ATE373082T1 - PRE-ADIPOSE CELL LINES - Google Patents

PRE-ADIPOSE CELL LINES

Info

Publication number
ATE373082T1
ATE373082T1 AT01955350T AT01955350T ATE373082T1 AT E373082 T1 ATE373082 T1 AT E373082T1 AT 01955350 T AT01955350 T AT 01955350T AT 01955350 T AT01955350 T AT 01955350T AT E373082 T1 ATE373082 T1 AT E373082T1
Authority
AT
Austria
Prior art keywords
immortalized
cell lines
cells
adipose
methods
Prior art date
Application number
AT01955350T
Other languages
German (de)
Inventor
Christian Darimont
Katherine Mace
Andrea Pfeifer
Original Assignee
Nestle Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestle Sa filed Critical Nestle Sa
Application granted granted Critical
Publication of ATE373082T1 publication Critical patent/ATE373082T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to new immortalized human pre-adipose cell lines capable of differentiating into adipose cells and methods of obtaing the immortalized cells. In particular, the present invention pertains to immortalized pre-adipocyte cell lines derived from white adipose tissue and methods of producing the cell lines. The immortalized pre-adipocyte cells are capable of maturing into immortalized white adipose cells useful in developing drugs, food ingredients and supplements against obesity, diabetes and cardiovascular diseases.
AT01955350T 2000-07-18 2001-07-13 PRE-ADIPOSE CELL LINES ATE373082T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP00115489 2000-07-18

Publications (1)

Publication Number Publication Date
ATE373082T1 true ATE373082T1 (en) 2007-09-15

Family

ID=8169292

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01955350T ATE373082T1 (en) 2000-07-18 2001-07-13 PRE-ADIPOSE CELL LINES

Country Status (8)

Country Link
US (1) US7294509B2 (en)
EP (1) EP1303589B1 (en)
JP (1) JP2004504024A (en)
CN (1) CN100595274C (en)
AT (1) ATE373082T1 (en)
AU (1) AU2001277539A1 (en)
DE (1) DE60130464T2 (en)
WO (1) WO2002006450A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050136039A1 (en) * 2002-03-22 2005-06-23 Joslin Diabetes Center, Inc. Adipocytes and uses thereof
WO2003082006A2 (en) * 2002-03-22 2003-10-09 Joslin Diabetes Center, Inc. Methods and compositions for preventing obesity and obesity related disorders
ES2559430T3 (en) 2002-06-18 2016-02-12 Eisai R&D Management Co., Ltd. Primary adipocytes cultured for gene therapy
WO2008103807A2 (en) * 2007-02-21 2008-08-28 Cedars-Sinai Medical Center Methods of producing preadipocytes and increasing the proliferation of adult adipose stem/progenitor cells
CN101259266B (en) * 2008-04-14 2010-04-14 山东大学 Preadipocyte heterologous vaccine and preparation method and application thereof
US8883210B1 (en) 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
CN102140437A (en) * 2011-01-07 2011-08-03 西北农林科技大学 Culturing method for quickly dedifferentiating porcine mature fat cell into precursor fat cell
US8834928B1 (en) 2011-05-16 2014-09-16 Musculoskeletal Transplant Foundation Tissue-derived tissugenic implants, and methods of fabricating and using same
KR101422559B1 (en) * 2012-03-09 2014-07-24 창원대학교 산학협력단 Culture medium of adipose-derived stem cell, method for preparing the same, and composition for promoting hair growth comprising the same
CN102876717B (en) * 2012-10-29 2014-06-11 东北农业大学 Method for building immortalized preadipocytes of chicken
AU2014253920B2 (en) 2013-04-19 2019-07-04 Biorestorative Therapies, Inc. Human brown adipose derived stem cells and uses
WO2015017500A1 (en) 2013-07-30 2015-02-05 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
ES2700147T3 (en) * 2014-12-04 2019-02-14 Intervet Int Bv Immortalized chicken embryo fibroblasts
CA2986702C (en) 2015-05-21 2023-04-04 David Wang Modified demineralized cortical bone fibers
US10912864B2 (en) 2015-07-24 2021-02-09 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
US11052175B2 (en) 2015-08-19 2021-07-06 Musculoskeletal Transplant Foundation Cartilage-derived implants and methods of making and using same
US11518982B2 (en) 2015-12-01 2022-12-06 Adiposeeds, Inc. Method for manufacturing mesenchymal cell line derived from vertebrate animal adipose tissue
WO2017097983A1 (en) * 2015-12-11 2017-06-15 Intervet International B.V. Immortalised chicken embryonic epithelial kidney cells
EP3739323A4 (en) 2018-01-24 2021-03-17 Sniper (Beijing) Medical Technologies Co., Ltd Microdroplet container, method for preparing microdroplet container, microdroplet spreading method, microdroplet formation kit, temperature control device, oil phase composition for microdroplet formation and treatment method therefor
WO2019144894A1 (en) 2018-01-24 2019-08-01 北京光阱管理咨询合伙企业（有限合伙） Motion control mechanism, liquid spitting pipette tip, micro-droplet generating device, fluid driving mechanism and fluid driving method, micro-droplet generating method, and surface treatment method for liquid spitting pipette tip
CN110064443B (en) * 2018-01-24 2021-07-30 思纳福(北京)医疗科技有限公司 Micro-droplet tiling method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830682A (en) * 1994-09-09 1998-11-03 Zymogenetics Preparation of immortalized cells
FR2733513B1 (en) * 1995-04-25 1997-07-18 Centre Nat Rech Scient IMMORTALIZED LINES OF CELLS FROM HUMAN FATTY TISSUE, THEIR PREPARATION PROCESS AND THEIR APPLICATIONS
US5827740A (en) * 1996-07-30 1998-10-27 Osiris Therapeutics, Inc. Adipogenic differentiation of human mesenchymal stem cells
JP5055611B2 (en) * 1998-09-09 2012-10-24 学校法人日本大学 Preadipocyte cell line
WO2000024866A2 (en) * 1998-10-26 2000-05-04 Institut National De La Sante Et De La Recherche Medicale (Inserm) Multipotential human medullary stromal cell line of and uses thereof

Also Published As

Publication number Publication date
EP1303589A1 (en) 2003-04-23
US7294509B2 (en) 2007-11-13
US20030175957A1 (en) 2003-09-18
DE60130464T2 (en) 2008-06-12
EP1303589B1 (en) 2007-09-12
WO2002006450A1 (en) 2002-01-24
CN1458973A (en) 2003-11-26
AU2001277539A1 (en) 2002-01-30
JP2004504024A (en) 2004-02-12
CN100595274C (en) 2010-03-24
DE60130464D1 (en) 2007-10-25

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