A python-based software package targeted for analyzing Cytof images captured by the Hyperion™ imaging system.

Claim: the full repo of the package is still protected and released. Only main ideas and visualization is presented here. Accessing the repo is upon request through email.

IMC (Imaging Mass Cytometry):

• A powerful high-throughput technique enabling resolution of up to 37 markers in a fixed tissue section, with the spatial relationships preserved simultaneously.
• This next-generation technology relies on the general principles of IHC (immunohistochemistry) but instead uses antibodies conjugated to rare earth metals with a discrete mass.
• Combines IHC (immunohistochemistry) staining using metal isotope-labelled antibodies with laser ablation and mass-spectrometry-based detection to produce high-dimensional images
• The resulting image is a multi-spectral image of a tissue, where each channel represents for a specific marker.

ROI: an area on the tissue sample that is determined prior to data acquisition and is imaged by the Hyperion™ system.