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SeqSizzle is a pager for viewing FASTQ files with fuzzy matching, allowing different adaptors to be colored differently.
Latest pre-built release binary

Usage

./SeqSizzle -h:

Usage: SeqSizzle [OPTIONS] <FILE>

Arguments:
  <FILE>  The FASTQ file to view

Options:
      --adapter-3p
          Start with 10x 3' kit adaptors:
           - Patrial Read1: CTACACGACGCTCTTCCGATCT (and reverse complement)
           - Partial TSO: AGATCGGAAGAGCGTCGTGTAG (and reverse complement)
           - Poly(>10)A/T
      --adapter-5p
          Start with 10x 5' kit adaptors
           - Patrial Read1: CTACACGACGCTCTTCCGATCT (and reverse complement)
           - TSO: TTTCTTATATGGG (and reverse complement)
           - Patrial Read2: AGATCGGAAGAGCACACGTCTGAA (and reverse complement)
           - Poly(>10)A/T
  -p, --patterns <PATTERNS_PATH>
          Start with patterns from a CSV file
          Must have the following header:
          pattern,color,editdistance,comment
  -s, --save-patterns <SAVE_PATTERNS_PATH>
          Save the search panel to a CSV file before quitting. 
          To be removed in the future since you can now hit Ctrl-S in 
          the search panel to save the patterns
  -h, --help
          Print help
  -V, --version
          Print version

Navigation

Viewer mode

Viewer mode Up / down arrow (or j / k) to scroll by one line, Ctrl+U / Ctrl+D to scoll half a screen.
/ (or Ctrl+F) to toggle search panel, q to quit

search panel mode

Search panel mode Left / right arrow (or Tab / Shift-Tab) to cycle through different input fields and the patterns list.
When on the patterns list field, up / down arrows cycle through patterns, Backspace (or Delete, d) to delete the selected pattern and Return to pop the pattern into the input fields for editing.
Return to add current inputs into the search pattern list (when focusing on any of the input boxes, rather than the patterns list).
Use Shift + arrow keys to move cursor within an input field (as arrow keys alone are bind to cycling input fields).
/ or Esc to close the search panel.

Roadmap

functionality

  • Gzip (fastq.gz) support
  • Filter reads by match
  • Counting reads with match

UI

  • Make elements in the search panel clickable, try implementations discussed in ratatui repo

Misc

  • Unit tests

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  • Rust 100.0%