Script for generating structures of two-tailed primers from imported sequences.

Program will connect to https://eu.idtdna.com/calc/analyzer and click hairpin button for each sequence. All the images will be written to an output HTML file that will be displayed in the browser.

Python3 + Selenium (Chrome) application are prerequisited.

1. Download https://sites.google.com/a/chromium.org/chromedriver/downloads
2. pip3 install git+https://github.com/raradek/oligo_analyzer_hairpin.git --user OR download this dir and launch pip3 install -r requirements.txt
3. Put chromedriver.exe in the same path as OligoAnalyzer.py
4. Optionally put your user name and pw to user.ini along with other parameters
5. Execute the OligoAnalyzer.py
6. You will be asked for data repersenting the sequences to be analyzed. Copy there ex. these columns from the Excel:

Name:Sequence
test1:AGAGAGAGAGCCC
test2:AGAGAGAGAGCCCTATAGAGAGAGAGAGCCCTTTTT
test3:AGAGAGAGAGCCCTAGAGAGAGAGAGAG
test4:AGAGAGAGAGAGCCCCCCCTTTT
test5:AGAGAGAGAGAGAGCCCCCC
test6:CCCTTTTTTAAGAGAGAG
test7:AGAGAGAGAGCCCCTAGAGAGAGAGAGAGGGGGGG
test8:GGGAGAGAGAGAGAGAGAGAGAGAG
test9:TAGAGAGAGAGAGAGAGAGAGAG
test10:ACCCCTAGAGAGAGAGAGAGAGAG

(note that each line is in format: name:sequence)