This repository provides training and testing scripts for the paper DEEMD: Drug Efficacy Estimation against SARS-CoV-2 based on cell Morphology with Deep multiple instance learning.

Drug repurposing can accelerate the identification of effective compounds for clinical use against SARS-CoV-2, with the advantage of pre-existing clinical safety data and an established supply chain. RNA viruses such as SARS-CoV-2 manipulate cellular pathways and induce reorganization of subcellular structures to support their life cycle. These morphological changes can be quantified using bioimaging techniques. In this work, we developed DEEMD: a computational pipeline using deep neural network models within a multiple instance learning (MIL) framework, to identify putative treatments effective against SARS-CoV-2 based on morphological analysis of the publicly available RxRx19a dataset. This dataset consists of fluorescence microscopy images of SARS-CoV-2 non-infected cells and infected cells, with and without drug treatment. DEEMD first extracts discriminative morphological features to generate cell morphological profiles from the non-infected and infected cells. These morphological profiles are then used in a statistical model to estimate the applied treatment efficacy on infected cells based on similarities to non-infected cells. DEEMD is capable of localizing infected cells via weak supervision without any expensive pixel-level annotations. DEEMD identifies known SARS-CoV-2 inhibitors, such as Remdesivir and Aloxistatin, supporting the validity of our approach. DEEMD is scalable to process and screen thousands of treatments in parallel and can be explored for other emerging viruses and datasets to rapidly identify candidate antiviral treatments in the future.

Get the images and the meta data from Recursion website. Include a column named path into the meta dataframe which contains the relative path to the images files. Use this csv file along with parameter base_path to properly load the dataset into the dataloader.

To transfer DEEMD pipeline to another dataset follow the guideline below. This guide assumes that you are working with a dataset of fluorescence microscopy images for drug repurposing, however, the deep multiple instance learning framework can be applied to other problems as well.

1. Create a csv file that includes meta data of the samples in the dataset. In particular, this should include a path column for loading the samples.
2. Split the csv file into train, validation, and test set.
3. Use the training set to calculate the emperical mean and standard deviation for each channel of the samples.
4. Update Training/SMIL.py with calculated channel mean and standard deviations.

1. For training the network based on a new dataset, modify the data loader to properly accomodate your needs. This mostly consists of setting the number of input channels in the sample images, the transfomations required to be performed on the images, meta data required to be stored for each instance, and the patch selection mechanism.
2. Define a deep neural network along with the loss function suitable for the task at hand.
3. Then run the Training/SMIL.py which trains a model based on ResNet50 and logs the training process in convergence.csv. The best model based on the validation set, if presented, is stored at checkpoint_best.pth.

Provide proper arguments for Analysis/analyze.py and run it. It will provide you with the predicted infection probabilities for each patch in h5 format, estimated infection probabilities in .csv which is later used for calculating treatment efficacy scores. It will also generates performance report for each split provided.

Use the .csv file generated in inference to run the Analysis/Analysis.R. It will generate a ranked list of treatments along with their estimated efficacy score in .csv.

@ARTICLE{tmi2022,
   AUTHOR       = {M.Sadegh Saberian and Kathleen Moriarty and 
      Andrea Olmstead and Christian Hallgrimson and François Jean and 
      Ivan Robert Nabi and Maxwell Libbrecht and Ghassan Hamarneh},
   JOURNAL      = {IEEE Transactions on Medical Imaging (IEEE TMI)},
   TITLE        = {DEEMD: Drug Efficacy Estimation against SARS-CoV-2 based 
      on Cell Morphology with Deep Multiple Instance Learning.},
   YEAR         = {2022},
   DOI          = {10.1109/TMI.2022.3178523},
   KEYWORDS     = {Deep Learning, Machine Learning, COVID19, SARS-CoV-2},
   PDF          = {http:https://www.cs.sfu.ca/~hamarneh/ecopy/tmi2022.pdf},
}

If you found the content of this repository useful, please consider citing our work:

PDF: https://www.cs.sfu.ca/~hamarneh/ecopy/tmi2022.pdf

DOI: http:https://dx.doi.org/10.1109/TMI.2022.3178523

PubMed: https://pubmed.ncbi.nlm.nih.gov/35622798