ATE323768T1 - FAT ACYL-COA: FAT ACYL CARBON O-ACYL TRANSFERASE - Google Patents

FAT ACYL-COA: FAT ACYL CARBON O-ACYL TRANSFERASE

Info

Publication number
ATE323768T1
ATE323768T1 AT98925259T AT98925259T ATE323768T1 AT E323768 T1 ATE323768 T1 AT E323768T1 AT 98925259 T AT98925259 T AT 98925259T AT 98925259 T AT98925259 T AT 98925259T AT E323768 T1 ATE323768 T1 AT E323768T1
Authority
AT
Austria
Prior art keywords
acyl
coa
wax
fat
nucleic acid
Prior art date
Application number
AT98925259T
Other languages
German (de)
Inventor
Kathryn Dennis Lardizabal
James George Metz
Michael W Lassner
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Application granted granted Critical
Publication of ATE323768T1 publication Critical patent/ATE323768T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fats And Perfumes (AREA)

Abstract

By this invention, nucleic acid sequences encoding for fatty acyl-CoA: fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest is are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.
AT98925259T 1997-06-05 1998-06-05 FAT ACYL-COA: FAT ACYL CARBON O-ACYL TRANSFERASE ATE323768T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4865197P 1997-06-05 1997-06-05

Publications (1)

Publication Number Publication Date
ATE323768T1 true ATE323768T1 (en) 2006-05-15

Family

ID=21955701

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98925259T ATE323768T1 (en) 1997-06-05 1998-06-05 FAT ACYL-COA: FAT ACYL CARBON O-ACYL TRANSFERASE

Country Status (9)

Country Link
US (1) US6596538B1 (en)
EP (1) EP0986647B1 (en)
JP (1) JP4126102B2 (en)
KR (1) KR20010013426A (en)
CN (1) CN1255541C (en)
AT (1) ATE323768T1 (en)
CA (1) CA2292768C (en)
DE (1) DE69834246T2 (en)
WO (1) WO1998055632A1 (en)

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US6483008B1 (en) * 1990-08-15 2002-11-19 Calgene Llc Methods for producing plants with elevated oleic acid content
US7332311B2 (en) 1994-06-23 2008-02-19 Calgene Llc Fatty acyl-CoA: fatty alcohol acyltransferases
US6492509B1 (en) 1994-06-23 2002-12-10 Calgene Llc Fatty acyl-CoA: fatty alcohol acyltransferases
JP4126102B2 (en) 1997-06-05 2008-07-30 カルジーン エル エル シー Fatty acyl-CoA: fatty alcohol acyltransferase
AU2166400A (en) * 1998-12-04 2000-06-19 Calgene Llc Diacylglycerol acyl transferase proteins
EP1173583A1 (en) * 1999-05-04 2002-01-23 Cargill, Incorporated Plant acyltransferases
US6995301B1 (en) 1999-05-04 2006-02-07 Cargill, Incorporated Plant acyltransferases
MXPA04008788A (en) * 2002-03-25 2005-06-20 Teijin Ltd Method of assaying coenzymes a in biological sample.
WO2004087902A2 (en) 2003-03-31 2004-10-14 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
EP1718663B1 (en) 2004-01-20 2011-07-13 Monsanto Technology, LLC Chimeric promoters for use in plants
EP2371967B1 (en) 2005-03-18 2015-06-03 DSM IP Assets B.V. Production of carotenoids in oleaginous yeast and fungi
AU2007253903B2 (en) 2006-05-16 2012-07-19 Monsanto Technology Llc Use of non-agrobacterium bacterial species for plant transformation
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
EP2078092A2 (en) 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008112633A2 (en) 2007-03-09 2008-09-18 Monsanto Technology Llc Method of meristem excision and transformation
MX2010011003A (en) 2008-04-07 2010-11-01 Monsanto Technology Llc Plant regulatory elements and uses thereof.
JP2011522525A (en) 2008-05-16 2011-08-04 エルエス９・インコーポレイテッド Methods and compositions for producing hydrocarbons
WO2010099431A2 (en) 2009-02-27 2010-09-02 Monsanto Technology Llc Hydroponic apparatus and methods of use
CA2759273C (en) 2009-04-27 2018-01-09 Ls9, Inc. Production of fatty acid esters
EP2821492A3 (en) 2009-05-13 2015-04-08 BASF Plant Science Company GmbH Acyltransferases and uses thereof in fatty acid production
WO2011050271A1 (en) 2009-10-23 2011-04-28 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
CA3209765A1 (en) 2010-01-14 2011-07-21 Monsanto Technology Llc Plant regulatory elements and uses thereof
EP2585603B1 (en) 2010-06-25 2017-12-20 BASF Plant Science Company GmbH Acyltransferases and uses therof in fatty acid production
ES2657233T3 (en) 2010-08-30 2018-03-02 Dow Agrosciences, Llc Sugarcane bacilliform viral enhancer (SCBV) and its use in functional plant genomics
US10407685B2 (en) 2010-12-17 2019-09-10 Monsanto Technology Llc Methods for improving competency of plant cells
HUE035893T2 (en) 2011-03-25 2018-05-28 Monsanto Technology Llc Plant regulatory elements and uses thereof
EP4186977A1 (en) 2011-05-13 2023-05-31 Monsanto Technology LLC Plant regulatory elements and uses thereof
KR101399945B1 (en) * 2012-02-20 2014-05-29 동아대학교 산학협력단 WES gene from soil metagenome and uses thereof
AR090204A1 (en) 2012-02-29 2014-10-29 Dow Agrosciences Llc BACILIFORM VIRAL BOX OF SUGAR CANE (SCBV) AND ITS USE IN THE FUNCTIONAL GENOMICS OF PLANTS
US9663793B2 (en) 2012-04-20 2017-05-30 Monsanto Technology, Llc Plant regulatory elements and uses thereof
MX352668B (en) 2012-12-19 2017-12-01 Monsanto Technology Llc Plant regulatory elements and uses thereof.
ES2769898T3 (en) 2013-03-14 2020-06-29 Monsanto Technology Llc Regulatory elements in plants and their uses
CN115992136A (en) 2013-03-14 2023-04-21 孟山都技术公司 Plant regulatory element and use thereof
BR112016001026A2 (en) * 2013-07-19 2017-10-24 Cargill Inc genetically modified organism
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN115094081A (en) 2014-11-14 2022-09-23 巴斯夫植物科学有限公司 Materials and methods for increasing tocopherol content in seed oil
EP3274462A4 (en) 2015-03-26 2018-12-26 The Texas A&M University System Conversion of lignin into bioplastics and lipid fuels
CA2992488A1 (en) 2015-08-28 2017-03-09 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
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JP7123803B2 (en) 2016-03-11 2022-08-23 モンサント テクノロジー エルエルシー PLANT REGULATORY ELEMENTS AND USES THEREOF
JP7078551B2 (en) 2016-05-24 2022-05-31 モンサント テクノロジー エルエルシー Plant regulatory elements and their use
US20190359992A1 (en) 2016-06-28 2019-11-28 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
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CN110494566A (en) 2017-02-02 2019-11-22 嘉吉公司 Generate the genetically modified cell of C6-C10 derivative of fatty acid
US20200332311A1 (en) 2018-01-12 2020-10-22 The Texas A&M University System Increasing plant bioproduct yield
WO2022204466A1 (en) 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a prokaryotic system
AR125216A1 (en) 2021-03-26 2023-06-28 Flagship Pioneering Innovations Vii Llc PRODUCTION OF CIRCULAR POLYRIBONUCLEOTIDES IN A EUKARYOTA SYSTEM
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JP4126102B2 (en) 1997-06-05 2008-07-30 カルジーン エル エル シー Fatty acyl-CoA: fatty alcohol acyltransferase

Also Published As

Publication number Publication date
US6596538B1 (en) 2003-07-22
WO1998055632A1 (en) 1998-12-10
CN1301304A (en) 2001-06-27
CN1255541C (en) 2006-05-10
EP0986647A1 (en) 2000-03-22
DE69834246D1 (en) 2006-05-24
JP2002513293A (en) 2002-05-08
CA2292768C (en) 2011-11-29
CA2292768A1 (en) 1998-12-10
JP4126102B2 (en) 2008-07-30
EP0986647B1 (en) 2006-04-19
KR20010013426A (en) 2001-02-26
DE69834246T2 (en) 2007-02-01

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