Ioannis Therios Olives

CROP PRODUCTION SCIENCE IN HORTICULTURE
SERIES
Series Editors: Jeff Atherton, Professor of Tropical
Horticulture, University of the West Indies, Barbados, and
Alun Rees (retired) former Head of Crop Science, Glasshouse
Crops Research Institute, Horticultural Consultant and Editor
of the Journal of Horticultural Science and Biotechnology.
This series examines economically important horticultural crops selected from
the major production systems in temperate, subtropical and tropical climatic
areas. Systems represented range from open eld and plantation sites to
protected plastic and glasshouses, growing rooms and laboratories. Emphasis is
placed on the scientic principles underlying crop production practices rather
than on providing empirical recipes for uncritical acceptance. Scientic
understanding provides the key to both reasoned choice of practice and the
solution of future problems.
Students and staff at universities and colleges throughout the world involved
in courses in horticulture, as well as in agriculture, plant science, food science
and applied biology at degree, diploma or certicate level, will welcome this
series as a succinct and readable source of information. The books will also be
invaluable to progressive growers, advisers and end-product users requiring an
authoritative, but brief, scientic introduction to particular crops or systems. Keen
gardeners wishing to understand the scientic basis of recommended practices
will also nd the series very useful.
The authors are all internationally renowned experts with extensive
experience of their subjects. Each volume follows a common format covering all
aspects of production, from background physiology and breeding, to propagation
and planting, through husbandry and crop protection, to harvesting, handling and
storage. Selective references are included to direct the reader to further
information on specic topics.
Titles Available:
1. Ornamental Bulbs, Corms and Tubers A.R. Rees
2. Citrus F.S. Davies and L.G. Albrigo
3. Onions and Other Vegetable Alliums J.L. Brewster
4. Ornamental Bedding Plants A.M. Armitage
5. Bananas and Plantains J.C. Robinson
6. Cucurbits R.W. Robinson and D.S. Decker-Walters
7. Tropical Fruits H.Y. Nakasone and R.E. Paull
8. Coffee, Cocoa and Tea K.C. Willson
9. Lettuce, Endive and Chicory E.J. Ryder
10. Carrots and Related Vegetable Umbelliferae V.E. Rubatzky, C.F. Quiros
and P.W. Simon
11. Strawberries J.F. Hancock
12. Peppers: Vegetable and Spice Capsicums P.W. Bosland and E.J. Votava
13. Tomatoes E. Heuvelink
14. Vegetable Brassicas and Related Crucifers G. Dixon
15. Onions and Other Vegetable Alliums, 2nd Edition J.L. Brewster
16. Grapes G.L. Creasy and L.L. Creasy
17. Tropical Root and Tuber Crops: Cassava, Sweet Potato, Yams and
Aroids V. Lebot
18. Olives I. Therios
This page intentionally left blank
OLIVES
Ioannis Therios
School of Agriculture
Aristotle University
Thessaloniki
Greece
CABI is a trading name of CAB International

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Ioannis Therios 2009. All rights reserved. No part of this

publication may be reproduced in any form or by any means,
electronically, mechanically, by photocopying, recording or
otherwise, without the prior permission of the copyright owners.
A catalogue record for this book is available from the British Library,
London, UK.
Library of Congress Cataloging-in-Publication Data
Therios, Ioannis Nikolaos.
Olives / I. Therios.
p. cm. -- (Crop production science in horticulture ; 18)
ISBN 978-1-84593-458-3 (alk. paper)
1. Olive. I. Title. II. Series.
SB367.T44 2009
634.63--dc22
2008015354
ISBN-13: 978 1 84593 458 3
Typeset by Columns Design, Reading, UK.
Printed and bound in the UK by Biddles Ltd, Kings Lynn.
CONTENTS
PREFACE
ix
ACRONYMS AND ABBREVIATIONS
xi
CHEMICAL SYMBOLS
xv
1
HISTORY OF OLIVE GROWING
2
THE OLIVE: ORIGIN AND CLASSIFICATION
3
MORPHOLOGY AND TAXONOMY OF THE OLIVE
13
4
STRUCTURE AND COMPOSITION OF THE OLIVE FRUIT
25
5
HECTAREAGE, NUMBER OF TREES AND PRODUCTION OF
OLIVE OIL AND TABLE OLIVES
33
6
ROOTSTOCKS
43
7
MAJOR TRENDS IN OLIVE FARMING SYSTEMS
49
8
CLIMATIC AND SOIL CONDITIONS
51
v
vi
Contents
9
FLOWER BUD INDUCTION AND DIFFERENTIATION
81
10
FLOWERING, POLLINATION, FERTILIZATION AND FRUITING
93
11
ALTERNATE BEARING
105
12
FRUIT THINNING
109
13
SYSTEMS OF PLANTING AND CANOPY TRAINING
113
14
PROPAGATION OF OLIVE TREES
125
15
IRRIGATION OF THE OLIVE
151
16
WATER USE EFFICIENCY BY THE OLIVE
167
17
STRESS-INDUCED ACCUMULATION OF PROLINE AND MANNITOL
175
18
MINERAL NUTRITION OF THE OLIVE
179
19
GROWTH AND SALT TOLERANCE OF THE OLIVE
211
20
PRUNING
221
21
OLIVE RIPENING
229
22
OLIVE FRUIT HARVESTING
245
Contents
vii
23
OLIVE VARIETIES
255
24
TABLE OLIVES
271
25
OLIVE OIL
279
26
OLIVE MILL PRODUCTS AND ENVIRONMENTAL IMPACT OF
OLIVE OIL PRODUCTION
295
27
OLEUROPEIN, OLIVE LEAF EXTRACT, OLIVE OIL AND THE
BENEFITS OF THE MEDITERRANEAN DIET TO HUMAN HEALTH
303
28
BIOLOGICAL AND INTEGRATED OLIVE CULTURE
319
29
CHEMICAL AND INTEGRATED WEED MANAGEMENT IN OLIVE
ORCHARDS
325
30
PESTS AND DISEASES
335
31
BIOTECHNOLOGICAL ASPECTS OF OLIVE CULTURE
353
REFERENCES
357
INDEX
399
PREFACE
Olive culture is rapidly growing in terms of both production volume and

geographical spread. The crop is now grown in countries not traditionally
associated with its cultivation, although in such countries olive culture is not
yet economically signicant. Furthermore, climatic changes have resulted in
the expansion of olive culture to areas more northerly than the Mediterranean
countries. The olive is not only a signicant food source but it also contributes
to human health, with olive products representing an important part of the
Mediterranean diet, which an increasing number of people all over the world
are embracing for reasons of health.
This book of olives is the result of many years endeavours in collecting
valuable information from the existing literature concerning the olive tree and its
culture; a proportion of this information, and experience, has originated from
scientic projects of the author and his scientic team. Topics include all aspects
of olive culture, from its history, through traditional practices to modern
techniques and horticultural procedures. Furthermore, this book covers the basic
physiological and horticultural principles of olive culture in both theory and
practice.
My objective is to provide knowledge appropriate for students, scientists,
both experienced and inexperienced horticulturists and, in general, for
anyone wishing to obtain knowledge and experience of olive culture in order
to increase productivity and improve product quality. The content is written
in a style accessible to anyone and is also appropriate as a textbook at various
levels of education. To enhance understanding the text includes illustrations,
gures and tables featuring experimental data, and it is a recent addition to
the literature on olives.
Ioannis Therios
Professor of Pomology
Aristotle University
Thessaloniki
Greece
ix
ACRONYMS AND ABBREVIATIONS
2, 4, 5-T
2, 4-D
2iP
3-CPA
ABA
ACC
ACE
ADP
AFLP
AMP
AOS
ATP
BA
BAP
BMI
BN
BNOA, NOA
CAP
CBF1
CCC
CEC
CHA
CK
CLRV
CMS
COR
CTP
CuMV
DNA
DNOC
2, 4, 5-trichlorophenoxyacetic acid
2, 4-Dichlorophenoxyacetic acid
6-(, -dimethylallylamino)-purine
3-chlorophenoxy--propionamide
Abscisic acid
1-aminocyclopropane-1 carboxylate
Acetylcholinesterase
Adenosine diphosphate
Amplied fragment length polymorphism
Adenosine monophosphate
Active oxygen species
Adenosine triphosphate
6-benzylamino purine or benzyladenine
Benzylamino purine
Body mass index
Nutrient medium
-naphthoxyacetic acid
Common agricultural policy
Regulator of cold acclimation
(2-chloroethyl)trimethylammonium chloride or
Chlormequat
Cation exchange capacity
Chlorogenic acid
Cytokinin
Cherry leaf roll virus
Cytoplasmic male sterility
Cold Regulated protein
Cytidine triphosphate
Cucumber mosaic virus
Deoxyribonucleic acid
4, 6-dinitro-ortho-cresol
xi
xii
DNP
DTA
ECe
ECw
EDTA
ERD
ETc'
Ethephon
Ethrel
ETo
FASN
FDF
Fenoprop
FMN
FTIR spectroscopy
Fts
Fv
GA3
GA4
GAs
GC
GDH
GGA
gMS
GOGAT
GS
gs
HER2
HPLC
Hsp
IAA
IBA
iNOs
ISSR
Kc
KGA3
KIN
KIN
KMnO4
Kp'
KS1
LAI
LAR
Acronyms and Abbreviations
2, 4- Dinitrophenol
Differential thermal analysis
Electrical conductivity
Electrical conductivity of irrigation water
Ethylenediamine tetraacetic acid
Early responsive to dehydration
Actual water requirements
2-chloroethyl phosphonic acid
see Ethephon
Potential evapotranspiration
Fatty acid synthase (human gene)
Fruit detachment force
see 2, 4, 5-TP
Flavin mononucleotide
Fourier transform infrared spectroscopy
Filamentous temperature sensitive proteins
Variable uorescence
Gibberellic acid
Gibberellin
Gibberellins
Gas chromatography
Glutamic dehydrogenase
2-chloroethyl-tris-2-methoxy-ethoxy silane or Alsol
Genic male sterility
Glutamate-2-oxoglutarate amido transferase
Glutamic synthetase
Leaf conductance
Cancer genes
High pressure liquid chromatography
Heat stress proteins
Indole-3-acetic acid
Indole-3-butyric acid
Inductible nitric oxide synthase
Inter-simple sequence repeats
Crop coefcient
Potassium gibberellate
6-furfurylamino purine, syn N-furfuryladenine or
Kinetin
Cold induce
Potassium permanganate
Coefcient related to tree transpiration
Coefcient related to soil evaporation
Leaf area index
Leaf area ratio
LC
LDL
LEA
LFDI
LLC
L-Name
LOX
LPS
LSC
LT1
LVI
M
MI
MH
mOM
mRNA
MS medium
MSMA
MYB
N
NAA
NAAm
NADP
NADPH
NAR
NIF
NMR
NPA
NR
OD
OLE
Oleaster
OM
OMWW
OP
PAR
PCD
PDO
PEA 3
PEP
PGI
Pn
PPF
xiii
Liquid chromatography
Low density lipoprotein
Late embryogenesis abundant proteins (prevent protein
aggregation)
Low frequency decit irrigation
Liquidliquid chromatography
N(G)nitro-L-arginine methyl ester
Lipoxygenase
Lipopolysaccharide
Liquidsolid chromatography
Low temperature inducer
Low volume irrigation
Manganese
Maturity index
Maleic hydrazide
modied olive medium
messenger RNA
Murashige and Skoog medium
Veliuron
Gene of cold tolerance, drought and salt stress
Newtons
1-naphthaleneacetic acid
naphthaleneacetamide or NAAmide
Nicotinamide adenine dinucleotide phosphate
Reduced NADP
Net assimilation rate
Near infrared
Nuclear magnetic resonance
N-1-naphthylphthalamic acid
Nitrate reductase
Optical density
Olive leaf extract
Wild olive
Olive medium
Olive mill waste water
Osmotic pressure
Photosynthetic active radiation
Programmed cell death
Protected designation of origin
A transcriptional repressor of HER 2 gene amplication
Phosphoenol pyruvate
Protected geographical indication
Photosynthetic rate
Photosynthetic photon ux
xiv
PRD
PWP
Q10
RAPD
RBCs
RD
RDI
RGR
RI
RNA
ROS
rRNA
RuDP
SA
SADH
SAM
SAR
SCAR
SDI
SLRV
SNP
SOD
SOS
sRNA
SSR
TDZ
TIBA
TLC
UV
Vmax
VPD
WHO
WPM
WUE
Zeatin
13C

leaf
p
S or
soil
Partial rootzone drying

Permanent wilting percentage
A temperature coefcient
Random amplied polymorphic DNA
Red blood cells
Responsive to dehydration
Regulated decit irrigation
Relative growth rate
Ripening index
Ribonucleic acid
Reactive oxygen species
Ribosomal RNA
Ribulose 1, 5-diphosphate
Salicylic acid
Succinic acid; 2, 2-dimethylhydrazide
S-adenosylmethionine
Sodium adsorption ratio
Sequence characterized amplied regions
Sustained decit irrigation
Strawberry Latent Rinspot Virus
Single nucleotide polymorphism
Superoxide dismutase
Salt overly sensitive genes, SOS1, SOS2, SOS3
Soluble RNA
Simple sequence repeats
Thidiazuron
2, 3, 5-triiodobenzoic acid
Thin layer chromatography
Ultraviolet absorption
Maximum rate of absorption
Vapour pressure decit
World Health Organization
Woody plant medium
Water use efciency
6-(4-hydroxy-3-methyl-2-butenylamino purine)
Carbon isotope discrimination
Total water potential
Predawn value
Turgor or pressure potential
Osmotic potential
Soil water potential
CHEMICAL SYMBOLS
B
C
Ca
CaCl2
CaCO3
Cl
Cu
Fe
H
H2PO4
H2SO4
HPO42
K
KCl
Mg
Mn
Mo
N
NH4+
O
P
S
SO42
Zn
Boron
Carbon
Calcium
Calcium chloride
Calcium carbonate
Chloride
Copper
Iron
Hydrogen
Dihydrogen phosphate anion
Sulfuric acid
Monohydrogen phosphate
Potassium
Potassium chloride
Magnesium
Manganese
Molybdenum
Nitrogen
Ammonium cation
Oxygen
Phosphorus
Sulfur
Sulfate anion
Zinc
xv
1
HISTORY OF OLIVE GROWING
The Olive Tree

I am the daughter of the sun
I am the olive tree
The blessed one.
K. Palamas
O olive tree, blessed be the earth that nourishes you and blessed be the water you
drink from the clouds and thrice blessed He who sent you for the poor mans lamp
and the saints candle-light.
Folk song from the Greek island of Crete
The olive tree is surely the richest gift of heaven.
Thomas Jefferson
The olive tree marks the route leading towards a higher stage of civilization. As
a native plant the olive tree was well known in the Mediterranean basin many
thousands of years ago. Hence, in the city of Kymi on the Greek island of Evia,
leaf fossils of Olea noti were found in carboniferous deposits dating back to the
Oligocene period.
Archaeological excavations have revealed the existence of olive leaf fossils
dating back to the Palaeolithic and Neolithic eras (37,000 BC) on Santorini
Island, Greece (Therios, 2005b). Furthermore, some ndings are reported for
the cretaceous strata of Provence, France and for North Africa. Olive pollen
was found in Greece, indicating its culture in this area. Hence, olive pollen
grains appear around the year 6000 BC (Epirus, western Greece) and around
3200 BC in Thessaly and eastern-central Greece.
Olive culture is very old. Fossilized remains of the olive trees ancestor were
found near Livorno in Italy, dating back 20 million years ago, although actual
cultivation probably did not occur in that area until the 5th century BC. Olive
trees are the oldest and one of the most important fruit trees (Standish, 1960).
Olive culture has been closely linked to the glory and decline of advanced
CAB International 2009. Olives (I. Therios)
Chapter 1
civilizations. The olive tree is the greatest gift of God, since it symbolizes peace
with its leaves and joy with its golden oil (see web site: History. Olives and
Mythology).
The olive is regarded as a symbol of euphoria, purity, victory and honour,
and olive shoots have been used to honour victory, peace and wisdom (Greek
olive oil). An olive shoot was returned by a dove to Noah on the ark, proving the
end of the great cataclysm. During the Olympic Games in Ancient Greece olive
wreaths were given to winners. Since olive trees offered food supplies,
agricultural communities were stable, evidenced by population growth, and
such societies existed for many years. Based on their productive olive orchards,
the Greek and Roman Empires developed into great economic forces;
destruction of olive orchards resulted in the decline of these once-great
establishments. Olive trees and olive oil have engaged the intellect, the senses
and the passions of the Greeks for as long as 4000 years; olive oil maintained a
sacred place in the Greek religion. Hence, in the Greek Orthodox religion olive
oil was used for both baptism and illumination of churches and houses; the
sacred lamp that was used in ancient Greece to light houses at night was
fuelled by olive oil. Furthermore, aged olive oil was used in sacred rituals of the
church at weddings. According to Herodotus (500 BC), the growing and export
of olive products were so sacred that olive culture was allowed to be performed
only by eunuchs and virgins (see web site: World Mythology Encyclopedia).
According to Greek mythology (Psilakis, 1996) Athena, the goddess of
wisdom, struck her spear into the earth and an olive tree appeared (see web site:
Stories of Athena). Athenas gift of the olive, which was useful for food, medicine,
perfume and fuel, was considered to be a more peaceful gift than Poseidons horse.
Athena planted the original olive tree in the Acropolis, Athens (see Fig. 1.1).
Thus, this location, where the rst olive tree grew, was named Athena
(Athens) to honour the goddess Athena. According to the mythology this
original olive tree still exists at the ancient sacred site and all Greek olive
orchards originated from leafy cuttings from the original tree. As stated by
Homer, the ancient olive tree in Athens was already 10,000 years old, and it
was forbidden to cut down any olive tree; the penalty for this was execution.
Athletes who competed in the ancient Olympic stadium (775 BC) were crowned
with a wreath made of olive shoots. The rst plantings and hand harvesting of
olive trees date back to 3500 BC, during the Minoan civilization (Evans, 1903;
Cadogan 1980) on the island of Crete (see Fig. 1.2).
Extensive plantings in Greece started in the year 700 BC. The signicant
Cretan civilization was active until 1450 BC, when it was devastated by an
earthquake after the explosion of the volcano of Santorini. In the ruins of
Knossos and Festos palaces large olive oil jars were found, and other discoveries
provide evidence of the commodities traded between cities and between
neighbouring countries (see Fig. 1.3).
The Minoan civilization (Hood, 1971) and its dominance in the islands
and the continental area of Greece could have been partly due to the existence
History of Olive Growing
Fig. 1.1. The goddess of wisdom, Athena, with the olive tree she planted in the
Acropolis, Athens, according to Greek mythology (Psilakis and Kastanas, 1999).
Fig. 1.2. Hand-harvesting of olive trees on Crete during the period of the Minoan
civilization (Psilakis and Kastanas, 1999).
Chapter 1
Fig. 1.3. Large earthen jars for olive oil storage in the Minoan palace at Knossos,
Crete (Psilakis and Kastanas, 1999).
of olive orchards. Archaeological studies supply circumstantial evidence for

