATCATCTACA TATCACAACG TGCGTGGAGG CCATCAAACC ACGTCAAATA 34 500

ATCAATTATG ACGCAGGTAT CGTATTAATT GATCTGCATC AACTTAACGT AAAAACAACT 34 560

TCAGACAATA CAAATCAGCG ACACTGAATA CGGGGCAACC TCATGTCCCC CCCCCCCCCC 34620

CCCCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 34 680

CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 34 74 0

TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 34 800CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 34 860

AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 34 920

CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 34 980

AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 3504 0

AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 35100

GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 35160

GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 35220

TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 35280

TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA 35340

AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCAAGAATT CGGAGCTTTT GCCATTCTCA 35400

CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG 354 60

AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT 35520

GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA 35580

AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG 35640

TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA 35700

CGGGACGGCG GCTTTGTTGA ATAAATCGAA CTTTTGCTGA GTTGAAGGAT CAGATCACGC 357 60

ATCTTCCCGA CAACGCAGAC CGTTCCGTGG CAAAGCAAAA GTTCAAAATC ACCAACTGGT 35820

CCACCTACAA CAAAGCTCTC ATCAACCGTG GCTCCCTCAC TTTCTGGCTG GATGATGGGG 35880

CGATTCAGGC CTGGTATGAG TCAGCAACAC CTTCTTCACG AGGCAGACCT CAGCGCCAGA 35940

AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA 36000

AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT 36060

ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC 36120

CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT 36180

CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC 36240

GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC 36300

GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG 36360

CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC 36420

GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG 36480

GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC 36540

GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC 36600

GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA 36660

CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG 36720

GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT 36780

AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG 36840

CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC 36900

ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG 36960

CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT 37020

GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG 37080

CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCACTGTCG ACTACGCCAT CATGGCGACA 3714 0

GCGCCTTTCC TTTGGGTTCT CTATATCGGG CGGATCGTGG CCGGCATCAC CGGGGCGACT 37200

GGGGCGGTAG CCGGCGCTTA TATTGCCGAT ATCACTGATG GCGATGAGCG CGCGCGGCAC 37260

TTCGGCTTCA TGAGCGCCTG TTTCGGGTTC GGGATGGTCG CGGGACCTGT GCTCGGTGGG 37320

CTGATGGGCG GTTTCTCCCC CCACGCTCCG TTCTTCGCCG CGGCAGCCTT GAACGGCCTC 37380

AATTTCCTGA CGGGCTGTTT CCTTTTGCCG GAGTCGCACA AAGGCGAACG CCGGCCGTTA 374 4 0

CGCCGGGAGG CTCTCAACCC GCTCGCTTCG TTCCGGTGGG CCCGGGGCAT GACCGTCGTC 37 500

GCCGCCCTGA TGGCGGTCTT CTTCATCATG CAACTTGTCG GACAGGTGCC GGCCGCGCTT 37560

TGGGTCATTT TCGGCGAGGA TCGCTTTCAC TGGGACGCGA CCACGATCGG CATTTCGCTT 37 620

GCCGCATTTG GCATTCTGCA TTCACTCGCC CAGGCAATGA TCACCGGCCC TGTAGCCGCC 37 680

CGGCTCGGCG AAAGGCGGGC ACTCATGCTC GGAATGATTG CCGACGGCAC AGGCTACATC 377 40

CTGCTTGCCT TCGCGACACG GGGATGGATG GCGTTCCCGA TCATGGTCCT GCTTGCTTCG 37800

GGTGGCATCG GAATGCCGGC GCTGCAAGCA ATGTTGTCCA GGCAGGTGGA TGAGGAACGT 37 8 60

CAGGGGCAGC TGCAAGGCTC ACTGGCGGCG CTCACCAGCC TGACCTCGAT CGTCGGACCC 37 920

CTCCTCTTCA CGGCGATCTA TGCGGCTTCT ATAACAACGT GGAACGGGTG GGCATGGATT 37 980

GCAGGCGCTG CCCTCTACTT GCTCTGCCTG CCGGCGCTGC GTCGCGGGCT TTGGAGCGGC 38040

GCAGGGCAAC GAGCCGATCG CTGATCGTGG AAACGATAGG CCTATGCCAT GCGGGTCAAG 38100

GCGACTTCCG GCAAGCTATA CGCGCCCTAG GAGTGCGGTT GGAACGTTGG CCCAGCCAGA 38160

TACTCCCGAT CACGAGCAGG ACGCCGATGA TTTGAAGCGC ACTCAGCGTC TGATCCAAGA 38220

ACAACCATCC TAGCAACACG GCGGTCCCCG GGCTGAGAAA GCCCAGTAAG GAAACAACTG 38280

TAGGTTCGAG TCGCGAGATC CCCCGGAACC AAAGGAAGTA GGTTAAACCC GCTCCGATCA 3834 0GGCCGAGCCA CGCCAGGCCG AGAACATTGG TTCCTGTAGG CATCGGGATT GGCGGATCAA 384 00

ACACTAAAGC TACTGGAACG AGCAGAAGTC CTCCGGCCGC CAGTTGCCAG GCGGTAAAGG 384 60

TGAGCAGAGG CACGGGAGGT TGCCACTTGC GGGTCAGCAC GGTTCCGAAC GCCATGGAAA 38520

CCGCCCCCGC CAGGCCCGCT GCGACGCCGA CAGGATCTAG CGCTGCGTTT GGTGTCAACA 38580

CCAACAGCGC CACGCCCGCA GTTCCGCAAA TAGCCCCCAG GACCGCCATC AATCGTATCG 3864 0

GGCTACCTAG CAGAGCGGCA GAGATGAACA CGACCATCAG CGGCTGCACA GCGCCTACCG 387 00

TCGCCGCGAC CCCGCCCGGC AGGCGGTAGA CCGAAATAAA CAACAAGCTC CAGAATAGCG 387 60

AAATATTAAG TGCGCCGAGG ATGAAGATGC GCATCCACCA GATTCCCGTT GGAATCTGTC 38820

GGACGATCAT CACGAGCAAT AAACCCGCCG GCAACGCCCG CAGCAGCATA CCGGCGACCC 38880

CTCGGCCTCG CTGTTCGGGC TCCACGAAAA CGCCGGACAG ATGCGCCTTG TGAGCGTCCT 3894 0

TGGGGCCGTC CTCCTGTTTG AAGACCGACA GCCCAATGAT CTCGCCGTCG ATGTAGGCGC 39000

CGAATGCCAC GGCATCTCGC AACCGTTCAG CGAACGCCTC CATGGGCTTT TTCTCCTCGT 39060

GCTCGTAAAC GGACCCGAAC ATCTCTGGAG CTTTCTTCAG GGCCGACAAT CGGATCTCGC 39120

GGAAATCCTG CACGTCGGCC GCTCCAAGCC GTCGAATCTG AGCCTTAATC ACAATTGTCA 39180

ATTTTAATCC TCTGTTTATC GGCAGTTCGT AGAGCGCGCC GTGCGTCCCG AGCGATACTG 39240

AGCGAAGCAA GTGCGTCGAG CAGTGCCCGC TTGTTCCTGA AATGCCAGTA AAGCGCTGGC 39300

TGCTGAACCC CCAGCCGGAA CTGACCCCAC AAGGCCCTAG CGTTTGCAAT GCACCAGGTC 39360

ATCATTGACC CAGGCGTGTT CCACCAGGCC GCTGCCTCGC AACTCTTCGC AGGCTTCGCC 39420

GACCTGCTCG CGCCACTTCT TCACGCGGGT GGAATCCGAT CCGCACATGA GGCGGAAGGT 39480

TTCCAGCTTG AGCGGGTACG GCTCCCGGTG CGAGCTGAAA TAGTCGAACA TCCGTCGGGC 39540

CGTCGGCGAC AGCTTGCGGT ACTTCTCCCA TATGAATTTC GTGTAGTGGT CGCCAGCAAA 39600

CAGCACGACG ATTTCCTCGT CGATCAGGAC CTGGCAACGG GACGTTTTCT TGCCACGGTC 39660

CAGGACGCGG AAGCGGTGCA GCAGCGACAC CGATTCCAGG TGCCCAACGC GGTCGGACGT 39720

GAAGCCCATC GCCGTCGCCT GTAGGCGCGA CAGGCATTCC TCGGCCTTCG TGTAATACCG 3 9780

GCCATTGATC GACCAGCCCA GGTCCTGGCA AAGCTCGTAG AACGTGAAGG TGATCGGCTC 39840

GCCGATAGGG GTGCGCTTCG CGTACTCCAA CACCTGCTGC CACACCAGTT CGTCATCGTC 39900

GGCCCGCAGC TCGACGCCGG TGTAGGTGAT CTTCACGTCC TTGTTGACGT GGAAAATGAC 39960

CTTGTTTTGC AGCGCCTCGC GCGGGATTTT CTTGTTGCGC GTGGTGAACA GGGCAGAGCG 4 0020

GGCCGTGTCG TTTGGCATCG CTCGCATCGT GTCCGGCCAC GGCGCAATAT CGAACAAGGA 4 0080

AAGCTGCATT TCCTTGATCT GCTGCTTCGT GTGTTTCAGC AACGCGGCCT GCTTGGCCTC 40140

GCTGACCTGT TTTGCCAGGT CCTCGCCGGC GGTTTTTCGC TTCTTGGTCG TCATAGTTCC 4 0200

TCGCGTGTCG ATGGTCATCG ACTTCGCCAA ACCTGCCGCC TCCTGTTCGA GACGACGCGA 4 0260

ACGCTCCACG GCGGCCGATG GCGCGGGCAG GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG 4 0320

CTCGATCTTG GCCGTAGCTT GCTGGACCAT CGAGCCGACG GACTGGAAGG TTTCGCGGGG 40380

CGCACGCATG ACGGTGCGGC TTGCGATGGT TTCGGCATCC TCGGCGGAAA ACCCCGCGTC 4 0440

GATCAGTTCT TGCCTGTATG CCTTCCGGTC AAACGTCCGA TTCATTCACC CTCCTTGCGG 4 0500

GATTGCCCCG ACTCACGCCG GGGCAATGTG CCCTTATTCC TGATTTGACC CGCCTGGTGC 40560

CTTGGTGTCC AGATAATCCA CCTTATCGGC AATGAAGTCG GTCCCGTAGA CCGTCTGGCC 4 0620

GTCCTTCTCG TACTTGGTAT TCCGAATCTT GCCCTGCACG AATACCAGCG ACCCCTTGCC 4 0680

CAAATACTTG CCGTGGGCCT CGGCCTGAGA GCCAAAACAC TTGATGCGGA AGAAGTCGGT 4 0740

GCGCTCCTGC TTGTCGCCGG CATCGTTGCG CCACTCTTCA TTAACCGCTA TATCGAAAAT 40800

TGCTTGCGGC TTGTTAGAAT TGCCATGACG TACCTCGGTG TCACGGGTAA GATTACCGAT 4 0860

AAACTGGAAC TGATTATGGC TCATATCGAA AGTCTCCTTG AGAAAGGAGA CTCTAGTTTA 40920

GCTAAACATT GGTTCCGCTG TCAAGAACTT TAGCGGCTAA AATTTTGCGG GCCGCGACCA 4 0980

AAGGTGCGAG GGGCGGCTTC CGCTGTGTAC AACCAGATAT TTTTCACCAA CATCCTTCGT 4104 0

CTGCTCGATG AGCGGGGCAT GACGAAACAT GAGCTGTCGG AGAGGGCAGG GGTTTCAATT 41100

TCGTTTTTAT CAGACTTAAC CAACGGTAAG GCCAACCCCT CGTTGAAGGT GATGGAGGCC 41160

ATTGCCGACG CCCTGGAAAC TCCCCTACCT CTTCTCCTGG AGTCCACCGA CCTTGACCGC 41220

GAGGCACTCG CGGAGATTGC GGGTCATCCT TTCAAGAGCA GCGTGCCGCC CGGATACGAA 41280

CGCATCAGTG TGGTTTTGCC GTCACATAAG GCGTTTATCG TAAAGAAATG GGGCGACGAC 41340

ACCCGAAAAA AGCTGCGTGG AAGGCTCTGA CGCCAAGGGT TAGGGCTTGC ACTTCCTTCT 41400

TTAGCCGCTA AAACGGCCCC TTCTCTGCGG GCCGTCGGCT CGCGCATCAT ATCGACATCC 414 60

TCAACGGAAG CCGTGCCGCG AATGGCATCG GGCGGGTGCG CTTTGACAGT TGTTTTCTAT 41520

CAGAACCCCT ACGTCGTGCG GTTCGATTAG CTGTTTGTCT TGCAGGCTAA ACACTTTCGG 41580

TATATCGTTT GCCTGTGCGA TAATGTTGCT AATGATTTGT TGCGTAGGGG TTACTGAAAA 41640

GTGAGCGGGA AAGAAGAGTT TCAGACCATC AAGGAGCGGG CCAAGCGCAA GCTGGAACGC 41700

GACATGGGTG CGGACCTGTT GGCCGCGCTC AACGACCCGA AAACCGTTGA AGTCATGCTC 417 60

AACGCGGACG GCAAGGTGTG GCACGAACGC CTTGGCGAGC CGATGCGGTA CATCTGCGAC 41820

ATGCGGCCCA GCCAGTCGCA GGCGATTATA GAAACGGTGG CCGGATTCCA CGGCAAAGAG 41880GTCACGCGGC ATTCGCCCAT CCTGGAAGGC GAGTTCCCCT TGGATGGCAG CCGCTTTGCC 41940

GGCCAATTGC CGCCGGTCGT GGCCGCGCCA ACCTTTGCGA TCCGCAAGCG CGCGGTCGCC 4 2000

ATCTTCACGC TGGAACAGTA CGTCGAGGCG GGCATCATGA CCCGCGAGCA ATACGAGGTC 4 2060

ATTAAAAGCG CCGTCGCGGC GCATCGAAAC ATCCTCGTCA TTGGCGGTAC TGGCTCGGGC 42120

AAGACCACGC TCGTCAACGC GATCATCAAT GAAATGGTCG CCTTCAACCC GTCTGAGCGC 42180

GTCGTCATCA TCGAGGACAC CGGCGAAATC CAGTGCGCCG CAGAGAACGC CGTCCAATAC 4 2240

CACACCAGCA TCGACGTCTC GATGACGCTG CTGCTCAAGA CAACGCTGCG TATGCGCCCC 42300

GACCGCATCC TGGTCGGTGA GGTACGTGGC CCCGAAGCCC TTGATCTGTT GATGGCCTGG 42360

AACACCGGGC ATGAAGGAGG TGCCGCCACC CTGCACGCAA ACAACCCCAA AGCGGGCCTG 4 24 20

AGCCGGCTCG CCATGCTTAT CAGCATGCAC CCGGATTCAC CGAAACCCAT TGAGCCGCTG 4 2480

ATTGGCGAGG CGGTTCATGT GGTCGTCCAT ATCGCCAGGA CCCCTAGCGG CCGTCGAGTG 4 2540

CAAGAAATTC TCGAAGTTCT TGGTTACGAG AACGGCCAGT ACATCACCAA AACCCTGTAA 4 2 600

GGAGTATTTC CAATGACAAC GGCTGTTCCG TTCCGTCTGA CCATGAATCG CGGCATTTTG 42660

TTCTACCTTG CCGTGTTCTT CGTTCTCGCT CTCGCGTTAT CCGCGCATCC GGCGATGGCC 42720

TCGGAAGGCA CCGGCGGCAG CTTGCCATAT GAGAGCTGGC TGACGAACCT GCGCAACTCC 4 2780

GTAACCGGCC CGGTGGCCTT CGCGCTGTCC ATCATCGGCA TCGTCGTCGC CGGCGGCGTG 4284 0

CTGATCTTCG GCGGCGAACT CAACGCCTTC TTCCGAACCC TGATCTTCCT GGTTCTGGTG 4 2900

ATGGCGCTGC TGGTCGGCGC GCAGAACGTG ATGAGCACCT TCTTCGGTCG TGGTGCCGAA 4 2 960

ATCGCGGCCC TCGGCAACGG GGCGCTGCAC CAGGTGCAAG TCGCGGCGGC GGATGCCGTG 4 3020

CGTGCGGTAG CGGCTGGACG GCTCGCCTAA TCATGGCTCT GCGCACGATC CCCATCCGTC 4 3080

GCGCAGGCAA CCGAGAAAAC CTGTTCATGG GTGGTGATCG TGAACTGGTG ATGTTCTCGG 4 314 0

GCCTGATGGC GTTTGCGCTG ATTTTCAGCG CCCAAGAGCT GCGGGCCACC GTGGTCGGTC 4 3200

TGATCCTGTG GTTCGGGGCG CTCTATGCGT TCCGAATCAT GGCGAAGGCC GATCCGAAGA 4 3260

TGCGGTTCGT GTACCTGCGT CACCGCCGGT ACAAGCCGTA TTACCCGGCC CGCTCGACCC 4 3320

CGTTCCGCGA GAACACCAAT AGCCAAGGGA AGCAATACCG ATGATCCAAG CAATTGCGAT 4 3380

TGCAATCGCG GGCCTCGGCG CGCTTCTGTT GTTCATCCTC TTTGCCCGCA TCCGCGCGGT 4 3440

CGATGCCGAA CTGAAACTGA AAAAGCATCG TTCCAAGGAC GCCGGCCTGG CCGATCTGCT 43500

CAACTACGCC GCTGTCGTCG ATGACGGCGT AATCGTGGGC AAGAACGGCA GCTTTATGGC 4 3560

TGCCTGGCTG TACAAGGGCG ATGACAACGC AAGCAGCACC GACCAGCAGC GCGAAGTAGT 4 3620

GTCCGCCCGC ATCAACCAGG CCCTCGCGGG CCTGGGAAGT GGGTGGATGA TCCATGTGGA 4 3680

CGCCGTGCGG CGTCCTGCTC CGAACTACGC GGAGCGGGGC CTGTCGGCGT TCCCTGACCG 4 3740

TCTGACGGCA GCGATTGAAG AAGAGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT 43800

CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC 4 3860

GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA 4 3920

CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA 4 3980

GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA 4 4 040

GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT 4 4100

CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA 4 4160

TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT 4 4220

TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT 4 4280

CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA 4 4 340

GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA 44400

TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA 44 4 60

ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA 4 4520

AGCAGGGTTA TGCAGCGGAA AAGCGCTGCT TCCCTGCTGT TTTGTGGAAT ATCTACCGAC 44580

TGGAAACAGG CAAATGCAGG AAATTACTGA ACTGAGGGGA CAGGCGAGAG ACGATGCCAA 4 4 640

AGAGCTACAC CGACGAGCTG GCCGAGTGGG TTGAATCCCG CGCGGCCAAG AAGCGCCGGC 4 4700

GTGATGAGGC TGCGGTTGCG TTCCTGGCGG TGAGGGCGGA TGTCGAGGCG GCGTTAGCGT 4 47 60

CCGGCTATGC GCTCGTCACC ATTTGGGAGC ACATGCGGGA AACGGGGAAG GTCAAGTTCT 4 4 820

CCTACGAGAC GTTCCGCTCG CACGCCAGGC GGCACATCAA GGCCAAGCCC GCCGATGTGC 4 4 880

CCGCACCGCA GGCCAAGGCT GCGGAACCCG CGCCGGCACC CAAGACGCCG GAGCCACGGC 4 4 940

GGCCGAAGCA GGGGGGCAAG GCTGAAAAGC CGGCCCCCGC TGCGGCCCCG ACCGGCTTCA 45000

CCTTCAACCC AACACCGGAC AAAAAGGATC TACTGTAATG GCGAAAATTC ACATGGTTTT 4 5060

GCAGGGCAAG GGCGGGGTCG GCAAGTCGGC CATCGCCGCG ATCATTGCGC AGTACAAGAT 4 5120

GGACAAGGGG CAGACACCCT TGTGCATCGA CACCGACCCG GTGAACGCGA CGTTCGAGGG 4 5180

CTACAAGGCC CTGAACGTCC GCCGGCTGAA CATCATGGCC GGCGACGAAA TTAACTCGCG 4 5240

CAACTTCGAC ACCCTGGTCG AGCTGATTGC GCCGACCAAG GATGACGTGG TGATCGACAA 4 5300

CGGTGCCAGC TCGTTCGTGC CTCTGTCGCA TTACCTCATC AGCAACCAGG TGCCGGCTCT 4 5360

GCTGCAAGAA ATGGGGCATG AGCTGGTCAT CCATACCGTC GTCACCGGCG GCCAGGCTCT 4 5420CCTGGACACG GTGAGCGGCT TCGCCCAGCT CGCCAGCCAG TTCCCGGCCG AAGCGCTTTT 45480

CGTGGTCTGG CTGAACCCGT ATTGGGGGCC TATCGAGCAT GAGGGCAAGA GCTTTGAGCA 45540

GATGAAGGCG TACACGGCCA ACAAGGCCCG CGTGTCGTCC ATCATCCAGA TTCCGGCCCT 45600

CAAGGAAGAA ACCTACGGCC GCGATTTCAG CGACATGCTG CAAGAGCGGC TGACGTTCGA 45660

CCAGGCGCTG GCCGATGAAT CGCTCACGAT CATGACGCGG CAACGCCTCA AGATCGTGCG 45720

GCGCGGCCTG TTTGAACAGC TCGACGCGGC GGCCGTGCTA TGAGCGACCA GATTGAAGAG 45780

CTGATCCGGG AGATTGCGGC CAAGCACGGC ATCGCCGTCG GCCGCGACGA CCCGGTGCTG 45840

ATCCTGCATA CCATCAACGC CCGGCTCATG GCCGACAGTG CGGCCAAGCA AGAGGAAATC 45900

CTTGCCGCGT TCAAGGAAGA GCTGGAAGGG ATCGCCCATC GTTGGGGCGA GGACGCCAAG 45960

GCCAAAGCGG AGCGGATGCT GAACGCGGCC CTGGCGGCCA GCAAGGACGC AATGGCGAAG 46020

GTAATGAAGG ACAGCGCCGC GCAGGCGGCC GAAGCGATCC GCAGGGAAAT CGACGACGGC 46080

CTTGGCCGCC AGCTCGCGGC CAAGGTCGCG GACGCGCGGC GCGTGGCGAT GATGAACATG 46140

ATCGCCGGCG GCATGGTGTT GTTCGCGGCC GCCCTGGTGG TGTGGGCCTC GTTATGAATC 46200

GCAGAGGCGC AGATGAAAAA GCCCGGCGTT GCCGGGCTTT GTTTTTGCGT TAGCTGGGCT 46260

TGTTTGACAG GCCCAAGCTC TGACTGCGCC CGCGCTCGCG CTCCTGGGCC TGTTTCTTCT 46320

CCTGCTCCTG CTTGCGCATC AGGGCCTGGT GCCGTCGGGC TGCTTCACGC ATCGAATCCC 46380

AGTCGCCGGC CAGCTCGGGA TGCTCCGCGC GCATCTTGCG CGTCGCCAGT TCCTCGATCT 46440

TGGGCGCGTG AATGCCCATG CCTTCCTTGA TTTCGCGCAC CATGTCCAGC CGCGTGTGCA 46500

GGGTCTGCAA GCGGGCTTGC TGTTGGGCCT GCTGCTGCTG CCAGGCGGCC TTTGTACGCG 46560

GCAGGGACAG CAAGCCGGGG GCATTGGACT GTAGCTGCTG CAAACGCGCC TGCTGACGGT 46620

CTACGAGCTG TTCTAGGCGG TCCTCGATGC GCTCCACCTG GTCATGCTTT GCCTGCACGT 46680

AGAGCGCAAG GGTCTGCTGG TAGGTCTGCT CGATGGGCGC GGATTCTAAG AGGGCCTGCT 46740

GTTCCGTCTC GGCCTCCTGG GCCGCCTGTA GCAAATCCTC GCCGCTGTTG CCGCTGGACT 46800

GCTTTACTGC CGGGGACTGC TGTTGCCCTG CTCGCGCCGT CGTCGCAGTT CGGCTTGCCC 46860

CCACTCGATT GACTGCTTCA TTTCGAGCCG CAGCGATGCG ATCTCGGATT GCGTCAACGG 46920

ACGGGGCAGC GCGGAGGTGT CCGGCTTCTC CTTGGGTGAG TCGGTCGATG CCATAGCCAA 46980

AGGTTTCCTT CCAAAATGCG TCCATTGCTG GACCGTGTTT CTCATTGATG CCCGCAAGCA 47040

TCTTCGGCTT GACCGCCAGG TCAAGCGCGC CTTCATGGGC GGTCATGACG GACGCCGCCA 47100

TGACCTTGCC GCCGTTGTTC TCGATGTAGC CGCGTAATGA GGCAATGGTG CCGCCCATCG 47160

TCAGCGTGTC ATCGACAACG ATGTACTTCT GGCCGGGGAT CACCTCCCCC TCGAAAGTCG 47220

GGTTGAACGC CAGGCGATGA TCTGAACCGG CTCCGGTTCG GGCGACCTTC TCCCGCTGCA 47280

CAATGTCCGT TTCGACCTCA AGGCCAAGGC GGTCGGCCAG AACGACCGCC ATCATGGCCG 47340

GAATCTTGTT GTTCCCCGCC GCCTCGACGG CGAGGACTGG AACGATGCGG GGCTTGTCGT 47400

CGCCGATCAG CGTCTTGAGC TGGGCAACAG TGTCGTCCGA AATCAGGCGC TCGACCAAAT 47460

TAAGCGCCGC TTCCGCGTCG CCCTGCTTCG CAGCCTGGTA TTCAGGCTCG TTGGTCAAAG 47520

AACCAAGGTC GCCGTTGCGA ACCACCTTCG GGAAGTCTCC CCACGGTGCG CGCTCGGCTC 47580

TGCTGTAGCT GCTCAAGACG CCTCCCTTTT TAGCCGCTAA AACTCTAACG AGTGCGCCCG 47640

CGACTCAACT TGACGCTTTC GGCACTTACC TGTGCCTTGC CACTTGCGTC ATAGGTGATG 47700

CTTTTCGCAC TCCCGATTTC AGGTACTTTA TCGAAATCTG ACCGGGCGTG CATTACAAAG 47760

TTCTTCCCCA CCTGTTGGTA AATGCTGCCG CTATCTGCGT GGACGATGCT GCCGTCGTGG 47820

CGCTGCGACT TATCGGCCTT TTGGGCCATA TAGATGTTGT AAATGCCAGG TTTCAGGGCC 47880

CCGGCTTTAT CTACCTTCTG GTTCGTCCAT GCGCCTTGGT TCTCGGTCTG GACAATTCTT 47940

TGCCCATTCA TGACCAGGAG GCGGTGTTTC ATTGGGTGAC TCCTGACGGT TGCCTCTGGT 48000

GTTAAACGTG TCCTGGTCGC TTGCCGGCTA AAAAAAAGCC GACCTCGGCA GTTCGAGGCC 48060

GGCTTTCCCT AGAGCCGGGC GCGTCAAGGT TGTTCCATCT ATTTTAGTGA ACTGCGTTCG 48120

ATTTATCAGT TACTTTCCTC CCGCTTTGTG TTTCCTCCCA CTCGTTTCCG CGTCTAGCCG 48180

ACCCCTCAAC ATAGCGGCCT CTTCTTGGGC TGCCTTTGCC TCTTGCCGCG CTTCGTCACG 48240

CTCGGCTTGC ACCGTCGTAA AGCGCTCGGC CTGCCTGGCC GCCTCTTGCG CCGCCAACTT 48300

CCTTTGCTCC TGGTGGGCCT CGGCGTCGGC CTGCGCCTTC GCTTTCACCG CTGCCAACTC 48360

CGTGCGCAAA CTCTCCGCTT CGCGCCTGGT GGCGTCGCGC TCGCCGCGAA GCGCCTGCAT 48420

TTCCTGGTTG GCCGCGTCCA GGGTCTTGCG GCTCTCTTCT TTGAATGCGC GGGCGTCCTG 48480

GTGAGCGTAG TCCAGCTCGG CGCGCAGCTC CTGCGCTCGA CGCTCCACCT CGTCGGCCCG 48540

CTGCGTCGCC AGCGCGGCCC GCTGCTCGGC TCCTGCCAGG GCGGTGCGTG CTTCGGCCAG 48600

GGCTTGCCGC TGGCGTGCGG CCAGCTCGGC CGCCTCGGCG GCCTGCTGCT CTAGCAATGT 48660

AACGCGCGCC TGGGCTTCTT CCAGCTCGCG GGCCTGCGCC TCGAAGGCGT CGGCCAGCTC 48720

CCCGCGCACG GCTTCCAACT CGTTGCGCTC ACGATCCCAG CCGGCTTGCG CTGCCTGCAA 48780

CGATTCATTG GCAAGGGCCT GGGCGGCTTG CCAGAGGGCG GCCACGGCCT GGTTGCCGGC 48840

CTGCTGCACC GCGTCCGGCA CCTGGACTGC CAGCGGGGCG GCCTGCGCCG TGCGCTGGCG 48900

TCGCCATTCG CGCATGCCGG CGCTGGCGTC GTTCATGTTG ACGCGGGCGG CCTTACGCAC 48960TGCATCCACG GTCGGGAAGT TCTCCCGGTC GCCTTGCTCG AACAGCTCGT CCGCAGCCGC 4 9020

AAAAATGCGG TCGCGCGTCT CTTTGTTCAG TTCCATGTTG GCTCCGGTAA TTGGTAAGAA 4 9080

TAATAATACT CTTACCTACC TTATCAGCGC AAGAGTTTAG CTGAACAGTT CTCGACTTAA 4 9140

CGGCAGGTTT TTTAGCGGCT GAAGGGCAGG CAAAAAAAGC CCCGCACGGT CGGCGGGGGC 4 9200

AAAGGGTCAG CGGGAAGGGG ATTAGCGGGC GTCGGGCTTC TTCATGCGTC GGGGCCGCGC 4 9260

TTCTTGGGAT GGAGCACGAC GAAGCGCGCA CGCGCATCGT CCTCGGCCCT ATCGGCCCGC 4 9320

GTCGCGGTCA GGAACTTGTC GCGCGCTAGG TCCTCCCTGG TGGGCACCAG GGGCATGAAC 4 9380

TCGGCCTGCT CGATGTAGGT CCACTCCATG ACCGCATCGC AGTCGAGGCC GCGTTCCTTC 4 9440

ACCGTCTCTT GCAGGTCGCG GTACGCCCGC TCGTTGAGCG GCTGGTAACG GGCCAATTGG 4 9500

TCGTAAATGG CTGTCGGCCA TGAGCGGCCT TTCCTGTTGA GCCAGCAGCC GACGACGAAG 4 9560

CCGGCAATGC AGGCCCCTGG CACAACCAGG CCGACGCCGG GGGCAGGGGA TGGCAGCAGC 4 9620

TCGCCAACCA GGAACCCCGC CGCGATGATG CCGATGCCGG TCAACCAGCC CTTGAAACTA 4 9680

TCCGGCCCCG AAACACCCCT GCGCATTGCC TGGATGCTGC GCCGGATAGC TTGCAACATC 4 9740

AGGAGCCGTT TCTTTTGTTC GTCAGTCATG GTCCGCCCTC ACCAGTTGTT CGTATCGGTG 4 9800

TCGGACGAAC TGAAATCGCA AGAGCTGCCG GTATCGGTCC AGCCGCTGTC CGTGTCGCTG 4 98 60

CTGCCGAAGC ACGGCGAGGG GTCCGCGAAC GCCGCAGACG GCGTATCCGG CCGCAGCGCA 4 9920

TCGCCCAGCA TGGCCCCGGT CAGCGAGCCG CCGGCCAGGT AGCCCAGCAT GGTGCTGTTG 4 9980

GTCGCCCCGG CCACCAGGGC CGACGTGACG AAATCGCCGT CATTCCCTCT GGATTGTTCG 5004 0

CTGCTCGGCG GGGCAGTGCG CCGCGCCGGC GGCGTCGTGG ATGGCTCGGG TTGGCTGGCC 50100

TGCGACGGCC GGCGAAAGGT GCGCAGCAGC TCGTTATCGA CCGGCTGCGG CGTCGGGGCC 50160

GCCGCCTTGC GCTGCGGTCG GTGTTCCTTC TTCGGCTCGC GCAGCTTGAA CAGCATGATC 50220

GCGGAAACCA GCAGCAACGC CGCGCCTACG CCTCCCGCGA TGTAGAACAG CATCGGATTC 50280

ATTCTTCGGT CCTCCTTGTA GCGGAACCGT TGTCTGTGCG GCGCGGGTGG CCCGCGCCGC 5034 0

TGTCTTTGGG GATCAGCCCT CGATGAGCGC GACCAGTTTC ACGTCGGCAA GGTTCGCCTC 50400

GAACTCCTGG CCGTCGTCCT CGTACTTCAA CCAGGCATAG CCTTCCGCCG GCGGCCGACG 504 60

GTTGAGGATA AGGCGGGCAG GGCGCTCGTC GTGCTCGACC TGGACGATGG CCTTTTTCAG 50520

CTTGTCCGGG TCCGGCTCCT TCGCGCCCTT TTCCTTGGCG TCCTTACCGT CCTGGTCGCC 50580

GTCCTCGCCG TCCTGGCCGT CGCCGGCCTC CGCGTCACGC TCGGCATCAG TCTGGCCGTT 50640

GAAGGCATCG ACGGTGTTGG GATCGCGGCC CTTCTCGTCC AGGAACTCGC GCAGCAGCTT 50700

GACCGTGCCG CGCGTGATTT CCTGGGTGTC GTCGTCAAGC CACGCCTCGA CTTCCTCCGG 507 60

GCGCTTCTTG AAGGCCGTCA CCAGCTCGTT CACCACGGTC ACGTCGCGCA CGCGGCCGGT 50820

GTTGAACGCA TCGGCGATCT TCTCCGGCAG GTCCAGCAGC GTGACGTGCT GGGTGATGAA 50880

CGCCGGCGAC TTGCCGATTT CCTTGGCGAT ATCGCCTTTC TTCTTGCCCT TCGCCAGCTC 5094 0

GCGGCCAATG AAGTCGGCAA TTTCGCGCGG GGTCAGCTCG TTGCGTTGCA GGTTCTCGAT 51000

AACCTGGTCG GCTTCGTTGT AGTCGTTGTC GATGAACGCC GGGATGGACT TCTTGCCGGC 51060

CCACTTCGAG CCACGGTAGC GGCGGGCGCC GTGATTGATG ATATAGCGGC CCGGCTGCTC 51120

CTGGTTCTCG CGCACCGAAA TGGGTGACTT CACCCCGCGC TCTTTGATCG TGGCACCGAT 51180

TTCCGCGATG CTCTCCGGGG AAAAGCCGGG GTTGTCGGCC GTCCGCGGCT GATGCGGATC 5124 0

TTCGTCGATC AGGTCCAGGT CCAGCTCGAT AGGGCCGGAA CCGCCCTGAG ACGCCGCAGG 51300

AGCGTCCAGG AGGCTCGACA GGTCGCCGAT GCTATCCAAC CCCAGGCCGG ACGGCTGCGC 51360

CGCGCCTGCG GCTTCCTGAG CGGCCGCAGC GGTGTTTTTC TTGGTGGTCT TGGCTTGAGC 51420

CGCAGTCATT GGGAAATCTC CATCTTCGTG AACACGTAAT CAGCCAGGGC GCGAACCTCT 51480

TTCGATGCCT TGCGCGCGGC CGTTTTCTTG ATCTTCCAGA CCGGCACACC GGATGCGAGG 5154 0

GCATCGGCGA TGCTGCTGCG CAGGCCAACG GTGGCCGGAA TCATCATCTT GGGGTACGCG 51600

GCCAGCAGCT CGGCTTGGTG GCGCGCGTGG CGCGGATTCC GCGCATCGAC CTTGCTGGGC 51660

ACCATGCCAA GGAATTGCAG CTTGGCGTTC TTCTGGCGCA CGTTCGCAAT GGTCGTGACC 51720

ATCTTCTTGA TGCCCTGGAT GCTGTACGCC TCAAGCTCGA TGGGGGACAG CACATAGTCG 517 80

GCCGCGAAGA GGGCGGCCGC CAGGCCGACG CCAAGGGTCG GGGCCGTGTC GATCAGGCAC 5184 0

ACGTCGAAGC CTTGGTTCGC CAGGGCCTTG ATGTTCGCCC CGAACAGCTC GCGGGCGTCG 51900

TCCAGCGACA GCCGTTCGGC GTTCGCCAGT ACCGGGTTGG ACTCGATGAG GGCGAGGCGC 51960

GCGGCCTGGC CGTCGCCGGC TGCGGGTGCG GTTTCGGTCC AGCCGCCGGC AGGGACAGCG 52020

CCGAACAGCT TGCTTGCATG CAGGCCGGTA GCAAAGTCCT TGAGCGTGTA GGACGCATTG 52080

CCCTGGGGGT CCAGGTCGAT CACGGCAACC CGCAAGCCGC GCTCGAAAAA GTCGAAGGCA 52140

AGATGCACAA GGGTCGAAGT CTTGCCGACG CCGCCTTTCT GGTTGGCCGT GACCAAAGTT 52200

TTCATCGTTT GGTTTCCTGT TTTTTCTTGG CGTCCGCTTC CCACTTCCGG ACGATGTACG 52260

CCTGATGTTC CGGCAGAACC GCCGTTACCC GCGCGTACCC CTCGGGCAAG TTCTTGTCCT 52320

CGAACGCGGC CCACACGCGA TGCACCGCTT GCGACACTGC GCCCCTGGTC AGTCCCAGCG 52380

ACGTTGCGAA CGTCGCCTGT GGCTTCCCAT CGACTAAGAC GCCCCGCGCT ATCTCGATGG 52440

TCTGCTGCCC CACTTCCAGC CCCTGGATCG CCTCCTGGAA CTGGCTTTCG GTAAGCCGTT 52500TCTTCATGGA TAACACCCAT AATTTGCTCC GCGCCTTGGT TGAACATAGC GGTGACAGCC 52560

GCCAGCACAT GAGAGAAGTT TAGCTAAACA TTTCTCGCAC GTCAACACCT TTAGCCGCTA 52620

AAACTCGTCC TTGGCGTAAC AAAACAAAAG CCCGGAAACC GGGCTTTCGT CTCTTGCCGC 52 680

TTATGGCTCT GCACCCGGCT CCATCACCAA CAGGTCGCGC ACGCGCTTCA CTCGGTTGCG 52740

GATCGACACT GCCAGCCCAA CAAAGCCGGT TGCCGCCGCC GCCAGGATCG CGCCGATGAT 52800

GCCGGCCACA CCGGCCATCG CCCACCAGGT CGCCGCCTTC CGGTTCCATT CCTGCTGGTA 52860

CTGCTTCGCA ATGCTGGACC TCGGCTCACC ATAGGCTGAC CGCTCGATGG CGTATGCCGC 52 920

TTCTCCCCTT GGCGTAAAAC CCAGCGCCGC AGGCGGCATT GCCATGCTGC CCGCCGCTTT 52 980

CCCGACCACG ACGCGCGCAC CAGGCTTGCG GTCCAGACCT TCGGCCACGG CGAGCTGCGC 5304 0

AAGGACATAA TCAGCCGCCG ACTTGGCTCC ACGCGCCTCG ATCAGCTCTT GCACTCGCGC 53100

GAAATCCTTG GCCTCCACGG CCGCCATGAA TCGCGCACGC GGCGAAGGCT CCGCAGGGCC 53160

GGCGTCGTGA TCGCCGCCGA GAATGCCCTT CACCAAGTTC GACGACACGA AAATCATGCT 53220

GACGGCTATC ACCATCATGC AGACGGATCG CACGAACCCG CTGAATTGAA CACGAGCACG 53280

GCACCCGCGA CCACTATGCC AAGAATGCCC AAGGTAAAAA TTGCCGGCCC CGCCATGAAG 53340

TCCGTGAATG CCCCGACGGC CGAAGTGAAG GGCAGGCCGC CACCCAGGCC GCCGCCCTCA 534 00

CTGCCCGGCA CCTGGTCGCT GAATGTCGAT GCCAGCACCT GCGGCACGTC AATGCTTCCG 534 60

GGCGTCGCGC TCGGGCTGAT CGCCCATCCC GTTACTGCCC CGATCCCGGC AATGGCAAGG 53520

ACTGCCAGCG CTGCCATTTT TGGGGTGAGG CCGTTCGCGG CCGAGGGGCG CAGCCCCTGG 53580

GGGGATGGGA GGCCCGCGTT AGCGGGCCGG GAGGGTTCGA GAAGGGGGGG CACCCCCCTT 53 640

CGGCGTGCGC GGTCACGCGC ACAGGGCGCA GCCCTGGTTA AAAACAAGGT TTATAAATAT 53700

TGGTTTAAAA GCAGGTTAAA AGACAGGTTA GCGGTGGCCG AAAAACGGGC GGAAACCCTT 537 60

GCAAATGCTG GATTTTCTGC CTGTGGACAG CCCCTCAAAT GTCAATAGGT GCGCCCCTCA 53820

TCTGTCAGCA CTCTGCCCCT CAAGTGTCAA GGATCGCGCC CCTCATCTGT CAGTAGTCGC 53880

GCCCCTCAAG TGTCAATACC GCAGGGCACT TATCCCCAGG CTTGTCCACA TCATCTGTGG 53940

GAAACTCGCG TAAAATCAGG CGTTTTCGCC GATTTGCGAG GCTGGCCAGC TCCACGTCGC 54 000

CGGCCGAAAT CGAGCCTGCC CCTCATCTGT CAACGCCGCG CCGGGTGAGT CGGCCCCTCA 54 060

AGTGTCAACG TCCGCCCCTC ATCTGTCAGT GAGGGCCAAG TTTTCCGCGA GGTATCCACA 54120

ACGCCGGCGG CCGCGGTGTC TCGCACACGG CTTCGACGGC GTTTCTGGCG CGTTTGCAGG 54180

GCCATAGACG GCCGCCAGCC CAGCGGCGAG GGCAACCAGC CCGGTGAGCG TCGGAAAGGC 54 24 0

GCTGGAAGCC CCGTAGCGAC GCGGAGAGGG GCGAGACAAG CCAAGGGCGC AGGCTCGATG 54300

CGCAGCACGA CATAGCCGGT TCTCGCAAGG ACGAGAATTT CCCTGCGGTG CCCCTCAAGT 54360

GTCAATGAAA GTTTCCAACG CGAGCCATTC GCGAGAGCCT TGAGTCCACG CTAGATGAGA 54 4 20

GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT 54 4 80

GCGTTGTCGG GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA 54 54 0

CAAAGCCACG TTGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA 54600

TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT 54 660

ATTCAACGGG AAACGTCTTG CTCGAC 54 686

<210> 89<211> 3324<212> DNA<213> Zea mays

<4 00> 89

TGGTCCTTGT TTGATTTACT TCCAGGATTA TATAATCCAG CTTATGGATT ATATAAGTAC 60

CTATTGACGT CACGTGCTTA TGTATTATAA TAATCTAGGT ATATAGATTA TATAATCTAT 120

CTAATAATAA TCTGTGTTGT TTGTTTATCT CTCAAAACAA ACAGGTCCTA AAATGGTCCC 180

GGGCGTCCAA TGTGTCGTCA AGTAGTGTTA AGCTAAATCG ACATTTCTTT GTGGGTTGTG 24 0

TGGAAGGTGT TCCTTTTCCT TAAGTTGTTA GTTGTGCAAG GTGTTCCTTA GAGCATCTCC 300

AATAGGACCT ATAATGGATT CTATTTTGAA TTATAAGACT CTAACAACAA AAGCATACTT 360

TAATGGGGAT TCTATTTTAC ΑΑΑΑΑΑΑΤΑΤ CAAATGATTA TATGGTCGAT TCCTCGGGTC 4 20

CTAAATATAG TATCTCATAT AATAGAGCTC TATCCTCATT TTATATACTA TTTTTAAGTT 4 80

TTTATTTACT AAATAACATG ATTTATTTTC TAATACTATG AACTCAACTA TTAGAGCTGT 540

AAACGTTTTT GTGGTACTAA ACACTTTAAA TCAGGTCCTA TTTTAATTTG AAGGACTTAA 600

ATATAAGACT TCTGGTTAGA GATGCTCTTA GCGAGTGTTT GTGCATGATT GCTATTTAGT 660

CTTTGTGGAT TGTGGAAGGT GTTACTTTTC CTCAAGTTGT TAGTTGTGCA AGGTGTTTCT 720

TAGAGCATCT CTAACAGGAG CCTTAACGGA ATCTATTTTG AAGTATAGTA CTTTAACACC 780

AAAAACATAC TTTAATAGGG GTCCTATTTT ACAAAAAAAT TATCAAATGA TTATAAGGTC 84 0

CACTCCTCGG GTCCTAAATA TAATATCTCA TATACTAGAG CTCTATCCTC ΑΤΤΤΤΑΤΑΤΑ 900CTATCCCTAG GTTTTTATTC CCTAAATAAC ATGATTTATT TCCTAATACT AAGATATAGG 960

GCTCAACTAT TGGAGTTGCA AATGTTTTTT GGCACTAAAC ACTTTATATC AGGTCCTATT 1020

TTAATTTTAA TTTGAAGGAC TCAAATATAG GACTTCTCGT TAGAGATGCT CTTAGCGAGT 1080

GTTTGTGCAT GATTGCTATT TATGTCTGTA GTTTAGTTGG GGGCTTTAAT ATGTTTAGTT 1140

GAAGTTCTAG TATTTTTTAG GTTCTCCACT CTTTGGATTA TGACAACGAC CACTATCCAA 1200

GCAGTCTTTG AGTGCAAACG CGCGAGCAAA CTATCTGATC TATTAAATTA TGATCCAACC 1260

GTTATGTCAT ATTGAAGACT TAAACCCTTT CACCACCAGC CCAAGTATCT TTATGAAAAA 1320

CCCTAACAAA CCACAATTGC ATCTATGGTT GGATTATAAT TTAACGTATC AGATGGTTCG 1380

CTTGCATGCT TACATATCTA GAAACTGTTT GCATAACAGT CGTTCTCTTT GGTTATATAA 14 40

TGCTTTAGTA ATCATCAGCC AAGTGTAAAC AAATGGTACA AACTAGTAGT GAACACATCC 1500

TCCCTACCTA TCTCTAGGGG TGTCATAGTA AATTCTATGT CTTATTTGTC CGCGATTGAA 1560

GAAAAATGAC AAAAAGATCT GACATTCGAA TAAACATCTG TTTCCACTCC TACCTATCTG 1620

ACCTCCTATT TCAAACTCCA CTTTGTAACA CGGTACAAAA TCACTCCCTA CCTATCTGAC 1680

CTCCTATTTC AAACTCCACT CAGTAAACAA TATTGTCTAT GGTACAAAAC CAAGTGTTTT 17 4 0

ATACATCTAT TTGCACGATC TGCTCGAGTC AGGCATCCTT GACACACAAC ATACTCCTTG 1800

TGGCTATAAA TGTCCAAATA GAGCAGACCT AATGGGTGGA CCGTTGCATG ACACGACTTA 18 60

TCCCAAGACG AGCACAGTTC GCCCCATTGG TCATGGGGGT CCGGGCTAGT CTAGCCTGAT 1920

CATCGGGTCA CACTTAGGCC ACAGGTGTGC CACAACGGGA TAGCCCAACA TGTCCCTTTT 1980

TGTCATGCAT ATATCTATAT TATAGTTAGT ATAATGTAAA AAAACAAAAG GTATGTGTGT 204 0

TATGTTGGTT AGATGTGTTT AAATAACTCT TTAAAGCTAG CAACTATGGT TTAAATCATA 2100

CATATACACA TTTTTATTTT ATTTTTATTT AAACGATATG GGCCTTCTAG GCACGTCGAG 2160

TGTGACGGGC CAGTGAGATG ACACATTATA ATTACTGGTC TAGCAGGCCG TACCTAGGTC 2220

TTTCTCGTGG GCCAAGACTA AGGGTTGGCC CGTTGGCTAA TCTGTACGGT ACCGATACTG 2280

TCCTAATTCA TTTGAACACC TGTAGAAGAG GGGAATTTAT AATTGAGGAG GAATGTACTC 234 0

ATGCGGTACA CCAGGGGAAT TGTTTTGTTG TGCTCAGCGA TAGATTTCAA CGCAACGGTG 24 00

AGCCAGTTTC ACTAAAAAAA GGGGGGGGGG GGGGGGGGGG GGAAGGCCAC ATCAAAGGCG 24 60

AGGTGCTGAC GAGCAGAAGA TGCTAGCAGT GACGCCAAGT CCAGCAGCTA GCAATGAAAG 2520

GGTACTCGGG ATTTAACAAT GCCTAGAGAC GGCATCATCC CCTCAATAAT CCGGTGCTCT 2580

CTTTTTGTTT ATTCACCAGT TGGCGTAGCT ATATACACAT GTCTGGTCTG ACGAACAAAT 2 64 0

CAAGGGATCG CTAGCTCGGG CTAGCCTTCC TATCACTGTC ATGACATGTG CTCTGCCTCT 2700

GCTGGTTGAT AAGCCGTGCG CCTTCTCGCT AATTCTTTCT TGTGCTAGAG GCGAGTCAAA 27 60

CAAACGCTGC ACCTCGTAGC CCTTAATCTG CGCTAAGGGT CACATGACCC TGTTCCCTAT 2820

CGCTAGTTAC CAACGACCCA TTCCCCCTGA CAGATACTTA CGACGCGTCC GTACGCGGCA 2880

GGCCTCGGCA GTTCGGCATC ACCAGCACCG GCGCCGGCAT TCGCCCCCTG CCAGCCGGTT 2 94 0

CGCAGATTCG CAGGGCGGAG TCGGCCGCAG TTGCCGCATC CCAAACGCCC GGGAACCTTT 3000

GGGGCCCCTC TACGAGCAAA TGAAGTTGCT GCCCCTGGCT TCGTAAAGCT CTGACTTTTG 3060

ATCACTTGAT TGGCAGTCGT ACTCCTCGCT CATAGGCCGA CACGGCCGCA AAGTCAACTA 3120

CCCGCTCCGC CATCCTTCAA CCCCCGCCAC GCGCCTATAT ATGTTCGCGG CCATGTCCGT 3180

ACTAGTCCTC CAACCCACAA GCCACAACCC CGAGCTCAGA TCCCTCGCCT CGTGTCGTGT 3240

CTCCGGTCGA CGACGACCAA CAGCCAGTGT GGGCCAGACG GACACCGCCG AGCTATAGCG 3300

CTTGGTGATA GCAAGGGACG ACCG 3324

<210> 90<211> 500<212> DNA<213> Zea mays

<4 00> 90

AGTTACCAAC GACCCATTCC CCCTGACAGA TACTTACGAC GCGTCCGTAC GCGGCAGGCC 60

TCGGCAGTTC GGCATCACCA GCACCGGCGC CGGCATTCGC CCCCTGCCAG CCGGTTCGCA 120

GATTCGCAGG GCGGAGTCGG CCGCAGTTGC CGCATCCCAA ACGCCCGGGA ACCTTTGGGG 180

CCCCTCTACG AGCAAATGAA GTTGCTGCCC CTGGCTTCGT AAAGCTCTGA CTTTTGATCA 240

CTTGATTGGC AGTCGTACTC CTCGCTCATA GGCCGACACG GCCGCAAAGT CAACTACCCG 300

CTCCGCCATC CTTCAACCCC CGCCACGCGC CTATATATGT TCGCGGCCAT GTCCGTACTA 360

GTCCTCCAAC CCACAAGCCA CAACCCCGAG CTCAGATCCC TCGCCTCGTG TCGTGTCTCC 420

GGTCGACGAC GACCAACAGC CAGTGTGGGC CAGACGGACA CCGCCGAGCT ATAGCGCTTG 4 80

GTGATAGCAA GGGACGACCG 500<210> 91<211> 2025<212> DNA<213> Zea mays

<400> 91

GAGCGCTCCG CTGCCGTGCG CGCCCCCGCG CCGGCCTCCC ACTGGATCGC TCCACCTCAT 60

GCTCCAAATC TTTATTGGTT TCCACGTTGC CCCCTCGCCG TCCCCAACCA TCGACCGCGC 120

CGCGCCCGCT GCCGCCTCCC AGCTCGCTCT ATATAAACAC CACGTACGCG CCGAAGCATC 180

AGCACAGCCA CGTACGTACG ACCGGCTTCC GGCAGGTGAG AGAACAGTGA GAAGCAGGCG 240

AGCGGTGACA TGGCGGAGGG GGAGTTCAAG CCCGCGGCGA TGCAGGTGGA GGCTCCTGCC 300

GAGGCGGCGG CGGCGCCGTC CAAGCCGCGG TTCAGGATGC CCGTCGACTC CGACAACAAG 360

GCCACCGAGT TCTGGCTCTT CTCCTTCGCG AGGCCGCACA TGAGCGCCTT CCACATGTCG 420

TGGTTCTCCT TCTTCTGCTG CTTCCTCTCC ACCTTCGCGG CGCCGCCGCT GCTCCCGCTC 480

ATCCGGGACA CGCTGGGGCT CACGGCCACG GACATCGGCA ACGCCGGGAT CGCCTCCGTG 540

TCCGGCGCGG TCTTCGCGCG CGTGGCCATG GGCACGGCGT GCGACCTGGT GGGCCCGCGC 600

CTGGCGTCCG CGGCCATCAT ACTCCTCACC ACGCCCGCCG TCTACTACTC CGCCGTCATC 660

GACTCCGCCT CGTCCTACCT GCTCGTGCGC TTCTTCACGG GCTTCTCGCT CGCGTCCTTC 720

GTGTCCACGC AGTTCTGGAT GAGCTCCATG TTCTCGCCGC CCAAGGTGGG GCTGGCCAAC 7 80

GGCGTCGCCG GGGGGTGGGG CAACCTCGGC GGCGGCGCCG TGCAGCTCAT CATGCCGCTC 84 0

GTGTTCGAGG CCATCCGCAA GGCCGGGGCC ACGCCGTTCA CGGCGTGGCG CGTCGCCTTC 900

TTCGTCCCGG GCCTGCTGCA GACGCTGTCG GCCGTCGCCG TGCTGGCGTT CGGCCAGGAC 960

ATGCCCGACG GCAACTACCG CAAGCTGCAC AGGTCCGGCG ACATGCACAA GGACAGCTTC 1020

GGCAACGTGC TCCGCCACGC CGTCACCAAC TACCGCGCCT GGATCCTGGC GCTCACCTAC 1080

GGATACTGCT TCGGCGTGGA GCTCGCCGTG GACAACATCG TCGCGCAGTA CTTCTACGAC 114 0

CGCTTCGGCG TCAAGCTCAG CACCGCCGGC TTCATCGCCG CCAGCTTCGG GATGGCCAAC 1200

ATCGTCTCCC GCCCCGGCGG CGGCCTCCTG TCGGACTGGC TCTCCAGCCG CTTCGGCATG 1260

CGCGGCAGGC TGTGGGGCCT GTGGGTGGTG CAGACCATCG GGGGCGTCCT CTGCGTCGTG 1320

CTCGGCGCCG TCGACTACTC CTTCGCCGCG TCCGTGGCCG TCATGATACT CTTCTCCATG 1380

TTCGTGCAGG CGGCCTGCGG GCTCACCTTT GGCATCGTCC CGTTCGTCTC CCGAAGGTCG 1440

CTGGGGCTCA TCTCCGGCAT GACCGGCGGC GGCGGCAACG TGGGCGCCGT GCTCACGCAG 1500

CTCATCTTCT TCCACGGATC CAAGTACAAG ACGGAGACGG GGATCAAGTA CATGGGGTTC 1560

ATGATCATCG CCTGCACGTT GCCCATCACG CTCATCTACT TCCCGCAGTG GGGCGGCATG 1620

TTCCTGGGGC CGCGGCCCGG GGCGACGGCG GAGGACTACT ACAACCGGGA GTGGACAGCG 1680

CACGAGTGCG ACAAGGGTTT CAACACCGCG AGCGTACGCT TTGCGGAGAA CAGCGTGCGG 1740

GAAGGGGGAC GCTCGGGCAG CCAGTCCAAG CACACTACTG TGCCCGTCGA GTCCTCGCCG 1800

GCCGACGTGT GAAACACACA CAAGCATACG GTACTGCCCG TATAATCAGC GGTCCCTCCC 1860

GTGTCAGCAA ATCATATGTA GTGTTCCTAA GTCGTGATGA CTCCGTACGT GTGGTAATTT 1920

CTGTGTGAAG GAAAAACCGG GGGTGAATTT CAGCGAGGAG TGACATTATA AGCAGGGCTC 1980

GTTTGCATAA ΑΑΑΑΑΑΑΑΑΑ ΑΑΑΑΑΑΑΑΑΑ ΑΑΑΑΑΑΑΑΑΑ AAAAA 2025

<210> 92<211> 520<212> PRT<213> Zea mays

<4 00> 92

MET ALA GLU GLY GLU PHE LYS PRO ALA ALA MET GLN VAL GLU ALA PRO15 10 15

ALA GLU ALA ALA ALA ALA PRO SER LYS PRO ARG PHE ARG MET PRO VAL

20

25

30

ASP SER ASP ASN LYS ALA THR GLU PHE TRP

35

40

LEU PHE SER PHE ALA ARG45

PRO HIS MET50

SER ALA PHE HIS MET SER TRP55

PHE SER PHE PHE CYS CYS60PHE LEU SER THR PHE ALA ALA PRO PRO LEU LEU PRO LEU ILE ARG ASP65 70 75 80

THR LEU GLY LEU THR ALA THR ASP ILE GLY ASN ALA GLY ILE ALA SER85 90 95

VAL SER GLY ALA VAL PHE ALA ARG VAL ALA MET GLY THR ALA CYS ASP100 105 110

LEU VAL GLY PRO ARG LEU ALA SER ALA ALA ILE ILE LEU LEU THR THR115 120 125

PRO ALA VAL TYR TYR SER ALA VAL ILE ASP SER ALA SER SER TYR LEU130 135 140

LEU VAL ARG PHE PHE THR GLY PHE SER LEU ALA SER PHE VAL SER THR145 150 155 160

GLN PHE TRP MET SER SER MET PHE SER PRO PRO LYS VAL GLY LEU ALA165 170 175

ASN GLY VAL ALA GLY GLY TRP GLY ASN LEU GLY GLY GLY ALA VAL GLN180 185 190

LEU ILE MET PRO LEU VAL PHE GLU ALA ILE ARG LYS ALA GLY ALA THR195 200 205

PRO PHE THR ALA TRP ARG VAL ALA PHE PHE VAL PRO GLY LEU LEU GLN210 215 220

THR LEU SER ALA VAL ALA VAL LEU ALA PHE GLY GLN ASP MET PRO ASP225 230 235 240

GLY ASN TYR ARG LYS LEU HIS ARG SER GLY ASP MET HIS LYS ASP SER245 250 255

PHE GLY ASN VAL LEU ARG HIS ALA VAL THR ASN TYR ARG ALA TRP ILE260 265 270

LEU ALA LEU THR TYR GLY TYR CYS PHE GLY VAL GLU LEU ALA VAL ASP275 280 285

ASN ILE VAL ALA GLN TYR PHE TYR ASP ARG PHE GLY VAL LYS LEU SER290 295 300

THR ALA GLY PHE ILE ALA ALA SER PHE GLY MET ALA ASN ILE VAL SER305 310 315 320

ARG PRO GLY GLY GLY LEU LEU SER ASP TRP LEU SER SER ARG PHE GLY325 330 335

MET ARG GLY ARG LEU TRP GLY LEU TRP VAL VAL GLN THR ILE GLY GLY340 345 350

VAL LEU CYS VAL VAL LEU GLY ALA VAL ASP TYR SER PHE ALA ALA SER355 360 365

VAL ALA VAL MET ILE LEU PHE SER MET PHE VAL GLN ALA ALA CYS GLY370 375 380LEU THR PHE GLY ILE VAL PRO PHE VAL SER ARG ARG SER LEU GLY LEU385 390 395 400

