AR003122A1 - A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS. - Google Patents

A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS.

Info

Publication number
AR003122A1
AR003122A1 ARP960102507A AR10250796A AR003122A1 AR 003122 A1 AR003122 A1 AR 003122A1 AR P960102507 A ARP960102507 A AR P960102507A AR 10250796 A AR10250796 A AR 10250796A AR 003122 A1 AR003122 A1 AR 003122A1
Authority
AR
Argentina
Prior art keywords
purification
isolation
fermentators
plasmids
isolated
Prior art date
Application number
ARP960102507A
Other languages
Spanish (es)
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of AR003122A1 publication Critical patent/AR003122A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describe un proceso para la aislación y purificación a gran escala de ADN de plasmidios a partir de fermentaciones microbianas a gran escala. Todaslas tres formas de ADN de plasmidio: super-enrollado (forma 1), círculo abierto o relajado (forma2) y linearizado (forma 3) son aislables individualmenteusando el proceso revelado. Se provee, mediante el proceso revelado, ADN altamente purificado, adecuado para incluirlo en composiciones farmacéuticas.A process for the large-scale isolation and purification of plasmid DNA from large-scale microbial fermentations is described. All three forms of plasmid DNA: super-coiled (form 1), open or relaxed circle (form 2), and linearized (form 3) are individually isolatable using the disclosed process. Highly purified DNA suitable for inclusion in pharmaceutical compositions is provided by the disclosed process.

ARP960102507A 1995-05-19 1996-05-09 A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS. AR003122A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19

Publications (1)

Publication Number Publication Date
AR003122A1 true AR003122A1 (en) 1998-07-08

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP960102507A AR003122A1 (en) 1995-05-19 1996-05-09 A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS.

Country Status (18)

Country Link
EP (1) EP0827536A1 (en)
JP (1) JPH11505707A (en)
KR (1) KR19990014924A (en)
CN (1) CN1190435A (en)
AR (1) AR003122A1 (en)
AU (1) AU709003B2 (en)
CA (1) CA2220867A1 (en)
CZ (1) CZ366197A3 (en)
EA (1) EA000785B1 (en)
HR (1) HRP960222A2 (en)
HU (1) HUP9802557A3 (en)
MX (1) MX9708967A (en)
NO (1) NO975280L (en)
NZ (1) NZ309231A (en)
PL (1) PL323475A1 (en)
SK (1) SK155797A3 (en)
WO (1) WO1996036706A1 (en)
YU (1) YU29796A (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2192342A1 (en) * 1994-07-15 1996-02-01 Ann L. Lee A method for large scale plasmid purification
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (en) * 1996-09-11 1998-12-04 Transgene Sa PROCESS FOR THE PREPARATION OF PLASMID DNA
SE9703532D0 (en) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
AU4314099A (en) * 1998-06-01 1999-12-20 Immune Response Corporation, The Novel method of large scale plasmid purification
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
ATE309344T1 (en) 2001-01-19 2005-11-15 Mueller Drm Ag METHOD FOR PREPARING BIOMASS FOR PRODUCING CELL LYSATE CONTAINING PLASMID DNA
GB0107634D0 (en) 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
CA2595594C (en) 2005-01-31 2012-05-01 Merck & Co., Inc. Upstream and a downstream purification process for large scale production of plasmid dna
JP2006238822A (en) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd Method for separating and purifying nucleic acid
DE102005059315A1 (en) * 2005-12-09 2007-06-14 Qiagen Gmbh Process for the enrichment of short-chain nucleic acids
CN100439497C (en) * 2006-05-26 2008-12-03 吉林大学 Purification method adapted to mass production of plasmid DNA
EP2081442B1 (en) * 2006-10-10 2016-08-10 TrovaGene, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
CN101702938A (en) * 2007-05-23 2010-05-05 Vgx药品公司 Composition comprising high concentration of biologically active molecules and processes for preparing the same
CN101070543B (en) * 2007-06-01 2010-12-29 广东药学院 Super spirial plasmid separation purifying process
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
EP2556143B1 (en) * 2010-04-08 2016-12-07 Qiagen GmbH Method for isolating and purifying nucleic acids
JP2013528786A (en) 2010-04-08 2013-07-11 キアゲン ゲーエムベーハー Chromatographic device and method for isolating and purifying nucleic acids
JP5624487B2 (en) * 2011-01-31 2014-11-12 横河電機株式会社 Nucleic acid extraction method
ES2656947T3 (en) 2011-04-27 2018-03-01 Merck Patent Gmbh Method to lyse cells
CN104099321A (en) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 Preparation method of lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD
CN106884011B (en) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 Combined liquid chromatography separation method for large-scale plasmid purification
CN111721871A (en) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 High-resolution detection method for plasmid supercoiled DNA content
CN112798697B (en) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 Detection method for supercoiled structure purity of lentivirus packaging system helper plasmid
CN114456940B (en) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 Method for improving plasmid stability in escherichia coli cracking process