the existence of olive trees in the main civilizations of the Aegean, i.e. Minoan,
Mycenaean (Nilsson, 1972) and Cycladic. Later, when the Minoan civilization
was in decline, the Phoenician civilization originated in the coastal area of
Lebanon. This civilization expanded beyond the Mediterranean and later
introduced olive trees to Carthage (North Africa) and to Spain (Terral, 2000).
Also, the Phoenicians spread the olive tree to the Mediterranean shores of
Africa and Southern Europe. Therefore, olive cultivation expanded to southern
Italy, Sicily, Corsica and Sardinia. Olives have been found in Egyptian tombs
dating from 2000 BC. Later, the Roman emperors protected olive cultivation by
law, and the countries of Morocco, Spain, Tunisia and Italy were the most
important sources of olive oil for the Roman Empire. The same protection for
olive trees was provided by the emperors of Byzantium, as well as by the Arabs.
Olives were rst cultivated in the Eastern Mediterranean region and
expanded westwards during the next millennium. Olive cultivation spread
from Crete to Syria, Palestine and Israel, to Cyprus, southern Turkey and
Egypt. With the expansion of the Greek colonies, olive culture reached
southern Italy and North Africa during the 8th century BC and subsequently
spread into southern France. In Israel, King Solomon and King David
expanded the practice of olive cultivation. Mohammed, the prophet of Islam,
suggested 1400 years ago to his believers that they apply olive oil to their
bodies. The use of olive oil is common in many religions and cultures. During
baptism in the Christian church, holy oil may be used as anointment. Like the
grape, the Christian missionaries brought the olive tree with them to
California, North America, not only for food but also for ceremonial use. Olive
oil was used to anoint the early kings of the Greeks and the Jews, and has also
been used to anoint the dead in many civilizations.
Evidence indicates that the olive was also cultivated in Syria and Palestine
from the 4th millennium BC. In Egypt the olive tree was also cultivated, as
indicated by a papyrus written around 1550 BC. Furthermore, mummies from
the 20th to the 25th dynasties discovered in Egypt were found to be wearing
olive wreaths.
More recent archaeological investigation has provided considerable evidence
concerning the existence of olives in ancient Israel. The olive tree was already
being cultivated by the Neolithic period, as evidenced by the recovery of olive
stones from excavations in Jericho. At most Palestinian sites, olive wood is not
found in large quantities until the Early Bronze Age, when extensive olive
cultivation coincided with the onset of urbanization (Goor, 1962).
Archaeological remains of olive wood provide more specic information.
The earliest wood remains yet found in ancient Israel belong to Olea europaea,
dating to 42,950 BC. The olive was one of the basic agricultural products in the
economy of Israel. In Egypt, excavation at the site of Karanis in the Fayoum
region has revealed that olive trees thrived in the area and that olive oil
production was carried out there on a large scale, at least in the 3rd century BC.
During the colonial period olives were dispersed by explorers and colonists.
In 1560 olive cuttings were carried to Peru by Spanish explorers. In the early
1700s, Jesuits established missions in Mexico and Baja California. Franciscans
founded their rst mission in California at San Diego in 1769. The olive tree
was introduced to California by the Franciscans as they marched north
establishing missions. A visitor to mission San Fernando in 1842 saw an olive
orchard in healthy productivity. Subsequently, over the intervening years, olive
trees have been planted in several waves and many of these older groves
(80150 years old) still exist in California, mostly in the northern part of the
state. However, in southern California population pressure has resulted in the
land being very expensive for olive growing.
Early olive plantings were established at a number of sites in New South
Wales, South Australia, Western Australia, Victoria and, to a lesser extent, in
other Australian states (Smyth, 2002). In the second half of the 20th century,
interest in olive growing coincided with the post-war migration of people from
European countries who were familiar with olive cultivation. Production of both
olive oil and table olives in Australia is increasing, especially of quality products.
Chapter 1
Today, the Australian olive industry is a signicant player in the domestic market
but not yet at an international level. Many changes during recent decades have
contributed to this agricultural success story. First, cultural changes have
occurred as a result of migration from Europe, due to the dietary changes
introduced by immigrants; this has resulted in a rise in olive oil consumption.
Other factors include the suitability of southern Australia for industrial-scale
olive cultivation, the rise in the Australian economy and the newly gained
horticultural experience (Kailis and Considine, 2002). It is expected that
Australia will soon produce about 1% of the worlds olive products.
The olive tree has been, for a very long time, a cultural element and a
common reference point and trademark for people leaving the Mediterranean
area (Simantirakis, 2003). We nd representation of the olive tree in ancient
coins (Greek and Roman), in the modern Greek drachma (20 cents coin) and in
the French franc (now superseded by the euro). Furthermore, decorations
depicting the olive are found on ancient Greek vessels, silver and golden items
and Egyptian sarcophagi. Famous painters like Renoir, Picasso, Dali and Van
Gogh included olive trees in their paintings. Also, major writers like Homer,
Pindarus, Virgilius (Virgil), Palamas, Seferis, Elytis, Lorca and others gloried
the olive tree in their work.
In our everyday routine the olive tree decorates towels, tablecloths and
gardens with Mediterranean landscapes. Hence, the olive tree is used to
determine the border of the Mediterranean zone: a zone that ends where olive
trees cannot grow and produce. Olive oil has been used both as currency and a
means of paying labourers or professionals; and olive trees were once used as a
dowry given to girls for their marriage.
Olive oil has many health benets when used in the culinary arena. It has
benecial effects on the digestive and cardiovascular systems due to its low
cholesterol content. Other actions include the prevention of wrinkles, dry skin
and acne, lowering of blood pressure, reduced muscular pain and strengthening
of the nails. The belief that olive oil conferred strength and youth has long been
widespread. In ancient Egypt, Greece and Rome it was infused with owers and
herbs to produce both medicines and cosmetics. Excavation in Mycenae, Greece
revealed a list of aromatics (mint, rose, juniper, sesame, etc.) added to olive oil in
the preparation of ointments.
The earlier ways of crushing olives included the use of a mortar and pestle,
and rolling a stone roller over them in a crushing basin. The second step in the
production of olive oil is pressing of the mass resulting from crushing. The rst
signicant advance in the production of olive oil was the use of a lever in a leverand-weights press, the use of which became common during the rst Iron Age.
Olive oil production became a mass production industry during Iron Age II.
WEB SITES
Greek olive oil, mythology and cultural inheritance: https://www.helleas.com/lire
History. Olives and mythology: https://www.itlv.com/encyclopedia/history/myths
Stories of Athena. 1. Greek mythology: https://www.theoi.com/Olympios/AthenaMyths
World Mythology Encyclopedia. Greek mythology: https://www.worldmythology.ws/
greek-mythology/birthof-hermes
2
THE OLIVE: ORIGIN AND
CLASSIFICATION
ORIGIN
The olive is native to the Mediterranean region, tropical and central Asia and
to various parts of Africa. The genus Olea includes at least 3035 species
belonging to the family Oleaceae and subfamily Oleoideae (x = 23). The
cultivated olive (Olea europaea L.) is an evergreen tree derived from tropical and
subtropical species. Fossils from olive species have been found in Italy, France
and in other countries.
The Mediterranean zone was, at one time, within the tropical zone, but
drought and glaciers during the Pleistocene period constituted a means of
natural selection for plants with the ability to avoid glaciers. The glaciers
probably reduced the initial olive tree population, and only plants with the
ability to survive at temperatures of between 5 and 12C survived.
Olive culture was known from the year 4800 BC in Cyprus. The longevity
of the olive tree may explain the great variability among its species. It is
believed that genotypes that were crossed under various climatic conditions
now constitute the species O. europaea. According to recent experimental
results, signicant genotypic variation exists among plants of the same
cultivar; genetic diversity was studied by Angiolillo et al. (1999), and
phylogenetic relations between Olea species by Ouazzani et al. (1993) and
Baldoni et al. (2002). Varieties tolerant to cold are present in the northern
areas of olive culture, although dry summers and mild winters are the most
appropriate climatic conditions for olive trees.
The olive tree originating from the Eastern Mediterranean is one of the
oldest cultures, belonging to the family Oleaceae with 30 genera, among
which there are certain decorative plants. Most of the olive groves belong to
the species O. europaea, with 2x = 46 chromosomes. The species O. europaea
includes many groups and more than 2600 cultivars, many of which may be
ecotypes.
Olea europaea does not seem to be a true species but one group of forms
derived from hybridism and mutation. The tropical and subtropical Afro-Asian
10
Chapter 2
species, such as O. chrysophilla and O. excelsa, probably participated in the

evolution of the culture.
Subspecies of olive are distributed in the Mediterranean countries and also
in West Africa, Tanzania, the Canary Islands, the Azores, South Africa, etc.
Olive trees have been introduced to the USA, Australia, South Africa and China
in more recent decades.
The native olive O. oleaster (Breton et al., 2006) and the cultivated olive
O. sativa are the main olive species in the Mediterranean. A classication of the
olive germplasm is presented by Ganino et al. (2006). The species O. oleaster
has thorny shoots, small oval to spherical leaves and small black fruits, with
low oil content. O. sativa has cylindrical shoots and big oval or elliptical fruits,
with high oil content. The genus O. oleaster is native in a small number of areas.
In some areas O. europaea is considered to be O. oleaster due to the juvenile stage
of plants created by grazing. These plants enter the reproductive stage after
appropriate treatments.
Countries with olive cultivation and the botanical species

Olive trees are cultivated in the following countries:
Europe: Spain, Italy, Portugal, France, Albania, Montenegro, Greece, Cyprus
Asia: Turkey, Syria, Lebanon, Jordan, Palestine, Iran, Iraq, Japan, China
Africa: Tunisia, Algeria, Morocco, Egypt, South Africa
America: USA, Mexico, Peru, Chile, Argentina, Uruguay
Oceania: Australia, New Zealand
Table 2.1 summarizes the current stage of olive cultivation in nonMediterranean areas.
The botanical species of olive grown throughout the various continents
are presented in Table 2.2.
Table 2.1. Olive cultivation in non-Mediterranean areas.
Continent
Asia
Oceania
America
Africa
Countries with
a tradition of
olive culture
Countries with
no tradition of
olive culture
Countries that
have started
planting olives
Countries
planting olives
experimentally
Iran
Iraq
USA
Argentina
Chile
Australia
Mexico
Peru
Uruguay
South Africa
Saudi Arabia
China
New Zealand
Brazil
India
Japan
Korea
Origin and Classication
11
Table 2.2. Botanical species of Olea, by continent.

Continent Species
Countries/areas of cultivation
Oceania
New Zealand
Australia
Australia, Java
America
Asia
Africa
Europe
O. apetala
O. paniculata
O. europaea
O. oribunda
O. americana
O. europaea
O. passiora
O. maritima
O. microcarpa
O. attenuata
O. dentata
O. lindley
O. salicifolia
O. dioica
O. cuspidata
O. compacta
O. roxburghiana
O. heyneata
O. glandulifera
O. acuminata
O. europaea
O. chrysophylla
O. laurifolia
O. verrucosa
O. verrucosa brachybotris
O. capensis
O. foveolata
O. concolor
O. exaesprata
O. humilis
O. obtusifolia
O. lancea
O. europaea
O. europaea
USA: Florida, Georgia, Carolina, Virginia

Chile, Peru, Mexico, Guadeloupe, Tahiti
Myanmar
India: Calcutta
India
India
Afghanistan, eastern India
India
Asia Minor
CLASSICAL TAXONOMY OF THE CULTIVATED OLIVE

Kingdom: Green Plants
Subkingdom: Tracheobionata
Superdivision: Spermatophyta
Division: Magnoliophyta
Class: Magnoliopsida
Subclass: Asteridae
12
Chapter 2
Order: Scrophulariales or Lamiales

Family: Oleaceae
Genus: Olea
Species: europaea
Other genera, except Olea (olive), within the family Oleaceae include the
following: Chionanthus (Snow ower), Forestiera, Forsythia (Golden bell),
Fraxinus (Ash), Jasminum, Ligustrum (Privet) and Syringa (Lilac). Some of the
better known plants belonging to the Oleaceae family are listed below
(common name(s) in parentheses).
Chionanthus retusus (Chinese fringe tree)
Chionanthus virginicus (Fringe tree, Snow ower)
Forestiera pubescens (New Mexico privet)
Forsythia intermedia (Border forsythia)
Forsythia mandschurica
Forsythia ovata (Korean forsythia)
Forsythia suspensa (Weeping forsythia)
Forsythia viridissima (Greenstem forsythia)
Fraxinus americana (White ash)
Fraxinus angustifolia (Narrow-leaved ash)
Fraxinus dipetala (Foothill ash)
Fraxinus excelsior (European ash)
Fraxinus latifolia (Oregon ash)
Fraxinus ornus (Flowering ash)
Fraxinus pensylvanica (Green ash)
Fraxinus quadrangulata (Blue ash)
Fraxinus velutina (Velvet ash)
Jasminum angulare (South African jasmine)
Jasminum oridum (Snowy jasmine)
Jasminum humile (Italian jasmine)
Jasminum le-ratii (Privet-leaved jasmine)
Jasminum mesnyi (Primrose jasmine)
Jasminum multiorum (Star jasmine)
Jasminum multipartitum (African jasmine)
Jasminum nitidum (Shining jasmine)
Jasminum nudiorum (Winter jasmine)
Jasminum ofcinale (Poets jasmine)
Jasminum parketi (Dwarf jasmine)
Jasminum polyanthum (Winter jasmine)
Jasminum sambac (Arabian jasmine)
Ligustrum amurense (Amur privet)
Ligustrum ibodium (Privet)
Ligustrum japonicum (Japanese privet)
Ligustrum lucidum (Glossy privet)
Ligustrum ovalifolium (California privet)
3
MORPHOLOGY AND TAXONOMY
OF THE OLIVE
The olive tree is a long-lived evergreen reaching 1000 years of age, or more.
Olive trees have a titanic resistance that renders them almost immortal (see
Fig. 3.1). In spite of cold winters and very hot and dry summers they continue
to grow, bearing fruit that nourishes and heals.
HABIT
The olive tree is an evergreen with greygreen leaves and small, white, fragrant
owers in the spring that produce a lot of pollen (Martin, 1994a). A mature
Fig. 3.1. A very old olive tree growing on the Greek island of Crete. Its age is
estimated to be over 1000 years, its trunk circumference is 15.6 m and its diameter
at 60 cm above the soil level is 4.5 m. This tree is still producing fruit.
13
14
Chapter 3
tree can reach a height of 2530 feet (810 m) and live for many hundreds
of years. An olive tree tends to grow densely, with thin branches. Cork cambium
is present and secondary thickening develops from a conventional cambial ring.
The cultivated olive belongs to the family of Oleaceae, genus Olea; the
scientic name is Olea europaea. In this family belong 30 genera and 180
species. The olive cultivars have 2x = 46 chromosomes. Excepting olive, in the
Oleaceae family various genera are classied accordingly:
Fleshy fruit
Dry fruit
Ligustrum
Chionanthus
Fraxinus
Syringa
Forsythia
According to De Candolle the genus Olea includes Gymnelaea and Euelaea,

along with the species listed in Table 3.1 (Therios, 2005b).
Olea europaea includes various sub-species (see below). For paternity
testing in olive progenies microsatellites are used (de la Rosa et al., 2002,
2004).
Olea europaea
euromediterranea
laperrini
cuspidata
sativa (cultivated olive)

oleaster (wild olive)
var. typical
var. cyrenaica
var. maireana
The olive tree is long-lived, with an average height of 1520 m. However,

under agricultural practices the height obtained following pruning is generally
45 m. The greatest proportion of roots grow at a soil depth of 6070 cm, the
thick roots growing in the top 20 cm. In dry areas the root system covers an
area seven to eight times greater than the leaf area (Fernndez et al., 1990).
When the soil is heavy the brous roots grow close to the soil surface. In sandy
soils, and generally in light-textured soils, the root system is extensive. In
Tunisia, a country characterized by a dry climate, the side-growth of roots is
12 m away from the trunk and the depth 6 m. With this deep root system the
olive tree absorbs water and nutrient elements from soils with very low rainfall.
The root system of olive trees (seedlings or asexual propagation) during the rst
34 years grows vertically, and subsequently this vertical root system is
replaced by a side-root system.
In the root system of olive trees, symbiosis with endotrophic mychorizzae is
very common (Mancuso and Rinaldelli, 1996); this phenomenon of symbiosis
Morphology and Taxonomy
15
Table 3.1. Various species of olive, by continent.

Europe
Asia
O. europaea O. europaea
O. microcarpa
O. maritima
O. passiora
O. heynana
O. attenuata
O. dentata
O. lindley
O. salicifolia
O. dioica
O. cuspidata
O. compacta
O. roxburghiana
O. glandulifera
O. acuminata
O. longifolia
Africa
America
Oceania
O. europaea
O. chrysophylla
O. laurifolia
O. verrucosa
O. branchybotris
O. capensis
O. faveolata
O. concolor
O. exaesprata
O. humilis
O. obtusifolia
O. lancea
O. americana
O. oribunda
O. apetala
O. paniculata
is greater in poor soils. Olive trees do not have a dominant taproot system, and
the root system is restricted to the rst 1 m of soil depth.
The tissue between the tree trunk and the root crown (xylopode) is
characterized by ovoid hypertrophies, containing sphaeroblasts (Therios,
2005b). About 30% of the total carbon xed by the plant is stored in this tissue,
from which the roots are derived. This form of root system may allow more
efcient water absorption after intermittent rainfall, in comparison with a deep
root system. One drought-avoiding response achieved with a deep root system
is to reach into moist soil. The ratio of the root size between irrigated and nonirrigated olive trees is 2.3:1. Irrigation reduces carbon transport to roots.
ANATOMY OF THE OLIVE TREE

Root system
The root system has the following functions:
Anchorage of the tree.

Water and nutrient absorption.
Synthesis of various organic materials.
Storage of nutrients.
The functions of the root system depend upon the rootstock, variety, soil
conditions and cultivation practices.
16
Chapter 3
Anchorage
The root is the most efcient anchorage mechanism for the tree when it is very
deep and branched. Such a root system is found most commonly when the
scion is vigourous and when the rootstock is a seedling and not originated from
rooted cuttings. A very deep and branched root system does not grow in
shallow soils or in soils having claypan, hardpan or a sand layer.
Absorption
Trees with a deep and well-branched root system are better adapted to water
and nutrient absorption. Water absorption is a function of soil properties and
the plant itself. The active absorption of nutrient elements requires utilization
of metabolic energy in the form of adenosine triphosphate (ATP).
Synthesis
In the root, gibberellic acids (GAs) and cytokinins are produced in the root tip.
Furthermore, in the root ethylene is synthesized, which at very low
concentration induces root growth and branching (Abeles, 1973). When
stress due to root damage is exerted a signicant amount of ethylene is
produced which, after its transport to the tree top, causes senescence and leaf
abscission. Another growth regulator, abscisic acid (ABA), is produced in the
root cap and, following its transport to leaves, is responsible for stomatal
closure.
Storage
In the root system carbon and nitrogen compounds are stored. Carbon is stored
in the form of starch and soluble carbohydrates, while nitrogen is stored as
amino acids and proteins. The accumulation of certain compounds at the end
of summer and early in autumn exerts a signicant inuence on both the
induction of owering buds and shoot growth the following spring. The storage
role of the root system and the other functions depend on the availability of
photosynthates. Every factor that reduces photosynthetic carbon xation leads
to a reduction in root growth. Under normal conditions, 50% of photosynthates are transported to the root, with 50% of these being utilized in
respiration and growth and the remainder being stored.
Root growth may be affected by the following factors:
PRUNING Severe pruning tends to reduce root growth during spring. This is
due to the fact that shoot growth precedes root growth and all photosynthates
are used for top and not for root growth.
Fungi, bacteria and nematodes reduce root growth. Adequate

soil moisture is a prerequisite for optimal root growth. Furthermore, excess soil
water reduces oxygen (O2) and root growth. Also, accumulation of salts and
especially those containing sodium (Na) and chlorine (Cl) in the soil or in
SOIL CONDITIONS
17
irrigation water reduces root growth. Concentrations considered to be toxic are

> 0.5% for Cl and > 0.2% for Na (d.w.). The root system could be a seedling or
clonal rootstock.
The use of dwarng rootstocks reduces the tree height and harvesting cost.
Hence, seedlings of the cv. Ascolano reduce the height of the cvs Mission,
Manzanillo and Sevillano. The rootstock O. oblonga reduces the height of
Manzanillo less than that of Mission, while it promotes the growth of Sevillano.
Also, the cv. Redding Picholine reduces the vigour of Manzanillo. Other genotypes tested as rootstocks, but without promising results, were O. ferruginea,
O. chrysophylla, Forestiera neomexicana, Fraxinus and Syringa vulgaris.
Trunk
The olive trunk is cylindrical, with an uneven surface, bearing a lot of
swellings. The wood is yellowish and darker towards the centre of the trunk.
The graceful, billowing appearance of the olive tree can be rather attractive. In
an all-green garden its greyish foliage serves as an interesting counterpoint.
The attractive gnarled branching pattern is also quite distinctive. The trees are
tenacious, easily sprouting back even when cut to the ground.
Main branches
The branches originate at a height of 1.2 m in the classical olive grove and at
2040 cm in the modern dense olive plantings. The number of branches is three
or more. The main branches give secondary and tertiary branching bearing the
leaves, owers and fruits. The small shoots are classied into four categories:
Vegetative shoots bearing only vegetative buds and producing new shoots
and leaves.
Fruit-bearing shoots bearing owering buds; their number is greater in the
low-vigour trees.
Mixed shoots bearing vegetative and owering buds concurrently; the
owers and fruits are borne at the base of the mixed shoots.
Water sprouts originating from the trunk, branches and the thick shoots;
these are very vigourous, grow vertically and they should be removed,
unless they are going to substitute for a low-vigour branch or stem.
Leaves
The leaves of olive trees are greygreen and are replaced at 23 year intervals
during the spring after new growth appears. The olives feather-shaped leaves
18
Chapter 3
grow opposite one another. Their skin is rich in tannins, giving the mature leaf
its greygreen appearance. Leaves have stomata on their lower surface only
(Fernndez et al., 1997). Stomata are nestled in peltate trichomes (see Fig. 3.2),
restricting water loss and protecting leaves against UV radiation
(Karabourniotis et al., 1992, 1995). The leaves are covered by a layer of wax and
cutin (cuticle). The deposition of cutin in the leaves takes place during leaf
growth and stops when leaf growth terminates. The weight of the cuticle on the
upper leaf surface is 1.4 mg/cm2; the thickness of the cuticle on the upper leaf
surface is 11.5 m, and on the lower one 4.5 m.
On both surfaces peltate trichomes exist and their concentration is
143/mm2 on the lower surface but only 18/mm2 on the upper. Stomates are
present (470/mm2) only on the lower surface (Martin, 1994a; Fernndez et al.,
1997). Leaf age affects stomatal conductance (Gucci et al., 1997b). Stomata
play a signicant role in sensing and driving environmental change
(Hetherlington and Woodward, 2003). The large number of trichomes
signicantly increases the efcient leaf surface, which is three times greater on
the lower surface in comparison with the upper. Both leaf surfaces are difcult to
hydrate and the contact angle of distilled water is 106 for the upper and 125
for the lower leaf surface. Table 3.2 summarizes these characteristics.
The leaves may have juvenile or adult characteristics. The juvenile and
adult phases can be differentiated by several morphological characteristics
such as thorniness, leaf size and shape, pigment accumulation, phyllotaxy and
ability to form adventitious roots (Hackett, 1985). Furthermore, in olive trees a
difference is recorded between juvenile and adult leaves concerning lipid and
protein composition (Garcia et al., 2000). Between the juvenile and mature
Fig. 3.2. Peltate trichomes on the lower surface of olive leaves (from Therios, 2005b).
19
Table 3.2. The main characteristics of olive leaves.