ILE SER GLY MET THR GLY GLY GLY GLY ASN VAL GLY ALA VAL LEU THR405 410 415

GLN LEU ILE PHE PHE HIS GLY SER LYS TYR LYS THR GLU THR GLY ILE420 425 430

LYS TYR MET GLY PHE MET ILE ILE ALA CYS THR LEU PRO ILE THR LEU435 440 445

ILE TYR PHE PRO GLN TRP GLY GLY MET PHE LEU GLY PRO ARG PRO GLY450 455 460

ALA THR ALA GLU ASP TYR TYR ASN ARG GLU TRP THR ALA HIS GLU CYS465 470 475 480

ASP LYS GLY PHE ASN THR ALA SER VAL ARG PHE ALA GLU ASN SER VAL485 490 495

ARG GLU GLY GLY ARG SER GLY SER GLN SER LYS HIS THR THR VAL PRO500 505 510

VAL GLU SER SER PRO ALA ASP VAL515 520

<210> 93

<211> 49597

<212> DNA

<213> VETOR

<400> 93

GTCTTGCTCG ACTCTAGAGC TCGTTCCTCG AGGCCTCGAG GCCTCGAGGA ACGGTACCTG 60

CGGGGAAGCT TACAATAATG TGTGTTGTTA AGTCTTGTTG CCTGTCATCG TCTGACTGAC 120

TTTCGTCATA AATCCCGGCC TCCGTAACCC AGCTTTGGGC AAGCTCACGG ATTTGATCCG 180

GCGGAACGGG AATATCGAGA TGCCGGGCTG AACGCTGCAG TTCCAGCTTT CCCTTTCGGG 240

ACAGGTACTC CAGCTGATTG ATTATCTGCT GAAGGGTCTT GGTTCCACCT CCTGGCACAA 300

TGCGAATGAT TACTTGAGCG CGATCGGGCA TCCAATTTTC TCCCGTCAGG TGCGTGGTCA 360

AGTGCTACAA GGCACCTTTC AGTAACGAGC GACCGTCGAT CCGTCGCCGG GATACGGACA 420

AAATGGAGCG CAGTAGTCCA TCGAGGGCGG CGAAAGCCTC GCCAAAAGCA ATACGTTCAT 480

CTCGCACAGC CTCCAGATCC GATCGAGGGT CTTCGGCGTA GGCAGATAGA AGCATGGATA 540

CATTGCTTGA GAGTATTCCG ATGGACTGAA GTATGGCTTC CATCTTTTCT CGTGTGTCTG 600

CATCTATTTC GAGAAAGCCC CCGATGCGGC GCACCGCAAC GCGAATTGCC ATACTATCCG 660

AAAGTCCCAG CAGGCGCGCT TGATAGGAAA AGGTTTCATA CTCGGCCGAT CGCAGACGGG 720

CACTCACGAC CTTGAACCCT TCAACTTTCA GGGATCGATG CTGGTTGATG GTAGTCTCAC 780

TCGACGTGGC TCTGGTGTGT TTTGACATAG CTTCCTCCAA AGAAAGCGGA AGGTCTGGAT 84 0

ACTCCAGCAC GAAATGTGCC CGGGTAGACG GATGGAAGTC TAGCCCTGCT CAATATGAAA 900

TCAACAGTAC ATTTACAGTC AATACTGAAT ATACTTGCTA CATTTGCAAT TGTCTTATAA 960

CGAATGTGAA ATAAAAATAG TGTAACAACG CTTTTACTCA TCGATAATCA CAAAAACATT 1020

TATACGAACA AAAATACAAA TGCACTCCGG TTTCACAGGA TAGGCGGGAT CAGAATATGC 1080

AACTTTTGAC GTTTTGTTCT TTCAAAGGGG GTGCTGGCAA AACCACCGCA CTCATGGGCC 1140

TTTGCGCTGC TTTGGCAAAT GACGGTAAAC GAGTGGCCCT CTTTGATGCC GACGAAAACC 1200

GGCCTCTGAC GCGATGGAGA GAAAACGCCT TACAAAGCAG TACTGGGATC CTCGCTGTGA 1260

AGTCTATTCC GCCGACGAAA TGCCCCTTCT TGAAGCAGCC TATGAAAATG CCGAGCTCGA 1320

AGGATTTGAT TATGCGTTGG CCGATACGCG TGGCGGCTCG AGCGAGCTCA ACAACACAAT 1380

CATCGCTAGC TCAAACCTGC TTCTGATCCC CACCATGCTA ACGCCGCTCG ACATCGATGA 1440

GGCACTATCT ACCTACCGCT ACGTCATCGA GCTGCTGTTG AGTGAAAATT TGGCAATTCC 1500TACAGCTGTT TTGCGCCAAC GCGTCCCGGT CGGCCGATTG ACAACATCGC AACGCAGGAT 1560

GTCAGAGACG CTAGAGAGCC TTCCAGTTGT ACCGTCTCCC ATGCATGAAA GAGATGCATT 1620

TGCCGCGATG AAAGAACGCG GCATGTTGCA TCTTACATTA CTAAACACGG GAACTGATCC 1680

GACGATGCGC CTCATAGAGA GGAATCTTCG GATTGCGATG GAGGAAGTCG TGGTCATTTC 1740

GAAACTGATC AGCAAAATCT TGGAGGCTTG AAGATGGCAA TTCGCAAGCC CGCATTGTCG 1800

GTCGGCGAAG CACGGCGGCT TGCTGGTGCT CGACCCGAGA TCCACCATCC CAACCCGACA 1860

CTTGTTCCCC AGAAGCTGGA CCTCCAGCAC TTGCCTGAAA AAGCCGACGA GAAAGACCAG 1920

CAACGTGAGC CTCTCGTCGC CGATCACATT TACAGTCCCG ATCGACAACT TAAGCTAACT 1980

GTGGATGCCC TTAGTCCACC TCCGTCCCCG AAAAAGCTCC AGGTTTTTCT TTCAGCGCGA 204 0

CCGCCCGCGC CTCAAGTGTC GAAAACATAT GACAACCTCG TTCGGCAATA CAGTCCCTCG 2100

AAGTCGCTAC AAATGATTTT AAGGCGCGCG TTGGACGATT TCGAAAGCAT GCTGGCAGAT 2160

GGATCATTTC GCGTGGCCCC GAAAAGTTAT CCGATCCCTT CAACTACAGA AAAATCCGTT 2220

CTCGTTCAGA CCTCACGCAT GTTCCCGGTT GCGTTGCTCG AGGTCGCTCG AAGTCATTTT 2280

GATCCGTTGG GGTTGGAGAC CGCTCGAGCT TTCGGCCACA AGCTGGCTAC CGCCGCGCTC 234 0

GCGTCATTCT TTGCTGGAGA GAAGCCATCG AGCAATTGGT GAAGAGGGAC CTATCGGAAC 24 00

CCCTCACCAA ATATTGAGTG TAGGTTTGAG GCCGCTGGCC GCGTCCTCAG TCACCTTTTG 24 60

AGCCAGATAA TTAAGAGCCA AATGCAATTG GCTCAGGCTG CCATCGTCCC CCCGTGCGAA 2520

ACCTGCACGT CCGCGTCAAA GAAATAACCG GCACCTCTTG CTGTTTTTAT CAGTTGAGGG 2580

CTTGACGGAT CCGCCTCAAG TTTGCGGCGC AGCCGCAAAA TGAGAACATC TATACTCCTG 264 0

TCGTAAACCT CCTCGTCGCG TACTCGACTG GCAATGAGAft GTTGCTCGCG CGATAGAACG 2700

TCGCGGGGTT TCTCTAAAAA CGCGAGGAGA AGATTGAACT CACCTGCCGT AAGTTTCACC 27 60

TCACCGCCAG CTTCGGACAT CAAGCGACGT TGCCTGAGAT TAAGTGTCCA GTCAGTAAAA 2820

CAAAAAGACC GTCGGTCTTT GGAGCGGACA ACGTTGGGGC GCACGCGCAA GGCAACCCGA 2880

ATGCGTGCAA GAAACTCTCT CGTACTAAAC GGCTTAGCGA TAAAATCACT TGCTCCTAGC 2 94 0

TCGAGTGCAA CAACTTTATC CGTCTCCTCA AGGCGGTCGC CACTGATAAT TATGATTGGA 3000

ATATCAGACT TTGCCGCCAG ATTTCGAACG ATCTCAAGCC CATCTTCACG ACCTAAATTT 3060

AGATCAACAA CCACGACATC GACCGTCGCG GAAGAGAGTA CTCTAGTGAA CTGGGTGCTG 3120

TCGGCTACCG CGGTCACTTT GAAGGCGTGG ATCGTAAGGT ATTCGATAAT AAGATGCCGC 3180

ATAGCGACAT CGTCATCGAT AAGAAGAACG TGTTTCAACG GCTCACCTTT CAATCTAAAA 324 0

TCTGAACCCT TGTTCACAGC GCTTGAGAAA TTTTCACGTG AAGGATGTAC AATCATCTCC 3300

AGCTAAATGG GCAGTTCGTC AGAATTGCGG CTGACCGCGG ATGACGAAAA TGCGAACCAA 3360

GTATTTCAAT TTTATGACAA AAGTTCTCAA TCGTTGTTAC AAGTGAAACG CTTCGAGGTT 3420

ACAGCTACTA TTGATTAAGG AGATCGCCTA TGGTCTCGCC CCGGCGTCGT GCGTCCGCCG 34 80

CGAGCCAGAT CTCGCCTACT TCATAAACGT CCTCATAGGC ACGGAATGGA ATGATGACAT 354 0

CGATCGCCGT AGAGAGCATG TCAATCAGTG TGCGATCTTC CAAGCTAGCA CCTTGGGCGC 3600

TACTTTTGAC AAGGGAAAAC AGTTTCTTGA ATCCTTGGAT TGGATTCGCG CCGTGTATTG 3660

TTGAAATCGA TCCCGGATGT CCCGAGACGA CTTCACTCAG ATAAGCCCAT GCTGCATCGT 3720

CGCGCATCTC GCCAAGCAAT ATCCGGTCCG GCCGCATACG CAGACTTGCT TGGAGCAAGT 37 80

GCTCGGCGCT CACAGCACCC AGCCCAGCAC CGTTCTTGGA GTAGAGTAGT CTAACATGAT 384 0

TATCGTGTGG AATGACGAGT TCGAGCGTAT CTTCTATGGT GATTAGCCTT TCCTGGGGGG 3900

GGATGGCGCT GATCAAGGTC TTGCTCATTG TTGTCTTGCC GCTTCCGGTA GGGCCACATA 3960

GCAACATCGT CAGTCGGCTG ACGACGCATG CGTGCAGAAA CGCTTCCAAA TCCCCGTTGT 4020

CAAAATGCTG AAGGATAGCT TCATCATCCT GATTTTGGCG TTTCCTTCGT GTCTGCCACT 4 080

GGTTCCACCT CGAAGCATCA TAACGGGAGG AGACTTCTTT AAGACCAGAA ACACGCGAGC 4140

TTGGCCGTCG AATGGTCAAG CTGACGGTGC CCGAGGGAAC GGTCGGCGGC AGACAGATTT 4 200

GTAGTCGTTC ACCACCAGGA AGTTCAGTGG CGCAGAGGGG GTTACGTGGT CCGACATCCT 4260

GCTTTCTCAG CGCGCCCGCT AAAATAGCGA TATCTTCAAG ATCATCATAA GAGACGGGCA 4 320

AAGGCATCTT GGTAAAAATG CCGGCTTGGC GCACAAATGC CTCTCCAGGT CGATTGATCG 4 380

CAATTTCTTC AGTCTTCGGG TCATCGAGCC ATTCCAAAAT CGGCTTCAGA AGAAAGCGTA 4 4 40

GTTGCGGATC CACTTCCATT TACAATGTAT CCTATCTCTA AGCGGAAATT TGAATTCATT 4 500

AAGAGCGGCG GTTCCTCCCC CGCGTGGCGC CGCCAGTCAG GCGGAGCTGG TAAACACCAA 4 560

AGAAATCGAG GTCCCGTGCT ACGAAAATGG AAACGGTGTC ACCCTGATTC TTCTTCAGGG 4 620

TTGGCGGTAT GTTGATGGTT GCCTTAAGGG CTGTCTCAGT TGTCTGCTCA CCGTTATTTT 4 680

GAAAGCTGTT GAAGCTCATC CCGCCACCCG AGCTGCCGGC GTAGGTGCTA GCTGCCTGGA 474 0

AGGCGCCTTG AACAACACTC AAGAGCATAG CTCCGCTAAA ACGCTGCCAG AAGTGGCTGT 4 800

CGACCGAGCC CGGCAATCCT GAGCGACCGA GTTCGTCCGC GCTTGGCGAT GTTAACGAGA 4 8 60

TCATCGCATG GTCAGGTGTC TCGGCGCGAT CCCACAACAC AAAAACGCGC CCATCTCCCT 4 920

GTTGCAAGCC ACGCTGTATT TCGCCAACAA CGGTGGTGCC ACGATCAAGA AGCACGATAT 4 980

TGTTCGTTGT TCCACGAATA TCCTGAGGCA AGACACACTT TACATAGCCT GCCAAATTTG 504 0TGTCGATTGC GGTTTGCAAG ATGCACGGAA TTATTGTCCC TTGCGTTACC ATAAAATCGG 5100

GGTGCGGCAA GAGCGTGGCG CTGCTGGGCT GCAGCTCGGT GGGTTTCATA CGTATCGACA 5160

AATCGTTCTC GCCGGACACT TCGCCATTCG GCAAGGAGTT GTCGTCACGC TTGCCTTCTT 5220

GTCTTCGGCC CGTGTCGCCC TGAATGGCGC GTTTGCTGAC CCCTTGATCG CCGCTGCTAT 5280

ATGCAAAAAT CGGTGTTTCT TCCGGCCGTG GCTCATGCCG CTCCGGTTCG CCCCTCGGCG 534 0

GTAGAGGAGC AGCAGGCTGA ACAGCCTCTT GAACCGCTGG AGGATCCGGC GGCACCTCAA 5400

TCGGAGCTGG ATGAAATGGC TTGGTGTTTG TTGCGATCAA AGTTGACGGC GATGCGTTCT 54 60

CATTCACCTT CTTTTGGCGC CCACCTAGCC AAATGAGGCT TAATGATAAC GCGAGAACGA 5520

CACCTCCGAC GATCAATTTC TGAGACCCCG AAAGACGCCG GCGATGTTTG TCGGAGACCA 5580

GGGATCCAGA TGCATCAACC TCATGTGCCG CTTGCTGACT ATCGTTATTC ATCCCTTCGC 564 0

CCCCTTCAGG ACGCGTTTCA CATCGGGCCT CACCGTGCCC GTTTGCGGCC TTTGGCCAAC 5700

GGGATCGTAA GCGGTGTTCC AGATACATAG TACTGTGTGG CCATCCCTCA GACGCCAACC 57 60

TCGGGAAACC GAAGAAATCT CGACATCGCT CCCTTTAACT GAATAGTTGG CAACAGCTTC 5820

CTTGCCATCA GGATTGATGG TGTAGATGGA GGGTATGCGT ACATTGCCCG GAAAGTGGAA 5880

TACCGTCGTA AATCCATTGT CGAAGACTTC GAGTGGCAAC AGCGAACGAT CGCCTTGGGC 594 0

GACGTAGTGC CAATTACTGT CCGCCGCACC AAGGGCTGTG ACAGGCTGAT CCAATAAATT 6000

CTCAGCTTTC CGTTGATATT GTGCTTCCGC GTGTAGTCTG TCCACAACAG CCTTCTGTTG 6060

TGCCTCCCTT CGCCGAGCCG CCGCATCGTC GGCGGGGTAG GCGAATTGGA CGCTGTAATA 6120

GAGATCGGGC TGCTCTTTAT CGAGGTGGGA CAGAGTCTTG GAACTTATAC TGAAAACATA 6180

ACGGCGCATC CCGGAGTCGC TTGCGGTTAG CACGATTACT GGCTGAGGCG TGAGGACCTG 624 0

GCTTGCCTTG AAAAATAGAT AATTTCCCCG CGGTAGGGCT GCTAGATCTT TGCTATTTGA 6300

AACGGCAACC GCTGTCACCG TTTCGTTCGT GGCGAATGTT ACGACCAAAG TAGCTCCAAC 6360

CGCCGTCGAG AGGCGCACCA CTTGATCGGG ATTGTAAGCC AAATAACGCA TGCGCGGATC 6420

TAGCTTGCCC GCCATTGGAG TGTCTTCAGC CTCCGCACCA GTCGCAGCGG CAAATAAACA 6480

TGCTAAAATG AAAAGTGCTT TTCTGATCAT GGTTCGCTGT GGCCTACGTT TGAAACGGTA 654 0

TCTTCCGATG TCTGATAGGA GGTGACAACC AGACCTGCCG GGTTGGTTAG TCTCAATCTG 6600

CCGGGCAAGC TGGTCACCTT TTCGTAGCGA ACTGTCGCGG TCCACGTACT CACCACAGGC 6660

ATTTTGCCGT CAACGACGAG GGTCCTTTTA TAGCGAATTT GCTGCGTGCT TGGAGTTACA 6720

TCATTTGAAG CGATGTGCTC GACCTCCACC CTGCCGCGTT TGCCAAGAAT GACTTGAGGC 6780

GAACTGGGAT TGGGATAGTT GAAGAATTGC TGGTAATCCT GGCGCACTGT TGGGGCACTG 6840

AAGTTCGATA CCAGGTCGTA GGCGTACTGA GCGGTGTCGG CATCATAACT CTCGCGCAGG 6900

CGAACGTACT CCCACAATGA GGCGTTAACG ACGGCCTCCT CTTGAGTTGC AGGCAATCGC 6960

GAGACAGACA CCTCGCTGTC AACGGTGCCG TCCGGCCGTA TCCATAGATA TACGGGCACA 7020

AGCCTGCTCA ACGGCACCAT TGTGGCTATA GCGAACGCTT GAGCAACATT TCCCAAAATC 7 080

GCGATAGCTG CGACAGCTGC AATGAGTTTG GAGAGACGTC GCGCCGATTT CGCTCGCGCG 714 0

GTTTGAAAGG CTTCTACTTC CTTATAGTGC TCGGCAAGGC TTTCGCGCGC CACTAGCATG 7200

GCATATTCAG GCCCCGTCAT AGCGTCCACC CGAATTGCCG AGCTGAAGAT CTGACGGAGT 7 260

AGGCTGCCAT CGCCCCACAT TCAGCGGGAA GATCGGGCCT TTGCAGCTCG CTAATGTGTC 7 320

GTTTGTCTGG CAGCCGCTCA AAGCGACAAC TAGGCACAGC AGGCAATACT TCATAGAATT 7 380

CTCCATTGAG GCGAATTTTT GCGCGACCTA GCCTCGCTCA ACCTGAGCGA AGCGACGGTA 74 40

CAAGCTGCTG GCAGATTGGG TTGCGCCGCT CCAGTAACTG CCTCCAATGT TGCCGGCGAT 7 500

CGCCGGCAAA GCGACAATGA GCGCATCCCC TGTCAGAAAA AACATATCGA GTTCGTAAAG 7560

ACCTVATGATC TTGGCCGCGG TCGTACCGGC GAAGGTGATT ACACCAAGCA TAAGGGTGAG 7 620

CGCAGTCGCT TCGGTTAGGA TGACGATCGT TGCCACGAGG TTTAAGAGGA GAAGCAAGAG 7 680

ACCGTAGGTG ATAAGTTGCC CGATCCACTT AGCTGCGATG TCCCGCGTGC GATCAAAAAT 7740

ATATCCGACG AGGATCAGAG GCCCGATCGC GAGAAGCACT TTCGTGAGAA TTCCAACGGC 7800

GTCGTAAACT CCGAAGGCAG ACCAGAGCGT GCCGTAAAGG ACCCACTGTG CCCCTTGGAA 7860

AGCAAGGATG TCCTGGTCGT TCATCGGACC GATTTCGGAT GCGATTTTCT GAAAAACGGC 7 920

CTGGGTCACG GCGAACATTG TATCCAACTG TGCCGGAACA GTCTGCAGAG GCAAGCCGGT 7 980

TACACTAAAC TGCTGAACAA AGTTTGGGAC CGTCTTTTCG AAGATGGAAA CCACATAGTC 8040

TTGGTAGTTA GCCTGCCCAA CAATTAGAGC AACAACGATG GTGACCGTGA TCACCCGAGT 8100

GATACCGCTA CGGGTATCGA CTTCGCCGCG TATGACTAAA ATACCCTGAA CAATAATCCA 8160

AAGAGTGACA CAGGCGATCA ATGGCGCACT CACCGCCTCC TGGATAGTCT CAAGCATCGA 8220

GTCCAAGCCT GTCGTGAAGG CTACATCGAA GATCGTATGA ATGGCCGTAA ACGGCGCCGG 8280

AATCGTGAAA TTCATCGATT GGACCTGAAC TTGACTGGTT TGTCGCATAA TGTTGGATAA 834 0

AATGAGCTCG CATTCGGCGA GGATGCGGGC GGATGAACAA ATCGCCCAGC CTTAGGGGAG 8400

GGCACCAAAG ATGACAGCGG TCTTTTGATG CTCCTTGCGT TGAGCGGCCG CCTCTTCCGC 84 60

CTCGTGAAGG CCGGCCTGCG CGGTAGTCAT CGTTAATAGG CTTGTCGCCT GTACATTTTG 8520

AATCATTGCG TCATGGATCT GCTTGAGAAG CAAACCATTG GTCACGGTTG CCTGCATGAT 8580ATTGCGAGAT CGGGAAAGCT GAGCAGACGT ATCAGCATTC GCCGTCAAGC GTTTGTCCAT 8 64 0

CGTTTCCAGA TTGTCAGCCG CAATGCCAGC GCTGTTTGCG GAACCGGTGA TCTGCGATCG 8700

CAACAGGTCC GCTTCAGCAT CACTACCCAC GACTGCACGA TCTGTATCGC TGGTGATCGC 87 60

ACGTGCCGTG GTCGACATTG GCATTCGCGG CGAAAACATT TCATTGTCTA GGTCCTTCGT 8820

CGAAGGATAC TGATTTTTCT GGTTGAGCGA AGTCAGTAGT CCAGTAACGC CGTAGGCCGA 8880

CGTCAACATC GTAACCATCG CTATAGTCTG AGTGAGATTC TCCGCAGTCG CGAGCGCAGT 8940

CGCGAGCGTC TCAGCCTCCG TTGCCGGGTC GCTAACAACA AACTGCGCCC GCGCGGGCTG 9000

AATATATAGA AAGCTGCAGG TCAAAACTGT TGCAATAAGT TGCGTCGTCT TCATCGTTTC 9060

CTACCTTATC AATCTTCTGC CTCGTGGTGA CGGGCCATGA ATTCGCTGAG CCAGCCAGAT 9120

GAGTTGCCTT CTTGTGCCTC GCGTAGTCGA GTTGCAAAGC GCACCGTGTT GGCACGCCCC 9180

GAAAGCACGG CGACATATTC ACGCATATCC CGCAGATCAA ATTCGCAGAT GACGCTTCCA 924 0

CTTTCTCGTT TAAGAAGAAA CTTACGGCTG CCGACCGTCA TGTCTTCACG GATCGCCTGA 9300

AATTCCTTTT CGGTACATTT CAGTCCATCG ACATAAGCCG ATCGATCTGC GGTTGGTGAT 9360

GGATAGAAAA TCTTCGTCAT ACATTGCGCA ACCAAGCTGG CTCCTAGCGG CGATTCCAGA 94 20

ACATGCTCTG GTTGCTGCGT TGCCAGTATT AGCATCCCGT TGTTTTTTCG AACGGTCAGG 94 80

AGGAATTTGT CGACGACAGT CGAAAATTTA GGGTTTAACA AATAGGCGCG AAACTCATCG 9540

CAGCTCATCA CAAAACGGCG GCCGTCGATC ATGGCTCCAA TCCGATGCAG GAGATATGCT 9600

GCAGCGGGAG CGCATACTTC CTCGTATTCG AGAAGATGCG TCATGTCGAA GCCGGTAATC 9660

GACGGATCTA ACTTTACTTC GTCAACTTCG CCGTCAAATG CCCAGCCAAG CGCATGGCCC 9720

CGGCACCAGC GTTGGAGCCG CGCTCCTGCG CCTTCGGCGG GCCCATGCAA CAAAAATTCA 97 80

CGTAACCCCG CGATTGAACG CATTTGTGGA TCAAACGAGA GCTGACGATG GATACCACGG 984 0

ACCAGACGGC GGTTCTCTTC CGGAGAAATC CCACCCCGAC CATCACTCTC GATGAGAGCC 9900

ACGATCCATT CGCGCAGAAA ATCGTGTGAG GCTGCTGTGT TTTCTAGGCC ACGCAACGGC 9960

GCCAACCCGC TGGGTGTGCC TCTGTGAAGT GCCAAATATG TTCCTCCTGT GGCGCGAACC 10020

AGCAATTCGC CACCCCGGTC CTTGTCAAAG AACACGACCG TACCTGCACG GTCGACCATG 10080

CTCTGTTCGA GCATGGCTAG AACAAACATC ATGAGCGTCG TCTTACCCCT CCCGATAGGC 10140

CCGAATATTG CCGTCATGCC AACATCGTGC TCATGCGGGA TATAGTCGAA AGGCGTTCCG 10200

CCATTGGTAC GAAATCGGGC AATCGCGTTG CCCCAGTGGC CTGAGCTGGC GCCCTCTGGA 10260

AAGTTTTCGA AAGAGACAAA CCCTGCGAAA TTGCGTGAAG TGATTGCGCC AGGGCGTGTG 10320

CGCCACTTAA AATTCCCCGG CAATTGGGAC CAATAGGCCG CTTCCATACC AATACCTTCT 10380

TGGACAACCA CGGCACCTGC ATCCGCCATT CGTGTCCGAG CCCGCGCGCC CCTGTCCCCA 10440

AGACTATTGA GATCGTCTGC ATAGACGCAA AGGCTCAAAT GATGTGAGCC CATAACGAAT 10500

TCGTTGCTCG CAAGTGCGTC CTCAGCCTCG GATAATTTGC CGATTTGAGT CACGGCTTTA 10560

TCGCCGGAAC TCAGCATCTG GCTCGATTTG AGGCTAAGTT TCGCGTGCGC TTGCGGGCGA 10620

GTCAGGAACG AAAAACTCTG CGTGAGAACA AGTGGAAAAT CGAGGGATAG CAGCGCGTTG 10680

AGCATGCCCG GCCGTGTTTT TGCAGGGTAT TCGCGAAACG AATAGATGGA TCCAACGTAA 10740

CTGTCTTTTG GCGTTCTGAT CTCGAGTCCT CGCTTGCCGC AAATGACTCT GTCGGTATAA 10800

ATCGAAGCGC CGAGTGAGCC GCTGACGACC GGAACCGGTG TGAACCGACC AGTCATGATC 108 60

AACCGTAGCG CTTCGCCAAT TTCGGTGAAG AGCACACCCT GCTTCTCGCG GATGCCAAGA 10920

CGATGCAGGC CATACGCTTT AAGAGAGCCA GCGACAACAT GCCAAAGATC TTCCATGTTC 10980

CTGATCTGGC CCGTGAGATC GTTTTCCCTT TTTCCGCTTA GCTTGGTGAA CCTCCTCTTT 1104 0

ACCTTCCCTA AAGCCGCCTG TGGGTAGACA ATCAACGTAA GGAAGTGTTC ATTGCGGAGG 11100

AGTTGGCCGG AGAGCACGCG CTGTTCAAAA GCTTCGTTCA GGCTAGCGGC GAAAACACTA 11160

CGGAAGTGTC GCGGCGCCGA TGATGGCACG TCGGCATGAC GTACGAGGTG AGCATATATT 11220

GACACATGAT CATCAGCGAT ATTGCGCAAC AGCGTGTTGA ACGCACGACA ACGCGCATTG 11280

CGCATTTCAG TTTCCTCAAG CTCGAATGCA ACGCCATCAA TTCTCGCAAT GGTCATGATC 11340

GATCCGTCTT CAAGAAGGAC GATATGGTCG CTGAGGTGGC CAATATAAGG GAGATAGATC 114 00

TCACCGGATC TTTCGGTCGT TCCACTCGCG CCGAGCATCA CACCATTCCT CTCCCTCGTG 114 60

GGGGAACCCT AATTGGATTT GGGCTAACAG TAGCGCCCCC CCAAACTGCA CTATCAATGC 11520

TTCTTCCCGC GGTCCGCAAA AATAGCAGGA CGACGCTCGC CGCATTGTAG TCTCGCTCCA 11580

CGATGAGCCG GGCTGCAAAC CATAACGGCA CGAGAACGAC TTCGTAGAGC GGGTTCTGAA 11640

CGATAACGAT GACAAAGCCG GCGAACATCA TGAATAACCC TGCCAATGTC AGTGGCACCC 11700

CAAGAAACAA TGCGGGCCGT GTGGCTGCGA GGTAAAGGGT CGATTCTTCC AAACGATCAG 117 60

CCATCAACTA CCGCCAGTGA GCGTTTGGCC GAGGAAGCTC GCCCCAAACA TGATAACAAT 11820

GCCGCCGACG ACGCCGGCAA CCAGCCCAAG CGAAGCCCGC CCGAACATCC AGGAGATCCC 11880

GATAGCGACA ATGCCGAGAA CAGCGAGTGA CTGGCCGAAC GGACCAAGGA TAAACGTGCA 11940

TATATTGTTA ACCATTGTGG CGGGGTCAGT GCCGCCACCC GCAGATTGCG CTGCGGCGGG 12000

TCCGGATGAG GAAATGCTCC ATGCAATTGC ACCGCACAAG CTTGGGGCGC AGCTCGATAT 12060

CACGCGCATC ATCGCATTCG AGAGCGAGAG GCGATTTAGA TGTAAACGGT ATCTCTCAAA 12120GCATCGCATC AATGCGCACC TCCTTAGTAT AAGTCGAATA AGACTTGATT GTCGTCTGCG 12180

GATTTGCCGT TGTCCTGGTG TGGCGGTGGC GGAGCGATTA AACCGCCAGC GCCATCCTCC 12240

TGCGAGCGGC GCTGATATGA CCCCCAAACA TCCCACGTCT CTTCGGATTT TAGCGCCTCG 12300

TGATCGTCTT TTGGAGGCTC GATTAACGCG GGCACCAGCG ATTGAGCAGC TGTTTCAACT 12360

TTTCGCACGT AGCCGTTTGC AAAACCGCCG ATGAAATTAC CGGTGTTGTA AGCGGAGATC 12420

GCCCGACGAA GCGCAAATTG CTTCTCGTCA ATCGTTTCGC CGCCTGCATA ACGACTTTTC 124 80

AGCATGTTTG CAGCGGCAGA TAATGATGTG CACGCCTGGA GCGCACCGTC AGGTGTCAGA 1254 0

CCGAGCATAG AAAAATTTCG AGAGTTTATT TGCATGAGGC CAACATCCAG CGAATGCCGT 12600

GCATCGAGAC GGTGCCTGAC GACTTGGGTT GCTTGGCTGT GATCTTGCCA GTGAAGCGTT 12660

TCGCCGGTCG TGTTGTCATG AATCGCTAAA GGATCAAAGC GACTCTCCAC CTTAGCTATC 12720

GCCGCAAGCG TAGATGTCGC AACTGATGGG GCACACTTGC GAGCAACATG GTCAAACTCA 12780

GCAGATGAGA GTGGCGTGGC AAGGCTCGAC GAACAGAAGG AGACCATCAA GGCAAGAGAA 12840

AGCGACCCCG ATCTCTTAAG CATACCTTAT CTCCTTAGCT CGCAACTAAC ACCGCCTCTC 12900

CCGTTGGAAG AAGTGCGTTG TTTTATGTTG AAGATTATCG GGAGGGTCGG TTACTCGAAA 12960

ATTTTCAATT GCTTCTTTAT GATTTCAATT GAAGCGAGAA ACCTCGCCCG GCGTCTTGGA 13020

ACGCAACATG GACCGAGAAC CGCGCATCCA TGACTAAGCA ACCGGATCGA CCTATTCAGG 13080

CCGCAGTTGG TCAGGTCAGG CTCAGAACGA AAATGCTCGG CGAGGTTACG CTGTCTGTAA 1314 0

ACCCATTCGA TGAACGGGAA GCTTCCTTCC GATTGCTCTT GGCAGGAATA TTGGCCCATG 13200

CCTGCTTGCG CTTTGCAAAT GCTCTTATCG CGTTGGTATC ATATGCCTTG TCCGCCAGCA 132 60

GAAACGCACT CTAAGCGATT ATTTGTAAAA ATGTTTCGGT CATGCGGCGG TCATGGGCTT 13320

GACCCGCTGT CAGCGCAAGA CGGATCGGTC AACCGTCGGC ATCGACAACA GCGTGAATCT 13380

TGGTGGTCAA ACCGCCACGG GAACGTCCCA TACAGCCATC GTCTTGATCC CGCTGTTTCC 13440

CGTCGCCGCA TGTTGGTGGA CGCGGACACA GGAACTGTCA ATCATGACGA CATTCTATCG 13500

AAAGCCTTGG AAATCACACT CAGAATATGA TCCCAGACGT CTGCCTCACG CCATCGTACA 13560

AAGCGATTGT AGCAGGTTGT ACAGGAACCG TATCGATCAG GAACGTCTGC CCAGGGCGGG 13620

CCCGTCCGGA AGCGCCACAA GATGACATTG ATCACCCGCG TCAACGCGCG GCACGCGACG 13680

CGGCTTATTT GGGAACAAAG GACTGAACAA CAGTCCATTC GAAATCGGTG ACATCAAAGC 1374 0

GGGGACGGGT TATCAGTGGC CTCCAAGTCA AGCCTCAATG AATCAAAATC AGACCGATTT 13800

GCAAACCTGA TTTATGAGTG TGCGGCCTAA ATGATGAAAT CGTCCTTCTA GATCGCCTCC 138 60

GTGGTGTAGC AACACCTCGC AGTATCGCCG TGCTGACCTT GGCCAGGGAA TTGACTGGCA 13920

AGGGTGCTTT CACATGACCG CTCTTTTGGC CGCGATAGAT GATTTCGTTG CTGCTTTGGG 13980

CACGTAGAAG GAGAGAAGTC ATATCGGAGA AATTCCTCCT GGCGCGAGAG CCTGCTCTAT 14 040

CGCGACGGCA TCCCACTGTC GGGAACAGAC CGGATCATTC ACGAGGCGAA AGTCGTCAAC 14100

ACATGCGTTA TAGGCATCTT CCCTTGAAGG ATGATCTTGT TGCTGCCAAT CTGGAGGTGC 14160

GGCAGCCGCA GGCAGATGCG ATCTCAGCGC AACTTGCGGC AAAACATCTC ACTCACCTGA 14 220

AAACCACTAG CGAGTCTCGC GATCAGACGA AGGCCTTTTA CTTAACGACA CAATATCCGA 14 280

TGTCTGCATC ACAGGCGTCG CTATCCCAGT CAATACTAAA GCGGTGCAGG AACTAAAGAT 14 340

TACTGATGAC TTAGGCGTGC CACGAGGCCT GAGACGACGC GCGTAGACAG TTTTTTGAAA 14 4 00

TCATTATCAA AGTGATGGCC TCCGCTGAAG CCTATCACCT CTGCGCCGGT CTGTCGGAGA 14 4 60

GATGGGCAAG CATTATTACG GTCTTCGCGC CCGTACATGC ATTGGACGAT TGCAGGGTCA 14 520

ATGGATCTGA GATCATCCAG AGGATTGCCG CCCTTACCTT CCGTTTCGAG TTGGAGCCAG 14580

CCCCTAAATG AGACGACATA GTCGACTTGA TGTGACAATG CCAAGAGAGA GATTTGCTTA 14 640

ACCCGATTTT TTTGCTCAAG CGTAAGCCTA TTGAAGCTTG CCGGCATGAC GTCCGCGCCG 14700

AAAGAATATC CTACAAGTAA AACATTCTGC ACACCGAAAT GCTTGGTGTA GACATCGATT 14 7 60

ATGTGACCAA GATCCTTAGC AGTTTCGCTT GGGGACCGCT CCGACCAGAA ATACCGAAGT 14820

GAACTGACGC CAATGACAGG AATCCCTTCC GTCTGCAGAT AGGTACCATC GATAGATCTG 14 880

CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 14 940

GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 15000

GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA 15060

TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 15120

GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG 15180

CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 15240

GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 15300

GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 15360

CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 15420

GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 154 80

ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 15540

CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 15600

GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 15 660TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 15720

AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 15780

ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 15840

GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 15900

AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 15960

GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 16020

AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 16080

ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 1614 0

GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 16200

ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 16260

CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 16320

CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 16380

AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGGGG GGGGGGGGGG 16440

GGGTTCCATT GTTCATTCCA CGGACAAAAA CAGAGAAAGG AAACGACAGA GGCCAAAAAG 16500

CTCGCTTTCA GCACCTGTCG TTTCCTTTCT TTTCAGAGGG TATTTTAAAT AAAAACATTA 16560

AGTTATGACG AAGAAGAACG GAAACGCCTT AAACCGGAAA ATTTTCATAA ATAGCGAAAA 16620

CCCGCGAGGT CCCTGTCGGA TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT 16680

ACATATCACA ACGTGCGTGG AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG 16740

TATCGTATTA ATTGATCTGC ATCAACTTAA CGTAAAAACA ACTTCAGACA ATACAAATCA 16800

GCGACACTGA ATACGGGGCA ACCTCATGTC CCCCCCCCCC CCCCCCCTGC AGGCATCGTG 168 60

GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 16920

GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 16980

GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 17040

CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 17100

TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT 17160

ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 17220

AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 17280

AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 17340

CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 17 4 00

CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 17 4 60

GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 17 520

CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 17 580

AGGCCCTTTC GTCTTCAAGA ATTGGTCGAC GATCTTGCTG CGTTCGGATA TTTTCGTGGA 17 64 0

GTTCCCGCCA CAGACCCGGA TTGAAGGCGA GATCCAGCAA CTCGCGCCAG ATCATCCTGT 17 700

GACGGAACTT TGGCGCGTGA TGACTGGCCA GGACGTCGGC CGAAAGAGCG ACAAGCAGAT 17760

CACGCTTTTC GACAGCGTCG GATTTGCGAT CGAGGATTTT TCGGCGCTGC GCTACGTCCG 17 820

CGACCGCGTT GAGGGATCAA GCCACAGCAG CCCACTCGAC CTTCTAGCCG ACCCAGACGA 17 880

GCCAAGGGAT CTTTTTGGAA TGCTGCTCCG TCGTCAGGCT TTCCGACGTT TGGGTGGTTG 17940

AACAGAAGTC ATTATCGTAC GGAATGCCAA GCACTCCCGA GGGGAACCCT GTGGTTGGCA 18000

TGCACATACA AATGGACGAA CGGATAAACC TTTTCACGCC CTTTTAAATA TCCGTTATTC 18060

TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT 18120

TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT 18180

ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA 1824 0

CGTTGAAGGA GCCACTCAGC AAGCTGGTAC GATTGTAATA CGACTCACTA TAGGGCGAAT 18300

TGAGCGCTGT TTAAACGCTC TTCAACTGGA AGAGCGGTTA CCCGGACCGA AGCTTGCATG 18360

CCTGCAGTGC AGCGTGACCC GGTCGTGCCC CTCTCTAGAG ATAATGAGCA TTGCATGTCT 18420

AAGTTATAAA AAATTACCAC ATATTTTTTT TGTCACACTT GTTTGAAGTG CAGTTTATCT 184 80

ATCTTTATAC ATATATTTAA ACTTTACTCT ACGAATAATA TAATCTATAG TACTACAATA 1854 0

ATATCAGTGT TTTAGAGAAT CATATAAATG AACAGTTAGA CATGGTCTAA AGGACAATTG 18600

AGTATTTTGA CAACAGGACT CTACAGTTTT ATCTTTTTAG TGTGCATGTG TTCTCCTTTT 18 660

TTTTTGCAAA TAGCTTCACC TATATAATAC TTCATCCATT TTATTAGTAC ATCCATTTAG 18720

GGTTTAGGGT TAATGGTTTT TATAGACTAA TTTTTTTAGT ACATCTATTT TATTCTATTT 18780

TAGCCTCTAA ATTAAGAAAA CTAAAACTCT ATTTTAGTTT TTTTATTTAA TAATTTAGAT 1884 0

ATAAAATAGA ATAAAATAAA GTGACTAAAA ATTAAACAAA TACCCTTTAA GAAATTAAAA 18 900

AAACTAAGGA AACATTTTTC TTGTTTCGAG TAGATAATGC CAGCCTGTTA AACGCCGTCG 18 960

ACGAGTCTAA CGGACACCAA CCAGCGAACC AGCAGCGTCG CGTCGGGCCA AGCGAAGCAG 19020

ACGGCACGGC ATCTCTGTCG CTGCCTCTGG ACCCCTCTCG AGAGTTCCGC TCCACCGTTG 19080

GACTTGCTCC GCTGTCGGCA TCCAGAAATT GCGTGGCGGA GCGGCAGACG TGAGCCGGCA 1914 0

CGGCAGGCGG CCTCCTCCTC CTCTCACGGC ACGGCAGCTA CGGGGGATTC CTTTCCCACC 19200GCTCCTTCGC TTTCCCTTCC TCGCCCGCCG TAATAAATAG ACACCCCCTC CACACCCTCT 19260

TTCCCCAACC TCGTGTTGTT CGGAGCGCAC ACACACACAA CCAGATCTCC CCCAAATCCA 19320

CCCGTCGGCA CCTCCGCTTC AAGGTACGCC GCTCGTCCTC CCCCCCCCCC CCTCTCTACC 19380

TTCTCTAGAT CGGCGTTCCG GTCCATGGTT AGGGCCCGGT AGTTCTACTT CTGTTCATGT 19440

TTGTGTTAGA TCCGTGTTTG TGTTAGATCC GTGCTGCTAG CGTTCGTACA CGGATGCGAC 19500

CTGTACGTCA GACACGTTCT GATTGCTAAC TTGCCAGTGT TTCTCTTTGG GGAATCCTGG 19560

GATGGCTCTA GCCGTTCCGC AGACGGGATC GATTTCATGA TTTTTTTTGT TTCGTTGCAT 19620

AGGGTTTGGT TTGCCCTTTT CCTTTATTTC AATATATGCC GTGCACTTGT TTGTCGGGTC 19680

ATCTTTTCAT GCTTTTTTTT GTCTTGGTTG TGATGATGTG GTCTGGTTGG GCGGTCGTTC 19740

TAGATCGGAG TAGAATTCTG TTTCAAACTA CCTGGTGGAT TTATTAATTT TGGATCTGTA 19800

TGTGTGTGCC ATACATATTC ATAGTTACGA ATTGAAGATG ATGGATGGAA ATATCGATCT 198 60

AGGATAGGTA TACATGTTGA TGCGGGTTTT ACTGATGCAT ATACAGAGAT GCTTTTTGTT 19920

CGCTTGGTTG TGATGATGTG GTGTGGTTGG GCGGTCGTTC ATTCGTTCTA GATCGGAGTA 19980

GAATACTGTT TCAAACTACC TGGTGTATTT ATTAATTTTG GAACTGTATG TGTGTGTCAT 2004 0

ACATCTTCAT AGTTACGAGT TTAAGATGGA TGGAAATATC GATCTAGGAT AGGTATACAT 20100

GTTGATGTGG GTTTTACTGA TGCATATACA TGATGGCATA TGCAGCATCT ATTCATATGC 20160

TCTAACCTTG AGTACCTATC TATTATAATA AACAAGTATG TTTTATAATT ATTTTGATCT 20220

TGATATACTT GGATGATGGC ATATGCAGCA GCTATATGTG GATTTTTTTA GCCCTGCCTT 20280

CATACGCTAT TTATTTGCTT GGTACTGTTT CTTTTGTCGA TGCTCACCCT GTTGTTTGGT 2034 0

GTTACTTCTG CAGGTCGACT CTAGAGGATC TACAAGTTTG TACAAAAAAG CAGGCTCCGC 204 00

GGCCGCCCCC TTCACCATGG CTCGGCAGCA AAGCGTGCAG GCCTTGTGTG TGCTGGCGGC 204 60

GCTTCTCTTC GCCGCCTCCC TGCCGTCGCC GGCCGCCGCG GGGGTGCACC TCTCCTCGCT 20520

GCCCAAAGCG CTCGACGTCA CCACCTCCGC CAAACCCGGC CAAGTCCTGC ACGCCGGCGT 20580

GGACTCGCTG ACGGTGACGT GGAGCCTGAA CGCCACGGAG CCGGCCGGCG CCGACGCCGG 2064 0

GTACAAGGGC GTGAAGGTGA AGCTGTGCTA CGCGCCGGCG AGCCAGAAGG ACCGCGGGTG 20700

GCGCAAGTCC GAGGACGACA TCAGCAAGGA CAAGGCGTGC CAGTTCAAGG TCACCGAGCA 207 60

GGCGTACGCG GCGGCGGCGC CCGGCAGCTT CCAGTACGCC GTCGCCCGCG ACGTCCCCTC 20820

GGGCTCCTAC TACCTGCGCG CCTTCGCCAC GGACGCGTCG GGCGCCGAGG TGGCCTACGG 20880

CCAGACGGCG CCCACCGCCG CCTTCGACGT CGCCGGCATC ACCGGCATCC ACGCCTCTCT 20940

CAAGATCGCC GCCGGCGTCT TCTCGGCCTT CTCCGTCGTC GCGCTCGCCT TCTTCTTCGT 21000

CATCGAGACC CGCAAGAAGA ACAAGTAGAA GGGTGGGCGC GCCGACCCAG CTTTCTTGTA 21060

CAAAGTGGTG TTAACCTAGA CTTGTCCATC TTCTGGATTG GCCAACTTAA TTAATGTATG 21120

AAATAAAAGG ATGCACACAT AGTGACATGC TAATCACTAT AATGTGGGCA TCAAAGTTGT 21180

GTGTTATGTG TAATTACTAG TTATCTGAAT AAAAGAGAAA GAGATCATCC ATATTTCTTA 21240

TCCTAAATGA ATGTCACGTG TCTTTATAAT TCTTTGATGA ACCAGATGCA TTTCATTAAC 21300

CAAATCCATA TACATATAAA TATTAATCAT ATATAATTAA TATCAATTGG GTTAGCAAAA 21360

CAAATCTAGT CTAGGTGTGT TTTGCGAATT GCGGCCGCCA CCGCGGTGGA GCTCGAATTC 21420

CGGTCCGGGT CACCTTTGTC CACCAAGATG GAACTGCGGC CGCTCATTAA TTAAGTCAGG 214 80

CGCGCCTCTA GTTGAAGACA CGTTCATGTC TTCATCGTAA GAAGACACTC AGTAGTCTTC 2154 0

GGCCAGAATG GCCATCTGGA TTCAGCAGGC CTAGAAGGCC ATTTAAATCC TGAGGATCTG 21600

GTCTTCCTAA GGACCCGGGC GGTCCGATTA AACTTTAATT CGGACCGAAG CTTGCATGCC 21660

TGCAGTGCAG CGTGACCCGG TCGTGCCCCT CTCTAGAGAT AATGAGCATT GCATGTCTAA 21720

GTTATAAAAA ATTACCACAT ATTTTTTTTG TCACACTTGT TTGAAGTGCA GTTTATCTAT 21780

CTTTATACAT ATATTTAAAC TTTACTCTAC GAATAATATA ATCTATAGTA CTACAATAAT 21840

ATCAGTGTTT TAGAGAATCA TATAAATGAA CAGTTAGACA TGGTCTAAAG GACAATTGAG 21900

TATTTTGACA ACAGGACTCT ACAGTTTTAT CTTTTTAGTG TGCATGTGTT CTCCTTTTTT 21960

TTTGCAAATA GCTTCACCTA TATAATACTT CATCCATTTT ATTAGTACAT CCATTTAGGG 22020

TTTAGGGTTA ATGGTTTTTA TAGACTAATT TTTTTAGTAC ATCTATTTTA TTCTATTTTA 22080

GCCTCTAAAT TAAGAAAACT AAAACTCTAT TTTAGTTTTT TTATTTAATA ATTTAGATAT 22140AAAATAGAAT AAAATAAAGT GACTAAAAAT TAAACAAATA CCCTTTAAGA AATTAAAAAA 22200

ACTAAGGAAA CATTTTTCTT GTTTCGAGTA GATAATGCCA GCCTGTTAAA CGCCGTCGAC 222 60

GAGTCTAACG GACACCAACC AGCGAACCAG CAGCGTCGCG TCGGGCCAAG CGAAGCAGAC 22320GGCACGGCAT CTCTGTCGCT GCCTCTGGAC CCCTCTCGAG AGTTCCGCTC CACCGTTGGA 22380

CTTGCTCCGC TGTCGGCATC CAGAAATTGC GTGGCGGAGC GGCAGACGTG AGCCGGCACG 224 4 0

GCAGGCGGCC TCCTCCTCCT CTCACGGCAC CGGCAGCTAC GGGGGATTCC TTTCCCACCG 22500

CTCCTTCGCT TTCCCTTCCT CGCCCGCCGT AATAAATAGA CACCCCCTCC ACACCCTCTT 22560

TCCCCAACCT CGTGTTGTTC GGAGCGCACA CACACACAAC CAGATCTCCC CCAAATCCAC 22620

CCGTCGGCAC CTCCGCTTCA AGGTACGCCG CTCGTCCTCC CCCCCCCCCC TCTCTACCTT 22680

CTCTAGATCG GCGTTCCGGT CCATGCATGG TTAGGGCCCG GTAGTTCTAC TTCTGTTCAT 227 4 0GTTTGTGTTA GATCCGTGTT TGTGTTAGAT CCGTGCTGCT AGCGTTCGTA CACGGATGCG 22800

ACCTGTACGT CAGACACGTT CTGATTGCTA ACTTGCCAGT GTTTCTCTTT GGGGAATCCT 22860

GGGATGGCTC TAGCCGTTCC GCAGACGGGA TCGATTTCAT GATTTTTTTT GTTTCGTTGC 22920

ATAGGGTTTG GTTTGCCCTT TTCCTTTATT TCAATATATG CCGTGCACTT GTTTGTCGGG 22980

TCATCTTTTC ATGCTTTTTT TTGTCTTGGT TGTGATGATG TGGTCTGGTT GGGCGGTCGT 23040

TCTAGATCGG AGTAGAATTC TGTTTCAAAC TACCTGGTGG ATTTATTAAT TTTGGATCTG 23100

TATGTGTGTG CCATACATAT TCATAGTTAC GAATTGAAGA TGATGGATGG AAATATCGAT 23160

CTAGGATAGG TATACATGTT GATGCGGGTT TTACTGATGC ATATACAGAG ATGCTTTTTG 23220

TTCGCTTGGT TGTGATGATG TGGTGTGGTT GGGCGGTCGT TCATTCGTTC TAGATCGGAG 23280

TAGAATACTG TTTCAAACTA CCTGGTGTAT TTATTAATTT TGGAACTGTA TGTGTGTGTC 23340

ATACATCTTC ATAGTTACGA GTTTAAGATG GATGGAAATA TCGATCTAGG ATAGGTATAC 23400

ATGTTGATGT GGGTTTTACT GATGCATATA CATGATGGCA TATGCAGCAT CTATTCATAT 23460

GCTCTAACCT TGAGTACCTA TCTATTATAA TAAACAAGTA TGTTTTATAA TTATTTTGAT 23520

CTTGATATAC TTGGATGATG GCATATGCAG CAGCTATATG TGGATTTTTT TAGCCCTGCC 23580

TTCATACGCT ATTTATTTGC TTGGTACTGT TTCTTTTGTC GATGCTCACC CTGTTGTTTG 23640

GTGTTACTTC TGCAGGTCGA CTTTAACTTA GCCTAGGATC CACACGACAC CATGTCCCCC 23700

GAGCGCCGCC CCGTCGAGAT CCGCCCGGCC ACCGCCGCCG ACATGGCCGC CGTGTGCGAC 23760

ATCGTGAACC ACTACATCGA GACCTCCACC GTGAACTTCC GCACCGAGCC GCAGACCCCG 23820

CAGGAGTGGA TCGACGACCT GGAGCGCCTC CAGGACCGCT ACCCGTGGCT CGTGGCCGAG 23880

GTGGAGGGCG TGGTGGCCGG CATCGCCTAC GCCGGCCCGT GGAAGGCCCG CAACGCCTAC 23940

GACTGGACCG TGGAGTCCAC CGTGTACGTG TCCCACCGCC ACCAGCGCCT CGGCCTCGGC 24000

TCCACCCTCT ACACCCACCT CCTCAAGAGC ATGGAGGCCC AGGGCTTCAA GTCCGTGGTG 24060

GCCGTGATCG GCCTCCCGAA CGACCCGTCC GTGCGCCTCC ACGAGGCCCT CGGCTACACC 24120

GCCCGCGGCA CCCTCCGCGC CGCCGGCTAC AAGCACGGCG GCTGGCACGA CGTCGGCTTC 24180

TGGCAGCGCG ACTTCGAGCT GCCGGCCCCG CCGCGCCCGG TGCGCCCGGT GACGCAGATC 24240

TGAGTCGAAA CCTAGACTTG TCCATCTTCT GGATTGGCCA ACTTAATTAA TGTATGAAAT 24300

AAAAGGATGC ACACATAGTG ACATGCTAAT CACTATAATG TGGGCATCAA AGTTGTGTGT 24360

TATGTGTAAT TACTAGTTAT CTGAATAAAA GAGAAAGAGA TCATCCATAT TTCTTATCCT 24420

AAATGAATGT CACGTGTCTT TATAATTCTT TGATGAACCA GATGCATTTC ATTAACCAAA 24480

TCCATATACA TATAAATATT AATCATATAT AATTAATATC AATTGGGTTA GCAAAACAAA 24540

TCTAGTCTAG GTGTGTTTTG CGAATTGCGG CCGCCACCGC GGTGGAGCTC GAATTCATTC 24600

CGATTAATCG TGGCCTCTTG CTCTTCAGGA TGAAGAGCTA TGTTTAAACG TGCAAGCGCT 24660

ACTAGACAAT TCAGTACATT AAAAACGTCC GCAATGTGTT ATTAAGTTGT CTAAGCGTCA 24720

ATTTGTTTAC ACCACAATAT ATCCTGCCAC CAGCCAGCCA ACAGCTCCCC GACCGGCAGC 24780

TCGGCACAAA ATCACCACTC GATACAGGCA GCCCATCAGT CCGGGACGGC GTCAGCGGGA 24840

GAGCCGTTGT AAGGCGGCAG ACTTTGCTCA TGTTACCGAT GCTATTCGGA AGAACGGCAA 24900

CTAAGCTGCC GGGTTTGAAA CACGGATGAT CTCGCGGAGG GTAGCATGTT GATTGTAACG 24960

ATGACAGAGC GTTGCTGCCT GTGATCAAAT ATCATCTCCC TCGCAGAGAT CCGAATTATC 25020

AGCCTTCTTA TTCATTTCTC GCTTAACCGT GACAGGCTGT CGATCTTGAG AACTATGCCG 25080

ACATAATAGG AAATCGCTGG ATAAAGCCGC TGAGGAAGCT GAGTGGCGCT ATTTCTTTAG 25140

AAGTGAACGT TGACGATCGT CGACCGTACC CCGATGAATT AATTCGGACG TACGTTCTGA 25200

ACACAGCTGG ATACTTACTT GGGCGATTGT CATACATGAC ATCAACAATG TACCCGTTTG 25260

TGTAACCGTC TCTTGGAGGT TCGTATGACA CTAGTGGTTC CCCTCAGCTT GCGACTAGAT 25320

GTTGAGGCCT AACATTTTAT TAGAGAGCAG GCTAGTTGCT TAGATACATG ATCTTCAGGC 25380

CGTTATCTGT CAGGGCAAGC GAAAATTGGC CATTTATGAC GACCAATGCC CCGCAGAAGC 25440

TCCCATCTTT GCCGCCATAG ACGCCGCGCC CCCCTTTTGG GGTGTAGAAC ATCCTTTTGC 25500

CAGATGTGGA AAAGAAGTTC GTTGTCCCAT TGTTGGCAAT GACGTAGTAG CCGGCGAAAG 25560

TGCGAGACCC ATTTGCGCTA TATATAAGCC TACGATTTCC GTTGCGACTA TTGTCGTAAT 25620

TGGATGAACT ATTATCGTAG TTGCTCTCAG AGTTGTCGTA ATTTGATGGA CTATTGTCGT 25680

AATTGCTTAT GGAGTTGTCG TAGTTGCTTG GAGAAATGTC GTAGTTGGAT GGGGAGTAGT 25740

CATAGGGAAG ACGAGCTTCA TCCACTAAAA CAATTGGCAG GTCAGCAAGT GCCTGCCCCG 25800

ATGCCATCGC AAGTACGAGG CTTAGAACCA CCTTCAACAG ATCGCGCATA GTCTTCCCCA 25860

GCTCTCTAAC GCTTGAGTTA AGCCGCGCCG CGAAGCGGCG TCGGCTTGAA CGAATTGTTA 25920

GACATTATTT GCCGACTACC TTGGTGATCT CGCCTTTCAC GTAGTGAACA AATTCTTCCA 25980

ACTGATCTGC GCGCGAGGCC AAGCGATCTT CTTGTCCAAG ATAAGCCTGC CTAGCTTCAA 26040

GTATGACGGG CTGATACTGG GCCGGCAGGC GCTCCATTGC CCAGTCGGCA GCGACATCCT 26100

TCGGCGCGAT TTTGCCGGTT ACTGCGCTGT ACCAAATGCG GGACAACGTA AGCACTACAT 26160

TTCGCTCATC GCCAGCCCAG TCGGGCGGCG AGTTCCATAG CGTTAAGGTT TCATTTAGCG 26220

CCTCAAATAG ATCCTGTTCA GGAACCGGAT CAAAGAGTTC CTCCGCCGCT GGACCTACCA 26280AGGCAACGCT ATGTTCTCTT GCTTTTGTCA GCAAGATAGC CAGATCAATG TCGATCGTGG 26340

CTGGCTCGAA GATACCTGCA AGAATGTCAT TGCGCTGCCA TTCTCCAAAT TGCAGTTCGC 26400

GCTTAGCTGG ATAACGCCAC GGAATGATGT CGTCGTGCAC AACAATGGTG ACTTCTACAG 264 60

CGCGGAGAAT CTCGCTCTCT CCAGGGGAAG CCGAAGTTTC CAAAAGGTCG TTGATCAAAG 26520

CTCGCCGCGT TGTTTCATCA AGCCTTACAG TCACCGTAAC CAGCAAATCA ATATCACTGT 26580

GTGGCTTCAG GCCGCCATCC ACTGCGGAGC CGTACAAATG TACGGCCAGC AACGTCGGTT 26640

CGAGATGGCG CTCGATGACG CCAACTACCT CTGATAGTTG AGTCGATACT TCGGCGATCA 26700

CCGCTTCCCT CATGATGTTT AACTCCTGAA TTAAGCCGCG CCGCGAAGCG GTGTCGGCTT 267 60

GAATGAATTG TTAGGCGTCA TCCTGTGCTC CCGAGAACCA GTACCAGTAC ATCGCTGTTT 26820

CGTTCGAGAC TTGAGGTCTA GTTTTATACG TGAACAGGTC AATGCCGCCG AGAGTAAAGC 26880

CACATTTTGC GTACAAATTG CAGGCAGGTA CATTGTTCGT TTGTGTCTCT AATCGTATGC 2694 0

CAAGGAGCTG TCTGCTTAGT GCCCACTTTT TCGCAAATTC GATGAGACTG TGCGCGACTC 27000

CTTTGCCTCG GTGCGTGTGC GACACAACAA TGTGTTCGAT AGAGGCTAGA TCGTTCCATG 27060

TTGAGTTGAG TTCAATCTTC CCGACAAGCT CTTGGTCGAT GAATGCGCCA TAGCAAGCAG 27120

AGTCTTCATC AGAGTCATCA TCCGAGATGT AATCCTTCCG GTAGGGGCTC ACACTTCTGG 27180

TAGATAGTTC AAAGCCTTGG TCGGATAGGT GCACATCGAA CACTTCACGA ACAATGAAAT 2724 0

GGTTCTCAGC ATCCAATGTT TCCGCCACCT GCTCAGGGAT CACCGAAATC TTCATATGAC 27 300

GCCTAACGCC TGGCACAGCG GATCGCAAAC CTGGCGCGGC TTTTGGCACA AAAGGCGTGA 27360

CAGGTTTGCG AATCCGTTGC TGCCACTTGT TAACCCTTTT GCCAGATTTG GTAACTATAA 27 4 20

TTTATGTTAG AGGCGAAGTC TTGGGTAAAA ACTGGCCTAA AATTGCTGGG GATTTCAGGA 274 80

AAGTAAACAT CACCTTCCGG CTCGATGTCT ATTGTAGATA TATGTAGTGT ATCTACTTGA 27 540

TCGGGGGATC TGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA 27 600

GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 27660

GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA 27 720

TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 277 80

CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT 2784 0

TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA 27 900

GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 27 960

ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 28020

TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 28080

CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 28140

TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 28200

GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 28260

AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 28320

TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 28380

AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 28440

AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 28500

TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 28560

TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 28 620

ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 28 680

ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 2874 0

TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 28800

GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 28860

TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 28 920

GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 28 980

CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 29040

GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTGCAGGG 29100

GGGGGGGGGG GGGGGGACTT CCATTGTTCA TTCCACGGAC AAAAACAGAG AAAGGAAACG 29160

ACAGAGGCCA AAAAGCCTCG CTTTCAGCAC CTGTCGTTTC CTTTCTTTTC AGAGGGTATT 29220

TTAAATAAAA ACATTAAGTT ATGACGAAGA AGAACGGAAA CGCCTTAAAC CGGAAAATTT 29280

TCATAAATAG CGAAAACCCG CGAGGTCGCC GCCCCGTAAG CCGCCCCGTA ACCTGTCGGA 2934 0

TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT ACATATCACA ACGTGCGTGG 29400

AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG TATCGTATTA ATTGATCTGC 294 60

ATCAACTTAA CGTAAAAACA ACTTCAGACA ATACAAATCA GCGACACTGA ATACGGGGCA 29520

ACCTCATGTC CCCCCCCCCC CCCCCCCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 29580

TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 29640

GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 29700

AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 29760

AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 29820GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 29880

TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 29940

GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 30000

TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 30060

AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 30120

CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 30180

ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 3024 0

TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAAGA 30300

ATTCGGAGCT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG 30360

ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA 30420

TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT 30480

CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC 30540

AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT 30600

GGCAGAGCAT TACGCTGACT TGACGGGACG GCGGCTTTGT TGAATAAATC GAACTTTTGC 30 660

TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA 30720

AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT 307 80

CACTTTCTGG CTGGATGATG GGGCGATTCA GGCCTGGTAT GAGTCAGCAA CACCTTCTTC 3084 0

ACGAGGCAGA CCTCAGCGCC AGAAGGCCGC CAGAGAGGCC GAGCGCGGCC GTGAGGCTTG 30900

GACGCTAGGG CAGGGCATGA AAAAGCCCGT AGCGGGCTGC TACGGGCGTC TGACGCGGTG 30960

GAAAGGGGGA GGGGATGTTG TCTACATGGC TCTGCTGTAG TGAGTGGGTT GCGCTCCGGC 31020

AGCGGTCCTG ATCAATCGTC ACCCTTTCTC GGTCCTTCAA CGTTCCTGAC AACGAGCCTC 31080

CTTTTCGCCA ATCCATCGAC AATCACCGCG AGTCCCTGCT CGAACGCTGC GTCCGGACCG 3114 0

GCTTCGTCGA AGGCGTCTAT CGCGGCCCGC AACAGCGGCG AGAGCGGAGC CTGTTCAACG 31200

GTGCCGCCGC GCTCGCCGGC ATCGCTGTCG CCGGCCTGCT CCTCAAGCAC GGCCCCAACA 31260

GTGAAGTAGC TGATTGTCAT CAGCGCATTG ACGGCGTCCC CGGCCGAAAA ACCCGCCTCG 31320

CAGAGGAAGC GAAGCTGCGC GTCGGCCGTT TCCATCTGCG GTGCGCCCGG TCGCGTGCCG 31380

GCATGGATGC GCGCGCCATC GCGGTAGGCG AGCAGCGCCT GCCTGAAGCT GCGGGCATTC 314 4 0

CCGATCAGAA ATGAGCGCCA GTCGTCGTCG GCTCTCGGCA CCGAATGCGT ATGATTCTCC 31500

GCCAGCATGG CTTCGGCCAG TGCGTCGAGC AGCGCCCGCT TGTTCCTGAA GTGCCAGTAA 31560

AGCGCCGGCT GCTGAACCCC CAACCGTTCC GCCAGTTTGC GTGTCGTCAG ACCGTCTACG 31620

CCGACCTCGT TCAACAGGTC CAGGGCGGCA CGGATCACTG TATTCGGCTG CAACTTTGTC 31680

ATGCTTGACA CTTTATCACT GATAAACATA ATATGTCCAC CAACTTATCA GTGATAAAGA 3174 0

ATCCGCGCGT TCAATCGGAC CAGCGGAGGC TGGTCCGGAG GCCAGACGTG AAACCCAACA 31800

TACCCCTGAT CGTAATTCTG AGCACTGTCG CGCTCGACGC TGTCGGCATC GGCCTGATTA 31860

TGCCGGTGCT GCCGGGCCTC CTGCGCGATC TGGTTCACTC GAACGACGTC ACCGCCCACT 31920

ATGGCATTCT GCTGGCGCTG TATGCGTTGG TGCAATTTGC CTGCGCACCT GTGCTGGGCG 31980

CGCTGTCGGA TCGTTTCGGG CGGCGGCCAA TCTTGCTCGT CTCGCTGGCC GGCGCCACTG 32040

TCGACTACGC CATCATGGCG ACAGCGCCTT TCCTTTGGGT TCTCTATATC GGGCGGATCG 32100

TGGCCGGCAT CACCGGGGCG ACTGGGGCGG TAGCCGGCGC TTATATTGCC GATATCACTG 32160

ATGGCGATGA GCGCGCGCGG CACTTCGGCT TCATGAGCGC CTGTTTCGGG TTCGGGATGG 32220

TCGCGGGACC TGTGCTCGGT GGGCTGATGG GCGGTTTCTC CCCCCACGCT CCGTTCTTCG 32280

CCGCGGCAGC CTTGAACGGC CTCAATTTCC TGACGGGCTG TTTCCTTTTG CCGGAGTCGC 3234 0

ACAAAGGCGA ACGCCGGCCG TTACGCCGGG AGGCTCTCAA CCCGCTCGCT TCGTTCCGGT 32400

GGGCCCGGGG CATGACCGTC GTCGCCGCCC TGATGGCGGT CTTCTTCATC ATGCAACTTG 324 60

TCGGACAGGT GCCGGCCGCG CTTTGGGTCA TTTTCGGCGA GGATCGCTTT CACTGGGACG 32520

CGACCACGAT CGGCATTTCG CTTGCCGCAT TTGGCATTCT GCATTCACTC GCCCAGGCAA 32580

TGATCACCGG CCCTGTAGCC GCCCGGCTCG GCGAAAGGCG GGCACTCATG CTCGGAATGA 32 640

TTGCCGACGG CACAGGCTAC ATCCTGCTTG CCTTCGCGAC ACGGGGATGG ATGGCGTTCC 32700

CGATCATGGT CCTGCTTGCT TCGGGTGGCA TCGGAATGCC GGCGCTGCAA GCAATGTTGT 32760

CCAGGCAGGT GGATGAGGAA CGTCAGGGGC AGCTGCAAGG CTCACTGGCG GCGCTCACCA 32820

GCCTGACCTC GATCGTCGGA CCCCTCCTCT TCACGGCGAT CTATGCGGCT TCTATAACAA 32880

CGTGGAACGG GTGGGCATGG ATTGCAGGCG CTGCCCTCTA CTTGCTCTGC CTGCCGGCGC 32 94 0

TGCGTCGCGG GCTTTGGAGC GGCGCAGGGC AACGAGCCGA TCGCTGATCG TGGAAACGAT 33000

AGGCCTATGC CATGCGGGTC AAGGCGACTT CCGGCAAGCT ATACGCGCCC TAGGAGTGCG 33060

GTTGGAACGT TGGCCCAGCC AGATACTCCC GATCACGAGC AGGACGCCGA TGATTTGAAG 33120

CGCACTCAGC GTCTGATCCA AGAACAACCA TCCTAGCAAC ACGGCGGTCC CCGGGCTGAG 33180

AAAGCCCAGT AAGGAAACAA CTGTAGGTTC GAGTCGCGAG ATCCCCCGGA ACCAAAGGAA 33240

GTAGGTTAAA CCCGCTCCGA TCAGGCCGAG CCACGCCAGG CCGAGAACAT TGGTTCCTGT 33300

AGGCATCGGG ATTGGCGGAT CAAACACTAA AGCTACTGGA ACGAGCAGAA GTCCTCCGGC 33360CGCCAGTTGC CAGGCGGTAA AGGTGAGCAG AGGCACGGGA GGTTGCCACT TGCGGGTCAG 33420

CACGGTTCCG AACGCCATGG AAACCGCCCC CGCCAGGCCC GCTGCGACGC CGACAGGATC 334 80

TAGCGCTGCG TTTGGTGTCA ACACCAACAG CGCCACGCCC GCAGTTCCGC AAATAGCCCC 33540

CAGGACCGCC ATCAATCGTA TCGGGCTACC TAGCAGAGCG GCAGAGATGA ACACGACCAT 33600

CAGCGGCTGC ACAGCGCCTA CCGTCGCCGC GACCCCGCCC GGCAGGCGGT AGACCGAAAT 33660

AAACAACAAG CTCCAGAATA GCGAAATATT AAGTGCGCCG AGGATGAAGA TGCGCATCCA 33720

CCAGATTCCC GTTGGAATCT GTCGGACGAT CATCACGAGC AATAAACCCG CCGGCAACGC 33780

CCGCAGCAGC ATACCGGCGA CCCCTCGGCC TCGCTGTTCG GGCTCCACGA AAACGCCGGA 3384 0

CAGATGCGCC TTGTGAGCGT CCTTGGGGCC GTCCTCCTGT TTGAAGACCG ACAGCCCAAT 33900

GATCTCGCCG TCGATGTAGG CGCCGAATGC CACGGCATCT CGCAACCGTT CAGCGAACGC 33960

CTCCATGGGC TTTTTCTCCT CGTGCTCGTA AACGGACCCG AACATCTCTG GAGCTTTCTT 34020

CAGGGCCGAC AATCGGATCT CGCGGAAATC CTGCACGTCG GCCGCTCCAA GCCGTCGAAT 34 080

CTGAGCCTTA ATCACAATTG TCAATTTTAA TCCTCTGTTT ATCGGCAGTT CGTAGAGCGC 3414 0

GCCGTGCGTC CCGAGCGATA CTGAGCGAAG CAAGTGCGTC GAGCAGTGCC CGCTTGTTCC 34200

TGAAATGCCA GTAAAGCGCT GGCTGCTGAA CCCCCAGCCG GAACTGACCC CACAAGGCCC 34260

TAGCGTTTGC AATGCACCAG GTCATCATTG ACCCAGGCGT GTTCCACCAG GCCGCTGCCT 34 320

CGCAACTCTT CGCAGGCTTC GCCGACCTGC TCGCGCCACT TCTTCACGCG GGTGGAATCC 34380

GATCCGCACA TGAGGCGGAA GGTTTCCAGC TTGAGCGGGT ACGGCTCCCG GTGCGAGCTG 34 4 40

AAATAGTCGA ACATCCGTCG GGCCGTCGGC GACAGCTTGC GGTACTTCTC CCATATGAAT 34 500

TTCGTGTAGT GGTCGCCAGC AAACAGCACG ACGATTTCCT CGTCGATCAG GACCTGGCAA 34 560

CGGGACGTTT TCTTGCCACG GTCCAGGACG CGGAAGCGGT GCAGCAGCGA CACCGATTCC 34 620

AGGTGCCCAA CGCGGTCGGA CGTGAAGCCC ATCGCCGTCG CCTGTAGGCG CGACAGGCAT 34 680

TCCTCGGCCT TCGTGTAATA CCGGCCATTG ATCGACCAGC CCAGGTCCTG GCAAAGCTCG 34740

TAGAACGTGA AGGTGATCGG CTCGCCGATA GGGGTGCGCT TCGCGTACTC CAACACCTGC 34800

TGCCACACCA GTTCGTCATC GTCGGCCCGC AGCTCGACGC CGGTGTAGGT GATCTTCACG 34860

TCCTTGTTGA CGTGGAAAAT GACCTTGTTT TGCAGCGCCT CGCGCGGGAT TTTCTTGTTG 34 920

CGCGTGGTGA ACAGGGCAGA GCGGGCCGTG TCGTTTGGCA TCGCTCGCAT CGTGTCCGGC 34 980

CACGGCGCAA TATCGAACAA GGAAAGCTGC ATTTCCTTGA TCTGCTGCTT CGTGTGTTTC 35040

AGCAACGCGG CCTGCTTGGC CTCGCTGACC TGTTTTGCCA GGTCCTCGCC GGCGGTTTTT 35100

CGCTTCTTGG TCGTCATAGT TCCTCGCGTG TCGATGGTCA TCGACTTCGC CAAACCTGCC 35160

GCCTCCTGTT CGAGACGACG CGAACGCTCC ACGGCGGCCG ATGGCGCGGG CAGGGCAGGG 35220

GGAGCCAGTT GCACGCTGTC GCGCTCGATC TTGGCCGTAG CTTGCTGGAC CATCGAGCCG 35280

ACGGACTGGA AGGTTTCGCG GGGCGCACGC ATGACGGTGC GGCTTGCGAT GGTTTCGGCA 3534 0

TCCTCGGCGG AAAACCCCGC GTCGATCAGT TCTTGCCTGT ATGCCTTCCG GTCAAACGTC 354 00

CGATTCATTC ACCCTCCTTG CGGGATTGCC CCGACTCACG CCGGGGCAAT GTGCCCTTAT 354 60

TCCTGATTTG ACCCGCCTGG TGCCTTGGTG TCCAGATAAT CCACCTTATC GGCAATGAAG 35520

TCGGTCCCGT AGACCGTCTG GCCGTCCTTC TCGTACTTGG TATTCCGAAT CTTGCCCTGC 35580

ACGAATACCA GCGACCCCTT GCCCAAATAC TTGCCGTGGG CCTCGGCCTG AGAGCCAAAA 3564 0

CACTTGATGC GGAAGAAGTC GGTGCGCTCC TGCTTGTCGC CGGCATCGTT GCGCCACTCT 35700

TCATTAACCG CTATATCGAA AATTGCTTGC GGCTTGTTAG AATTGCCATG ACGTACCTCG 357 60

GTGTCACGGG TAAGATTACC GATAAACTGG AACTGATTAT GGCTCATATC GAAAGTCTCC 35820

TTGAGAAAGG AGACTCTAGT TTAGCTAAAC ATTGGTTCCG CTGTCAAGAA CTTTAGCGGC 35880

TAAAATTTTG CGGGCCGCGA CCAAAGGTGC GAGGGGCGGC TTCCGCTGTG TACAACCAGA 35940

TATTTTTCAC CAACATCCTT CGTCTGCTCG ATGAGCGGGG CATGACGAAA CATGAGCTGT 36000

CGGAGAGGGC AGGGGTTTCA ATTTCGTTTT TATCAGACTT AACCAACGGT AAGGCCAACC 36060

CCTCGTTGAA GGTGATGGAG GCCATTGCCG ACGCCCTGGA AACTCCCCTA CCTCTTCTCC 36120

TGGAGTCCAC CGACCTTGAC CGCGAGGCAC TCGCGGAGAT TGCGGGTCAT CCTTTCAAGA 36180

GCAGCGTGCC GCCCGGATAC GAACGCATCA GTGTGGTTTT GCCGTCACAT AAGGCGTTTA 36240

TCGTAAAGAA ATGGGGCGAC GACACCCGAA AAAAGCTGCG TGGAAGGCTC TGACGCCAAG 36300

GGTTAGGGCT TGCACTTCCT TCTTTAGCCG CTAAAACGGC CCCTTCTCTG CGGGCCGTCG 36360

GCTCGCGCAT CATATCGACA TCCTCAACGG AAGCCGTGCC GCGAATGGCA TCGGGCGGGT 36420

GCGCTTTGAC AGTTGTTTTC TATCAGAACC CCTACGTCGT GCGGTTCGAT TAGCTGTTTG 36480

TCTTGCAGGC TAAACACTTT CGGTATATCG TTTGCCTGTG CGATAATGTT GCTAATGATT 36540

TGTTGCGTAG GGGTTACTGA AAAGTGAGCG GGAAAGAAGA GTTTCAGACC ATCAAGGAGC 36600

GGGCCAAGCG CAAGCTGGAA CGCGACATGG GTGCGGACCT GTTGGCCGCG CTCAACGACC 36660

CGAAAACCGT TGAAGTCATG CTCAACGCGG ACGGCAAGGT GTGGCACGAA CGCCTTGGCG 36720

AGCCGATGCG GTACATCTGC GACATGCGGC CCAGCCAGTC GCAGGCGATT ATAGAAACGG 36780

TGGCCGGATT CCACGGCAAA GAGGTCACGC GGCATTCGCC CATCCTGGAA GGCGAGTTCC 36840

CCTTGGATGG CAGCCGCTTT GCCGGCCAAT TGCCGCCGGT CGTGGCCGCG CCAACCTTTG 36900CGATCCGCAA GCGCGCGGTC GCCATCTTCA CGCTGGAACA GTACGTCGAG GCGGGCATCA 36960

TGACCCGCGA GCAATACGAG GTCATTAAAA GCGCCGTCGC GGCGCATCGA AACATCCTCG 37020

TCATTGGCGG TACTGGCTCG GGCAAGACCA CGCTCGTCAA CGCGATCATC AATGAAATGG 37080

TCGCCTTCAA CCCGTCTGAG CGCGTCGTCA TCATCGAGGA CACCGGCGAA ATCCAGTGCG 37140

CCGCAGAGAA CGCCGTCCAA TACCACACCA GCATCGACGT CTCGATGACG CTGCTGCTCA 37200

AGACAACGCT GCGTATGCGC CCCGACCGCA TCCTGGTCGG TGAGGTACGT GGCCCCGAAG 372 60

CCCTTGATCT GTTGATGGCC TGGAACACCG GGCATGAAGG AGGTGCCGCC ACCCTGCACG 37320

CAAACAACCC CAAAGCGGGC CTGAGCCGGC TCGCCATGCT TATCAGCATG CACCCGGATT 37380

CACCGAAACC CATTGAGCCG CTGATTGGCG AGGCGGTTCA TGTGGTCGTC CATATCGCCA 374 40

GGACCCCTAG CGGCCGTCGA GTGCAAGAAA TTCTCGAAGT TCTTGGTTAC GAGAACGGCC 37 500

AGTACATCAC CAAAACCCTG TAAGGAGTAT TTCCAATGAC AACGGCTGTT CCGTTCCGTC 37560

TGACCATGAA TCGCGGCATT TTGTTCTACC TTGCCGTGTT CTTCGTTCTC GCTCTCGCGT 37 620

TATCCGCGCA TCCGGCGATG GCCTCGGAAG GCACCGGCGG CAGCTTGCCA TATGAGAGCT 37 680

GGCTGACGAA CCTGCGCAAC TCCGTAACCG GCCCGGTGGC CTTCGCGCTG TCCATCATCG 3774 0

GCATCGTCGT CGCCGGCGGC GTGCTGATCT TCGGCGGCGA ACTCAACGCC TTCTTCCGAA 37800

CCCTGATCTT CCTGGTTCTG GTGATGGCGC TGCTGGTCGG CGCGCAGAAC GTGATGAGCA 37 860

CCTTCTTCGG TCGTGGTGCC GAAATCGCGG CCCTCGGCAA CGGGGCGCTG CACCAGGTGC 37 920

AAGTCGCGGC GGCGGATGCC GTGCGTGCGG TAGCGGCTGG ACGGCTCGCC TAATCATGGC 37 980

TCTGCGCACG ATCCCCATCC GTCGCGCAGG CAACCGAGAA AACCTGTTCA TGGGTGGTGA 3804 0

TCGTGAACTG GTGATGTTCT CGGGCCTGAT GGCGTTTGCG CTGATTTTCA GCGCCCAAGA 38100

GCTGCGGGCC ACCGTGGTCG GTCTGATCCT GTGGTTCGGG GCGCTCTATG CGTTCCGAAT 38160

CATGGCGAAG GCCGATCCGA AGATGCGGTT CGTGTACCTG CGTCACCGCC GGTACAAGCC 38220

GTATTACCCG GCCCGCTCGA CCCCGTTCCG CGAGAACACC AATAGCCAAG GGAAGCAATA 38280

CCGATGATCC AAGCAATTGC GATTGCAATC GCGGGCCTCG GCGCGCTTCT GTTGTTCATC 38340

CTCTTTGCCC GCATCCGCGC GGTCGATGCC GAACTGAAAC TGAAAAAGCA TCGTTCCAAG 38400

GACGCCGGCC TGGCCGATCT GCTCAACTAC GCCGCTGTCG TCGATGACGG CGTAATCGTG 384 60

GGCAAGAACG GCAGCTTTAT GGCTGCCTGG CTGTACAAGG GCGATGACAA CGCAAGCAGC 38520

ACCGACCAGC AGCGCGAAGT AGTGTCCGCC CGCATCAACC AGGCCCTCGC GGGCCTGGGA 38580

AGTGGGTGGA TGATCCATGT GGACGCCGTG CGGCGTCCTG CTCCGAACTA CGCGGAGCGG 38 64 0

GGCCTGTCGG CGTTCCCTGA CCGTCTGACG GCAGCGATTG AAGAAGAGCG CTCGGTCTTG 38700

CCTTGCTCGT CGGTGATGTA CTTCACCAGC TCCGCGAAGT CGCTCTTCTT GATGGAGCGC 387 60

ATGGGGACGT GCTTGGCAAT CACGCGCACC CCCCGGCCGT TTTAGCGGCT AAAAAAGTCA 38820

TGGCTCTGCC CTCGGGCGGA CCACGCCCAT CATGACCTTG CCAAGCTCGT CCTGCTTCTC 38880

TTCGATCTTC GCCAGCAGGG CGAGGATCGT GGCATCACCG AACCGCGCCG TGCGCGGGTC 38 94 0

GTCGGTGAGC CAGAGTTTCA GCAGGCCGCC CAGGCGGCCC AGGTCGCCAT TGATGCGGGC 39000

CAGCTCGCGG ACGTGCTCAT AGTCCACGAC GCCCGTGATT TTGTAGCCCT GGCCGACGGC 39060

CAGCAGGTAG GCCGACAGGC TCATGCCGGC CGCCGCCGCC TTTTCCTCAA TCGCTCTTCG 39120

TTCGTCTGGA AGGCAGTACA CCTTGATAGG TGGGCTGCCC TTCCTGGTTG GCTTGGTTTC 39180

ATCAGCCATC CGCTTGCCCT CATCTGTTAC GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC 39240

AGGATTCCCG TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT 39300

CGCGGGTGGG CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA 39360

GTCTACACGA ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA 39420

AAAAATCGCT ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAAGCGCT GCTTCCCTGC 39480

TGTTTTGTGG AATATCTACC GACTGGAAAC AGGCAAATGC AGGAAATTAC TGAACTGAGG 3954 0

GGACAGGCGA GAGACGATGC CAAAGAGCTA CACCGACGAG CTGGCCGAGT GGGTTGAATC 39600

CCGCGCGGCC AAGAAGCGCC GGCGTGATGA GGCTGCGGTT GCGTTCCTGG CGGTGAGGGC 39660

GGATGTCGAG GCGGCGTTAG CGTCCGGCTA TGCGCTCGTC ACCATTTGGG AGCACATGCG 39720

GGAAACGGGG AAGGTCAAGT TCTCCTACGA GACGTTCCGC TCGCACGCCA GGCGGCACAT 39780

CAAGGCCAAG CCCGCCGATG TGCCCGCACC GCAGGCCAAG GCTGCGGAAC CCGCGCCGGC 39840

ACCCAAGACG CCGGAGCCAC GGCGGCCGAA GCAGGGGGGC AAGGCTGAAA AGCCGGCCCC 39900

CGCTGCGGCC CCGACCGGCT TCACCTTCAA CCCAACACCG GACAAAAAGG ATCTACTGTA 39960

ATGGCGAAAA TTCACATGGT TTTGCAGGGC AAGGGCGGGG TCGGCAAGTC GGCCATCGCC 40020

GCGATCATTG CGCAGTACAA GATGGACAAG GGGCAGACAC CCTTGTGCAT CGACACCGAC 40080

CCGGTGAACG CGACGTTCGA GGGCTACAAG GCCCTGAACG TCCGCCGGCT GAACATCATG 4 014 0

GCCGGCGACG AAATTAACTC GCGCAACTTC GACACCCTGG TCGAGCTGAT TGCGCCGACC 4 0200

AAGGATGACG TGGTGATCGA CAACGGTGCC AGCTCGTTCG TGCCTCTGTC GCATTACCTC 4 0260

ATCAGCAACC AGGTGCCGGC TCTGCTGCAA GAAATGGGGC ATGAGCTGGT CATCCATACC 4 0320

GTCGTCACCG GCGGCCAGGC TCTCCTGGAC ACGGTGAGCG GCTTCGCCCA GCTCGCCAGC 40380

CAGTTCCCGG CCGAAGCGCT TTTCGTGGTC TGGCTGAACC CGTATTGGGG GCCTATCGAG 4 0440CATGAGGGCA AGAGCTTTGA GCAGATGAAG GCGTACACGG CCAACAAGGC CCGCGTGTCG 4 0500

TCCATCATCC AGATTCCGGC CCTCAAGGAA GAAACCTACG GCCGCGATTT CAGCGACATG 4 0560

CTGCAAGAGC GGCTGACGTT CGACCAGGCG CTGGCCGATG AATCGCTCAC GATCATGACG 4 0620

CGGCAACGCC TCAAGATCGT GCGGCGCGGC CTGTTTGAAC AGCTCGACGC GGCGGCCGTG 40680

CTATGAGCGA CCAGATTGAA GAGCTGATCC GGGAGATTGC GGCCAAGCAC GGCATCGCCG 4 0740

TCGGCCGCGA CGACCCGGTG CTGATCCTGC ATACCATCAA CGCCCGGCTC ATGGCCGACA 4 0800

GTGCGGCCAA GCAAGAGGAA ATCCTTGCCG CGTTCAAGGA AGAGCTGGAA GGGATCGCCC 4 0860

ATCGTTGGGG CGAGGACGCC AAGGCCAAAG CGGAGCGGAT GCTGAACGCG GCCCTGGCGG 4 0920

CCAGCAAGGA CGCAATGGCG AAGGTAATGA AGGACAGCGC CGCGCAGGCG GCCGAAGCGA 40980

TCCGCAGGGA AATCGACGAC GGCCTTGGCC GCCAGCTCGC GGCCAAGGTC GCGGACGCGC 41040

GGCGCGTGGC GATGATGAAC ATGATCGCCG GCGGCATGGT GTTGTTCGCG GCCGCCCTGG 41100

TGGTGTGGGC CTCGTTATGA ATCGCAGAGG CGCAGATGAA AAAGCCCGGC GTTGCCGGGC 41160

TTTGTTTTTG CGTTAGCTGG GCTTGTTTGA CAGGCCCAAG CTCTGACTGC GCCCGCGCTC 41220

GCGCTCCTGG GCCTGTTTCT TCTCCTGCTC CTGCTTGCGC ATCAGGGCCT GGTGCCGTCG 41280

GGCTGCTTCA CGCATCGAAT CCCAGTCGCC GGCCAGCTCG GGATGCTCCG CGCGCATCTT 41340

GCGCGTCGCC AGTTCCTCGA TCTTGGGCGC GTGAATGCCC ATGCCTTCCT TGATTTCGCG 41400

CACCATGTCC AGCCGCGTGT GCAGGGTCTG CAAGCGGGCT TGCTGTTGGG CCTGCTGCTG 414 60

CTGCCAGGCG GCCTTTGTAC GCGGCAGGGA CAGCAAGCCG GGGGCATTGG ACTGTAGCTG 41520

CTGCAAACGC GCCTGCTGAC GGTCTACGAG CTGTTCTAGG CGGTCCTCGA TGCGCTCCAC 41580

CTGGTCATGC TTTGCCTGCA CGTAGAGCGC AAGGGTCTGC TGGTAGGTCT GCTCGATGGG 41640

CGCGGATTCT AAGAGGGCCT GCTGTTCCGT CTCGGCCTCC TGGGCCGCCT GTAGCAAATC 41700

CTCGCCGCTG TTGCCGCTGG ACTGCTTTAC TGCCGGGGAC TGCTGTTGCC CTGCTCGCGC 41760

CGTCGTCGCA GTTCGGCTTG CCCCCACTCG ATTGACTGCT TCATTTCGAG CCGCAGCGAT 41820

GCGATCTCGG ATTGCGTCAA CGGACGGGGC AGCGCGGAGG TGTCCGGCTT CTCCTTGGGT 41880

GAGTCGGTCG ATGCCATAGC CAAAGGTTTC CTTCCAAAAT GCGTCCATTG CTGGACCGTG 41940

TTTCTCATTG ATGCCCGCAA GCATCTTCGG CTTGACCGCC AGGTCAAGCG CGCCTTCATG 4 2000

GGCGGTCATG ACGGACGCCG CCATGACCTT GCCGCCGTTG TTCTCGATGT AGCCGCGTAA 4 2060

TGAGGCAATG GTGCCGCCCA TCGTCAGCGT GTCATCGACA ACGATGTACT TCTGGCCGGG 4 2120

GATCACCTCC CCCTCGAAAG TCGGGTTGAA CGCCAGGCGA TGATCTGAAC CGGCTCCGGT 4 2180

TCGGGCGACC TTCTCCCGCT GCACAATGTC CGTTTCGACC TCAAGGCCAA GGCGGTCGGC 42240

CAGAACGACC GCCATCATGG CCGGAATCTT GTTGTTCCCC GCCGCCTCGA CGGCGAGGAC 4 2300

TGGAACGATG CGGGGCTTGT CGTCGCCGAT CAGCGTCTTG AGCTGGGCAA CAGTGTCGTC 4 2360

CGAAATCAGG CGCTCGACCA AATTAAGCGC CGCTTCCGCG TCGCCCTGCT TCGCAGCCTG 42420

GTATTCAGGC TCGTTGGTCA AAGAACCAAG GTCGCCGTTG CGAACCACCT TCGGGAAGTC 4 24 80

TCCCCACGGT GCGCGCTCGG CTCTGCTGTA GCTGCTCAAG ACGCCTCCCT TTTTAGCCGC 42540

TAAAACTCTA ACGAGTGCGC CCGCGACTCA ACTTGACGCT TTCGGCACTT ACCTGTGCCT 4 2 600

TGCCACTTGC GTCATAGGTG ATGCTTTTCG CACTCCCGAT TTCAGGTACT TTATCGAAAT 4 2 660

CTGACCGGGC GTGCATTACA AAGTTCTTCC CCACCTGTTG GTAAATGCTG CCGCTATCTG 42720

CGTGGACGAT GCTGCCGTCG TGGCGCTGCG ACTTATCGGC CTTTTGGGCC ATATAGATGT 4 2780

TGTAAATGCC AGGTTTCAGG GCCCCGGCTT TATCTACCTT CTGGTTCGTC CATGCGCCTT 42840

GGTTCTCGGT CTGGACAATT CTTTGCCCAT TCATGACCAG GAGGCGGTGT TTCATTGGGT 42900

GACTCCTGAC GGTTGCCTCT GGTGTTAAAC GTGTCCTGGT CGCTTGCCGG CTAAAAAAAA 42960GCCGACCTCG GCAGTTCGAG GCCGGCTTTC CCTAGAGCCG GGCGCGTCAA GGTTGTTCCA 4 3020

TCTATTTTAG TGAACTGCGT TCGATTTATC AGTTACTTTC CTCCCGCTTT GTGTTTCCTC 4 3080

CCACTCGTTT CCGCGTCTAG CCGACCCCTC AACATAGCGG CCTCTTCTTG GGCTGCCTTT 43140

GCCTCTTGCC GCGCTTCGTC ACGCTCGGCT TGCACCGTCG TAAAGCGCTC GGCCTGCCTG 4 3200

GCCGCCTCTT GCGCCGCCAA CTTCCTTTGC TCCTGGTGGG CCTCGGCGTC GGCCTGCGCC 4 3260

TTCGCTTTCA CCGCTGCCAA CTCCGTGCGC AAACTCTCCG CTTCGCGCCT GGTGGCGTCG 43320

CGCTCGCCGC GAAGCGCCTG CATTTCCTGG TTGGCCGCGT CCAGGGTCTT GCGGCTCTCT 4 3380

TCTTTGAATG CGCGGGCGTC CTGGTGAGCG TAGTCCAGCT CGGCGCGCAG CTCCTGCGCT 4 3440

CGACGCTCCA CCTCGTCGGC CCGCTGCGTC GCCAGCGCGG CCCGCTGCTC GGCTCCTGCC 4 3500

AGGGCGGTGC GTGCTTCGGC CAGGGCTTGC CGCTGGCGTG CGGCCAGCTC GGCCGCCTCG 4 3560

GCGGCCTGCT GCTCTAGCAA TGTAACGCGC GCCTGGGCTT CTTCCAGCTC GCGGGCCTGC 4 3620

GCCTCGAAGG CGTCGGCCAG CTCCCCGCGC ACGGCTTCCA ACTCGTTGCG CTCACGATCC 43680

CAGCCGGCTT GCGCTGCCTG CAACGATTCA TTGGCAAGGG CCTGGGCGGC TTGCCAGAGG 43740

GCGGCCACGG CCTGGTTGCC GGCCTGCTGC ACCGCGTCCG GCACCTGGAC TGCCAGCGGG 4 3800

GCGGCCTGCG CCGTGCGCTG GCGTCGCCAT TCGCGCATGC CGGCGCTGGC GTCGTTCATG 43860

TTGACGCGGG CGGCCTTACG CACTGCATCC ACGGTCGGGA AGTTCTCCCG GTCGCCTTGC 4 3920

TCGAACAGCT CGTCCGCAGC CGCAAAAATG CGGTCGCGCG TCTCTTTGTT CAGTTCCATG 4 3980TTGGCTCCGG TAATTGGTAA GAATAATAAT ACTCTTACCT ACCTTATCAG CGCAAGAGTT 44 040

TAGCTGAACA GTTCTCGACT TAACGGCAGG TTTTTTAGCG GCTGAAGGGC AGGCAAAAAA 44100

AGCCCCGCAC GGTCGGCGGG GGCAAAGGGT CAGCGGGAAG GGGATTAGCG GGCGTCGGGC 4 4160

TTCTTCATGC GTCGGGGCCG CGCTTCTTGG GATGGAGCAC GACGAAGCGC GCACGCGCAT 4 4 220

CGTCCTCGGC CCTATCGGCC CGCGTCGCGG TCAGGAACTT GTCGCGCGCT AGGTCCTCCC 4 4280

TGGTGGGCAC CAGGGGCATG AACTCGGCCT GCTCGATGTA GGTCCACTCC ATGACCGCAT 4 4 340

CGCAGTCGAG GCCGCGTTCC TTCACCGTCT CTTGCAGGTC GCGGTACGCC CGCTCGTTGA 44400

GCGGCTGGTA ACGGGCCAAT TGGTCGTAAA TGGCTGTCGG CCATGAGCGG CCTTTCCTGT 444 60

TGAGCCAGCA GCCGACGACG AAGCCGGCAA TGCAGGCCCC TGGCACAACC AGGCCGACGC 44520

CGGGGGCAGG GGATGGCAGC AGCTCGCCAA CCAGGAACCC CGCCGCGATG ATGCCGATGC 4 4 580

CGGTCAACCA GCCCTTGAAA CTATCCGGCC CCGAAACACC CCTGCGCATT GCCTGGATGC 4 4 640

TGCGCCGGAT AGCTTGCAAC ATCAGGAGCC GTTTCTTTTG TTCGTCAGTC ATGGTCCGCC 44700

CTCACCAGTT GTTCGTATCG GTGTCGGACG AACTGAAATC GCAAGAGCTG CCGGTATCGG 4 4 760

TCCAGCCGCT GTCCGTGTCG CTGCTGCCGA AGCACGGCGA GGGGTCCGCG AACGCCGCAG 4 4 820

ACGGCGTATC CGGCCGCAGC GCATCGCCCA GCATGGCCCC GGTCAGCGAG CCGCCGGCCA 4 4 880

GGTAGCCCAG CATGGTGCTG TTGGTCGCCC CGGCCACCAG GGCCGACGTG ACGAAATCGC 4 4 940

CGTCATTCCC TCTGGATTGT TCGCTGCTCG GCGGGGCAGT GCGCCGCGCC GGCGGCGTCG 4 5000

TGGATGGCTC GGGTTGGCTG GCCTGCGACG GCCGGCGAAA GGTGCGCAGC AGCTCGTTAT 4 5060

CGACCGGCTG CGGCGTCGGG GCCGCCGCCT TGCGCTGCGG TCGGTGTTCC TTCTTCGGCT 4 5120

CGCGCAGCTT GAACAGCATG ATCGCGGAAA CCAGCAGCAA CGCCGCGCCT ACGCCTCCCG 4 5180

CGATGTAGAA CAGCATCGGA TTCATTCTTC GGTCCTCCTT GTAGCGGAAC CGTTGTCTGT 4 524 0

GCGGCGCGGG TGGCCCGCGC CGCTGTCTTT GGGGATCAGC CCTCGATGAG CGCGACCAGT 4 5300

TTCACGTCGG CAAGGTTCGC CTCGAACTCC TGGCCGTCGT CCTCGTACTT CAACCAGGCA 4 5360

TAGCCTTCCG CCGGCGGCCG ACGGTTGAGG ATAAGGCGGG CAGGGCGCTC GTCGTGCTCG 4 5420

ACCTGGACGA TGGCCTTTTT CAGCTTGTCC GGGTCCGGCT CCTTCGCGCC CTTTTCCTTG 45480

GCGTCCTTAC CGTCCTGGTC GCCGTCCTCG CCGTCCTGGC CGTCGCCGGC CTCCGCGTCA 4 554 0

CGCTCGGCAT CAGTCTGGCC GTTGAAGGCA TCGACGGTGT TGGGATCGCG GCCCTTCTCG 4 5600

TCCAGGAACT CGCGCAGCAG CTTGACCGTG CCGCGCGTGA TTTCCTGGGT GTCGTCGTCA 4 5660

AGCCACGCCT CGACTTCCTC CGGGCGCTTC TTGAAGGCCG TCACCAGCTC GTTCACCACG 4 5720

GTCACGTCGC GCACGCGGCC GGTGTTGAAC GCATCGGCGA TCTTCTCCGG CAGGTCCAGC 4 5780

AGCGTGACGT GCTGGGTGAT GAACGCCGGC GACTTGCCGA TTTCCTTGGC GATATCGCCT 4 584 0

TTCTTCTTGC CCTTCGCCAG CTCGCGGCCA ATGAAGTCGG CAATTTCGCG CGGGGTCAGC 4 5900

TCGTTGCGTT GCAGGTTCTC GATAACCTGG TCGGCTTCGT TGTAGTCGTT GTCGATGAAC 4 5960

GCCGGGATGG ACTTCTTGCC GGCCCACTTC GAGCCACGGT AGCGGCGGGC GCCGTGATTG 4 6020

ATGATATAGC GGCCCGGCTG CTCCTGGTTC TCGCGCACCG AAATGGGTGA CTTCACCCCG 4 6080

CGCTCTTTGA TCGTGGCACC GATTTCCGCG ATGCTCTCCG GGGAAAAGCC GGGGTTGTCG 4 614 0

GCCGTCCGCG GCTGATGCGG ATCTTCGTCG ATCAGGTCCA GGTCCAGCTC GATAGGGCCG 4 6200

GAACCGCCCT GAGACGCCGC AGGAGCGTCC AGGAGGCTCG ACAGGTCGCC GATGCTATCC 4 6260

AACCCCAGGC CGGACGGCTG CGCCGCGCCT GCGGCTTCCT GAGCGGCCGC AGCGGTGTTT 4 6320

TTCTTGGTGG TCTTGGCTTG AGCCGCAGTC ATTGGGAAAT CTCCATCTTC GTGAACACGT 4 6380

AATCAGCCAG GGCGCGAACC TCTTTCGATG CCTTGCGCGC GGCCGTTTTC TTGATCTTCC 4 6440

AGACCGGCAC ACCGGATGCG AGGGCATCGG CGATGCTGCT GCGCAGGCCA ACGGTGGCCG 4 6500

GAATCATCAT CTTGGGGTAC GCGGCCAGCA GCTCGGCTTG GTGGCGCGCG TGGCGCGGAT 4 6560

TCCGCGCATC GACCTTGCTG GGCACCATGC CAAGGAATTG CAGCTTGGCG TTCTTCTGGC 4 6620

GCACGTTCGC AATGGTCGTG ACCATCTTCT TGATGCCCTG GATGCTGTAC GCCTCAAGCT 4 6680

CGATGGGGGA CAGCACATAG TCGGCCGCGA AGAGGGCGGC CGCCAGGCCG ACGCCAAGGG 4 6740

TCGGGGCCGT GTCGATCAGG CACACGTCGA AGCCTTGGTT CGCCAGGGCC TTGATGTTCG 4 6800

CCCCGAACAG CTCGCGGGCG TCGTCCAGCG ACAGCCGTTC GGCGTTCGCC AGTACCGGGT 4 68 60

TGGACTCGAT GAGGGCGAGG CGCGCGGCCT GGCCGTCGCC GGCTGCGGGT GCGGTTTCGG 4 6920

TCCAGCCGCC GGCAGGGACA GCGCCGAACA GCTTGCTTGC ATGCAGGCCG GTAGCAAAGT 4 6980

CCTTGAGCGT GTAGGACGCA TTGCCCTGGG GGTCCAGGTC GATCACGGCA ACCCGCAAGC 47 040

CGCGCTCGAA AAAGTCGAAG GCAAGATGCA CAAGGGTCGA AGTCTTGCCG ACGCCGCCTT 47100

TCTGGTTGGC CGTGACCAAA GTTTTCATCG TTTGGTTTCC TGTTTTTTCT TGGCGTCCGC 47160

TTCCCACTTC CGGACGATGT ACGCCTGATG TTCCGGCAGA ACCGCCGTTA CCCGCGCGTA 47220

CCCCTCGGGC AAGTTCTTGT CCTCGAACGC GGCCCACACG CGATGCACCG CTTGCGACAC 47280

TGCGCCCCTG GTCAGTCCCA GCGACGTTGC GAACGTCGCC TGTGGCTTCC CATCGACTAA 4 7340

GACGCCCCGC GCTATCTCGA TGGTCTGCTG CCCCACTTCC AGCCCCTGGA TCGCCTCCTG 47 400

GAACTGGCTT TCGGTAAGCC GTTTCTTCAT GGATAACACC CATAATTTGC TCCGCGCCTT 47 4 60

GGTTGAACAT AGCGGTGACA GCCGCCAGCA CATGAGAGAA GTTTAGCTAA ACATTTCTCG 4 7520CACGTCAACA CCTTTAGCCG CTAAAACTCG TCCTTGGCGT AACAAAACAA AAGCCCGGAA 47580

ACCGGGCTTT CGTCTCTTGC CGCTTATGGC TCTGCACCCG GCTCCATCAC CAACAGGTCG 4 7 640

CGCACGCGCT TCACTCGGTT GCGGATCGAC ACTGCCAGCC CAACAAAGCC GGTTGCCGCC 4 7 700

GCCGCCAGGA TCGCGCCGAT GATGCCGGCC ACACCGGCCA TCGCCCACCA GGTCGCCGCC 4 7760

TTCCGGTTCC ATTCCTGCTG GTACTGCTTC GCAATGCTGG ACCTCGGCTC ACCATAGGCT 47820

GACCGCTCGA TGGCGTATGC CGCTTCTCCC CTTGGCGTAA AACCCAGCGC CGCAGGCGGC 47880

ATTGCCATGC TGCCCGCCGC TTTCCCGACC ACGACGCGCG CACCAGGCTT GCGGTCCAGA 4 7 940

CCTTCGGCCA CGGCGAGCTG CGCAAGGACA TAATCAGCCG CCGACTTGGC TCCACGCGCC 4 8000

TCGATCAGCT CTTGCACTCG CGCGAAATCC TTGGCCTCCA CGGCCGCCAT GAATCGCGCA 48060

CGCGGCGAAG GCTCCGCAGG GCCGGCGTCG TGATCGCCGC CGAGAATGCC CTTCACCAAG 4 8120

TTCGACGACA CGAAAATCAT GCTGACGGCT ATCACCATCA TGCAGACGGA TCGCACGAAC 4 8180

CCGCTGAATT GAACACGAGC ACGGCACCCG CGACCACTAT GCCAAGAATG CCCAAGGTAA 4 8240

AAATTGCCGG CCCCGCCATG AAGTCCGTGA ATGCCCCGAC GGCCGAAGTG AAGGGCAGGC 4 8300

CGCCACCCAG GCCGCCGCCC TCACTGCCCG GCACCTGGTC GCTGAATGTC GATGCCAGCA 4 8360

CCTGCGGCAC GTCAATGCTT CCGGGCGTCG CGCTCGGGCT GATCGCCCAT CCCGTTACTG 4 8420

CCCCGATCCC GGCAATGGCA AGGACTGCCA GCGCTGCCAT TTTTGGGGTG AGGCCGTTCG 48480

CGGCCGAGGG GCGCAGCCCC TGGGGGGATG GGAGGCCCGC GTTAGCGGGC CGGGAGGGTT 48540

CGAGAAGGGG GGGCACCCCC CTTCGGCGTG CGCGGTCACG CGCACAGGGC GCAGCCCTGG 4 8600

TTAAAAACAA GGTTTATAAA TATTGGTTTA AAAGCAGGTT AAAAGACAGG TTAGCGGTGG 48660

CCGAAAAACG GGCGGAAACC CTTGCAAATG CTGGATTTTC TGCCTGTGGA CAGCCCCTCA 4 8720

AATGTCAATA GGTGCGCCCC TCATCTGTCA GCACTCTGCC CCTCAAGTGT CAAGGATCGC 48780

GCCCCTCATC TGTCAGTAGT CGCGCCCCTC AAGTGTCAAT ACCGCAGGGC ACTTATCCCC 4 8840

AGGCTTGTCC ACATCATCTG TGGGAAACTC GCGTAAAATC AGGCGTTTTC GCCGATTTGC 4 8 900

GAGGCTGGCC AGCTCCACGT CGCCGGCCGA AATCGAGCCT GCCCCTCATC TGTCAACGCC 4 8 960

GCGCCGGGTG AGTCGGCCCC TCAAGTGTCA ACGTCCGCCC CTCATCTGTC AGTGAGGGCC 4 9020

AAGTTTTCCG CGAGGTATCC ACAACGCCGG CGGCCGCGGT GTCTCGCACA CGGCTTCGAC 4 9080

GGCGTTTCTG GCGCGTTTGC AGGGCCATAG ACGGCCGCCA GCCCAGCGGC GAGGGCAACC 4 9140

AGCCCGGTGA GCGTCGGAAA GGCGCTGGAA GCCCCGTAGC GACGCGGAGA GGGGCGAGAC 4 9200

AAGCCAAGGG CGCAGGCTCG ATGCGCAGCA CGACATAGCC GGTTCTCGCA AGGACGAGAA 4 9260

TTTCCCTGCG GTGCCCCTCA AGTGTCAATG AAAGTTTCCA ACGCGAGCCA TTCGCGAGAG 4 9320

CCTTGAGTCC ACGCTAGATG AGAGCTTTGT TGTAGGTGGA CCAGTTGGTG ATTTTGAACT 4 9380

TTTGCTTTGC CACGGAACGG TCTGCGTTGT CGGGAAGATG CGTGATCTGA TCCTTCAACT 4 9440

CAGCAAAAGT TCGATTTATT CAACAAAGCC ACGTTGTGTC TCAAAATCTC TGATGTTACA 4 9500

TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA 4 9560

ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAAC 4 9597

<210> 94<211> 49579<212> DNA<213> VETOR

<4 00> 94

GTCTTGCTCG ACTCTAGAGC TCGTTCCTCG AGGCCTCGAG GCCTCGAGGA ACGGTACCTG 60

CGGGGAAGCT TACAATAATG TGTGTTGTTA AGTCTTGTTG CCTGTCATCG TCTGACTGAC 120

TTTCGTCATA AATCCCGGCC TCCGTAACCC AGCTTTGGGC AAGCTCACGG ATTTGATCCG 180

GCGGAACGGG AATATCGAGA TGCCGGGCTG AACGCTGCAG TTCCAGCTTT CCCTTTCGGG 24 0

ACAGGTACTC CAGCTGATTG ATTATCTGCT GAAGGGTCTT GGTTCCACCT CCTGGCACAA 300

TGCGAATGAT TACTTGAGCG CGATCGGGCA TCCAATTTTC TCCCGTCAGG TGCGTGGTCA 360

AGTGCTACAA GGCACCTTTC AGTAACGAGC GACCGTCGAT CCGTCGCCGG GATACGGACA 420

AAATGGAGCG CAGTAGTCCA TCGAGGGCGG CGAAAGCCTC GCCAAAAGCA ATACGTTCAT 480

CTCGCACAGC CTCCAGATCC GATCGAGGGT CTTCGGCGTA GGCAGATAGA AGCATGGATA 54 0

CATTGCTTGA GAGTATTCCG ATGGACTGAA GTATGGCTTC CATCTTTTCT CGTGTGTCTG 600

CATCTATTTC GAGAAAGCCC CCGATGCGGC GCACCGCAAC GCGAATTGCC ATACTATCCG 660

AAAGTCCCAG CAGGCGCGCT TGATAGGAAA AGGTTTCATA CTCGGCCGAT CGCAGACGGG 720

CACTCACGAC CTTGAACCCT TCAACTTTCA GGGATCGATG CTGGTTGATG GTAGTCTCAC 780

TCGACGTGGC TCTGGTGTGT TTTGACATAG CTTCCTCCAA AGAAAGCGGA AGGTCTGGAT 84 0

ACTCCAGCAC GAAATGTGCC CGGGTAGACG GATGGAAGTC TAGCCCTGCT CAATATGAAA 900

TCAACAGTAC ATTTACAGTC AATACTGAAT ATACTTGCTA CATTTGCAAT TGTCTTATAA 960

CGAATGTGAA ATAAAAATAG TGTAACAACG CTTTTACTCA TCGATAATCA CAAAAACATT 1020TATACGAACA AAAATACAAA TGCACTCCGG TTTCACAGGA TAGGCGGGAT CAGAATATGC 1080

AACTTTTGAC GTTTTGTTCT TTCAAAGGGG GTGCTGGCAA AACCACCGCA CTCATGGGCC 1140

TTTGCGCTGC TTTGGCAAAT GACGGTAAAC GAGTGGCCCT CTTTGATGCC GACGAAAACC 1200

GGCCTCTGAC GCGATGGAGA GAAAACGCCT TACAAAGCAG TACTGGGATC CTCGCTGTGA 1260

AGTCTATTCC GCCGACGAAA TGCCCCTTCT TGAAGCAGCC TATGAAAATG CCGAGCTCGA 1320

AGGATTTGAT TATGCGTTGG CCGATACGCG TGGCGGCTCG AGCGAGCTCA ACAACACAAT 1380

CATCGCTAGC TCAAACCTGC TTCTGATCCC CACCATGCTA ACGCCGCTCG ACATCGATGA 14 4 0

GGCACTATCT ACCTACCGCT ACGTCATCGA GCTGCTGTTG AGTGAAAATT TGGCAATTCC 1500

TACAGCTGTT TTGCGCCAAC GCGTCCCGGT CGGCCGATTG ACAACATCGC AACGCAGGAT 1560

GTCAGAGACG CTAGAGAGCC TTCCAGTTGT ACCGTCTCCC ATGCATGAAA GAGATGCATT 1620

TGCCGCGATG AAAGAACGCG GCATGTTGCA TCTTACATTA CTAAACACGG GAACTGATCC 1680

GACGATGCGC CTCATAGAGA GGAATCTTCG GATTGCGATG GAGGAAGTCG TGGTCATTTC 17 4 0

GAAACTGATC AGCAAAATCT TGGAGGCTTG AAGATGGCAA TTCGCAAGCC CGCATTGTCG 1800

GTCGGCGAAG CACGGCGGCT TGCTGGTGCT CGACCCGAGA TCCACCATCC CAACCCGACA 18 60

CTTGTTCCCC AGAAGCTGGA CCTCCAGCAC TTGCCTGAAA AAGCCGACGA GAAAGACCAG 1920

CAACGTGAGC CTCTCGTCGC CGATCACATT TACAGTCCCG ATCGACAACT TAAGCTAACT 1980

GTGGATGCCC TTAGTCCACC TCCGTCCCCG AAAAAGCTCC AGGTTTTTCT TTCAGCGCGA 204 0

CCGCCCGCGC CTCAAGTGTC GAAAACATAT GACAACCTCG TTCGGCAATA CAGTCCCTCG 2100

AAGTCGCTAC AAATGATTTT AAGGCGCGCG TTGGACGATT TCGAAAGCAT GCTGGCAGAT 2160

GGATCATTTC GCGTGGCCCC GAAAAGTTAT CCGATCCCTT CAACTACAGA AAAATCCGTT 2220

CTCGTTCAGA CCTCACGCAT GTTCCCGGTT GCGTTGCTCG AGGTCGCTCG AAGTCATTTT 2280

GATCCGTTGG GGTTGGAGAC CGCTCGAGCT TTCGGCCACA AGCTGGCTAC CGCCGCGCTC 234 0

GCGTCATTCT TTGCTGGAGA GAAGCCATCG AGCAATTGGT GAAGAGGGAC CTATCGGAAC 24 00

CCCTCACCAA ATATTGAGTG TAGGTTTGAG GCCGCTGGCC GCGTCCTCAG TCACCTTTTG 24 60

AGCCAGATAA TTAAGAGCCA AATGCAATTG GCTCAGGCTG CCATCGTCCC CCCGTGCGAA 2 520

ACCTGCACGT CCGCGTCAAA GAAATAACCG GCACCTCTTG CTGTTTTTAT CAGTTGAGGG 2 580

CTTGACGGAT CCGCCTCAAG TTTGCGGCGC AGCCGCAAAA TGAGAACATC TATACTCCTG 2 64 0

TCGTAAACCT CCTCGTCGCG TACTCGACTG GCAATGAGAA GTTGCTCGCG CGATAGAACG 2700

TCGCGGGGTT TCTCTAAAAA CGCGAGGAGA AGATTGAACT CACCTGCCGT AAGTTTCACC 2760

TCACCGCCAG CTTCGGACAT CAAGCGACGT TGCCTGAGAT TAAGTGTCCA GTCAGTAAAA 2820

CAAAAAGACC GTCGGTCTTT GGAGCGGACA ACGTTGGGGC GCACGCGCAA GGCAACCCGA 2880

ATGCGTGCAA GAAACTCTCT CGTACTAAAC GGCTTAGCGA TAAAATCACT TGCTCCTAGC 2 94 0

TCGAGTGCAA CAACTTTATC CGTCTCCTCA AGGCGGTCGC CACTGATAAT TATGATTGGA 3000

ATATCAGACT TTGCCGCCAG ATTTCGAACG ATCTCAAGCC CATCTTCACG ACCTAAATTT 3060

AGATCAACAA CCACGACATC GACCGTCGCG GAAGAGAGTA CTCTAGTGAA CTGGGTGCTG 3120

TCGGCTACCG CGGTCACTTT GAAGGCGTGG ATCGTAAGGT ATTCGATAAT AAGATGCCGC 3180

ATAGCGACAT CGTCATCGAT AAGAAGAACG TGTTTCAACG GCTCACCTTT CAATCTAAAA 324 0

TCTGAACCCT TGTTCACAGC GCTTGAGAAA TTTTCACGTG AAGGATGTAC AATCATCTCC 3300

AGCTAAATGG GCAGTTCGTC AGAATTGCGG CTGACCGCGG ATGACGAAAA TGCGAACCAA 3360

GTATTTCAAT TTTATGACAA AAGTTCTCAA TCGTTGTTAC AAGTGAAACG CTTCGAGGTT 34 20

ACAGCTACTA TTGATTAAGG AGATCGCCTA TGGTCTCGCC CCGGCGTCGT GCGTCCGCCG 34 80

CGAGCCAGAT CTCGCCTACT TCATAAACGT CCTCATAGGC ACGGAATGGA ATGATGACAT 354 0

CGATCGCCGT AGAGAGCATG TCAATCAGTG TGCGATCTTC CAAGCTAGCA CCTTGGGCGC 3600

TACTTTTGAC AAGGGAAAAC AGTTTCTTGA ATCCTTGGAT TGGATTCGCG CCGTGTATTG 3660

TTGAAATCGA TCCCGGATGT CCCGAGACGA CTTCACTCAG ATAAGCCCAT GCTGCATCGT 3720

CGCGCATCTC GCCAAGCAAT ATCCGGTCCG GCCGCATACG CAGACTTGCT TGGAGCAAGT 3780

GCTCGGCGCT CACAGCACCC AGCCCAGCAC CGTTCTTGGA GTAGAGTAGT CTAACATGAT 3840

TATCGTGTGG AATGACGAGT TCGAGCGTAT CTTCTATGGT GATTAGCCTT TCCTGGGGGG 3900

GGATGGCGCT GATCAAGGTC TTGCTCATTG TTGTCTTGCC GCTTCCGGTA GGGCCACATA 3960

GCAACATCGT CAGTCGGCTG ACGACGCATG CGTGCAGAAA CGCTTCCAAA TCCCCGTTGT 4 020

CAAAATGCTG AAGGATAGCT TCATCATCCT GATTTTGGCG TTTCCTTCGT GTCTGCCACT 4080

GGTTCCACCT CGAAGCATCA TAACGGGAGG AGACTTCTTT AAGACCAGAA ACACGCGAGC 4140

TTGGCCGTCG AATGGTCAAG CTGACGGTGC CCGAGGGAAC GGTCGGCGGC AGACAGATTT 4 200

GTAGTCGTTC ACCACCAGGA AGTTCAGTGG CGCAGAGGGG GTTACGTGGT CCGACATCCT 4260

GCTTTCTCAG CGCGCCCGCT AAAATAGCGA TATCTTCAAG ATCATCATAA GAGACGGGCA 4320

AAGGCATCTT GGTAAAAATG CCGGCTTGGC GCACAAATGC CTCTCCAGGT CGATTGATCG 4 380

CAATTTCTTC AGTCTTCGGG TCATCGAGCC ATTCCAAAAT CGGCTTCAGA AGAAAGCGTA 4 44 0

GTTGCGGATC CACTTCCATT TACAATGTAT CCTATCTCTA AGCGGAAATT TGAATTCATT 4 500

AAGAGCGGCG GTTCCTCCCC CGCGTGGCGC CGCCAGTCAG GCGGAGCTGG TAAACACCAA 4 560AGAAATCGAG GTCCCGTGCT ACGAAAATGG AAACGGTGTC ACCCTGATTC TTCTTCAGGG 4 620

