Tools (written in C using htslib) for manipulating next-generation sequencing data

Toolkit for processing sequences in FASTA/Q formats

Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).

Simple oligo analysis and primer design

Constructing a pangenome gene graph

sensitive and precise assembly of short sequencing reads

PAired-eND Assembler for DNA sequences

Flash2 has some improvements from flash_1 including new logic from innie and outie overlaps as well as some initial steps for flash for amplicons

Merging paired-end reads and removing adapters