Characteristic
Upper surface
Lower surface
0
18
11.5
106.0
470
143
4.5
125.4
Stomates (n/mm2)
Trichomes (n/mm2)
Cuticle thickness (m)
Contact angle ()
stage there is a transition stage, which is followed by increasing internode

length and leaf blade size. Furthermore, during the transition phase changes
in composition take place. The changes (chemical, anatomical and
compositional) that occur during the transition stage affect the optical
properties of leaves. Hence, the electromagnetic spectrum could provide
information easily and quickly about the developmental phase of the olive
plant. The duration of the juvenile phase can be as long as 1520 years in olive
trees which creates problems for breeding and production of new olive
genotypes through crossing of certain parents. During the juvenile period an
olive plant cannot be induced to ower, but this is a drawback in breeding
programmes (Leon and Downey, 2006). The previous analysis indicates that
every effort should be exerted to shorten the length of the juvenile period
(Lavee et al., 1996). The optical properties of leaves are a useful criterion in
categorizing juvenile plants (Leon and Downey, 2006).
Buds
These are classed as either vegetative or owering. The former are small and
conical, while the latter are spherical and of greater size. Furthermore, latent
buds grow following severe pruning or after frost damage. A proportion of the
buds of the fruit-bearing shoot remains inactive.
Inorescences and owers

Inorescences are born in the axil of each leaf (see Fig. 3.3). Each inorescence
contains 1530 owers, depending on both the prevailing conditions and the
cultivar. Inorescences originate from buds of the current seasons growth.
These buds are induced to become owering ones after the winters chilling
effects. They then begin to grow, producing inorescences. The olive owers
are small, creamy white and hidden within the thick leaves. Each ower
consists of a four-segmented calyx, a tubular corolla with four lobes, two
stamens and an ovary with two carpels and a short style (Martin, 1994a). The
blossoms usually begin to appear in May. A wild seedling olive tree normally
20
Chapter 3
Fig. 3.3. Olive inorescence before and after blooming (cv. Chondrolia
Chalkidikis) (from Therios, 2005b).
begins to ower and produce fruit at the age of 8 years. The olive tree produces
both perfect and staminate owers. The perfect owers contain two stamens
and a well-developed ovary (green in colour), while staminate owers contain
an aborted ovary (very small in size) and normal stamens. The percentage of
perfect owers depends on many factors such as the cultivar, the number of
inorescences per plant, the soil moisture and the leaf nitrogen content during
the period of owering differentiation. The phenomenon of ower absorption
was mentioned by Theophrastus for olives in Italy as long ago as 350 BC.
Flower bud induction takes place in winter and 8 weeks before full-bloom
ower formation can be seen under a microscope. During the following 8
weeks ower development proceeds rapidly and owering occurs at the end of
May, or 12 weeks earlier in more southerly areas. The pistil of perfect owers
contains two ovules from which only one is fertilized, giving one seed. Figure
3.4 illustrates the duration of owering for a selection of cultivars over two
separate growing periods.
Fruit
The olive fruit is a drupe, spherical or elliptic in shape and consists of the
exocarp (skin), which contains stomata, the mesocarp (esh), which is the
edible portion of the fruit, and the endocarp (pit), including the seed. The fruit
of the olive tree is purplish black when completely ripe, but a few cultivars are
green when ripe and some olives develop the colour of coppery brown. The size
of the olive fruit is variable, even on the same tree, and depends on cultivar,
fruit load, soil fertility, available water and cultural practices (see Fig. 3.5).
Fig. 3.4. The duration of owering of 11 olive cultivars in two growing periods
(from Therios, 2005b).
Fig. 3.5. Olive tree with fruit setting (from Therios, 2005b).
21
22
Chapter 3
GROWTH, MORPHOLOGY AND PHYSIOLOGY OF THE

OLIVE TREE
Olive trees may attain a signicant size. However, the slow growth rate and the
height are important characteristics of this species olive trees require many
years for full fruit bearing. Shoot extension of olive trees occurs in spring and is
related to the rise in temperature. The growing tips are characterized by apical
dominance, and this inhibits the growth of axillary buds from the tip towards
the physiological base. Olive forms with both a physical spindle shape and
strong apical dominance give the greatest yields.
The vegetative buds start sprouting at the end of March, a little bit later
than the axillary owering buds. The spring vegetative wave is the most
important phase and lasts up to the middle of July. A second growth wave may
occur in SeptemberOctober after rain or irrigation. The length of annual
shoots is affected by fruit load, since fruits antagonize shoot growth. The
axillary buds induce or hinder growth from the middle of June to the middle
of October. Fruit removal or abortion of seed before hardening of the endocarp
(78 weeks after full bloom) increases the owering percentage. Injection of
GA3 (gibberellin) into the branches of trees not bearing fruits, between May
and November, inhibits owering the following year.
Between the middle of October and the middle of November a greater
quantity of RNA was recorded in the buds of trees that owered. These changes
lead to the conclusion that the induction of ower buds occurs up to the middle
of November. Subsequently, the buds enter into dormancy. The lack of growth
in the meristem of owering buds from the middle of November up to March
could be ascribed to two factors: (i) endodormancy; and (ii) ecodormancy.
Endodormancy leads to ecodormancy between the middle of January and
the end of February, depending on the cultivar. Sprouting of owering buds
triggers inorescence and owering bud growth. This is a continuous process
until owering. However, certain periods are critical for productivity. Hence,
the lack of water and nitrogen between bud sprouting and 6 weeks before
owering reduces the number of owers per inorescence and increases the
percentage of ovarian absorption. Furthermore, the embryo sac growth, which
lasts for a period of 20 days before owering up to full owering, may fail, due
to genetic factors. Flowering determines the initiation of the main period of
ower or fruit abscission and lasts for 6 weeks following full bloom.
Pollination and fertilization are followed by fruit set and growth. Of the
four ovules only one is fertilized, while the other three degenerate. Ovarian
growth triggers the abscission of the neighbouring, unfertilized, perfect owers
or the fruits with the smallest weight. The end of this period of abscission
coincides with hardening of the pit, and the rapid growth of the embryonic
fruit follows a double sigmoid curve, as a typical drupe. At the end of
the abscission period fruit size is 2025% of mature size. Fruit ripening starts
23
in the middle of October, completion time depending on the cultivar.

The maximum olive oil content is measured a little bit earlier than the
stage of full fruit ripening. The periods of ripening and harvesting of 25
Greek and 36 worldwide cultivated cultivars are given, respectively, in Figs 3.6
and 3.7.
Fig. 3.6. Ripening periods of 25 selected Greek olive cultivars. Ripening period: 1,
days 110 of the month; 2, days 1120; 3, days 2131.
24
Chapter 3
Fig. 3.7. Ripening periods of 36 selected worldwide olive cultivars.
4
STRUCTURE AND COMPOSITION
OF THE OLIVE FRUIT
STRUCTURE
The olive fruit can be separated into three distinct anatomical parts: epicarp
(skin), mesocarp (pulp or esh) and woody endocarp (stone), the last
containing the seed; these three fruit components follow distinctive growth
periods (Hartmann, 1949; Duran and Izquierdo, 1964; Farinelli et al., 2002).
Epicarp
This is the protective tissue that accounts for 1.03.0% of the drupe weight.
The skin is covered by a layer of wax, accounting for 4570% of the skin
weight. The skin is green during the early stages of development due to
chlorophyll, and later changes to straw yellow, pink, purple and black. This
colour change is due to varying concentrations of chlorophyll, carotenoids
and anthocyanins (Minguez-Mosquera and Garrido-Fernndez, 1989). The
epicarp plays a signicant role in minimizing mechanical damage and pest
attacks. Furthermore, the epicarp is impermeable to water, affecting the
processing of table olives.
Mesocarp
This is the most important part of the olive fruit, being the edible portion and
comprising 7080% of the whole fruit. The water content of the esh is
7075% of its weight and the oil content ranges between 14 and 15% in green
table olives and up to 30% in black mature olives. The table olives should have
low oil content. However, in certain types of black olives lipid biosynthesis
results in medium to high oil content (Donaire et al., 1984).
Olives contain oxalic, succinic, malic and citric acids and high levels of free
fatty acids. The sugars glucose, fructose, saccharose and mannitol 3.5 to
25
26
Chapter 4
6.0% of the esh in total are all present. The sugar content decreases with
maturation. Protein content ranges between 1.5 and 2.2% of the fruit by
weight. The pectic substances cement the cells and affect the texture of the
olive esh. These pectic substances during processing are hydrolysed by
pectinolytic enzymes and the fruit texture becomes softer.
Endocarp (stone)
The endocarp represents 1027% of the olive by weight, the seed comprising
24% of the weight. The seed contains 2227% oil and the shell 1%. Some
important characteristics of the endocarp include size, weight and ease of
separation (cling or free stone).
COMPOSITION
Table olives are characterized by the following parameters: (i) shape and fruit
size; (ii) the ratio of esh to pit; (iii) esh rmness; (iv) skin thickness; (v) stone
size and shape; and (vi) taste of the nal product. Varieties with large fruit size
are used for table olive processing. The time of harvesting for table olive
cultivars depends on the type of the nal product and it is a function of climatic
conditions. The determining factors include the following.
Colour
This depends on the intended use of the olive. Hence, for green olives the colour
will be straw-yellow green, with a deep black skin for black olives due to
anthocyanins, which could be evaluated in situ (Agati et al., 2005). The
amount of esh should not be lower than 70%. Furthermore, the esh should
be rm enough for the processing to be performed without loss of fruit quality.
The composition of the various parts of the olive fruit is presented in Tables 4.1,
4.2 and 4.3.
Table 4.1. Main composition of the olive fruit (from Marsilio, 2006).
Fruit
Pericarp
Pericarp/endocarp
Weight (%)
Epicarp
Epicarp/mesocarp
Pulp
Stone
Seed
7090
1027
13
Structure and Composition
27
Table 4.2. Chemical composition of the olive fruit pulp, by weight.

Component
Weight (%)
Moisture
Lipids (oil)
Reducing sugars
Non-reducing sugars
Crude protein (N 6.25)
Fibre
Phenolic compounds
Organic acids
Pectic substances
Minerals
Others
5075
1030
26
0.10.3
12
14
13
0.51.0
0.30.6
0.61.0
37
Table 4.3. Composition (percentage) of CHCl3EtOH-extractable classes of

compounds in the epicarp, mesocarp, shell and seed of cv. Coratina.
Compound
Epicarp
Mesocarp
Shell
Seed
Alkanes
Squalene
Alkyl esters
Methyl phenyl esters
Alcohols
Aldehydes
Free fatty acids
Pentacyclic triterpene alcohols
Pentacyclic triterpene acids
Free sterols
Steryl esters
Triacylglycerols
2
2
10
2
1
6
63
1
trace
0.1
trace
trace
5
trace
0.2
trace
trace
92
1.7
0.1
7
1.5
0.6
trace
1.1
78
trace
trace
0.3
0.2
8
0.4
4
trace
2
80
Alkanes
The major alkanes are found in both the epicarp and the woody part of the
shell. They are a mixture of C23C33 compounds; of those the odd chains C25,
C27 and C29 dominate. The dominant component in green olives is C29, while
in the black olive it is C27.
Alcohols, aldehydes and fatty acids

Alcohols are absent in the pulp, 0.10.2% in the stone and 10% in the skin.
The major chains are C22C28. Aldehydes are found in the skin in the even
28
Chapter 4
chains C26C32. Fatty acids consist of two groups, C16C18 and C22C28.
Alkyl esters are found in signicant amounts in the skin lipids and at trace
levels in the seed and pulp. Two groups are present, C40C44 and C46C56
chains. The dominant chains are C40 and C42 in the rst group and C52 and
C54 in the second one.
Triterpenoids
In the esh and seed are found cycloartenol and methylene cycloartenol.
Furthermore, pentacyclic triterpene acids are found in the epicarp, such as
oleanolic and maslinic acids. Free sterols and terpenes are present in signicant
amounts in the cuticular lipids, and the stony endocarp contains steryl esters.
Among the sterols the most important are -sitosterol, stigmasterol and
campesterol.
Phenols
Olive esh contains 114% (d.w.) phenolic compounds, depending on the
variety (Amiot et al., 1986). The phenolic compounds are important in many
aspects, such as protection of plants from bacteria, fungi and viruses (Hanbury,
1954) and fruit browning as maturation proceeds. Furthermore, phenolic
compounds play signicant roles in human nutrition (Bravo, 1999) and health
(Christakis et al., 1982; Manna et al., 1999). The bitter taste of raw olives is
due to phenols and, especially, oleuropein, which is water soluble (Gutirrez
et al., 1992). Other phenols include ligstroside, verbascoside, 4-hydroxytyrosol,
tyrosol and glucosides or aglycones, 3,4-dihydroxyphenyl glycol and avonoids
(Manna et al., 1999). The concentration of phenols is a function of fruit
maturity and can be as high as 14% (d.w.) in young fruits, though it may be
close to zero in black-type fruits.
The size of the fruit, which is a characteristic of the cultivar and irrigation
method employed (Costagli et al., 2003), is related to phenol concentration.
Hence, varieties with small-sized fruits have a high oleuropein content
compared with large-fruited varieties. As olive fruits mature, oleuropein
concentration is reduced (Amiot et al., 1989) while the levels of oleoside-11methylester and dimethyloleuropein start to increase, reaching maximum
levels when the fruit is black and mature. Therefore dimethyloleuropein is the
major component of black olives. Furthermore, as maturation procceds,
verbascoside concentration increases.
Another phenolic compound, ligustroside, is abundant in the early stage,
but levels decrease during fruit development. The oleuropein content depends
on the variety and its origin. Hence, the Greek and Italian cultivars contain
greater amounts of oleuropein in comparison with the Spanish and Portuguese.
29
Oleuropein
Oleuropein is present in all parts of the olive tree and especially in the leaves, at
concentrations of 6090 mg/g dry weight (Soler-Rivas et al., 2000). Olea
europaea contains, besides oleuropein, other phenolic glucosides such
as verbascoside (ester of caffeic acid and hydroxytyrosol), ligustroside,
dimethyloleuropein (acid derivative of oleuropein) and cornoside. Phenolic
composition differs between the various cultivars. Among the phenolics the
three most important are oleuropein, dimethyloleuropein and verbascoside.
Of all the olive varieties, the small-fruited ones contain high levels of
oleuropein and low amounts of verbascoside, while the opposite is true for the
large-fruited varieties. Other phenolics common in olive cultivars are tyrosol,
hydroxytyrosol and tyrosol glucoside. Furthermore, olive leaves and buds
contain avonoids such as quercetin, kaempferol and hesperitin. Also found in
the olive fruit are the avonol glycosides such as luteolin 7-glucoside, rutin and
anthocyanin (cyanidin, delphinidin).
Oleuropein belongs to the secoiridoids, found very commonly in
the Oleaceae (Damtoft et al., 1993). These compounds are produced from the
secondary metabolism of terpenes. Oleuropein is an ester of hydroxytyrosol.
Oleuropein biosynthesis in the olive commences during secondary
metabolism, via a branching of mevalonic acid. This produces oleosides, which
nally lead to formation of secoiridoids. The carbon skeleton is derived from
mevalonic acid. The precursors for oleuropein in olives are both epoxides of
secologanin and secoxyloganin.
BIOSYNTHESIS
Oleuropein is abundant in the young olive fruits and comprises

around 14% of dry matter. Also, ligustroside and cornoside are abundant.
While the fruits mature and their colour changes from green to black, the
emerging compounds of oleuropein are elenolic acid, glucoside and
dimethyloleuropein and glucosylated derivatives of oleuropein. At the green
mature stage and when the fruit turns black, dimethyloleuropein is the major
substance produced by the activity of esterases. The olive fruit accumulates
glucosylated derivatives of oleuropein. Another transformation of oleuropein
includes dihydroxytyrosol, which is found in leaves.
Other oleocides, which are reduced as oleuropein declines, include
ligustroside, while verbascoside levels are increased. Furthermore, tyrosol and
hydroxytyrosol are present in olive fruits during ripening.
DEGRADATION
OLEUROPEIN AS A PROTEIN DENATURANT AND AS A DEFENCE MECHANISM AGAINST
Oleuropein is a secoiridoid glycoside, which is common in the

family Oleaceae such as olives and Ligustrum obtusifolium. Oleuropein is
stable and is retained in a compartment separate from the activating enzymes.
When herbivores digest the leaves, enzymes present in the organelles activate
oleuropein and transform it into a strong protein denaturant, which promotes
HERBIVORES
30
Chapter 4
protein cross-linking and decreases lysine levels. These changes have adverse
effects on herbivores by decreasing the nutritive value of the dietary protein.
The enzyme -glucosidase in organelles converts oleuropein to a glutaraldehyde-like substance. Therefore, oleuropein activated by -glucosidase has
very strong protein-denaturing, protein-cross-linking and lysine-alkylating
activities, and L. obtusifolium has developed an effective defence mechanism,
with oleuropein as a protein cross-linker.
Antioxidant activity of olive oil
Among the phenolic compounds found in extra virgin oils, gallic, caffeic,
vanillic, p-coumaric, syringic, ferulic, homovanillic, hydroxybenzoic, protocatechuic acids, tyrosol and hydroxytyrosol are the most important. During
cooking under domestic conditions the oil is heated to temperatures of up to
190C. Polyphenols of extra virgin oil are stabilizers of -tocopherol during
heating (Andrikopoulos, 1989). Therefore, they contribute to the nutritional
value of cooked foods, and polyphenols prevent the decrease in antioxidant
activity in olive oil during heating.
Olea europaea extracts containing oleuropein and hydroxytyrosol were
found to be much more effective than vitamin E in their antioxidative activities.
These extracts owe their antioxidative properties both to their high oleuropein
content (19% w/w) and to a lower amount of avonoids (1.8% w/w). Tyrosol
showed neither antioxidant nor pro-oxidant activity (Lerutour and Guedon,
1992).
Phenolic biosynthesis
In olive plants the aromatic amino acids, phenylalanine and tyrosine, are
produced via the shikimate pathway. Furthermore, carbohydrates supply carbon
skeletons necessary for the biosynthesis of acetate, shikimic acid and aliphatic
amino acids. The initial reactants are phosphoenolpyruvate and erythrose-4
phosphate, which are produced via the non-oxidative glycolysis of glucose.
Phenylalanine is the precursor of most phenolic compounds. Phenylalanine
ammonia lyase (PAL) is the enzyme involved in phenolic biosynthesis.
Hydroxytyrosol in olive fruits
Hydroxytyrosol is a natural phenolic compound that is present in olive fruits,
table olives, olive oil pomace and waste water produced during processing of
olives. When olives were treated with NaOH, the major phenolic compounds
present were hydroxytyrosol, tyrosol, caffeic acid and p-coumaric acid.
Hydroxytyrosol glucoside is a polar substance and it is logical to expect its
presence in the waste water and not in the olive oil.
Phenolic compounds in various olive varieties
Phenolic compounds in olive fruits are a very important factor in the
evaluation of the quality of virgin olive oil, since they are responsible for its
31
antioxidation stability. Oleuropein is the main substance giving bitterness to