TTGGCGGTAT GTTGATGGTT GCCTTAAGGG CTGTCTCAGT TGTCTGCTCA CCGTTATTTT 4 680

GAAAGCTGTT GAAGCTCATC CCGCCACCCG AGCTGCCGGC GTAGGTGCTA GCTGCCTGGA 4740

AGGCGCCTTG AACAACACTC AAGAGCATAG CTCCGCTAAA ACGCTGCCAG AAGTGGCTGT 4 800

CGACCGAGCC CGGCAATCCT GAGCGACCGA GTTCGTCCGC GCTTGGCGAT GTTAACGAGA 4 860

TCATCGCATG GTCAGGTGTC TCGGCGCGAT CCCACAACAC AAAAACGCGC CCATCTCCCT 4 920

GTTGCAAGCC ACGCTGTATT TCGCCAACAA CGGTGGTGCC ACGATCAAGA AGCACGATAT 4 980

TGTTCGTTGT TCCACGAATA TCCTGAGGCA AGACACACTT TACATAGCCT GCCAAATTTG 5040

TGTCGATTGC GGTTTGCAAG ATGCACGGAA TTATTGTCCC TTGCGTTACC ATAAAATCGG 5100

GGTGCGGCAA GAGCGTGGCG CTGCTGGGCT GCAGCTCGGT GGGTTTCATA CGTATCGACA 5160

AATCGTTCTC GCCGGACACT TCGCCATTCG GCAAGGAGTT GTCGTCACGC TTGCCTTCTT 5220

GTCTTCGGCC CGTGTCGCCC TGAATGGCGC GTTTGCTGAC CCCTTGATCG CCGCTGCTAT 5280

ATGCAAAAAT CGGTGTTTCT TCCGGCCGTG GCTCATGCCG CTCCGGTTCG CCCCTCGGCG 5340

GTAGAGGAGC AGCAGGCTGA ACAGCCTCTT GAACCGCTGG AGGATCCGGC GGCACCTCAA 5400

TCGGAGCTGG ATGAAATGGC TTGGTGTTTG TTGCGATCAA AGTTGACGGC GATGCGTTCT 54 60

CATTCACCTT CTTTTGGCGC CCACCTAGCC AAATGAGGCT TAATGATAAC GCGAGAACGA 5520

CACCTCCGAC GATCAATTTC TGAGACCCCG AAAGACGCCG GCGATGTTTG TCGGAGACCA 5580

GGGATCCAGA TGCATCAACC TCATGTGCCG CTTGCTGACT ATCGTTATTC ATCCCTTCGC 564 0

CCCCTTCAGG ACGCGTTTCA CATCGGGCCT CACCGTGCCC GTTTGCGGCC TTTGGCCAAC 5700

GGGATCGTAA GCGGTGTTCC AGATACATAG TACTGTGTGG CCATCCCTCA GACGCCAACC 57 60

TCGGGAAACC GAAGAAATCT CGACATCGCT CCCTTTAACT GAATAGTTGG CAACAGCTTC 5820

CTTGCCATCA GGATTGATGG TGTAGATGGA GGGTATGCGT ACATTGCCCG GAAAGTGGAA 5880

TACCGTCGTA AATCCATTGT CGAAGACTTC GAGTGGCAAC AGCGAACGAT CGCCTTGGGC 594 0

GACGTAGTGC CAATTACTGT CCGCCGCACC AAGGGCTGTG ACAGGCTGAT CCAATAAATT 6000

CTCAGCTTTC CGTTGATATT GTGCTTCCGC GTGTAGTCTG TCCACAACAG CCTTCTGTTG 6060

TGCCTCCCTT CGCCGAGCCG CCGCATCGTC GGCGGGGTAG GCGAATTGGA CGCTGTAATA 6120

GAGATCGGGC TGCTCTTTAT CGAGGTGGGA CAGAGTCTTG GAACTTATAC TGAAAACATA 6180

ACGGCGCATC CCGGAGTCGC TTGCGGTTAG CACGATTACT GGCTGAGGCG TGAGGACCTG 624 0

GCTTGCCTTG AAAAATAGAT AATTTCCCCG CGGTAGGGCT GCTAGATCTT TGCTATTTGA 6300

AACGGCAACC GCTGTCACCG TTTCGTTCGT GGCGAATGTT ACGACCAAAG TAGCTCCAAC 6360

CGCCGTCGAG AGGCGCACCA CTTGATCGGG ATTGTAAGCC AAATAACGCA TGCGCGGATC 6420

TAGCTTGCCC GCCATTGGAG TGTCTTCAGC CTCCGCACCA GTCGCAGCGG CAAATAAACA 6480

TGCTAAAATG AAAAGTGCTT TTCTGATCAT GGTTCGCTGT GGCCTACGTT TGAAACGGTA 654 0

TCTTCCGATG TCTGATAGGA GGTGACAACC AGACCTGCCG GGTTGGTTAG TCTCAATCTG 6600

CCGGGCAAGC TGGTCACCTT TTCGTAGCGA ACTGTCGCGG TCCACGTACT CACCACAGGC 6660

ATTTTGCCGT CAACGACGAG GGTCCTTTTA TAGCGAATTT GCTGCGTGCT TGGAGTTACA 6720

TCATTTGAAG CGATGTGCTC GACCTCCACC CTGCCGCGTT TGCCAAGAAT GACTTGAGGC 6780

GAACTGGGAT TGGGATAGTT GAAGAATTGC TGGTAATCCT GGCGCACTGT TGGGGCACTG 6840

AAGTTCGATA CCAGGTCGTA GGCGTACTGA GCGGTGTCGG CATCATAACT CTCGCGCAGG 6900

CGAACGTACT CCCACAATGA GGCGTTAACG ACGGCCTCCT CTTGAGTTGC AGGCAATCGC 6960

GAGACAGACA CCTCGCTGTC AACGGTGCCG TCCGGCCGTA TCCATAGATA TACGGGCACA 7020

AGCCTGCTCA ACGGCACCAT TGTGGCTATA GCGAACGCTT GAGCAACATT TCCCAAAATC 7 080

GCGATAGCTG CGACAGCTGC AATGAGTTTG GAGAGACGTC GCGCCGATTT CGCTCGCGCG 7140

GTTTGAAAGG CTTCTACTTC CTTATAGTGC TCGGCAAGGC TTTCGCGCGC CACTAGCATG 7200

GCATATTCAG GCCCCGTCAT AGCGTCCACC CGAATTGCCG AGCTGAAGAT CTGACGGAGT 7260

AGGCTGCCAT CGCCCCACAT TCAGCGGGAA GATCGGGCCT TTGCAGCTCG CTAATGTGTC 7320

GTTTGTCTGG CAGCCGCTCA AAGCGACAAC TAGGCACAGC AGGCAATACT TCATAGAATT 7380

CTCCATTGAG GCGAATTTTT GCGCGACCTA GCCTCGCTCA ACCTGAGCGA AGCGACGGTA 7440

CAAGCTGCTG GCAGATTGGG TTGCGCCGCT CCAGTAACTG CCTCCAATGT TGCCGGCGAT 7 500

CGCCGGCAAA GCGACAATGA GCGCATCCCC TGTCAGAAAA AACATATCGA GTTCGTAAAG 7560

ACCAATGATC TTGGCCGCGG TCGTACCGGC GAAGGTGATT ACACCAAGCA TAAGGGTGAG 7 620

CGCAGTCGCT TCGGTTAGGA TGACGATCGT TGCCACGAGG TTTAAGAGGA GAAGCAAGAG 7680

ACCGTAGGTG ATAAGTTGCC CGATCCACTT AGCTGCGATG TCCCGCGTGC GATCAAAAAT 7740

ATATCCGACG AGGATCAGAG GCCCGATCGC GAGAAGCACT TTCGTGAGAA TTCCAACGGC 7800

GTCGTAAACT CCGAAGGCAG ACCAGAGCGT GCCGTAAAGG ACCCACTGTG CCCCTTGGAA 7860

AGCAAGGATG TCCTGGTCGT TCATCGGACC GATTTCGGAT GCGATTTTCT GAAAAACGGC 7 920

CTGGGTCACG GCGAACATTG TATCCAACTG TGCCGGAACA GTCTGCAGAG GCAAGCCGGT 7 980

TACACTAAAC TGCTGAACAA AGTTTGGGAC CGTCTTTTCG AAGATGGAAA CCACATAGTC 8040

TTGGTAGTTA GCCTGCCCAA CAATTAGAGC AACAACGATG GTGACCGTGA TCACCCGAGT 8100

ίGATACCGCTA CGGGTATCGA CTTCGCCGCG TATGACTAAA ATACCCTGAA CAATAATCCA 8160

AAGAGTGACA CAGGCGATCA ATGGCGCACT CACCGCCTCC TGGATAGTCT CAAGCATCGA 8220

GTCCAAGCCT GTCGTGAAGG CTACATCGAA GATCGTATGA ATGGCCGTAA ACGGCGCCGG 8280

AATCGTGAAA TTCATCGATT GGACCTGAAC TTGACTGGTT TGTCGCATAA TGTTGGATAA 834 0

AATGAGCTCG CATTCGGCGA GGATGCGGGC GGATGAACAA ATCGCCCAGC CTTAGGGGAG 84 00

GGCACCAAAG ATGACAGCGG TCTTTTGATG CTCCTTGCGT TGAGCGGCCG CCTCTTCCGC 84 60

CTCGTGAAGG CCGGCCTGCG CGGTAGTCAT CGTTAATAGG CTTGTCGCCT GTACATTTTG 8520

AATCATTGCG TCATGGATCT GCTTGAGAAG CAAACCATTG GTCACGGTTG CCTGCATGAT 8580

ATTGCGAGAT CGGGAAAGCT GAGCAGACGT ATCAGCATTC GCCGTCAAGC GTTTGTCCAT 864 0

CGTTTCCAGA TTGTCAGCCG CAATGCCAGC GCTGTTTGCG GAACCGGTGA TCTGCGATCG 87 00

CAACAGGTCC GCTTCAGCAT CACTACCCAC GACTGCACGA TCTGTATCGC TGGTGATCGC 87 60

ACGTGCCGTG GTCGACATTG GCATTCGCGG CGAAAACATT TCATTGTCTA GGTCCTTCGT 8820

CGAAGGATAC TGATTTTTCT GGTTGAGCGA AGTCAGTAGT CCAGTAACGC CGTAGGCCGA 8880

CGTCAACATC GTAACCATCG CTATAGTCTG AGTGAGATTC TCCGCAGTCG CGAGCGCAGT 894 0

CGCGAGCGTC TCAGCCTCCG TTGCCGGGTC GCTAACAACA AACTGCGCCC GCGCGGGCTG 9000

AATATATAGA AAGCTGCAGG TCAAAACTGT TGCAATAAGT TGCGTCGTCT TCATCGTTTC 9060

CTACCTTATC AATCTTCTGC CTCGTGGTGA CGGGCCATGA ATTCGCTGAG CCAGCCAGAT 9120

GAGTTGCCTT CTTGTGCCTC GCGTAGTCGA GTTGCAAAGC GCACCGTGTT GGCACGCCCC 9180

GAAAGCACGG CGACATATTC ACGCATATCC CGCAGATCAA ATTCGCAGAT GACGCTTCCA 924 0

CTTTCTCGTT TAAGAAGAAA CTTACGGCTG CCGACCGTCA TGTCTTCACG GATCGCCTGA 9300

AATTCCTTTT CGGTACATTT CAGTCCATCG ACATAAGCCG ATCGATCTGC GGTTGGTGAT 9360

GGATAGAAAA TCTTCGTCAT ACATTGCGCA ACCAAGCTGG CTCCTAGCGG CGATTCCAGA 9420

ACATGCTCTG GTTGCTGCGT TGCCAGTATT AGCATCCCGT TGTTTTTTCG AACGGTCAGG 9480

AGGAATTTGT CGACGACAGT CGAAAATTTA GGGTTTAACA AATAGGCGCG AAACTCATCG 9540

CAGCTCATCA CAAAACGGCG GCCGTCGATC ATGGCTCCAA TCCGATGCAG GAGATATGCT 9600

GCAGCGGGAG CGCATACTTC CTCGTATTCG AGAAGATGCG TCATGTCGAA GCCGGTAATC 9660

GACGGATCTA ACTTTACTTC GTCAACTTCG CCGTCAAATG CCCAGCCAAG CGCATGGCCC 9720

CGGCACCAGC GTTGGAGCCG CGCTCCTGCG CCTTCGGCGG GCCCATGCAA CAAAAATTCA 9780

CGTAACCCCG CGATTGAACG CATTTGTGGA TCAAACGAGA GCTGACGATG GATACCACGG 9840

ACCAGACGGC GGTTCTCTTC CGGAGAAATC CCACCCCGAC CATCACTCTC GATGAGAGCC 9900

ACGATCCATT CGCGCAGAAA ATCGTGTGAG GCTGCTGTGT TTTCTAGGCC ACGCAACGGC 9960

GCCAACCCGC TGGGTGTGCC TCTGTGAAGT GCCTVAATATG TTCCTCCTGT GGCGCGAACC 10020

AGCAATTCGC CACCCCGGTC CTTGTCAAAG AACACGACCG TACCTGCACG GTCGACCATG 10080

CTCTGTTCGA GCATGGCTAG AACAAACATC ATGAGCGTCG TCTTACCCCT CCCGATAGGC 1014 0

CCGAATATTG CCGTCATGCC AACATCGTGC TCATGCGGGA TATAGTCGAA AGGCGTTCCG 10200

CCATTGGTAC GAAATCGGGC AATCGCGTTG CCCCAGTGGC CTGAGCTGGC GCCCTCTGGA 10260

AAGTTTTCGA AAGAGACAAA CCCTGCGAAA TTGCGTGAAG TGATTGCGCC AGGGCGTGTG 10320

CGCCACTTAA AATTCCCCGG CAATTGGGAC CAATAGGCCG CTTCCATACC AATACCTTCT 10380

TGGACAACCA CGGCACCTGC ATCCGCCATT CGTGTCCGAG CCCGCGCGCC CCTGTCCCCA 104 40

AGACTATTGA GATCGTCTGC ATAGACGCAA AGGCTCAAAT GATGTGAGCC CATAACGAAT 10500

TCGTTGCTCG CAAGTGCGTC CTCAGCCTCG GATAATTTGC CGATTTGAGT CACGGCTTTA 10560

TCGCCGGAAC TCAGCATCTG GCTCGATTTG AGGCTAAGTT TCGCGTGCGC TTGCGGGCGA 10620

GTCAGGAACG AAAAACTCTG CGTGAGAACA AGTGGAAAAT CGAGGGATAG CAGCGCGTTG 10680

AGCATGCCCG GCCGTGTTTT TGCAGGGTAT TCGCGAAACG AATAGATGGA TCCAACGTAA 10740

CTGTCTTTTG GCGTTCTGAT CTCGAGTCCT CGCTTGCCGC AAATGACTCT GTCGGTATAA 10800

ATCGAAGCGC CGAGTGAGCC GCTGACGACC GGAACCGGTG TGAACCGACC AGTCATGATC 10860

AACCGTAGCG CTTCGCCAAT TTCGGTGAAG AGCACACCCT GCTTCTCGCG GATGCCAAGA 10920

CGATGCAGGC CATACGCTTT AAGAGAGCCA GCGACAACAT GCCAAAGATC TTCCATGTTC 10980

CTGATCTGGC CCGTGAGATC GTTTTCCCTT TTTCCGCTTA GCTTGGTGAA CCTCCTCTTT 11040

ACCTTCCCTA AAGCCGCCTG TGGGTAGACA ATCAACGTAA GGAAGTGTTC ATTGCGGAGG 11100

AGTTGGCCGG AGAGCACGCG CTGTTCAAAA GCTTCGTTCA GGCTAGCGGC GAAAACACTA 11160

CGGAAGTGTC GCGGCGCCGA TGATGGCACG TCGGCATGAC GTACGAGGTG AGCATATATT 11220

GACACATGAT CATCAGCGAT ATTGCGCAAC AGCGTGTTGA ACGCACGACA ACGCGCATTG 11280

CGCATTTCAG TTTCCTCAAG CTCGAATGCA ACGCCATCAA TTCTCGCAAT GGTCATGATC 11340

GATCCGTCTT CAAGAAGGAC GATATGGTCG CTGAGGTGGC CAATATAAGG GAGATAGATC 11400

TCACCGGATC TTTCGGTCGT TCCACTCGCG CCGAGCATCA CACCATTCCT CTCCCTCGTG 114 60

GGGGAACCCT AATTGGATTT GGGCTAACAG TAGCGCCCCC CCAAACTGCA CTATCAATGC 11520

TTCTTCCCGC GGTCCGCAAA AATAGCAGGA CGACGCTCGC CGCATTGTAG TCTCGCTCCA 11580

CGATGAGCCG GGCTGCAAAC CATAACGGCA CGAGAACGAC TTCGTAGAGC GGGTTCTGAA 11640CGATAACGAT GACAAAGCCG GCGAACATCA TGAATAACCC TGCCAATGTC AGTGGCACCC 11700

CAAGAAACAA TGCGGGCCGT GTGGCTGCGA GGTAAAGGGT CGATTCTTCC AAACGATCAG 11760

CCATCAACTA CCGCCAGTGA GCGTTTGGCC GAGGAAGCTC GCCCCAAACA TGATAACAAT 11820

GCCGCCGACG ACGCCGGCAA CCAGCCCAAG CGAAGCCCGC CCGAACATCC AGGAGATCCC 11880

GATAGCGACA ATGCCGAGAA CAGCGAGTGA CTGGCCGAAC GGACCAAGGA TAAACGTGCA 11940

TATATTGTTA ACCATTGTGG CGGGGTCAGT GCCGCCACCC GCAGATTGCG CTGCGGCGGG 12000

TCCGGATGAG GAAATGCTCC ATGCAATTGC ACCGCACAAG CTTGGGGCGC AGCTCGATAT 12060

CACGCGCATC ATCGCATTCG AGAGCGAGAG GCGATTTAGA TGTAAACGGT ATCTCTCAAA 12120

GCATCGCATC AATGCGCACC TCCTTAGTAT AAGTCGAATA AGACTTGATT GTCGTCTGCG 12180

GATTTGCCGT TGTCCTGGTG TGGCGGTGGC GGAGCGATTA AACCGCCAGC GCCATCCTCC 12240

TGCGAGCGGC GCTGATATGA CCCCCAAACA TCCCACGTCT CTTCGGATTT TAGCGCCTCG 12300

TGATCGTCTT TTGGAGGCTC GATTAACGCG GGCACCAGCG ATTGAGCAGC TGTTTCAACT 12360

TTTCGCACGT AGCCGTTTGC AAAACCGCCG ATGAAATTAC CGGTGTTGTA AGCGGAGATC 12420

GCCCGACGAA GCGCAAATTG CTTCTCGTCA ATCGTTTCGC CGCCTGCATA ACGACTTTTC 12480

AGCATGTTTG CAGCGGCAGA TAATGATGTG CACGCCTGGA GCGCACCGTC AGGTGTCAGA 12540

CCGAGCATAG AAAAATTTCG AGAGTTTATT TGCATGAGGC CAACATCCAG CGAATGCCGT 12600

GCATCGAGAC GGTGCCTGAC GACTTGGGTT GCTTGGCTGT GATCTTGCCA GTGAAGCGTT 12660

TCGCCGGTCG TGTTGTCATG AATCGCTAAA GGATCAAAGC GACTCTCCAC CTTAGCTATC 12720

GCCGCAAGCG TAGATGTCGC AACTGATGGG GCACACTTGC GAGCAACATG GTCAAACTCA 12780

GCAGATGAGA GTGGCGTGGC AAGGCTCGAC GAACAGAAGG AGACCATCAA GGCAAGAGAA 1284 0

AGCGACCCCG ATCTCTTAAG CATACCTTAT CTCCTTAGCT CGCAACTAAC ACCGCCTCTC 12900

CCGTTGGAAG AAGTGCGTTG TTTTATGTTG AAGATTATCG GGAGGGTCGG TTACTCGAAA 12960

ATTTTCAATT GCTTCTTTAT GATTTCAATT GAAGCGAGAA ACCTCGCCCG GCGTCTTGGA 13020

ACGCAACATG GACCGAGAAC CGCGCATCCA TGACTAAGCA ACCGGATCGA CCTATTCAGG 13080

CCGCAGTTGG TCAGGTCAGG CTCAGAACGA AAATGCTCGG CGAGGTTACG CTGTCTGTAA 1314 0

ACCCATTCGA TGAACGGGAA GCTTCCTTCC GATTGCTCTT GGCAGGAATA TTGGCCCATG 13200

CCTGCTTGCG CTTTGCAAAT GCTCTTATCG CGTTGGTATC ATATGCCTTG TCCGCCAGCA 13260

GAAACGCACT CTAAGCGATT ATTTGTAAAA ATGTTTCGGT CATGCGGCGG TCATGGGCTT 13320

GACCCGCTGT CAGCGCAAGA CGGATCGGTC AACCGTCGGC ATCGACAACA GCGTGAATCT 13380

TGGTGGTCAA ACCGCCACGG GAACGTCCCA TACAGCCATC GTCTTGATCC CGCTGTTTCC 13440

CGTCGCCGCA TGTTGGTGGA CGCGGACACA GGAACTGTCA ATCATGACGA CATTCTATCG 13500

AAAGCCTTGG AAATCACACT CAGAATATGA TCCCAGACGT CTGCCTCACG CCATCGTACA 13560

AAGCGATTGT AGCAGGTTGT ACAGGAACCG TATCGATCAG GAACGTCTGC CCAGGGCGGG 13620

CCCGTCCGGA AGCGCCACAA GATGACATTG ATCACCCGCG TCAACGCGCG GCACGCGACG 13680

CGGCTTATTT GGGAACAAAG GACTGAACAA CAGTCCATTC GAAATCGGTG ACATCAAAGC 13740

GGGGACGGGT TATCAGTGGC CTCCAAGTCA AGCCTCAATG AATCAAAATC AGACCGATTT 13800

GCAAACCTGA TTTATGAGTG TGCGGCCTAA ATGATGAAAT CGTCCTTCTA GATCGCCTCC 13860

GTGGTGTAGC AACACCTCGC AGTATCGCCG TGCTGACCTT GGCCAGGGAA TTGACTGGCA 13920

AGGGTGCTTT CACATGACCG CTCTTTTGGC CGCGATAGAT GATTTCGTTG CTGCTTTGGG 13980

CACGTAGAAG GAGAGAAGTC ATATCGGAGA AATTCCTCCT GGCGCGAGAG CCTGCTCTAT 14 04 0

CGCGACGGCA TCCCACTGTC GGGAACAGAC CGGATCATTC ACGAGGCGAA AGTCGTCAAC 14100

ACATGCGTTA TAGGCATCTT CCCTTGAAGG ATGATCTTGT TGCTGCCAAT CTGGAGGTGC 14160

GGCAGCCGCA GGCAGATGCG ATCTCAGCGC AACTTGCGGC AAAACATCTC ACTCACCTGA 14220

AAACCACTAG CGAGTCTCGC GATCAGACGA AGGCCTTTTA CTTAACGACA CAATATCCGA 14280

TGTCTGCATC ACAGGCGTCG CTATCCCAGT CAATACTAAA GCGGTGCAGG AACTAAAGAT 14 34 0

TACTGATGAC TTAGGCGTGC CACGAGGCCT GAGACGACGC GCGTAGACAG TTTTTTGAAA 14 4 00

TCATTATCAA AGTGATGGCC TCCGCTGAAG CCTATCACCT CTGCGCCGGT CTGTCGGAGA 14 4 60

GATGGGCAAG CATTATTACG GTCTTCGCGC CCGTACATGC ATTGGACGAT TGCAGGGTCA 14 520

ATGGATCTGA GATCATCCAG AGGATTGCCG CCCTTACCTT CCGTTTCGAG TTGGAGCCAG 14 580

CCCCTAAATG AGACGACATA GTCGACTTGA TGTGACAATG CCAAGAGAGA GATTTGCTTA 14 640

ACCCGATTTT TTTGCTCAAG CGTAAGCCTA TTGAAGCTTG CCGGCATGAC GTCCGCGCCG 14700

AAAGAATATC CTACAAGTAA AACATTCTGC ACACCGAAAT GCTTGGTGTA GACATCGATT 14 7 60

ATGTGACCAA GATCCTTAGC AGTTTCGCTT GGGGACCGCT CCGACCAGAA ATACCGAAGT 14820

GAACTGACGC CAATGACAGG AATCCCTTCC GTCTGCAGAT AGGTACCATC GATAGATCTG 14 880

CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 14 940

GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 15000

GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA 15060

TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 15120

GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG 15180CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 15240

GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 15300

GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 15360

CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 15420

GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 154 80

ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 1554 0

CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 15600

GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 15660

TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 15720

AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 15780

ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 1584 0

GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 15900

AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 15960

GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 16020

AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 16080

ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 1614 0

GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 16200

ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 16260

CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 16320

CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 16380

AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGGGG GGGGGGGGGG 16440

GGGTTCCATT GTTCATTCCA CGGACAAAAA CAGAGAAAGG AAACGACAGA GGCCAAAAAG 16500

CTCGCTTTCA GCACCTGTCG TTTCCTTTCT TTTCAGAGGG TATTTTAAAT AAAAACATTA 16560

AGTTATGACG AAGAAGAACG GAAACGCCTT AAACCGGAAA ATTTTCATAA ATAGCGAAAA 16620

CCCGCGAGGT CCCTGTCGGA TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT 16680

ACATATCACA ACGTGCGTGG AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG 16740

TATCGTATTA ATTGATCTGC ATCAACTTAA CGTAAAAACA ACTTCAGACA ATACAAATCA 16800

GCGACACTGA ATACGGGGCA ACCTCATGTC CCCCCCCCCC CCCCCCCTGC AGGCATCGTG 168 60

GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 16920

GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 16980

GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 17 040

CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 17100

TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT 17160

ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 17220

AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 17280

AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 1734 0

CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 17 4 00

CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 174 60

GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 17520

CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 17580

AGGCCCTTTC GTCTTCAAGA ATTGGTCGAC GATCTTGCTG CGTTCGGATA TTTTCGTGGA 17640

GTTCCCGCCA CAGACCCGGA TTGAAGGCGA GATCCAGCAA CTCGCGCCAG ATCATCCTGT 177 00

GACGGAACTT TGGCGCGTGA TGACTGGCCA GGACGTCGGC CGAAAGAGCG ACAAGCAGAT 17760

CACGCTTTTC GACAGCGTCG GATTTGCGAT CGAGGATTTT TCGGCGCTGC GCTACGTCCG 17 820

CGACCGCGTT GAGGGATCAA GCCACAGCAG CCCACTCGAC CTTCTAGCCG ACCCAGACGA 17880

GCCAAGGGAT CTTTTTGGAA TGCTGCTCCG TCGTCAGGCT TTCCGACGTT TGGGTGGTTG 17940

AACAGAAGTC ATTATCGTAC GGAATGCCAA GCACTCCCGA GGGGAACCCT GTGGTTGGCA 18000

TGCACATACA AATGGACGAA CGGATAAACC TTTTCACGCC CTTTTAAATA TCCGTTATTC 18060

TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT 18120

TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT 18180

ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA 18240

CGTTGAAGGA GCCACTCAGC AAGCTGGTAC GATTGTAATA CGACTCACTA TAGGGCGAAT 18300

TGAGCGCTGT TTAAACGCTC TTCAACTGGA AGAGCGGTTA CCCGGACCGA AGCTTGCATG 18360

CCTGCAGTGC AGCGTGACCC GGTCGTGCCC CTCTCTAGAG ATAATGAGCA TTGCATGTCT 18420

AAGTTATAAA AAATTACCAC ATATTTTTTT TGTCACACTT GTTTGAAGTG CAGTTTATCT 184 80

ATCTTTATAC ATATATTTAA ACTTTACTCT ACGAATAATA TAATCTATAG TACTACAATA 1854 0

ATATCAGTGT TTTAGAGAAT CATATAAATG AACAGTTAGA CATGGTCTAA AGGACAATTG 18 600

AGTATTTTGA CAACAGGACT CTACAGTTTT ATCTTTTTAG TGTGCATGTG TTCTCCTTTT 18660

TTTTTGCAAA TAGCTTCACC TATATAATAC TTCATCCATT TTATTAGTAC ATCCATTTAG 18720GGTTTAGGGT TAATGGTTTT TATAGACTAA TTTTTTTAGT ACATCTATTT TATTCTATTT 187 80

TAGCCTCTAA ATTAAGAAAA CTAAAACTCT ATTTTAGTTT TTTTATTTAA TAATTTAGAT 1884 0

ATAAAATAGA ATAAAATAAA GTGACTAAAA ATTAAACAAA TACCCTTTAA GAAATTAAAA 18 900

AAACTAAGGA AACATTTTTC TTGTTTCGAG TAGATAATGC CAGCCTGTTA AACGCCGTCG 18960

ACGAGTCTAA CGGACACCAA CCAGCGAACC AGCAGCGTCG CGTCGGGCCA AGCGAAGCAG 19020

ACGGCACGGC ATCTCTGTCG CTGCCTCTGG ACCCCTCTCG AGAGTTCCGC TCCACCGTTG 19080

GACTTGCTCC GCTGTCGGCA TCCAGAAATT GCGTGGCGGA GCGGCAGACG TGAGCCGGCA 19140

CGGCAGGCGG CCTCCTCCTC CTCTCACGGC ACGGCAGCTA CGGGGGATTC CTTTCCCACC 19200

GCTCCTTCGC TTTCCCTTCC TCGCCCGCCG TAATAAATAG ACACCCCCTC CACACCCTCT 19260

TTCCCCAACC TCGTGTTGTT CGGAGCGCAC ACACACACAA CCAGATCTCC CCCAAATCCA 19320

CCCGTCGGCA CCTCCGCTTC AAGGTACGCC GCTCGTCCTC CCCCCCCCCC CCTCTCTACC 19380

TTCTCTAGAT CGGCGTTCCG GTCCATGGTT AGGGCCCGGT AGTTCTACTT CTGTTCATGT 19440

TTGTGTTAGA TCCGTGTTTG TGTTAGATCC GTGCTGCTAG CGTTCGTACA CGGATGCGAC 19500

CTGTACGTCA GACACGTTCT GATTGCTAAC TTGCCAGTGT TTCTCTTTGG GGAATCCTGG 19560

GATGGCTCTA GCCGTTCCGC AGACGGGATC GATTTCATGA TTTTTTTTGT TTCGTTGCAT 19620

AGGGTTTGGT TTGCCCTTTT CCTTTATTTC AATATATGCC GTGCACTTGT TTGTCGGGTC 19680

ATCTTTTCAT GCTTTTTTTT GTCTTGGTTG TGATGATGTG GTCTGGTTGG GCGGTCGTTC 19740

TAGATCGGAG TAGAATTCTG TTTCAAACTA CCTGGTGGAT TTATTAATTT TGGATCTGTA 19800

TGTGTGTGCC ATACATATTC ATAGTTACGA ATTGAAGATG ATGGATGGAA ATATCGATCT 19860

AGGATAGGTA TACATGTTGA TGCGGGTTTT ACTGATGCAT ATACAGAGAT GCTTTTTGTT 19920

CGCTTGGTTG TGATGATGTG GTGTGGTTGG GCGGTCGTTC ATTCGTTCTA GATCGGAGTA 19980

GAATACTGTT TCAAACTACC TGGTGTATTT ATTAATTTTG GAACTGTATG TGTGTGTCAT 20040

ACATCTTCAT AGTTACGAGT TTAAGATGGA TGGAAATATC GATCTAGGAT AGGTATACAT 20100

GTTGATGTGG GTTTTACTGA TGCATATACA TGATGGCATA TGCAGCATCT ATTCATATGC 20160

TCTAACCTTG AGTACCTATC TATTATAATA AACAAGTATG TTTTATAATT ATTTTGATCT 20220

TGATATACTT GGATGATGGC ATATGCAGCA GCTATATGTG GATTTTTTTA GCCCTGCCTT 20280

CATACGCTAT TTATTTGCTT GGTACTGTTT CTTTTGTCGA TGCTCACCCT GTTGTTTGGT 20340

GTTACTTCTG CAGGTCGACT CTAGAGGATC TACAAGTTTG TACAAAAAAG CAGGCTCCGC 204 00

GGCCGCCCCC TTCACCATGA CGATGGCTCG TCCTGGGGCG GCTTTGCCGC TGCTGCTGGT 204 60

CGTGGTCGGC GCTTGCTGCG CGCGCCTGGC GGCGGCAGTG CACCTCTCCG CGCTCGGCAG 20520

GACACTCATC GTCGAGGCGT CGCCGAAGGC CGGACAAGTC CTGCACGCCG GCGAGGACAC 20580

GATAACCGTG ACATGGCACC TCAACGCGTC GGCGTCCAGC GTCGGGTACA AGGCGCTGGA 20640

GGTGACCCTC TGCTACGCGC CGGCGAGCCA GGAGGACCGC GGGTGGCGCA AGGCCAACGA 20700

CGACTTGAGC AAGGACAAGG CGTGCCAGTT CAGGATCGCC CGGCATGCAT ACGCCGGCGG 207 60

CCAGGGGACG CTCCGGTACA GGGTCGCCCG CGACGTCCCC ACCGCGTCCT ACCACGTGCG 20820

CGCCTACGCG CTGGACGCGT CCGGGGCGCC GGTGGGCTAC GGCCAGACCG CGCCCGCCTA 20880

CTACTTCCAC GTCGCGGGCG TCTCGGGCGT CCACGCGTCC CTCCGGGTCG CCGCCGCCGT 20940

GCTCTCCGCG TTCTCCATCG CCGCGCTCGC CTTCTTTGTC GTCGTCGAGA AGAGGAGGAA 21000

GGACGAGTAG AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TGTTAACCTA 21060

GACTTGTCCA TCTTCTGGAT TGGCCAACTT AATTAATGTA TGAAATAAAA GGATGCACAC 21120

ATAGTGACAT GCTAATCACT ATAATGTGGG CATCAAAGTT GTGTGTTATG TGTAATTACT 21180

AGTTATCTGA ATAAAAGAGA AAGAGATCAT CCATATTTCT TATCCTAAAT GAATGTCACG 21240

TGTCTTTATA ATTCTTTGAT GAACCAGATG CATTTCATTA ACCAAATCCA TATACATATA 21300

AATATTAATC ATATATAATT AATATCAATT GGGTTAGCAA AACAAATCTA GTCTAGGTGT 21360

GTTTTGCGAA TTGCGGCCGC CACCGCGGTG GAGCTCGAAT TCCGGTCCGG GTCACCTTTG 21420

TCCACCAAGA TGGAACTGCG GCCGCTCATT AATTAAGTCA GGCGCGCCTC TAGTTGAAGA 21480

CACGTTCATG TCTTCATCGT AAGAAGACAC TCAGTAGTCT TCGGCCAGAA TGGCCATCTG 2154 0

GATTCAGCAG GCCTAGAAGG CCATTTAAAT CCTGAGGATC TGGTCTTCCT AAGGACCCGG 21600

GCGGTCCGAT TAAACTTTAA TTCGGACCGA AGCTTGCATG CCTGCAGTGC AGCGTGACCC 21660

GGTCGTGCCC CTCTCTAGAG ATAATGAGCA TTGCATGTCT AAGTTATAAA AAATTACCAC 21720

ATATTTTTTT TGTCACACTT GTTTGAAGTG CAGTTTATCT ATCTTTATAC ATATATTTAA 21780

ACTTTACTCT ACGAATAATA TAATCTATAG TACTACAATA ATATCAGTGT TTTAGAGAAT 21840

CATATAAATG AACAGTTAGA CATGGTCTAA AGGACAATTG AGTATTTTGA CAACAGGACT 21900

CTACAGTTTT ATCTTTTTAG TGTGCATGTG TTCTCCTTTT TTTTTGCAAA TAGCTTCACC 21960

TATATAATAC TTCATCCATT TTATTAGTAC ATCCATTTAG GGTTTAGGGT TAATGGTTTT 22020

TATAGACTAA TTTTTTTAGT ACATCTATTT TATTCTATTT TAGCCTCTAA ATTAAGAAAA 22080

CTAAAACTCT ATTTTAGTTT TTTTATTTAA TAATTTAGAT ATAAAATAGA ATAAAATAAA 2214 0

GTGACTAAAA ATTAAACAAA TACCCTTTAA GAAATTAAAA AAACTAAGGA AACATTTTTC 22200

TTGTTTCGAG TAGATAATGC CAGCCTGTTA AACGCCGTCG ACGAGTCTAA CGGACACCAA 22260CCAGCGAACC AGCAGCGTCG CGTCGGGCCA AGCGAAGCAG ACGGCACGGC ATCTCTGTCG 22320

CTGCCTCTGG ACCCCTCTCG AGAGTTCCGC TCCACCGTTG GACTTGCTCC GCTGTCGGCA 22380

TCCAGAAATT GCGTGGCGGA GCGGCAGACG TGAGCCGGCA CGGCAGGCGG CCTCCTCCTC 224 4 0

CTCTCACGGC ACCGGCAGCT ACGGGGGATT CCTTTCCCAC CGCTCCTTCG CTTTCCCTTC 22500

CTCGCCCGCC GTAATAAATA GACACCCCCT CCACACCCTC TTTCCCCAAC CTCGTGTTGT 22560

TCGGAGCGCA CACACACACA ACCAGATCTC CCCCAAATCC ACCCGTCGGC ACCTCCGCTT 22620

CAAGGTACGC CGCTCGTCCT CCCCCCCCCC CCTCTCTACC TTCTCTAGAT CGGCGTTCCG 22 680

GTCCATGCAT GGTTAGGGCC CGGTAGTTCT ACTTCTGTTC ATGTTTGTGT TAGATCCGTG 22740

TTTGTGTTAG ATCCGTGCTG CTAGCGTTCG TACACGGATG CGACCTGTAC GTCAGACACG 22800

TTCTGATTGC TAACTTGCCA GTGTTTCTCT TTGGGGAATC CTGGGATGGC TCTAGCCGTT 228 60

CCGCAGACGG GATCGATTTC ATGATTTTTT TTGTTTCGTT GCATAGGGTT TGGTTTGCCC 22920

TTTTCCTTTA TTTCAATATA TGCCGTGCAC TTGTTTGTCG GGTCATCTTT TCATGCTTTT 22980

TTTTGTCTTG GTTGTGATGA TGTGGTCTGG TTGGGCGGTC GTTCTAGATC GGAGTAGAAT 23040

TCTGTTTCAA ACTACCTGGT GGATTTATTA ATTTTGGATC TGTATGTGTG TGCCATACAT 23100

ATTCATAGTT ACGAATTGAA GATGATGGAT GGAAATATCG ATCTAGGATA GGTATACATG 23160

TTGATGCGGG TTTTACTGAT GCATATACAG AGATGCTTTT TGTTCGCTTG GTTGTGATGA 23220

TGTGGTGTGG TTGGGCGGTC GTTCATTCGT TCTAGATCGG AGTAGAATAC TGTTTCAAAC 23280

TACCTGGTGT ATTTATTAAT TTTGGAACTG TATGTGTGTG TCATACATCT TCATAGTTAC 23340

GAGTTTAAGA TGGATGGAAA TATCGATCTA GGATAGGTAT ACATGTTGAT GTGGGTTTTA 23400

CTGATGCATA TACATGATGG CATATGCAGC ATCTATTCAT ATGCTCTAAC CTTGAGTACC 234 60

TATCTATTAT AATAAACAAG TATGTTTTAT AATTATTTTG ATCTTGATAT ACTTGGATGA 23520

TGGCATATGC AGCAGCTATA TGTGGATTTT TTTAGCCCTG CCTTCATACG CTATTTATTT 23580

GCTTGGTACT GTTTCTTTTG TCGATGCTCA CCCTGTTGTT TGGTGTTACT TCTGCAGGTC 2364 0

GACTTTAACT TAGCCTAGGA TCCACACGAC ACCATGTCCC CCGAGCGCCG CCCCGTCGAG 23700

ATCCGCCCGG CCACCGCCGC CGACATGGCC GCCGTGTGCG ACATCGTGAA CCACTACATC 237 60

GAGACCTCCA CCGTGAACTT CCGCACCGAG CCGCAGACCC CGCAGGAGTG GATCGACGAC 23820

CTGGAGCGCC TCCAGGACCG CTACCCGTGG CTCGTGGCCG AGGTGGAGGG CGTGGTGGCC 23880

GGCATCGCCT ACGCCGGCCC GTGGAAGGCC CGCAACGCCT ACGACTGGAC CGTGGAGTCC 23940

ACCGTGTACG TGTCCCACCG CCACCAGCGC CTCGGCCTCG GCTCCACCCT CTACACCCAC 24000

CTCCTCAAGA GCATGGAGGC CCAGGGCTTC AAGTCCGTGG TGGCCGTGAT CGGCCTCCCG 24 060

AACGACCCGT CCGTGCGCCT CCACGAGGCC CTCGGCTACA CCGCCCGCGG CACCCTCCGC 24120

GCCGCCGGCT ACAAGCACGG CGGCTGGCAC GACGTCGGCT TCTGGCAGCG CGACTTCGAG 24180

CTGCCGGCCC CGCCGCGCCC GGTGCGCCCG GTGACGCAGA TCTGAGTCGA AACCTAGACT 24 240

TGTCCATCTT CTGGATTGGC CAACTTAATT AATGTATGAA ATAAAAGGAT GCACACATAG 24300

TGACATGCTA ATCACTATAA TGTGGGCATC AAAGTTGTGT GTTATGTGTA ATTACTAGTT 24 360

ATCTGAATAA AAGAGAAAGA GATCATCCAT ATTTCTTATC CTAAATGAAT GTCACGTGTC 24 420

TTTATAATTC TTTGATGAAC CAGATGCATT TCATTAACCA AATCCATATA CATATAAATA 24 480

TTAATCATAT ATAATTAATA TCAATTGGGT TAGCAAAACA AATCTAGTCT AGGTGTGTTT 24540

TGCGAATTGC GGCCGCCACC GCGGTGGAGC TCGAATTCAT TCCGATTAAT CGTGGCCTCT 24 600

TGCTCTTCAG GATGAAGAGC TATGTTTAAA CGTGCAAGCG CTACTAGACA ATTCAGTACA 24 660

TTAAAAACGT CCGCAATGTG TTATTAAGTT GTCTAAGCGT CAATTTGTTT ACACCACAAT 24 720

ATATCCTGCC ACCAGCCAGC CAACAGCTCC CCGACCGGCA GCTCGGCACA AAATCACCAC 24 780

TCGATACAGG CAGCCCATCA GTCCGGGACG GCGTCAGCGG GAGAGCCGTT GTAAGGCGGC 24840

AGACTTTGCT CATGTTACCG ATGCTATTCG GAAGAACGGC AACTAAGCTG CCGGGTTTGA 24 900

AACACGGATG ATCTCGCGGA GGGTAGCATG TTGATTGTAA CGATGACAGA GCGTTGCTGC 24 960

CTGTGATCAA ATATCATCTC CCTCGCAGAG ATCCGAATTA TCAGCCTTCT TATTCATTTC 25020

TCGCTTAACC GTGACAGGCT GTCGATCTTG AGAACTATGC CGACATAATA GGAAATCGCT 25080

GGATAAAGCC GCTGAGGAAG CTGAGTGGCG CTATTTCTTT AGAAGTGAAC GTTGACGATC 2514 0

GTCGACCGTA CCCCGATGAA TTAATTCGGA CGTACGTTCT GAACACAGCT GGATACTTAC 25200

TTGGGCGATT GTCATACATG ACATCAACAA TGTACCCGTT TGTGTAACCG TCTCTTGGAG 25260

GTTCGTATGA CACTAGTGGT TCCCCTCAGC TTGCGACTAG ATGTTGAGGC CTAACATTTT 25320

ATTAGAGAGC AGGCTAGTTG CTTAGATACA TGATCTTCAG GCCGTTATCT GTCAGGGCAA 25380

GCGAAAATTG GCCATTTATG ACGACCAATG CCCCGCAGAA GCTCCCATCT TTGCCGCCAT 25440

AGACGCCGCG CCCCCCTTTT GGGGTGTAGA ACATCCTTTT GCCAGATGTG GAAAAGAAGT 25500

TCGTTGTCCC ATTGTTGGCA ATGACGTAGT AGCCGGCGAA AGTGCGAGAC CCATTTGCGC 25560

TATATATAAG CCTACGATTT CCGTTGCGAC TATTGTCGTA ATTGGATGAA CTATTATCGT 25620

AGTTGCTCTC AGAGTTGTCG TAATTTGATG GACTATTGTC GTAATTGCTT ATGGAGTTGT 25680

CGTAGTTGCT TGGAGAAATG TCGTAGTTGG ATGGGGAGTA GTCATAGGGA AGACGAGCTT 25740

CATCCACTAA AACAATTGGC AGGTCAGCAA GTGCCTGCCC CGATGCCATC GCAAGTACGA 25800GGCTTAGAAC CACCTTCAAC AGATCGCGCATAAGCCGCGC CGCGAAGCGG CGTCGGCTTGCCTTGGTGAT CTCGCCTTTC ACGTAGTGAACCAAGCGATC TTCTTGTCCA AGATAAGCCTGGGCCGGCAG GCGCTCCATT GCCCAGTCGGTTACTGCGCT GTACCAAATG CGGGACAACGAGTCGGGCGG CGAGTTCCAT AGCGTTAAGGCAGGAACCGG ATCAAAGAGT TCCTCCGCCGTTGCTTTTGT CAGCAAGATA GCCAGATCAACAAGAATGTC ATTGCGCTGC CATTCTCCAAACGGAATGAT GTCGTCGTGC ACAACAATGGCTCCAGGGGA AGCCGAAGTT TCCAAAAGGTCAAGCCTTAC AGTCACCGTA ACCAGCAAATCCACTGCGGA GCCGTACAAA TGTACGGCCACGCCAACTAC CTCTGATAGT TGAGTCGATATTAACTCCTG AATTAAGCCG CGCCGCGAAGCATCCTGTGC TCCCGAGAAC CAGTACCAGTTAGTTTTATA CGTGAACAGG TCAATGCCGCTGCAGGCAGG TACATTGTTC GTTTGTGTCTGTGCCCACTT TTTCGCAAAT TCGATGAGACGCGACACAAC AATGTGTTCG ATAGAGGCTATCCCGACAAG CTCTTGGTCG ATGAATGCGCCATCCGAGAT GTAATCCTTC CGGTAGGGGCGGTCGGATAG GTGCACATCG AACACTTCACTTTCCGCCAC CTGCTCAGGG ATCACCGAAACGGATCGCAA ACCTGGCGCG GCTTTTGGCAGCTGCCACTT GTTAACCCTT TTGCCAGATTTCTTGGGTAA AAACTGGCCT AAAATTGCTGGGCTCGATGT CTATTGTAGA TATATGTAGTCGCGCGTTTC GGTGATGACG GTGAAAACCTAGCTTGTCTG TAAGCGGATG CCGGGAGCAGTGGCGGGTGT CGGGGCGCAG CCATGACCCACTTAACTATG CGGCATCAGA GCAGATTGTACCGCACAGAT GCGTAAGGAG AAAATACCGCGACTCGCTGC GCTCGGTCGT TCGGCTGCGGATACGGTTAT CCACAGAATC AGGGGATAACCAAAAGGCCA GGAACCGTAA AAAGGCCGCGCCTGACGAGC ATCACAAAAA TCGACGCTCATAAAGATACC AGGCGTTTCC CCCTGGAAGCCCGCTTACCG GATACCTGTC CGCCTTTCTCTCACGCTGTA GGTATCTCAG TTCGGTGTAGGAACCCCCCG TTCAGCCCGA CCGCTGCGCCCCGGTAAGAC ACGACTTATC GCCACTGGCAAGGTATGTAG GCGGTGCTAC AGAGTTCTTGAGGACAGTAT TTGGTATCTG CGCTCTGCTGAGCTCTTGAT CCGGCAAACA AACCACCGCTCAGATTACGC GCAGAAAAAA AGGATCTCAAGACGCTCAGT GGAACGAAAA CTCACGTTAAATCTTCACCT AGATCCTTTT AAATTAAAAAGAGTAAACTT GGTCTGACAG TTACCAATGCTGTCTATTTC GTTCATCCAT AGTTGCCTGAGAGGGCTTAC CATCTGGCCC CAGTGCTGCACCAGATTTAT CAGCAATAAA CCAGCCAGCCACTTTATCCG CCTCCATCCA GTCTATTAATCCAGTTAATA GTTTGCGCAA CGTTGTTGCCTTCCATTGTT CATTCCACGG ACAAAAACAGCGCTTTCAGC ACCTGTCGTT TCCTTTCTTTTTATGACGAA GAAGAACGGA AACGCCTTAACGCGAGGTCG CCGCCCCGTA AGCCGCCCCG

TAGTCTTCCC CAGCTCTCTA ACGCTTGAGT 258 60

AACGAATTGT TAGACATTAT TTGCCGACTA 25920

CAAATTCTTC CAACTGATCT GCGCGCGAGG 25980

GCCTAGCTTC AAGTATGACG GGCTGATACT 26040

CAGCGACATC CTTCGGCGCG ATTTTGCCGG 26100

TAAGCACTAC ATTTCGCTCA TCGCCAGCCC 26160

TTTCATTTAG CGCCTCAAAT AGATCCTGTT 26220

CTGGACCTAC CAAGGCAACG CTATGTTCTC 26280

TGTCGATCGT GGCTGGCTCG AAGATACCTG 26340

ATTGCAGTTC GCGCTTAGCT GGATAACGCC 264 00

TGACTTCTAC AGCGCGGAGA ATCTCGCTCT 264 60

CGTTGATCAA AGCTCGCCGC GTTGTTTCAT 26520

CAATATCACT GTGTGGCTTC AGGCCGCCAT 26580

GCAACGTCGG TTCGAGATGG CGCTCGATGA 26640

CTTCGGCGAT CACCGCTTCC CTCATGATGT 26700

CGGTGTCGGC TTGAATGAAT TGTTAGGCGT 2 67 60

ACATCGCTGT TTCGTTCGAG ACTTGAGGTC 26820

CGAGAGTAAA GCCACATTTT GCGTACAAAT 26880

CTAATCGTAT GCCAAGGAGC TGTCTGCTTA 26940

TGTGCGCGAC TCCTTTGCCT CGGTGCGTGT 27000

GATCGTTCCA TGTTGAGTTG AGTTCAATCT 27 060

CATAGCAAGC AGAGTCTTCA TCAGAGTCAT 27120

TCACACTTCT GGTAGATAGT TCAAAGCCTT 27180

GAACAATGAA ATGGTTCTCA GCATCCAATG 27240

TCTTCATATG ACGCCTAACG CCTGGCACAG 27300

CAAAAGGCGT GACAGGTTTG CGAATCCGTT 27360

TGGTAACTAT AATTTATGTT AGAGGCGAAG 27 420

GGGATTTCAG GAAAGTAAAC ATCACCTTCC 274 80

GTATCTACTT GATCGGGGGA TCTGCTGCCT 27 54 0

CTGACACATG CAGCTCCCGG AGACGGTCAC 27 600

ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT 27 660

GTCACGTAGC GATAGCGGAG TGTATACTGG 27 720

CTGAGAGTGC ACCATATGCG GTGTGAAATA 27 7 80

ATCAGGCGCT CTTCCGCTTC CTCGCTCACT 2784 0

CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 27 900

GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 27 960

TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 28020

AGTCAGAGGT GGCGAAACCC GACAGGACTA 28080

TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 2814 0

CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 28200

GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 28260

TTATCCGGTA ACTATCGTCT TGAGTCCAAC 28320

GCAGCCACTG GTAACAGGAT TAGCAGAGCG 28380

AAGTGGTGGC CTAACTACGG CTACACTAGA 28440

AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 28500

GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 28560

GAAGATCCTT TGATCTTTTC TACGGGGTCT 28 620

GGGATTTTGG TCATGAGATT ATCAAAAAGG 28 680

TGAAGTTTTA AATCAATCTA AAGTATATAT 28740

TTAATCAGTG AGGCACCTAT CTCAGCGATC 28800

CTCCCCGTCG TGTAGATAAC TACGATACGG 288 60

ATGATACCGC GAGACCCACG CTCACCGGCT 28 920

GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 28 980

TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 29040

ATTGCTGCAG GGGGGGGGGG GGGGGGGGAC 2 9100

AGAAAGGAAA CGACAGAGGC CAAAAAGCCT 29160

TCAGAGGGTA TTTTAAATAA AAACATTAAG 29220

ACCGGAAAAT TTTCATAAAT AGCGAAAACC 29280

TAACCTGTCG GATCACCGGA AAGGACCCGT 29340AAAGTGATAA TGATTATCAT CTACATATCA CAACGTGCGT GGAGGCCATC AAACCACGTC 29400

AAATAATCAA TTATGACGCA GGTATCGTAT TAATTGATCT GCATCAACTT AACGTAAAAA 294 60

CAACTTCAGA CAATACAAAT CAGCGACACT GAATACGGGG CAACCTCATG TCCCCCCCCC 29520

CCCCCCCCCT GCAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 29580

CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 29640

CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 29700

TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 2 97 60

TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 2 9820

CCCGGCGTCA ACACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 29880

TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 2 994 0

GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 30000

TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 30060

ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 30120

TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 30180

CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC 3024 0

CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAA GAATTCGGAG CTTTTGCCAT 30300

TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG 30360

AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG 304 20

ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT 304 80

TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG 3054 0

ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA 30600

CTTGACGGGA CGGCGGCTTT GTTGAATAAA TCGAACTTTT GCTGAGTTGA AGGATCAGAT 30660

CACGCATCTT CCCGACAACG CAGACCGTTC CGTGGCAAAG CAAAAGTTCA AAATCACCAA 30720

CTGGTCCACC TACAACAAAG CTCTCATCAA CCGTGGCTCC CTCACTTTCT GGCTGGATGA 30780

TGGGGCGATT CAGGCCTGGT ATGAGTCAGC AACACCTTCT TCACGAGGCA GACCTCAGCG 3084 0

CCAGAAGGCC GCCAGAGAGG CCGAGCGCGG CCGTGAGGCT TGGACGCTAG GGCAGGGCAT 30900

GAAAAAGCCC GTAGCGGGCT GCTACGGGCG TCTGACGCGG TGGAAAGGGG GAGGGGATGT 30960

TGTCTACATG GCTCTGCTGT AGTGAGTGGG TTGCGCTCCG GCAGCGGTCC TGATCAATCG 31020

TCACCCTTTC TCGGTCCTTC AACGTTCCTG ACAACGAGCC TCCTTTTCGC CAATCCATCG 31080

ACAATCACCG CGAGTCCCTG CTCGAACGCT GCGTCCGGAC CGGCTTCGTC GAAGGCGTCT 31140

ATCGCGGCCC GCAACAGCGG CGAGAGCGGA GCCTGTTCAA CGGTGCCGCC GCGCTCGCCG 31200

GCATCGCTGT CGCCGGCCTG CTCCTCAAGC ACGGCCCCAA CAGTGAAGTA GCTGATTGTC 31260

ATCAGCGCAT TGACGGCGTC CCCGGCCGAA AAACCCGCCT CGCAGAGGAA GCGAAGCTGC 31320

GCGTCGGCCG TTTCCATCTG CGGTGCGCCC GGTCGCGTGC CGGCATGGAT GCGCGCGCCA 31380

TCGCGGTAGG CGAGCAGCGC CTGCCTGAAG CTGCGGGCAT TCCCGATCAG AAATGAGCGC 314 4 0

CAGTCGTCGT CGGCTCTCGG CACCGAATGC GTATGATTCT CCGCCAGCAT GGCTTCGGCC 31500

AGTGCGTCGA GCAGCGCCCG CTTGTTCCTG AAGTGCCAGT AAAGCGCCGG CTGCTGAACC 31560

CCCAACCGTT CCGCCAGTTT GCGTGTCGTC AGACCGTCTA CGCCGACCTC GTTCAACAGG 31620

TCCAGGGCGG CACGGATCAC TGTATTCGGC TGCAACTTTG TCATGCTTGA CACTTTATCA 31680

CTGATAAACA TAATATGTCC ACCAACTTAT CAGTGATAAA GAATCCGCGC GTTCAATCGG 31740

ACCAGCGGAG GCTGGTCCGG AGGCCAGACG TGAAACCCAA CATACCCCTG ATCGTAATTC 31800

TGAGCACTGT CGCGCTCGAC GCTGTCGGCA TCGGCCTGAT TATGCCGGTG CTGCCGGGCC 318 60

TCCTGCGCGA TCTGGTTCAC TCGAACGACG TCACCGCCCA CTATGGCATT CTGCTGGCGC 31920

TGTATGCGTT GGTGCAATTT GCCTGCGCAC CTGTGCTGGG CGCGCTGTCG GATCGTTTCG 31980

GGCGGCGGCC AATCTTGCTC GTCTCGCTGG CCGGCGCCAC TGTCGACTAC GCCATCATGG 3204 0

CGACAGCGCC TTTCCTTTGG GTTCTCTATA TCGGGCGGAT CGTGGCCGGC ATCACCGGGG 32100

CGACTGGGGC GGTAGCCGGC GCTTATATTG CCGATATCAC TGATGGCGAT GAGCGCGCGC 32160

GGCACTTCGG CTTCATGAGC GCCTGTTTCG GGTTCGGGAT GGTCGCGGGA CCTGTGCTCG 32220

GTGGGCTGAT GGGCGGTTTC TCCCCCCACG CTCCGTTCTT CGCCGCGGCA GCCTTGAACG 32280

GCCTCAATTT CCTGACGGGC TGTTTCCTTT TGCCGGAGTC GCACAAAGGC GAACGCCGGC 32340

CGTTACGCCG GGAGGCTCTC AACCCGCTCG CTTCGTTCCG GTGGGCCCGG GGCATGACCG 32400

TCGTCGCCGC CCTGATGGCG GTCTTCTTCA TCATGCAACT TGTCGGACAG GTGCCGGCCG 324 60

CGCTTTGGGT CATTTTCGGC GAGGATCGCT TTCACTGGGA CGCGACCACG ATCGGCATTT 32520

CGCTTGCCGC ATTTGGCATT CTGCATTCAC TCGCCCAGGC AATGATCACC GGCCCTGTAG 32580

CCGCCCGGCT CGGCGAAAGG CGGGCACTCA TGCTCGGAAT GATTGCCGAC GGCACAGGCT 3264 0

ACATCCTGCT TGCCTTCGCG ACACGGGGAT GGATGGCGTT CCCGATCATG GTCCTGCTTG 32700

CTTCGGGTGG CATCGGAATG CCGGCGCTGC AAGCAATGTT GTCCAGGCAG GTGGATGAGG 327 60

AACGTCAGGG GCAGCTGCAA GGCTCACTGG CGGCGCTCAC CAGCCTGACC TCGATCGTCG 32820

GACCCCTCCT CTTCACGGCG ATCTATGCGG CTTCTATAAC AACGTGGAAC GGGTGGGCAT 32880GGATTGCAGG CGCTGCCCTC TACTTGCTCT GCCTGCCGGC GCTGCGTCGC GGGCTTTGGA 3294 0

GCGGCGCAGG GCAACGAGCC GATCGCTGAT CGTGGAAACG ATAGGCCTAT GCCATGCGGG 33000

TCAAGGCGAC TTCCGGCAAG CTATACGCGC CCTAGGAGTG CGGTTGGAAC GTTGGCCCAG 33060

CCAGATACTC CCGATCACGA GCAGGACGCC GATGATTTGA AGCGCACTCA GCGTCTGATC 33120

CAAGAACAAC CATCCTAGCA ACACGGCGGT CCCCGGGCTG AGAAAGCCCA GTAAGGAAAC 33180

AACTGTAGGT TCGAGTCGCG AGATCCCCCG GAACCAAAGG AAGTAGGTTA AACCCGCTCC 3324 0

GATCAGGCCG AGCCACGCCA GGCCGAGAAC ATTGGTTCCT GTAGGCATCG GGATTGGCGG 33300

ATCAAACACT AAAGCTACTG GAACGAGCAG AAGTCCTCCG GCCGCCAGTT GCCAGGCGGT 33360

AAAGGTGAGC AGAGGCACGG GAGGTTGCCA CTTGCGGGTC AGCACGGTTC CGAACGCCAT 33420

GGAAACCGCC CCCGCCAGGC CCGCTGCGAC GCCGACAGGA TCTAGCGCTG CGTTTGGTGT 334 80

CAACACCAAC AGCGCCACGC CCGCAGTTCC GCAAATAGCC CCCAGGACCG CCATCAATCG 33540

TATCGGGCTA CCTAGCAGAG CGGCAGAGAT GAACACGACC ATCAGCGGCT GCACAGCGCC 33600

TACCGTCGCC GCGACCCCGC CCGGCAGGCG GTAGACCGAA ATAAACAACA AGCTCCAGAA 33660

TAGCGAAATA TTAAGTGCGC CGAGGATGAA GATGCGCATC CACCAGATTC CCGTTGGAAT 33720

CTGTCGGACG ATCATCACGA GCAATAAACC CGCCGGCAAC GCCCGCAGCA GCATACCGGC 337 80

GACCCCTCGG CCTCGCTGTT CGGGCTCCAC GAAAACGCCG GACAGATGCG CCTTGTGAGC 33840

GTCCTTGGGG CCGTCCTCCT GTTTGAAGAC CGACAGCCCA ATGATCTCGC CGTCGATGTA 33900

GGCGCCGAAT GCCACGGCAT CTCGCAACCG TTCAGCGAAC GCCTCCATGG GCTTTTTCTC 33960

CTCGTGCTCG TAAACGGACC CGAACATCTC TGGAGCTTTC TTCAGGGCCG ACAATCGGAT 34 020

CTCGCGGAAA TCCTGCACGT CGGCCGCTCC AAGCCGTCGA ATCTGAGCCT TAATCACAAT 34 080

TGTCAATTTT AATCCTCTGT TTATCGGCAG TTCGTAGAGC GCGCCGTGCG TCCCGAGCGA 3414 0

TACTGAGCGA AGCAAGTGCG TCGAGCAGTG CCCGCTTGTT CCTGAAATGC CAGTAAAGCG 34 200

CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC 34 260

AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT 34 320

TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG 34 380

AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT 34 44 0

CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA 34 500

GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA 34 560

CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG 34 620

GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA 34 680

TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC 34 740

GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA 34 800

TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA 34 860

ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA 34 920

GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC 34 980

AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG 3504 0

GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA 35100

GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCGAGACGA 35160

CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG 35220

TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG 35280

CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC 35340

GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT 35400

TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT 354 60

GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC 35520

TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC 35580

TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG 35640

TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACT CTTCATTAAC CGCTATATCG 35700

AAAATTGCTT GCGGCTTGTT AGAATTGCCA TGACGTACCT CGGTGTCACG GGTAAGATTA 35760

CCGATAAACT GGAACTGATT ATGGCTCATA TCGAAAGTCT CCTTGAGAAA GGAGACTCTA 35820

GTTTAGCTAA ACATTGGTTC CGCTGTCAAG AACTTTAGCG GCTAAAATTT TGCGGGCCGC 35880

GACCAAAGGT GCGAGGGGCG GCTTCCGCTG TGTACAACCA GATATTTTTC ACCAACATCC 3594 0

TTCGTCTGCT CGATGAGCGG GGCATGACGA AACATGAGCT GTCGGAGAGG GCAGGGGTTT 36000

CAATTTCGTT TTTATCAGAC TTAACCAACG GTAAGGCCAA CCCCTCGTTG AAGGTGATGG 36060

AGGCCATTGC CGACGCCCTG GAAACTCCCC TACCTCTTCT CCTGGAGTCC ACCGACCTTG 36120

ACCGCGAGGC ACTCGCGGAG ATTGCGGGTC ATCCTTTCAA GAGCAGCGTG CCGCCCGGAT 36180

ACGAACGCAT CAGTGTGGTT TTGCCGTCAC ATAAGGCGTT TATCGTAAAG AAATGGGGCG 36240

ACGACACCCG AAAAAAGCTG CGTGGAAGGC TCTGACGCCA AGGGTTAGGG CTTGCACTTC 36300

CTTCTTTAGC CGCTAAAACG GCCCCTTCTC TGCGGGCCGT CGGCTCGCGC ATCATATCGA 36360

CATCCTCAAC GGAAGCCGTG CCGCGAATGG CATCGGGCGG GTGCGCTTTG ACAGTTGTTT 36420TCTATCAGAA CCCCTACGTC GTGCGGTTCG ATTAGCTGTT TGTCTTGCAG GCTAAACACT 36480

TTCGGTATAT CGTTTGCCTG TGCGATAATG TTGCTAATGA TTTGTTGCGT AGGGGTTACT 36540

GAAAAGTGAG CGGGAAAGAA GAGTTTCAGA CCATCAAGGA GCGGGCCAAG CGCAAGCTGG 36600

AACGCGACAT GGGTGCGGAC CTGTTGGCCG CGCTCAACGA CCCGAAAACC GTTGAAGTCA 36660

TGCTCAACGC GGACGGCAAG GTGTGGCACG AACGCCTTGG CGAGCCGATG CGGTACATCT 36720

GCGACATGCG GCCCAGCCAG TCGCAGGCGA TTATAGAAAC GGTGGCCGGA TTCCACGGCA 36780

AAGAGGTCAC GCGGCATTCG CCCATCCTGG AAGGCGAGTT CCCCTTGGAT GGCAGCCGCT 36840

TTGCCGGCCA ATTGCCGCCG GTCGTGGCCG CGCCAACCTT TGCGATCCGC AAGCGCGCGG 36900

TCGCCATCTT CACGCTGGAA CAGTACGTCG AGGCGGGCAT CATGACCCGC GAGCAATACG 36960

AGGTCATTAA AAGCGCCGTC GCGGCGCATC GAAACATCCT CGTCATTGGC GGTACTGGCT 37020

CGGGCAAGAC CACGCTCGTC AACGCGATCA TCAATGAAAT GGTCGCCTTC AACCCGTCTG 37080

AGCGCGTCGT CATCATCGAG GACACCGGCG AAATCCAGTG CGCCGCAGAG AACGCCGTCC 37140

AATACCACAC CAGCATCGAC GTCTCGATGA CGCTGCTGCT CAAGACAACG CTGCGTATGC 37200

GCCCCGACCG CATCCTGGTC GGTGAGGTAC GTGGCCCCGA AGCCCTTGAT CTGTTGATGG 37 260

CCTGGAACAC CGGGCATGAA GGAGGTGCCG CCACCCTGCA CGCAAACAAC CCCAAAGCGG 37320

GCCTGAGCCG GCTCGCCATG CTTATCAGCA TGCACCCGGA TTCACCGAAA CCCATTGAGC 37 380

CGCTGATTGG CGAGGCGGTT CATGTGGTCG TCCATATCGC CAGGACCCCT AGCGGCCGTC 374 40

GAGTGCAAGA AATTCTCGAA GTTCTTGGTT ACGAGAACGG CCAGTACATC ACCAAAACCC 37 500

TGTAAGGAGT ATTTCCAATG ACAACGGCTG TTCCGTTCCG TCTGACCATG AATCGCGGCA 37 560

TTTTGTTCTA CCTTGCCGTG TTCTTCGTTC TCGCTCTCGC GTTATCCGCG CATCCGGCGA 37 620

TGGCCTCGGA AGGCACCGGC GGCAGCTTGC CATATGAGAG CTGGCTGACG AACCTGCGCA 37 680

ACTCCGTAAC CGGCCCGGTG GCCTTCGCGC TGTCCATCAT CGGCATCGTC GTCGCCGGCG 37740

GCGTGCTGAT CTTCGGCGGC GAACTCAACG CCTTCTTCCG AACCCTGATC TTCCTGGTTC 37800

TGGTGATGGC GCTGCTGGTC GGCGCGCAGA ACGTGATGAG CACCTTCTTC GGTCGTGGTG 37860

CCGAAATCGC GGCCCTCGGC AACGGGGCGC TGCACCAGGT GCAAGTCGCG GCGGCGGATG 37 920

CCGTGCGTGC GGTAGCGGCT GGACGGCTCG CCTAATCATG GCTCTGCGCA CGATCCCCAT 37 980

CCGTCGCGCA GGCAACCGAG AAAACCTGTT CATGGGTGGT GATCGTGAAC TGGTGATGTT 3804 0

CTCGGGCCTG ATGGCGTTTG CGCTGATTTT CAGCGCCCAA GAGCTGCGGG CCACCGTGGT 38100

CGGTCTGATC CTGTGGTTCG GGGCGCTCTA TGCGTTCCGA ATCATGGCGA AGGCCGATCC 38160

GAAGATGCGG TTCGTGTACC TGCGTCACCG CCGGTACAAG CCGTATTACC CGGCCCGCTC 38220

GACCCCGTTC CGCGAGAACA CCAATAGCCA AGGGAAGCAA TACCGATGAT CCAAGCAATT 38280

GCGATTGCAA TCGCGGGCCT CGGCGCGCTT CTGTTGTTCA TCCTCTTTGC CCGCATCCGC 38340

GCGGTCGATG CCGAACTGAA ACTGAAAAAG CATCGTTCCA AGGACGCCGG CCTGGCCGAT 38400

CTGCTCAACT ACGCCGCTGT CGTCGATGAC GGCGTAATCG TGGGCAAGAA CGGCAGCTTT 384 60

ATGGCTGCCT GGCTGTACAA GGGCGATGAC AACGCAAGCA GCACCGACCA GCAGCGCGAA 38520

GTAGTGTCCG CCCGCATCAA CCAGGCCCTC GCGGGCCTGG GAAGTGGGTG GATGATCCAT 38580

GTGGACGCCG TGCGGCGTCC TGCTCCGAAC TACGCGGAGC GGGGCCTGTC GGCGTTCCCT 38640

GACCGTCTGA CGGCAGCGAT TGAAGAAGAG CGCTCGGTCT TGCCTTGCTC GTCGGTGATG 387 00

TACTTCACCA GCTCCGCGAA GTCGCTCTTC TTGATGGAGC GCATGGGGAC GTGCTTGGCA 387 60

ATCACGCGCA CCCCCCGGCC GTTTTAGCGG CTAAAAAAGT CATGGCTCTG CCCTCGGGCG 38820

GACCACGCCC ATCATGACCT TGCCAAGCTC GTCCTGCTTC TCTTCGATCT TCGCCAGCAG 38880

GGCGAGGATC GTGGCATCAC CGAACCGCGC CGTGCGCGGG TCGTCGGTGA GCCAGAGTTT 38 940

CAGCAGGCCG CCCAGGCGGC CCAGGTCGCC ATTGATGCGG GCCAGCTCGC GGACGTGCTC 39000

ATAGTCCACG ACGCCCGTGA TTTTGTAGCC CTGGCCGACG GCCAGCAGGT AGGCCGACAG 39060

GCTCATGCCG GCCGCCGCCG CCTTTTCCTC AATCGCTCTT CGTTCGTCTG GAAGGCAGTA 39120

CACCTTGATA GGTGGGCTGC CCTTCCTGGT TGGCTTGGTT TCATCAGCCA TCCGCTTGCC 39180

CTCATCTGTT ACGCCGGCGG TAGCCGGCCA GCCTCGCAGA GCAGGATTCC CGTTGAGCAC 39240

CGCCAGGTGC GAATAAGGGA CAGTGAAGAA GGAACACCCG CTCGCGGGTG GGCCTACTTC 39300

ACCTATCCTG CCCGGCTGAC GCCGTTGGAT ACACCAAGGA AAGTCTACAC GAACCCTTTG 39360

GCAAAATCCT GTATATCGTG CGAAAAAGGA TGGATATACC GAAAAAATCG CTATAATGAC 394 20

CCCGAAGCAG GGTTATGCAG CGGAAAAGCG CTGCTTCCCT GCTGTTTTGT GGAATATCTA 39480

CCGACTGGAA ACAGGCAAAT GCAGGAAATT ACTGAACTGA GGGGACAGGC GAGAGACGAT 3954 0

GCCAAAGAGC TACACCGACG AGCTGGCCGA GTGGGTTGAA TCCCGCGCGG CCAAGAAGCG 39600

CCGGCGTGAT GAGGCTGCGG TTGCGTTCCT GGCGGTGAGG GCGGATGTCG AGGCGGCGTT 39660

AGCGTCCGGC TATGCGCTCG TCACCATTTG GGAGCACATG CGGGAAACGG GGAAGGTCAA 39720

GTTCTCCTAC GAGACGTTCC GCTCGCACGC CAGGCGGCAC ATCAAGGCCA AGCCCGCCGA 39780

TGTGCCCGCA CCGCAGGCCA AGGCTGCGGA ACCCGCGCCG GCACCCAAGA CGCCGGAGCC 39840

ACGGCGGCCG AAGCAGGGGG GCAAGGCTGA AAAGCCGGCC CCCGCTGCGG CCCCGACCGG 39900

CTTCACCTTC AACCCAACAC CGGACAAAAA GGATCTACTG TAATGGCGAA AATTCACATG 39960GTTTTGCAGG GCAAGGGCGG GGTCGGCAAG TCGGCCATCG CCGCGATCAT TGCGCAGTAC 4 0020

AAGATGGACA AGGGGCAGAC ACCCTTGTGC ATCGACACCG ACCCGGTGAA CGCGACGTTC 40080

GAGGGCTACA AGGCCCTGAA CGTCCGCCGG CTGAACATCA TGGCCGGCGA CGAAATTAAC 4 014 0

TCGCGCAACT TCGACACCCT GGTCGAGCTG ATTGCGCCGA CCAAGGATGA CGTGGTGATC 40200

GACAACGGTG CCAGCTCGTT CGTGCCTCTG TCGCATTACC TCATCAGCAA CCAGGTGCCG 4 02 60

GCTCTGCTGC AAGAAATGGG GCATGAGCTG GTCATCCATA CCGTCGTCAC CGGCGGCCAG 40320

GCTCTCCTGG ACACGGTGAG CGGCTTCGCC CAGCTCGCCA GCCAGTTCCC GGCCGAAGCG 40380

CTTTTCGTGG TCTGGCTGAA CCCGTATTGG GGGCCTATCG AGCATGAGGG CAAGAGCTTT 40440

GAGCAGATGA AGGCGTACAC GGCCAACAAG GCCCGCGTGT CGTCCATCAT CCAGATTCCG 40500

GCCCTCAAGG AAGAAACCTA CGGCCGCGAT TTCAGCGACA TGCTGCAAGA GCGGCTGACG 40560

TTCGACCAGG CGCTGGCCGA TGAATCGCTC ACGATCATGA CGCGGCAACG CCTCAAGATC 40620

GTCCGGCGCG GCCTGTTTGA ACAGCTCGAC GCGGCGGCCG TGCTATGAGC GACCAGATTG 4 0680

AAGAGCTGAT CCGGGAGATT GCGGCCAAGC ACGGCATCGC CGTCGGCCGC GACGACCCGG 4 0740

TGCTGATCCT GCATACCATC AACGCCCGGC TCATGGCCGA CAGTGCGGCC AAGCAAGAGG 4 0800

AAATCCTTGC CGCGTTCAAG GAAGAGCTGG AAGGGATCGC CCATCGTTGG GGCGAGGACG 4 08 60

CCAAGGCCAA AGCGGAGCGG ATGCTGAACG CGGCCCTGGC GGCCAGCAAG GACGCAATGG 4 0920

CGAAGGTAAT GAAGGACAGC GCCGCGCAGG CGGCCGAAGC GATCCGCAGG GAAATCGACG 4 0980

ACGGCCTTGG CCGCCAGCTC GCGGCCAAGG TCGCGGACGC GCGGCGCGTG GCGATGATGA 41040

ACATGATCGC CGGCGGCATG GTGTTGTTCG CGGCCGCCCT GGTGGTGTGG GCCTCGTTAT 41100

GAATCGCAGA GGCGCAGATG AAAAAGCCCG GCGTTGCCGG GCTTTGTTTT TGCGTTAGCT 41160

GGGCTTGTTT GACAGGCCCA AGCTCTGACT GCGCCCGCGC TCGCGCTCCT GGGCCTGTTT 41220

CTTCTCCTGC TCCTGCTTGC GCATCAGGGC CTGGTGCCGT CGGGCTGCTT CACGCATCGA 41280

ATCCCAGTCG CCGGCCAGCT CGGGATGCTC CGCGCGCATC TTGCGCGTCG CCAGTTCCTC 41340

GATCTTGGGC GCGTGAATGC CCATGCCTTC CTTGATTTCG CGCACCATGT CCAGCCGCGT 41400

GTGCAGGGTC TGCAAGCGGG CTTGCTGTTG GGCCTGCTGC TGCTGCCAGG CGGCCTTTGT 414 60

ACGCGGCAGG GACAGCAAGC CGGGGGCATT GGACTGTAGC TGCTGCAAAC GCGCCTGCTG 41520

ACGGTCTACG AGCTGTTCTA GGCGGTCCTC GATGCGCTCC ACCTGGTCAT GCTTTGCCTG 4158 0

CACGTAGAGC GCAAGGGTCT GCTGGTAGGT CTGCTCGATG GGCGCGGATT CTAAGAGGGC 41640

CTGCTGTTCC GTCTCGGCCT CCTGGGCCGC CTGTAGCAAA TCCTCGCCGC TGTTGCCGCT 417 00

GGACTGCTTT ACTGCCGGGG ACTGCTGTTG CCCTGCTCGC GCCGTCGTCG CAGTTCGGCT 417 60

TGCCCCCACT CGATTGACTG CTTCATTTCG AGCCGCAGCG ATGCGATCTC GGATTGCGTC 41820

AACGGACGGG GCAGCGCGGA GGTGTCCGGC TTCTCCTTGG GTGAGTCGGT CGATGCCATA 41880

GCCAAAGGTT TCCTTCCAAA ATGCGTCCAT TGCTGGACCG TGTTTCTCAT TGATGCCCGC 41940

AAGCATCTTC GGCTTGACCG CCAGGTCAAG CGCGCCTTCA TGGGCGGTCA TGACGGACGC 4 2000

CGCCATGACC TTGCCGCCGT TGTTCTCGAT GTAGCCGCGT AATGAGGCAA TGGTGCCGCC 42060

CATCGTCAGC GTGTCATCGA CAACGATGTA CTTCTGGCCG GGGATCACCT CCCCCTCGAA 4 2120

AGTCGGGTTG AACGCCAGGC GATGATCTGA ACCGGCTCCG GTTCGGGCGA CCTTCTCCCG 4 2180

CTGCACAATG TCCGTTTCGA CCTCAAGGCC AAGGCGGTCG GCCAGAACGA CCGCCATCAT 4 2240

GGCCGGAATC TTGTTGTTCC CCGCCGCCTC GACGGCGAGG ACTGGAACGA TGCGGGGCTT 4 2300

GTCGTCGCCG ATCAGCGTCT TGAGCTGGGC AACAGTGTCG TCCGAAATCA GGCGCTCGAC 42360

CAAATTAAGC GCCGCTTCCG CGTCGCCCTG CTTCGCAGCC TGGTATTCAG GCTCGTTGGT 42420

CAAAGAACCA AGGTCGCCGT TGCGAACCAC CTTCGGGAAG TCTCCCCACG GTGCGCGCTC 42480

GGCTCTGCTG TAGCTGCTCA AGACGCCTCC CTTTTTAGCC GCTAAAACTC TAACGAGTGC 42540

GCCCGCGACT CAACTTGACG CTTTCGGCAC TTACCTGTGC CTTGCCACTT GCGTCATAGG 4 2600

TGATGCTTTT CGCACTCCCG ATTTCAGGTA CTTTATCGAA ATCTGACCGG GCGTGCATTA 42660

CAAAGTTCTT CCCCACCTGT TGGTAAATGC TGCCGCTATC TGCGTGGACG ATGCTGCCGT 4 2720

CGTGGCGCTG CGACTTATCG GCCTTTTGGG CCATATAGAT GTTGTAAATG CCAGGTTTCA 4 27 80

GGGCCCCGGC TTTATCTACC TTCTGGTTCG TCCATGCGCC TTGGTTCTCG GTCTGGACAA 42840

TTCTTTGCCC ATTCATGACC AGGAGGCGGT GTTTCATTGG GTGACTCCTG ACGGTTGCCT 4 2900

CTGGTGTTAA ACGTGTCCTG GTCGCTTGCC GGCTAAAAAA AAGCCGACCT CGGCAGTTCG 4 2 960

AGGCCGGCTT TCCCTAGAGC CGGGCGCGTC AAGGTTGTTC CATCTATTTT AGTGAACTGC 4 3020

GTTCGATTTA TCAGTTACTT TCCTCCCGCT TTGTGTTTCC TCCCACTCGT TTCCGCGTCT 43080

AGCCGACCCC TCAACATAGC GGCCTCTTCT TGGGCTGCCT TTGCCTCTTG CCGCGCTTCG 4 3140

TCACGCTCGG CTTGCACCGT CGTAAAGCGC TCGGCCTGCC TGGCCGCCTC TTGCGCCGCC 43200

AACTTCCTTT GCTCCTGGTG GGCCTCGGCG TCGGCCTGCG CCTTCGCTTT CACCGCTGCC 43260

AACTCCGTGC GCAAACTCTC CGCTTCGCGC CTGGTGGCGT CGCGCTCGCC GCGAAGCGCC 4 3320

TGCATTTCCT GGTTGGCCGC GTCCAGGGTC TTGCGGCTCT CTTCTTTGAA TGCGCGGGCG 43380

TCCTGGTGAG CGTAGTCCAG CTCGGCGCGC AGCTCCTGCG CTCGACGCTC CACCTCGTCG 4 3440

GCCCGCTGCG TCGCCAGCGC GGCCCGCTGC TCGGCTCCTG CCAGGGCGGT GCGTGCTTCG 43500GCCAGGGCTT GCCGCTGGCG TGCGGCCAGC TCGGCCGCCT CGGCGGCCTG CTGCTCTAGC 43560

AATGTAACGC GCGCCTGGGC TTCTTCCAGC TCGCGGGCCT GCGCCTCGAA GGCGTCGGCC 4 3620

AGCTCCCCGC GCACGGCTTC CAACTCGTTG CGCTCACGAT CCCAGCCGGC TTGCGCTGCC 43680

TGCAACGATT CATTGGCAAG GGCCTGGGCG GCTTGCCAGA GGGCGGCCAC GGCCTGGTTG 43740

CCGGCCTGCT GCACCGCGTC CGGCACCTGG ACTGCCAGCG GGGCGGCCTG CGCCGTGCGC 4 3800

TGGCGTCGCC ATTCGCGCAT GCCGGCGCTG GCGTCGTTCA TGTTGACGCG GGCGGCCTTA 43860

CGCACTGCAT CCACGGTCGG GAAGTTCTCC CGGTCGCCTT GCTCGAACAG CTCGTCCGCA 4 3920

GCCGCAAAAA TGCGGTCGCG CGTCTCTTTG TTCAGTTCCA TGTTGGCTCC GGTAATTGGT 43980

AAGAATAATA ATACTCTTAC CTACCTTATC AGCGCAAGAG TTTAGCTGAA CAGTTCTCGA 4 4 040

CTTAACGGCA GGTTTTTTAG CGGCTGAAGG GCAGGCAAAA AAAGCCCCGC ACGGTCGGCG 4 4100

GGGGCAAAGG GTCAGCGGGA AGGGGATTAG CGGGCGTCGG GCTTCTTCAT GCGTCGGGGC 44160

CGCGCTTCTT GGGATGGAGC ACGACGAAGC GCGCACGCGC ATCGTCCTCG GCCCTATCGG 4 4 220

CCCGCGTCGC GGTCAGGAAC TTGTCGCGCG CTAGGTCCTC CCTGGTGGGC ACCAGGGGCA 4 4280

TGAACTCGGC CTGCTCGATG TAGGTCCACT CCATGACCGC ATCGCAGTCG AGGCCGCGTT 4 4 340

CCTTCACCGT CTCTTGCAGG TCGCGGTACG CCCGCTCGTT GAGCGGCTGG TAACGGGCCA 4 4 400

ATTGGTCGTA AATGGCTGTC GGCCATGAGC GGCCTTTCCT GTTGAGCCAG CAGCCGACGA 44460

CGAAGCCGGC AATGCAGGCC CCTGGCACAA CCAGGCCGAC GCCGGGGGCA GGGGATGGCA 4 4 520

GCAGCTCGCC AACCAGGAAC CCCGCCGCGA TGATGCCGAT GCCGGTCAAC CAGCCCTTGA 4 4 580

AACTATCCGG CCCCGAAACA CCCCTGCGCA TTGCCTGGAT GCTGCGCCGG ATAGCTTGCA 44 640

ACATCAGGAG CCGTTTCTTT TGTTCGTCAG TCATGGTCCG CCCTCACCAG TTGTTCGTAT 4 4 700

CGGTGTCGGA CGAACTGAAA TCGCAAGAGC TGCCGGTATC GGTCCAGCCG CTGTCCGTGT 44760

CGCTGCTGCC GAAGCACGGC GAGGGGTCCG CGAACGCCGC AGACGGCGTA TCCGGCCGCA 4 4 820

GCGCATCGCC CAGCATGGCC CCGGTCAGCG AGCCGCCGGC CAGGTAGCCC AGCATGGTGC 4 4 880

TGTTGGTCGC CCCGGCCACC AGGGCCGACG TGACGAAATC GCCGTCATTC CCTCTGGATT 4 4 940

GTTCGCTGCT CGGCGGGGCA GTGCGCCGCG CCGGCGGCGT CGTGGATGGC TCGGGTTGGC 4 5000

TGGCCTGCGA CGGCCGGCGA AAGGTGCGCA GCAGCTCGTT ATCGACCGGC TGCGGCGTCG 4 5060

GGGCCGCCGC CTTGCGCTGC GGTCGGTGTT CCTTCTTCGG CTCGCGCAGC TTGAACAGCA 45120

TGATCGCGGA AACCAGCAGC AACGCCGCGC CTACGCCTCC CGCGATGTAG AACAGCATCG 4 5180

GATTCATTCT TCGGTCCTCC TTGTAGCGGA ACCGTTGTCT GTGCGGCGCG GGTGGCCCGC 4 5240

GCCGCTGTCT TTGGGGATCA GCCCTCGATG AGCGCGACCA GTTTCACGTC GGCAAGGTTC 45300

GCCTCGAACT CCTGGCCGTC GTCCTCGTAC TTCAACCAGG CATAGCCTTC CGCCGGCGGC 4 5360

CGACGGTTGA GGATAAGGCG GGCAGGGCGC TCGTCGTGCT CGACCTGGAC GATGGCCTTT 4 5420

TTCAGCTTGT CCGGGTCCGG CTCCTTCGCG CCCTTTTCCT TGGCGTCCTT ACCGTCCTGG 4 5480

TCGCCGTCCT CGCCGTCCTG GCCGTCGCCG GCCTCCGCGT CACGCTCGGC ATCAGTCTGG 4 554 0

CCGTTGAAGG CATCGACGGT GTTGGGATCG CGGCCCTTCT CGTCCAGGAA CTCGCGCAGC 4 5600

AGCTTGACCG TGCCGCGCGT GATTTCCTGG GTGTCGTCGT CAAGCCACGC CTCGACTTCC 45660

TCCGGGCGCT TCTTGAAGGC CGTCACCAGC TCGTTCACCA CGGTCACGTC GCGCACGCGG 4 5720

CCGGTGTTGA ACGCATCGGC GATCTTCTCC GGCAGGTCCA GCAGCGTGAC GTGCTGGGTG 4 5780

ATGAACGCCG GCGACTTGCC GATTTCCTTG GCGATATCGC CTTTCTTCTT GCCCTTCGCC 4 5840

AGCTCGCGGC CAATGAAGTC GGCAATTTCG CGCGGGGTCA GCTCGTTGCG TTGCAGGTTC 4 5900

TCGATAACCT GGTCGGCTTC GTTGTAGTCG TTGTCGATGA ACGCCGGGAT GGACTTCTTG 4 5960

CCGGCCCACT TCGAGCCACG GTAGCGGCGG GCGCCGTGAT TGATGATATA GCGGCCCGGC 4 6020

TGCTCCTGGT TCTCGCGCAC CGAAATGGGT GACTTCACCC CGCGCTCTTT GATCGTGGCA 4 6080

CCGATTTCCG CGATGCTCTC CGGGGAAAAG CCGGGGTTGT CGGCCGTCCG CGGCTGATGC 4 6140

GGATCTTCGT CGATCAGGTC CAGGTCCAGC TCGATAGGGC CGGAACCGCC CTGAGACGCC 4 6200

GCAGGAGCGT CCAGGAGGCT CGACAGGTCG CCGATGCTAT CCAACCCCAG GCCGGACGGC 4 62 60

TGCGCCGCGC CTGCGGCTTC CTGAGCGGCC GCAGCGGTGT TTTTCTTGGT GGTCTTGGCT 4 6320

TGAGCCGCAG TCATTGGGAA ATCTCCATCT TCGTGAACAC GTAATCAGCC AGGGCGCGAA 4 6380

CCTCTTTCGA TGCCTTGCGC GCGGCCGTTT TCTTGATCTT CCAGACCGGC ACACCGGATG 4 6440

CGAGGGCATC GGCGATGCTG CTGCGCAGGC CAACGGTGGC CGGAATCATC ATCTTGGGGT 4 6500

ACGCGGCCAG CAGCTCGGCT TGGTGGCGCG CGTGGCGCGG ATTCCGCGCA TCGACCTTGC 4 6560

TGGGCACCAT GCCAAGGAAT TGCAGCTTGG CGTTCTTCTG GCGCACGTTC GCAATGGTCG 4 6620

TGACCATCTT CTTGATGCCC TGGATGCTGT ACGCCTCAAG CTCGATGGGG GACAGCACAT 4 6680

AGTCGGCCGC GAAGAGGGCG GCCGCCAGGC CGACGCCAAG GGTCGGGGCC GTGTCGATCA 4 6740

GGCACACGTC GAAGCCTTGG TTCGCCAGGG CCTTGATGTT CGCCCCGAAC AGCTCGCGGG 4 6800

CGTCGTCCAG CGACAGCCGT TCGGCGTTCG CCAGTACCGG GTTGGACTCG ATGAGGGCGA 4 68 60

GGCGCGCGGC CTGGCCGTCG CCGGCTGCGG GTGCGGTTTC GGTCCAGCCG CCGGCAGGGA 4 6920

CAGCGCCGAA CAGCTTGCTT GCATGCAGGC CGGTAGCAAA GTCCTTGAGC GTGTAGGACG 4 6980

CATTGCCCTG GGGGTCCAGG TCGATCACGG CAACCCGCAA GCCGCGCTCG AAAAAGTCGA 4 7040AGGCAAGATG CACAAGGGTC GAAGTCTTGCAAGTTTTCAT CGTTTGGTTT CCTGTTTTTTGTACGCCTGA TGTTCCGGCA GAACCGCCGTGTCCTCGAAC GCGGCCCACA CGCGATGCACCAGCGACGTT GCGAACGTCG CCTGTGGCTTGATGGTCTGC TGCCCCACTT CCAGCCCCTGCCGTTTCTTC ATGGATAACA CCCATAATTTCAGCCGCCAG CACATGAGAG AAGTTTAGCTCGCTAAAACT CGTCCTTGGC GTAACAAAACGCCGCTTATG GCTCTGCACC CGGCTCCATCTTGCGGATCG ACACTGCCAG CCCAACAAAGATGATGCCGG CCACACCGGC CATCGCCCACTGGTACTGCT TCGCAATGCT GGACCTCGGCGCCGCTTCTC CCCTTGGCGT AAAACCCAGCGCTTTCCCGA CCACGACGCG CGCACCAGGCTGCGCAAGGA CATAATCAGC CGCCGACTTGCGCGCGAAAT CCTTGGCCTC CACGGCCGCCGGGCCGGCGT CGTGATCGCC GCCGAGAATGATGCTGACGG CTATCACCAT CATGCAGACGGCACGGCACC CGCGACCACT ATGCCAAGAATGAAGTCCGT GAATGCCCCG ACGGCCGAAGCCTCACTGCC CGGCACCTGG TCGCTGAATGTTCCGGGCGT CGCGCTCGGG CTGATCGCCCCAAGGACTGC CAGCGCTGCC ATTTTTGGGGCCTGGGGGGA TGGGAGGCCC GCGTTAGCGGCCCTTCGGCG TGCGCGGTCA ÇGCGCACAGGAATATTGGTT TAAAAGCAGG TTAAAAGACACCCTTGCAAA TGCTGGATTT TCTGCCTGTGCCTCATCTGT CAGCACTCTG CCCCTCAAGTGTCGCGCCCC TCAAGTGTCA ATACCGCAGGTGTGGGAAAC TCGCGTAAAA TCAGGCGTTTGTCGCCGGCC GAAATCGAGC CTGCCCCTCACCTCAAGTGT CAACGTCCGC CCCTCATCTGCCACAACGCC GGCGGCCGCG GTGTCTCGCAGCAGGGCCAT AGACGGCCGC CAGCCCAGCGAAGGCGCTGG AAGCCCCGTA GCGACGCGGACGATGCGCAG CACGACATAG CCGGTTCTCGCAAGTGTCAA TGAAAGTTTC CAACGCGAGCTGAGAGCTTT GTTGTAGGTG GACCAGTTGGGGTCTGCGTT GTCGGGAAGA TGCGTGATCTTTCAACAAAG CCACGTTGTG TCTCAAAATCTATCATCATG AACAATAAAA CTGTCTGCTTGCCATATTCA ACGGGAAAC

CGACGCCGCC TTTCTGGTTG GCCGTGACCACTTGGCGTCC GCTTCCCACT TCCGGACGATTACCCGCGCG TACCCCTCGG GCAAGTTCTTCGCTTGCGAC ACTGCGCCCC TGGTCAGTCCCCCATCGACT AAGACGCCCC GCGCTATCTCGATCGCCTCC TGGAACTGGC TTTCGGTAAGGCTCCGCGCC TTGGTTGAAC ATAGCGGTGAAAACATTTCT CGCACGTCAA CACCTTTAGCAAAAGCCCGG AAACCGGGCT TTCGTCTCTTACCAACAGGT CGCGCACGCG CTTCACTCGGCCGGTTGCCG CCGCCGCCAG GATCGCGCCGCAGGTCGCCG CCTTCCGGTT CCATTCCTGCTCACCATAGG CTGACCGCTC GATGGCGTATGCCGCAGGCG GCATTGCCAT GCTGCCCGCCTTGCGGTCCA GACCTTCGGC CACGGCGAGCGCTCCACGCG CCTCGATCAG CTCTTGCACTATGAATCGCG CACGCGGCGA AGGCTCCGCACCCTTCACCA AGTTCGACGA CACGAAAATCGATCGCACGA ACCCGCTGAA TTGAACACGATGCCCAAGGT AAAAATTGCC GGCCCCGCCATGAAGGGCAG GCCGCCACCC AGGCCGCCGCTCGATGCCAG CACCTGCGGC ACGTCAATGCATCCCGTTAC TGCCCCGATC CCGGCAATGGTGAGGCCGTT CGCGGCCGAG GGGCGCAGCCGCCGGGAGGG TTCGAGAAGG GGGGGCACCCGCGCAGCCCT GGTTAAAAAC AAGGTTTATAGGTTAGCGGT GGCCGAAAAA CGGGCGGAAAGACAGCCCCT CAAATGTCAA TAGGTGCGCCGTCAAGGATC GCGCCCCTCA TCTGTCAGTAGCACTTATCC CCAGGCTTGT CCACATCATCTCGCCGATTT GCGAGGCTGG CCAGCTCCACTCTGTCAACG CCGCGCCGGG TGAGTCGGCCTCAGTGAGGG CCAAGTTTTC CGCGAGGTATCACGGCTTCG ACGGCGTTTC TGGCGCGTTTGCGAGGGCAA CCAGCCCGGT GAGCGTCGGAGAGGGGCGAG ACAAGCCAAG GGCGCAGGCTCAAGGACGAG AATTTCCCTG CGGTGCCCCTCATTCGCGAG AGCCTTGAGT CCACGCTAGATGATTTTGAA CTTTTGCTTT GCCACGGAACGATCCTTCAA CTCAGCAAAA GTTCGATTTATCTGATGTTA CATTGCACAA GATAAAAATAACATAAACAG TAATACAAGG GGTGTTATGA

<210> 95

<211> 49015

<212> DNA

<213> VETOR

<400> 95

GTCTTGCTCG ACTCTAGAGCCGGGGAAGCT TACAATAATGTTTCGTCATA AATCCCGGCCGCGGAACGGG AATATCGAGAACAGGTACTC CAGCTGATTGTGCGAATGAT TACTTGAGCGAGTGCTACAA GGCACCTTTCAAATGGAGCG CAGTAGTCCACTCGCACAGC CTCCAGATCC

TCGTTCCTCG AGGCCTCGAGTGTGTTGTTA AGTCTTGTTGTCCGTAACCC AGCTTTGGGCTGCCGGGCTG AACGCTGCAGATTATCTGCT GAAGGGTCTTCGATCGGGCA TCCAATTTTCAGTAACGAGC GACCGTCGATTCGAGGGCGG CGAAAGCCTCGATCGAGGGT CTTCGGCGTA

GCCTCGAGGA ACGGTACCTGCCTGTCATCG TCTGACTGACAAGCTCACGG ATTTGATCCGTTCCAGCTTT CCCTTTCGGGGGTTCCACCT CCTGGCACAATCCCGTCAGG TGCGTGGTCACCGTCGCCGG GATACGGACAGCCAAAAGCA ATACGTTCATGGCAGATAGA AGCATGGATA

47100471604722047280473404740047460475204758047640477004776047820478804794048000480604812048180482404830048360484204848048540486004866048720487804884048900489604902049080491404920049260493204938049440495004956049579

60120180240300360420480540198020402100

CATTGCTTGA GAGTATTCCG ATGGACTGAA GTATGGCTTC CATCTTTTCT CGTGTGTCTG 600

CATCTATTTC GAGAAAGCCC CCGATGCGGC GCACCGCAAC GCGAATTGCC ATACTATCCG 660

AAAGTCCCAG CAGGCGCGCT TGATAGGAAA AGGTTTCATA CTCGGCCGAT CGCAGACGGG 720

CACTCACGAC CTTGAACCCT TCAACTTTCA GGGATCGATG CTGGTTGATG GTAGTCTCAC 780

TCGACGTGGC TCTGGTGTGT TTTGACATAG CTTCCTCCAA AGAAAGCGGA AGGTCTGGAT 840

ACTCCAGCAC GAAATGTGCC CGGGTAGACG GATGGAAGTC TAGCCCTGCT CAATATGAAA 900

TCAACAGTAC ATTTACAGTC AATACTGAAT ATACTTGCTA CATTTGCAAT TGTCTTATAA 960

CGAATGTGAA ATAAAAATAG TGTAACAACG CTTTTACTCA TCGATAATCA CAAAAACATT 1020

TATACGAACA AAAATACAAA TGCACTCCGG TTTCACAGGA TAGGCGGGAT CAGAATATGC 1080

AACTTTTGAC GTTTTGTTCT TTCAAAGGGG GTGCTGGCAA AACCACCGCA CTCATGGGCC 114 0

TTTGCGCTGC TTTGGCAAAT GACGGTAAAC GAGTGGCCCT CTTTGATGCC GACGAAAACC 1200

GGCCTCTGAC GCGATGGAGA GAAAACGCCT TACAAAGCAG TACTGGGATC CTCGCTGTGA 1260

AGTCTATTCC GCCGACGAAA TGCCCCTTCT TGAAGCAGCC TATGAAAATG CCGAGCTCGA 1320

AGGATTTGAT TATGCGTTGG CCGATACGCG TGGCGGCTCG AGCGAGCTCA ACAACACAAT 1380

CATCGCTAGC TCAAACCTGC TTCTGATCCC CACCATGCTA ACGCCGCTCG ACATCGATGA 14 40

GGCACTATCT ACCTACCGCT ACGTCATCGA GCTGCTGTTG AGTGAAAATT TGGCAATTCC 1500

TACAGCTGTT TTGCGCCAAC GCGTCCCGGT CGGCCGATTG ACAACATCGC AACGCAGGAT 1560

GTCAGAGACG CTAGAGAGCC TTCCAGTTGT ACCGTCTCCC ATGCATGAAA GAGATGCATT 1620

TGCCGCGATG AAAGAACGCG GCATGTTGCA TCTTACATTA CTAAACACGG GAACTGATCC 1680

GACGATGCGC CTCATAGAGA GGAATCTTCG GATTGCGATG GAGGAAGTCG TGGTCATTTC 17 40

GAAACTGATC AGCAAAATCT TGGAGGCTTG AAGATGGCAA TTCGCAAGCC CGCATTGTCG 1800

GTCGGCGAAG CACGGCGGCT TGCTGGTGCT CGACCCGAGA TCCACCATCC CAACCCGACA 1860

CTTGTTCCCC AGAAGCTGGA CCTCCAGCAC TTGCCTGAAA AAGCCGACGA GAAAGACCAG 1920CAACGTGAGC CTCTCGTCGC CGATCACATT TACAGTCCCG ATCGACAACT TAAGCTAACTGTGGATGCCC TTAGTCCACC TCCGTCCCCG AAAAAGCTCC AGGTTTTTCT TTCAGCGCGACCGCCCGCGC CTCAAGTGTC GAAAACATAT GACAACCTCG TTCGGCAATA CAGTCCCTCG

AAGTCGCTAC AAATGATTTT AAGGCGCGCG TTGGACGATT TCGAAAGCAT GCTGGCAGAT 2160

GGATCATTTC GCGTGGCCCC GAAAAGTTAT CCGATCCCTT CAACTACAGA AAAATCCGTT 2220

CTCGTTCAGA CCTCACGCAT GTTCCCGGTT GCGTTGCTCG AGGTCGCTCG AAGTCATTTT 2280

GATCCGTTGG GGTTGGAGAC CGCTCGAGCT TTCGGCCACA AGCTGGCTAC CGCCGCGCTC 234 0

GCGTCATTCT TTGCTGGAGA GAAGCCATCG AGCAATTGGT GAAGAGGGAC CTATCGGAAC 24 00

CCCTCACCAA ATATTGAGTG TAGGTTTGAG GCCGCTGGCC GCGTCCTCAG TCACCTTTTG 24 60

AGCCAGATAA TTAAGAGCCA AATGCAATTG GCTCAGGCTG CCATCGTCCC CCCGTGCGAA 2520

ACCTGCACGT CCGCGTCAAA GAAATAACCG GCACCTCTTG CTGTTTTTAT CAGTTGAGGG 2580

CTTGACGGAT CCGCCTCAAG TTTGCGGCGC AGCCGCAAAA TGAGAACATC TATACTCCTG 264 0

TCGTAAACCT CCTCGTCGCG TACTCGACTG GCAATGAGAA GTTGCTCGCG CGATAGAACG 2700

TCGCGGGGTT TCTCTAAAAA CGCGAGGAGA AGATTGAACT CACCTGCCGT AAGTTTCACC 2760

TCACCGCCAG CTTCGGACAT CAAGCGACGT TGCCTGAGAT TAAGTGTCCA GTCAGTAAAA 2820

CAAAAAGACC GTCGGTCTTT GGAGCGGACA ACGTTGGGGC GCACGCGCAA GGCAACCCGA 2880

ATGCGTGCAA GAAACTCTCT CGTACTAAAC GGCTTAGCGA TAAAATCACT TGCTCCTAGC 294 0

TCGAGTGCAA CAACTTTATC CGTCTCCTCA AGGCGGTCGC CACTGATAAT TATGATTGGA 3000

ATATCAGACT TTGCCGCCAG ATTTCGAACG ATCTCAAGCC CATCTTCACG ACCTAAATTT 3060

AGATCAACAA CCACGACATC GACCGTCGCG GAAGAGAGTA CTCTAGTGAA CTGGGTGCTG 3120

TCGGCTACCG CGGTCACTTT GAAGGCGTGG ATCGTAAGGT ATTCGATAAT AAGATGCCGC 3180

ATAGCGACAT CGTCATCGAT AAGAAGAACG TGTTTCAACG GCTCACCTTT CAATCTAAAA 3240

TCTGAACCCT TGTTCACAGC GCTTGAGAAA TTTTCACGTG AAGGATGTAC AATCATCTCC 3300

AGCTAAATGG GCAGTTCGTC AGAATTGCGG CTGACCGCGG ATGACGAAAA TGCGAACCAA 3360

GTATTTCAAT TTTATGACAA AAGTTCTCAA TCGTTGTTAC AAGTGAAACG CTTCGAGGTT 3420

ACAGCTACTA TTGATTAAGG AGATCGCCTA TGGTCTCGCC CCGGCGTCGT GCGTCCGCCG 3480

CGAGCCAGAT CTCGCCTACT TCATAAACGT CCTCATAGGC ACGGAATGGA ATGATGACAT 3540

CGATCGCCGT AGAGAGCATG TCAATCAGTG TGCGATCTTC CAAGCTAGCA CCTTGGGCGC 3600

TACTTTTGAC AAGGGAAAAC AGTTTCTTGA ATCCTTGGAT TGGATTCGCG CCGTGTATTG 3660

TTGAAATCGA TCCCGGATGT CCCGAGACGA CTTCACTCAG ATAAGCCCAT GCTGCATCGT 3720

CGCGCATCTC GCCAAGCAAT ATCCGGTCCG GCCGCATACG CAGACTTGCT TGGAGCAAGT 3780

GCTCGGCGCT CACAGCACCC AGCCCAGCAC CGTTCTTGGA GTAGAGTAGT CTAACATGAT 3840

TATCGTGTGG AATGACGAGT TCGAGCGTAT CTTCTATGGT GATTAGCCTT TCCTGGGGGG 3900

GGATGGCGCT GATCAAGGTC TTGCTCATTG TTGTCTTGCC GCTTCCGGTA GGGCCACATA 3960

GCAACATCGT CAGTCGGCTG ACGACGCATG CGTGCAGAAA CGCTTCCAAA TCCCCGTTGT 4 020

CAAAATGCTG AAGGATAGCT TCATCATCCT GATTTTGGCG TTTCCTTCGT GTCTGCCACT 4 080GGTTCCACCT CGAAGCATCA TAACGGGAGG AGACTTCTTT AAGACCAGAA ACACGCGAGC 4140

TTGGCCGTCG AATGGTCAAG CTGACGGTGC CCGAGGGAAC GGTCGGCGGC AGACAGATTT 4200

GTAGTCGTTC ACCACCAGGA AGTTCAGTGG CGCAGAGGGG GTTACGTGGT CCGACATCCT 4260

GCTTTCTCAG CGCGCCCGCT AAAATAGCGA TATCTTCAAG ATCATCATAA GAGACGGGCA 4320

AAGGCATCTT GGTAAAAATG CCGGCTTGGC GCACAAATGC CTCTCCAGGT CGATTGATCG 4 380

CAATTTCTTC AGTCTTCGGG TCATCGAGCC ATTCCAAAAT CGGCTTCAGA AGAAAGCGTA 4 440

GTTGCGGATC CACTTCCATT TACAATGTAT CCTATCTCTA AGCGGAAATT TGAATTCATT 4 500

AAGAGCGGCG GTTCCTCCCC CGCGTGGCGC CGCCAGTCAG GCGGAGCTGG TAAACACCAA 4 560

AGAAATCGAG GTCCCGTGCT ACGAAAATGG AAACGGTGTC ACCCTGATTC TTCTTCAGGG 4 620

TTGGCGGTAT GTTGATGGTT GCCTTAAGGG CTGTCTCAGT TGTCTGCTCA CCGTTATTTT 4 680

GAAAGCTGTT GAAGCTCATC CCGCCACCCG AGCTGCCGGC GTAGGTGCTA GCTGCCTGGA 47 40

AGGCGCCTTG AACAACACTC AAGAGCATAG CTCCGCTAAA ACGCTGCCAG AAGTGGCTGT 4 800

CGACCGAGCC CGGCAATCCT GAGCGACCGA GTTCGTCCGC GCTTGGCGAT GTTAACGAGA 4 8 60

TCATCGCATG GTCAGGTGTC TCGGCGCGAT CCCACAACAC AAAAACGCGC CCATCTCCCT 4 920

GTTGCAAGCC ACGCTGTATT TCGCCAACAA CGGTGGTGCC ACGATCAAGA AGCACGATAT 4 980

TGTTCGTTGT TCCACGAATA TCCTGAGGCA AGACACACTT TACATAGCCT GCCAAATTTG 5040

TGTCGATTGC GGTTTGCAAG ATGCACGGAA TTATTGTCCC TTGCGTTACC ATAAAATCGG 5100

GGTGCGGCAA GAGCGTGGCG CTGCTGGGCT GCAGCTCGGT GGGTTTCATA CGTATCGACA 5160

AATCGTTCTC GCCGGACACT TCGCCATTCG GCAAGGAGTT GTCGTCACGC TTGCCTTCTT 5220

GTCTTCGGCC CGTGTCGCCC TGAATGGCGC GTTTGCTGAC CCCTTGATCG CCGCTGCTAT 5280

ATGCAAAAAT CGGTGTTTCT TCCGGCCGTG GCTCATGCCG CTCCGGTTCG CCCCTCGGCG 534 0

GTAGAGGAGC AGCAGGCTGA ACAGCCTCTT GAACCGCTGG AGGATCCGGC GGCACCTCAA 54 00

TCGGAGCTGG ATGAAATGGC TTGGTGTTTG TTGCGATCAA AGTTGACGGC GATGCGTTCT 54 60

CATTCACCTT CTTTTGGCGC CCACCTAGCC AAATGAGGCT TAATGATAAC GCGAGAACGA 5520

CACCTCCGAC GATCAATTTC TGAGACCCCG AAAGACGCCG GCGATGTTTG TCGGAGACCA 5580

GGGATCCAGA TGCATCAACC TCATGTGCCG CTTGCTGACT ATCGTTATTC ATCCCTTCGC 5640

CCCCTTCAGG ACGCGTTTCA CATCGGGCCT CACCGTGCCC GTTTGCGGCC TTTGGCCAAC 5700

GGGATCGTAA GCGGTGTTCC AGATACATAG TACTGTGTGG CCATCCCTCA GACGCCAACC 57 60

TCGGGAAACC GAAGAAATCT CGACATCGCT CCCTTTAACT GAATAGTTGG CAACAGCTTC 5820

CTTGCCATCA GGATTGATGG TGTAGATGGA GGGTATGCGT ACATTGCCCG GAAAGTGGAA 5880

TACCGTCGTA AATCCATTGT CGAAGACTTC GAGTGGCAAC AGCGAACGAT CGCCTTGGGC 5940

GACGTAGTGC CAATTACTGT CCGCCGCACC AAGGGCTGTG ACAGGCTGAT CCAATAAATT 6000

CTCAGCTTTC CGTTGATATT GTGCTTCCGC GTGTAGTCTG TCCACAACAG CCTTCTGTTG 6060

TGCCTCCCTT CGCCGAGCCG CCGCATCGTC GGCGGGGTAG GCGAATTGGA CGCTGTAATA 6120

GAGATCGGGC TGCTCTTTAT CGAGGTGGGA CAGAGTCTTG GAACTTATAC TGAAAACATA 6180

ACGGCGCATC CCGGAGTCGC TTGCGGTTAG CACGATTACT GGCTGAGGCG TGAGGACCTG 624 0

GCTTGCCTTG AAAAATAGAT AATTTCCCCG CGGTAGGGCT GCTAGATCTT TGCTATTTGA 6300

AACGGCAACC GCTGTCACCG TTTCGTTCGT GGCGAATGTT ACGACCAAAG TAGCTCCAAC 6360

CGCCGTCGAG AGGCGCACCA CTTGATCGGG ATTGTAAGCC AAATAACGCA TGCGCGGATC 64 20

TAGCTTGCCC GCCATTGGAG TGTCTTCAGC CTCCGCACCA GTCGCAGCGG CAAATAAACA 64 80

TGCTAAAATG AAAAGTGCTT TTCTGATCAT GGTTCGCTGT GGCCTACGTT TGAAACGGTA 654 0

TCTTCCGATG TCTGATAGGA GGTGACAACC AGACCTGCCG GGTTGGTTAG TCTCAATCTG 6600

CCGGGCAAGC TGGTCACCTT TTCGTAGCGA ACTGTCGCGG TCCACGTACT CACCACAGGC 6660

ATTTTGCCGT CAACGACGAG GGTCCTTTTA TAGCGAATTT GCTGCGTGCT TGGAGTTACA 6720

TCATTTGAAG CGATGTGCTC GACCTCCACC CTGCCGCGTT TGCCAAGAAT GACTTGAGGC 6780

GAACTGGGAT TGGGATAGTT GAAGAATTGC TGGTAATCCT GGCGCACTGT TGGGGCACTG 6840

AAGTTCGATA CCAGGTCGTA GGCGTACTGA GCGGTGTCGG CATCATAACT CTCGCGCAGG 6900

CGAACGTACT CCCACAATGA GGCGTTAACG ACGGCCTCCT CTTGAGTTGC AGGCAATCGC 6960

GAGACAGACA CCTCGCTGTC AACGGTGCCG TCCGGCCGTA TCCATAGATA TACGGGCACA 7 020

AGCCTGCTCA ACGGCACCAT TGTGGCTATA GCGAACGCTT GAGCAACATT TCCCAAAATC 7 080

GCGATAGCTG CGACAGCTGC AATGAGTTTG GAGAGACGTC GCGCCGATTT CGCTCGCGCG 7140

GTTTGAAAGG CTTCTACTTC CTTATAGTGC TCGGCAAGGC TTTCGCGCGC CACTAGCATG 7200

GCATATTCAG GCCCCGTCAT AGCGTCCACC CGAATTGCCG AGCTGAAGAT CTGACGGAGT 7260

AGGCTGCCAT CGCCCCACAT TCAGCGGGAA GATCGGGCCT TTGCAGCTCG CTAATGTGTC 7320

GTTTGTCTGG CAGCCGCTCA AAGCGACAAC TAGGCACAGC AGGCAATACT TCATAGAATT 7380

CTCCATTGAG GCGAATTTTT GCGCGACCTA GCCTCGCTCA ACCTGAGCGA AGCGACGGTA 7 4 40

CAAGCTGCTG GCAGATTGGG TTGCGCCGCT CCAGTAACTG CCTCCAATGT TGCCGGCGAT 7500

CGCCGGCAAA GCGACAATGA GCGCATCCCC TGTCAGAAAA AACATATCGA GTTCGTAAAG 7560

ACCAATGATC TTGGCCGCGG TCGTACCGGC GAAGGTGATT ACACCAAGCA TAAGGGTGAG 7620CGCAGTCGCT TCGGTTAGGA TGACGATCGT TGCCACGAGG TTTAAGAGGA GAAGCAAGAG 7 680

ACCGTAGGTG ATAAGTTGCC CGATCCACTT AGCTGCGATG TCCCGCGTGC GATCAAAAAT 774 0

ATATCCGACG AGGATCAGAG GCCCGATCGC GAGAAGCACT TTCGTGAGAA TTCCAACGGC 7 800

GTCGTAAACT CCGAAGGCAG ACCAGAGCGT GCCGTAAAGG ACCCACTGTG CCCCTTGGAA 7 860

AGCAAGGATG TCCTGGTCGT TCATCGGACC GATTTCGGAT GCGATTTTCT GAAAAACGGC 7 920

CTGGGTCACG GCGAACATTG TATCCAACTG TGCCGGAACA GTCTGCAGAG GCAAGCCGGT 7 980

TACACTAAAC TGCTGAACAA AGTTTGGGAC CGTCTTTTCG AAGATGGAAA CCACATAGTC 8040

TTGGTAGTTA GCCTGCCCAA CAATTAGAGC AACAACGATG GTGACCGTGA TCACCCGAGT 8100

GATACCGCTA CGGGTATCGA CTTCGCCGCG TATGACTAAA ATACCCTGAA CAATAATCCA 8160

AAGAGTGACA CAGGCGATCA ATGGCGCACT CACCGCCTCC TGGATAGTCT CAAGCATCGA 8220

GTCCAAGCCT GTCGTGAAGG CTACATCGAA GATCGTATGA ATGGCCGTAA ACGGCGCCGG 8280

AATCGTGAAA TTCATCGATT GGACCTGAAC TTGACTGGTT TGTCGCATAA TGTTGGATAA 834 0

AATGAGCTCG CATTCGGCGA GGATGCGGGC GGATGAACAA ATCGCCCAGC CTTAGGGGAG 8400

GGCACCAAAG ATGACAGCGG TCTTTTGATG CTCCTTGCGT TGAGCGGCCG CCTCTTCCGC 84 60

CTCGTGAAGG CCGGCCTGCG CGGTAGTCAT CGTTAATAGG CTTGTCGCCT GTACATTTTG 8520

AATCATTGCG TCATGGATCT GCTTGAGAAG CAAACCATTG GTCACGGTTG CCTGCATGAT 8580

ATTGCGAGAT CGGGAAAGCT GAGCAGACGT ATCAGCATTC GCCGTCAAGC GTTTGTCCAT 8 640

CGTTTCCAGA TTGTCAGCCG CAATGCCAGC GCTGTTTGCG GAACCGGTGA TCTGCGATCG 8700

CAACAGGTCC GCTTCAGCAT CACTACCCAC GACTGCACGA TCTGTATCGC TGGTGATCGC 87 60

ACGTGCCGTG GTCGACATTG GCATTCGCGG CGAAAACATT TCATTGTCTA GGTCCTTCGT 8820

CGAAGGATAC TGATTTTTCT GGTTGAGCGA AGTCAGTAGT CCAGTAACGC CGTAGGCCGA 8880

CGTCAACATC GTAACCATCG CTATAGTCTG AGTGAGATTC TCCGCAGTCG CGAGCGCAGT 8 94 0

CGCGAGCGTC TCAGCCTCCG TTGCCGGGTC GCTAACAACA AACTGCGCCC GCGCGGGCTG 9000

AATATATAGA AAGCTGCAGG TCAAAACTGT TGCAATAAGT TGCGTCGTCT TCATCGTTTC 9060

CTACCTTATC AATCTTCTGC CTCGTGGTGA CGGGCCATGA ATTCGCTGAG CCAGCCAGAT 9120

GAGTTGCCTT CTTGTGCCTC GCGTAGTCGA GTTGCAAAGC GCACCGTGTT GGCACGCCCC 9180

GAAAGCACGG CGACATATTC ACGCATATCC CGCAGATCAA ATTCGCAGAT GACGCTTCCA 924 0

CTTTCTCGTT TAAGAAGAAA CTTACGGCTG CCGACCGTCA TGTCTTCACG GATCGCCTGA 9300

AATTCCTTTT CGGTACATTT CAGTCCATCG ACATAAGCCG ATCGATCTGC GGTTGGTGAT 9360

GGATAGAAAA TCTTCGTCAT ACATTGCGCA ACCAAGCTGG CTCCTAGCGG CGATTCCAGA 9420

ACATGCTCTG GTTGCTGCGT TGCCAGTATT AGCATCCCGT TGTTTTTTCG AACGGTCAGG 9480

AGGAATTTGT CGACGACAGT CGAAAATTTA GGGTTTAACA AATAGGCGCG AAACTCATCG 954 0

CAGCTCATCA CAAAACGGCG GCCGTCGATC ATGGCTCCAA TCCGATGCAG GAGATATGCT 9600

GCAGCGGGAG CGCATACTTC CTCGTATTCG AGAAGATGCG TCATGTCGAA GCCGGTAATC 9660

GACGGATCTA ACTTTACTTC GTCAACTTCG CCGTCAAATG CCCAGCCAAG CGCATGGCCC 9720

CGGCACCAGC GTTGGAGCCG CGCTCCTGCG CCTTCGGCGG GCCCATGCAA CAAAAATTCA 9780

CGTAACCCCG CGATTGAACG CATTTGTGGA TCAAACGAGA GCTGACGATG GATACCACGG 984 0

ACCAGACGGC GGTTCTCTTC CGGAGAAATC CCACCCCGAC CATCACTCTC GATGAGAGCC 9900

ACGATCCATT CGCGCAGAAA ATCGTGTGAG GCTGCTGTGT TTTCTAGGCC ACGCAACGGC 9960

GCCAACCCGC TGGGTGTGCC TCTGTGAAGT GCCAAATATG TTCCTCCTGT GGCGCGAACC 10020

AGCAATTCGC CACCCCGGTC CTTGTCAAAG AACACGACCG TACCTGCACG GTCGACCATG 10080

CTCTGTTCGA GCATGGCTAG AACAAACATC ATGAGCGTCG TCTTACCCCT CCCGATAGGC 10140

CCGAATATTG CCGTCATGCC AACATCGTGC TCATGCGGGA TATAGTCGAA AGGCGTTCCG 10200

CCATTGGTAC GAAATCGGGC AATCGCGTTG CCCCAGTGGC CTGAGCTGGC GCCCTCTGGA 10260

AAGTTTTCGA AAGAGACAAA CCCTGCGAAA TTGCGTGAAG TGATTGCGCC AGGGCGTGTG 10320

CGCCACTTAA AATTCCCCGG CAATTGGGAC CAATAGGCCG CTTCCATACC AATACCTTCT 10380

TGGACAACCA CGGCACCTGC ATCCGCCATT CGTGTCCGAG CCCGCGCGCC CCTGTCCCCA 10440

AGACTATTGA GATCGTCTGC ATAGACGCAA AGGCTCAAAT GATGTGAGCC CATAACGAAT 10500

TCGTTGCTCG CAAGTGCGTC CTCAGCCTCG GATAATTTGC CGATTTGAGT CACGGCTTTA 10560

TCGCCGGAAC TCAGCATCTG GCTCGATTTG AGGCTAAGTT TCGCGTGCGC TTGCGGGCGA 10620

GTCAGGAACG AAAAACTCTG CGTGAGAACA AGTGGAAAAT CGAGGGATAG CAGCGCGTTG 10680

AGCATGCCCG GCCGTGTTTT TGCAGGGTAT TCGCGAAACG AATAGATGGA TCCAACGTAA 10740

CTGTCTTTTG GCGTTCTGAT CTCGAGTCCT CGCTTGCCGC AAATGACTCT GTCGGTATAA 10800

ATCGAAGCGC CGAGTGAGCC GCTGACGACC GGAACCGGTG TGAACCGACC AGTCATGATC 10860

AACCGTAGCG CTTCGCCAAT TTCGGTGAAG AGCACACCCT GCTTCTCGCG GATGCCAAGA 10920

CGATGCAGGC CATACGCTTT AAGAGAGCCA GCGACAACAT GCCAAAGATC TTCCATGTTC 10980

CTGATCTGGC CCGTGAGATC GTTTTCCCTT TTTCCGCTTA GCTTGGTGAA CCTCCTCTTT 11040

ACCTTCCCTA AAGCCGCCTG TGGGTAGACA ATCAACGTAA GGAAGTGTTC ATTGCGGAGG 11100

AGTTGGCCGG AGAGCACGCG CTGTTCAAAA GCTTCGTTCA GGCTAGCGGC GAAAACACTA 11160CGGAAGTGTC GCGGCGCCGA TGATGGCACG TCGGCATGAC GTACGAGGTG AGCATATATT 11220

GACACATGAT CATCAGCGAT ATTGCGCAAC AGCGTGTTGA ACGCACGACA ACGCGCATTG 11280

CGCATTTCAG TTTCCTCAAG CTCGAATGCA ACGCCATCAA TTCTCGCAAT GGTCATGATC 11340

GATCCGTCTT CAAGAAGGAC GATATGGTCG CTGAGGTGGC CAATATAAGG GAGATAGATC 11400

TCACCGGATC TTTCGGTCGT TCCACTCGCG CCGAGCATCA CACCATTCCT CTCCCTCGTG 11460

GGGGAACCCT AATTGGATTT GGGCTAACAG TAGCGCCCCC CCAAACTGCA CTATCAATGC 11520

TTCTTCCCGC GGTCCGCAAA AATAGCAGGA CGACGCTCGC CGCATTGTAG TCTCGCTCCA 11580

CGATGAGCCG GGCTGCAAAC CATAACGGCA CGAGAACGAC TTCGTAGAGC GGGTTCTGAA 11640

CGATAACGAT GACAAAGCCG GCGAACATCA TGAATAACCC TGCCAATGTC AGTGGCACCC 11700

CAAGAAACAA TGCGGGCCGT GTGGCTGCGA GGTAAAGGGT CGATTCTTCC AAACGATCAG 11760

CCATCAACTA CCGCCAGTGA GCGTTTGGCC GAGGAAGCTC GCCCCAAACA TGATAACAAT 11820

GCCGCCGACG ACGCCGGCAA CCAGCCCAAG CGAAGCCCGC CCGAACATCC AGGAGATCCC 11880

GATAGCGACA ATGCCGAGAA CAGCGAGTGA CTGGCCGAAC GGACCAAGGA TAAACGTGCA 11940

TATATTGTTA ACCATTGTGG CGGGGTCAGT GCCGCCACCC GCAGATTGCG CTGCGGCGGG 12000

TCCGGATGAG GAAATGCTCC ATGCAATTGC ACCGCACAAG CTTGGGGCGC AGCTCGATAT 12060

CACGCGCATC ATCGCATTCG AGAGCGAGAG GCGATTTAGA TGTAAACGGT ATCTCTCAAA 12120

GCATCGCATC AATGCGCACC TCCTTAGTAT AAGTCGAATA AGACTTGATT GTCGTCTGCG 12180

GATTTGCCGT TGTCCTGGTG TGGCGGTGGC GGAGCGATTA AACCGCCAGC GCCATCCTCC 12240

TGCGAGCGGC GCTGATATGA CCCCCAAACA TCCCACGTCT CTTCGGATTT TAGCGCCTCG 12300

TGATCGTCTT TTGGAGGCTC GATTAACGCG GGCACCAGCG ATTGAGCAGC TGTTTCAACT 12360

TTTCGCACGT AGCCGTTTGC AAAACCGCCG ATGAAATTAC CGGTGTTGTA AGCGGAGATC 12420

GCCCGACGAA GCGCAAATTG CTTCTCGTCA ATCGTTTCGC CGCCTGCATA ACGACTTTTC 12480

AGCATGTTTG CAGCGGCAGA TAATGATGTG CACGCCTGGA GCGCACCGTC AGGTGTCAGA 12540

CCGAGCATAG AAAAATTTCG AGAGTTTATT TGCATGAGGC CAACATCCAG CGAATGCCGT 12600

GCATCGAGAC GGTGCCTGAC GACTTGGGTT GCTTGGCTGT GATCTTGCCA GTGAAGCGTT 12660

TCGCCGGTCG TGTTGTCATG AATCGCTAAA GGATCAAAGC GACTCTCCAC CTTAGCTATC 12720

GCCGCAAGCG TAGATGTCGC AACTGATGGG GCACACTTGC GAGCAACATG GTCAAACTCA 12780

GCAGATGAGA GTGGCGTGGC AAGGCTCGAC GAACAGAAGG AGACCATCAA GGCAAGAGAA 12840

AGCGACCCCG ATCTCTTAAG CATACCTTAT CTCCTTAGCT CGCAACTAAC ACCGCCTCTC 12900

CCGTTGGAAG AAGTGCGTTG TTTTATGTTG AAGATTATCG GGAGGGTCGG TTACTCGAAA 12960

ATTTTCAATT GCTTCTTTAT GATTTCAATT GAAGCGAGAA ACCTCGCCCG GCGTCTTGGA 13020

ACGCAACATG GACCGAGAAC CGCGCATCCA TGACTAAGCA ACCGGATCGA CCTATTCAGG 13080

CCGCAGTTGG TCAGGTCAGG CTCAGAACGA AAATGCTCGG CGAGGTTACG CTGTCTGTAA 13140

ACCCATTCGA TGAACGGGAA GCTTCCTTCC GATTGCTCTT GGCAGGAATA TTGGCCCATG 13200

CCTGCTTGCG CTTTGCAAAT GCTCTTATCG CGTTGGTATC ATATGCCTTG TCCGCCAGCA 13260

GAAACGCACT CTAAGCGATT ATTTGTAAAA ATGTTTCGGT CATGCGGCGG TCATGGGCTT 13320

GACCCGCTGT CAGCGCAAGA CGGATCGGTC AACCGTCGGC ATCGACAACA GCGTGAATCT 13380

TGGTGGTCAA ACCGCCACGG GAACGTCCCA TACAGCCATC GTCTTGATCC CGCTGTTTCC 13440

CGTCGCCGCA TGTTGGTGGA CGCGGACACA GGAACTGTCA ATCATGACGA CATTCTATCG 13500

AAAGCCTTGG AAATCACACT CAGAATATGA TCCCAGACGT CTGCCTCACG CCATCGTACA 13560

AAGCGATTGT AGCAGGTTGT ACAGGAACCG TATCGATCAG GAACGTCTGC CCAGGGCGGG 13620

CCCGTCCGGA AGCGCCACAA GATGACATTG ATCACCCGCG TCAACGCGCG GCACGCGACG 13680

CGGCTTATTT GGGAACAAAG GACTGAACAA CAGTCCATTC GAAATCGGTG ACATCAAAGC 13740

GGGGACGGGT TATCAGTGGC CTCCAAGTCA AGCCTCAATG AATCAAAATC AGACCGATTT 13800

GCAAACCTGA TTTATGAGTG TGCGGCCTAA ATGATGAAAT CGTCCTTCTA GATCGCCTCC 13860

GTGGTGTAGC AACACCTCGC AGTATCGCCG TGCTGACCTT GGCCAGGGAA TTGACTGGCA 13920

AGGGTGCTTT CACATGACCG CTCTTTTGGC CGCGATAGAT GATTTCGTTG CTGCTTTGGG 13980

CACGTAGAAG GAGAGAAGTC ATATCGGAGA AATTCCTCCT GGCGCGAGAG CCTGCTCTAT 14040

CGCGACGGCA TCCCACTGTC GGGAACAGAC CGGATCATTC ACGAGGCGAA AGTCGTCAAC 14100

ACATGCGTTA TAGGCATCTT CCCTTGAAGG ATGATCTTGT TGCTGCCAAT CTGGAGGTGC 14160

GGCAGCCGCA GGCAGATGCG ATCTCAGCGC AACTTGCGGC AAAACATCTC ACTCACCTGA 14220

AAACCACTAG CGAGTCTCGC GATCAGACGA AGGCCTTTTA CTTAACGACA CAATATCCGA 14280

TGTCTGCATC ACAGGCGTCG CTATCCCAGT CAATACTAAA GCGGTGCAGG AACTAAAGAT 14340

TACTGATGAC TTAGGCGTGC CACGAGGCCT GAGACGACGC GCGTAGACAG TTTTTTGAAA 14400

TCATTATCAA AGTGATGGCC TCCGCTGAAG CCTATCACCT CTGCGCCGGT CTGTCGGAGA 14460

GATGGGCAAG CATTATTACG GTCTTCGCGC CCGTACATGC ATTGGACGAT TGCAGGGTCA 14520

ATGGATCTGA GATCATCCAG AGGATTGCCG CCCTTACCTT CCGTTTCGAG TTGGAGCCAG 14580

CCCCTAAATG AGACGACATA GTCGACTTGA TGTGACAATG CCAAGAGAGA GATTTGCTTA 14640

ACCCGATTTT TTTGCTCAAG CGTAAGCCTA TTGAAGCTTG CCGGCATGAC GTCCGCGCCG 14700AAAGAATATC CTACAAGTAA AACATTCTGC ACACCGAAAT GCTTGGTGTA GACATCGATT 14760

