After all subjects were euthanized, incisions were made at recipient site, dissection was performed, and fat grafts were accessed through panniculus tissue.
A full thick excision wounds of 5 mm in diameter were made on the dorsal skin (including epidermis, dermis, sub cutaneous fat and the underlying panniculus carnosus) using a sterile biopsy punch (Chen et al., 2015).
The worst place for such incisions is in the crease above the pubis and below the panniculus. Invariably, this area remains moist and macerated, especially in obese patients, thus providing a fertile breeding ground for bacteria.