olives. The phenolic content of virgin olive oil is affected by the variety, location,
degree of ripeness and the type of machinery used, e.g. three-phase or twophase decanter (Di Giovacchino et al., 1994; Servili et al., 2006).
Phenolic content of waste water and its antioxidant activities
Olive oil is extracted mechanically by pressure and by a three- or two-phase
decanter. The three-phase centrifugation needs 06 m3 H2O/t of olives and is
the most extensively used method for virgin olive oil production. The twophase decanter separates virgin olive oil by recycling the waste water produced
from processed olives. The two-phase extract has the highest antiradical
activity, which is twice the value obtained by the three-phase extract. The
compound exerting most antioxidant activity is hydroxytyrosol (Galli and
Visioli, 1999).
5
HECTAREAGE, NUMBER OF TREES
AND PRODUCTION OF OLIVE OIL
AND TABLE OLIVES
OVERVIEW
Most olive trees, on a worldwide basis, are cultivated in the Mediterranean
region. More than 75% of the global olive production occurs in Europe, which
cultivates 500 million olive trees. Europe is followed by Asia (13%), Africa (8%)
and America (3%). Spain is the leader in olive culture, followed by Italy and
Greece.
The hectareage and number of olive trees worldwide are given in Table 5.1,
the average annual total world production of olives in Fig. 5.1, the global olive
oil production in Table 5.2, the olive oil production in the Mediterranean in
Table 5.3, the EU production of olive oil in Table 5.4 and the world production
of olive oil (percentage of total) in various countries in Fig. 5.2.
In recent decades olive oil production has had periods of growth followed
by stagnation. At the beginning of the 1980s world production was about
Fig. 5.1. The average annual total world production of olives during the period
19982001 (15,090,620 t) (from FAOSTAT, 2003).
33
34
Chapter 5
Table 5.1. Hectareage and number of olive trees worldwide (2005). Data
elaborated using the International Olive Oil Council (IOOC) statistics.
Country
Spain
Italy
Greece
Cyprus
Rest of Europe
Total, Europe
Argentina
USA
Mexico
Rest of America
Total, Americas
Turkey
Syria/Lebanon
Palestine
Rest of Asia
Total, Asia
Tunisia
Morocco
Algeria
Rest of Africa
Total, Africa
Others areas
Total, World
Hectareage (ha 1000)
Trees (n 1000)
2,340
2,250
670
12
1,203
6,475
70
44
15
11
140
723
168
66
2
959
1,240
222
166
117
1,742
681
10,000
200,000
185,000
133,000
2,450
62,150
7,000
582,600
4,500
1,540
1,570
14,610
72,000
24,160
12,130
240
108,530
52,000
22,000
16,000
4,550
94,000
12,550
800,000
Fig. 5.2. World production of olive oil (percentage of total, 19992000; total
2,033,500 t) (from IOOC).
Hectareage, Number of Trees and Production
35
Table 5.2. World production of olive oil (1000 t), 19982000 (from IOOC).
1998/2000
Country
EU1
Spain
France
Greece
Italy
Portugal
Tunisia
Turkey
Morocco
Syria
Algeria
Other
countries
Total
1EU,
1999/2000
Production
Consumption
Export
Production
Consumption
Export
1,698.5
789.2
3.4
473.0
397.0
36.0
215.0
170.0
65.0
115.0
39.5
70.6
1,660.0
500.0
78.8
245.0
705.0
67.0
49.0
97.0
55.0
88.0
35.0
392.2
235.0
75.0
1.0
6.0
140.0
12.5
175.0
60.0
20.0
4.0
0.0
14.6
1,563.0
550.0
3.0
426.0
620.0
40.0
200.0
70.0
40.0
80.0
25.0
55.5
1,679.0
500.0
80.0
245.0
710.0
68.0
65.0
60.0
50.0
85.0
29.0
391.9
257.0
80.0
1.5
7.0
150.0
17.5
120.0
30.0
10.0
5.0
0.0
9.4
2,373.6
2,385.2
508.6
2,033.5
2,359.9
431.4
European Union; incorporates several of the countries in this table.
Table 5.3. Production of olive oil in the

Mediterranean area (percentage of total,
by country) (from IOOC).
Country
Production
Spain
Italy
Greece
Portugal
Tunisia
Turkey
33
23
17
8
8
5
1.8 million t, 40% above the value recorded in the mid-1960s. After a relatively
stable period production again increased in the second half of the 1990s, to
reach 2.5 million t. Average world production for the most recent years is about
2.7 million t.
The EU is the dominant producer on the olive oil market. Until 1981 its
425,000 t accounted for only one-third of world production. EU production
after the accession of Greece, Spain and Portugal rose sharply, averaging about
80% of world production.
During the 1990s a rapid rise in production in the EU occurred due to the
doubling of Spains production and an increase of 1618% from Greece and
Italy. The next planned expansion of the EU will have limited impact on EU olive
36
Chapter 5
Table 5.4. Olive oil production in the European Union (1000 t, 19922003) (from
IOOC).
Year
1992/1993
1993/1994
1994/1995
1995/1996
Average
1996/1997
1997/1998
1998/1999
1999/2000
Average
2000/2001
2001/2002
2002/2003
Average
Spain
Italy
Greece
Portugal
France
Total
623.1
550.9
538.8
337.6
512.6
947.3
1077.0
791.9
669.1
871.3
973.7
1411.4
865.0
1083.4
435.0
520.0
448.0
620.0
505.75
370.0
620.0
403.5
735.0
532.1
509.0
656.7
590.0
585.2
310
254.0
350.0
400.0
328.5
390.0
375.0
473.0
420.0
414.5
430.0
358.3
375.0
387.8
22.0
32.1
32.2
43.7
32.5
44.8
42.0
35.1
50.2
43.0
24.6
33.7
29.0
29.1
1.6
2.3
2.0
2.3
2.1
2.5
2.7
3.4
4.1
3.3
3.2
3.6
4.7
3.8
1391.7
1359.3
1371.0
1403.6
1381.4
1754.6
2116.7
1706.9
1878.4
1864.2
1940.5
2463.7
1863.7
2089.3
oil production, since only three of the new members are producers (6000 t for
Cyprus, 400 t for Slovenia and 150 t for Malta, annually). These three
members represent 0.4% of the national guaranteed quantities of the other
member states. The global production of table olives is presented in Table 5.5
and Fig. 5.3.
Olive oil accounts for only about 3% of the global market of edible oil.
World consumption of olive oil has been progressing steadily (see Table 5.6),
Table 5.5. Global production of table
olives (1000 t, 19992000) (from IOOC).
Country
EU1
Spain
France
Greece
Italy
Portugal
Turkey
Morocco
Syria
Argentina
Egypt
Other countries
Total
1EU,
European Union
Production
563.5
380.0
2.0
92.0
80.0
9.5
100.0
85.0
75.0
103.5
40.0
142.5
1109.5
37
Fig. 5.3. World production of table olives (percentage of total, 19992000; total
1,149,500 t) (from IOOC).
Table 5.6. World consumption of olive oil (1000 t, 19952003) (from IOOC).
Year
1995/96
1996/97
1997/98
1998/99
1999/00
2000/01
2001/02
2002/03
EU
USA
Japan
Australia
Canada
Other
EU (%)
1402
1687
1841
1824
1844
1918
1994
2028
105
144
152
159
174
212
221
225
17
26
34
29
28
30
32
33
17
22
18
24
24
31
28
29
14
19
18
19
20
25
24
26
374
473
485
501
480
497
461
490
72.7
71.9
72.2
71.4
71.8
70.7
72.2
71.6
the EU being the worlds largest consumer. Other minor consumers in the
Mediterranean basin are Syria (100,000 t), Turkey (70,000 t), Morocco
(50,000 t) and Tunisia (40,000 t). Of the non-European countries the USA is
the worlds second biggest consumer of olive oil. Appreciable rises were also
recorded in Australia, Japan, Canada and Brazil. The EU is the worlds leading
consumer of olive oil, averaging 1.8 million t, representing 71.6% of world
consumption in 2002/2003. The main consumers in the EU are Spain, Italy
and Greece, together accounting for more than 85% of the unions total
consumption.
Through the 1990s, olive oil consumption showed an increase of 3.3%
in the EU as a whole, but growth was smaller in Greece, Spain and Italy
38
Chapter 5
(2.22.6%). The consumption rate rose much faster in Portugal (9.7%) and
France (10.8%) in the 1990s. The per capita increase in consumption of olive
oil in the EU over that decade is presented in Table 5.7, while EU exports for the
period 19922003 are shown in Table 5.8.
Greek exports consist mainly of extra virgin olive oil (73%), while those of
Italy, Spain and Portugal represent 45, 44 and 21%, respectively. Most of the
EUs exports are directed to the USA, Australia, Japan, Canada and Brazil.
Other major exporters are Turkey (to Canada, the USA, Australia), Tunisia (to
the USA) and Argentina (to Brazil). The average prices of exported olive oil are
given in Table 5.9.
The ratio of the prices of olive oil to other edible oils is 45:1. The difference
in quantities of olive oil produced from year to year results in major variation in
the prices paid to producers; consumer prices tend to be more stable. In Italy
and Greece producer prices for olive oil followed a downward trend in the
1990s. The producer price (/t) for extra virgin olive oil in Italy fell from an
average of 3015.8 to 2318.7 towards the end of the decade. For Greece this
gure dropped from 2708.6 to 1905.2 and in Spain from 2775.9 to 1826.6.
Table 5.7. Apparent consumption of olive oil in the EU (kg per capita) (from IOOC).
Year
1990
2000
Increase (%)
Greece
Spain
Italy
Portugal
France
Other
Whole EU
20.2
25.0
2.2
10.1
12.6
2.2
9.5
12.3
2.6
2.7
6.9
9.7
0.5
1.4
10.8
0.1
0.5
16.1
3.3
4.6
3.3
Table 5.8. EU olive oil exports ( 1000 t, 19922003) (from IOOC).

Year
Spain
Italy
Greece
Portugal
France
Other
Total
1992/93
1993/94
1994/95
1995/96
Average
1996/97
1997/98
1998/99
1999/00
Average
2000/01
2001/02
2002/03
Average
51.6
54.6
54.0
48.8
52.3
66.7
76.2
63.6
87.7
73.6
88.3
112.5
115.0
105.3
90.8
104.8
105.8
90.5
98.0
129.5
123.5
125.3
182.7
140.3
173.0
182.9
200.0
185.3
10.3
9.2
5.5
11.0
9.0
5.2
8.0
5.4
8.2
6.7
10.0
10.0
15.0
11.7
7.5
10.5
13.1
11.8
10.7
17.0
17.4
12.4
17.5
16.1
17.3
16.2
16.0
16.5
0.9
1.1
1.0
1.1
1.0
1.1
1.1
1.0
1.4
1.2
1.3
1.0
1.1
1.1
0.3
2.5
3.1
1.6
1.9
0.7
1.0
0.9
1.0
0.9
1.1
1.7
0.8
1.2
161.4
182.7
182.5
164.8
172.9
220.2
227.2
208.5
298.5
238.6
291.0
324.3
347.9
321.1
39
Table 5.9. Average annual prices (/t) of exported extra virgin and lampante1 olive
oil, 19922002 (from IOOC).
Year
1992/93
1993/94
1994/95
1995/96
Average
1996/97
1997/98
1998/99
Average
1999/00
2000/01
2001/02
Average
Spain
Italy
Greece
Extra virgin Lampante
2244.5
2424.9
2770.4
3663.9
2775.9
2467.0
1812.2
2325.2
2201.5
1919.8
1712.9
1847.2
1826.6
2151.3
2322.1
2622.4
3473.0
2642.2
2045.4
1564.2
2164.7
1924.8
1782.5
1598.0
1733.3
1704.6
2586.2
2772.8
2855.3
3849.0
3015.8
3564.6
2507.7
2591.1
2887.8
2268.8
2250.0
2437.2
2318.7
2128.1
2287.8
2538.1
3385.8
2585.0
2170.5
1545.1
2122.7
1946.1
1734.7
1547.6
1708.7
1663.7
2265.6
2419.0
2557.8
3592.0
2708.6
2765.1
2011.9
2221.2
2332.7
1841.4
1752.2
2122.1
1905.2
1799.4
1960.6
2118.0
2864.8
2185.7
1986.4
1364.3
1781.8
1710.8
1514.1
1391.4
1482.2
1462.6
1Lampante
oil is of a grade not intended for human consumption; the word derives
from the ancient use of olive oil as a fuel for lamps.
TABLE OLIVES
World production in 2003 was about 1.6 million t (see Table 5.10). There is a
variety of table olives (whole, pitted, sliced, stuffed) and, as observed in the case
of olive oil, the production of table olives uctuates from year to year, due to
both weather conditions and the alternate bearing habit of olive trees. When
the price of olives for the production of table olives is low, part of the yield is used
for oil production. The EU is the largest table olive producer (40%), others being
Turkey (13%), the USA (10%), Morocco (8%), Syria (7%) and Egypt (4%).
Table 5.10. Global production of table olives (1000 t, 19952003) (from IOOC).
Year
EU
Turkey
Syria
USA
Morocco
Egypt
Total
1995/96
1996/97
1997/98
1998/99
1999/00
2000/01
2001/02
2002/03
369
370
486
500
621
576
765
614
120
185
124
178
150
224
75
165
75
90
60
85
93
142
80
170
66
144
91
78
129
60
120
81
85
100
85
95
80
80
90
80
60
25
50
41
85
70
135
300
947
1063
1094
1201
1351
1343
1464
1647
40
Chapter 5
The average production from the EU in the period 20002003 was

651,000 t (see Table 5.11): Spain, 478,000 t (73.4%), Greece, 100,000 t
(15.3%), Italy, 62,000 t (9.5%), Portugal 10,000 t (1.5%) and France, 2000 t
(0.3%).
The average world consumption of olives in 2003 was about 1.6 million t
(see Table 5.12). The EU is the greatest consumer (33%), followed by the USA
(13%), Egypt (10%), Turkey (9%), Syria (7%) and Brazil (3%).
EU consumption (see Table 5.13) comprises 39.8% for Spain, 29% for Italy,
7.8% for France, 5.4% for Greece, 2.6% for Portugal, 6.6% for Germany and
2.6% for the UK.
Table 5.11. Table olive production from the EU (1000 t, 19922003) (from IOOC).
Year
1992/93
1993/94
1994/95
1995/96
Average
1996/97
1997/98
1998/99
1999/00
Average
2000/01
2001/02
2002/03
Average
Spain
Italy
Greece
Portugal
France
Total
224
205
236
203
217
244
310
360
431
336
416
575
442
478
70
77
60
86
73
55
80
45
75
64
65
60
60
62
60
60
60
70
63
60
85
85
100
83
85
115
100
100
17
16
10
9
13
9
9
9
13
10
9
12
10
10
2
2
2
1
2
2
2
2
2
2
2
2
2
2
372
360
368
369
367
370
486
500
621
494
576
764
614
651
Table 5.12. Global consumption of table olives (1000 t, 19952003) (from IOOC).
Year
EU
USA
Syria
Brazil
Egypt
Turkey
Total
1995/96
1996/97
1997/98
1998/99
1999/00
2000/01
2001/02
2002/03
352
319
341
392
430
454
525
520
149
173
179
177
184
185
205
205
71
80
65
70
91
110
74
111
47
48
50
48
51
45
51
51
48
29
33
44
77
57
75
160
129
132
127
149
130
125
100
135
1043
1066
1075
1186
1250
1297
1380
1575
41
Table 5.13. EU consumption of table olives (1000 t, 19922003) (from IOOC).

Year
19921996
average/year
19962000
average/year
20002003
average/year
Spain
Italy
Greece
Portugal
France
Other
Total
120
125
27
17
28
33
348
135
115
22
12
33
55
372
199
145
27
13
39
76
500
WEB SITE
International Olive Oil Council (IOOC): https://www.internationaloliveoil.org
6
ROOTSTOCKS
The planting material for olive orchards is originated from either budded
seedlings or leafy cuttings. Budded plants are preferable, since the rootstock
affords plants a better adaptability to stress conditions. Olea europaea seedlings
and especially those of wild olive are often used with budding or grafting.
However, composite plants show signicant variation concerning tree vigour
and productivity.
Among olives more suitable as rootstocks, with no variation in tree vigour,
are included the rooted cuttings of a vigourous variety. Under orchard
conditions the olive tree has to adjust to certain problems, such as cold stress
(Charlet, 1965), water stress, salinity and, in moist soils, Verticillium wilt and
other pathogen problems (European and Mediterranean Plant Protection
Organization, 2006). One such rootstock is O. oblonga (Hartmann et al., 1971).
Other Olea species do not provide satisfactory rootstocks for olive trees. Genera
and species tested as rootstocks for olives include Phyllirea, Ligustrum, Syringa,
Chionanthus, Forsythia, Fraxinus forestiera, O. verrucosa and O. chrysophylla.
Their compatibility with olives is very limited. In addition to poor growth
characteristics, incompatibility is most evident through unsatisfactory union.
Therefore, a defective union is produced, leading to a defective union structure.
In addition, rooted leafy olive cuttings from low-vigour cultivars can be used as
dwarng rootstocks.
The root system of rooted olive cuttings differs from that of olive seedlings,
at least during the rst years of life, as rooted cuttings have all their roots
originating at the base of the cutting and growing at the same soil level. In
contrast, in plants grafted to seedlings the roots originate along a 40 cm axis
and produce an angle greater than 90. Therefore, such roots grow more
deeply into the soil. Such differences result in roots more tolerant to water
stress and cold.
43
44
Chapter 6
ROLES OF ROOTSTOCKS
The rootstocks of olives affect the size of the tree, its mineral nutrition
(Chatzissavvidis and Therios, 2003), the fruit size and shape and the season of
ripening.
Tree size
New systems of olive planting, such as super-high density, have started to
expand all over the world. Therefore, the reduction of tree vigour is very
important in olive culture. Today dwarfed rootstocks, as in the case of apples,
are not available. Very dwarfed rootstocks are not the most appropriate.
Certain rootstockscion combinations achieve the dwarng effect (Troncoso et
al., 1990; Lavee and Schachtel, 1999). These combinations are exemplied by
the following:
Mission, Sevillano (Hartmann and Whisler, 1970) and Manzanillo

grafted on to Ascolano seedlings.
Self-rooted Sevillano gives relatively small trees appropriate for highdensity planting.
Manzanillo grafted on to Oblonga.
Self-rooted Mission and Manzanillo give more vigourous trees than
grafted-on rootstocks.
Fruit size, shape, ripening time and mineral content of

olive fruits
No consistent trend between scion and olive fruit ripening season was
recorded. Fruit shape could be affected or not by the kind of rootstock (Connell
and Catlin, 1994). For example, the length:width ratio of the fruits of the
Mission cultivar is greater in self-rooted plants in comparison with grafted
ones on various rootstocks. Furthermore, olive fruit size and/or productivity
are affected positively or negatively by grafting, depending on the cultivar
(Caballero and del Rio, 1990). Moreover, the mineral content of olive leaves is a
function of the rootstock used.
Salt tolerance
Ion toxicity is one way in which salinity can adversely affect olives. Olive trees
are considered moderately tolerant of salinity, though certain cultivars are
Rootstocks
45
more tolerant of salinity than others. However, their responses may differ
depending on the rootstock (Connell and Catlin, 1994). Unfortunately, the
relative responses of olive rootstocks are not known.
DESCRIPTION OF SELECTED OLIVE ROOTSTOCKS

Olea oblonga
This is a seedling rst found in California in 1940. This rootstock is Verticillium
dahliae Kleb. resistant but not immune to Verticillium. Oblonga is easily
propagated under mist or fog using small leafy cuttings. Grafting of sensitive
cultivars to V. dahliae resistant rootstocks reduces their susceptibility (Porras et
al., 2003). The Oblonga rootstock has a signicant dwarng inuence on
Manzanillo, less dwarng on Mission and an invigorating effect on
Sevillano. Sevillano scions grafted on to this rootstock produced large and
heavy-yielding olive trees (Connell and Catlin, 1994).
Allegra
This is also a rootstock resistant to Verticillium wilt. When Allegra is used as
rootstock the Verticillium is transmitted though the rootstock to the susceptible
scion.
Wild olive (Oleaster)