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0800830A3 (en) * 1989-11-03 1999-03-17 Vanderbilt University Method of in vivo delivery of functioning foreign genes
EP0646178A1 (en) * 1992-06-04 1995-04-05 The Regents Of The University Of California expression cassette with regularoty regions functional in the mammmlian host
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
CA2192342A1 (en) * 1994-07-15 1996-02-01 Ann L. Lee A method for large scale plasmid purification

Also Published As

Publication number Publication date
JPH11505707A (en) 1999-05-25
WO1996036706A1 (en) 1996-11-21
HUP9802557A3 (en) 2000-04-28
NO975280L (en) 1998-01-16
CA2220867A1 (en) 1996-11-21
YU29796A (en) 1999-06-15
PL323475A1 (en) 1998-03-30
KR19990014924A (en) 1999-02-25
CN1190435A (en) 1998-08-12
HRP960222A2 (en) 1997-12-31
EA199700399A1 (en) 1998-06-25
HUP9802557A2 (en) 1999-02-01
MX9708967A (en) 1998-03-31
NZ309231A (en) 1999-10-28
NO975280D0 (en) 1997-11-18
SK155797A3 (en) 1998-07-08
EA000785B1 (en) 2000-04-24
CZ366197A3 (en) 1998-04-15
EP0827536A1 (en) 1998-03-11
AU709003B2 (en) 1999-08-19
AU5921996A (en) 1996-11-29

Similar Documents

Publication Publication Date Title
AR003122A1 (en) A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS.
DK0923592T3 (en) Methods for purifying nucleic acids
ATE277175T1 (en) A PROCESS FOR LARGE SCALE PLASMIDS CLEANING
ATE99358T1 (en) ALPHA-1 ANTI-TRYPSIN MUTANTS AND DNA ENCODING THEREFORE AND PRODUCTION SAME, AND THERAPEUTIC COMPOSITIONS USING SUCH MUTANTS AND THEIR PRODUCTION.
NO943038D0 (en) Method of Preparation of Virus Resistant Plants via Introduction of Untranslatable Plus RNA
AU2002354853A1 (en) Process for the preparation of l-amino acids using strains of the enterobacteriaceae family
PT937098E (en) IN VITRO RECOMBINATION CLONING USING MODIFIED RECOMBINATION PLACES
ATE131161T1 (en) SUBSTANCE PF 1022, METHOD FOR THE PRODUCTION THEREOF AND ANTHELMINTIC COMPOSITION CONTAINING THIS SUBSTANCE.
DK1042340T3 (en) 6-O-B-alkylerythromycinoxim
DK0653436T3 (en) 5-Methyluridine Large Scale Preparation of 2 ', 3'-Didehydro-3'-Deoxythymidine (d4T)
AU6766487A (en) Purification of pertussis antigens
FR2641285B1 (en) GLUCOSE-6-PHOSPHATE DEHYDROGENASE GENE, PLASMID AND MICROORGANISM CONTAINING THE SAME AND PROCESS FOR THE PREPARATION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE
FR2768736B1 (en) NOVEL TRICYCLIC COMPOUNDS, THEIR PREPARATION PROCESS AND INTERMEDIATES THEREOF, THEIR APPLICATION AS MEDICAMENTS AND THE PHARMACEUTICAL COMPOSITIONS CONTAINING THEM
CA2226457A1 (en) Oligonucleotides specific for human papilloma virus
FR2747680B1 (en) NOVEL STEROIDS, THEIR APPLICATION AS MEDICAMENTS, THEIR PREPARATION PROCESS, THE INTERMEDIATES THEREOF AND THE PHARMACEUTICAL COMPOSITIONS CONTAINING THEM
DE69331216D1 (en) PLANT RESISTANT TO AT LEAST TWO VIRUSES AND THEIR PREPARATION
NO20004613L (en) Method of Preparation of a Tetrapeptide
WO1994020604A3 (en) Agricultural-chemical-producing endosymbiotic microorganisms and method of preparing and using same
HUP0105166A2 (en) Substances wk-5344a and wk-5344b and process for producing the same
ES8604022A1 (en) Process for the isolation and purification of alpha-interferons.
JPS5459392A (en) Novel nuclease and its preparation
ATE346920T1 (en) NEW IMMUNO-REGULATORY PROTEIN LST-1
JPS5539730A (en) Preparation of ubiquinone q10
FR2681598B1 (en) NOVEL DECAHYDROQUINOLEIN DERIVATIVES, THEIR PREPARATION PROCESS, THE PREPARATION INTERMEDIATES, THEIR APPLICATION AS MEDICAMENTS AND THE COMPOSITIONS CONTAINING THEM.
DE3882654D1 (en) ENZYME INHIBITORS AND METHOD FOR THE PRODUCTION THEREOF.