BRPI0415646A - vetores retrovirais para o suprimento de rna de interferência - Google Patents

vetores retrovirais para o suprimento de rna de interferência

Info

Publication number
BRPI0415646A
BRPI0415646A BRPI0415646-3A BRPI0415646A BRPI0415646A BR PI0415646 A BRPI0415646 A BR PI0415646A BR PI0415646 A BRPI0415646 A BR PI0415646A BR PI0415646 A BRPI0415646 A BR PI0415646A
Authority
BR
Brazil
Prior art keywords
rna
retroviral vectors
supply
interference
vectors
Prior art date
Application number
BRPI0415646-3A
Other languages
English (en)
Inventor
Dorre Grueneberg
Gerard Bain
Nayantara Kothari
Original Assignee
Aventis Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Inc filed Critical Aventis Pharma Inc
Publication of BRPI0415646A publication Critical patent/BRPI0415646A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"VETORES RETROVIRAIS PARA O SUPRIMENTO DE RNA DE INTERFERêNCIA". O presente requerimento de patente de refere a vetores retrovirais e lentovirais, que compreendem um promotor para a polimerase RNA, um poli-ligante e um ácido nucléico que codifica para um RNA pequeno to tipo de grampo de cabelo (shRNA) específico para um gene alvo. Na ocasião da introdução do referido shRNA dentro de células, é induzida a degradação do RNA mensageiro do gene alvo através da interferência do RNA.
BRPI0415646-3A 2003-10-22 2004-10-21 vetores retrovirais para o suprimento de rna de interferência BRPI0415646A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51331303P 2003-10-22 2003-10-22
PCT/US2004/034932 WO2005042754A1 (en) 2003-10-22 2004-10-21 Retroviral vectors for delivery of interfering rna

Publications (1)

Publication Number Publication Date
BRPI0415646A true BRPI0415646A (pt) 2006-12-12

Family

ID=34549264

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0415646-3A BRPI0415646A (pt) 2003-10-22 2004-10-21 vetores retrovirais para o suprimento de rna de interferência

Country Status (13)

Country Link
US (2) US7612195B2 (pt)
EP (1) EP1678312A1 (pt)
JP (1) JP2007508842A (pt)
KR (1) KR20060107519A (pt)
CN (1) CN1871356B (pt)
AR (1) AR046598A1 (pt)
AU (2) AU2004286231B2 (pt)
BR (1) BRPI0415646A (pt)
CA (1) CA2542871C (pt)
IL (1) IL174804A0 (pt)
MX (1) MXPA06004428A (pt)
TW (2) TWI350311B (pt)
WO (1) WO2005042754A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2596715A1 (en) * 2005-02-01 2006-08-10 Medical Research Council Screening method
WO2007149246A2 (en) * 2006-06-12 2007-12-27 Massachusetts Institute Of Technology Cre-lox based gene knockdown constructs and methods of use thereof
US9043994B2 (en) 2007-03-13 2015-06-02 Massachusetts Institute Of Technology Cre-lox based gene knockdown constructs and methods of use thereof
WO2010022553A1 (en) * 2008-08-25 2010-03-04 Institute Of Basic Medical Sciences, Chinese Academy Of Medical Sciences An endogenous short hairpin rna and the use of the same
WO2013043442A1 (en) 2011-09-23 2013-03-28 Dharmacon, Inc. The introduction of modular vector elements during production of a lentivirus
CN103060374B (zh) * 2012-10-30 2014-05-07 中国农业科学院兰州兽医研究所 一种基于位点特异性重组的rna干扰载体及其构建方法和应用
CN105586360A (zh) * 2016-03-04 2016-05-18 武汉科技大学 一种同时表达小分子rna和蛋白质的双功能慢病毒表达载体及其制备方法和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624803A (en) * 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
US6624803B1 (en) * 1995-10-20 2003-09-23 Wisconsin Alumni Research Foundation Interface for electronic devices providing improved access for people with disabilities
CN1429911A (zh) * 2001-12-30 2003-07-16 李卫云 慢病毒载体系统，产生载体的方法及其应用
JP2005521393A (ja) * 2002-03-20 2005-07-21 マサチューセッツ インスティテュート オブ テクノロジー Ｈｉｖ治療
WO2004022722A2 (en) * 2002-09-06 2004-03-18 Massachusetts Institute Of Technology Lentiviral vectors, related reagents, and methods of use thereof
WO2004056966A2 (en) * 2002-12-18 2004-07-08 Salk Institute For Biological Studies Methods of inhibiting gene expression by rna interference

Also Published As

Publication number Publication date
EP1678312A1 (en) 2006-07-12
US7612195B2 (en) 2009-11-03
AR046598A1 (es) 2005-12-14
MXPA06004428A (es) 2006-07-03
US20060252153A1 (en) 2006-11-09
CA2542871A1 (en) 2005-05-12
AU2004286231A1 (en) 2005-05-12
KR20060107519A (ko) 2006-10-13
AU2004286231B2 (en) 2009-09-10
CA2542871C (en) 2010-11-16
WO2005042754A1 (en) 2005-05-12
US20100028996A1 (en) 2010-02-04
TW200523362A (en) 2005-07-16
JP2007508842A (ja) 2007-04-12
AU2009248456B2 (en) 2011-09-15
US7884200B2 (en) 2011-02-08
TW201111508A (en) 2011-04-01
AU2009248456A1 (en) 2010-01-07
CN1871356A (zh) 2006-11-29
TWI350311B (en) 2011-10-11
IL174804A0 (en) 2006-08-20
CN1871356B (zh) 2011-04-27

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Legal Events

Date Code Title Description
B25G Requested change of headquarter approved

Owner name: AVENTIS PHARMACEUTICALS, INC. (US)

Free format text: SEDE ALTERADA CONFORME SOLICITADO NA PETICAO NO 020070165509/RJ DE 23/11/2007.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]