GitHub - luksgrin/TmBioPHP-python: Tm calculation considering Base Stacking. It is a translated code from the original php code made by Dr. Joseba Bikandi, lecturer at the University of Basque Country (Spain). Original source code available at https://www.biophp.org/minitools/melting_temperature/). The following references were used to develop the script: -SantaLucia J. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1460-5. https://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=9465037-von Ahsen N, Oellerich M, Armstrong VW, Sch�tz E. Application of a thermodynamic nearest-neighbor model to estimate nucleic acid stability and optimize probe design: prediction of melting points of multiple mutations of apolipoprotein B-3500 and factor V with a hybridization probe genotyping assay on the LightCycler. Clin Chem. 1999 Dec;45(12):2094-101. https://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=10585340

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Tm calculation considering Base Stacking. It is a translated code from the original php code made by Dr. Joseba Bikandi, lecturer at the University of Basque Country (Spain). Original source code available at https://www.biophp.org/minitools/melting_temperature/). The following references were used to develop the script: -SantaLucia J. A unified …