Oleaster olives differ from the cultivated clones by the presence of spinescent
juvenile shoots, smaller fruits with less mesocarp, lower oil content and
furthermore by a long juvenile stage, long-lasting in some seedlings. The
genetic variations of Oleaster can be analysed by using RAPD markers, RAPDs
and allozyme polymorphism.
Olive oil and table olive cultivars

Mission
Mission seedling rootstocks are variable, and this variation may account for
variation in commercial olive orchards grafted on to Mission seedling
rootstock. Cultivars grafted on to Mission rootstock include Manzanillo and
Sevillano.
46
Chapter 6
Nevadillo
This is an oil-producing cultivar and is recommended as rootstock for
Sevillano, Mission and Manzanillo cultivars.
Redding Picholine
Redding Picholine is a large-fruited variety and is used as a rootstock for
Sevillano and Mission.
Megaritiki, Chondrolia Chalkidikis, Amphissis, Koroneiki, Agiou
Orous and Matolia
In one experiment own-rooted cvs Megaritiki, Chondrolia Chalkidikis,
Amphissis, Kalamon, Koroneiki, Agiou Orous and wild olives as well as
scion rootstock combinations were grown in a greenhouse (Chatzissavvidis,
2002). The lowest boron (B) concentration in leaves and roots was found in
Kalamon and wild olives, respectively. Megaritiki had higher leaf B concentration when grafted on to Megaritiki or Chondrolia Chalkidikis compared
with own-rooted plants. The same cultivar as own-rooted plants had higher
root B concentration than a rootstock of the other tested cultivars. Budding
reduced stem potash (P) concentration of Megaritiki and Chondrolia
Chalkidikis grafted on to Chondrolia Chalkidikis and Amphissis, respectively.
Chondrolia Chalkidikis, as a rootstock, increased stem potassium (K) in
Megaritiki. Similarly, Amphissis and Megaritiki, as rootstocks of Amphissis,
reduced stem K concentration. Budding of Chondrolia Chalkidikis on to
Chondrolia Chalkidikis or Megaritiki, and Amphissis to Megaritiki, both
reduced stem calcium (Ca) concentration.
In Table 6.1 the concentration of B in four rootstocks (O. oblonga,
Chondrolia Chalkidikis, Matolia and wild olive) at six B concentrations in the
nutrient solution is presented and indicates that the maximum increase of B in
leaves was recorded at a treatment rate of 20 mg B/l in the cultivar Matolia
(897%) (Chatzissavvidis, 2002).
The accumulation of B increased very rapidly in all the treatments and in
a relatively short time. In the following Table (6.2) is presented the correlation
between B concentration in the nutrient solution and mineral concentration in
the leaves of the four rootstocks (O. oblonga, Chondrolia Chalkidikis, Matolia
and wild olive).
CLASSIFICATION OF ROOTSTOCK VIGOUR

The various degrees of vigour of olive plants exhibited by common rootstocks
are as follows:
Rootstocks inducing increased growth: Acebuche, Moriscade, Badajor,

Lechin de Sevilla, Real Sevillana and Cornezuelo.
Rootstocks
47
Table 6.1. Boron (B) concentration at 30, 60 and 90 days after initiation of the
experiment and percentage change in B concentration at 90 days in comparison
with that at 30 days (from Chatzissavvidis, 2002).
Variety
Olea oblonga
Chondrolia
Chalkidikis
Wild olive
Matolia
Days
Treatment
(B, mg/l)
30
60
90
Change (%,
3090 days)
0.27
1.00
2.50
5.00
10.00
20.00
0.27
1.00
2.50
5.00
10.00
20.00
0.27
1.00
2.50
5.00
10.00
20.00
0.27
2.50
20.00
26
20
24
30
50
83
21
22
25
31
37
55
23
27
25
36
54
111
38
36
23
28
26
34
53
97
147
27
34
29
45
54
153
35
39
38
62
110
114
27
49
56
21
27
49
89
189
239
22
32
37
87
143
126
15
37
118
259
255
24
59
233
17
+34
+104
+193
+279
+187
+6
+49
+47
+180
+283
+129
34
+38
+52
+224
+381
+130
35
+63
+897
In wild olives (2.50 mg B/l) the per cent change represents a period of 60 days.
Rootstocks inducing normal growth: Gordal, Blanqueta, Tempranilla

de la Sierra, Changlot, Manzanillo de Jan.
Rootstocks inducing little growth: Redondilla de Logrono, Picual,
Hojiblanca and Habichuelero.
Listed below are other methods of reducing growth.
Use of growth regulators; the GA3 inhibitor uniconazole had too drastic an
effect on tree size. The increased yield due to uniconazole treatment is due
to increased fruit setting; similar growth inhibition was achieved with
paclobutrazol.
Selection of a suitable cultivar for high-density systems.
The best cultivars for super-high-density planting were Askal, Arbequina,

Arbosana and Koroneiki.
48
Chapter 6
Table 6.2. The correlation between boron (B) concentration in the nutrient solution
and mineral concentrations in the leaves of Olea oblonga, Chondrolia Chalkidikis,
Matolia and wild olive, used as rootstocks (Chatzissavvidis, 2002).
Olea oblonga
B
N
P
K
Na
Ca
Mg
Fe
Mn
Zn
Chondrolia Chalkidikis
Matolia
Wild olive
Base
Top
Base
Top
Base
Top
Base
+ top
0.934**
0.723**
NS
0.756**
NS
NS
NS
0.877**
NS
0.679**
0.993**
0.698**
NS
0.737**
NS
0.707**
NS
0.635**
NS
NS
0.570**
0.845**
0.440*
0.832**
NS
0.537**
NS
0.530**
NS
NS
0.586**
0.802**
0.563**
0.598**
NS
0.422*
NS
0.549**
NS
NS
0.843**
NS
NS
0.585*
NS
0.877**
NS
NS
NS
NS
0.707**
NS
NS
0.849**
0.563*
0.808**
NS
0.808**
NS
0.777**
0.960**
0.761**
NS
0.532**
NS
0.841**
NS
0.593**
0.456*
NS
N, nitrogen; P, potash; K, potassium; Na, sodium; Ca, calcium; Mg, magnesium; Fe, iron; Mn,
manganese; Zn, zinc.
Statistically signicant differences for P 0.05 (*), 0.01 (**) and < 0.001 (***).
7
MAJOR TRENDS IN OLIVE
FARMING SYSTEMS
The total area under olive cultivation within the EU has evidenced signicant
uctuation within its various member states and areas since the 1970s.
Throughout the 1970s, in all the EU countries, a decrease in the number of
olive groves was recorded. This could be ascribed to EU programmes aimed at
digging out old trees. During the following decade olive cultivation began to
expand in certain Mediterranean countries such as Greece and Spain and, to a
lesser extent, Italy and Portugal.
Signicant changes in cultivation techniques have occurred since the
1970s, involving such as the use of chemical fertilizers, herbicides and
insecticides and modern cultivation and irrigation systems. The expansion of
new plantation systems and the abandonment of the old, traditional ones have
produced signicant environmental consequences. We shall now examine
what changes and progress in olive culture have been at work in the three main
EU olive-producing countries, i.e. Spain, Italy and Greece.
SPAIN
During the mid-1960s olive groves in Spain covered 2.4 million ha, while
during the following decade many old plantations were cleared out and
replaced by arable crops. In 1986, Spain joined the EU and, with the support of
the Common Agricultural Policy (CAP), olive cultivation increased both in
terms of production and the total area of olive groves, partly through the
introduction of new plantations, these new olive plantations replacing arable
crops and grassland areas. In the year 1995 alone 67,000 ha of olives were
planted in Spain and, from 1990 to 1998, about 150,000 ha of new olive
groves were developed. In other words, the average rate of planting from 1990
was around 20,000 ha/year and the total olive area in Spain is now over 2.4
million ha. Plantations developed after 1998, according to the EU support
policy, cannot receive EU aid.
49
50
Chapter 7
It was in the 1980s that olive growing became more intensied, with
biological production and chemical pest control being practised in many areas
with the exception of marginal ones. In Spain the use of fertilizers is
increasing, and likewise irrigation in new plantations. Another new technique
introduced to Spain is mechanical harvesting for both new, dense planting
systems and traditional plantations. Biological olive oil production is
increasing, but still represents only a small proportion of the total olive grove
area. Furthermore, integrated pest management systems have been developed
in olive groves in Spain but they represent only a small percentage of the total
area.
ITALY
The latest data from EU surveys indicate that the total olive-growing area is
1.4 million ha, which is greater by about 400,000 ha when compared with the
area in existence in the 1990s. There are signicant differences in planting
trends in various regions. Hence, in some areas old olive trees were grubbed out
and some new and more efcient cultivars planted. Furthermore, many
traditional orchards were transformed into modern, densely planted orchards.
A new trend in Italy is the planting of biological olive groves, and the area of
those in 1997 was 15,200 ha, which still represented a small proportion.
However, their area is now increasing due to nancial support from the EU
(Regulation 2078/92).
GREECE
The area of olive orchards has increased signicantly since the late 1970s. This
is due to the planting of new olive orchards in the high-density system (67 m
between trees and 67 m between rows). Furthermore, olive oil and table olive
production have both increased over the same period. This enhanced
productivity is due to various factors: (i) mechanization; (ii) land levelling; and
(iii) irrigation, wherever there is available water. Other factors responsible
include: (i) the high level of EU support; (ii) high prices for olive oil: (iii)
improvements in olive cultivation and mechanization; (iv) establishment of
new, intensive plantations; (v) biological olive oil production; and (vi) efcient
water use by means of drip irrigation (Metzidakis and Koubouris, 2006).
On many small Greek islands, and also in marginal, semi-mountainous
areas, olive production is generally not intensive due to difcult soil/climatic
conditions (lack of irrigation water, shallow soil, soil inclination and difculties
with mechanization, use of labour in touristic areas, etc.). Under such
circumstances olive production yields only a low income.
8
CLIMATIC AND SOIL CONDITIONS
CLIMATIC CONDITIONS: TEMPERATURE REQUIREMENTS

The olive tree is cultivated under various climatic and soil conditions and its
cultivation is feasible throughout the entire temperate and subtropical zone,
i.e. between 30 and 45 (Bongi and Palliotti, 1995). The olive tree could also
grow under tropical conditions without producing fruits, unless it is cultivated
either: (i) at adequate elevation in order to full its needs of low temperatures;
(ii) if the cultivars have low chilling requirements; or (iii) by supplemental
pollination (Denney et al., 1985; Ayerza and Sibbett, 2001; Ayerza and Coates,
2004). The greatest olive tree accumulation is recorded in the Mediterranean
countries with mild winters and hot, dry summers.
The areas of olive culture have a mean annual temperature 1520C, with
a minimum of 4C and a maximum of 40C. The minimum temperature
should not drop below 7C, otherwise damage to trees occurs. However, this
limit is only an estimation, since the tolerance of olive trees depends on various
factors such as the duration of very low temperatures, atmospheric humidity,
cultivar, etc. Damage also varies from one tree to another. If the drop in
temperature is gradual the olive tree can stand temperatures down to 12C
(Bongi and Palliotti, 1995). The sensitivity of olive trees to frost reduces the
extension of olive cultivation northwards; in the marginal northern limit of
olive cultivation olive culture is conned to coastal areas.
Due to the late blooming of olives, spring frosts are not a determining
factor in olive culture. Olive trees require low temperatures for owering bud
differentiation and the requirement of vernalization explains why the tree does
not produce in tropical regions. Some varieties such as Sevillano and
Ascolano require about 2000 h below 7C. Not only low but also high
temperatures play a signicant role, especially during the summer, since these
instigate fruit drop.
Areas of high altitude are not appropriate for olive culture, due to frost
danger and to the shorter vegetative period. Commonly, olive trees are not
planted at altitudes greater than 800 m in Mediterranean countries. High
51
52
Chapter 8
relative humidity is responsible for disease problems. Hail damages olive trees
by damaging the fruits and by increasing the danger of Bacterium savastanoi
infection.
Although olive trees are tolerant to wind, wind-affected areas should not
be used for olive culture. Cold, moist and hot winds during spring reduce ower
fertilization and fruit growth. Furthermore, hot winds during the summer
instigate fruit drop.
Concerning soils, olive trees can grow well even in poor, dry, calcareous
and gravelly soils. However, the best soils for annual bearing are the deep,
sandy-loam soils adequately supplied with nitrogen (N), P, K and water.
Among the fruit trees olives are considered to be very tolerant to water stress.
Root density is positively related to tolerance to water stress and water
absorption. Hence, in olives the ratio of roots:leaves is 74:1, while in Actinidia
chinensis (kiwi fruit) it is 910:1. The root length of olive plants having the
same age as kiwi fruit plants is four times greater in comparison with A.
chinensis.
The available soil volume/tree is signicant. Clay soils that have high
moisture content and immobilize K and P are not suitable for olives. Also, soils
with a hardpan close to the soil surface are not appropriate for olives. Another
factor is that the sodium chloride (NaCl) content of soils should be less than
1g/l.
Olive trees grow and produce in soils with both medium acid and medium
alkaline pH; however, pH values greater than 8.5 reduce growth signicantly.
Furthermore, olives grow with no toxicity problems in soils with a relatively
high boron content (Chatzissavvidis, 2002).
Heat stress and optimum temperature range for olive trees

Adequate knowledge of the optimal thermal range is essential in quantifying
thermal stress and in assessing the efciency of the tolerance or resistance
mechanisms in plants (Mahan et al., 1995). High temperature stress has
negative effects on olive plants and, as a consequence, olive yields are reduced
(Denney et al., 1985; Mancuso and Azzarello, 2002). This imposes limitations
on the expansion of olive culture in heat-stressed environments. The
determination of the optimal thermal range is useful in order to predict the
ability of the plant to tolerate heat stress; when we say heat stress tolerance we
mean the ability of the plant to maintain its productivity when exposed to
thermal stress.
Temperatures below and above the optimal thermal range determine the
low and high points of temperature stress. The heat load of the olive plant and
the energy exchange within the olive tree canopy are a function of many
factors such as leaf size, shape and leaf orientation. The architecture and the
pruning system of olive trees can modify the temperature of the olive leaf
Climatic and Soil Conditions
53
canopy, thus affecting water consumption and heat stress. Other important
factors for increasing the tolerance of olive plants include the following:
Root:leaf ratio.
Leaf orientation, size, shape and characteristics of the surface. The existence
of pubescence in leaves reduces heat stress problems.
Leaf thickness, stomata size and distribution.
Optimal root system, which absorbs sufcient water in order to maintain
olive canopy temperature.
Molecular techniques can increase the thermal stress tolerance by

incorporation of genes giving heat tolerance. The incorporation of enzymes
with different heat tolerances into the olive plant will widen the range of
optimum temperatures.
Thermal requirements of the olive

Temperature is the important climatic factor that geographically limits olive
culture. Olive trees do not survive temperatures less than 12C. However,
olives require a period of low temperatures (07C) for owering bud
differentiation. Therefore, temperature limits olive cultivation to the range of
3045 latitude. Olive oil quality is improved in areas with cold winters, and
this explains why 1015% of olive oil production is located in such areas
(Bongi and Palliotti, 1995). The olive yield/m2 of leaves at 34.8 latitude is
50% greater in comparison with that at 45 latitude, due to both growth
decrease during winter and to a high degree of photoinhibition. The total
number of low-temperature hours should be at least 10 weeks with
temperatures <12.2C for ower bud differentiation (Hartmann, 1953;
Hartmann and Porlingis, 1957). The particular cultivar of the olive tree is the
determining factor in chilling requirements.
Constant temperatures of <7C or >15C can inhibit owering bud
induction, and winter temperatures of >20C for 23 weeks inhibit sprouting
of owering buds. The hormone balance of olive trees controls chilling
requirements; therefore, a late harvesting period could increase the chilling
requirements (Lavee and Harshemesh, 1990). The differentiation of owers
needs higher temperatures in comparison with those required for owering
bud induction. Furthermore, very high temperatures in spring may inhibit
ower development. Another factor is that high temperatures in spring reduce
the duration of owering and inhibit pollen tube growth (Galn et al., 2005). In
contrast, low spring temperatures lengthen the period of owering by up to
3 weeks and can also postpone owering or inhibit cross-pollination.
With regard to the germination of olive stones, the optimum temperature is
10C for 1 month before sowing (Voyiatzis and Porlingis, 1987). When the
temperature during sowing is 25C or higher it reverses the effect of chilling for
54
Chapter 8
a period of up to 5 weeks. The optimum temperature for photosynthesis depends

on the olive variety. Furthermore, temperature affects the accumulation of
various metabolites in the different parts of olive plants. For example, starch and
mannitol accumulation in the leaves is reduced during summer (Drossopoulos
and Niavis, 1988). During winter, starch concentration is reduced in the leaves,
while that of soluble sugars is increased. Also, the transport of mannitol from
bark to wood is reduced with low temperatures. Furthermore, low soil
temperatures induce a water decit (Pavel and Fereres, 1998).
The southern limit of olive cultivation is determined by winter temperatures,
which should be <14C for 2 weeks for owering bud induction. From the known
cultivars, Koroneiki, Arpa and Rubra have the lowest chilling requirements
(Hartmann and Porlingis, 1957) and can be used for expanding the southern
limit of olive cultivation. High temperatures reduce olive productivity, since these
destroy protein structure. On average, leaves and shoots are injured at
temperatures of around 48 and 50C, respectively (Mancuso and Azzarello,
2002). When temperatures reach >35C photosynthesis is inhibited, and the leaf
cuticle is irreversibly damaged at temperatures >55C. High temperatures are
associated with a high vapour pressure decit (VPD) between leaves and the
surrounding air. The same applies to fruit, where high temperatures may cause
fruit drop.
Low temperature stress

When winter temperatures drop below 5C, the acclimation and hardening
processes of the olive plants begin (Burke et al., 1976; Levitt, 1980; Alberdi
and Corcuera, 1991; Ruiz et al., 2006). With low temperatures sucrose
accumulates (Guy et al., 1992), starch is redistributed and is accumulated in
organs protected from frost, such as roots. Furthermore, changes in biochemical
parameters of olive leaves occur during the vegetative season, correlated with
frost resistance (Bartolozzi et al., 1999). Temperatures < 12C damage the leaf
canopy, shoots and branches. Buds and inorescences are also very sensitive to
spring frosts, and temperatures <10C during owering reduce the percentage
of pollination. When water freezes ice is produced initially in the leaf apoplast,
which has 100 times less density than the symplast. Before harvesting, frosts
cause shrinkage of olive fruits, wood and cambium browning, splitting of shoots
and necrosis of the whole tree. The matric potential (m) forces of the cell wall
inhibit ice formation in the apoplast at temperatures of down to 10C (Bongi
and Palliotti, 1995). When an ice crystal is formed in the apoplast, the cell
maintains its permeability to water and a signicant difference in water
potential () causes water loss from the symplast (Palta and Weiss, 1993). A
high degree of supercooling is observed in olives, which is a mechanism for
avoidance of ice formation (Fiorino and Mancuso, 2000). Leaves in which ice
has formed damp downwards following melting of the ice due to difculty in
55
reversion of plasmolysis. Furthermore, due to low winter temperatures olive

fruit will shrivel (see Fig. 8.1), shoots dry out (see Fig. 8.2), bark splits (see
Fig. 8.3) and wood and cambium become brown in colour (see Fig. 8.4).
Fig. 8.1. Olive fruit shrivelling due to low winter temperatures prior to harvesting.
Fig. 8.2. Shoots of cv. Koroneiki drying due to winter frosts.
56
Chapter 8
Fig. 8.3. Bark splitting on an olive tree after a severe frost.
Fig. 8.4. Wood and cambium browning on olive trees after severe winter frosts.
57
Low temperature sensing in olive trees and the role of calcium

