BAS is a Python-based program for exploratory analysis of digital images from biofilm growth experiments.

Download here the .exe file that corresponds to your OS.

You should always arrange your images from experiments in 2 folders: Biofilm and Reference. This will allow the program to identify them and function correctly.

Typical usage should be as follows:

1. Run the .exe file
2. Select the experiment folder
3. Follow the steps inside the UI
4. Export your results

Detailed user instructions can be found here (only in Spanish)

• Image segmentation
• Filter selection: Grayscale and Green channel
• Biofilm quantification based on a Thresholding Method
• Descriptive Statistics of Biofilm images

If you find this project useful, please cite this article: Castilla-Sedano, A. J., Zapana-García, J., Valdivia-Del Águila, E., Padilla-Huamantinco, P. G., & Guerra, D. G. (2024). Quantification of early biofilm growth in microtiter plates through a novel image analysis software. Journal of Microbiological Methods, 106979–106979. https://doi.org/10.1016/j.mimet.2024.106979