Funded by USDA NRSP-8 Aquaculture small project grant
Location: URI Alton Jones Campus Whispering Pines
-
Annotating Epigenetic features in the Eastern Oyster Genome (EOG)
- DNA methylation
- Chromatin associated proteins
- Transposable Elements
- miRNA
- lncRNA
-
Comparison of DNA methylation quantification methods in the EOG
- WGBS - Whole Genome Bisulfite Sequencing
- MBD-BS - Methyl Binding Domain enrichment followed by bisulfite sequencing
- MSPI - Reduced representation digestions with MSPI
-
Annotation for EOG and gap analysis for improved epigenetics annotation approaches
-
Manuscript on methods comparison for DNA methylation data - target: Frontiers in Marine Science Special Issue: Marine Environmental Epigenetics
- Hollie Putnam - URI
- Steven Roberts - UW
- Jose Eirin-Lopez - FIU
- Kevin Johnson - LSU
- Sam Gurr - URI
- Jon Puritz - URI
- Katie Lotterhos - NEU
- Alan Downey Wall - NEU
- Bodie Weedop - NEU
- Yaamini Venkataraman - UW
- Mackenzie Gavery - NOAA
- Shelly Trigg - UW
- Qiagen Data (method comparison)
- LSU Oil Exposure Data
- Resequencing Data
May 20th Arrive PVD in the afternoon and be transported to Hampton Inn
- Dinner as at group meet in Lobby at 5:45pm
May 21st 08:00 meet in parking lot for transport to URI Alton Jones Campus Whispering Pines
- Breakfast at the hotel
09:30 - Introductions, Annotation Data resources
11:00 coffee break
11:15 - 12:30 - Data Overview and Gap analysis
12:30 Lunch
1:30 - 3:15 - Annotation work
3:15 Coffee Break
3:30 - 5:00 Annotation work and summary of day
5:00 - 5:30 Introduction to data for day 2 methods comparison
6:00 dinner
08:30 - Overview for day 2 methods comparison
09:30 - 11:00 - Data Analysis and methods writing
11:00 coffee break
11:15 - 12:30 - Data Analysis and methods writing
12:30 Lunch
1:30 - 3:15 - Annotation work
3:15 Coffee Break
3:30 - 4:30 Data Analysis and methods writing
4:30 - 5:30 Summary of progress for day 2, Setting goals and timelines for two products
6:00 dinner
08:30 - Overview for sections and timeline
09:30 - 11:00 - Breakout Groups
11:00 coffee break
11:15 - 12:30 - Breakout Groups and summary
12:30 Lunch
Departure or continue on to Genomics workshop