Project to simulate simple Oxford Nanopore Technologies RNA signals
The signal gets simulated by providing a reference sequence or generating a random reference sequence.
We use Gaussian signal distributions provided by ONT to simulate the signal for each 5-mer in the reference sequence.
We analyzed the segment distribution on IVT RNA data sequenced with ONT. The segment lengths were calculated by ONTs software nanopolish eventalign. We fitted negative binomial distributions on each 5-mer length distribution and use them to simulate segment lengths with ont_simig.
To change the models used for the simulation you have to change the content of the files linked above.