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Take Olympus vsi stacks. Bleach-correct, write out ome-tiff. Save clicks.

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vsi2tif

Take Olympus vsi stacks. Bleach-correct, write out ome-tiff, possibly by channel. Save clicks.

Tested for 3D XYZ Data, but not for timeseries.

Bleach-correction by histogram matching, according to Miura (2020). Implementation inspired by napari-bleach-correct.

mamba create -n vsi2tif python=3.10 bioformats_jar -c conda-forge
mamba activate vsi2tif
pip install "git+https://github.com/bwmr/vsi2tif.git"
vsi2tif --help
vsi2tif session_folder/*vsi

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Take Olympus vsi stacks. Bleach-correct, write out ome-tiff. Save clicks.

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