AU6781700A - Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity - Google Patents

Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity

Info

Publication number
AU6781700A
AU6781700A AU67817/00A AU6781700A AU6781700A AU 6781700 A AU6781700 A AU 6781700A AU 67817/00 A AU67817/00 A AU 67817/00A AU 6781700 A AU6781700 A AU 6781700A AU 6781700 A AU6781700 A AU 6781700A
Authority
AU
Australia
Prior art keywords
bioseparations
generalizable
varying
self
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU67817/00A
Inventor
Georges Belfort
Marlene Belfort
Vicky Derbyshire
David Wood
Wei Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Health Research Institute
Original Assignee
Health Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Research Institute filed Critical Health Research Institute
Publication of AU6781700A publication Critical patent/AU6781700A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
AU67817/00A 1999-08-17 2000-08-17 Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity Abandoned AU6781700A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14925799P 1999-08-17 1999-08-17
US60149257 1999-08-17
PCT/US2000/022581 WO2001012820A1 (en) 1999-08-17 2000-08-17 Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity

Publications (1)

Publication Number Publication Date
AU6781700A true AU6781700A (en) 2001-03-13

Family

ID=22529449

Family Applications (1)

Application Number Title Priority Date Filing Date
AU67817/00A Abandoned AU6781700A (en) 1999-08-17 2000-08-17 Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity

Country Status (2)

Country Link
AU (1) AU6781700A (en)
WO (1) WO2001012820A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1613159A4 (en) 2003-03-27 2007-08-01 Ptc Therapeutics Inc Methods of identifying compounds that target trna splicing endonuclease and uses of said compounds as anti-fungal agents
EP2319318A3 (en) 2003-03-27 2011-08-17 PTC Therapeutics, Inc. Methods of identifying compounds that target TRNA splicing endonuclease and uses of said compounds as anti-proliferative agents
EP1649002A4 (en) 2003-07-02 2009-06-03 Ptc Therapeutics Inc Rna processing protein complexes and uses thereof
WO2007014162A2 (en) * 2005-07-21 2007-02-01 Abbott Laboratories Multiple gene expression including sorf constructs and methods with polyproteins, pro-proteins, and proteolysis
US9796967B2 (en) 2012-08-01 2017-10-24 Ohio State Innovation Foundation Compositions related to controllable intervening protein sequences (CIPS) comprising reversible zinc-binding motifs and inteins
EP2904101B1 (en) * 2012-10-03 2019-06-12 Agrivida, Inc. Intein-modified proteases, their production and industrial applications
RU2021134101A (en) 2014-04-03 2022-03-22 АйДжиЭм БАЙОСАЙЕНСИЗ, ИНК. MODIFIED J-CHAIN
EP3824903A1 (en) 2015-09-30 2021-05-26 IGM Biosciences Inc. Binding molecules with modified j-chain
DK3356401T3 (en) 2015-09-30 2020-09-07 Igm Biosciences Inc BINDING MOLECULES WITH MODIFIED J-CHAIN
WO2020125707A1 (en) * 2018-12-19 2020-06-25 Tsinghua University Mtu δi-cm intein variant and the use thereof
CN113528479B (en) * 2020-04-13 2023-01-24 中国科学院微生物研究所 Efficient preparation method of transglutaminase and special engineering bacteria thereof
CN115896147B (en) * 2022-10-11 2023-10-03 态创生物科技(广州)有限公司 Intein evolution systems and methods, corresponding mutant plasmids and reporter plasmids

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997001642A1 (en) * 1995-06-28 1997-01-16 New England Biolabs, Inc. Modified proteins and methods of their production
US5795731A (en) * 1996-08-26 1998-08-18 Health Research Incorporated Inteins as antimicrobial targets: genetic screens for intein function
US5981182A (en) * 1997-03-13 1999-11-09 Albert Einstein College Of Medicine Of Yeshiva University Vector constructs for the selection and identification of open reading frames
GB9708918D0 (en) * 1997-05-01 1997-06-25 Ppl Therapeutics Scotland Ltd Methods
AU4380599A (en) * 1998-06-26 2000-01-17 Ppl Therapeutics (Scotland) Limited Methods for production of recombinant polypeptides
EP1117693B1 (en) * 1998-09-30 2006-05-17 New England Biolabs, Inc. Intein mediated peptide ligation
EP1141250B1 (en) * 1998-12-18 2006-03-01 The Penn State Research Foundation Intein-mediated cyclization of peptides
DE60040524D1 (en) * 1999-02-12 2008-11-27 New England Biolabs Inc INTEIN-MEDIATED LIGATION OF EXPRESSED PROTEINS

Also Published As

Publication number Publication date
WO2001012820A1 (en) 2001-02-22

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase