Also, neuroendocrine tumors would be typically negative for cytokeratin 7,
inhibin, and calponin.
Serum levels of follicle-stimulating hormone, oestradiol and
inhibin B to measure ovarian reserve have the limitation of being cycle dependent.
To increase fecundity in sheep, many strategies have been followed such as genetic methods including selection of breeding programs, and non-genetic methods including administration of exogenous gonadotropins such as equine chorionic gonadotropin (eCG) [5], and, recently, immunization against endogenous
inhibin [6].
Blood sample for serum human chorionic gonadotropin (HCG) was analyzed on random access immulite 2000, alpha-fetoprotein (AFP) was analyzed on ADVIA Centaur, unconjugated estriol (uE3) and
Inhibin A measured by Enzyme-Linked Immunosorbent Assay (ELISA) method by PR 4100 Micro plate Reader.
Serum levels of AMH (AMH Gen II ELISA, Beckman Coulter Inc., USA) and
inhibin B (
Inhibin B Gen II ELISA, Beckman Coulter Inc., USA) were <1.0 pmol/L (reference interval: 7.1-71.4 pmol/L) and 30.2 pg/mL (reference interval: 10-273 pg/mL), respectively.
Inhibiting the alpha subunit of
inhibin (INHA), which is related to infertility (Rah et al., 2014) and ovarian insufficiency (Li et al., 2015).
Immunohistochemical evaluation revealed diffuse positive staining with smooth muscle actin and vimentin, and negative staining with desmin,
inhibin, cytokeratin and S-100 protein in the tumor (Figure 1f).
Hyperoestrogenism reported in patients with GCT is related to tumor production of oestrogens, anti-Mullerian hormone, and
inhibin B [4].
Blood work revealed elevated carbohydrate antigen (CA) 125 and positive QuantiFERON-TB Gold, but adenosine deaminase, CA 19, alpha-fetoprotein, and
inhibin B were within normal limits.
On immunohistochemistry, neoplastic cells from the large mass were positive for CD117 (Figure 2(a)), placental alkaline phosphatase (PLAP) (Figure 2(b)), and CD10 and negative for
inhibin (Figure 2(c)), cytokeratin (Figure 2(d)), [beta]-catenin, smooth muscle actin (SMA), synaptophysin, desmin, S100, [beta]-HCG, and [alpha]-fetoprotein.
The immunohistochemical markers for calretinin and
inhibin were negative (Table 1).
Inhibin and other sex hormone serologies were not performed preoperatively.