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microarray-analysis

Materials on the analysis of microarray expression data; focus on re-analysis of public data

Day one

  • Intro to microarray technology (Illumina / affy) & Data analysis

    • probes, target terminology
    • Bioconductor introduction
  • Prac; 1st look at microarray data and R refresher

    • Existing Bioc practical

-Specific technologies + Affy workflow + Illumina workflow + Diagnostic plots + Quality control / normalisation

  • Linear models and statistics for differential expression

Day two

  • Practical on differential expression

  • Advanced aspects of analysis

    • Annotation
    • Batch effects
  • Clustering, PCA, (survival?)

  • Existing prac

Use this?

source("https://bioconductor.org/biocLite.R") biocLite("Affyhgu133Plus2Expr")