Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

0.38.0 dev #72

Merged
merged 4 commits into from
Jun 15, 2024
Merged
Show file tree
Hide file tree
Changes from 1 commit
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
Prev Previous commit
Next Next commit
Deprecate the common.plot_cytobands method (#71)
  • Loading branch information
sbslee committed Jun 15, 2024
commit 0f4c601eb8c9612260a01bcbd02e62513b852bfd
1 change: 1 addition & 0 deletions CHANGELOG.rst
Original file line number Diff line number Diff line change
Expand Up @@ -5,6 +5,7 @@ Changelog
-----------------------

* Update :meth:`pyvcf.has_chr_prefix` method to ignore the HLA contigs for GRCh38.
* :issue:`71`: Deprecate :meth:`common.plot_cytobands` method.

0.37.0 (2023-09-09)
-------------------
Expand Down
133 changes: 0 additions & 133 deletions fuc/api/common.py
Original file line number Diff line number Diff line change
Expand Up @@ -22,7 +22,6 @@
import pandas as pd
import numpy as np
import matplotlib.pyplot as plt
from matplotlib.collections import BrokenBarHCollection
import matplotlib.patches as mpatches
import seaborn as sns

Expand Down Expand Up @@ -841,138 +840,6 @@ def extract_sequence(fasta, region):
sequence = ''
return sequence

def plot_cytobands(cytoband, bed, ax=None, figsize=None):
"""
Create chromosome ideograms along with BED data.

The method's source code is derived from a Python script (ideograms.py)
written by Ryan Dale. The original script can be found at:
https://gist.github.com/daler/c98fc410282d7570efc3#file-ideograms-py

Parameters
----------
cytoband : str
Text file containing cytoband ideogram information.
bed : str
BED file to be displayed.
ax : matplotlib.axes.Axes, optional
Pre-existing axes for the plot. Otherwise, crete a new one.
figsize : tuple, optional
Width, height in inches. Format: (float, float).

Examples
--------

.. plot::
:context: close-figs

>>> import matplotlib.pyplot as plt
>>> from fuc import common
>>> common.load_dataset('cytoband')
>>> cytoband_file = '~/fuc-data/cytoband/cytoBandIdeo.txt.gz'
>>> bed_file = '~/fuc-data/cytoband/ucsc_genes.bed.gz'
>>> common.plot_cytobands(cytoband_file, bed_file, figsize=(10, 8))
"""
def chromosome_collections(df, y_positions, height, **kwargs):
del_width = False
if 'width' not in df.columns:
del_width = True
df['width'] = df['end'] - df['start']
for chrom, group in df.groupby('chrom'):
yrange = (y_positions[chrom], height)
xranges = group[['start', 'width']].values
yield BrokenBarHCollection(
xranges, yrange, edgecolors=("black",), facecolors=group['colors'], **kwargs)
if del_width:
del df['width']

# Height of each ideogram
chrom_height = 1

# Spacing between consecutive ideograms
chrom_spacing = 1

# Height of the gene track. Should be smaller than `chrom_spacing` in order to
# fit correctly
gene_height = 0.4

# Padding between the top of a gene track and its corresponding ideogram
gene_padding = 0.1

# Decide which chromosomes to use
chromosome_list = [f'chr{i}' for i in list(range(1, 23)) + ['M', 'X', 'Y']]

# Keep track of the y positions for ideograms and genes for each chromosome,
# and the center of each ideogram (which is where we'll put the ytick labels)
ybase = 0
chrom_ybase = {}
gene_ybase = {}
chrom_centers = {}

# Iterate in reverse so that items in the beginning of `chromosome_list` will
# appear at the top of the plot
for chrom in chromosome_list[::-1]:
chrom_ybase[chrom] = ybase
chrom_centers[chrom] = ybase + chrom_height / 2.
gene_ybase[chrom] = ybase - gene_height - gene_padding
ybase += chrom_height + chrom_spacing

# Read in ideogram.txt, downloaded from UCSC Table Browser
ideo = pd.read_table(
cytoband,
names=['chrom', 'start', 'end', 'name', 'gieStain']
)

# Filter out chromosomes not in our list
ideo = ideo[ideo.chrom.apply(lambda x: x in chromosome_list)]

# Add a new column for width
ideo['width'] = ideo.end - ideo.start

# Colors for different chromosome stains
color_lookup = {
'gneg': (1., 1., 1.),
'gpos25': (.6, .6, .6),
'gpos50': (.4, .4, .4),
'gpos75': (.2, .2, .2),
'gpos100': (0., 0., 0.),
'acen': (.8, .4, .4),
'gvar': (.8, .8, .8),
'stalk': (.9, .9, .9),
}

# Add a new column for colors
ideo['colors'] = ideo['gieStain'].apply(lambda x: color_lookup[x])

# Same thing for genes
genes = pd.read_table(
bed,
names=['chrom', 'start', 'end', 'name'],
usecols=range(4))
genes = genes[genes.chrom.apply(lambda x: x in chromosome_list)]
genes['width'] = genes.end - genes.start
genes['colors'] = '#2243a8'

if ax is None:
fig, ax = plt.subplots(figsize=figsize)

# Now all we have to do is call our function for the ideogram data...
for collection in chromosome_collections(ideo, chrom_ybase, chrom_height):
ax.add_collection(collection)

# ...and the gene data
for collection in chromosome_collections(
genes, gene_ybase, gene_height, alpha=0.5, linewidths=0
):
ax.add_collection(collection)

# Axes tweaking
ax.set_yticks([chrom_centers[i] for i in chromosome_list])
ax.set_yticklabels(chromosome_list)
ax.axis('tight')

return ax

def convert_file2list(fn):
"""
Convert a text file to a list of filenames.
Expand Down