The capacity of a species to survive at freezing temperatures is a function of the
cultivar and its ability to acclimatize to cold (Alberdi and Corcuera, 1991; Palta
and Weiss, 1993). Cold acclimation is followed by accumulation of cryoprotective
substances, which inuence membrane structure and anatomy. The leaves
perceive the cold acclimation stimulus and also produce the necessary chemicals
for cold acclimation (Weiser, 1970). Cold acclimation requires expression of
specic genes (Palya, 1993). However, in olives genetic cold tolerance has not
been studied in depth and only a few publications exist (DAngeli et al., 2003;
DAngeli and Altamura, 2007). Nevertheless, based on both experience and
macroscopic appearance, olive varieties can be classied as cold tolerant, cold
semi-tolerant or cold sensitive (Roselli et al., 1989).
Olive is an evergreen tree with low tolerance to frost, limiting its cultivation in cold regions, where the slow post-maturation process improves oil
quality (Palliotti and Bongi, 1996). Therefore, nding a way for tree survival in
the colder climate is of great signicance. However, a transgenic approach for
improving cold resistance in olives has not yet been carried out (DAngeli and
Altamura, 2007).
Calcium plays a very important role in freezing tolerance. Hence, in many
fruit trees exogenous application of Ca2+, in the form of sprays or fertilizers,
increases fruit and leaf Ca levels and thereby increases cold tolerance (Raese,
1987). From many experiments with olives it was found that young leaves are
more sensitive to cold than are older leaves, which have a greater Ca2+
concentration. The results of DAngeli et al. (2003) indicate that the nonacclimated olive leaf protoplasts counter a rapid fall in temperature by
increasing their Ca2+ concentration. This includes an inux of Ca2+ via the
plasmalemma and an efux of the Ca2+ from the organelles. A decrease of
0.075C/s is adequate to create an increase in Ca2+. An increase in Ca2+ is
genotype dependent and is also essential for the development of cold tolerance.
Therefore, Ca is involved as a signalling agent in cold resistance of olives, and
Ca2+ signalling could be an early marker for selection of cultivars for cold
resistance.
In order for olive trees to become cold tolerant the process of cold acclimation
is necessary, in which osmotin is involved. Osmotin is a pathogenesis-related
protein having cryoprotective functions. Plant cold acclimation involves the
acquisition of freezing tolerance by exposure to non-freezing low temperatures.
This process requires accumulation of cryoprotectants and anatomical changes
in specic tissues enabling the plant to become tolerant to extracellular freezing.
Like other species not displaying dormancy, the olive tree needs several weeks to
exhibit cold damage symptoms at the level of plant anatomy. Such symptoms
include necrosis of cambium, tylosis, suberization and cell wall lignication.
During adaptation to cold stress an increase in calcium concentration occurs. In
Olea europaea osmotin, which is a pathogenesis-related protein, is positively
58
Chapter 8
involved in the induction of programmed cell death (PCD). This protein is

produced during the cold acclimation period of the tree and involves more than
one of the processes leading to complete acclimation. Osmotin is also involved in
arresting cold-induced Ca signalling. The effect of long exposure to cold is
presented in Fig. 8.5 (DAngeli and Altamura, 2007).
Assessment of frost tolerance in the olive: methods

Various methods are used in order to assess frost tolerance in olive varieties
(Fiorino and Mancuso, 2000). These include the following: (i) visual
observations; (ii) electrical resistance changes during exposure to low
Fig. 8.5. The role of osmotin during cold acclimation in the olive. The stabilization
of F-actin and Ca2+ and the induction of programmed cell death (PCD) are
necessary for complete acclimation (from DAngeli and Altamura, 2007).
59
temperature; (iii) stomatal size and density; (iv) electrolytic conductivity; (v)
differential thermal analysis; and (vi) tissue staining.
Visual observations
This is the traditional method of screening chilling and freezing tolerance.
However, this method is not objective and is inuenced by various factors
including wind, relative humidity, and nutritional and water stress of the
plants. With this method scientists record their observations only of the leaf
canopy and not of the root system. At 12C trees are damaged, i.e. leaves die,
leaf abscission occurs and twigs dry up.
Chlorophyll uorescence quenching analysis
This method does not have adequate sensitivity and is too labour intensive.
Electrical resistance
With this method we measure electrical resistance changes within different
plant organs (Mancuso and Rinaldelli, 1996) and, based on the electrical
resistance changes, we determine the critical and freezing temperatures
(Mancuso, 2000). Both critical and freezing temperatures are lower for the
more chill-tolerant genotypes, and the absolute critical temperatures are lower
for the chill-tolerant varieties (8.8C for Ascolana) and higher for the chillsensitive ones (13.6C for Coratina). The sensitivity to chilling of various
organs in the olive follows the order: roots > leaves > shoots > vegetative buds.
Nevertheless, individual components do not represent the cold hardiness
of the whole plant. Therefore, for an estimation of frost damage it is better to
use the entire tree instead of merely parts of the tree.
Measurement of the changes in electrical resistance of olive tissues at low
temperatures consists of a simple, easy, accurate and non-destructive method
to screen those olive varieties having cold tolerance. The absolute critical
temperatures (Mancuso, 2000) for the cultivars Ascolano, Leccino,
Frantoio and Coratina are 8.8, 10.6, 12.6 and 13.6C, respectively.
Stomatal size and density
Stomatal density is used as a criterion for selection of cold-tolerant varieties
(Roselli et al., 1989; Roselli and Venora, 1990). From a study of stomatal
density in some Greek olive varieties we can classify them as follows:
Some varieties considered tolerant to cold had smaller stomatal density in

comparison with sensitive ones (see Fig. 8.6 and 8.7).
Varieties with the greatest stomatal density are: Pierias (551 stom/mm2),
Megaritiki (548 stom/mm2) and Maronias (547 stom/mm2).
The varieties with the smallest stomatal density are: Mavrelia Serron (441
stom/mm2), Koroneiki (441 stom/mm2) and Galatistas (355 stom/mm2).
60
Chapter 8
Fig. 8.6. Linear regression between stomatal density and cold tolerance of ten olive
cultivars. Cold tolerance: 1 = hardy, 7 = very susceptible (from Roselli and Venora,
1990).
Fig. 8.7. Parabolic correlation between stomatal perimeter and cold tolerance of
olive trees. Cold tolerance: 1 = hardy, 7 = very susceptible (from Roselli and
Venora, 1990).
In comparison with deciduous fruit trees, olive trees have greater stomatal
density. Figure 8.6 shows the linear correlation between stomatal density and
cold tolerance of ten varieties, while Fig. 8.7 shows the parabolic relationship
between stomatal perimeter and cold tolerance.
Electrolytic conductivity
Electrolytic conductivity is a sensitive method and is utilized to determine
differences between olive cultivars with regard to frost resistance (Murray et al.,
61
1989). Leaves were exposed in a cold room for 4 h, with a controlled drop in
temperature of 7C/h. Determination of the cold tolerance of olive leaves is
achieved by measuring the conductivity of leaf disk extract. Thirty leaf disks,
1 cm in diameter, were placed in a plastic bag and 10 ml of distilled water at
25C added for 6 h. Another 20 disks were incubated for 1 week at room
temperature in order to determine the degree of cold damage. Recording of
damage was conducted with image analysis, being measured by both image
analysis and measurement of electrical conductivity of the leaf disk extract.
The relative release of electrolytes (REL) is calculated from the equation:
REL = [(L1/L2) 100]
where L1 is the rst measurement of electrical conductivity. This represents the
liberation of ions caused by cold damage but also includes ion release under
normal conditions; L2 is the second measurement of electrical conductivity and
represents the conductivity in the dead tissue due to cold. This represents the
total quantity of the electrolyte in the leaf disk. Cold tolerance can be expressed
as LT50 (temperature at which 50% of tissues are damaged).
Differential thermal analysis (DTA)
Olive trees exposed to low temperatures survive either by avoidance of ice
formation in their tissues (supercooling) (Bongi and Palliotti, 1995) or by
increasing their frost hardening and resistance. Supercooling is generally
studied by the differential thermal analysis (DTA) technique (Fiorino and
Mancuso, 2000).
Olive plants survive winter temperatures by deep supercooling of tissue
solutions to temperatures close to those of homogenous nucleation of the
aqueous solution (40C). When this fraction of the solution freezes, an
exotherm can be observed by DTA (Fiorino and Mancuso, 2000). In order to
conduct DTA, excised olive tissues are placed on one side of a thermopile plate
and a dried sample of tissue on the other as a control in order to measure the
differential temperature changes between the two samples during the process
of freezing. The samples are placed in a freezing cabinet and cooled down to
30C at 5C/h. During this process we measure the signals from the
thermopiles.
Tissue staining
Five cross-sections of two to three cells width were cut with a razor blade from
the middle of leaves. Cutting was performed under demineralized water and
maintained under various temperatures for 20 min in a 0.02% solution of
neutral red [3-amino-7-dimethylamino-2-methyl-phenazin (HCl)]. The
samples were stored at 5C for 24 h in order to absorb the dye. For each
different temperature 100 cells per section were examined under a light
microscope (200 magnication). Only living cells absorb the dye and exhibit
62
Chapter 8
intense red coloration. The percentage dye absorption of the control is

measured at temperatures from 0 to 20C (Fiorino and Mancuso, 2000).
Dye absorption was reduced depending on cultivar, by: 1432% at 10C;
2164% at 14C; and 82100% at 16C. Finally, at 20C all tissues were
dead (see Table 8.1).
Comparison of the methods electrolytic conductivity (REL), visual
observation and DTA gave comparable results. However, signicant differences
in cold tolerance exist between olive varieties.
Frost tolerance of olive varieties and methods of protecting

olive trees against frost
Frost affects plant cell membranes and these become less permeable and even
porous, allowing leakage of electrolytes. Certain cultivation techniques can
reduce the danger of cold damage; such techniques include: (i) reduction of
irrigation and N fertilization; (ii) maintenance of the soil in a bare condition;
and (iii) late pruning and use of cold-hardy varieties, since cold tolerance is a
genetic characteristic. The high K:Ca ratio in the plant increases tolerance to
cold and drought. Spraying olive trees with copper (Cu)-containing chemicals
(such as Kocide) hardens olive trees and protects them from frost. Spraying was
conducted during winter. The Cu-containing sprays protect against both frost
and bacterial infection (Antognozzi and Catalano, 1985; Antognozzi et al.,
1990c, 1994; Martin et al., 1993; La Porta et al., 1994; Bartolozzi and
Fontanazza, 1995, 1999; Mahan et al., 1995; Barranco et al., 2005).
Foliar application of meuidine (Palliotti and Bongi, 1996), a plant growth
regulator, at 1000 ppm concentration to cultivars sensitive to frosts, induced a
hardening effect and increased freezing resistance by reducing the leakage of
K+, Na+ and Ca2+ through the various biological membranes (Palliotti and
Bongi, 1996). The maintenance of bare soil increases its temperature and a
bare soil is warmer during a frost in comparison with that covered by
vegetation. Furthermore, stony soils have higher temperature and waterlogged
soils are colder than well-drained soils.
Table 8.1. Neutral red absorption at temperatures below freezing for four
olive cultivars.
Neutral red absorption (percentage of control at 0C)
Variety
0C
5C
10C
12C
14C
16C
20C
Ascolano
Leccino
Frantoio
Coratina
100
100
100
100
100
100
100
100
86
76
73
68
82
66
53
51
79
51
35
36
18
12
0
0
0
0
0
0
63
Olive varieties resistant to cold include the following (Barranco et al., 2005).
The frost-hardiest cultivars are Cornicabra, Arbequina and Picual; other
frost-hardy cultivars include Mission, Leccino, Carolea, Chemlali, Moraiolo
and Picholine. Conversely, the most frost-susceptible variety is Empeltre. The
cultivar Hojiblanca is generally considered cold hardy (Barranco et al., 2000)
and is classed together with Frantoio in the intermediate cold-resistance group.
From the Greek varieties those considered most resistant include the
following: Galatistas, Arvanitolia Serron and Mavrelia Messinias. Kalamon
has medium tolerance and Koroneiki, Amphissis and Chondrolia Chalkidikis
are sensitive to cold.
SOIL CONDITIONS
Important characteristics of soils
Soil is a complex system consisting of varying proportions of four components,
i.e. mineral particles, non-living organic matter, the soil solution and air
occupying the pore space. Furthermore, soil gives shelter to many living
organisms such as bacteria, fungi, algae, protozoa, insects and small animals.
Mineral particles comprise the main part of most soils and the most stable
component. These are derived by weathering of mother rocks or deposition by
water or wind. Organic matter constitutes less than 2% of soil volume, with the
exception of organic soils. The term organic matter includes the various
organic residues such as roots, litter and decomposition products. The solid
matrix forms a pore space which constitutes 3060% of soil volume. The pore
space at eld level is lled with soil solution at 4060%, while the remainder is
lled with air. In saturated soils the entire porosity is lled with water, whilst in
dry soils most of the pore space is lled with air. Excepting organic matter,
water and air, soil provides shelter for microorganisms that help in the decay of
organic matter and release of mineral nutrients. Small animals, such as
earthworms and insects, drill tunnels in the soil, which improve inltration
and water movement.
The properties of a soil depend on soil texture, i.e. size and distribution of
mineral particles, their arrangement and the type of clay minerals. The clay of
soils consists of four minerals, i.e. kaolinite, montmorillonite, vermiculite and
illite. Kaolinite has a 1:1 proportion of silica to aluminum and its crystals have
the least tendency to shrink or swell upon hydration changes. Montmorillonite
and illite have a 2:1 silica:alumina ratio and soils containing these minerals
swell and shrink markedly upon hydration or drought, and crack signicantly
upon drying. The signicant characteristic of clay minerals is that, during
replacement of silicon or aluminum with other cations, they develop a negative
charge, which determines their efciency in holding cations or their cation
exchange capacity (CEC). Furthermore, the organic matter content contributes
64
Chapter 8
to CEC. The CEC of clay minerals and organic matter is as follows: organic matter
(100300 meq/100 g dry soil) illite, 3, kaolinite, 10, montmorillonite, 100
and vermiculite, 160.
Soil texture
The relative contents of sand, silt and clay in the soil determine soil texture,
including sand, loam, silt, clay and various intermediate classes such as sandy
loam, silt loam or clay loam. The diameter of solid particles in coarse sand is
2.000.20 mm, in ne sand 0.200.02 mm, in silt 0.020.002 mm and in
clay < 0.002 mm.
A sandy loam soil contains 66.6% coarse sand, 17.8% ne sand, 56% silt
and 8.5% clay. A loamy soil contains 27.1% coarse sand, 30.3% ne sand,
20.2% silt and 19.3% clay. Finally, a heavy clay soil contains only 0.9% coarse
sand, 7.1% ne sand, 21.4% silt and 65.8% clay.
Sandy soils have a large volume of non-capillary pore spaces and are
characterized by adequate aeration and drainage. Furthermore, sandy soils
have a low CEC and low water-holding capacity; they are easy to cultivate and
chemically inert. Clay soils have a high surface area and greater water- and
nutrient-holding capacity. The loamy soils contain about equal amounts of
sand, silt and clay and they have intermediate behaviour between clay and
sand. Moreover, such soils are more appropriate for olive cultivation, have good
water- and nutrient-holding capacity and good aeration.
Soil structure
Soil particles are united as aggregates in the form of crumbs, giving rise to the
particular soil texture. Clay particles are occulated when there is good
availability of cations in the soil solution, which neutralize the negative charge
of the clay particles. Hence, neighbouring particles stop repelling each other
and form aggregates in the form of crumbs. The role of iron and/or aluminum
oxides in organic colloids is important in cementing these particles together.
Wetting can destroy aggregation. Furthermore, the predominant ions may
affect soil structure and permeability. Soil saturated with sodium has a poor
structure: it is easily dispersed and does not drain easily.
Soil porosity
The pore space is that fraction of soil lled with air or with soil solution, and
this constitutes at least 50% of the soil volume. The pore space consists of two
types of pore, i.e. capillary and non-capillary. Capillary pores are lled with
65
water after drainage of the soil, while the non-capillary do not hold water and
drain easily after irrigation. Therefore, such pores are lled with air. Sandy soils
have good aeration and drainage, due to the non-capillary pores. However,
such soils have low water-holding capacity. An ideal soil for plant growth has
50% non-capillary and 50% capillary pores.
Soil horizons
Olive groves can be found in areas with a uniform soil texture to a depth of
1.01.5 m. Older, undisturbed soils have denite horizons with different
properties. These horizons are categorized as below, starting from the surface
of the soil:
AOO: under-composed organic debris.

AO: partly decomposed organic debris.
A1: high content of organic matter plus mineral material.
A2: light-coloured horizon with maximum leaching.
A3: transition zone to horizon B.
B1: similar to A3.
B2: zone of maximum illuviation.
B3: transition zone to mother rocks.
C: mother rocks.
Classication and retention of soil water

Soil water content is dened as the amount of water per unit of dry soil or the
volume of water per unit volume of bulk soil. However, this signies little with
regard to water availability for plant growth. Water is retained by different
mechanisms in various soils, such as pressure or tension applied to remove
water and water retention in the presence of osmotically active salts, which
lower the vapour pressure of the soil water. The chemical potential of soil water
is termed the soil water potential (soil).
Soil water potential
The forces that participate in the soil water potential are associated with the
soil, the osmotic pressure of the soil solution, etc. The forces associated with the
soil matrix are termed matric forces and constitute the matric potential (m). The
forces associated with soil solution constitute the osmotic potential (s), and
any applied pressure gives rise to the pressure potential (p). Therefore, the
water potential of soil water is the sum of three components:
soil = m + p + s
66
Chapter 8
In the case of when another force, such as soil gravity, is applied, the
previous equation is transformed into the following:
soil = m + p + s+ g
Soil water can also be described by two other terms: eld capacity and
permanent wilting percentage.
Field capacity
This is the water content after drainage 13 days after the soil has been
thoroughly wetted by rain or irrigation. Field capacity is a condition in which
water moves slowly and the water content does not change signicantly
between measurements. The deeper the soil the more time is required for the
surface layer to reach eld capacity. Furthermore, eld capacity depends on soil
characteristics and the condition under which it is measured. Because eld
capacity is a function of soil structure, laboratory measurements are not a very
good estimation of its value under eld conditions.
The moisture equivalent is an estimation of eld capacity, which is the water
content of a wetted and sieved soil sample having been drained by centrifugation
at a gravitational acceleration of 1000 g. In order to measure moisture
equivalent, soil samples are exposed on a pressure plate to a 0.3 bar pressure
and the soil moisture content is an approximation of the moisture equivalent.
Permanent wilting percentage
Permanent wilting percentage (PWP) is the soil water content at which plants
remain permanently wilted. PWP is the lowest limit of soil water and is
measured by cultivating seedlings in well irrigated containers until adequate
growth is achieved. Subsequently, the soil surface is sealed with plastic or
aluminum foil and the plants are allowed to deplete the available soil water,
leading to wilting. When no recovery of plants is recorded in a moist area then
the soil water content measured is termed PWP. The soil water potential at
wilting has a mean value of about 15 bars. Therefore, the percentage of water
content at 15 bars is used as an estimation of PWP. In soils PWP is determined
on soil samples by using a pressure membrane. The appearance of wilting in an
olive tree may be due to the inability of water supply from roots to meet the
transpirational demands of the tree. Other factors responsible for wilting in the
orchard include depth and soil volume where the root system grows, soil water
conductivity, osmotic stresses and other factors.
Readily available soil water
With the term readily available water we mean the amount of water retained in a
soil between eld capacity and the PWP. The available water range has a greater
value in ne-textured soils in comparison with that in coarse-textured. The value
of available water is a function of many variables, such as high rooting depth or
restricted root growth and rate of inltration of water.
67
Inltration of water and its movement into the soil

For maintenance of olive growth, soil moisture content should be restored by
rain and/or irrigation. When water is applied to the soil surface it moves
downward and creates ve zones, i.e. the saturation zone, a transition zone, the
main transition zone, a wetting zone and the wetting front. Water inltration is
affected by the initial water content, soil organic matter, colloids and surface
permeability, soil temperature and duration and intensity of the rainfall.
Existence of claypan or hardpan signicantly decreases soil inltration.
Surface permeability and hence inltration can be increased by the
addition of organic matter and its incorporation into the soil surface. Water
within the soil moves via the soil pore spaces. The driving forces include gravity
and the inuence of surface tension forces. Also, water could be diffused in the
form of vapour via the air space of soil. The direction of movement is towards
the lower water potential. In waterlogged soils (saturated ow) the driving
force is gravity, which controls the water potential gradient. In dry soils the
water potential gradient is controlled by the water potential and water moves
in the form of lms. As a soil becomes drier, the matric potential m is the main
force. According to hydraulic conductivity laws we can classify soils as poorly
drained when the k value (the property of a material that indicates its ability to
conduct heat) is less than 0.25 cm/h, and as well drained when k is greater
than 25 cm/h. Soils with a very high k value are not appropriate for olive
cultivation. The smaller the pore size the less the soil permeability. Another
means of water movement is in the form of vapour, when the soil water
potential is about 15 bars.
Soil temperature shows seasonal uctuation; the soil surface temperature
increases during the summer and becomes colder during winter. This helps
upward movement during summer, in the form of vapour.
Measurement of soil water content

