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fixed readme
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bhofmei committed Mar 16, 2017
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#Nucleotide Density Plugin
# Nucleotide Density Plugin

This is a JBrowse plugin
This is a JBrowse plugin.

Allows the user to create multi-lined heatmaps for different nucleotide contexts. User can set the color for each context, a single color for all contexts, or "random" colors will be chosen (see [below]).
##Special Thanks
Allows the user to create multi-lined heatmaps for different nucleotide contexts. User can set the color for each context, a single color for all contexts, or "random" colors will be chosen (see [below](#color-parameters)).
## Special Thanks
Special thanks to [Colin Diesh](http:https://cmdcolin.github.io/) who inspired components of this plugin. Particularly, the [GC Content plugin](https://github.com/elsiklab/gccontent) (for the storage class) and [Multi BigWig plugin](https://github.com/elsiklab/multibigwig) (for multiple density tracks).

##Install
"Random" color generation was possible thanks to [Chroma.js]
(https://github.com/gka/chroma.js/). A copy downloaded March 16, 2017 is included in this plugin.

## Install

For JBrowse 1.11.6+ in the _JBrowse/plugins_ folder, type:
`git clone https://github.com/bhofmei/jbplugin-nucdens.git NucleotideDensityPlugin`
Expand All @@ -33,8 +36,8 @@ If that doesn't work, add this to _jbrowse_conf.json_:
}
```

##Using Nucleotide Density Traacks
###Example
## Using Nucleotide Density Traacks
### Example
{
"key" : "Nucleotide Density",
"label" : "nuc_dens",
Expand All @@ -46,7 +49,7 @@ If that doesn't work, add this to _jbrowse_conf.json_:
Sequence contexts must be specified in an array.
[Degenerate/IUPAC](http:https://www.bioinformatics.org/sms/iupac.html) nucleotides are supported. Gaps are not. Nucleotides can be lowercase or uppercase.

###Color Parameters
### Color Parameters
For all contexts, density is indicated by the intensity of color. Color is computed as a blend between the background color and specified color weighted by the scaled density.
Density is scaled to the track configuration `min_score` and `max_score`.

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![Example for color parameters](img/example_colors.png)

###Addititional Parameters
### Addititional Parameters
Additional parameters that can be specified for the track configuration.
* `"min_score" : 0` , minimum density when computing color
* `"max_score" : 1` , maximum density when computing color
Expand All @@ -90,6 +93,8 @@ Additional parameters that can be specified for the track configuration.
* `"style" : { "height" : 70 }` , track height


##Future Plans
## Future Plans
- "Loading" screen when recomputing after changing a track setting
- turn on/off labels using CSS without redrawing
- Ability to add track locally by adding option to menu bar, "File"
- Possibly improved score view (currently works fine but is not visually appealing)

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