R package for performing cell type deconvolution of bulk RNA sequencing, single cell RNA sequencing, and bisulfite sequencing data.

The user can download the tar ball, untar the tarball, and use the devtools package to install dependencies then run R CMD INSTALL on the command line:

#in R
library(devtools)
install_deps("/PATH/TO/deconvSeq",dependencies=TRUE)
#on command line
R CMD INSTALL deconvSeq_VERSION_NUMBER.tar.gz

Alternatively, use the devtools package to install from GitHub:

## devtools is required
library(devtools)
install_github("rosedu1/deconvSeq", dependencies=TRUE)

Note:

1. Windows users need Rtools and devtools to install from GitHub.
2. If some packages do not install, set repositories to include both CRAN and Bioconductor with setRepositories(ind=c(1:4)).

Vignette: HTML Vignette

8/16/20 version 0.3.0 1) Added bismark filetypes to getmethmat.

6/17/20 version 0.2.5 1) Reduced vignette run time

5/25/20 version 0.2.4 1) Added citation to vignette and package

5/25/20 version 0.2.3 1) Commented out time consuming portions of vignette and added precompiled data

5/25/20 version 0.2.2 1) Removed examples from getcorr

5/23/20 version 0.2.1 1) Removed dependency on simpleSingleCell from package

5/21/20 version 0.2.0 1) Reduced size of data_scrnaseq.rda to reduce package size. 2) Updated prep_scrnaseq to include error message for wrong genenametype.

5/20/20 version 0.1.4 1) Updated mydiffMethFromDesign_matrix.R, line 169, class(res)=="list" changed to class(res)[1]=="list". This does not affect the functionality of the package.

5/20/20 version 0.1.3 1) in withDoc.R, function prep_scrnaseq, replaced defunct functions calculateQCMetrics with perCellQCMetrics and replaced defunct calcAverage with calculateAverage. 2) in function getmethmat, added filetypes to include output from Bismark as well as BSMAP, 3) in function getmethmat, added condition to not filter if there are no cases where all Cs or all Ts are 0.

Du R, Carey V, and Weiss ST. deconvSeq: deconvolution of cell mixture distribution in sequencing data, Bioinformatics, 35:5095-5102, 2019.

You are welcome to:

• submit suggestions and bug-reports at: https://github.com/rosedu1/deconvSeq/issues
• email: [email protected]