Soil water content signicantly affects olive tree growth. Various methods of
measuring soil water content have been devised. Due to the variability of soil
texture and soil water distribution it is not easy to obtain an accurate picture of
the soil water content throughout the entire root zone. Therefore, soil
sampling should be repeated in order to give an accurate determination of soil
water content. The methods for determination of soil moisture may be direct or
indirect.
Direct methods of measuring soil water content
Soil samples of known weight or volume are collected and the water content is
expressed in either g H2O/g oven-dry soil or /cm3 of oven-dry soil. The samples
are collected in a specic type of container of known volume. The soil sample is
68
Chapter 8
oven dried at 105C to constant weight. The amount of water in the sample is
determined as the difference in the weight of the soil sample before and after
drying. The water content is determined as the percentage of dry weight of the
soil sample. Also, this can be expressed as water content per unit of volume.
Indirect methods of determining soil water content
NEUTRON PROBE This is the most commonly used indirect method of measuring
soil water. The principle of this method is that hydrogen (H2) atoms can slow
down and scatter fast neutrons. Therefore, counting of low neutrons near a
source of fast neutrons gives an estimation of H2 content of the soil. Since the
main source of H2 in a soil is H2O, this technique provides an estimation of soil
water content. However, in organic soils (organic material contains H2) this may
modify the estimation of water content by this method.
Water has a signicantly higher dielectric constant
than dry soil. Therefore, changes in water content change electrical capacitance,
which varies with temperature and is less affected by the soil solution salt
concentration. Since it is difcult to obtain uniform electrical contact with the
soil, capacitance measurements are neither accurate nor reliable.
ELECTRICAL CONDUCTANCE
As the water content of the soil decreases, heat

conduction via the soil is decreasing. Thermal conductivity can be used to
measure the water content of the soil, especially in moist and sandy soils. This
method uses a heating element buried in the soil; the element is heated by
electricity and the rate of heat transfer is measured.
THERMAL CONDUCTIVITY
ELECTRICAL RESISTANCE BLOCKS This method is based on the measurement of

electrical resistance as the soil moisture changes. The electrodes are embedded
in small blocks of plaster of paris (gypsum); the blocks are buried in the soil and
connected to a resistance bridge. The water content of the gypsum changes
with the water content of the soil and results in changes in electrical
conductivity of the soil solution between the electrodes. The range of matric
potential where gypsum blocks work is 0.5 to 15 bars. Therefore, gypsum
blocks are more appropriate in dry soils for soil water measurement.
Soil water potential measurement

Tensiometry
In salty soils the water potential is almost equal to matric potential, and
tensiometry provides measurements closely related to total water potential of
greater than 0.8 bar. However, in salty soils, the osmotic potential has a
signicant value and matric potential is not a good approximation of the total
water potential.
69
Freezing point depression

With cryoscopic methods the freezing point depression of soil water is
measured. Afterwards, the solute potential is calculated from the relationship
between chemical potential and freezing point depression.
Thermocouple psychrometry
The relative vapour pressure is measured by a thermocouple psychrometer.
This measurement is based on the relationship between the chemical potential
of water and the depression of vapour pressure.
Measurement of osmotic potential
Osmotic potential is measured within the soil solution following soil
saturation, its extraction from the soil and ltering. With this technique the
saturation extract is produced. The osmotic potential can be determined by
either cryoscopy or vapour pressure psychrometry. Furthermore, the electrical
conductivity of the soil solution is measured since there is close agreement
between osmotic potential and electrical conductivity.
Achievement of certain levels of soil water stress
Plants are the best indicator of the need for irrigation, and plant growth is
controlled by the water potential value of its tissue. The colour of certain crops
turns dark blue-green as water stress develops; this colour change is a good
irrigation criterion. Furthermore, as water stress develops the olive leaf angle
changes and leaf rolling is observed. Also, guard cells are very sensitive to
water stress and closure of stomata has been used as an indicator of the need
for irrigation. Indicator plants can be used to determine the need for irrigation.
The rate of growth of fruits, leaves and trunks (dendrometers) has been used as
an indicator of the needs of the olive for irrigation.
Waterlogging
The olive is a species sensitive to waterlogging or root hypoxia, and its
sensitivity is a function of olive cultivar (Hassan and Seif, 1990). Of the tested
cultivars cv. Mission died after a 30-day duration of waterlogging, while the
cv. Kalamon survived for a period of 60 days after waterlogging was imposed.
The mechanism of tolerance to waterlogging is based on the production of
adventitious roots near the soil surface. Some rootstocks, such as O. oblonga,
are tolerant to waterlogging. Waterlogging alters certain metabolic functions
of roots and results in reduced absorption and transport of water and nutrients
(Larson et al., 1991).
70
Chapter 8
GASEOUS POLLUTANTS
Signicant modications of the gas composition of the atmosphere have been
observed in recent years for sulphur dioxide (SO2), carbon dioxide (CO2) and
ozone (O3). These pollutants are present at high concentrations, and for several
months in the Mediterranean region, where the olive plant is mostly cultivated,
reduced growth was observed in annual plants and forest trees (Agrawal and
Agrawal, 2000). However, the data concerning pollutant effect on olive
growth and metabolism are scarce (Minnocci et al., 1995; Vitagliano et al.,
1999).
Sulphur dioxide
Experiments have indicated that differences in SO2 sensitivity were observed
between two olive varieties, Moraiolo and Frantoio (Giorgelli et al., 1994).
Due to EU regulations for the protection of the environment, emissions of SO2
in more recent years have been signicantly reduced.
Carbon dioxide
The CO2 concentration of the atmosphere has increased from 280 to 360
mol/mol in recent times and it is expected to reach 650700 mol/mol by
the year 2075 (Carbon Dioxide Information Analysis Center). Carbon dioxide is
responsible for most of the global greenhouse effect. Exposure of olive plants
(Frantoio and Moraiolo) to elevated CO2 enhanced net photosynthesis and
decreased stomatal conductance (Tognetti et al., 2001, 2002; Sebastiani et al.,
2002a). Chlorophyll concentration decreased in an elevated CO2 environment
in the leaves of only Frantoio. Stomatal density and leaf nutrients did not
differ between treatments.
Ozone
Ozone in the stratosphere protects the living world from exposure to UVB
radiation. In the Mediterranean region O3 concentration is around 40100
ppb and is an atmospheric pollutant. Ozone pollution effects on the varieties
Frantoio and Moraiolo were studied (Vitagliano et al., 1999). Of these two
varieties Moraiolo showed greater visible injury to O3 than did Frantoio.
Therefore, in Mediterranean olive culture, O3 pollution may decrease olive
growth and productivity (Minnocci et al., 1999; Sebastiani et al., 2002b).
71
LIGHT AND PHOTOSYNTHESIS

Light interception and photosynthesis
Olive orchards include both intensive and extensive olive cultivation. The light
interception in extensive orchards is 2030% of the solar radiation, while in the
new, intensive orchards light interception can be as high as 70%. Photosynthetic
active radiation (PAR) reaching the leaves is the critical factor for dry matter
accumulation. However, PAR is a function of light intensity, canopy morphology
(Norman and Welles, 1983) and its optical characteristics (Ross, 1981).
The arrangement of olive leaves within the whole plantation is a function of
the planting system, canopy design and orientation of tree lines in the orchard.
Therefore, the high and super-high density plantings are two-dimensional
systems, while the classical olive orchards are a three-dimensional system. Other
factors controlling PAR are the inclination of leaves and light transmittance and
reectance (Norman and Welles, 1983).
Effect of leaf development, senescence and abscission

on photosynthesis
Net assimilation of olive leaves increases with leaf area expansion and obtains
maximum Pn value when the leaves attain 40% of their nal size. Also, total
carbohydrate and chlorophyll content and leaf dry matter are a linear function of
leaf area. The leaf initially develops as a sink for carbohydrates and gradually
becomes a source (Foyer and Galtier, 1996). Therefore, initially the growing leaf
imports carbon from neighbouring organs and later becomes a carbon exporter.
The onset of carbon export depends on the position of each leaf along the shoot.
Olive leaves persist for 23 years and their age increases from the tip to the base of
the shoot and affects photosynthesis (Bongi et al., 1987). From the total number
of leaves a certain percentage abscises and is replaced by new ones. This means
that leaves of all ages are present on one tree at any one time. During leaf
senescence a change from anabolism to catabolism happens and the net
photosynthetic rate (Pn), stomatal conductance (Gs), respiration, chlorophyll
content, dry matter, starch and N decrease. As a leaf ages, 50% of the total N is
exported to other organs and parts of a tree before abscission; senescing olive
leaves contribute to carbon and N balance. Therefore, olive trees should be
trained appropriately to allow adequate light penetration of the tree canopy.
Effect of olive cultivars and season on photosynthesis

The photosynthetic rate is high during the period from spring to autumn. The
principal factors affecting Pn are: (i) radiation; (ii) water stress (Diaz-Espejo
72
Chapter 8
et al., 2006); (iii) salt stress (Bongi and Loreto, 1989); (iv) temperature (Bongi
et al., 1987); (v) adequate nutrients; (vi) disease control; (vii) leaf area and
anatomy; (viii) leaf inclination; and (ix) the concentration of the carbonxation enzyme, RuBisCo. However, olive productivity is a function of both Pn
and respiration of the various organs. The photosynthetic rate (Pn) is affected
by both the time of day and the season (Proietti and Famiani, 2002). Therefore,
the highest Pn values were recorded in October and the lowest ones in August
and December. The explanation is simple, since the temperature is favourable
during October for photosynthesis and unfavourable during August and
December. Both high and low temperatures affect chloroplast integrity, light
scattering and light reectance. Measurement of Pn in ve Greek olive cultivars
(Hagidimitriou and Pontikis, 2005) indicated that Koroneiki, a droughttolerant cultivar, had the highest Pn and Gs values (21 mol/m2/s), followed
by Megaritiki (18 mol/m2/s), Conservolia (16 mol/m2/s), Lianolia
Kerkiras and Kalamon (both 1314 mol/m2/s).
The Pn is signicantly higher in 1-year old leaves than current-season
leaves early in spring, while later current-season leaves can also supply equal
amounts of assimilates for plant and fruit growth (Bongi et al., 1987). Olive
leaves have a pronounced seasonal variation in Pn, and this is affected by the
cultivars, date and ambient conditions (Hagidimitriou and Pontikis, 2005).
Gas exchange of olive fruits: effect of fruit load

The dry matter of olive fruit increases gradually from fruit set to the end of
October. The oil content starts to increase 4050 days after full bloom up to the
period of initiation of fruit maturation. Dark respiration (RD) and stomatal
conductance (Gs) were both high after fruit set, and then decreased. Also, Pn
and chlorophyll were high in the rst 3 weeks after fruit set and progressively
decreased thereafter (Proietti et al., 1999). For a signicant part of the fruitgrowing period the CO2 intake by fruit permitted the reassimilation of 4080%
of the CO2 produced by dark respiration. As the olive fruit grows, Pn decreases
due to the reduction in the ratio between fruit surface area and volume.
Fruit load and the presence or absence of fruit on the shoot does not
signicantly inuence certain parameters such as Pn, Gs, internal CO2 (Ci),
transpiration rate (E) and respiration rate (RD). Furthermore, chlorophyll,
sugar content and water content were signicantly inuenced by fruit load. As
the fruit grows it becomes a great sink, which does not improve the leaf s
photosynthetic efciency.
Girdling and photosynthesis

An immediate reduction of 80% in photosynthesis was observed following
girdling (incising the bark through 360 to enhance fruiting). After healing of
73
the girdling area, photosynthetic activity was restored. The presence of fruits
on the shoot resulted in such a decrease not being observed. Girdling resulted
in a decrease in Gs. The decrease in photosynthesis due to girdling could be
ascribed to assimilate accumulation, implying that Pn should be controlled by
assimilate demand. Therefore, transport of assimilates from source to sink is
the controlling factor of sink growth and the Pn of the source plant. When the
production of photosynthates exceeds their utilization the accumulative sugars
in the leaves become a type of stress factor. Other effects of girdling include the
increase in both percentage of oil in the fruit and ower bud differentiation.
Elevated carbon dioxide and photosynthesis

Rising CO2 has the potential directly to alter plant growth via its effects on
gaseous exchange (Kirschbaum, 2004). Assimilation rates are enhanced
(Centritto, 1998) and Gs is reduced for plants grown in high CO2. Rising CO2
resulted in a persistent increase in Pn, as happens with the majority of the C3
plants (see above) exposed to elevated CO2. The decline in Gs between elevated
and ambient CO2-grown plants was a result of short-term stomatal response to
CO2. Furthermore, as a result of changes in Pn and Gs, water use efciency
(WUE) increased under elevated CO2. Water use efciency can be dened as
either the ratio of CO2 assimilation to water lost via transpiration or the ratio of
net CO2 assimilation rate to transpiration.
Water stress and photosynthesis

Maximum values of Pn rates are reached early in the morning in both control and
water-stressed plants, and subsequently declined gradually. This photosynthetic
inactivation was accompanied by changes in the uorescence characteristics of
the upper leaf surface. In the water-stressed plants the maximum uorescence (Fp)
and the ratio Fv:Fp (variable uorescence (Fv) decreased by midday. However, the
initial uorescence (F0) rose to a maximum value at midday and declined in the
afternoon. In the control plants the Fp and the ratio Fv:Fp increased again in the
afternoon and had recovered completely by 20.00; in the stressed plants the ratio
Fv:Fp declined gradually as water stress developed.
CLIMATE CHANGE AND ITS EFFECT ON OLIVE CULTURE

General
The fundamentals of world climate include:
The strength of the incident radiation from the sun, which controls the
Earths overall temperature.
74
Chapter 8
The greenhouse effect of water vapour and other gases.

The energy from the sun which the Earth receives is 1370 W/m2, and, since
the Earth is spherical, each m2 receives about 342 W. The majority of this
incoming solar radiation is absorbed in equatorial regions, while
considerable quantities of radiation are emitted from the polar regions. The
oceans cover 71% of the Earths surface and play a signicant role in heat
redistribution, and its heat capacity in comparison with the atmosphere is
huge.
The greenhouse effect

Atmospheric air is constituted by O2, N and a number of minor gases such as
water vapour, CO2, O3 and nitrous oxide (N2O). Oxygen and N are transparent
to both solar radiation and infrared radiation, the latter being emitted from the
Earths surface. Furthermore, the minor atmospheric gases are transparent to
solar radiation and absorb the infrared radiation that the soil surface emits.
The main components identied with the greenhouse effect are illustrated in
Fig. 8.8.
Water vapour content varies from < 0.01% by volume to > 3.00%. Other
gases include CO2, methane, N2O, O3 and halocarbon compounds such as
O3-depleting chlorouorocarbons and hydrouorocarbons. The previously
mentioned gases absorb radiation and re-emit some radiation towards the soil
surface, leading to its warming. In the absence of the greenhouse effect the
Earth would maintain a temperature of 18C, while with the presence of
greenhouse gases the average temperature is 14C. Most of this 32C difference
Fig. 8.8. Percentage participation of CO2, water vapour and other factors involved
in the greenhouse effect.
75
in temperature is due to water vapour (21C) and CO2 (7C), while O3

contributes 2C. The presence of greenhouse gases in the atmosphere is
essential to life on Earth, allowing it to maintain an average surface
temperature between the boiling and freezing points of water.
Carbon dioxide
Carbon dioxide is released into the atmosphere through both natural and
human processes. Carbon dioxide production and absorption take place
through the carbon cycle in the biosphere. Human processes such as fuel
burning increase the CO2 concentration in the atmosphere; this has increased
by 30% from the late 18th century up to modern times, and is now at a level of
about 370 ppm, while it was recorded at 280 ppm before the Industrial
Revolution. The stomata of leaves therefore play a role in sensing and driving
environmental change (Hetherlington and Woodward, 2003).
Methane
Methane concentration has increased by about 150% since 1750. Methane is
a greenhouse gas produced from both natural and human activities.
Anthropogenic factors result in greater emissions of methane. The level of
atmospheric methane increased from about 1610 ppb in 1983 to 1745 ppb in
1998.
Why climate change is happening

Climate change is happening fast and it is caused by the human input of greenhouse gases into the atmosphere. Climate changes in the past were slow,
allowing plants and animals to adapt.
During the 20th and 21st centuries the average global temperature has
increased to levels not existing in the previous 650,000 years of Earths history.
The global temperature rose by 0.7C during the last century and has already
risen by another 1.6C, due to increasing concentrations of greenhouse gases
in the atmosphere. However, olive varieties have certain heat requirements in
order to ower, and various classical models could be used in order to evaluate
their heat requirements (Ribeiro et al., 2006). The CO2 concentration has been
constant to 280 ppm for the last 1000 years but, due to industrial growth, has
risen to 370 ppm today. Besides CO2, other gases are increasing in
concentration. Methane emissions constitute 15% of greenhouse gases today
and are going to increase to 50% by the year 2100. This intensies the
warming effect, being equivalent to 430 ppm of CO2. This increase in
76
Chapter 8
atmospheric greenhouse gases is due to human activity, mainly through

accumulation of greenhouse gases due to the burning of fossil fuels to cover
human needs. Other signicant factors are deforestation, farming methods
and poor waste management. Over the previous 140,000 years the climate has
been harsh and uctuating; however, over the last 8000 years the climate of
our planet has been stable, leading to agricultural growth and therefore
human civilization as we know it.
Side effects of climate change

At the present time the climate is 0.7C warmer than before industrialization
and has caused the following signicant changes in our planet (IPCC, 2001).
1. Melting of polar ice and glaciers, leading to a sea level rise of 0.2 m.
2. Unpredictable and violent weather changes (see Fig. 8.9).
3. Heatwaves (see Fig. 8.10) and extended drought and ooding (Rodriguez
Diaz et al., 2007). It is predicted that CO2 concentration in the atmosphere is
going to rise to 550700 ppm by the year 2050 and to 6501200 ppm by the
year 2200. This will lead to global warming of 2.5C or even more by the year
2050. The polar regions will have more severe levels of warming, which will
lead to sea levels rising by > 0.5 m, creating problems for millions of people.
Such climate changes will create water shortages for 4 billion people, and
melting glaciers will create ood risk and displacement from their areas for 200
million people.
Fig. 8.9. Storm frequency in the northern hemisphere due to climatic changes over
the last 100 years (from Lambert, 1996).
77
Fig. 8.10. Expected change in extreme high temperatures, from the present day to
the year 2090 (from Hangeveld, 2000).
4. The decreased water supply, together with rises in temperature, will decrease
food supplies.
5. Ocean acidication with increased CO2 concentration has negative effects
on and consequences for sh populations and marine ecosystems.
6. Increase in deaths due to heat stress, lack of food, malaria, etc.
7. Increase in the frequency of very intense hurricanes, typhoons and tropical
storms.
8. Loss of an increasing percentage of biodiversity, since various species fail to
adapt to harsh weather conditions. A temperature rise of 2C will lead to
extinction of 1540% of species that are a source of food and medicines.
9. Changes in sea level. The rate of global mean sea level rise during the 20th
century was in the range of 1.02.0 mm/year. The most rapid rise in global sea
level was between 15,000 and 6,000 years ago, with an average rate of
10 mm/year.
IMPACTS OF CLIMATE CHANGE ON OLIVE TREES

Cycle length
Higher temperatures in late winter and early spring will promote the
developmental stages of olives. With respect to olives no experimental results
are known, but it can be expected that the owering season will be promoted
78
Chapter 8
(Frenguelli et al., 1989; Gioulekas et al., 1991; Chuine et al., 1998; Galn et al.,
2005). However, higher temperatures could be catastrophic to owering
quality (perfect owers) and therefore it could reduce olive production.
Higher temperatures increase biomass production. Furthermore, in olives
and in other woody species, the inuence of changing climate will depend on
how long the increased CO2 concentrations promote growth and production
and also how the inuence of CO2, temperature and non-availability of water
affects olive fruit and quality.
Weeds, diseases and pests

Greater CO2 concentrations promote weed growth. The competition for olive
plants from weeds will depend on their respective reactions to climate change.
However, the nature of damaging effects depends on the weed species.
Increasing temperature in the northern hemisphere leads to increased air
humidity and disease growth, such as the fungus Cycloconium oleaginum,
which can cause considerable commercial damage to plantations. The risk of
olive damage from pests and diseases has increased under warming of the
climate; more generations of pests and diseases can be expected. This implies
an increased requirement for pest and disease control, with negative effects
on the environment due to agrochemical pollution. Furthermore, O3 concentrations are increasing in warmer temperatures, and high O3 concentration
may lead to yield losses. Pesticide use is projected to increase by 1020% for
olives under climate warming. This increase in pesticide use leads to reduced
economic prot.
Modication of culture areas