ATGTGACCAA GATCCTTAGC AGTTTCGCTT GGGGACCGCT CCGACCAGAA ATACCGAAGT 14 820

GAACTGACGC CAATGACAGG AATCCCTTCC GTCTGCAGAT AGGTACCATC GATAGATCTG 14 880

CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 14 940

GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 15000

GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA 15060

TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 15120

GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG 15180

CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 1524 0

GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 15300

GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 15360

CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 15420

GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 15480

ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 1554 0

CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 15600

GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 15660

TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 15720

AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 15780

ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 15840

GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 15900

AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 15960

GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 16020

AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 16080

ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 1614 0

GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 16200

ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 162 60

CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 16320

CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 16380

AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGGGG GGGGGGGGGG 16440

GGGTTCCATT GTTCATTCCA CGGACAAAAA CAGAGAAAGG AAACGACAGA GGCCAAAAAG 16500

CTCGCTTTCA GCACCTGTCG TTTCCTTTCT TTTCAGAGGG TATTTTAAAT AAAAACATTA 16560

AGTTATGACG AAGAAGAACG GAAACGCCTT AAACCGGAAA ATTTTCATAA ATAGCGAAAA 16620

CCCGCGAGGT CCCTGTCGGA TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT 16680

ACATATCACA ACGTGCGTGG AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG 16740

TATCGTATTA ATTGATCTGC ATCAACTTAA CGTAAAAACA ACTTCAGACA ATACAAATCA 16800

GCGACACTGA ATACGGGGCA ACCTCATGTC CCCCCCCCCC CCCCCCCTGC AGGCATCGTG 16860

GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 16920

GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 16980

GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 17 040

CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 17100

TTCTGAGAAT AGTGTATGCG■GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT 17160

ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 17220

AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 17280

AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 17340

CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 17 4 00

CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 17 4 60

GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 17 520

CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 17 580

AGGCCCTTTC GTCTTCAAGA ATTGGTCGAC GATCTTGCTG CGTTCGGATA TTTTCGTGGA 17 640

GTTCCCGCCA CAGACCCGGA TTGAAGGCGA GATCCAGCAA CTCGCGCCAG ATCATCCTGT 17700

GACGGAACTT TGGCGCGTGA TGACTGGCCA GGACGTCGGC CGAAAGAGCG ACAAGCAGAT 177 60

CACGCTTTTC GACAGCGTCG GATTTGCGAT CGAGGATTTT TCGGCGCTGC GCTACGTCCG 17820

CGACCGCGTT GAGGGATCAA GCCACAGCAG CCCACTCGAC CTTCTAGCCG ACCCAGACGA 17880

GCCAAGGGAT CTTTTTGGAA TGCTGCTCCG TCGTCAGGCT TTCCGACGTT TGGGTGGTTG 17940

AACAGAAGTC ATTATCGTAC GGAATGCCAA GCACTCCCGA GGGGAACCCT GTGGTTGGCA 18000

TGCACATACA AATGGACGAA CGGATAAACC TTTTCACGCC CTTTTAAATA TCCGTTATTC 18060

TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT 18120

TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT 18180

ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA 1824 0CGTTGAAGGA GCCACTCAGC AAGCTGGTAC GATTGTAATA CGACTCACTA TAGGGCGAAT 18300

TGAGCGCTGT TTAAACGCTC TTCAACTGGA AGAGCGGTTA CCAGAGCTGG TCACCTTTGT 18360

CCACCAAGAT GGAACTGCGG CCGCTCATTA ATTAAGTCAG GCGCGCCTCT AGTTGAAGAC 18420

ACGTTCATGT CTTCATCGTA AGAAGACACT CAGTAGTCTT CGGCCAGAAT GGCCATCTGG 184 80

ATTCAGCAGG CCTAGAAGGC CATTTAAATC CTGAGGATCT GGTCTTCCTA AGGACCCGGG 1854 0

ATATCGCTAT CAACTTTGTA TAGAAAAGTT GGGCCGAATT CGCCCTTGTT TAAACTTAAT 18 600

ATTTGTTTAA ACTTTTTACT AAATTCATGT AATAATTAAT GTATGCGTTA TATATATATG 18 660

TCTAGGTTTA TAATTATTCA TATGAATATG AACATAAAAA TCTAGGGCTA AAACGACTAC 18720

TATTTTGAAA ACGGAAGGAG TAGTAAGTTA TTTAAGCGGA GGGGAACCAT GATGGGCTAG 18780

TGATTTAATT TACATATATA TATTGGTGTT CTGGGCTCTT ACATGAGAAG ATCTAGTTAA 1884 0

CTGTTGTTAC TGAACAGCGA AGACAAATAT ATAATTTAAG CTCCCCAACT GCTAGTGATT 18 900

CTGTTAAGAG GTAATGTTTA AAGTAAATTT ACAAGAGCCC GTCTAGCTCA GTCGGTAGAG 18960

CGCAAGGCTC TTAACCTTGT GGTCGTGGGT TCGAGCCCCA CGGTGGGCGC ACAATTTTTT 19020

GTTTTTTGAC ATTTTTTGTT TGCTTAGTTG CAGACGGTTT TTCCCCTGCT AGGAGATTTC 19080

CGAGAGAAAA AAAAGGCACT ACAGGTTAAC CAAAACCACC AACCTTTGGA GCGTCGAGGC 1914 0

GACGGGGCAT TTGCGTAGTT GAAGCTTACA AAGTTGCATA TGAGATGAGT GCCGGACATG 19200

AAGCGGATAA CGTTTTAAAC TGGCAACAAT ATCTAGCTGT TTCAAATTCA GGCGTGGGAA 192 60

GCTACGCCTA CGCGCCCTGG ACGGCGTGTA AAGAGCCAGC ATCGGCATCA TTGTCAAACG 19320

ATCGACAAGG CCAAGAAATT CCAAATATAT TATTAATAAA AAAGAAGGCA CCAAATTAGT 19380

TTTTGTTTTT TAGTATGTGT GGCGGAGGAA ATTTTGAGAA CGAACGTATC CAAAGAAGGC 19440

ACAAGACGAT ATAGATTGAC GCGGCTAGAA AGTTGCAGCA AGACAGTGGG TACGGTCTTA 19500

TATATCCTAA TAAATAAAAA ATAAAACTAT AGTGTGTCAA ATGTCAACAA GAGGAGGAGG 19560

CAGCCAAATT AGCAGAGGGA GACAAGTAGA GCACGCCTTA TTAGCTTGCT TATTTATCGT 19620

GGTGGTGTAC TTGTTAATTA CTGGCACGCA TTATCAACAA CGCAGTTCTG GATGTGAATC 19680

TAGACAAACA TTTGTCTAGG TTCCGCACGT ATAGTTTTTT TTCTTTTTTT TTGGGGGGGG 19740

GGGGGAACGG AAGCTGTAAT AAACGGTACT AGGAACGAAA GCAACCGCCG CGCGCATGTT 19800

TTTGCAATAG ATTACGGTGA CCTTGATGCA CCACCGCGTG CTATAAAAAC CAGTGTCCCC 198 60

GAGTCTACTC ATCAACCAAT CCATAACTCG AAACCTTTTC TTGTGCTCTG TTCTGTCTGT 19920

GTGTTTCCAA AGCAAGCGAA AGAGGTCGAG GGGATCAGCT TCAAGTTTGT ACAAAAAAGC 19980

AGGCTCCGCG GCCGCCCCCT TCACCATGGC TCGGCAGCAA AGCGTGCAGG CCTTGTGTGT 2004 0

GCTGGCGGCG CTTCTCTTCG CCGCCTCCCT GCCGTCGCCG GCCGCCGCGG GGGTGCACCT 20100

CTCCTCGCTG CCCAAAGCGC TCGACGTCAC CACCTCCGCC AAACCCGGCC AAGTCCTGCA 20160

CGCCGGCGTG GACTCGCTGA CGGTGACGTG GAGCCTGAAC GCCACGGAGC CGGCCGGCGC 20220

CGACGCCGGG TACAAGGGCG TGAAGGTGAA GCTGTGCTAC GCGCCGGCGA GCCAGAAGGA 20280

CCGCGGGTGG CGCAAGTCCG AGGACGACAT CAGCAAGGAC AAGGCGTGCC AGTTCAAGGT 20340

CACCGAGCAG GCGTACGCGG CGGCGGCGCC CGGCAGCTTC CAGTACGCCG TCGCCCGCGA 204 00

CGTCCCCTCG GGCTCCTACT ACCTGCGCGC CTTCGCCACG GACGCGTCGG GCGCCGAGGT 204 60

GGCCTACGGC CAGACGGCGC CCACCGCCGC CTTCGACGTC GCCGGCATCA CCGGCATCCA 20520

CGCCTCTCTC AAGATCGCCG CCGGCGTCTT CTCGGCCTTC TCCGTCGTCG CGCTCGCCTT 20580

CTTCTTCGTC ATCGAGACCC GCAAGAAGAA CAAGTAGAAG GGTGGGCGCG CCGACCCAGC 20640

TTTCTTGTAC AAAGTGGCCG TTAACGGATC CAGACTTGTC CATCTTCTGG ATTGGCCAAC 20700

TTAATTAATG TATGAAATAA AAGGATGCAC ACATAGTGAC ATGCTAATCA CTATAATGTG 207 60

GGCATCAAAG TTGTGTGTTA TGTGTAATTA CTAGTTATCT GAATAAAAGA GAAAGAGATC 20820

ATCCATATTT CTTATCCTAA ATGAATGTCA CGTGTCTTTA TAATTCTTTG ATGAACCAGA 20880

TGCATTTCAT TAACCAAATC CATATACATA TAAATATTAA TCATATATAA TTAATATCAA 20940

TTGGGTTAGC AAAACAAATC TAGTCTAGGT GTGTTTTGCG AATTGCGGCA AGCTTGCGGC 21000

CGCCCCGGGC AACTTTATTA TACAAAGTTG ATAGATATCG GACCGATTAA ACTTTAATTC 21060

GGTCCGAAGC TTGCATGCCT GCAGTGCAGC GTGACCCGGT CGTGCCCCTC TCTAGAGATA 21120

ATGAGCATTG CATGTCTAAG TTATAAAAAA TTACCACATA TTTTTTTTGT CACACTTGTT 21180

TGAAGTGCAG TTTATCTATC TTTATACATA TATTTAAACT TTACTCTACG AATAATATAA 21240

TCTATAGTAC TACAATAATA TCAGTGTTTT AGAGAATCAT ATAAATGAAC AGTTAGACAT 21300

GGTCTAAAGG ACAATTGAGT ATTTTGACAA CAGGACTCTA CAGTTTTATC TTTTTAGTGT 21360

GCATGTGTTC TCCTTTTTTT TTGCAAATAG CTTCACCTAT ATAATACTTC ATCCATTTTA 21420

TTAGTACATC CATTTAGGGT TTAGGGTTAA TGGTTTTTAT AGACTAATTT TTTTAGTACA 21480

TCTATTTTAT TCTATTTTAG CCTCTAAATT AAGAAAACTA AAACTCTATT TTAGTTTTTT 21540

TATTTAATAA TTTAGATATA AAATAGAATA AAATAAAGTG ACTAAAAATT AAACAAATAC 21600

CCTTTAAGAA ATTAAAAAAA CTAAGGAAAC ATTTTTCTTG TTTCGAGTAG ATAATGCCAG 21660

CCTGTTAAAC GCCGTCGACG AGTCTAACGG ACACCAACCA GCGAACCAGC AGCGTCGCGT 21720

CGGGCCAAGC GAAGCAGACG GCACGGCATC TCTGTCGCTG CCTCTGGACC CCTCTCGAGA 21780GTTCCGCTCC ACCGTTGGAC TTGCTCCGCT GTCGGCATCC AGAAATTGCG TGGCGGAGCG 2184 0

GCAGACGTGA GCCGGCACGG CAGGCGGCCT CCTCCTCCTC TCACGGCACC GGCAGCTACG 21900

GGGGATTCCT TTCCCACCGC TCCTTCGCTT TCCCTTCCTC GCCCGCCGTA ATAAATAGAC 21960

ACCCCCTCCA CACCCTCTTT CCCCAACCTC GTGTTGTTCG GAGCGCACAC ACACACAACC 22020

AGATCTCCCC CAAATCCACC CGTCGGCACC TCCGCTTCAA GGTACGCCGC TCGTCCTCCC 22080

CCCCCCCCCT CTCTACCTTC TCTAGATCGG CGTTCCGGTC CATGCATGGT TAGGGCCCGG 22140

TAGTTCTACT TCTGTTCATG TTTGTGTTAG ATCCGTGTTT GTGTTAGATC CGTGCTGCTA 22200

GCGTTCGTAC ACGGATGCGA CCTGTACGTC AGACACGTTC TGATTGCTAA CTTGCCAGTG 22260

TTTCTCTTTG GGGAATCCTG GGATGGCTCT AGCCGTTCCG CAGACGGGAT CGATTTCATG 22320

ATTTTTTTTG TTTCGTTGCA TAGGGTTTGG TTTGCCCTTT TCCTTTATTT CAATATATGC 22380

CGTGCACTTG TTTGTCGGGT CATCTTTTCA TGCTTTTTTT TGTCTTGGTT GTGATGATGT 22440

GGTCTGGTTG GGCGGTCGTT CTAGATCGGA GTAGAATTCT GTTTCAAACT ACCTGGTGGA 22500

TTTATTAATT TTGGATCTGT ATGTGTGTGC CATACATATT CATAGTTACG AATTGAAGAT 22560

GATGGATGGA AATATCGATC TAGGATAGGT ATACATGTTG ATGCGGGTTT TACTGATGCA 22620

TATACAGAGA TGCTTTTTGT TCGCTTGGTT GTGATGATGT GGTGTGGTTG GGCGGTCGTT 22 680

CATTCGTTCT AGATCGGAGT AGAATACTGT TTCAAACTAC CTGGTGTATT TATTAATTTT 22740

GGAACTGTAT GTGTGTGTCA TACATCTTCA TAGTTACGAG TTTAAGATGG ATGGAAATAT 22800

CGATCTAGGA TAGGTATACA TGTTGATGTG GGTTTTACTG ATGCATATAC ATGATGGCAT 22860

ATGCAGCATC TATTCATATG CTCTAACCTT GAGTACCTAT CTATTATAAT AAACAAGTAT 22 920

GTTTTATAAT TATTTTGATC TTGATATACT TGGATGATGG CATATGCAGC AGCTATATGT 22 980

GGATTTTTTT AGCCCTGCCT TCATACGCTA TTTATTTGCT TGGTACTGTT TCTTTTGTCG 23040

ATGCTCACCC TGTTGTTTGG TGTTACTTCT GCAGGTCGAC TTTAACTTAG CCTAGGATCC 23100

ACACGACACC ATGTCCCCCG AGCGCCGCCC CGTCGAGATC CGCCCGGCCA CCGCCGCCGA 23160

CATGGCCGCC GTGTGCGACA TCGTGAACCA CTACATCGAG ACCTCCACCG TGAACTTCCG 23220

CACCGAGCCG CAGACCCCGC AGGAGTGGAT CGACGACCTG GAGCGCCTCC AGGACCGCTA 23280

CCCGTGGCTC GTGGCCGAGG TGGAGGGCGT GGTGGCCGGC ATCGCCTACG CCGGCCCGTG 23340

GAAGGCCCGC AACGCCTACG ACTGGACCGT GGAGTCCACC GTGTACGTGT CCCACCGCCA 234 00

CCAGCGCCTC GGCCTCGGCT CCACCCTCTA CACCCACCTC CTCAAGAGCA TGGAGGCCCA 234 60

GGGCTTCAAG TCCGTGGTGG CCGTGATCGG CCTCCCGAAC GACCCGTCCG TGCGCCTCCA 23520

CGAGGCCCTC GGCTACACCG CCCGCGGCAC CCTCCGCGCC GCCGGCTACA AGCACGGCGG 23580

CTGGCACGAC GTCGGCTTCT GGCAGCGCGA CTTCGAGCTG CCGGCCCCGC CGCGCCCGGT 23640

GCGCCCGGTG ACGCAGATCT GAGTCGAAAC CTAGACTTGT CCATCTTCTG GATTGGCCAA 23700

CTTAATTAAT GTATGAAATA AAAGGATGCA CACATAGTGA CATGCTAATC ACTATAATGT 237 60

GGGCATCAAA GTTGTGTGTT ATGTGTAATT ACTAGTTATC TGAATAAAAG AGAAAGAGAT 23820

CATCCATATT TCTTATCCTA AATGAATGTC ACGTGTCTTT ATAATTCTTT GATGAACCAG 23880

ATGCATTTCA TTAACCAAAT CCATATACAT ATAAATATTA ATCATATATA ATTAATATCA 23940

ATTGGGTTAG CAAAACAAAT CTAGTCTAGG TGTGTTTTGC GAATGCGGCC GCCACCGCGG 24 000

TGGAGCTCGA ATTCATTCCG ATTAATCGTG GCCTCTTGCT CTTCAGGATG AAGAGCTATG 24 060

TTTAAACGTG CAAGCGCTAC TAGACAATTC AGTACATTAA AAACGTCCGC AATGTGTTAT 24120

TAAGTTGTCT AAGCGTCAAT TTGTTTACAC CACAATATAT CCTGCCACCA GCCAGCCAAC 24180

AGCTCCCCGA CCGGCAGCTC GGCACAAAAT CACCACTCGA TACAGGCAGC CCATCAGTCC 24240

GGGACGGCGT CAGCGGGAGA GCCGTTGTAA GGCGGCAGAC TTTGCTCATG TTACCGATGC 24300

TATTCGGAAG AACGGCAACT AAGCTGCCGG GTTTGAAACA CGGATGATCT CGCGGAGGGT 24360

AGCATGTTGA TTGTAACGAT GACAGAGCGT TGCTGCCTGT GATCAAATAT CATCTCCCTC 24 420

GCAGAGATCC GAATTATCAG CCTTCTTATT CATTTCTCGC TTAACCGTGA CAGGCTGTCG 24 480

ATCTTGAGAA CTATGCCGAC ATAATAGGAA ATCGCTGGAT AAAGCCGCTG AGGAAGCTGA 24 540

GTGGCGCTAT TTCTTTAGAA GTGAACGTTG ACGATCGTCG ACCGTACCCC GATGAATTAA 24 600

TTCGGACGTA CGTTCTGAAC ACAGCTGGAT ACTTACTTGG GCGATTGTCA TACATGACAT 24 660

CAACAATGTA CCCGTTTGTG TAACCGTCTC TTGGAGGTTC GTATGACACT AGTGGTTCCC 24720

CTCAGCTTGC GACTAGATGT TGAGGCCTAA CATTTTATTA GAGAGCAGGC TAGTTGCTTA 24780

GATACATGAT CTTCAGGCCG TTATCTGTCA GGGCAAGCGA AAATTGGCCA TTTATGACGA 2484 0

CCAATGCCCC GCAGAAGCTC CCATCTTTGC CGCCATAGAC GCCGCGCCCC CCTTTTGGGG 24 900

TGTAGAACAT CCTTTTGCCA GATGTGGAAA AGAAGTTCGT TGTCCCATTG TTGGCAATGA 24 960

CGTAGTAGCC GGCGAAAGTG CGAGACCCAT TTGCGCTATA TATAAGCCTA CGATTTCCGT 25020

TGCGACTATT GTCGTAATTG GATGAACTAT TATCGTAGTT GCTCTCAGAG TTGTCGTAAT 25080

TTGATGGACT ATTGTCGTAA TTGCTTATGG AGTTGTCGTA GTTGCTTGGA GAAATGTCGT 25140

AGTTGGATGG GGAGTAGTCA TAGGGAAGAC GAGCTTCATC CACTAAAACA ATTGGCAGGT 25200

CAGCAAGTGC CTGCCCCGAT GCCATCGCAA GTACGAGGCT TAGAACCACC TTCAACAGAT 25260

CGCGCATAGT CTTCCCCAGC TCTCTAACGC TTGAGTTAAG CCGCGCCGCG AAGCGGCGTC 25320GGCTTGAACG AATTGTTAGA CATTATTTGC CGACTACCTT GGTGATCTCG CCTTTCACGT 25380

AGTGAACAAA TTCTTCCAAC TGATCTGCGC GCGAGGCCAA GCGATCTTCT TGTCCAAGAT 254 40

AAGCCTGCCT AGCTTCAAGT ATGACGGGCT GATACTGGGC CGGCAGGCGC TCCATTGCCC 25500

AGTCGGCAGC GACATCCTTC GGCGCGATTT TGCCGGTTAC TGCGCTGTAC CAAATGCGGG 25560

ACAACGTAAG CACTACATTT CGCTCATCGC CAGCCCAGTC GGGCGGCGAG TTCCATAGCG 25620

TTAAGGTTTC ATTTAGCGCC TCAAATAGAT CCTGTTCAGG AACCGGATCA AAGAGTTCCT 25680

CCGCCGCTGG ACCTACCAAG GCAACGCTAT GTTCTCTTGC TTTTGTCAGC AAGATAGCCA 25740

GATCAATGTC GATCGTGGCT GGCTCGAAGA TACCTGCAAG AATGTCATTG CGCTGCCATT 25800

CTCCAAATTG CAGTTCGCGC TTAGCTGGAT AACGCCACGG AATGATGTCG TCGTGCACAA 25860

CAATGGTGAC TTCTACAGCG CGGAGAATCT CGCTCTCTCC AGGGGAAGCC GAAGTTTCCA 25920

AAAGGTCGTT GATCAAAGCT CGCCGCGTTG TTTCATCAAG CCTTACAGTC ACCGTAACCA 25980

GCAAATCAAT ATCACTGTGT GGCTTCAGGC CGCCATCCAC TGCGGAGCCG TACAAATGTA 26040

CGGCCAGCAA CGTCGGTTCG AGATGGCGCT CGATGACGCC AACTACCTCT GATAGTTGAG 2 6100

TCGATACTTC GGCGATCACC GCTTCCCTCA TGATGTTTAA CTCCTGAATT AAGCCGCGCC 26160

GCGAAGCGGT GTCGGCTTGA ATGAATTGTT AGGCGTCATC CTGTGCTCCC GAGAACCAGT 26220

ACCAGTACAT CGCTGTTTCG TTCGAGACTT GAGGTCTAGT TTTATACGTG AACAGGTCAA 26280

TGCCGCCGAG AGTAAAGCCA CATTTTGCGT ACAAATTGCA GGCAGGTACA TTGTTCGTTT 26340

GTGTCTCTAA TCGTATGCCA AGGAGCTGTC TGCTTAGTGC CCACTTTTTC GCAAATTCGA 264 00

TGAGACTGTG CGCGACTCCT TTGCCTCGGT GCGTGTGCGA CACAACAATG TGTTCGATAG 264 60

AGGCTAGATC GTTCCATGTT GAGTTGAGTT CAATCTTCCC GACAAGCTCT TGGTCGATGA 26520

ATGCGCCATA GCAAGCAGAG TCTTCATCAG AGTCATCATC CGAGATGTAA TCCTTCCGGT 26580

AGGGGCTCAC ACTTCTGGTA GATAGTTCAA AGCCTTGGTC GGATAGGTGC ACATCGAACA 26640

CTTCACGAAC AATGAAATGG TTCTCAGCAT CCAATGTTTC CGCCACCTGC TCAGGGATCA 26700

CCGAAATCTT CATATGACGC CTAACGCCTG GCACAGCGGA TCGCAAACCT GGCGCGGCTT 267 60

TTGGCACAAA AGGCGTGACA GGTTTGCGAA TCCGTTGCTG CCACTTGTTA ACCCTTTTGC 26820

CAGATTTGGT AACTATAATT TATGTTAGAG GCGAAGTCTT GGGTAAAAAC TGGCCTAAAA 26880

TTGCTGGGGA TTTCAGGAAA GTAAACATCA CCTTCCGGCT CGATGTCTAT TGTAGATATA 2694 0

TGTAGTGTAT CTACTTGATC GGGGGATCTG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA 27000

AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 27060

GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT 27120

GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG 27180

ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA 2724 0

TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 27300

CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 27360

GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 27 420

GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 27480

CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 27 54 0

GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 27 600

TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 27 660

GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 27 720

TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 27780

CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 2784 0

TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 27 900

CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 27960

ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 28020

TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 28080

CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 28140

TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 28200

CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 28260

GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 28320

GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 28380

CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 28440

ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 28500

GTTGCCATTG CTGCAGGGGG GGGGGGGGGG GGGGACTTCC ATTGTTCATT CCACGGACAA 28560

AAACAGAGAA AGGAAACGAC AGAGGCCAAA AAGCCTCGCT TTCAGCACCT GTCGTTTCCT 28 620

TfICTTTTCAG AGGGTATTTT AAATAAAAAC ATTAAGTTAT GACGAAGAAG AACGGAAACG 28 680

CCTTAAACCG GAAAATTTTC ATAAATAGCG AAAACCCGCG AGGTCGCCGC CCCGTAAGCC 28740

GCCCCGTAAC CTGTCGGATC ACCGGAAAGG ACCCGTAAAG TGATAATGAT TATCATCTAC 28800

ATATCACAAC GTGCGTGGAG GCCATCAAAC CACGTCAAAT AATCAATTAT GACGCAGGTA 28860TCGTATTAAT TGATCTGCAT CAACTTAACG TAAAAACAAC TTCAGACAAT ACAAATCAGC 28920

GACACTGAAT ACGGGGCAAC CTCATGTCCC CCCCCCCCCC CCCCCTGCAG GCATCGTGGT 28980

GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 29040

TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 2 9100

CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 29160

TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 29220

CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC 29280

CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 2 9340

ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 29400

CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 294 60

AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 29520

TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 29580

ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 2964 0

TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 29700

GCCCTTTCGT CTTCAAGAAT TCGGAGCTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC 29760

ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG 2 9820

ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC 29880

TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC 29940

CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG 30000

TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GGCTTTGTTG 30060

AATAAATCGA ACTTTTGCTG AGTTGAAGGA TCAGATCACG CATCTTCCCG ACAACGCAGA 30120

CCGTTCCGTG GCAAAGCAAA AGTTCAAAAT CACCAACTGG TCCACCTACA ACAAAGCTCT 30180

CATCAACCGT GGCTCCCTCA CTTTCTGGCT GGATGATGGG GCGATTCAGG CCTGGTATGA 3024 0

GTCAGCAACA CCTTCTTCAC GAGGCAGACC TCAGCGCCAG AAGGCCGCCA GAGAGGCCGA 30300

GCGCGGCCGT GAGGCTTGGA CGCTAGGGCA GGGCATGAAA AAGCCCGTAG CGGGCTGCTA 30360

CGGGCGTCTG ACGCGGTGGA AAGGGGGAGG GGATGTTGTC TACATGGCTC TGCTGTAGTG 304 20

AGTGGGTTGC GCTCCGGCAG CGGTCCTGAT CAATCGTCAC CCTTTCTCGG TCCTTCAACG 304 80

TTCCTGACAA CGAGCCTCCT TTTCGCCAAT CCATCGACAA TCACCGCGAG TCCCTGCTCG 3054 0

AACGCTGCGT CCGGACCGGC TTCGTCGAAG GCGTCTATCG CGGCCCGCAA CAGCGGCGAG 30600

AGCGGAGCCT GTTCAACGGT GCCGCCGCGC TCGCCGGCAT CGCTGTCGCC GGCCTGCTCC 30660

TCAAGCACGG CCCCAACAGT GAAGTAGCTG ATTGTCATCA GCGCATTGAC GGCGTCCCCG 30720

GCCGAAAAAC CCGCCTCGCA GAGGAAGCGA AGCTGCGCGT CGGCCGTTTC CATCTGCGGT 30780

GCGCCCGGTC GCGTGCCGGC ATGGATGCGC GCGCCATCGC GGTAGGCGAG CAGCGCCTGC 3084 0

CTGAAGCTGC GGGCATTCCC GATCAGAAAT GAGCGCCAGT CGTCGTCGGC TCTCGGCACC 30900

GAATGCGTAT GATTCTCCGC CAGCATGGCT TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG 30960

TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC TGAACCCCCA ACCGTTCCGC CAGTTTGCGT 31020

GTCGTCAGAC CGTCTACGCC GACCTCGTTC AACAGGTCCA GGGCGGCACG GATCACTGTA 31080

TTCGGCTGCA ACTTTGTCAT GCTTGACACT TTATCACTGA TAAACATAAT ATGTCCACCA 3114 0

ACTTATCAGT GATAAAGAAT CCGCGCGTTC AATCGGACCA GCGGAGGCTG GTCCGGAGGC 31200

CAGACGTGAA ACCCAACATA CCCCTGATCG TAATTCTGAG CACTGTCGCG CTCGACGCTG 31260

TCGGCATCGG CCTGATTATG CCGGTGCTGC CGGGCCTCCT GCGCGATCTG GTTCACTCGA 31320

ACGACGTCAC CGCCCACTAT GGCATTCTGC TGGCGCTGTA TGCGTTGGTG CAATTTGCCT 31380

GCGCACCTGT GCTGGGCGCG CTGTCGGATC GTTTCGGGCG GCGGCCAATC TTGCTCGTCT 314 40

CGCTGGCCGG CGCCACTGTC GACTACGCCA TCATGGCGAC AGCGCCTTTC CTTTGGGTTC 31500

TCTATATCGG GCGGATCGTG GCCGGCATCA CCGGGGCGAC TGGGGCGGTA GCCGGCGCTT 31560

ATATTGCCGA TATCACTGAT GGCGATGAGC GCGCGCGGCA CTTCGGCTTC ATGAGCGCCT 31620

GTTTCGGGTT CGGGATGGTC GCGGGACCTG TGCTCGGTGG GCTGATGGGC GGTTTCTCCC 31680

CCCACGCTCC GTTCTTCGCC GCGGCAGCCT TGAACGGCCT CAATTTCCTG ACGGGCTGTT 31740

TCCTTTTGCC GGAGTCGCAC AAAGGCGAAC GCCGGCCGTT ACGCCGGGAG GCTCTCAACC 31800

CGCTCGCTTC GTTCCGGTGG GCCCGGGGCA TGACCGTCGT CGCCGCCCTG ATGGCGGTCT 318 60

TCTTCATCAT GCAACTTGTC GGACAGGTGC CGGCCGCGCT TTGGGTCATT TTCGGCGAGG 31920

ATCGCTTTCA CTGGGACGCG ACCACGATCG GCATTTCGCT TGCCGCATTT GGCATTCTGC 31980

ATTCACTCGC CCAGGCAATG ATCACCGGCC CTGTAGCCGC CCGGCTCGGC GAAAGGCGGG 32040

CACTCATGCT CGGAATGATT GCCGACGGCA CAGGCTACAT CCTGCTTGCC TTCGCGACAC 32100

GGGGATGGAT GGCGTTCCCG ATCATGGTCC TGCTTGCTTC GGGTGGCATC GGAATGCCGG 32160

CGCTGCAAGC AATGTTGTCC AGGCAGGTGG ATGAGGAACG TCAGGGGCAG CTGCAAGGCT 32220

CACTGGCGGC GCTCACCAGC CTGACCTCGA TCGTCGGACC CCTCCTCTTC ACGGCGATCT 32280

ATGCGGCTTC TATAACAACG TGGAACGGGT GGGCATGGAT TGCAGGCGCT GCCCTCTACT 3234 0

TGCTCTGCCT GCCGGCGCTG CGTCGCGGGC TTTGGAGCGG CGCAGGGCAA CGAGCCGATC 32400gctgatcgtg gaaacgatag gcctatgccaacgcgcccta ggagtgcggt tggaacgttggacgccgatg atttgaagcg cactcagcgtggcggtcccc gggctgagaa agcccagtaacccccggaac caaaggaagt aggttaaaccgagaacattg gttcctgtag gcatcgggatgagcagaagt cctccggccg ccagttgccattgccacttg cgggtcagca cggttccgaatgcgacgccg acaggatcta gcgctgcgttagttccgcaa atagccccca ggaccgccatagagatgaac acgaccatca gcggctgcaccaggcggtag accgaaataa acaacaagctgatgaagatg cgcatccacc agattcccgttaaacccgcc ggcaacgccc gcagcagcatctccacgaaa acgccggaca gatgcgccttgaagaccgac agcccaatga tctcgccgtccaaccgttca gcgaacgcct ccatgggcttcatctctgga gctttcttca gggccgacaacgctccaagc cgtcgaatct gagccttaatcggcagttcg tagagcgcgc cgtgcgtcccgcagtgcccg cttgttcctg aaatgccagtactgacccca caaggcccta gcgtttgcaatccaccaggc cgctgcctcg caactcttcgttcacgcggg tggaatccga tccgcacatgggctcccggt gcgagctgaa atagtcgaactacttctccc atatgaattt cgtgtagtggtcgatcagga cctggcaacg ggacgttttcagcagcgaca ccgattccag gtgcccaacgtgtaggcgcg acaggcattc ctcggccttcaggtcctggc aaagctcgta gaacgtgaaggcgtactcca acacctgctg ccacaccagtgtgtaggtga tcttcacgtc cttgttgacgcgcgggattt tcttgttgcg cgtggtgaacgctcgcatcg tgtccggcca cggcgcaatatgctgcttcg tgtgtttcag caacgcggcctcctcgccgg cggtttttcg cttcttggtcgacttcgcca aacctgccgc ctcctgttcgggcgcgggca gggcaggggg agccagttgctgctggacca tcgagccgac ggactggaagcttgcgatgg tttcggcatc ctcggcggaagccttccggt caaacgtccg attcattcacggggcaatgt gcccttattc ctgatttgacaccttatcgg caatgaagtc ggtcccgtagttccgaatct tgccctgcac gaataccagctcggcctgag agccaaaaca cttgatgcgggcatcgttgc gccactcttc attaaccgctttgccatgac gtacctcggt gtcacgggtactcatatcga aagtctcctt gagaaaggaggtcaagaact ttagcggcta aaattttgcgccgctgtgta caaccagata tttttcaccatgacgaaaca tgagctgtcg gagagggcagccaacggtaa ggccaacccc tcgttgaaggctcccctacc tcttctcctg gagtccaccgcgggtcatcc tttcaagagc agcgtgccgccgtcacataa ggcgtttatc gtaaagaaatgaaggctctg acgccaaggg ttagggcttgcttctctgcg ggccgtcggc tcgcgcatcagaatggcatc gggcgggtgc gctttgacagggttcgatta gctgtttgtc ttgcaggcta

tgcgggtcaa ggcgacttcc ggcaagctat 324 60

gcccagccag atactcccga tcacgagcag 32520

ctgatccaag aacaaccatc ctagcaacac 32580

ggaaacaact gtaggttcga gtcgcgagat 3264 0

cgctccgatc aggccgagcc acgccaggcc 32700

tggcggatca aacactaaag ctactggaac 32760

ggcggtaaag gtgagcagag gcacgggagg 32820

cgccatggaa accgcccccg ccaggcccgc 32880

tggtgtcaac accaacagcg ccacgcccgc 3294 0

caatcgtatc gggctaccta gcagagcggc 33000

agcgcctacc gtcgccgcga ccccgcccgg 33060

ccagaatagc gaaatattaa gtgcgccgag 33120

tggaatctgt cggacgatca tcacgagcaa 33180

accggcgacc cctcggcctc gctgttcggg 33240

gtgagcgtcc ttggggccgt cctcctgttt 33300

gatgtaggcg ccgaatgcca cggcatctcg 33360

tttctcctcg tgctcgtaaa cggacccgaa 334 20

tcggatctcg cggaaatcct gcacgtcggc 334 80

cacaattgtc aattttaatc ctctgtttat 33540

gagcgatact gagcgaagca agtgcgtcga 33600

aaagcgctgg ctgctgaacc cccagccgga 33660

tgcaccaggt catcattgac ccaggcgtgt 337 20

caggcttcgc cgacctgctc gcgccacttc 33780

aggcggaagg tttccagctt gagcgggtac 3384 0

atccgtcggg ccgtcggcga cagcttgcgg 33900

tcgccagcaa acagcacgac gatttcctcg 33960

ttgccacggt ccaggacgcg gaagcggtgc 34 020

cggtcggacg tgaagcccat cgccgtcgcc 34 080

gtgtaatacc ggccattgat cgaccagccc 34140

gtgatcggct cgccgatagg ggtgcgcttc 34200

tcgtcatcgt cggcccgcag ctcgacgccg 34 260

tggaaaatga ccttgttttg cagcgcctcg 34 320

agggcagagc gggccgtgtc gtttggcatc 34 380

tcgaacaagg aaagctgcat ttccttgatc 34 4 40

tgcttggcct cgctgacctg ttttgccagg 34 500

gtcatagttc ctcgcgtgtc gatggtcatc 34560

agacgacgcg aacgctccac ggcggccgat 34 620

acgctgtcgc gctcgatctt ggccgtagct 34 680

gtttcgcggg gcgcacgcat gacggtgcgg 34740

aaccccgcgt cgatcagttc ttgcctgtat 34 800

cctccttgcg ggattgcccc gactcacgcc 34 8 60

ccgcctggtg ccttggtgtc cagataatcc 34 920

accgtctggc cgtccttctc gtacttggta 34 980

gaccccttgc ccaaatactt gccgtgggcc 35040

aagaagtcgg tgcgctcctg cttgtcgccg 35100

atatcgaaaa ttgcttgcgg cttgttagaa 35160

agattaccga taaactggaa ctgattatgg 35220

actctagttt agctaaacat tggttccgct 35280

ggccgcgacc aaaggtgcga ggggcggctt 35340

acatccttcg tctgctcgat gagcggggca 354 00gggtttcaat ttcgttttta tcagacttaa 354 60tgatggaggc cattgccgac gccctggaaa 35520

accttgaccg cgaggcactc gcggagattg 35580

ccggatacga acgcatcagt gtggttttgc 35640

ggggcgacga cacccgaaaa aagctgcgtg 35700

cacttccttc tttagccgct aaaacggccc 357 60

tatcgacatc ctcaacggaa gccgtgccgc 35820

ttgttttcta tcagaacccc tacgtcgtgc 35880

aacactttcg gtatatcgtt tgcctgtgcg 35940ATAATGTTGC TAATGATTTG TTGCGTAGGG GTTACTGAAA AGTGAGCGGG AAAGAAGAGT 36000

TTCAGACCAT CAAGGAGCGG GCCAAGCGCA AGCTGGAACG CGACATGGGT GCGGACCTGT 36060

TGGCCGCGCT CAACGACCCG AAAACCGTTG AAGTCATGCT CAACGCGGAC GGCAAGGTGT 36120

GGCACGAACG CCTTGGCGAG CCGATGCGGT ACATCTGCGA CATGCGGCCC AGCCAGTCGC 36180

AGGCGATTAT AGAAACGGTG GCCGGATTCC ACGGCAAAGA GGTCACGCGG CATTCGCCCA 36240

TCCTGGAAGG CGAGTTCCCC TTGGATGGCA GCCGCTTTGC CGGCCAATTG CCGCCGGTCG 36300

TGGCCGCGCC AACCTTTGCG ATCCGCAAGC GCGCGGTCGC CATCTTCACG CTGGAACAGT 36360

ACGTCGAGGC GGGCATCATG ACCCGCGAGC AATACGAGGT CATTAAAAGC GCCGTCGCGG 364 20

CGCATCGAAA CATCCTCGTC ATTGGCGGTA CTGGCTCGGG CAAGACCACG CTCGTCAACG 36480

CGATCATCAA TGAAATGGTC GCCTTCAACC CGTCTGAGCG CGTCGTCATC ATCGAGGACA 36540

CCGGCGAAAT CCAGTGCGCC GCAGAGAACG CCGTCCAATA CCACACCAGC ATCGACGTCT 36600

CGATGACGCT GCTGCTCAAG ACAACGCTGC GTATGCGCCC CGACCGCATC CTGGTCGGTG 36660

AGGTACGTGG CCCCGAAGCC CTTGATCTGT TGATGGCCTG GAACACCGGG CATGAAGGAG 36720

GTGCCGCCAC CCTGCACGCA AACAACCCCA AAGCGGGCCT GAGCCGGCTC GCCATGCTTA 36780

TCAGCATGCA CCCGGATTCA CCGAAACCCA TTGAGCCGCT GATTGGCGAG GCGGTTCATG 36840

TGGTCGTCCA TATCGCCAGG ACCCCTAGCG GCCGTCGAGT GCAAGAAATT CTCGAAGTTC 36900

TTGGTTACGA GAACGGCCAG TACATCACCA AAACCCTGTA AGGAGTATTT CCAATGACAA 36960

CGGCTGTTCC GTTCCGTCTG ACCATGAATC GCGGCATTTT GTTCTACCTT GCCGTGTTCT 37 020

TCGTTCTCGC TCTCGCGTTA TCCGCGCATC CGGCGATGGC CTCGGAAGGC ACCGGCGGCA 37080

GCTTGCCATA TGAGAGCTGG CTGACGAACC TGCGCAACTC CGTAACCGGC CCGGTGGCCT 3714 0

TCGCGCTGTC CATCATCGGC ATCGTCGTCG CCGGCGGCGT GCTGATCTTC GGCGGCGAAC 37200

TCAACGCCTT CTTCCGAACC CTGATCTTCC TGGTTCTGGT GATGGCGCTG CTGGTCGGCG 37260

CGCAGAACGT GATGAGCACC TTCTTCGGTC GTGGTGCCGA AATCGCGGCC CTCGGCAACG 37320

GGGCGCTGCA CCAGGTGCAA GTCGCGGCGG CGGATGCCGT GCGTGCGGTA GCGGCTGGAC 37 380

GGCTCGCCTA ATCATGGCTC TGCGCACGAT CCCCATCCGT CGCGCAGGCA ACCGAGAAAA 374 40

CCTGTTCATG GGTGGTGATC GTGAACTGGT GATGTTCTCG GGCCTGATGG CGTTTGCGCT 37500

GATTTTCAGC GCCCAAGAGC TGCGGGCCAC CGTGGTCGGT CTGATCCTGT GGTTCGGGGC 37 560

GCTCTATGCG TTCCGAATCA TGGCGAAGGC CGATCCGAAG ATGCGGTTCG TGTACCTGCG 37 620

TCACCGCCGG TACAAGCCGT ATTACCCGGC CCGCTCGACC CCGTTCCGCG AGAACACCAA 37680

TAGCCAAGGG AAGCAATACC GATGATCCAA GCAATTGCGA TTGCAATCGC GGGCCTCGGC 3774 0

GCGCTTCTGT TGTTCATCCT CTTTGCCCGC ATCCGCGCGG TCGATGCCGA ACTGAAACTG 37800

AAAAAGCATC GTTCCAAGGA CGCCGGCCTG GCCGATCTGC TCAACTACGC CGCTGTCGTC 37 8 60

GATGACGGCG TAATCGTGGG CAAGAACGGC AGCTTTATGG CTGCCTGGCT GTACAAGGGC 37920

GATGACAACG CAAGCAGCAC CGACCAGCAG CGCGAAGTAG TGTCCGCCCG CATCAACCAG 37 980

GCCCTCGCGG GCCTGGGAAG TGGGTGGATG ATCCATGTGG ACGCCGTGCG GCGTCCTGCT 38040

CCGAACTACG CGGAGCGGGG CCTGTCGGCG TTCCCTGACC GTCTGACGGC AGCGATTGAA 38100

GAAGAGCGCT CGGTCTTGCC TTGCTCGTCG GTGATGTACT TCACCAGCTC CGCGAAGTCG 38160

CTCTTCTTGA TGGAGCGCAT GGGGACGTGC TTGGCAATCA CGCGCACCCC CCGGCCGTTT 38220

TAGCGGCTAA AAAAGTCATG GCTCTGCCCT CGGGCGGACC ACGCCCATCA TGACCTTGCC 38280

AAGCTCGTCC TGCTTCTCTT CGATCTTCGC CAGCAGGGCG AGGATCGTGG CATCACCGAA 3834 0

CCGCGCCGTG CGCGGGTCGT CGGTGAGCCA GAGTTTCAGC AGGCCGCCCA GGCGGCCCAG 38400

GTCGCCATTG ATGCGGGCCA GCTCGCGGAC GTGCTCATAG TCCACGACGC CCGTGATTTT 384 60

GTAGCCCTGG CCGACGGCCA GCAGGTAGGC CGACAGGCTC ATGCCGGCCG CCGCCGCCTT 38520

TTCCTCAATC GCTCTTCGTT CGTCTGGAAG GCAGTACACC TTGATAGGTG GGCTGCCCTT 38580

CCTGGTTGGC TTGGTTTCAT CAGCCATCCG CTTGCCCTCA TCTGTTACGC CGGCGGTAGC 38 64 0

CGGCCAGCCT CGCAGAGCAG GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT 38700

GAAGAAGGAA CACCCGCTCG CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG 387 60

TTGGATACAC CAAGGAAAGT CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA 38820

AAAGGATGGA TATACCGAAA AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA 38880

AAAGCGCTGC TTCCCTGCTG TTTTGTGGAA TATCTACCGA CTGGAAACAG GCAAATGCAG 38940

GAAATTACTG AACTGAGGGG ACAGGCGAGA GACGATGCCA AAGAGCTACA CCGACGAGCT 39000

GGCCGAGTGG GTTGAATCCC GCGCGGCCAA GAAGCGCCGG CGTGATGAGG CTGCGGTTGC 39060

GTTCCTGGCG GTGAGGGCGG ATGTCGAGGC GGCGTTAGCG TCCGGCTATG CGCTCGTCAC 39120

CATTTGGGAG CACATGCGGG AAACGGGGAA GGTCAAGTTC TCCTACGAGA CGTTCCGCTC 39180

GCACGCCAGG CGGCACATCA AGGCCAAGCC CGCCGATGTG CCCGCACCGC AGGCCAAGGC 39240

TGCGGAACCC GCGCCGGCAC CCAAGACGCC GGAGCCACGG CGGCCGAAGC AGGGGGGCAA 39300

GGCTGAAAAG CCGGCCCCCG CTGCGGCCCC GACCGGCTTC ACCTTCAACC CAACACCGGA 39360

CAAAAAGGAT CTACTGTAAT GGCGAAAATT CACATGGTTT TGCAGGGCAA GGGCGGGGTC 39420

GGCAAGTCGG CCATCGCCGC GATCATTGCG CAGTACAAGA TGGACAAGGG GCAGACACCC 39480TTGTGCATCG ACACCGACCC GGTGAACGCG ACGTTCGAGG GCTACAAGGC CCTGAACGTC 39540

CGCCGGCTGA ACATCATGGC CGGCGACGAA ATTAACTCGC GCAACTTCGA CACCCTGGTC 39600

GAGCTGATTG CGCCGACCAA GGATGACGTG GTGATCGACA ACGGTGCCAG CTCGTTCGTG 39660

CCTCTGTCGC ATTACCTCAT CAGCAACCAG GTGCCGGCTC TGCTGCAAGA AATGGGGCAT 39720

GAGCTGGTCA TCCATACCGT CGTCACCGGC GGCCAGGCTC TCCTGGACAC GGTGAGCGGC 397 80

TTCGCCCAGC TCGCCAGCCA GTTCCCGGCC GAAGCGCTTT TCGTGGTCTG GCTGAACCCG 39840

TATTGGGGGC CTATCGAGCA TGAGGGCAAG AGCTTTGAGC AGATGAAGGC GTACACGGCC 39900

AACAAGGCCC GCGTGTCGTC CATCATCCAG ATTCCGGCCC TCAAGGAAGA AACCTACGGC 39960

CGCGATTTCA GCGACATGCT GCAAGAGCGG CTGACGTTCG ACCAGGCGCT GGCCGATGAA 4 0020

TCGCTCACGA TCATGACGCG GCAACGCCTC AAGATCGTGC GGCGCGGCCT GTTTGAACAG 4 0080

CTCGACGCGG CGGCCGTGCT ATGAGCGACC AGATTGAAGA GCTGATCCGG GAGATTGCGG 40140

CCAAGCACGG CATCGCCGTC GGCCGCGACG ACCCGGTGCT GATCCTGCAT ACCATCAACG 40200

CCCGGCTCAT GGCCGACAGT GCGGCCAAGC AAGAGGAAAT CCTTGCCGCG TTCAAGGAAG 4 0260

AGCTGGAAGG GATCGCCCAT CGTTGGGGCG AGGACGCCAA GGCCAAAGCG GAGCGGATGC 4 0320

TGAACGCGGC CCTGGCGGCC AGCAAGGACG CAATGGCGAA GGTAATGAAG GACAGCGCCG 4 0380

CGCAGGCGGC CGAAGCGATC CGCAGGGAAA TCGACGACGG CCTTGGCCGC CAGCTCGCGG 40440

CCAAGGTCGC GGACGCGCGG CGCGTGGCGA TGATGAACAT GATCGCCGGC GGCATGGTGT 4 0500

TGTTCGCGGC CGCCCTGGTG GTGTGGGCCT CGTTATGAAT CGCAGAGGCG CAGATGAAAA 40560

AGCCCGGCGT TGCCGGGCTT TGTTTTTGCG TTAGCTGGGC TTGTTTGACA GGCCCAAGCT 4 0620

CTGACTGCGC CCGCGCTCGC GCTCCTGGGC CTGTTTCTTC TCCTGCTCCT GCTTGCGCAT 40680

CAGGGCCTGG TGCCGTCGGG CTGCTTCACG CATCGAATCC CAGTCGCCGG CCAGCTCGGG 4 0740

ATGCTCCGCG CGCATCTTGC GCGTCGCCAG TTCCTCGATC TTGGGCGCGT GAATGCCCAT 40800

GCCTTCCTTG ATTTCGCGCA CCATGTCCAG CCGCGTGTGC AGGGTCTGCA AGCGGGCTTG 4 08 60

CTGTTGGGCC TGCTGCTGCT GCCAGGCGGC CTTTGTACGC GGCAGGGACA GCAAGCCGGG 40920

GGCATTGGAC TGTAGCTGCT GCAAACGCGC CTGCTGACGG TCTACGAGCT GTTCTAGGCG 40980

GTCCTCGATG CGCTCCACCT GGTCATGCTT TGCCTGCACG TAGAGCGCAA GGGTCTGCTG 4104 0

GTAGGTCTGC TCGATGGGCG CGGATTCTAA GAGGGCCTGC TGTTCCGTCT CGGCCTCCTG 41100

GGCCGCCTGT AGCAAATCCT CGCCGCTGTT GCCGCTGGAC TGCTTTACTG CCGGGGACTG 41160

CTGTTGCCCT GCTCGCGCCG TCGTCGCAGT TCGGCTTGCC CCCACTCGAT TGACTGCTTC 41220

ATTTCGAGCC GCAGCGATGC GATCTCGGAT TGCGTCAACG GACGGGGCAG CGCGGAGGTG 41280

TCCGGCTTCT CCTTGGGTGA GTCGGTCGAT GCCATAGCCA AAGGTTTCCT TCCAAAATGC 41340

GTCCATTGCT GGACCGTGTT TCTCATTGAT GCCCGCAAGC ATCTTCGGCT TGACCGCCAG 414 00

GTCAAGCGCG CCTTCATGGG CGGTCATGAC GGACGCCGCC ATGACCTTGC CGCCGTTGTT 414 60

CTCGATGTAG CCGCGTAATG AGGCAATGGT GCCGCCCATC GTCAGCGTGT CATCGACAAC 41520

GATGTACTTC TGGCCGGGGA TCACCTCCCC CTCGAAAGTC GGGTTGAACG CCAGGCGATG 41580

ATCTGAACCG GCTCCGGTTC GGGCGACCTT CTCCCGCTGC ACAATGTCCG TTTCGACCTC 41640

AAGGCCAAGG CGGTCGGCCA GAACGACCGC CATCATGGCC GGAATCTTGT TGTTCCCCGC 417 00

CGCCTCGACG GCGAGGACTG GAACGATGCG GGGCTTGTCG TCGCCGATCA GCGTCTTGAG 417 60

CTGGGCAACA GTGTCGTCCG AAATCAGGCG CTCGACCAAA TTAAGCGCCG CTTCCGCGTC 41820

GCCCTGCTTC GCAGCCTGGT ATTCAGGCTC GTTGGTCAAA GAACCAAGGT CGCCGTTGCG 41880

AACCACCTTC GGGAAGTCTC CCCACGGTGC GCGCTCGGCT CTGCTGTAGC TGCTCAAGAC 41940

GCCTCCCTTT TTAGCCGCTA AAACTCTAAC GAGTGCGCCC GCGACTCAAC TTGACGCTTT 4 2000

CGGCACTTAC CTGTGCCTTG CCACTTGCGT CATAGGTGAT GCTTTTCGCA CTCCCGATTT 4 2060

CAGGTACTTT ATCGAAATCT GACCGGGCGT GCATTACAAA GTTCTTCCCC ACCTGTTGGT 42120

AAATGCTGCC GCTATCTGCG TGGACGATGC TGCCGTCGTG GCGCTGCGAC TTATCGGCCT 4 2180

TTTGGGCCAT ATAGATGTTG TAAATGCCAG GTTTCAGGGC CCCGGCTTTA TCTACCTTCT 4 2240

GGTTCGTCCA TGCGCCTTGG TTCTCGGTCT GGACAATTCT TTGCCCATTC ATGACCAGGA 42300

GGCGGTGTTT CATTGGGTGA CTCCTGACGG TTGCCTCTGG TGTTAAACGT GTCCTGGTCG 4 2360

CTTGCCGGCT AAAAAAAAGC CGACCTCGGC AGTTCGAGGC CGGCTTTCCC TAGAGCCGGG 42420

CGCGTCAAGG TTGTTCCATC TATTTTAGTG AACTGCGTTC GATTTATCAG TTACTTTCCT 42480

CCCGCTTTGT GTTTCCTCCC ACTCGTTTCC GCGTCTAGCC GACCCCTCAA CATAGCGGCC 4 2540

TCTTCTTGGG CTGCCTTTGC CTCTTGCCGC GCTTCGTCAC GCTCGGCTTG CACCGTCGTA 42600

AAGCGCTCGG CCTGCCTGGC CGCCTCTTGC GCCGCCAACT TCCTTTGCTC CTGGTGGGCC 42660

TCGGCGTCGG CCTGCGCCTT CGCTTTCACC GCTGCCAACT CCGTGCGCAA ACTCTCCGCT 42720

TCGCGCCTGG TGGCGTCGCG CTCGCCGCGA AGCGCCTGCA TTTCCTGGTT GGCCGCGTCC 42780

AGGGTCTTGC GGCTCTCTTC TTTGAATGCG CGGGCGTCCT GGTGAGCGTA GTCCAGCTCG 4 2840

GCGCGCAGCT CCTGCGCTCG ACGCTCCACC TCGTCGGCCC GCTGCGTCGC CAGCGCGGCC 42900

CGCTGCTCGG CTCCTGCCAG GGCGGTGCGT GCTTCGGCCA GGGCTTGCCG CTGGCGTGCG 4 2960

GCCAGCTCGG CCGCCTCGGC GGCCTGCTGC TCTAGCAATG TAACGCGCGC CTGGGCTTCT 43020TCCAGCTCGC GGGCCTGCGC CTCGAAGGCG TCGGCCAGCT CCCCGCGCAC GGCTTCCAAC 43080

TCGTTGCGCT CACGATCCCA GCCGGCTTGC GCTGCCTGCA ACGATTCATT GGCAAGGGCC 43140

TGGGCGGCTT GCCAGAGGGC GGCCACGGCC TGGTTGCCGG CCTGCTGCAC CGCGTCCGGC 4 3200

ACCTGGACTG CCAGCGGGGC GGCCTGCGCC GTGCGCTGGC GTCGCCATTC GCGCATGCCG 4 3260

GCGCTGGCGT CGTTCATGTT GACGCGGGCG GCCTTACGCA CTGCATCCAC GGTCGGGAAG 4 3320

TTCTCCCGGT CGCCTTGCTC GAACAGCTCG TCCGCAGCCG CAAAAATGCG GTCGCGCGTC 43380

TCTTTGTTCA GTTCCATGTT GGCTCCGGTA ATTGGTAAGA ATAATAATAC TCTTACCTAC 4 3440

CTTATCAGCG CAAGAGTTTA GCTGAACAGT TCTCGACTTA ACGGCAGGTT TTTTAGCGGC 43500

TGAAGGGCAG GCAAAAAAAG CCCCGCACGG TCGGCGGGGG CAAAGGGTCA GCGGGAAGGG 43560

GATTAGCGGG CGTCGGGCTT CTTCATGCGT CGGGGCCGCG CTTCTTGGGA TGGAGCACGA 4 3620

CGAAGCGCGC ACGCGCATCG TCCTCGGCCC TATCGGCCCG CGTCGCGGTC AGGAACTTGT 4 3680

CGCGCGCTAG GTCCTCCCTG GTGGGCACCA GGGGCATGAA CTCGGCCTGC TCGATGTAGG 4 3740

TCCACTCCAT GACCGCATCG CAGTCGAGGC CGCGTTCCTT CACCGTCTCT TGCAGGTCGC 4 3800

GGTACGCCCG CTCGTTGAGC GGCTGGTAAC GGGCCAATTG GTCGTAAATG GCTGTCGGCC 4 3860

ATGAGCGGCC TTTCCTGTTG AGCCAGCAGC CGACGACGAA GCCGGCAATG CAGGCCCCTG 4 3920

GCACAACCAG GCCGACGCCG GGGGCAGGGG ATGGCAGCAG CTCGCCAACC AGGAACCCCG 4 3980

CCGCGATGAT GCCGATGCCG GTCAACCAGC CCTTGAAACT ATCCGGCCCC GAAACACCCC 4 4 040

TGCGCATTGC CTGGATGCTG CGCCGGATAG CTTGCAACAT CAGGAGCCGT TTCTTTTGTT 4 4100

CGTCAGTCAT GGTCCGCCCT CACCAGTTGT TCGTATCGGT GTCGGACGAA CTGAAATCGC 4 4160

AAGAGCTGCC GGTATCGGTC CAGCCGCTGT CCGTGTCGCT GCTGCCGAAG CACGGCGAGG 44220

GGTCCGCGAA CGCCGCAGAC GGCGTATCCG GCCGCAGCGC ATCGCCCAGC ATGGCCCCGG 4 4 280

TCAGCGAGCC GCCGGCCAGG TAGCCCAGCA TGGTGCTGTT GGTCGCCCCG GCCACCAGGG 4 4 340

CCGACGTGAC GAAATCGCCG TCATTCCCTC TGGATTGTTC GCTGCTCGGC GGGGCAGTGC 4 4 400

GCCGCGCCGG CGGCGTCGTG GATGGCTCGG GTTGGCTGGC CTGCGACGGC CGGCGAAAGG 4 4460

TGCGCAGCAG CTCGTTATCG ACCGGCTGCG GCGTCGGGGC CGCCGCCTTG CGCTGCGGTC 4 4 520

GGTGTTCCTT CTTCGGCTCG CGCAGCTTGA ACAGCATGAT CGCGGAAACC AGCAGCAACG 4 4580

CCGCGCCTAC GCCTCCCGCG ATGTAGAACA GCATCGGATT CATTCTTCGG TCCTCCTTGT 4 4 640

AGCGGAACCG TTGTCTGTGC GGCGCGGGTG GCCCGCGCCG CTGTCTTTGG GGATCAGCCC 44700

TCGATGAGCG CGACCAGTTT CACGTCGGCA AGGTTCGCCT CGAACTCCTG GCCGTCGTCC 4 4760

TCGTACTTCA ACCAGGCATA GCCTTCCGCC GGCGGCCGAC GGTTGAGGAT AAGGCGGGCA 4 4 820

GGGCGCTCGT CGTGCTCGAC CTGGACGATG GCCTTTTTCA GCTTGTCCGG GTCCGGCTCC 44880

TTCGCGCCCT TTTCCTTGGC GTCCTTACCG TCCTGGTCGC CGTCCTCGCC GTCCTGGCCG 4 4 940

TCGCCGGCCT CCGCGTCACG CTCGGCATCA GTCTGGCCGT TGAAGGCATC GACGGTGTTG 4 5000

GGATCGCGGC CCTTCTCGTC CAGGAACTCG CGCAGCAGCT TGACCGTGCC GCGCGTGATT 4 5060

TCCTGGGTGT CGTCGTCAAG CCACGCCTCG ACTTCCTCCG GGCGCTTCTT GAAGGCCGTC 45120

ACCAGCTCGT TCACCACGGT CACGTCGCGC ACGCGGCCGG TGTTGAACGC ATCGGCGATC 4 5180

TTCTCCGGCA GGTCCAGCAG CGTGACGTGC TGGGTGATGA ACGCCGGCGA CTTGCCGATT 4 5240

TCCTTGGCGA TATCGCCTTT CTTCTTGCCC TTCGCCAGCT CGCGGCCAAT GAAGTCGGCA 4 5300

ATTTCGCGCG GGGTCAGCTC GTTGCGTTGC AGGTTCTCGA TAACCTGGTC GGCTTCGTTG 45360

TAGTCGTTGT CGATGAACGC CGGGATGGAC TTCTTGCCGG CCCACTTCGA GCCACGGTAG 4 5420

CGGCGGGCGC CGTGATTGAT GATATAGCGG CCCGGCTGCT CCTGGTTCTC GCGCACCGAA 4 5480

ATGGGTGACT TCACCCCGCG CTCTTTGATC GTGGCACCGA TTTCCGCGAT GCTCTCCGGG 4 5540

GAAAAGCCGG GGTTGTCGGC CGTCCGCGGC TGATGCGGAT CTTCGTCGAT CAGGTCCAGG 4 5600

TCCAGCTCGA TAGGGCCGGA ACCGCCCTGA GACGCCGCAG GAGCGTCCAG GAGGCTCGAC 4 5660

AGGTCGCCGA TGCTATCCAA CCCCAGGCCG GACGGCTGCG CCGCGCCTGC GGCTTCCTGA 45720

GCGGCCGCAG CGGTGTTTTT CTTGGTGGTC TTGGCTTGAG CCGCAGTCAT TGGGAAATCT 4 5780

CCATCTTCGT GAACACGTAA TCAGCCAGGG CGCGAACCTC TTTCGATGCC TTGCGCGCGG 4 5840

CCGTTTTCTT GATCTTCCAG ACCGGCACAC CGGATGCGAG GGCATCGGCG ATGCTGCTGC 4 5900

GCAGGCCAAC GGTGGCCGGA ATCATCATCT TGGGGTACGC GGCCAGCAGC TCGGCTTGGT 4 5960

GGCGCGCGTG GCGCGGATTC CGCGCATCGA CCTTGCTGGG CACCATGCCA AGGAATTGCA 4 6020

GCTTGGCGTT CTTCTGGCGC ACGTTCGCAA TGGTCGTGAC CATCTTCTTG ATGCCCTGGA 4 6080

TGCTGTACGC CTCAAGCTCG ATGGGGGACA GCACATAGTC GGCCGCGAAG AGGGCGGCCG 4 6140

CCAGGCCGAC GCCAAGGGTC GGGGCCGTGT CGATCAGGCA CACGTCGAAG CCTTGGTTCG 4 6200

CCAGGGCCTT GATGTTCGCC CCGAACAGCT CGCGGGCGTC GTCCAGCGAC AGCCGTTCGG 4 6260

CGTTCGCCAG TACCGGGTTG GACTCGATGA GGGCGAGGCG CGCGGCCTGG CCGTCGCCGG 4 6320

CTGCGGGTGC GGTTTCGGTC CAGCCGCCGG CAGGGACAGC GCCGAACAGC TTGCTTGCAT 4 6380

GCAGGCCGGT AGCAAAGTCC TTGAGCGTGT AGGACGCATT GCCCTGGGGG TCCAGGTCGA 4 6440

TCACGGCAAC CCGCAAGCCG CGCTCGAAAA AGTCGAAGGC AAGATGCACA AGGGTCGAAG 4 6500

TCTTGCCGAC GCCGCCTTTC TGGTTGGCCG TGACCAAAGT TTTCATCGTT TGGTTTCCTG 4 6560TTTTTTCTTG GCGTCCGCTT CCCACTTCCG GACGATGTAC GCCTGATGTT CCGGCAGAAC 46620

CGCCGTTACC CGCGCGTACC CCTCGGGCAA GTTCTTGTCC TCGAACGCGG CCCACACGCG 46680

ATGCACCGCT TGCGACACTG CGCCCCTGGT CAGTCCCAGC GACGTTGCGA ACGTCGCCTG 46740

TGGCTTCCCA TCGACTAAGA CGCCCCGCGC TATCTCGATG GTCTGCTGCC CCACTTCCAG 46800

CCCCTGGATC GCCTCCTGGA ACTGGCTTTC GGTAAGCCGT TTCTTCATGG ATAACACCCA 46860

TAATTTGCTC CGCGCCTTGG TTGAACATAG CGGTGACAGC CGCCAGCACA TGAGAGAAGT 46920

TTAGCTAAAC ATTTCTCGCA CGTCAACACC TTTAGCCGCT AAAACTCGTC CTTGGCGTAA 46980

CAAAACAAAA GCCCGGAAAC CGGGCTTTCG TCTCTTGCCG CTTATGGCTC TGCACCCGGC 4 7040

TCCATCACCA ACAGGTCGCG CACGCGCTTC ACTCGGTTGC GGATCGACAC TGCCAGCCCA 4 7100

ACAAAGCCGG TTGCCGCCGC CGCCAGGATC GCGCCGATGA TGCCGGCCAC ACCGGCCATC 4 7160

GCCCACCAGG TCGCCGCCTT CCGGTTCCAT TCCTGCTGGT ACTGCTTCGC AATGCTGGAC 4 7220

CTCGGCTCAC CATAGGCTGA CCGCTCGATG GCGTATGCCG CTTCTCCCCT TGGCGTAAAA 4 7 280

CCCAGCGCCG CAGGCGGCAT TGCCATGCTG CCCGCCGCTT TCCCGACCAC GACGCGCGCA 4 7 340

CCAGGCTTGC GGTCCAGACC TTCGGCCACG GCGAGCTGCG CAAGGACATA ATCAGCCGCC 47400

GACTTGGCTC CACGCGCCTC GATCAGCTCT TGCACTCGCG CGAAATCCTT GGCCTCCACG 4 74 60

GCCGCCATGA ATCGCGCACG CGGCGAAGGC TCCGCAGGGC CGGCGTCGTG ATCGCCGCCG 4 7520

AGAATGCCCT TCACCAAGTT CGACGACACG AAAATCATGC TGACGGCTAT CACCATCATG 4 7 580

CAGACGGATC GCACGAACCC GCTGAATTGA ACACGAGCAC GGCACCCGCG ACCACTATGC 4 7 640

CAAGAATGCC CAAGGTAAAA ATTGCCGGCC CCGCCATGAA GTCCGTGAAT GCCCCGACGG 47700

CCGAAGTGAA GGGCAGGCCG CCACCCAGGC CGCCGCCCTC ACTGCCCGGC ACCTGGTCGC 4 77 60

TGAATGTCGA TGCCAGCACC TGCGGCACGT CAATGCTTCC GGGCGTCGCG CTCGGGCTGA 4 7820

TCGCCCATCC CGTTACTGCC CCGATCCCGG CAATGGCAAG GACTGCCAGC GCTGCCATTT 4 7 880

TTGGGGTGAG GCCGTTCGCG GCCGAGGGGC GCAGCCCCTG GGGGGATGGG AGGCCCGCGT 4 7940

TAGCGGGCCG GGAGGGTTCG AGAAGGGGGG GCACCCCCCT TCGGCGTGCG CGGTCACGCG 4 8000

CACAGGGCGC AGCCCTGGTT AAAAACAAGG TTTATAAATA TTGGTTTAAA AGCAGGTTAA 4 8060

AAGACAGGTT AGCGGTGGCC GAAAAACGGG CGGAAACCCT TGCAAATGCT GGATTTTCTG 4 8120

CCTGTGGACA GCCCCTCAAA TGTCAATAGG TGCGCCCCTC ATCTGTCAGC ACTCTGCCCC 4 8180

TCAAGTGTCA AGGATCGCGC CCCTCATCTG TCAGTAGTCG CGCCCCTCAA GTGTCAATAC 4 824 0

CGCAGGGCAC TTATCCCCAG GCTTGTCCAC ATCATCTGTG GGAAACTCGC GTAAAATCAG 4 8300

GCGTTTTCGC CGATTTGCGA GGCTGGCCAG CTCCACGTCG CCGGCCGAAA TCGAGCCTGC 4 8360

CCCTCATCTG TCAACGCCGC GCCGGGTGAG TCGGCCCCTC AAGTGTCAAC GTCCGCCCCT 4 8420

CATCTGTCAG TGAGGGCCAA GTTTTCCGCG AGGTATCCAC AACGCCGGCG GCCGCGGTGT 4 84 80

CTCGCACACG GCTTCGACGG CGTTTCTGGC GCGTTTGCAG GGCCATAGAC GGCCGCCAGC 4 8540

CCAGCGGCGA GGGCAACCAG CCCGGTGAGC GTCGGAAAGG CGCTGGAAGC CCCGTAGCGA 4 8 600

CGCGGAGAGG GGCGAGACAA GCCAAGGGCG CAGGCTCGAT GCGCAGCACG ACATAGCCGG 4 8 660

TTCTCGCAAG GACGAGAATT TCCCTGCGGT GCCCCTCAAG TGTCAATGAA AGTTTCCAAC 4 8720

GCGAGCCATT CGCGAGAGCC TTGAGTCCAC GCTAGATGAG AGCTTTGTTG TAGGTGGACC 4 87 80

AGTTGGTGAT TTTGAACTTT TGCTTTGCCA CGGAACGGTC TGCGTTGTCG GGAAGATGCG 4 8840

TGATCTGATC CTTCAACTCA GCAAAAGTTC GATTTATTCA ACAAAGCCAC GTTGTGTCTC 4 8900

AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT 48960

CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAAC 4 9015

<210> 96

<211> 48997

<212> DNA

<213> VETOR

<400> 96

GTCTTGCTCG ACTCTAGAGC TCGTTCCTCGCGGGGAAGCT TACAATAATG TGTGTTGTTATTTCGTCATA AATCCCGGCC TCCGTAACCCGCGGAACGGG AATATCGAGA TGCCGGGCTGACAGGTACTC CAGCTGATTG ATTATCTGCTTGCGAATGAT TACTTGAGCG CGATCGGGCAAGTGCTACAA GGCACCTTTC AGTAACGAGCAAATGGAGCG CAGTAGTCCA TCGAGGGCGGCTCGCACAGC CTCCAGATCC GATCGAGGGTCATTGCTTGA GAGTATTCCG ATGGACTGAACATCTATTTC GAGAAAGCCC CCGATGCGGC

AGGCCTCGAG GCCTCGAGGA ACGGTACCTG 60AGTCTTGTTG CCTGTCATCG TCTGACTGAC 120AGCTTTGGGC AAGCTCACGG ATTTGATCCG 180AACGCTGCAG TTCCAGCTTT CCCTTTCGGG 240GAAGGGTCTT GGTTCCACCT CCTGGCACAA 300TCCAATTTTC TCCCGTCAGG TGCGTGGTCA 360GACCGTCGAT CCGTCGCCGG GATACGGACA 420CGAAAGCCTC GCCAAAAGCA ATACGTTCAT 480CTTCGGCGTA GGCAGATAGA AGCATGGATA 540GTATGGCTTC CATCTTTTCT CGTGTGTCTG 600GCACCGCAAC GCGAATTGCC ATACTATCCG 660AAAGTCCCAG CAGGCGCGCT TGATAGGAAA AGGTTTCATA CTCGGCCGAT CGCAGACGGG 7 20

CACTCACGAC CTTGAACCCT TCAACTTTCA GGGATCGATG CTGGTTGATG GTAGTCTCAC 780

TCGACGTGGC TCTGGTGTGT TTTGACATAG CTTCCTCCAA AGAAAGCGGA AGGTCTGGAT 840

ACTCCAGCAC GAAATGTGCC CGGGTAGACG GATGGAAGTC TAGCCCTGCT CAATATGAAA 900

TCAACAGTAC ATTTACAGTC AATACTGAAT ATACTTGCTA CATTTGCAAT TGTCTTATAA 960

CGAATGTGAA ATAAAAATAG TGTAACAACG CTTTTACTCA TCGATAATCA CAAAAACATT 1020

TATACGAACA AAAATACAAA TGCACTCCGG TTTCACAGGA TAGGCGGGAT CAGAATATGC 1080

AACTTTTGAC GTTTTGTTCT TTCAAAGGGG GTGCTGGCAA AACCACCGCA CTCATGGGCC 114 0

TTTGCGCTGC TTTGGCAAAT GACGGTAAAC GAGTGGCCCT CTTTGATGCC GACGAAAACC 1200

GGCCTCTGAC GCGATGGAGA GAAAACGCCT TACAAAGCAG TACTGGGATC CTCGCTGTGA 1260

AGTCTATTCC GCCGACGAAA TGCCCCTTCT TGAAGCAGCC TATGAAAATG CCGAGCTCGA 1320

AGGATTTGAT TATGCGTTGG CCGATACGCG TGGCGGCTCG AGCGAGCTCA ACAACACAAT 1380

CATCGCTAGC TCAAACCTGC TTCTGATCCC CACCATGCTA ACGCCGCTCG ACATCGATGA 14 40

GGCACTATCT ACCTACCGCT ACGTCATCGA GCTGCTGTTG AGTGAAAATT TGGCAATTCC 1500

TACAGCTGTT TTGCGCCAAC GCGTCCCGGT CGGCCGATTG ACAACATCGC AACGCAGGAT 1560

GTCAGAGACG CTAGAGAGCC TTCCAGTTGT ACCGTCTCCC ATGCATGAAA GAGATGCATT 1620

TGCCGCGATG AAAGAACGCG GCATGTTGCA TCTTACATTA CTAAACACGG GAACTGATCC 1680

GACGATGCGC CTCATAGAGA GGAATCTTCG GATTGCGATG GAGGAAGTCG TGGTCATTTC 17 4 0

GAAACTGATC AGCAAAATCT TGGAGGCTTG AAGATGGCAA TTCGCAAGCC CGCATTGTCG 1800

GTCGGCGAAG CACGGCGGCT TGCTGGTGCT CGACCCGAGA TCCACCATCC CAACCCGACA 18 60

CTTGTTCCCC AGAAGCTGGA CCTCCAGCAC TTGCCTGAAA AAGCCGACGA GAAAGACCAG 1920

CAACGTGAGC CTCTCGTCGC CGATCACATT TACAGTCCCG ATCGACAACT TAAGCTAACT 1980

GTGGATGCCC TTAGTCCACC TCCGTCCCCG AAAAAGCTCC AGGTTTTTCT TTCAGCGCGA 204 0

CCGCCCGCGC CTCAAGTGTC GAAAACATAT GACAACCTCG TTCGGCAATA CAGTCCCTCG 2100

AAGTCGCTAC AAATGATTTT AAGGCGCGCG TTGGACGATT TCGAAAGCAT GCTGGCAGAT 2160

GGATCATTTC GCGTGGCCCC GAAAAGTTAT CCGATCCCTT CAACTACAGA AAAATCCGTT 2220

CTCGTTCAGA CCTCACGCAT GTTCCCGGTT GCGTTGCTCG AGGTCGCTCG AAGTCATTTT 2280

GATCCGTTGG GGTTGGAGAC CGCTCGAGCT TTCGGCCACA AGCTGGCTAC CGCCGCGCTC 234 0

GCGTCATTCT TTGCTGGAGA GAAGCCATCG AGCAATTGGT GAAGAGGGAC CTATCGGAAC 24 00

CCCTCACCAA ATATTGAGTG TAGGTTTGAG GCCGCTGGCC GCGTCCTCAG TCACCTTTTG 24 60

AGCCAGATAA TTAAGAGCCA AATGCAATTG GCTCAGGCTG CCATCGTCCC CCCGTGCGAA 2520

ACCTGCACGT CCGCGTCAAA GAAATAACCG GCACCTCTTG CTGTTTTTAT CAGTTGAGGG 2580

CTTGACGGAT CCGCCTCAAG TTTGCGGCGC AGCCGCAAAA TGAGAACATC TATACTCCTG 264 0

TCGTAAACCT CCTCGTCGCG TACTCGACTG GCAATGAGAA GTTGCTCGCG CGATAGAACG 27 00

TCGCGGGGTT TCTCTAAAAA CGCGAGGAGA AGATTGAACT CACCTGCCGT AAGTTTCACC 27 60

TCACCGCCAG CTTCGGACAT CAAGCGACGT TGCCTGAGAT TAAGTGTCCA GTCAGTAAAA 2820

CAAAAAGACC GTCGGTCTTT GGAGCGGACA ACGTTGGGGC GCACGCGCAA GGCAACCCGA 2880

ATGCGTGCAA GAAACTCTCT CGTACTAAAC GGCTTAGCGA TAAAATCACT TGCTCCTAGC 294 0

TCGAGTGCAA CAACTTTATC CGTCTCCTCA AGGCGGTCGC CACTGATAAT TATGATTGGA 3000

ATATCAGACT TTGCCGCCAG ATTTCGAACG ATCTCAAGCC CATCTTCACG ACCTAAATTT 3060

AGATCAACAA CCACGACATC GACCGTCGCG GAAGAGAGTA CTCTAGTGAA CTGGGTGCTG 3120

TCGGCTACCG CGGTCACTTT GAAGGCGTGG ATCGTAAGGT ATTCGATAAT AAGATGCCGC 3180

ATAGCGACAT CGTCATCGAT AAGAAGAACG TGTTTCAACG GCTCACCTTT CAATCTAAAA 3240

TCTGAACCCT TGTTCACAGC GCTTGAGAAA TTTTCACGTG AAGGATGTAC AATCATCTCC 3300

AGCTAAATGG GCAGTTCGTC AGAATTGCGG CTGACCGCGG ATGACGAAAA TGCGAACCAA 3360

GTATTTCAAT TTTATGACAA AAGTTCTCAA TCGTTGTTAC AAGTGAAACG CTTCGAGGTT 3420

ACAGCTACTA TTGATTAAGG AGATCGCCTA TGGTCTCGCC CCGGCGTCGT GCGTCCGCCG 3480

CGAGCCAGAT CTCGCCTACT TCATAAACGT CCTCATAGGC ACGGAATGGA ATGATGACAT 354 0

CGATCGCCGT AGAGAGCATG TCAATCAGTG TGCGATCTTC CAAGCTAGCA CCTTGGGCGC 3600

TACTTTTGAC AAGGGAAAAC AGTTTCTTGA ATCCTTGGAT TGGATTCGCG CCGTGTATTG 3660

TTGAAATCGA TCCCGGATGT CCCGAGACGA CTTCACTCAG ATAAGCCCAT GCTGCATCGT 3720

CGCGCATCTC GCCAAGCAAT ATCCGGTCCG GCCGCATACG CAGACTTGCT TGGAGCAAGT 3780

GCTCGGCGCT CACAGCACCC AGCCCAGCAC CGTTCTTGGA GTAGAGTAGT CTAACATGAT 3840

TATCGTGTGG AATGACGAGT TCGAGCGTAT CTTCTATGGT GATTAGCCTT TCCTGGGGGG 3900

GGATGGCGCT GATCAAGGTC TTGCTCATTG TTGTCTTGCC GCTTCCGGTA GGGCCACATA 3960

GCAACATCGT CAGTCGGCTG ACGACGCATG CGTGCAGAAA CGCTTCCAAA TCCCCGTTGT 4 020

CAAAATGCTG AAGGATAGCT TCATCATCCT GATTTTGGCG TTTCCTTCGT GTCTGCCACT 4 080

GGTTCCACCT CGAAGCATCA TAACGGGAGG AGACTTCTTT AAGACCAGAA ACACGCGAGC 4140

TTGGCCGTCG AATGGTCAAG CTGACGGTGC CCGAGGGAAC GGTCGGCGGC AGACAGATTT 4 200GTAGTCGTTC ACCACCAGGA AGTTCAGTGG CGCAGAGGGG GTTACGTGGT CCGACATCCT 4260

GCTTTCTCAG CGCGCCCGCT AAAATAGCGA TATCTTCAAG ATCATCATAA GAGACGGGCA 4320

AAGGCATCTT GGTAAAAATG CCGGCTTGGC GCACAAATGC CTCTCCAGGT CGATTGATCG 4380

CAATTTCTTC AGTCTTCGGG TCATCGAGCC ATTCCAAAAT CGGCTTCAGA AGAAAGCGTA 4440

GTTGCGGATC CACTTCCATT TACAATGTAT CCTATCTCTA AGCGGAAATT TGAATTCATT 4500

AAGAGCGGCG GTTCCTCCCC CGCGTGGCGC CGCCAGTCAG GCGGAGCTGG TAAACACCAA 4560

AGAAATCGAG GTCCCGTGCT ACGAAAATGG AAACGGTGTC ACCCTGATTC TTCTTCAGGG 4620

TTGGCGGTAT GTTGATGGTT GCCTTAAGGG CTGTCTCAGT TGTCTGCTCA CCGTTATTTT 4680

GAAAGCTGTT GAAGCTCATC CCGCCACCCG AGCTGCCGGC GTAGGTGCTA GCTGCCTGGA 4740

AGGCGCCTTG AACAACACTC AAGAGCATAG CTCCGCTAAA ACGCTGCCAG AAGTGGCTGT 4800

CGACCGAGCC CGGCAATCCT GAGCGACCGA GTTCGTCCGC GCTTGGCGAT GTTAACGAGA 4860

TCATCGCATG GTCAGGTGTC TCGGCGCGAT CCCACAACAC AAAAACGCGC CCATCTCCCT 4920

GTTGCAAGCC ACGCTGTATT TCGCCAACAA CGGTGGTGCC ACGATCAAGA AGCACGATAT 4980

TGTTCGTTGT TCCACGAATA TCCTGAGGCA AGACACACTT TACATAGCCT GCCAAATTTG 5040

TGTCGATTGC GGTTTGCAAG ATGCACGGAA TTATTGTCCC TTGCGTTACC ATAAAATCGG 5100

GGTGCGGCAA GAGCGTGGCG CTGCTGGGCT GCAGCTCGGT GGGTTTCATA CGTATCGACA 5160

AATCGTTCTC GCCGGACACT TCGCCATTCG GCAAGGAGTT GTCGTCACGC TTGCCTTCTT 5220

GTCTTCGGCC CGTGTCGCCC TGAATGGCGC GTTTGCTGAC CCCTTGATCG CCGCTGCTAT 5280

ATGCAAAAAT CGGTGTTTCT TCCGGCCGTG GCTCATGCCG CTCCGGTTCG CCCCTCGGCG 5340

GTAGAGGAGC AGCAGGCTGA ACAGCCTCTT GAACCGCTGG AGGATCCGGC GGCACCTCAA 5400

TCGGAGCTGG ATGAAATGGC TTGGTGTTTG TTGCGATCAA AGTTGACGGC GATGCGTTCT 5460

CATTCACCTT CTTTTGGCGC CCACCTAGCC AAATGAGGCT TAATGATAAC GCGAGAACGA 5520

CACCTCCGAC GATCAATTTC TGAGACCCCG AAAGACGCCG GCGATGTTTG TCGGAGACCA 5580

GGGATCCAGA TGCATCAACC TCATGTGCCG CTTGCTGACT ATCGTTATTC ATCCCTTCGC 5640

CCCCTTCAGG ACGCGTTTCA CATCGGGCCT CACCGTGCCC GTTTGCGGCC TTTGGCCAAC 5700

GGGATCGTAA GCGGTGTTCC AGATACATAG TACTGTGTGG CCATCCCTCA GACGCCAACC 5760

TCGGGAAACC GAAGAAATCT CGACATCGCT CCCTTTAACT GAATAGTTGG CAACAGCTTC 5820

CTTGCCATCA GGATTGATGG TGTAGATGGA GGGTATGCGT ACATTGCCCG GAAAGTGGAA 5880

TACCGTCGTA AATCCATTGT CGAAGACTTC GAGTGGCAAC AGCGAACGAT CGCCTTGGGC 5940

GACGTAGTGC CAATTACTGT CCGCCGCACC AAGGGCTGTG ACAGGCTGAT CCAATAAATT 6000

CTCAGCTTTC CGTTGATATT GTGCTTCCGC GTGTAGTCTG TCCACAACAG CCTTCTGTTG 6060

TGCCTCCCTT CGCCGAGCCG CCGCATCGTC GGCGGGGTAG GCGAATTGGA CGCTGTAATA 6120

GAGATCGGGC TGCTCTTTAT CGAGGTGGGA CAGAGTCTTG GAACTTATAC TGAAAACATA 6180

ACGGCGCATC CCGGAGTCGC TTGCGGTTAG CACGATTACT GGCTGAGGCG TGAGGACCTG 6240

GCTTGCCTTG AAAAATAGAT AATTTCCCCG CGGTAGGGCT GCTAGATCTT TGCTATTTGA 6300

AACGGCAACC GCTGTCACCG TTTCGTTCGT GGCGAATGTT ACGACCAAAG TAGCTCCAAC 6360

CGCCGTCGAG AGGCGCACCA CTTGATCGGG ATTGTAAGCC AAATAACGCA TGCGCGGATC 6420

TAGCTTGCCC GCCATTGGAG TGTCTTCAGC CTCCGCACCA GTCGCAGCGG CAAATAAACA 6480

TGCTAAAATG AAAAGTGCTT TTCTGATCAT GGTTCGCTGT GGCCTACGTT TGAAACGGTA 6540

TCTTCCGATG TCTGATAGGA GGTGACAACC AGACCTGCCG GGTTGGTTAG TCTCAATCTG 6600

CCGGGCAAGC TGGTCACCTT TTCGTAGCGA ACTGTCGCGG TCCACGTACT CACCACAGGC 6660

ATTTTGCCGT CAACGACGAG GGTCCTTTTA TAGCGAATTT GCTGCGTGCT TGGAGTTACA 6720

TCATTTGAAG CGATGTGCTC GACCTCCACC CTGCCGCGTT TGCCAAGAAT GACTTGAGGC 6780

GAACTGGGAT TGGGATAGTT GAAGAATTGC TGGTAATCCT GGCGCACTGT TGGGGCACTG 6840

AAGTTCGATA CCAGGTCGTA GGCGTACTGA GCGGTGTCGG CATCATAACT CTCGCGCAGG 6900

CGAACGTACT CCCACAATGA GGCGTTAACG ACGGCCTCCT CTTGAGTTGC AGGCAATCGC 6960

GAGACAGACA CCTCGCTGTC AACGGTGCCG TCCGGCCGTA TCCATAGATA TACGGGCACA 7020

AGCCTGCTCA ACGGCACCAT TGTGGCTATA GCGAACGCTT GAGCAACATT TCCCAAAATC 7080

GCGATAGCTG CGACAGCTGC AATGAGTTTG GAGAGACGTC GCGCCGATTT CGCTCGCGCG 7140

GTTTGAAAGG CTTCTACTTC CTTATAGTGC TCGGCAAGGC TTTCGCGCGC CACTAGCATG 7200

GCATATTCAG GCCCCGTCAT AGCGTCCACC CGAATTGCCG AGCTGAAGAT CTGACGGAGT 7260

AGGCTGCCAT CGCCCCACAT TCAGCGGGAA GATCGGGCCT TTGCAGCTCG CTAATGTGTC 7320

GTTTGTCTGG CAGCCGCTCA AAGCGACAAC TAGGCACAGC AGGCAATACT TCATAGAATT 7380

CTCCATTGAG GCGAATTTTT GCGCGACCTA GCCTCGCTCA ACCTGAGCGA AGCGACGGTA 7440

CAAGCTGCTG GCAGATTGGG TTGCGCCGCT CCAGTAACTG CCTCCAATGT TGCCGGCGAT 7500

CGCCGGCAAA GCGACAATGA GCGCATCCCC TGTCAGAAAA AACATATCGA GTTCGTAAAG 7560

ACCAATGATC TTGGCCGCGG TCGTACCGGC GAAGGTGATT ACACCAAGCA TAAGGGTGAG 7620

CGCAGTCGCT TCGGTTAGGA TGACGATCGT TGCCACGAGG TTTAAGAGGA GAAGCAAGAG 7680

ACCGTAGGTG ATAAGTTGCC CGATCCACTT AGCTGCGATG TCCCGCGTGC GATCAAAAAT 7740atatccgacg aggatcagag gcccgatcgc gagaagcact ttcgtgagaa ttccaacggc 7 800

gtcgtaaact ccgaaggcag accagagcgt gccgtaaagg acccactgtg ccccttggaa 7860

agcaaggatg tcctggtcgt tcatcggacc gatttcggat gcgattttct gaaaaacggc 7920

ctgggtcacg gcgaacattg tatccaactg tgccggaaca gtctgcagag gcaagccggt 7 980

tacactaaac tgctgaacaa agtttgggac cgtcttttcg aagatggaaa ccacatagtc 804 0

ttggtagtta gcctgcccaa caattagagc aacaacgatg gtgaccgtga tcacccgagt 8100

gataccgcta cgggtatcga cttcgccgcg tatgactaaa ataccctgaa caataatcca 8160

aagagtgaca caggcgatca atggcgcact caccgcctcc tggatagtct caagcatcga 8220

gtccaagcct gtcgtgaagg ctacatcgaa gatcgtatga atggccgtaa acggcgccgg 8280

aatcgtgaaa ttcatcgatt ggacctgaac ttgactggtt tgtcgcataa tgttggataa 8340

aatgagctcg cattcggcga ggatgcgggc ggatgaacaa atcgcccagc cttaggggag 8400

ggcaccaaag atgacagcgg tcttttgatg ctccttgcgt tgagcggccg cctcttccgc 84 60

ctcgtgaagg ccggcctgcg cggtagtcat cgttaatagg cttgtcgcct gtacattttg 8520

aatcattgcg tcatggatct gcttgagaag caaaccattg gtcacggttg cctgcatgat 8580

attgcgagat cgggaaagct gagcagacgt atcagcattc gccgtcaagc gtttgtccat 864 0

cgtttccaga ttgtcagccg caatgccagc gctgtttgcg gaaccggtga tctgcgatcg 8700

caacaggtcc gcttcagcat cactacccac gactgcacga tctgtatcgc tggtgatcgc 87 60

acgtgccgtg gtcgacattg gcattcgcgg cgaaaacatt tcattgtcta ggtccttcgt 8820

cgaaggatac tgatttttct ggttgagcga agtcagtagt ccagtaacgc cgtaggccga 8880

cgtcaacatc gtaaccatcg ctatagtctg agtgagattc tccgcagtcg cgagcgcagt 8940

cgcgagcgtc tcagcctccg ttgccgggtc gctaacaaca aactgcgccc gcgcgggctg 9000

aatatataga aagctgcagg tcaaaactgt tgcaataagt tgcgtcgtct tcatcgtttc 9060

ctaccttatc aatcttctgc ctcgtggtga cgggccatga attcgctgag ccagccagat 9120

gagttgcctt cttgtgcctc gcgtagtcga gttgcaaagc gcaccgtgtt ggcacgcccc 9180

gaaagcacgg cgacatattc acgcatatcc cgcagatcaa attcgcagat gacgcttcca 924 0

ctttctcgtt taagaagaaa cttacggctg ccgaccgtca tgtcttcacg gatcgcctga 9300

aattcctttt cggtacattt cagtccatcg acataagccg atcgatctgc ggttggtgat 9360

ggatagaaaa tcttcgtcat acattgcgca accaagctgg ctcctagcgg cgattccaga 9420

acatgctctg gttgctgcgt tgccagtatt agcatcccgt tgttttttcg aacggtcagg 94 80

aggaatttgt cgacgacagt cgaaaattta gggtttaaca aataggcgcg aaactcatcg 9540

cagctcatca caaaacggcg gccgtcgatc atggctccaa tccgatgcag gagatatgct 9600

gcagcgggag cgcatacttc ctcgtattcg agaagatgcg tcatgtcgaa gccggtaatc 9660

gacggatcta actttacttc gtcaacttcg ccgtcaaatg cccagccaag cgcatggccc 9720

cggcaccagc gttggagccg cgctcctgcg ccttcggcgg gcccatgcaa caaaaattca 9780

cgtaaccccg cgattgaacg catttgtgga tcaaacgaga gctgacgatg gataccacgg 98 4 0

accagacggc ggttctcttc cggagaaatc ccaccccgac catcactctc gatgagagcc 9900

acgatccatt cgcgcagaaa atcgtgtgag gctgctgtgt tttctaggcc acgcaacggc 9960

gccaacccgc tgggtgtgcc tctgtgaagt gccaaatatg ttcctcctgt ggcgcgaacc 10020

agcaattcgc caccccggtc cttgtcaaag aacacgaccg tacctgcacg gtcgaccatg 10080

ctctgttcga gcatggctag aacaaacatc atgagcgtcg tcttacccct cccgataggc 1014 0

ccgaatattg ccgtcatgcc aacatcgtgc tcatgcggga tatagtcgaa aggcgttccg 10200

ccattggtac gaaatcgggc aatcgcgttg ccccagtggc ctgagctggc gccctctgga 10260

aagttttcga aagagacaaa ccctgcgaaa ttgcgtgaag tgattgcgcc agggcgtgtg 10320

cgccacttaa aattccccgg caattgggac caataggccg cttccatacc aataccttct 10380

tggacaacca cggcacctgc atccgccatt cgtgtccgag cccgcgcgcc cctgtcccca 104 4 0

agactattga gatcgtctgc atagacgcaa aggctcaaat gatgtgagcc cataacgaat 10500

tcgttgctcg caagtgcgtc ctcagcctcg gataatttgc cgatttgagt cacggcttta 10560

tcgccggaac tcagcatctg gctcgatttg aggctaagtt tcgcgtgcgc ttgcgggcga 10620

gtcaggaacg aaaaactctg cgtgagaaca agtggaaaat cgagggatag cagcgcgttg 10680

agcatgcccg gccgtgtttt tgcagggtat tcgcgaaacg aatagatgga tccaacgtaa 10740

ctgtcttttg gcgttctgat ctcgagtcct cgcttgccgc aaatgactct gtcggtataa 10800

atcgaagcgc cgagtgagcc gctgacgacc ggaaccggtg tgaaccgacc agtcatgatc 108 60

aaccgtagcg cttcgccaat ttcggtgaag agcacaccct gcttctcgcg gatgccaaga 10920

cgatgcaggc catacgcttt aagagagcca gcgacaacat gccaaagatc ttccatgttc 10980

ctgatctggc ccgtgagatc gttttccctt tttccgctta gcttggtgaa cctcctcttt 1104 0

accttcccta aagccgcctg tgggtagaca atcaacgtaa ggaagtgttc attgcggagg 11100

agttggccgg agagcacgcg ctgttcaaaa gcttcgttca ggctagcggc gaaaacacta 11160

cggaagtgtc gcggcgccga tgatggcacg tcggcatgac gtacgaggtg agcatatatt 11220

gacacatgat catcagcgat attgcgcaac agcgtgttga acgcacgaca acgcgcattg 11280CGCATTTCAG TTTCCTCAAG CTCGAATGCA ACGCCATCAA TTCTCGCAAT GGTCATGATC 11340

GATCCGTCTT CAAGAAGGAC GATATGGTCG CTGAGGTGGC CAATATAAGG GAGATAGATC 11400

TCACCGGATC TTTCGGTCGT TCCACTCGCG CCGAGCATCA CACCATTCCT CTCCCTCGTG 114 60

GGGGAACCCT AATTGGATTT GGGCTAACAG TAGCGCCCCC CCAAACTGCA CTATCAATGC 11520

TTCTTCCCGC GGTCCGCAAA AATAGCAGGA CGACGCTCGC CGCATTGTAG TCTCGCTCCA 11580

CGATGAGCCG GGCTGCAAAC CATAACGGCA CGAGAACGAC TTCGTAGAGC GGGTTCTGAA 11640

CGATAACGAT GACAAAGCCG GCGAACATCA TGAATAACCC TGCCAATGTC AGTGGCACCC 117 00

CAAGAAACAA TGCGGGCCGT GTGGCTGCGA GGTAAAGGGT CGATTCTTCC AAACGATCAG 117 60

CCATCAACTA CCGCCAGTGA GCGTTTGGCC GAGGAAGCTC GCCCCAAACA TGATAACAAT 11820

GCCGCCGACG ACGCCGGCAA CCAGCCCAAG CGAAGCCCGC CCGAACATCC AGGAGATCCC 11880

GATAGCGACA ATGCCGAGAA CAGCGAGTGA CTGGCCGAAC GGACCAAGGA TAAACGTGCA 11940

TATATTGTTA ACCATTGTGG CGGGGTCAGT GCCGCCACCC GCAGATTGCG CTGCGGCGGG 12000

TCCGGATGAG GAAATGCTCC ATGCAATTGC ACCGCACAAG CTTGGGGCGC AGCTCGATAT 12060

CACGCGCATC ATCGCATTCG AGAGCGAGAG GCGATTTAGA TGTAAACGGT ATCTCTCAAA 12120

GCATCGCATC AATGCGCACC TCCTTAGTAT AAGTCGAATA AGACTTGATT GTCGTCTGCG 12180

GATTTGCCGT TGTCCTGGTG TGGCGGTGGC GGAGCGATTA AACCGCCAGC GCCATCCTCC 1224 0

TGCGAGCGGC GCTGATATGA CCCCCAAACA TCCCACGTCT CTTCGGATTT TAGCGCCTCG 12300

TGATCGTCTT TTGGAGGCTC GATTAACGCG GGCACCAGCG ATTGAGCAGC TGTTTCAACT 12 360

TTTCGCACGT AGCCGTTTGC AAAACCGCCG ATGAAATTAC CGGTGTTGTA AGCGGAGATC 12420

GCCCGACGAA GCGCAAATTG CTTCTCGTCA ATCGTTTCGC CGCCTGCATA ACGACTTTTC 124 80

AGCATGTTTG CAGCGGCAGA TAATGATGTG CACGCCTGGA GCGCACCGTC AGGTGTCAGA 1254 0

CCGAGCATAG AAAAATTTCG AGAGTTTATT TGCATGAGGC CAACATCCAG CGAATGCCGT 12 600

GCATCGAGAC GGTGCCTGAC GACTTGGGTT GCTTGGCTGT GATCTTGCCA GTGAAGCGTT 12 660

TCGCCGGTCG TGTTGTCATG AATCGCTAAA GGATCAAAGC GACTCTCCAC CTTAGCTATC 12720

GCCGCAAGCG TAGATGTCGC AACTGATGGG GCACACTTGC GAGCAACATG GTCAAACTCA 12780

GCAGATGAGA GTGGCGTGGC AAGGCTCGAC GAACAGAAGG AGACCATCAA GGCAAGAGAA 12840

AGCGACCCCG ATCTCTTAAG CATACCTTAT CTCCTTAGCT CGCAACTAAC ACCGCCTCTC 12900

CCGTTGGAAG AAGTGCGTTG TTTTATGTTG AAGATTATCG GGAGGGTCGG TTACTCGAAA 12 960

ATTTTCAATT GCTTCTTTAT GATTTCAATT GAAGCGAGAA ACCTCGCCCG GCGTCTTGGA 13020

ACGCAACATG GACCGAGAAC CGCGCATCCA TGACTAAGCA ACCGGATCGA CCTATTCAGG 13080

CCGCAGTTGG TCAGGTCAGG CTCAGAACGA AAATGCTCGG CGAGGTTACG CTGTCTGTAA 1314 0

ACCCATTCGA TGAACGGGAA GCTTCCTTCC GATTGCTCTT GGCAGGAATA TTGGCCCATG 13200

CCTGCTTGCG CTTTGCAAAT GCTCTTATCG CGTTGGTATC ATATGCCTTG TCCGCCAGCA 13260

GAAACGCACT CTAAGCGATT ATTTGTAAAA ATGTTTCGGT CATGCGGCGG TCATGGGCTT 13320

GACCCGCTGT CAGCGCAAGA CGGATCGGTC AACCGTCGGC ATCGACAACA GCGTGAATCT 13380

TGGTGGTCAA ACCGCCACGG GAACGTCCCA TACAGCCATC GTCTTGATCC CGCTGTTTCC 134 4 0

CGTCGCCGCA TGTTGGTGGA CGCGGACACA GGAACTGTCA ATCATGACGA CATTCTATCG 13500

AAAGCCTTGG AAATCACACT CAGAATATGA TCCCAGACGT CTGCCTCACG CCATCGTACA 13560

AAGCGATTGT AGCAGGTTGT ACAGGAACCG TATCGATCAG GAACGTCTGC CCAGGGCGGG 13620

CCCGTCCGGA AGCGCCACAA GATGACATTG ATCACCCGCG TCAACGCGCG GCACGCGACG 13680

CGGCTTATTT GGGAACAAAG GACTGAACAA CAGTCCATTC GAAATCGGTG ACATCAAAGC 137 4 0

GGGGACGGGT TATCAGTGGC CTCCAAGTCA AGCCTCAATG AATCAAAATC AGACCGATTT 13800

GCAAACCTGA TTTATGAGTG TGCGGCCTAA ATGATGAAAT CGTCCTTCTA GATCGCCTCC 13860

GTGGTGTAGC AACACCTCGC AGTATCGCCG TGCTGACCTT GGCCAGGGAA TTGACTGGCA 13920

AGGGTGCTTT CACATGACCG CTCTTTTGGC CGCGATAGAT GATTTCGTTG CTGCTTTGGG 13980

CACGTAGAAG GAGAGAAGTC ATATCGGAGA AATTCCTCCT GGCGCGAGAG CCTGCTCTAT 14 04 0

CGCGACGGCA TCCCACTGTC GGGAACAGAC CGGATCATTC ACGAGGCGAA AGTCGTCAAC 14100

ACATGCGTTA TAGGCATCTT CCCTTGAAGG ATGATCTTGT TGCTGCCAAT CTGGAGGTGC 14160

GGCAGCCGCA GGCAGATGCG ATCTCAGCGC AACTTGCGGC AAAACATCTC ACTCACCTGA 14 220

AAACCACTAG CGAGTCTCGC GATCAGACGA AGGCCTTTTA CTTAACGACA CAATATCCGA 14 280

TGTCTGCATC ACAGGCGTCG CTATCCCAGT CAATACTAAA GCGGTGCAGG AACTAAAGAT 14 34 0

TACTGATGAC TTAGGCGTGC CACGAGGCCT GAGACGACGC GCGTAGACAG TTTTTTGAAA 14 400

TCATTATCAA AGTGATGGCC TCCGCTGAAG CCTATCACCT CTGCGCCGGT CTGTCGGAGA 144 60

GATGGGCAAG CATTATTACG GTCTTCGCGC CCGTACATGC ATTGGACGAT TGCAGGGTCA 14520

ATGGATCTGA GATCATCCAG AGGATTGCCG CCCTTACCTT CCGTTTCGAG TTGGAGCCAG 14580

CCCCTAAATG AGACGACATA GTCGACTTGA TGTGACAATG CCAAGAGAGA GATTTGCTTA 14 640

ACCCGATTTT TTTGCTCAAG CGTAAGCCTA TTGAAGCTTG CCGGCATGAC GTCCGCGCCG 14700

AAAGAATATC CTACAAGTAA AACATTCTGC ACACCGAAAT GCTTGGTGTA GACATCGATT 14 7 60

ATGTGACCAA GATCCTTAGC AGTTTCGCTT GGGGACCGCT CCGACCAGAA ATACCGAAGT 14 820GAACTGACGC CAATGACAGG AATCCCTTCC GTCTGCAGAT AGGTACCATC GATAGATCTG 14 880

CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 14 940

GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 15000

GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA 15060

TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 15120

GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG 15180

CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 1524 0

GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 15300

GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 15360

CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 15420

GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 154 80

ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 15540

CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 15600

GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 15660

TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 15720

AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 15780

ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 15840

GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 15900

AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 15960

GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 16020

AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 16080

ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 1614 0

GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 16200

ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 16260

CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 16320

CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 16380

AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGGGG GGGGGGGGGG 16440

GGGTTCCATT GTTCATTCCA CGGACAAAAA CAGAGAAAGG AAACGACAGA GGCCAAAAAG 16500

CTCGCTTTCA GCACCTGTCG TTTCCTTTCT TTTCAGAGGG TATTTTAAAT AAAAACATTA 16560

AGTTATGACG AAGAAGAACG GAAACGCCTT AAACCGGAAA ATTTTCATAA ATAGCGAAAA 16620

CCCGCGAGGT CCCTGTCGGA TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT 16680

ACATATCACA ACGTGCGTGG AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG 16740

TATCGTATTA ATTGATCTGC ATCAACTTAA CGTAAAAACA ACTTCAGACA ATACAAATCA 16800

GCGACACTGA ATACGGGGCA ACCTCATGTC CCCCCCCCCC CCCCCCCTGC AGGCATCGTG 168 60

GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 16920

GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 16980

GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 17 040

CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 17100

TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT 17160

ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 17220

AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 17280

AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 17340

CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 17400

CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 174 60

GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 17520

CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 17580

AGGCCCTTTC GTCTTCAAGA ATTGGTCGAC GATCTTGCTG CGTTCGGATA TTTTCGTGGA 17 640

GTTCCCGCCA CAGACCCGGA TTGAAGGCGA GATCCAGCAA CTCGCGCCAG ATCATCCTGT 177 00

GACGGAACTT TGGCGCGTGA TGACTGGCCA GGACGTCGGC CGAAAGAGCG ACAAGCAGAT 177 60

CACGCTTTTC GACAGCGTCG GATTTGCGAT CGAGGATTTT TCGGCGCTGC GCTACGTCCG 17820

CGACCGCGTT GAGGGATCAA GCCACAGCAG CCCACTCGAC CTTCTAGCCG ACCCAGACGA 17880

GCCAAGGGAT CTTTTTGGAA TGCTGCTCCG TCGTCAGGCT TTCCGACGTT TGGGTGGTTG 17940

AACAGAAGTC ATTATCGTAC GGAATGCCAA GCACTCCCGA GGGGAACCCT GTGGTTGGCA 18000

TGCACATACA AATGGACGAA CGGATAAACC TTTTCACGCC CTTTTAAATA TCCGTTATTC 18060

TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT 18120

TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT 18180

ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA 18240

CGTTGAAGGA GCCACTCAGC AAGCTGGTAC GATTGTAATA CGACTCACTA TAGGGCGAAT 18300

TGAGCGCTGT TTAAACGCTC TTCAACTGGA AGAGCGGTTA CCAGAGCTGG TCACCTTTGT 18360CCACCAAGAT GGAACTGCGG CCGCTCATTA ATTAAGTCAG GCGCGCCTCT AGTTGAAGAC 18420

ACGTTCATGT CTTCATCGTA AGAAGACACT CAGTAGTCTT CGGCCAGAAT GGCCATCTGG 184 80

ATTCAGCAGG CCTAGAAGGC CATTTAAATC CTGAGGATCT GGTCTTCCTA AGGACCCGGG 18540

ATATCGCTAT CAACTTTGTA TAGAAAAGTT GGGCCGAATT CGCCCTTGTT TAAACTTAAT 18 600

ATTTGTTTAA ACTTTTTACT AAATTCATGT AATAATTAAT GTATGCGTTA TATATATATG 18 660

TCTAGGTTTA TAATTATTCA TATGAATATG AACATAAAAA TCTAGGGCTA AAACGACTAC 18720

TATTTTGAAA ACGGAAGGAG TAGTAAGTTA TTTAAGCGGA GGGGAACCAT GATGGGCTAG 18780

TGATTTAATT TACATATATA TATTGGTGTT CTGGGCTCTT ACATGAGAAG ATCTAGTTAA 1884 0

CTGTTGTTAC TGAACAGCGA AGACAAATAT ATAATTTAAG CTCCCCAACT GCTAGTGATT 18900

CTGTTAAGAG GTAATGTTTA AAGTAAATTT ACAAGAGCCC GTCTAGCTCA GTCGGTAGAG 18 960

CGCAAGGCTC TTAACCTTGT GGTCGTGGGT TCGAGCCCCA CGGTGGGCGC ACAATTTTTT 19020

GTTTTTTGAC ATTTTTTGTT TGCTTAGTTG CAGACGGTTT TTCCCCTGCT AGGAGATTTC 19080

CGAGAGAAAA AAAAGGCACT ACAGGTTAAC CAAAACCACC AACCTTTGGA GCGTCGAGGC 1914 0

GACGGGGCAT TTGCGTAGTT GAAGCTTACA AAGTTGCATA TGAGATGAGT GCCGGACATG 19200

AAGCGGATAA CGTTTTAAAC TGGCAACAAT ATCTAGCTGT TTCAAATTCA GGCGTGGGAA 19260

GCTACGCCTA CGCGCCCTGG ACGGCGTGTA AAGAGCCAGC ATCGGCATCA TTGTCAAACG 19320

ATCGACAAGG CCAAGAAATT CCAAATATAT TATTAATAAA AAAGAAGGCA CCAAATTAGT 19380

TTTTGTTTTT TAGTATGTGT GGCGGAGGAA ATTTTGAGAA CGAACGTATC CAAAGAAGGC 19440

ACAAGACGAT ATAGATTGAC GCGGCTAGAA AGTTGCAGCA AGACAGTGGG TACGGTCTTA 19500

TATATCCTAA TAAATAAAAA ATAAAACTAT AGTGTGTCAA ATGTCAACAA GAGGAGGAGG 19560

CAGCCAAATT AGCAGAGGGA GACAAGTAGA GCACGCCTTA TTAGCTTGCT TATTTATCGT 19620

GGTGGTGTAC TTGTTAATTA CTGGCACGCA TTATCAACAA CGCAGTTCTG GATGTGAATC 19680

TAGACAAACA TTTGTCTAGG TTCCGCACGT ATAGTTTTTT TTCTTTTTTT TTGGGGGGGG 19740

GGGGGAACGG AAGCTGTAAT AAACGGTACT AGGAACGAAA GCAACCGCCG CGCGCATGTT 19800

TTTGCAATAG ATTACGGTGA CCTTGATGCA CCACCGCGTG CTATAAAAAC CAGTGTCCCC 198 60

GAGTCTACTC ATCAACCAAT CCATAACTCG AAACCTTTTC TTGTGCTCTG TTCTGTCTGT 19920

GTGTTTCCAA AGCAAGCGAA AGAGGTCGAG GGGATCAGCT TCAAGTTTGT ACAAAAAAGC 19980

AGGCTCCGCG GCCGCCCCCT TCACCATGAC GATGGCTCGT CCTGGGGCGG CTTTGCCGCT 2004 0

GCTGCTGGTC GTGGTCGGCG CTTGCTGCGC GCGCCTGGCG GCGGCAGTGC ACCTCTCCGC 20100

GCTCGGCAGG ACACTCATCG TCGAGGCGTC GCCGAAGGCC GGACAAGTCC TGCACGCCGG 20160

CGAGGACACG ATAACCGTGA CATGGCACCT CAACGCGTCG GCGTCCAGCG TCGGGTACAA 20220

GGCGCTGGAG GTGACCCTCT GCTACGCGCC GGCGAGCCAG GAGGACCGCG GGTGGCGCAA 20280

GGCCAACGAC GACTTGAGCA AGGACAAGGC GTGCCAGTTC AGGATCGCCC GGCATGCATA 2034 0

CGCCGGCGGC CAGGGGACGC TCCGGTACAG GGTCGCCCGC GACGTCCCCA CCGCGTCCTA 204 00

CCACGTGCGC GCCTACGCGC TGGACGCGTC CGGGGCGCCG GTGGGCTACG GCCAGACCGC 204 60

GCCCGCCTAC TACTTCCACG TCGCGGGCGT CTCGGGCGTC CACGCGTCCC TCCGGGTCGC 20520

CGCCGCCGTG CTCTCCGCGT TCTCCATCGC CGCGCTCGCC TTCTTTGTCG TCGTCGAGAA 20580

GAGGAGGAAG GACGAGTAGA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGC 20640

CGTTAACGGA TCCAGACTTG TCCATCTTCT GGATTGGCCA ACTTAATTAA TGTATGAAAT 20700

AAAAGGATGC ACACATAGTG ACATGCTAAT CACTATAATG TGGGCATCAA AGTTGTGTGT 207 60

TATGTGTAAT TACTAGTTAT CTGAATAAAA GAGAAAGAGA TCATCCATAT TTCTTATCCT 20820

AAATGAATGT CACGTGTCTT TATAATTCTT TGATGAACCA GATGCATTTC ATTAACCAAA 20880

TCCATATACA ΤΑΤΑΑΑΤΑΤΤ AATCATATAT AATTAATATC AATTGGGTTA GCAAAACAAA 20940

TCTAGTCTAG GTGTGTTTTG CGAATTGCGG CAAGCTTGCG GCCGCCCCGG GCAACTTTAT 21000

TATACAAAGT TGATAGATAT CGGACCGATT AAACTTTAAT TCGGTCCGAA GCTTGCATGC 21060

CTGCAGTGCA GCGTGACCCG GTCGTGCCCC TCTCTAGAGA TAATGAGCAT TGCATGTCTA 21120

AGTTATAAAA AATTACCACA ΤΑΤΤΤΤΤΤΤΤ GTCACACTTG TTTGAAGTGC AGTTTATCTA 21180

TCTTTATACA TATATTTAAA CTTTACTCTA CGAATAATAT AATCTATAGT ACTACAATAA 2124 0

TATCAGTGTT TTAGAGAATC ATATAAATGA ACAGTTAGAC ATGGTCTAAA GGACAATTGA 21300

GTATTTTGAC AACAGGACTC TACAGTTTTA TCTTTTTAGT GTGCATGTGT TCTCCTTTTT 21360

TTTTGCAAAT AGCTTCACCT ATATAATACT TCATCCATTT TATTAGTACA TCCATTTAGG 21420

GTTTAGGGTT AATGGTTTTT ATAGACTAAT TTTTTTAGTA CATCTATTTT ATTCTATTTT 21480

AGCCTCTAAA TTAAGAAAAC TAAAACTCTA TTTTAGTTTT ΤΤΤΑΤΤΤΑΑΤ AATTTAGATA 21540

TAAAATAGAA TAAAATAAAG TGACTAAAAA TTAAACAAAT ACCCTTTAAG AAATTAAAAA 21600

AACTAAGGAA ACATTTTTCT TGTTTCGAGT AGATAATGCC AGCCTGTTAA ACGCCGTCGA 21660

CGAGTCTAAC GGACACCAAC CAGCGAACCA GCAGCGTCGC GTCGGGCCAA GCGAAGCAGA 21720

CGGCACGGCA TCTCTGTCGC TGCCTCTGGA CCCCTCTCGA GAGTTCCGCT CCACCGTTGG 21780

ACTTGCTCCG CTGTCGGCAT CCAGAAATTG CGTGGCGGAG CGGCAGACGT GAGCCGGCAC 2184 0

GGCAGGCGGC CTCCTCCTCC TCTCACGGCA CCGGCAGCTA CGGGGGATTC CTTTCCCACC 21900GCTCCTTCGC TTTCCCTTCC TCGCCCGCCG TAATAAATAG ACACCCCCTC CACACCCTCT 21960

TTCCCCAACC TCGTGTTGTT CGGAGCGCAC ACACACACAA CCAGATCTCC CCCAAATCCA 22020

CCCGTCGGCA CCTCCGCTTC AAGGTACGCC GCTCGTCCTC CCCCCCCCCC CTCTCTACCT 22080

TCTCTAGATC GGCGTTCCGG TCCATGCATG GTTAGGGCCC GGTAGTTCTA CTTCTGTTCA 22140

TGTTTGTGTT AGATCCGTGT TTGTGTTAGA TCCGTGCTGC TAGCGTTCGT ACACGGATGC 22200

GACCTGTACG TCAGACACGT TCTGATTGCT AACTTGCCAG TGTTTCTCTT TGGGGAATCC 22260

TGGGATGGCT CTAGCCGTTC CGCAGACGGG ATCGATTTCA TGATTTTTTT TGTTTCGTTG 22320

CATAGGGTTT GGTTTGCCCT TTTCCTTTAT TTCAATATAT GCCGTGCACT TGTTTGTCGG 22380

GTCATCTTTT CATGCTTTTT TTTGTCTTGG TTGTGATGAT GTGGTCTGGT TGGGCGGTCG 22440

TTCTAGATCG GAGTAGAATT CTGTTTCAAA CTACCTGGTG GATTTATTAA TTTTGGATCT 22500

GTATGTGTGT GCCATACATA TTCATAGTTA CGAATTGAAG ATGATGGATG GAAATATCGA 22560

TCTAGGATAG GTATACATGT TGATGCGGGT TTTACTGATG CATATACAGA GATGCTTTTT 22620

GTTCGCTTGG TTGTGATGAT GTGGTGTGGT TGGGCGGTCG TTCATTCGTT CTAGATCGGA 22680

GTAGAATACT GTTTCAAACT ACCTGGTGTA TTTATTAATT TTGGAACTGT ATGTGTGTGT 227 40

CATACATCTT CATAGTTACG AGTTTAAGAT GGATGGAAAT ATCGATCTAG GATAGGTATA 22800

CATGTTGATG TGGGTTTTAC TGATGCATAT ACATGATGGC ATATGCAGCA TCTATTCATA 22860

TGCTCTAACC TTGAGTACCT ATCTATTATA ATAAACAAGT ATGTTTTATA ATTATTTTGA 22920

TCTTGATATA CTTGGATGAT GGCATATGCA GCAGCTATAT GTGGATTTTT TTAGCCCTGC 22980

CTTCATACGC TATTTATTTG CTTGGTACTG TTTCTTTTGT CGATGCTCAC CCTGTTGTTT 23040

GGTGTTACTT CTGCAGGTCG ACTTTAACTT AGCCTAGGAT CCACACGACA CCATGTCCCC 23100

CGAGCGCCGC CCCGTCGAGA TCCGCCCGGC CACCGCCGCC GACATGGCCG CCGTGTGCGA 23160

CATCGTGAAC CACTACATCG AGACCTCCAC CGTGAACTTC CGCACCGAGC CGCAGACCCC 23220

GCAGGAGTGG ATCGACGACC TGGAGCGCCT CCAGGACCGC TACCCGTGGC TCGTGGCCGA 23280

GGTGGAGGGC GTGGTGGCCG GCATCGCCTA CGCCGGCCCG TGGAAGGCCC GCAACGCCTA 23340

CGACTGGACC GTGGAGTCCA CCGTGTACGT GTCCCACCGC CACCAGCGCC TCGGCCTCGG 234 00

CTCCACCCTC TACACCCACC TCCTCAAGAG CATGGAGGCC CAGGGCTTCA AGTCCGTGGT 234 60

GGCCGTGATC GGCCTCCCGA ACGACCCGTC CGTGCGCCTC CACGAGGCCC TCGGCTACAC 23520

CGCCCGCGGC ACCCTCCGCG CCGCCGGCTA CAAGCACGGC GGCTGGCACG ACGTCGGCTT 23580

CTGGCAGCGC GACTTCGAGC TGCCGGCCCC GCCGCGCCCG GTGCGCCCGG TGACGCAGAT 23640

CTGAGTCGAA ACCTAGACTT GTCCATCTTC TGGATTGGCC AACTTAATTA ATGTATGAAA 23700

TAAAAGGATG CACACATAGT GACATGCTAA TCACTATAAT GTGGGCATCA AAGTTGTGTG 237 60

TTATGTGTAA TTACTAGTTA TCTGAATAAA AGAGAAAGAG ATCATCCATA TTTCTTATCC 23820

TAAATGAATG TCACGTGTCT TTATAATTCT TTGATGAACC AGATGCATTT CATTAACCAA 23880

ATCCATATAC ATATAAATAT TAATCATATA TAATTAATAT CAATTGGGTT AGCAAAACAA 23940

ATCTAGTCTA GGTGTGTTTT GCGAATGCGG CCGCCACCGC GGTGGAGCTC GAATTCATTC 24 000

CGATTAATCG TGGCCTCTTG CTCTTCAGGA TGAAGAGCTA TGTTTAAACG TGCAAGCGCT 24060

ACTAGACAAT TCAGTACATT AAAAACGTCC GCAATGTGTT ATTAAGTTGT CTAAGCGTCA 24120

ATTTGTTTAC ACCACAATAT ATCCTGCCAC CAGCCAGCCA ACAGCTCCCC GACCGGCAGC 24180

TCGGCACAAA ATCACCACTC GATACAGGCA GCCCATCAGT CCGGGACGGC GTCAGCGGGA 24240

GAGCCGTTGT AAGGCGGCAG ACTTTGCTCA TGTTACCGAT GCTATTCGGA AGAACGGCAA 24 300

CTAAGCTGCC GGGTTTGAAA CACGGATGAT CTCGCGGAGG GTAGCATGTT GATTGTAACG 24 360

ATGACAGAGC GTTGCTGCCT GTGATCAAAT ATCATCTCCC TCGCAGAGAT CCGAATTATC 24420

AGCCTTCTTA TTCATTTCTC GCTTAACCGT GACAGGCTGT CGATCTTGAG AACTATGCCG 24 480

ACATAATAGG AAATCGCTGG ATAAAGCCGC TGAGGAAGCT GAGTGGCGCT ATTTCTTTAG 24 540

AAGTGAACGT TGACGATCGT CGACCGTACC CCGATGAATT AATTCGGACG TACGTTCTGA 24 600

ACACAGCTGG ATACTTACTT GGGCGATTGT CATACATGAC ATCAACAATG TACCCGTTTG 24 660

TGTAACCGTC TCTTGGAGGT TCGTATGACA CTAGTGGTTC CCCTCAGCTT GCGACTAGAT 24 720

GTTGAGGCCT AACATTTTAT TAGAGAGCAG GCTAGTTGCT TAGATACATG ATCTTCAGGC 24 780

CGTTATCTGT CAGGGCAAGC GAAAATTGGC CATTTATGAC GACCAATGCC CCGCAGAAGC 24840

TCCCATCTTT GCCGCCATAG ACGCCGCGCC CCCCTTTTGG GGTGTAGAAC ATCCTTTTGC 24 900

CAGATGTGGA AAAGAAGTTC GTTGTCCCAT TGTTGGCAAT GACGTAGTAG CCGGCGAAAG 24960

TGCGAGACCC ATTTGCGCTA TATATAAGCC TACGATTTCC GTTGCGACTA TTGTCGTAAT 25020

TGGATGAACT ATTATCGTAG TTGCTCTCAG AGTTGTCGTA ATTTGATGGA CTATTGTCGT 25080

AATTGCTTAT GGAGTTGTCG TAGTTGCTTG GAGAAATGTC GTAGTTGGAT GGGGAGTAGT 25140

CATAGGGAAG ACGAGCTTCA TCCACTAAAA CAATTGGCAG GTCAGCAAGT GCCTGCCCCG 25200

ATGCCATCGC AAGTACGAGG CTTAGAACCA CCTTCAACAG ATCGCGCATA GTCTTCCCCA 252 60

GCTCTCTAAC GCTTGAGTTA AGCCGCGCCG CGAAGCGGCG TCGGCTTGAA CGAATTGTTA 25320

GACATTATTT GCCGACTACC TTGGTGATCT CGCCTTTCAC GTAGTGAACA AATTCTTCCA 25380

ACTGATCTGC GCGCGAGGCC AAGCGATCTT CTTGTCCAAG ATAAGCCTGC CTAGCTTCAA 25440GTATGACGGG CTGATACTGG GCCGGCAGGC GCTCCATTGC CCAGTCGGCA GCGACATCCT 25500

