ATE175975T1 - DISPLACEMENT CHROMATOGRAPHIC METHOD AND PURIFIED HEMOGLOBIN - Google Patents

DISPLACEMENT CHROMATOGRAPHIC METHOD AND PURIFIED HEMOGLOBIN

Info

Publication number
ATE175975T1
ATE175975T1 AT94926756T AT94926756T ATE175975T1 AT E175975 T1 ATE175975 T1 AT E175975T1 AT 94926756 T AT94926756 T AT 94926756T AT 94926756 T AT94926756 T AT 94926756T AT E175975 T1 ATE175975 T1 AT E175975T1
Authority
AT
Austria
Prior art keywords
hemoglobin
column
admixtures
stage
chromatographic method
Prior art date
Application number
AT94926756T
Other languages
German (de)
Inventor
Diana Pliura
Diane Wiffen
Salman Ashraf
Anthony A Magnin
Original Assignee
Hemosol Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002106612A external-priority patent/CA2106612C/en
Application filed by Hemosol Inc filed Critical Hemosol Inc
Application granted granted Critical
Publication of ATE175975T1 publication Critical patent/ATE175975T1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to the chromatographic purification of hemoglobin. The method for purifying hemoglobin from the raw solution containing the admixture of proteinaceous origin provides for the use of ion-exchange chromatography. The raw solution containing 0.1-20% hemoglobin. At the first stage, anion-exchanging column is used when the admixtures are more acidic than hemoglobin, or cation-exchanging column is used when the admixtures are more alkaline than hemoglobin. The continuous flow of the solution results first in the complete saturation of the column with hemoglobin and the admixtures with high affinity to the column, then the column is overloaded following the substitution of hemoglobin. At the second stage, the ion-exchanger alternative to that used at the first stage is employed. Again, upon the complete saturation and overloading of the column, hemoglobin is substituted.
AT94926756T 1993-09-21 1994-09-20 DISPLACEMENT CHROMATOGRAPHIC METHOD AND PURIFIED HEMOGLOBIN ATE175975T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002106612A CA2106612C (en) 1993-09-21 1993-09-21 Displacement chromatography process

Publications (1)

Publication Number Publication Date
ATE175975T1 true ATE175975T1 (en) 1999-02-15

Family

ID=4152343

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94926756T ATE175975T1 (en) 1993-09-21 1994-09-20 DISPLACEMENT CHROMATOGRAPHIC METHOD AND PURIFIED HEMOGLOBIN

Country Status (9)

Country Link
JP (1) JP4057049B2 (en)
KR (1) KR100361894B1 (en)
AT (1) ATE175975T1 (en)
DE (1) DE69416103T2 (en)
DK (1) DK0668877T3 (en)
ES (1) ES2126775T3 (en)
HU (1) HU218159B (en)
RU (1) RU2145873C1 (en)
UA (1) UA35599C2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8153009B2 (en) * 2005-04-22 2012-04-10 Waters Technologies Corporation Apparatus and methods for mass-spectrometric directed purification of biopolymers
LT2462158T (en) 2009-08-06 2018-03-26 F. Hoffmann-La Roche Ag Method to improve virus removal in protein purification
US20130331554A1 (en) * 2010-11-15 2013-12-12 Biogen Idec Inc. Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography
TR201815709T4 (en) 2011-12-22 2018-11-21 Hoffmann La Roche Ion exchange membrane chromatography.
TWI675041B (en) * 2013-05-06 2019-10-21 法商賽諾菲公司 Continuous multistep process for purifying antibodies
TW202128264A (en) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 Cation chromatography using predicted elution buffer salt concentration
CN114712894B (en) * 2022-04-08 2024-04-30 无锡博慧斯生物医药科技有限公司 Activating solution, activating method and application of glycosylated hemoglobin chromatographic column

Also Published As

Publication number Publication date
UA35599C2 (en) 2001-04-16
JP4057049B2 (en) 2008-03-05
DE69416103T2 (en) 1999-08-05
JPH08503714A (en) 1996-04-23
HU9501479D0 (en) 1995-07-28
HU218159B (en) 2000-06-28
DK0668877T3 (en) 1999-09-13
RU2145873C1 (en) 2000-02-27
DE69416103D1 (en) 1999-03-04
ES2126775T3 (en) 1999-04-01
RU95112503A (en) 1996-12-20
HUT72924A (en) 1996-06-28
KR100361894B1 (en) 2003-05-12

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Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee