BR9800827C1 - DNA encoding a protein, cell, and process to produce hexulose phosphate isomerase - Google Patents
DNA encoding a protein, cell, and process to produce hexulose phosphate isomeraseInfo
- Publication number
- BR9800827C1 BR9800827C1 BR9800827A BR9800827A BR9800827C1 BR 9800827 C1 BR9800827 C1 BR 9800827C1 BR 9800827 A BR9800827 A BR 9800827A BR 9800827 A BR9800827 A BR 9800827A BR 9800827 C1 BR9800827 C1 BR 9800827C1
- Authority
- BR
- Brazil
- Prior art keywords
- orf
- hexulose phosphate
- phosphate synthase
- cell
- gastri
- Prior art date
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
"DNA CODIFICANDO PARA UMA PROTEìNA, CéLULA, E, PROCESSO PARA PRODUZIR ISOMERASE DE FOSFATO DE HEXULOSE". A sintase de fosfato de hexulose foi purificado de <MU>Mycobacterium<MV> gastri_para determinar uma sequência de aminoácido da enzima. Os iniciadores de oligonucleotídeos a serem usados para PCR foram sintetizados em bases de informação da sequência de aminoácido obtida. Um fragmento de DNA, que foi amplificado por realização do PCR por uso de um gabarito de DNA genómico preparado a partir de <MU>Mycobacterium<MV> <MU>gastri<MV> foi usado como uma sonda de modo que a hibridização da colónia foi realizada com relação a uma coleção compreendendo fragmentos obtidos por digestão de DNA genómico de <MU>Mycobacterium<MV> <MU>gastri<MV> com <MU>Pst<MV>I para obter um clone positivo. Assim, o fragmento de DNA que contém ORF codificando sintase de fosfato de hexulose foi obtido. Além disso, o fragmento de DNA clonado continha outro ORF (ORF-2). O sistema de expressão de ORF-2 foi construído (pT-HPIS-1) e foi introduzido em célula de <MU>Escheichia coli<MV>. Após a indução da expressão de ORF-2, a atividade de sintase de fosfato de hexulose foi verificada no extrato de células abrigando pT-HPIS-1. Assim, o gene, que codificou para sintase de fosfato de hexulose, foi obtido junto com ORF que codificou para sintase de fosfato de hexulose."DNA CODING FOR A PROTEIN, CELL, AND PROCESS TO PRODUCE HEXULOSE PHOSPHATE ISOMERASE". The hexulose phosphate synthase was purified from <MU> Mycobacterium <MV> gastri_ to determine an amino acid sequence of the enzyme. The oligonucleotide primers to be used for PCR were synthesized in information bases of the obtained amino acid sequence. A DNA fragment, which was amplified by performing PCR using a genomic DNA template prepared from <MU> Mycobacterium <MV> <MU> gastri <MV> was used as a probe so that colony hybridization was performed with respect to a collection comprising fragments obtained by digesting genomic DNA from <MU> Mycobacterium <MV> <MU> gastri <MV> with <MU> Pst <MV> I to obtain a positive clone. Thus, the DNA fragment containing ORF encoding hexulose phosphate synthase was obtained. In addition, the cloned DNA fragment contained another ORF (ORF-2). The ORF-2 expression system was constructed (pT-HPIS-1) and was introduced into a <MU> Escheichia coli <MV> cell. After the induction of ORF-2 expression, hexulose phosphate synthase activity was verified in the cell extract harboring pT-HPIS-1. Thus, the gene, which encoded hexulose phosphate synthase, was obtained together with ORF which encoded hexulose phosphate synthase.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23313197 | 1997-08-28 | ||
| JP19480898A JP4122580B2 (en) | 1997-08-28 | 1998-07-09 | Hexulose phosphate isomerase gene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR9800827C1 true BR9800827C1 (en) | 2000-03-21 |
| BR9800827A BR9800827A (en) | 2001-06-26 |
Family
ID=26508745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9800827A BR9800827A (en) | 1997-08-28 | 1998-11-19 | DNA encoding for a protein, cell, and, proce. |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP4122580B2 (en) |
| BR (1) | BR9800827A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7078204B2 (en) * | 2000-06-21 | 2006-07-18 | Kyowa Hakko Kogyo Co., Ltd. | Glucose-6-phosphate dehydrogenase |
| JP2002017351A (en) * | 2000-07-07 | 2002-01-22 | Ajinomoto Co Inc | Phosphohexuloisomerase and its gene |
| JP2007068424A (en) * | 2005-09-05 | 2007-03-22 | Kyoto Univ | Gene encoding bifunctional formaldehyde-immobilizing enzyme |
| CN116334050B (en) * | 2019-11-20 | 2024-02-02 | 珠海联邦制药股份有限公司 | Artificially designed lysyl endonuclease, coding sequence and fermentation method |
-
1998
- 1998-07-09 JP JP19480898A patent/JP4122580B2/en not_active Expired - Fee Related
- 1998-11-19 BR BR9800827A patent/BR9800827A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BR9800827A (en) | 2001-06-26 |
| JPH11127869A (en) | 1999-05-18 |
| JP4122580B2 (en) | 2008-07-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application fees: dismissal - article 86 of industrial property law |
Free format text: REFERENTE A 8A E 9A ANUIDADES. |
|
| B08F | Application fees: dismissal - article 86 of industrial property law |
Free format text: ADDITIONAL INVENTOR S CERTIFICATE: REFERENTE 8O, 9O, 10O E 11O ANUIDADES. |
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| B08K | Maintenance of archiving: application remains archived as no request for restauration (art. 87) has been received. |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1905 DE 10/07/2007. |
|
| B08K | Maintenance of archiving: application remains archived as no request for restauration (art. 87) has been received. |
Free format text: ADDITIONAL INVENTOR S CERTIFICATE: REFERENTE AO DESPACHO 8.6 DA RPI 2016 DE 25/08/2009. |