TCGGCGCGAT TTTGCCGGTT ACTGCGCTGT ACCAAATGCG GGACAACGTA AGCACTACAT 25560

TTCGCTCATC GCCAGCCCAG TCGGGCGGCG AGTTCCATAG CGTTAAGGTT TCATTTAGCG 25620

CCTCAAATAG ATCCTGTTCA GGAACCGGAT CAAAGAGTTC CTCCGCCGCT GGACCTACCA 25680

AGGCAACGCT ATGTTCTCTT GCTTTTGTCA GCAAGATAGC CAGATCAATG TCGATCGTGG 25740

CTGGCTCGAA GATACCTGCA AGAATGTCAT TGCGCTGCCA TTCTCCAAAT TGCAGTTCGC 25800

GCTTAGCTGG ATAACGCCAC GGAATGATGT CGTCGTGCAC AACAATGGTG ACTTCTACAG 258 60

CGCGGAGAAT CTCGCTCTCT CCAGGGGAAG CCGAAGTTTC CAAAAGGTCG TTGATCAAAG 25920

CTCGCCGCGT TGTTTCATCA AGCCTTACAG TCACCGTAAC CAGCAAATCA ATATCACTGT 25980

GTGGCTTCAG GCCGCCATCC ACTGCGGAGC CGTACAAATG TACGGCCAGC AACGTCGGTT 2604 0

CGAGATGGCG CTCGATGACG CCAACTACCT CTGATAGTTG AGTCGATACT TCGGCGATCA 2 6100

CCGCTTCCCT CATGATGTTT AACTCCTGAA TTAAGCCGCG CCGCGAAGCG GTGTCGGCTT 2 6160

GAATGAATTG TTAGGCGTCA TCCTGTGCTC CCGAGAACCA GTACCAGTAC ATCGCTGTTT 2 6220

CGTTCGAGAC TTGAGGTCTA GTTTTATACG TGAACAGGTC AATGCCGCCG AGAGTAAAGC 26280

CACATTTTGC GTACAAATTG CAGGCAGGTA CATTGTTCGT TTGTGTCTCT AATCGTATGC 2 634 0

CAAGGAGCTG TCTGCTTAGT GCCCACTTTT TCGCAAATTC GATGAGACTG TGCGCGACTC 26400

CTTTGCCTCG GTGCGTGTGC GACACAACAA TGTGTTCGAT AGAGGCTAGA TCGTTCCATG 2 64 60

TTGAGTTGAG TTCAATCTTC CCGACAAGCT CTTGGTCGAT GAATGCGCCA TAGCAAGCAG 2 6520

AGTCTTCATC AGAGTCATCA TCCGAGATGT AATCCTTCCG GTAGGGGCTC ACACTTCTGG 2 6580

TAGATAGTTC AAAGCCTTGG TCGGATAGGT GCACATCGAA CACTTCACGA ACAATGAAAT 2 6640

GGTTCTCAGC ATCCAATGTT TCCGCCACCT GCTCAGGGAT CACCGAAATC TTCATATGAC 2 6700

GCCTAACGCC TGGCACAGCG GATCGCAAAC CTGGCGCGGC TTTTGGCACA AAAGGCGTGA 2 67 60

CAGGTTTGCG AATCCGTTGC TGCCACTTGT TAACCCTTTT GCCAGATTTG GTAACTATAA 2 6820

TTTATGTTAG AGGCGAAGTC TTGGGTAAAA ACTGGCCTAA AATTGCTGGG GATTTCAGGA 26880

AAGTAAACAT CACCTTCCGG CTCGATGTCT ATTGTAGATA TATGTAGTGT ATCTACTTGA 26940

TCGGGGGATC TGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA 27 000

GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 27060

GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA 27120

TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 27180

CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT 2724 0

TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA 27300

GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 27360

ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 27 4 20

TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 274 80

CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 2754 0

TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 27 600

GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 27660

AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 27720

TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 277 80

AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 27840

AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 27 900

TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 27 960

TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 28020

ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 28080

ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 28140

TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 28200

GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 28260

TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 28320

GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 28380

CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 284 4 0

GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTGCAGGG 28500

GGGGGGGGGG GGGGGGACTT CCATTGTTCA TTCCACGGAC AAAAACAGAG AAAGGAAACG 28560

ACAGAGGCCA AAAAGCCTCG CTTTCAGCAC CTGTCGTTTC CTTTCTTTTC AGAGGGTATT 28 620

TTAAATAAAA ACATTAAGTT ATGACGAAGA AGAACGGAAA CGCCTTAAAC CGGAAAATTT 28 680

TCATAAATAG CGAAAACCCG CGAGGTCGCC GCCCCGTAAG CCGCCCCGTA ACCTGTCGGA 2874 0

TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT ACATATCACA ACGTGCGTGG 28800

AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG TATCGTATTA ATTGATCTGC 288 60

ATCAACTTAA CGTAAAAACA ACTTCAGACA ATACAAATCA GCGACACTGA ATACGGGGCA 28920

ACCTCATGTC CCCCCCCCCC CCCCCCCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 28 980TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 2904 0

GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 29100

AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 29160

AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 29220

GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 2 9280

TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 2934 0

GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 294 00

TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 294 60

AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 29520

CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 29580

ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 29640

TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAAGA 297 00

ATTCGGAGCT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG 297 60

ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA 29820

TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT 29880

CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC 2994 0

AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT 30000

GGCAGAGCAT TACGCTGACT TGACGGGACG GCGGCTTTGT TGAATAAATC GAACTTTTGC 30060

TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA 30120

AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT 30180

CACTTTCTGG CTGGATGATG GGGCGATTCA GGCCTGGTAT GAGTCAGCAA CACCTTCTTC 30240

ACGAGGCAGA CCTCAGCGCC AGAAGGCCGC CAGAGAGGCC GAGCGCGGCC GTGAGGCTTG 30300

GACGCTAGGG CAGGGCATGA AAAAGCCCGT AGCGGGCTGC TACGGGCGTC TGACGCGGTG 30360

GAAAGGGGGA GGGGATGTTG TCTACATGGC TCTGCTGTAG TGAGTGGGTT GCGCTCCGGC 30420

AGCGGTCCTG ATCAATCGTC ACCCTTTCTC GGTCCTTCAA CGTTCCTGAC AACGAGCCTC 304 80

CTTTTCGCCA ATCCATCGAC AATCACCGCG AGTCCCTGCT CGAACGCTGC GTCCGGACCG 3054 0

GCTTCGTCGA AGGCGTCTAT CGCGGCCCGC AACAGCGGCG AGAGCGGAGC CTGTTCAACG 30600

GTGCCGCCGC GCTCGCCGGC ATCGCTGTCG CCGGCCTGCT CCTCAAGCAC GGCCCCAACA 30660

GTGAAGTAGC TGATTGTCAT CAGCGCATTG ACGGCGTCCC CGGCCGAAAA ACCCGCCTCG 30720

CAGAGGAAGC GAAGCTGCGC GTCGGCCGTT TCCATCTGCG GTGCGCCCGG TCGCGTGCCG 30780

GCATGGATGC GCGCGCCATC GCGGTAGGCG AGCAGCGCCT GCCTGAAGCT GCGGGCATTC 30840

CCGATCAGAA ATGAGCGCCA GTCGTCGTCG GCTCTCGGCA CCGAATGCGT ATGATTCTCC 30900

GCCAGCATGG CTTCGGCCAG TGCGTCGAGC AGCGCCCGCT TGTTCCTGAA GTGCCAGTAA 30960

AGCGCCGGCT GCTGAACCCC CAACCGTTCC GCCAGTTTGC GTGTCGTCAG ACCGTCTACG 31020

CCGACCTCGT TCAACAGGTC CAGGGCGGCA CGGATCACTG TATTCGGCTG CAACTTTGTC 31080

ATGCTTGACA CTTTATCACT GATAAACATA ATATGTCCAC CAACTTATCA GTGATAAAGA 3114 0

ATCCGCGCGT TCAATCGGAC CAGCGGAGGC TGGTCCGGAG GCCAGACGTG AAACCCAACA 31200

TACCCCTGAT CGTAATTCTG AGCACTGTCG CGCTCGACGC TGTCGGCATC GGCCTGATTA 312 60

TGCCGGTGCT GCCGGGCCTC CTGCGCGATC TGGTTCACTC GAACGACGTC ACCGCCCACT 31320

ATGGCATTCT GCTGGCGCTG TATGCGTTGG TGCAATTTGC CTGCGCACCT GTGCTGGGCG 31380

CGCTGTCGGA TCGTTTCGGG CGGCGGCCAA TCTTGCTCGT CTCGCTGGCC GGCGCCACTG 31440

TCGACTACGC CATCATGGCG ACAGCGCCTT TCCTTTGGGT TCTCTATATC GGGCGGATCG 31500

TGGCCGGCAT CACCGGGGCG ACTGGGGCGG TAGCCGGCGC TTATATTGCC GATATCACTG 31560

ATGGCGATGA GCGCGCGCGG CACTTCGGCT TCATGAGCGC CTGTTTCGGG TTCGGGATGG 31620

TCGCGGGACC TGTGCTCGGT GGGCTGATGG GCGGTTTCTC CCCCCACGCT CCGTTCTTCG 31680

CCGCGGCAGC CTTGAACGGC CTCAATTTCC TGACGGGCTG TTTCCTTTTG CCGGAGTCGC 3174 0

ACAAAGGCGA ACGCCGGCCG TTACGCCGGG AGGCTCTCAA CCCGCTCGCT TCGTTCCGGT 31800

GGGCCCGGGG CATGACCGTC GTCGCCGCCC TGATGGCGGT CTTCTTCATC ATGCAACTTG 31860

TCGGACAGGT GCCGGCCGCG CTTTGGGTCA TTTTCGGCGA GGATCGCTTT CACTGGGACG 31920

CGACCACGAT CGGCATTTCG CTTGCCGCAT TTGGCATTCT GCATTCACTC GCCCAGGCAA 31980

TGATCACCGG CCCTGTAGCC GCCCGGCTCG GCGAAAGGCG GGCACTCATG CTCGGAATGA 32040

TTGCCGACGG CACAGGCTAC ATCCTGCTTG CCTTCGCGAC ACGGGGATGG ATGGCGTTCC 32100

CGATCATGGT CCTGCTTGCT TCGGGTGGCA TCGGAATGCC GGCGCTGCAA GCAATGTTGT 32160

CCAGGCAGGT GGATGAGGAA CGTCAGGGGC AGCTGCAAGG CTCACTGGCG GCGCTCACCA 32220

GCCTGACCTC GATCGTCGGA CCCCTCCTCT TCACGGCGAT CTATGCGGCT TCTATAACAA 32280

CGTGGAACGG GTGGGCATGG ATTGCAGGCG CTGCCCTCTA CTTGCTCTGC CTGCCGGCGC 32340

TGCGTCGCGG GCTTTGGAGC GGCGCAGGGC AACGAGCCGA TCGCTGATCG TGGAAACGAT 324 00

AGGCCTATGC CATGCGGGTC AAGGCGACTT CCGGCAAGCT ATACGCGCCC TAGGAGTGCG 324 60

GTTGGAACGT TGGCCCAGCC AGATACTCCC GATCACGAGC AGGACGCCGA TGATTTGAAG 32520CGCACTCAGC GTCTGATCCA AGAACAACCA TCCTAGCAAC ACGGCGGTCC CCGGGCTGAG 32580

AAAGCCCAGT AAGGAAACAA CTGTAGGTTC GAGTCGCGAG ATCCCCCGGA ACCAAAGGAA 3264 0

GTAGGTTAAA CCCGCTCCGA TCAGGCCGAG CCACGCCAGG CCGAGAACAT TGGTTCCTGT 327 00

AGGCATCGGG ATTGGCGGAT CAAACACTAA AGCTACTGGA ACGAGCAGAA GTCCTCCGGC 32760

CGCCAGTTGC CAGGCGGTAA AGGTGAGCAG AGGCACGGGA GGTTGCCACT TGCGGGTCAG 32820

CACGGTTCCG AACGCCATGG AAACCGCCCC CGCCAGGCCC GCTGCGACGC CGACAGGATC 32880

TAGCGCTGCG TTTGGTGTCA ACACCAACAG CGCCACGCCC GCAGTTCCGC AAATAGCCCC 3294 0

CAGGACCGCC ATCAATCGTA TCGGGCTACC TAGCAGAGCG GCAGAGATGA ACACGACCAT 33000

CAGCGGCTGC ACAGCGCCTA CCGTCGCCGC GACCCCGCCC GGCAGGCGGT AGACCGAAAT 33060

AAACAACAAG CTCCAGAATA GCGAAATATT AAGTGCGCCG AGGATGAAGA TGCGCATCCA 33120

CCAGATTCCC GTTGGAATCT GTCGGACGAT CATCACGAGC AATAAACCCG CCGGCAACGC 33180

CCGCAGCAGC ATACCGGCGA CCCCTCGGCC TCGCTGTTCG GGCTCCACGA AAACGCCGGA 3324 0

CAGATGCGCC TTGTGAGCGT CCTTGGGGCC GTCCTCCTGT TTGAAGACCG ACAGCCCAAT 33300

GATCTCGCCG TCGATGTAGG CGCCGAATGC CACGGCATCT CGCAACCGTT CAGCGAACGC 33360

CTCCATGGGC TTTTTCTCCT CGTGCTCGTA AACGGACCCG AACATCTCTG GAGCTTTCTT 33420

CAGGGCCGAC AATCGGATCT CGCGGAAATC CTGCACGTCG GCCGCTCCAA GCCGTCGAAT 33480

CTGAGCCTTA ATCACAATTG TCAATTTTAA TCCTCTGTTT ATCGGCAGTT CGTAGAGCGC 33540

GCCGTGCGTC CCGAGCGATA CTGAGCGAAG CAAGTGCGTC GAGCAGTGCC CGCTTGTTCC 33600

TGAAATGCCA GTAAAGCGCT GGCTGCTGAA CCCCCAGCCG GAACTGACCC CACAAGGCCC 33660

TAGCGTTTGC AATGCACCAG GTCATCATTG ACCCAGGCGT GTTCCACCAG GCCGCTGCCT 33720

CGCAACTCTT CGCAGGCTTC GCCGACCTGC TCGCGCCACT TCTTCACGCG GGTGGAATCC 33780

GATCCGCACA TGAGGCGGAA GGTTTCCAGC TTGAGCGGGT ACGGCTCCCG GTGCGAGCTG 338 4 0

AAATAGTCGA ACATCCGTCG GGCCGTCGGC GACAGCTTGC GGTACTTCTC CCATATGAAT 33900

TTCGTGTAGT GGTCGCCAGC AAACAGCACG ACGATTTCCT CGTCGATCAG GACCTGGCAA 33960

CGGGACGTTT TCTTGCCACG GTCCAGGACG CGGAAGCGGT GCAGCAGCGA CACCGATTCC 34 020

AGGTGCCCAA CGCGGTCGGA CGTGAAGCCC ATCGCCGTCG CCTGTAGGCG CGACAGGCAT 34 080

TCCTCGGCCT TCGTGTAATA CCGGCCATTG ATCGACCAGC CCAGGTCCTG GCAAAGCTCG 3414 0

TAGAACGTGA AGGTGATCGG CTCGCCGATA GGGGTGCGCT TCGCGTACTC CAACACCTGC 34 200

TGCCACACCA GTTCGTCATC GTCGGCCCGC AGCTCGACGC CGGTGTAGGT GATCTTCACG 34 2 60

TCCTTGTTGA CGTGGAAAAT GACCTTGTTT TGCAGCGCCT CGCGCGGGAT TTTCTTGTTG 34 320

CGCGTGGTGA ACAGGGCAGA GCGGGCCGTG TCGTTTGGCA TCGCTCGCAT CGTGTCCGGC 34 380

CACGGCGCAA TATCGAACAA GGAAAGCTGC ATTTCCTTGA TCTGCTGCTT CGTGTGTTTC 344 40

AGCAACGCGG CCTGCTTGGC CTCGCTGACC TGTTTTGCCA GGTCCTCGCC GGCGGTTTTT 34 500

CGCTTCTTGG TCGTCATAGT TCCTCGCGTG TCGATGGTCA TCGACTTCGC CAAACCTGCC 34560

GCCTCCTGTT CGAGACGACG CGAACGCTCC ACGGCGGCCG ATGGCGCGGG CAGGGCAGGG 34 620

GGAGCCAGTT GCACGCTGTC GCGCTCGATC TTGGCCGTAG CTTGCTGGAC CATCGAGCCG 34 680

ACGGACTGGA AGGTTTCGCG GGGCGCACGC ATGACGGTGC GGCTTGCGAT GGTTTCGGCA 34 740

TCCTCGGCGG AAAACCCCGC GTCGATCAGT TCTTGCCTGT ATGCCTTCCG GTCAAACGTC 34 800

CGATTCATTC ACCCTCCTTG CGGGATTGCC CCGACTCACG CCGGGGCAAT GTGCCCTTAT 34 860

TCCTGATTTG ACCCGCCTGG TGCCTTGGTG TCCAGATAAT CCACCTTATC GGCAATGAAG 34 920

TCGGTCCCGT AGACCGTCTG GCCGTCCTTC TCGTACTTGG TATTCCGAAT CTTGCCCTGC 34 980

ACGAATACCA GCGACCCCTT GCCCAAATAC TTGCCGTGGG CCTCGGCCTG AGAGCCAAAA 3504 0

CACTTGATGC GGAAGAAGTC GGTGCGCTCC TGCTTGTCGC CGGCATCGTT GCGCCACTCT 35100

TCATTAACCG CTATATCGAA AATTGCTTGC GGCTTGTTAG AATTGCCATG ACGTACCTCG 35160

GTGTCACGGG TAAGATTACC GATAAACTGG AACTGATTAT GGCTCATATC GAAAGTCTCC 35220

TTGAGAAAGG AGACTCTAGT TTAGCTAAAC ATTGGTTCCG CTGTCAAGAA CTTTAGCGGC 35280

TAAAATTTTG CGGGCCGCGA CCAAAGGTGC GAGGGGCGGC TTCCGCTGTG TACAACCAGA 35340

TATTTTTCAC CAACATCCTT CGTCTGCTCG ATGAGCGGGG CATGACGAAA CATGAGCTGT 354 00

CGGAGAGGGC AGGGGTTTCA ATTTCGTTTT TATCAGACTT AACCAACGGT AAGGCCAACC 354 60

CCTCGTTGAA GGTGATGGAG GCCATTGCCG ACGCCCTGGA AACTCCCCTA CCTCTTCTCC 35520

TGGAGTCCAC CGACCTTGAC CGCGAGGCAC TCGCGGAGAT TGCGGGTCAT CCTTTCAAGA 35580

GCAGCGTGCC GCCCGGATAC GAACGCATCA GTGTGGTTTT GCCGTCACAT AAGGCGTTTA 3564 0

TCGTAAAGAA ATGGGGCGAC GACACCCGAA AAAAGCTGCG TGGAAGGCTC TGACGCCAAG 35700

GGTTAGGGCT TGCACTTCCT TCTTTAGCCG CTAAAACGGC CCCTTCTCTG CGGGCCGTCG 35760

GCTCGCGCAT CATATCGACA TCCTCAACGG AAGCCGTGCC GCGAATGGCA TCGGGCGGGT 35820

GCGCTTTGAC AGTTGTTTTC TATCAGAACC CCTACGTCGT GCGGTTCGAT TAGCTGTTTG 35880

TCTTGCAGGC TAAACACTTT CGGTATATCG TTTGCCTGTG CGATAATGTT GCTAATGATT 3594 0

TGTTGCGTAG GGGTTACTGA AAAGTGAGCG GGAAAGAAGA GTTTCAGACC ATCAAGGAGC 36000

GGGCCAAGCG CAAGCTGGAA CGCGACATGG GTGCGGACCT GTTGGCCGCG CTCAACGACC 36060CGAAAACCGT TGAAGTCATG CTCAACGCGGAGCCGATGCG GTACATCTGC GACATGCGGCTGGCCGGATT CCACGGCAAA GAGGTCACGCCCTTGGATGG CAGCCGCTTT GCCGGCCAATCGATCCGCAA GCGCGCGGTC GCCATCTTCATGACCCGCGA GCAATACGAG GTCATTAAAATCATTGGCGG TACTGGCTCG GGCAAGACCATCGCCTTCAA CCCGTCTGAG CGCGTCGTCACCGCAGAGAA CGCCGTCCAA TACCACACCAAGACAACGCT GCGTATGCGC CCCGACCGCACCCTTGATCT GTTGATGGCC TGGAACACCGCAAACAACCC CAAAGCGGGC CTGAGCCGGCCACCGAAACC CATTGAGCCG CTGATTGGCGGGACCCCTAG CGGCCGTCGA GTGCAAGAAAAGTACATCAC CAAAACCCTG TAAGGAGTATTGACCATGAA TCGCGGCATT TTGTTCTACCTATCCGCGCA TCCGGCGATG GCCTCGGAAGGGCTGACGAA CCTGCGCAAC TCCGTAACCGGCATCGTCGT CGCCGGCGGC GTGCTGATCTCCCTGATCTT CCTGGTTCTG GTGATGGCGCCCTTCTTCGG TCGTGGTGCC GAAATCGCGGAAGTCGCGGC GGCGGATGCC GTGCGTGCGGTCTGCGCACG ATCCCCATCC GTCGCGCAGGTCGTGAACTG GTGATGTTCT CGGGCCTGATGCTGCGGGCC ACCGTGGTCG GTCTGATCCTCATGGCGAAG GCCGATCCGA AGATGCGGTTGTATTACCCG GCCCGCTCGA CCCCGTTCCGCCGATGATCC AAGCAATTGC GATTGCAATCCTCTTTGCCC GCATCCGCGC GGTCGATGCCGACGCCGGCC TGGCCGATCT GCTCAACTACGGCAAGAACG GCAGCTTTAT GGCTGCCTGGACCGACCAGC AGCGCGAAGT AGTGTCCGCCAGTGGGTGGA TGATCCATGT GGACGCCGTGGGCCTGTCGG CGTTCCCTGA CCGTCTGACGCCTTGCTCGT CGGTGATGTA CTTCACCAGCATGGGGACGT GCTTGGCAAT CACGCGCACCTGGCTCTGCC CTCGGGCGGA CCACGCCCATTTCGATCTTC GCCAGCAGGG CGAGGATCGTGTCGGTGAGC CAGAGTTTCA GCAGGCCGCCCAGCTCGCGG ACGTGCTCAT AGTCCACGACCAGCAGGTAG GCCGACAGGC TCATGCCGGCTTCGTCTGGA AGGCAGTACA CCTTGATAGGATCAGCCATC CGCTTGCCCT CATCTGTTACAGGATTCCCG TTGAGCACCG CCAGGTGCGACGCGGGTGGG CCTACTTCAC CTATCCTGCCGTCTACACGA ACCCTTTGGC AAAATCCTGTAAAAATCGCT ATAATGACCC CGAAGCAGGGTGTTTTGTGG AATATCTACC GACTGGAAACGGACAGGCGA GAGACGATGC CAAAGAGCTACCGCGCGGCC AAGAAGCGCC GGCGTGATGAGGATGTCGAG GCGGCGTTAG CGTCCGGCTAGGAAACGGGG AAGGTCAAGT TCTCCTACGACAAGGCCAAG CCCGCCGATG TGCCCGCACCACCCAAGACG CCGGAGCCAC GGCGGCCGAACGCTGCGGCC CCGACCGGCT TCACCTTCAAATGGCGAAAA TTCACATGGT TTTGCAGGGCGCGATCATTG CGCAGTACAA GATGGACAAGCCGGTGAACG CGACGTTCGA GGGCTACAAGGCCGGCGACG AAATTAACTC GCGCAACTTC

ACGGCAAGGT GTGGCACGAA CGCCTTGGCG 36120

CCAGCCAGTC GCAGGCGATT ATAGAAACGG 36180

GGCATTCGCC CATCCTGGAA GGCGAGTTCC 36240

TGCCGCCGGT CGTGGCCGCG CCAACCTTTG 36300

CGCTGGAACA GTACGTCGAG GCGGGCATCA 36360

GCGCCGTCGC GGCGCATCGA AACATCCTCG 36420

CGCTCGTCAA CGCGATCATC AATGAAATGG 36480

TCATCGAGGA CACCGGCGAA ATCCAGTGCG 36540

GCATCGACGT CTCGATGACG CTGCTGCTCA 36600

TCCTGGTCGG TGAGGTACGT GGCCCCGAAG 36660

GGCATGAAGG AGGTGCCGCC ACCCTGCACG 36720

TCGCCATGCT TATCAGCATG CACCCGGATT 36780

AGGCGGTTCA TGTGGTCGTC CATATCGCCA 36840

TTCTCGAAGT TCTTGGTTAC GAGAACGGCC 36900

TTCCAATGAC AACGGCTGTT CCGTTCCGTC 36960

TTGCCGTGTT CTTCGTTCTC GCTCTCGCGT 37020

GCACCGGCGG CAGCTTGCCA TATGAGAGCT 37080

GCCCGGTGGC CTTCGCGCTG TCCATCATCG 37140

TCGGCGGCGA ACTCAACGCC TTCTTCCGAA 37200

TGCTGGTCGG CGCGCAGAAC GTGATGAGCA 37260

CCCTCGGCAA CGGGGCGCTG CACCAGGTGC 37320

TAGCGGCTGG ACGGCTCGCC TAATCATGGC 37 380

CAACCGAGAA AACCTGTTCA TGGGTGGTGA 374 4 0

GGCGTTTGCG CTGATTTTCA GCGCCCAAGA 37500

GTGGTTCGGG GCGCTCTATG CGTTCCGAAT 37560

CGTGTACCTG CGTCACCGCC GGTACAAGCC 37 620

CGAGAACACC AATAGCCAAG GGAAGCAATA 37 680

GCGGGCCTCG GCGCGCTTCT GTTGTTCATC 37740

GAACTGAAAC TGAAAAAGCA TCGTTCCAAG 37 800

GCCGCTGTCG TCGATGACGG CGTAATCGTG 378 60

CTGTACAAGG GCGATGACAA CGCAAGCAGC 37920

CGCATCAACC AGGCCCTCGC GGGCCTGGGA 37 980

CGGCGTCCTG CTCCGAACTA CGCGGAGCGG 3804 0

GCAGCGATTG AAGAAGAGCG CTCGGTCTTG 38100

TCCGCGAAGT CGCTCTTCTT GATGGAGCGC 38160

CCCCGGCCGT TTTAGCGGCT AAAAAAGTCA 38220

CATGACCTTG CCAAGCTCGT CCTGCTTCTC 38280

GGCATCACCG AACCGCGCCG TGCGCGGGTC 3834 0

CAGGCGGCCC AGGTCGCCAT TGATGCGGGC 38400

GCCCGTGATT TTGTAGCCCT GGCCGACGGC 384 60

CGCCGCCGCC TTTTCCTCAA TCGCTCTTCG 38520

TGGGCTGCCC TTCCTGGTTG GCTTGGTTTC 38580

GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC 38 640

ATAAGGGACA GTGAAGAAGG AACACCCGCT 38700

CGGCTGACGC CGTTGGATAC ACCAAGGAAA 387 60

ATATCGTGCG AAAAAGGATG GATATACCGA 38820

TTATGCAGCG GAAAAGCGCT GCTTCCCTGC 38880

AGGCAAATGC AGGAAATTAC TGAACTGAGG 38 94 0

CACCGACGAG CTGGCCGAGT GGGTTGAATC 39000

GGCTGCGGTT GCGTTCCTGG CGGTGAGGGC 39060

TGCGCTCGTC ACCATTTGGG AGCACATGCG 39120

GACGTTCCGC TCGCACGCCA GGCGGCACAT 39180

GCAGGCCAAG GCTGCGGAAC CCGCGCCGGC 39240

GCAGGGGGGC AAGGCTGAAA AGCCGGCCCC 39300

CCCAACACCG GACAAAAAGG ATCTACTGTA 39360

AAGGGCGGGG TCGGCAAGTC GGCCATCGCC 39420

GGGCAGACAC CCTTGTGCAT CGACACCGAC 39480

GCCCTGAACG TCCGCCGGCT GAACATCATG 3954 0

GACACCCTGG TCGAGCTGAT TGCGCCGACC 39600AAGGATGACG TGGTGATCGA CAACGGTGCC AGCTCGTTCG TGCCTCTGTC GCATTACCTC 39660

ATCAGCAACC AGGTGCCGGC TCTGCTGCAA GAAATGGGGC ATGAGCTGGT CATCCATACC 39720

GTCGTCACCG GCGGCCAGGC TCTCCTGGAC ACGGTGAGCG GCTTCGCCCA GCTCGCCAGC 39780

CAGTTCCCGG CCGAAGCGCT TTTCGTGGTC TGGCTGAACC CGTATTGGGG GCCTATCGAG 39840

CATGAGGGCA AGAGCTTTGA GCAGATGAAG GCGTACACGG CCAACAAGGC CCGCGTGTCG 39900

TCCATCATCC AGATTCCGGC CCTCAAGGAA GAAACCTACG GCCGCGATTT CAGCGACATG 39960

CTGCAAGAGC GGCTGACGTT CGACCAGGCG CTGGCCGATG AATCGCTCAC GATCATGACG 4 0020

CGGCAACGCC TCAAGATCGT GCGGCGCGGC CTGTTTGAAC AGCTCGACGC GGCGGCCGTG 4 0080

CTATGAGCGA CCAGATTGAA GAGCTGATCC GGGAGATTGC GGCCAAGCAC GGCATCGCCG 4 014 0

TCGGCCGCGA CGACCCGGTG CTGATCCTGC ATACCATCAA CGCCCGGCTC ATGGCCGACA 4 0200

GTGCGGCCAA GCAAGAGGAA ATCCTTGCCG CGTTCAAGGA AGAGCTGGAA GGGATCGCCC 40260

ATCGTTGGGG CGAGGACGCC AAGGCCAAAG CGGAGCGGAT GCTGAACGCG GCCCTGGCGG 4 0320

CCAGCAAGGA CGCAATGGCG AAGGTAATGA AGGACAGCGC CGCGCAGGCG GCCGAAGCGA 4 0380

TCCGCAGGGA AATCGACGAC GGCCTTGGCC GCCAGCTCGC GGCCAAGGTC GCGGACGCGC 4 0440

GGCGCGTGGC GATGATGAAC ATGATCGCCG GCGGCATGGT GTTGTTCGCG GCCGCCCTGG 40500

TGGTGTGGGC CTCGTTATGA ATCGCAGAGG CGCAGATGAA AAAGCCCGGC GTTGCCGGGC 40560

TTTGTTTTTG CGTTAGCTGG GCTTGTTTGA CAGGCCCAAG CTCTGACTGC GCCCGCGCTC 40620

GCGCTCCTGG GCCTGTTTCT TCTCCTGCTC CTGCTTGCGC ATCAGGGCCT GGTGCCGTCG 4 0680

GGCTGCTTCA CGCATCGAAT CCCAGTCGCC GGCCAGCTCG GGATGCTCCG CGCGCATCTT 4 0740

GCGCGTCGCC AGTTCCTCGA TCTTGGGCGC GTGAATGCCC ATGCCTTCCT TGATTTCGCG 4 0800

CACCATGTCC AGCCGCGTGT GCAGGGTCTG CAAGCGGGCT TGCTGTTGGG CCTGCTGCTG 4 08 60

CTGCCAGGCG GCCTTTGTAC GCGGCAGGGA CAGCAAGCCG GGGGCATTGG ACTGTAGCTG 4 0920

CTGCAAACGC GCCTGCTGAC GGTCTACGAG CTGTTCTAGG CGGTCCTCGA TGCGCTCCAC 4 0980

CTGGTCATGC TTTGCCTGCA CGTAGAGCGC AAGGGTCTGC TGGTAGGTCT GCTCGATGGG 4104 0

CGCGGATTCT AAGAGGGCCT GCTGTTCCGT CTCGGCCTCC TGGGCCGCCT GTAGCAAATC 41100

CTCGCCGCTG TTGCCGCTGG ACTGCTTTAC TGCCGGGGAC TGCTGTTGCC CTGCTCGCGC 41160

CGTCGTCGCA GTTCGGCTTG CCCCCACTCG ATTGACTGCT TCATTTCGAG CCGCAGCGAT 41220

GCGATCTCGG ATTGCGTCAA CGGACGGGGC AGCGCGGAGG TGTCCGGCTT CTCCTTGGGT 41280

GAGTCGGTCG ATGCCATAGC CAAAGGTTTC CTTCCAAAAT GCGTCCATTG CTGGACCGTG 41340

TTTCTCATTG ATGCCCGCAA GCATCTTCGG CTTGACCGCC AGGTCAAGCG CGCCTTCATG 414 00

GGCGGTCATG ACGGACGCCG CCATGACCTT GCCGCCGTTG TTCTCGATGT AGCCGCGTAA 414 60

TGAGGCAATG GTGCCGCCCA TCGTCAGCGT GTCATCGACA ACGATGTACT TCTGGCCGGG 41520

GATCACCTCC CCCTCGAAAG TCGGGTTGAA CGCCAGGCGA TGATCTGAAC CGGCTCCGGT 41580

TCGGGCGACC TTCTCCCGCT GCACAATGTC CGTTTCGACC TCAAGGCCAA GGCGGTCGGC 41640

CAGAACGACC GCCATCATGG CCGGAATCTT GTTGTTCCCC GCCGCCTCGA CGGCGAGGAC 41700

TGGAACGATG CGGGGCTTGT CGTCGCCGAT CAGCGTCTTG AGCTGGGCAA CAGTGTCGTC 417 60

CGAAATCAGG CGCTCGACCA AATTAAGCGC CGCTTCCGCG TCGCCCTGCT TCGCAGCCTG 41820

GTATTCAGGC TCGTTGGTCA AAGAACCAAG GTCGCCGTTG CGAACCACCT TCGGGAAGTC 41880

TCCCCACGGT GCGCGCTCGG CTCTGCTGTA GCTGCTCAAG ACGCCTCCCT TTTTAGCCGC 41940

TAAAACTCTA ACGAGTGCGC CCGCGACTCA ACTTGACGCT TTCGGCACTT ACCTGTGCCT 4 2000

TGCCACTTGC GTCATAGGTG ATGCTTTTCG CACTCCCGAT TTCAGGTACT TTATCGAAAT 42060

CTGACCGGGC GTGCATTACA AAGTTCTTCC CCACCTGTTG GTAAATGCTG CCGCTATCTG 42120

CGTGGACGAT GCTGCCGTCG TGGCGCTGCG ACTTATCGGC CTTTTGGGCC ATATAGATGT 42180

TGTAAATGCC AGGTTTCAGG GCCCCGGCTT TATCTACCTT CTGGTTCGTC CATGCGCCTT 4224 0

GGTTCTCGGT CTGGACAATT CTTTGCCCAT TCATGACCAG GAGGCGGTGT TTCATTGGGT 4 2300

GACTCCTGAC GGTTGCCTCT GGTGTTAAAC GTGTCCTGGT CGCTTGCCGG CTAAAAAAAA 4 2360

GCCGACCTCG GCAGTTCGAG GCCGGCTTTC CCTAGAGCCG GGCGCGTCAA GGTTGTTCCA 4 24 20

TCTATTTTAG TGAACTGCGT TCGATTTATC AGTTACTTTC CTCCCGCTTT GTGTTTCCTC 4 2480

CCACTCGTTT CCGCGTCTAG CCGACCCCTC AACATAGCGG CCTCTTCTTG GGCTGCCTTT 4 254 0

GCCTCTTGCC GCGCTTCGTC ACGCTCGGCT TGCACCGTCG TAAAGCGCTC GGCCTGCCTG 4 2 600

GCCGCCTCTT GCGCCGCCAA CTTCCTTTGC TCCTGGTGGG CCTCGGCGTC GGCCTGCGCC 4 2 660

TTCGCTTTCA CCGCTGCCAA CTCCGTGCGC AAACTCTCCG CTTCGCGCCT GGTGGCGTCG 42720

CGCTCGCCGC GAAGCGCCTG CATTTCCTGG TTGGCCGCGT CCAGGGTCTT GCGGCTCTCT 4 27 80

TCTTTGAATG CGCGGGCGTC CTGGTGAGCG TAGTCCAGCT CGGCGCGCAG CTCCTGCGCT 4 2840

CGACGCTCCA CCTCGTCGGC CCGCTGCGTC GCCAGCGCGG CCCGCTGCTC GGCTCCTGCC 4 2900

AGGGCGGTGC GTGCTTCGGC CAGGGCTTGC CGCTGGCGTG CGGCCAGCTC GGCCGCCTCG 42960

GCGGCCTGCT GCTCTAGCAA TGTAACGCGC GCCTGGGCTT CTTCCAGCTC GCGGGCCTGC 4 3020

GCCTCGAAGG CGTCGGCCAG CTCCCCGCGC ACGGCTTCCA ACTCGTTGCG CTCACGATCC 4 3080

CAGCCGGCTT GCGCTGCCTG CAACGATTCA TTGGCAAGGG CCTGGGCGGC TTGCCAGAGG 4 314 0GCGGCCACGG CCTGGTTGCC GGCCTGCTGC ACCGCGTCCG GCACCTGGAC TGCCAGCGGG 43200

GCGGCCTGCG CCGTGCGCTG GCGTCGCCAT TCGCGCATGC CGGCGCTGGC GTCGTTCATG 43260

TTGACGCGGG CGGCCTTACG CACTGCATCC ACGGTCGGGA AGTTCTCCCG GTCGCCTTGC 4 3320

TCGAACAGCT CGTCCGCAGC CGCAAAAATG CGGTCGCGCG TCTCTTTGTT CAGTTCCATG 4 3380

TTGGCTCCGG TAATTGGTAA GAATAATAAT ACTCTTACCT ACCTTATCAG CGCAAGAGTT 4 3440

TAGCTGAACA GTTCTCGACT TAACGGCAGG TTTTTTAGCG GCTGAAGGGC AGGCAAAAAA 43500

AGCCCCGCAC GGTCGGCGGG GGCAAAGGGT CAGCGGGAAG GGGATTAGCG GGCGTCGGGC 43560

TTCTTCATGC GTCGGGGCCG CGCTTCTTGG GATGGAGCAC GACGAAGCGC GCACGCGCAT 4 3620

CGTCCTCGGC CCTATCGGCC CGCGTCGCGG TCAGGAACTT GTCGCGCGCT AGGTCCTCCC 4 3680

TGGTGGGCAC CAGGGGCATG AACTCGGCCT GCTCGATGTA GGTCCACTCC ATGACCGCAT 43740

CGCAGTCGAG GCCGCGTTCC TTCACCGTCT CTTGCAGGTC GCGGTACGCC CGCTCGTTGA 43800

GCGGCTGGTA ACGGGCCAAT TGGTCGTAAA TGGCTGTCGG CCATGAGCGG CCTTTCCTGT 4 3860

TGAGCCAGCA GCCGACGACG AAGCCGGCAA TGCAGGCCCC TGGCACAACC AGGCCGACGC 43920

CGGGGGCAGG GGATGGCAGC AGCTCGCCAA CCAGGAACCC CGCCGCGATG ATGCCGATGC 4 3980

CGGTCAACCA GCCCTTGAAA CTATCCGGCC CCGAAACACC CCTGCGCATT GCCTGGATGC 4 4 040

TGCGCCGGAT AGCTTGCAAC ATCAGGAGCC GTTTCTTTTG TTCGTCAGTC ATGGTCCGCC 44100

CTCACCAGTT GTTCGTATCG GTGTCGGACG AACTGAAATC GCAAGAGCTG CCGGTATCGG 44160

TCCAGCCGCT GTCCGTGTCG CTGCTGCCGA AGCACGGCGA GGGGTCCGCG AACGCCGCAG 4 4 220

ACGGCGTATC CGGCCGCAGC GCATCGCCCA GCATGGCCCC GGTCAGCGAG CCGCCGGCCA 4 4 280

GGTAGCCCAG CATGGTGCTG TTGGTCGCCC CGGCCACCAG GGCCGACGTG ACGAAATCGC 4 4 340

CGTCATTCCC TCTGGATTGT TCGCTGCTCG GCGGGGCAGT GCGCCGCGCC GGCGGCGTCG 44400

TGGATGGCTC GGGTTGGCTG GCCTGCGACG GCCGGCGAAA GGTGCGCAGC AGCTCGTTAT 444 60

CGACCGGCTG CGGCGTCGGG GCCGCCGCCT TGCGCTGCGG TCGGTGTTCC TTCTTCGGCT 4 4 520

CGCGCAGCTT GAACAGCATG ATCGCGGAAA CCAGCAGCAA CGCCGCGCCT ACGCCTCCCG 4 4 580

CGATGTAGAA CAGCATCGGA TTCATTCTTC GGTCCTCCTT GTAGCGGAAC CGTTGTCTGT 44640

GCGGCGCGGG TGGCCCGCGC CGCTGTCTTT GGGGATCAGC CCTCGATGAG CGCGACCAGT 4 4 700

TTCACGTCGG CAAGGTTCGC CTCGAACTCC TGGCCGTCGT CCTCGTACTT CAACCAGGCA 44760

TAGCCTTCCG CCGGCGGCCG ACGGTTGAGG ATAAGGCGGG CAGGGCGCTC GTCGTGCTCG 4 4 820

ACCTGGACGA TGGCCTTTTT CAGCTTGTCC GGGTCCGGCT CCTTCGCGCC CTTTTCCTTG 44880

GCGTCCTTAC CGTCCTGGTC GCCGTCCTCG CCGTCCTGGC CGTCGCCGGC CTCCGCGTCA 4 4 940

CGCTCGGCAT CAGTCTGGCC GTTGAAGGCA TCGACGGTGT TGGGATCGCG GCCCTTCTCG 45000

TCCAGGAACT CGCGCAGCAG CTTGACCGTG CCGCGCGTGA TTTCCTGGGT GTCGTCGTCA 45060

AGCCACGCCT CGACTTCCTC CGGGCGCTTC TTGAAGGCCG TCACCAGCTC GTTCACCACG 45120

GTCACGTCGC GCACGCGGCC GGTGTTGAAC GCATCGGCGA TCTTCTCCGG CAGGTCCAGC 4 5180

AGCGTGACGT GCTGGGTGAT GAACGCCGGC GACTTGCCGA TTTCCTTGGC GATATCGCCT 4 5240

TTCTTCTTGC CCTTCGCCAG CTCGCGGCCA ATGAAGTCGG CAATTTCGCG CGGGGTCAGC 4 5300

TCGTTGCGTT GCAGGTTCTC GATAACCTGG TCGGCTTCGT TGTAGTCGTT GTCGATGAAC 45360

GCCGGGATGG ACTTCTTGCC GGCCCACTTC GAGCCACGGT AGCGGCGGGC GCCGTGATTG 4 5420

ATGATATAGC GGCCCGGCTG CTCCTGGTTC TCGCGCACCG AAATGGGTGA CTTCACCCCG 4 5480

CGCTCTTTGA TCGTGGCACC GATTTCCGCG ATGCTCTCCG GGGAAAAGCC GGGGTTGTCG 4 554 0

GCCGTCCGCG GCTGATGCGG ATCTTCGTCG ATCAGGTCCA GGTCCAGCTC GATAGGGCCG 45600

GAACCGCCCT GAGACGCCGC AGGAGCGTCC AGGAGGCTCG ACAGGTCGCC GATGCTATCC 4 5660

AACCCCAGGC CGGACGGCTG CGCCGCGCCT GCGGCTTCCT GAGCGGCCGC AGCGGTGTTT 45720

TTCTTGGTGG TCTTGGCTTG AGCCGCAGTC ATTGGGAAAT CTCCATCTTC GTGAACACGT 45780

AATCAGCCAG GGCGCGAACC TCTTTCGATG CCTTGCGCGC GGCCGTTTTC TTGATCTTCC 45840

AGACCGGCAC ACCGGATGCG AGGGCATCGG CGATGCTGCT GCGCAGGCCA ACGGTGGCCG 45900

GAATCATCAT CTTGGGGTAC GCGGCCAGCA GCTCGGCTTG GTGGCGCGCG TGGCGCGGAT 45960

TCCGCGCATC GACCTTGCTG GGCACCATGC CAAGGAATTG CAGCTTGGCG TTCTTCTGGC 4 6020

GCACGTTCGC AATGGTCGTG ACCATCTTCT TGATGCCCTG GATGCTGTAC GCCTCAAGCT 4 6080

CGATGGGGGA CAGCACATAG TCGGCCGCGA AGAGGGCGGC CGCCAGGCCG ACGCCAAGGG 4 6140

TCGGGGCCGT GTCGATCAGG CACACGTCGA AGCCTTGGTT CGCCAGGGCC TTGATGTTCG 4 6200

CCCCGAACAG CTCGCGGGCG TCGTCCAGCG ACAGCCGTTC GGCGTTCGCC AGTACCGGGT 4 6260

TGGACTCGAT GAGGGCGAGG CGCGCGGCCT GGCCGTCGCC GGCTGCGGGT GCGGTTTCGG 4 6320

TCCAGCCGCC GGCAGGGACA GCGCCGAACA GCTTGCTTGC ATGCAGGCCG GTAGCAAAGT 4 6380

CCTTGAGCGT GTAGGACGCA TTGCCCTGGG GGTCCAGGTC GATCACGGCA ACCCGCAAGC 4 6440

CGCGCTCGAA AAAGTCGAAG GCAAGATGCA CAAGGGTCGA AGTCTTGCCG ACGCCGCCTT 4 6500

TCTGGTTGGC CGTGACCAAA GTTTTCATCG TTTGGTTTCC TGTTTTTTCT TGGCGTCCGC 4 6560

TTCCCACTTC CGGACGATGT ACGCCTGATG TTCCGGCAGA ACCGCCGTTA CCCGCGCGTA 4 6620

CCCCTCGGGC AAGTTCTTGT CCTCGAACGC GGCCCACACG CGATGCACCG CTTGCGACAC 4 6680TGCGCCCCTG GTCAGTCCCA GCGACGTTGC GAACGTCGCC TGTGGCTTCC CATCGACTAA 4 6740

GACGCCCCGC GCTATCTCGA TGGTCTGCTG CCCCACTTCC AGCCCCTGGA TCGCCTCCTG 4 6800

GAACTGGCTT TCGGTAAGCC GTTTCTTCAT GGATAACACC CATAATTTGC TCCGCGCCTT 4 68 60

GGTTGAACAT AGCGGTGACA GCCGCCAGCA CATGAGAGAA GTTTAGCTAA ACATTTCTCG 4 6920CACGTCAACA CCTTTAGCCG CTAAAACTCG TCCTTGGCGT AACAAAACAA AAGCCCGGAA 4 6980

ACCGGGCTTT CGTCTCTTGC CGCTTATGGC TCTGCACCCG GCTCCATCAC CAACAGGTCG 47040

CGCACGCGCT TCACTCGGTT GCGGATCGAC ACTGCCAGCC CAACAAAGCC GGTTGCCGCC 4 7100

GCCGCCAGGA TCGCGCCGAT GATGCCGGCC ACACCGGCCA TCGCCCACCA GGTCGCCGCC 47160

TTCCGGTTCC ATTCCTGCTG GTACTGCTTC GCAATGCTGG ACCTCGGCTC ACCATAGGCT 4 7220

GACCGCTCGA TGGCGTATGC CGCTTCTCCC CTTGGCGTAA AACCCAGCGC CGCAGGCGGC 47280ATTGCCATGC TGCCCGCCGC TTTCCCGACC ACGACGCGCG CACCAGGCTT GCGGTCCAGA 4 7 340

CCTTCGGCCA CGGCGAGCTG CGCAAGGACA TAATCAGCCG CCGACTTGGC TCCACGCGCC 4 7 400TCGATCAGCT CTTGCACTCG CGCGAAATCC TTGGCCTCCA CGGCCGCCAT GAATCGCGCA 4 74 60

CGCGGCGAAG GCTCCGCAGG GCCGGCGTCG TGATCGCCGC CGAGAATGCC CTTCACCAAG 47520

TTCGACGACA CGAAAATCAT GCTGACGGCT ATCACCATCA TGCAGACGGA TCGCACGAAC 4 7 580CCGCTGAATT GAACACGAGC ACGGCACCCG CGACCACTAT GCCAAGAATG CCCAAGGTAA 4 7 640

AAATTGCCGG CCCCGCCATG AAGTCCGTGA ATGCCCCGAC GGCCGAAGTG AAGGGCAGGC 47700CGCCACCCAG GCCGCCGCCC TCACTGCCCG GCACCTGGTC GCTGAATGTC GATGCCAGCA 4 77 60

CCTGCGGCAC GTCAATGCTT CCGGGCGTCG CGCTCGGGCT GATCGCCCAT CCCGTTACTG 4 7820

CCCCGATCCC GGCAATGGCA AGGACTGCCA GCGCTGCCAT TTTTGGGGTG AGGCCGTTCG 4 7880

CGGCCGAGGG GCGCAGCCCC TGGGGGGATG GGAGGCCCGC GTTAGCGGGC CGGGAGGGTT 4 7 940

CGAGAAGGGG GGGCACCCCC CTTCGGCGTG CGCGGTCACG CGCACAGGGC GCAGCCCTGG 4 8000

TTAAAAACAA GGTTTATAAA TATTGGTTTA AAAGCAGGTT AAAAGACAGG TTAGCGGTGG 4 8060CCGAAAAACG GGCGGAAACC CTTGCAAATG CTGGATTTTC TGCCTGTGGA CAGCCCCTCA 48120

AATGTCAATA GGTGCGCCCC TCATCTGTCA GCACTCTGCC CCTCAAGTGT CAAGGATCGC 4 8180

GCCCCTCATC TGTCAGTAGT CGCGCCCCTC AAGTGTCAAT ACCGCAGGGC ACTTATCCCC 4 824 0

AGGCTTGTCC ACATCATCTG TGGGAAACTC GCGTAAAATC AGGCGTTTTC GCCGATTTGC 48300

GAGGCTGGCC AGCTCCACGT CGCCGGCCGA AATCGAGCCT GCCCCTCATC TGTCAACGCC 4 8360

GCGCCGGGTG AGTCGGCCCC TCAAGTGTCA ACGTCCGCCC CTCATCTGTC AGTGAGGGCC 4 8420

AAGTTTTCCG CGAGGTATCC ACAACGCCGG CGGCCGCGGT GTCTCGCACA CGGCTTCGAC 4 84 80

GGCGTTTCTG GCGCGTTTGC AGGGCCATAG ACGGCCGCCA GCCCAGCGGC GAGGGCAACC 4 8540

AGCCCGGTGA GCGTCGGAAA GGCGCTGGAA GCCCCGTAGC GACGCGGAGA GGGGCGAGAC 4 8 600AAGCCAAGGG CGCAGGCTCG ATGCGCAGCA CGACATAGCC GGTTCTCGCA AGGACGAGAA 4 8 660

TTTCCCTGCG GTGCCCCTCA AGTGTCAATG AAAGTTTCCA ACGCGAGCCA TTCGCGAGAG 48720

CCTTGAGTCC ACGCTAGATG AGAGCTTTGT TGTAGGTGGA CCAGTTGGTG ATTTTGAACT 4 8780

TTTGCTTTGC CACGGAACGG TCTGCGTTGT CGGGAAGATG CGTGATCTGA TCCTTCAACT 4 88 40

CAGCAAAAGT TCGATTTATT CAACAAAGCC ACGTTGTGTC TCAAAATCTC TGATGTTACA 4 8 900

TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA 4 8 960

ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAAC 4 8 997

Claims (34)

1. POLINUCLEOTÍDEO ISOLADO, caracterizado pelo fato deque compreende:(a) seqüência de nucleotídeos que codifica polipeptídeotransportador de nitrato com alta afinidade, em que o polipeptídeo contémseqüência de aminoácidos com pelo menos 80% de identidade de seqüênciascom base no método de alinhamento Clustal V em comparação com SEQ ID N0ou 49; ou(b) complemento da seqüência de nucleotídeos, em que ocomplemento e a seqüência de nucleotídeos contêm a mesma quantidade denucleotídeos e são 100% complementares.

2. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de aminoácidos do polipeptídeopossui pelo menos 85% de identidade de seqüências, com base no método dealinhamento Clustal V, em comparação com SEQ ID N0 36, 49 ou 92.

3. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de aminoácidos do polipeptídeopossui pelo menos 90% de identidade de seqüências, com base no método dealinhamento Clustal V, em comparação com SEQ ID N0 36, 49 ou 92.

4. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de aminoácidos do polipeptídeopossui pelo menos 95% de identidade de seqüências, com base no método dealinhamento Clustal V, em comparação com SEQ ID N0 36, 49 ou 92.

5. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de aminoácidos do polipeptídeopossui pelo menos 99% de identidade de seqüências, com base no método dealinhamento Clustal V, em comparação com SEQ ID N0 36, 49 ou 92.

6. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de aminoácidos do polipeptídeocompreende uma dentre SEQ ID N0 36, 49 ou 92.

7. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de nucleotídeos compreende umadentre SEQ ID N0 35 ou 48.

8. POLINUCLEOTÍDEO, de acordo com a reivindicação 1,caracterizado pelo fato de que a seqüência de nucleotídeos compreende pelomenos dois motivos selecionados a partir do grupo que consiste de SEQ ID N0 50, 51 e 52.

9. FRAGMENTO DE ÁCIDO NUCLÉICO ISOLADO,caracterizado pelo fato de que compreende promotor que consisteessencialmente de SEQ ID N0 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73,- 74, 89 ou 90 ou subfragmento substancialmente similar e funcionalmenteequivalente do mencionado promotor.

10. CONSTRUÇÃO DE DNA RECOMBINANTE, caracterizadapelo fato de que compreende polinucleotídeo isolado que codifica a varianteHAT conforme definida na reivindicação 1 ou seu subfragmento funcionalmenteequivalente, ligado operativamente a pelo menos uma seqüência reguladora.

11. CONSTRUÇÃO, de acordo com a reivindicação 10,caracterizada pelo fato de que a mencionada seqüência reguladoracompreende o promotor de acordo com a reivindicação 9.

12. PLANTA, caracterizada pelo fato de que compreende noseu genoma a construção de DNA recombinante conforme definida nareivindicação 10.

13. SEMENTE, caracterizada pelo fato de ser obtida a partir daplanta conforme definida na reivindicação 12.

14. PLANTA, de acordo com a reivindicação 12, caracterizadapelo fato de que a mencionada planta é selecionada a partir do grupo que consistede arroz, milho, sorgo, milho branco, centeio, soja, canola, trigo, cevada, aveia,feijão e nozes.

15. CÉLULA VEGETAL, caracterizada pelo fato de quecompreende no seu genoma a construção de DNA recombinante conformedefinida na reivindicação 10.

16. TECIDO VEGETAL, caracterizado pelo fato de quecompreende a célula vegetal conforme definida na reivindicação 15.

17. MÉTODO DE ISOLAMENTO DE FRAGMENTOS DEÁCIDO NUCLÉICO, que codificam polipeptídeos que alteram o transporte denitrato de planta, caracterizado pelo fato de que compreende:(a) comparação de SEQ ID N0 36, 49, 55 ou 58 com outrasseqüências de polipeptídeos que alteram o transporte de nitrato da planta;(b) identificação da(s) seqüência(s) conservada(s) de quatroou mais aminoácidos obtidos na etapa (a);(c) elaboração de oligômero(s) ou sonda(s) de nucleotídeosespecíficos de região com base nas seqüências conservadas identificadas naetapa (b); e(d) uso do(s) oligômero(s) ou sonda(s) de nucleotídeos daetapa (c) para isolar seqüências alterando o transporte de nitrato da planta pormeio de protocolos dependentes de seqüências.

18. MÉTODO DE MAPEAMENTO DE VARIAÇÕESGENÉTICAS, relacionadas à alteração do transporte de nitrato em plantas,caracterizado pelo fato de que compreende:(a) cruzamento de duas variedades de plantas; e(b) avaliação de variações genéticas com relação a:(i) seqüência de ácidos nucléicos selecionada a partirdo grupo que consiste de SEQ ID N0 35, 48, 54 ou 57; ou(ii) seqüência de ácidos nucléicos que codificampolipeptídeo que consiste de SEQ ID N0 36, 49, 55 ou 58;em plantas progenitoras resultantes do cruzamento da etapa (a),em que a avaliação é realizada utilizando método selecionado a partir do grupo queconsiste de: análise de RFLP, análise de SNP e análise com base em PCR.

19. MÉTODO DE CULTIVO MOLECULAR, caracterizado pelofato de ser para alterar o transporte de nitrato em plantas, que compreende:(a) cruzamento de duas variedades de plantas; e(b) avaliação de variações genéticas com relação a:(i) seqüência de ácidos nucléicos selecionada a partir10 do grupo que consiste de SEQ ID N0 35, 48, 54 ou 57; ou(ii) seqüência de ácidos nucléicos que codificapolipeptídeo selecionado a partir do grupo que consiste de SEQ ID N0 36, 49,-55 ou 58;em plantas progenitoras resultantes do cruzamento da etapa (a),em que a avaliação é realizada utilizando método selecionado a partir do grupo queconsiste de: análise de RFLP, análise de SNP e análise com base em PCR.

20. PLANTA DE MILHO, caracterizada pelo fato de quecompreende:(a) primeira construção de DNA recombinante quecompreende polinucleotídeo isolado que codifica polipeptídeo HAT, ligadooperativamente a pelo menos uma seqüência reguladora; e(b) pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado que codifica polipeptídeoNAR, ligado operativamente a pelo menos uma seqüência reguladora.

21. MÉTODO DE ALTERAÇÃO DO TRANSPORTE DENITROGÊNIO DAS PLANTAS, caracterizado pelo fato de que compreende:(a) transformação de planta com construção de DNArecombinante que compreende:(i) primeira construção de DNA recombinante quecompreende polinucleotídeo isolado que codifica polipeptídeo HAT, ligadooperativamente a pelo menos uma seqüência reguladora; e(ii) pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado que codifica polipeptídeoNAR, ligado operativamente a pelo menos uma seqüência reguladora;(b) cultivo da planta transformada de (a) sob condiçõesapropriadas para a expressão da construção de DNA recombinante; e(c) seleção das plantas transformadas que possuem transportede nitrato alterado.

22. VARIANTES DE HAT DE PLANTAS ALTERADAS,caracterizadas pelo fato de serem com propriedades cinéticas de absorção denitrato alteradas em comparação com HAT do tipo selvagem.

23. VARIANTES DE HAT, de acordo com a reivindicação 22,caracterizadas pelo fato de que as variantes possuem Km na faixa de 0,5 a 2mM de nitrato.

24. VARIANTES DE HAT, de acordo com a reivindicação 22,caracterizadas pelo fato de que as variantes possuem Vmax pelo menos duasa dez vezes mais alta em comparação com HAT do tipo selvagem.

25. VARIANTES DE HAT, de acordo com a reivindicação 22,caracterizadas pelo fato de que as variantes possuem Km na faixa de 0,5 a 2mM de nitrato e Vmax pelo menos duas a dez vezes mais alta em comparaçãocom HATs do tipo selvagem.

26. CONSTRUÇÃO DE DNA RECOMBINANTE, caracterizadapelo fato de que compreende polinucleotídeo isolado que codifica as variantesde HAT de acordo com uma das reivindicações 22 a 25, ligadasoperativamente a pelo menos uma seqüência reguladora.

27. CONSTRUÇÃO DE DNA RECOMBINANTE, caracterizadapelo fato de que compreende polinucleotídeo isolado que codifica as variantesde HAT de acordo com uma das reivindicações 22 a 25, ligadasoperativamente a pelo menos uma seqüência reguladora, em que amencionada seqüência reguladora compreende o promotor conforme definidona reivindicação 9.

28. PLANTA, caracterizada pelo fato de que compreende noseu genoma a construção de DNA recombinante conforme definida nareivindicação 26 ou 27.

29. SEMENTE, caracterizada pelo fato de ser obtida da planta,conforme definida na reivindicação 28.

30. PLANTA, de acordo com a reivindicação 28, caracterizadapelo fato de que a mencionada planta é selecionada a partir do grupo que consistede arroz, milho, sorgo, milho branco, centeio, soja, canola, trigo, cevada, aveia,feijão e nozes.

31. CÉLULA VEGETAL, caracterizada pelo fato de quecompreende no seu genoma a construção de DNA recombinante conformedefinida na reivindicação 26 ou 27.

32. TECIDO VEGETAL, caracterizado pelo fato de quecompreende a célula vegetal conforme definida na reivindicação 31.

33. PLANTA DE MILHO, caracterizada pelo fato de quecompreende:(a) primeira construção de DNA recombinante quecompreende a construção de DNA recombinante conforme definida nareivindicação 25 ou 26; e(b) pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado que codifica polipeptídeoNAR, ligado operativamente a pelo menos uma seqüência reguladora.

34. MÉTODO DE ALTERAÇÃO DO TRANSPORTE DENITROGÊNIO DAS PLANTAS, caracterizado pelo fato de que compreende:(a) transformação de planta com construção de DNArecombinante que compreende:(i) primeira construção de DNA recombinante quecompreende a construção de DNA recombinante conforme definida nareivindicação 26 ou 27; e(ii) pelo menos uma construção de DNA recombinanteadicional que compreende polinucleotídeo isolado que codifica polipeptídeoNAR1 ligado operativamente a pelo menos uma seqüência reguladora;(b) cultivo da planta transformada da etapa (a) sob condiçõesapropriadas para a expressão das construções de DNA recombinante; e(c) seleção das plantas transformadas que possuem transportede nitrato alterado.