An R package version of interactive differential expression analyzer
High-throughput sequencing technology is rapidly becoming the standard method for measuring gene expression at the transcriptional level. One of the main goals of such work is to identify differentially expressed genes under two or more conditions. A number of computational tools , such as DESeq (Anders and Huber 2010) (updated as DESeq2 (Love, Huber et al. 2014)), edgeR (Robinson, McCarthy et al. 2010, Zhou, Lindsay et al. 2014), NOISeq (Tarazona, García-Alcalde et al. 2011), PoissonSeq (Li, Witten et al. 2011), and SAMseq (samr) (Li and Tibshirani 2013) and Cuffdiff (Trapnell, et al., 2013) have been developed for the analysis of differential gene expression from patterns in RNA-seq data. Most of these tools are implemented in R language, which is commonly used for the analysis of high-dimensional expression data. However, a fairly high level of programing skill is required when applying these R tools to screen out differentially expressed genes, greatly hindering the application of these tools since many biology researchers have little programing experience. Beyond this problem, due to a lack of an interactive interface in these tools, it is inconvenient to adjust the analytical parameters, even for advanced users. Moreover, since different packages generate inconsistent results, an interactive platform that combines these tools together is necessary for obtaining more solid analysis results.
To address the above issues, here we introduce the Interactive Differential Expression Analyzer (IDEA), a Shiny-based web application dedicated to the identification of differential expression genes in an interactive way. IDEA was built as a user-friendly and highly interactive utility using the Shiny (RStudio Inc. 2014) package in R. Currently, five relevant R packages are integrated into IDEA. IDEA is capable of visualizing the results with plenty of charts and tables, as well as providing great ease of interaction during the course of the analysis.
Click me to redirected into web server.
Screen shoot of IDEA
The online version can be easily accessed to perform analysis tasks with dataset in low thoughput. However, Limited to remote server resouces, the web page usually stucked while multible user operated parallelly or larger dataset tasks requirement. To solve this, We released an R package that packed the core analysis module for users to run analysis under the R environment locally .
Before install the IDEA package, please read this markdown file carefully;
if (!require("devtools"))
install.packages("devtools")
devtools::install_github("likelet/IDEA