The olive tree is a Mediterranean crop. However, climatic changes will create
desertication of the Mediterranean region and will render the temperatures of
more northerly countries more benecial (Parry, 1992). Therefore it is now
possible to plant an olive grove in the UK, where already Mark Diacono has
planted his grove of 120 olive trees along the banks of the River Otter and is
expected to produce the UKs rst olive oil within a few years. Furthermore, the
seasons will change and summer and winter will be extended (Sparks and
Menzel, 2002).
Organic olive farming impacts on climate change

Many people do not realize that agriculture is a signicant contributor to
atmospheric CO2. Furthermore, the various types of agriculture produce
79
varying amounts of CO2. Organic olive culture controls weeds by ploughing,

which doubles CO2 emissions from the soil compared with non-tillage culture.
Conventional olive culture relies on synthetic fertilizers while organic olive
culture uses primarily manure. The use of manure fertilizers increases soil
respiration rates, and therefore CO2 emissions, by two- to threefold. Some
researchers suggest a complete conversion to organic agriculture. However,
this conversion requires a tremendous increase in the numbers of agricultural
animals to produce manure fertilizer, which would result in a signicantly
greater CO2 production in comparison with conventional olive culture.
Today, the worldwide tendency is to plant more olive trees, even in
countries not traditionally cultivating the crop. This greater number of olive
trees helps to restore the productivity and richness of the soil. Olive trees also
absorb CO2 from the environment and ght the symptoms of global warming,
which has now become the Earths biggest threat in the 21st century.
Climatic impact on olive irrigation

The most recent estimate of climate change in Europe suggests an increase in
temperature of up to 0.7C per decade during summer and, furthermore, a
reduction and change in the distribution of precipitation during the 21st
century (Moreno, 2005). The available irrigation water is going to decrease
while water demand is going to increase. The reduction in available water will
be greater in the southern Mediterranean areas. Also, the frequency of days
with temperature extremes, droughts or storms is going to be signicantly
increased.
With regard to CO2, its concentration is increasing, leading to greater
productivity (see Fig. 8.11). This positive effect is counterbalanced by the
higher temperatures and lower rainfall that increase the olives water requirements. The irrigation seasons are also now longer, due to lower rainfall.
Climate change requires that irrigation systems will have to be designed for
longer and higher peaks.
Climatic change and olive tree phenology

Olive phenology is a sensitive indicator of climate change and especially of the
future climatic warming of areas where olives are grown (Osborne et al.,
2000). By phenology we mean the study of the timing of periodic biological
and/or natural events, and especially during the months of spring.
Rising spring temperatures during the previous century have advanced the
time of emergence of owers on olive trees. Several models based on thermal
time and chilling have been developed for predicting the owering date of olives.
80
Chapter 8
Fig. 8.11. Actual plus estimated increase in atmospheric CO2 concentration over a
period of 115 years (19752090).
Their analysis shows that the best predictions are from thermal time models
based only on spring temperatures (Chuine et al., 1998). The annual owering
date for an individual site may vary by 2930 days within a single decade.
Phenology is the most responsive aspect of nature to warming, and is an
ideal means of demonstrating that warming may already have an inuence on
the natural world. Olive owering and deciduous tree leang are amongst the
easiest processes to record. Furthermore, in deciduous trees any increase in the
mean temperature leads to advancement of harvesting date. Planetary
warming and higher spring temperatures affect the timing of bird migration
and breeding periods.
Adaptability to climate change

Adaptational measures are likely to be critical and include the following:
Breeding for high CO2 concentration and selecting for CO2 response.
Breeding for tolerance to water and heat stress. Certain olive varieties have
proved to be more tolerant to high temperatures or to water stress conditions.
Selection for tolerance to diseases and insects, since an increase in the use
of pesticides and herbicides could threaten drinking water quality.
WEB SITE
Carbon Dioxide Information Analysis Center: https://cdiac.ornl.gov/
9
FLOWER BUD INDUCTION
AND DIFFERENTIATION
INTRODUCTION
Flower bud inorescences are borne in the axil of each leaf. Usually the bud is
formed on the current seasons growth and begins growth the following
season. Lombardo et al. (2006) report observations regarding the oral biology
of 150 Italian olive cultivars. Buds may stay in a dormant condition for more
than 1 year and subsequently begin growth, giving inorescences bearing
owers. Each inorescence contains 1530 owers and this depends on the
cultivar and the prevailing conditions for development.
The owers are small, yellowish white and inconspicuous and contain a
four-segmented calyx and a tubed corolla with four petals (lobes). The ower
also has two stamens and a short style with a two-loculed ovary. The owers
are divided between two categories: perfect, having stamen and pistil, and
staminate (male) owers, where the pistil is aborted while the two stamens are
functional. In the perfect ower the pistil is large, green in colour and lls the
space in the oral tube. Staminate owers are very small and do not ll the
oral tube; the style is greenish white and small.
Flower initiation takes place very early in November and the ower parts
develop during MarchApril, as has been shown by histochemistry. Induction
in the olive may occur about 6 weeks after full bloom, while the initiation is not
visible until 8 months later (February). Various authors report on the time of
oral induction (Hartmann, 1951; Fernndez-Escobar et al., 1992; Cuevas et
al., 1999).
For ower induction, chilling is a prerequisite. Some olive cultivars
(Koroneiki, Mastoidis) in southern Greece require very little chilling, while
other cultivars (Kalamon, Amphissis and cultivars from California and
Spain) require adequate chilling. Experiments in Thessaloniki indicated that
the cultivars Chondrolia Chalkidikis and Amphissis need longer periods of
chilling than do Megaritiki and Koroneiki to satisfy their chilling requirements for ower induction (Hartmann and Porlingis, 1957; Hartmann and
Whisler, 1975; Porlingis and Therios, 1979). The rst appearance of sepal
81
82
Chapter 9
primordia occurs during the rst 12 days of April, the beginning of carpel
differentiation was observed during the last 10 days of April and full bloom
took place during the last few days of May or the rst days of June (Porlingis
and Dogras, 1969).
Olive inorescences appear during spring and originate from buds produced
during the previous years vegetative period. Initially, these buds are not
differentiated to owers. However, after exposure to low winter temperatures,
internal changes occur in endogenous inhibitors and promoters, leading to their
differentiation to owering buds and to inorescences (Hartmann et al., 1967).
Exposure of buds to relatively low temperatures is the necessary condition for
owering, and both the level of low temperatures and the cultivar determine to a
great extent the percentages of buds that will produce inorescences.
STAGES OF FLOWERING BUD INDUCTION AND

DIFFERENTIATION
Observations with the cultivar Chondrolia Chalkidikis under the climatic
conditions pertaining in the area of Thessaloniki, Greece and also of other
papers (Hackett and Hartmann, 1963, 1967) indicate that the owering
process of the olive can be divided into the following stages (Porlingis and
Dogras, 1969).
1. The stage of owering bud induction, which starts in October and lasts up to
the end of February. The vegetative buds are subjected to winter chilling, which
causes physiological changes necessary for owering bud induction. However,
these buds remain unaltered morphologically.
2. The stage of morphological changes, which leads to the development of a
central axis and side branches of the inorescence and the meristems destined
to develop into owers. The duration of this stage for the climatic conditions of
Thessaloniki is about 40 days. For this stage low temperatures exert a
benecial inuence.
3. The stage of differentiation of owers. Initially the induction of sepals takes
place and is followed by petals, stamens and carpels. The induction takes place
the rst 15 days of April, while that of carpels after 1620 days.
4. The stage of completion of growth of the various parts of owers. The full
bloom happens from the end of May to the rst days of June. The duration of the
3rd and 4th stages is 4560 days (see Figs 9.1, 9.2 and 9.3). Figure 9.4 gives
the biennial cycle of vegetative and reproductive processes in the olive (Rallo et
al., 1994).
The olive inorescence is a panicle. The number of owers per inorescence
is a function of the cultivar, soil moisture during its development (Hartmann
and Panetsos, 1962) and N content of leaves. Prolonged and very cold weather
during April and May, when the olive ower buds should be developing rapidly,
Flower Bud Induction and Differentiation
83
Fig. 9.1. First stages of oral differentiation in olive buds (cv. Chondrolia Chalkidikis).
At the upper right of each microphotograph the date of bud collection is given.
1, vegetative bud; 2, 3, no change is observed; 46, the apical meristem becomes
broader and a new pair of bracts is formed (B1). K, apical stem; , lateral stem;
B, bracts (magnication 30) (from Porlingis and Dogras, 1969 [in Greek]).
has a detrimental inuence on owering, pollination and fruit set. From the
olive owers optimum yield can be achieved when 1 or 2% of these owers
remain as developing fruit 14 days after full bloom. Most of the owers not
setting fruit have abscised.
Olives are polygamousmonoecious since, on the same tree, we may nd
both perfect owers with weak, well-developed ovaries and staminate
(imperfect owers with well-developed stamens and underdeveloped ovary)
(Levin and Lavee, 2005).
The percentage of perfect owers on a tree depends on: (i) the cultivar; (ii)
shoot vigour; and (iii) environmental conditions, such as the lack of soil
moisture during spring, when the inorescences develop. Furthermore, the
leaf:bud ratio affects the percentage of perfect owers. When the leaf:bud ratio
is increased by disbudding, the production of perfect owers is promoted.
Constant temperature (12.5C) or ideal temperature variations during the
24-h period (12.5C for 20 h and 21C for 4 h) promote the development of
imperfect owers, whereas low temperatures or when the temperature varies
(7C for 20 h and 26C for 4 h) increase the percentage of perfect owers.
84
Chapter 9
Fig. 9.2. Further stages of oral differentiation in the olive bud (cv. Chondrolia
Chalkidikis). 7, 8, broadening of the apical meristem and appearance of the
primordial sepal (
); 9, sepal and petal () primordia in the apical meristem; 10, 11,
stamen differentiation (
); 12, carpel differentiation (K) (magnication 20) (from
Porlingis and Dogras, 1969 [in Greek]).
FACTORS AFFECTING FLOWERING BUD INDUCTION

Light
Light is an important factor in ower induction; shading reduces ower bud
differentiation (Tombesi and Cartechini, 1986). Shading following ower bud
differentiation does not affect owering, however, it can cause morphological
sterility or abortion of the ovary. Shading or leaf removal may postpone
owering.
The light intensity and quality are reduced as light passes through the top
of olive trees and varies according to the shape of the trees and the density of
their leaves. The index LAS (leaf area:soil area) is 2.5 (Bongi and Palliotti,
1995). The maximum net assimilation of CO2 under optimum conditions is
less than that in other C3 (see Chapter 8) plants. The Pn of olive leaves is
18 mol CO2/m2/s. Shotberries are parthenocarpic fruits that occur
85
Fig. 9.3. Pistil development in the apical ower of the olive inorescence. 13, pistil
formation; 1417, formation and development of ovary, ovules (
), style (
) and
stigma (
I) (13, 14 and 15: magnication 20; 16, 17: magnication 15) (from
Porlingis and Dogras, 1969 [in Greek]).
randomly in the form of clusters on each inorescence; these mature much

earlier than normal fruits.
Juvenility
Juvenility is generally dened as the period during which a plant cannot be
induced to ower. The duration of the juvenile phase in woody plants is quite
variable, and can be quite lengthy (Meilan, 1997). Phase change describes the
period during which a plant undergoes the transition from juvenility to
maturity. This transition is a gradual and continuous process. Phase change
has occurred if the olive plant owers independently of the cause of the
induction of owering.
The juvenile and mature growth phases are often distinguishable by other
morphological characteristics, which in olives can include leaf shape and size,
phyllotaxy and the ability to form adventitious roots and/or buds. The term
maturation describes the transition from juvenility to maturity, while ageing
means loss of vigour and increased complexity. In assessing the ability to ower,
86
Chapter 9
Fig. 9.4. Biennial cycle of vegetative and reproductive processes in the olive (from
Rallo et al., 1994). Dorm, dormancy.
it is necessary to use treatments that are known to induce owering but which
do not promote maturation.
Temperature
Winter chilling
Several climatic factors are apparently critical for olive performance, with
respect to owering, fruit growth and olive oil production and quality. Some
winter chilling is required for oral initiation, but this requirement varies
quantitatively between varieties (Hartmann and Porlingis, 1957; Hackett and
Hartmann, 1963, 1964, 1967; Navarro et al., 1990; Piney and Polito, 1990;
Ferrara et al., 1991; Rallo and Martin, 1991). Varieties originating from Italy,
Spain, northern Greece and California require more winter chilling than those
grown in southern Greece (Crete), Israel or Egypt (Martin et al., 1994a), where
the chilling periods range from 86 to 1400 h (Hartmann and Whisler, 1975).
Temperature uctuation affects owering: uctuation between 2 and 15C for
80 days produced adequate owering, while uctuation between 7 and 18C
produced smaller numbers of inorescences.
A chilling period is required prior to owering. Afterwards, the determination of heat requirements in the rst developing phases of plants has been
87
expressed as temperature accumulation by means of parameters such as Heat

Units, which are usually expressed as Growing Degree Days (GDD). Their
determination is useful for forecasting when owering will occur. The most
suitable threshold temperatures were carried out in a range of 715C. The
causes responsible for varying threshold temperatures recorded in areas of the
same latitude include different climatic conditions and the various cultivars
present within olive groves on the same latitude.
Variation of temperature and cultivars
Due to the late blooming of olives, ower damage due to late spring frosts is
rare. On the contrary, hot and dry winds during owering reduce fruit set.
Strong winds in June can increase the percentage of fruit drop.
In an experiment, olive plants grown in containers of the varieties
Koroneiki, Kolovi and Chondrolia Chalkidikis were exposed continuously
to temperatures of 10C, 14C, outdoors, outdoors + 10C and outdoors +
14C (Porlingis and Therios, 1979). After temperature exposure measurements were made of the number of inorescences, the percentage ower bud
differentiation, the number of owers per inorescence, the percentage of
perfect owers and the number of fruits/100 perfect owers. These data are
presented in Tables 9.1 and 9.2.
The data indicate that low temperatures exerted a favourable effect during
both the rst and the second stages of owering bud differentiation in Koroneiki,
but in Chondrolia Chalkidikis only during the rst stage. These observations
indicate the existing relationship between temperature and number of owers
per inorescence. The fact that the variety Kolovi has a constant number of
owers per inorescence at all temperature treatments indicates that the
response to low temperatures is cultivar dependent. The effect of temperature on
the percentage of perfect owers is not clear. Therefore, the level of temperature
during the rst stages of ower bud differentiation affects, at least in some
cultivars, the percentage of ower buds and number of owers per inorescence,
with no effect either on pistil development or the percentage of perfect owers. In
some olive cultivars, such as Arbequina, owering and fruiting can be achieved
in the absence of chilling temperatures ( 7C) (Malik and Bradford, 2005,
2006a). The absence of owering in subtropical climates is not due to lack of
chilling but due to high temperatures during winter days. Hence, temperature
during the day 23C could produce adverse effects on owering.
Growth regulators
The levels of ABA, IAA and GA3 in leaves, nodes and fruits during the
induction, initiation and differentiation periods in the on years (those with a
high percentage of owering and fruit set) were lower than those in the off years
(those with a low percentage of owering and fruit set in the alternate bearing
88
Chapter 9
Table 9.1. Number of inorescences per plant, percentage of ower buds and
number of owers per inorescence of three olive varieties maintained at various
temperatures (from Porlingis and Therios, 1979).
Variety
Treatment
Inorescences/plant (n)
Outdoors
10C
Outdoors + 10C
14C
Outdoors + 14C
Koroneiki
1343
1174
1524
924
1302
Kolovi
228
24
171
11
99
Chondrolia
Chalkidikis
1150
200
765
0
0
Flower buds/inorescence (%)

Outdoors
10C
Outdoors + 10C
14C
Outdoors + 14C
76.6a
75.7a
84.9a
56.2b
73.4a
15.8a
2.0c
12.0a
0.9d
4.5b
77.5a
12.7b
48.8a
0.0c
0.0c
Flowers/inorescence (n)
Outdoors
10C
Outdoors + 10C
14C
Outdoors + 14C
18.7a
18.1a
18.3a
14.6c
15.8b
7.6a
7.0a
6.9a
7.6a
8.3a
12.9a
8.6b
12.4a
Different superscript letters within the same column indicate statistically signicant
differences for P < 0.05 (Duncans multiple range test).
cycle). However, GA3 levels during these periods were higher in the on years.
The fact that GA3 decreased and GA4 levels increased during the induction and
initiation periods in the off years suggests that these affect ower bud
formation. Zeatin increase during the induction period in the off year suggests
that an increase in cytokinin during the induction period possibly has a
positive effect on oral formation (lger et al., 1999, 2004).
The ABA content of the inorescences was consistently > 40 nmol/100 g
fresh weight (FW) from early anthesis until full bloom, reaching a maximum
1 week before full bloom. The ABA concentration changes are thought to be
due to the shedding of bracts and imperfect owers during the period of
inorescence development prior to full bloom. After full bloom, endogenous
ABA concentrations fell to < 20 nmol/100 g FW. The ABA content of bark
tissues was about 30 nmol/100 g FW before full bloom. Shedding of old leaves
led to an increase in ABA concentration in bark tissues in late May. Exogenous
application of GA3, asparagine and glutamine affected the induction of
owering buds (Proietti and Tombesi, 1996a).
89
Table 9.2. Number of perfect owers and the number of fruits per plant and per
100 perfect owers of three olive varieties maintained at various temperatures (from
Porlingis and Therios, 1979).
Variety
Treatment
Koroneiki
Perfect owers/plant (%)

Outdoors
10C
Outdoors + 10C
14C
Outdoors + 14C
Perfect owers/plant (n)
Outdoors
10C
Outdoors + 10C
14C
Outdoors + 14C
77.3a
31.9b
35.6b
62.7a
43.5b
1759
1597
1935
1580
1732
Fruit (n)/100 perfect owers

Outdoors
10C
Outdoors + 10C
14C
Outdoors + 14C
9.1
23.6
19.5
18.6
19.4
Kolovi
90.3a
87.9a
90.0a
98.7a
96.5a
60
147
186
16
29
3.8
11.4
17.5
19.0
3.7
Chondrolia
Chalkidikis
84a
90.3a
69.7a
301
66
211
0
0
2.4
4.5
3.2
Sugars and nutrient elements

Differences in concentration of any of the sugars, with the exception of
fructose, were not signicant in the on and off years (lger et al., 2004). The
highest macro- and micro-element concentrations were found to be Ca and
iron (Fe), respectively. The results suggest that carbohydrates and mineral
nutrients may not have a direct effect on inducement of ower initiation (lger
et al., 2004).
Oleuropein levels
Oleuropein levels sharply decreased during the transition from vegetative to
ower buds (Malik and Bradford, 2006b), while levels rapidly increased with
the expansion of fertilized pistils and then sharply declined with fruit
maturation. Exogenous application of oleuropein inhibited owering in
90
Chapter 9
Kalanchoe blossfeldiana (Bongi, 1986). Therefore, oleuropein could play a role in

owering in olives. Drastic reduction in oleuropein levels was observed in oral
buds, as compared with vegetative buds.
After oral bud differentiation, oleuropein levels progressively increased
from the owering to fruiting stage and then declined as the fruit started to
mature, and were negligible in the fully mature black fruits (see Table 9.3).
Date of harvesting
The date of harvesting affects signicantly the percentage of ower bud
differentiation. Late harvesting depletes the olive tree and results in a lower
percentage of ower bud differentiation (see Table 9.4 and Fig. 9.5).
Fruit thinning
Olive fruit thinning increases the percentage of owering bud differentiation
and owering. Use of NAA (the auxin, naphthalene acetic acid) at 150 mg/l
increased owering bud differentiation by 60%.
Table 9.3. Sugar, hormone and mineral nutrient concentrations of Memecik olive
at the three oral stages (lger et al., 2004).
Sugar
Fructose (%)
Glucose (%)
Sucrose (%)
Total sugar (%)
IAA (g/g)
Zeatin (g/g)
ABA (g/g)
GA3 (g/g)
GA4 (g/g)
N (%)
P (%)
K (%)
Ca (%)
Mg (%)
Fe (g/g)
Mn (g/g)
Zn (g/g)
Cu (g/g)
Differentiation
Induction
Initiation
0.18c
3.08a
0.23b
3.48b
0.72b
18.09a
0.47a
4.12a
40.62a
1.04a
0.11c
0.59c
2.06a
0.17a
93.77a
33.82a
21.61a
29.13a
0.34a
3.26a
0.36a
3.99a
0.70b
5.42c
0.21b
1.53b
23.70b
0.97b
0.12b
0.91a
1.30b
0.13a
63.98b
22.73b
16.95b
17.77b
0.26b
3.12a
0.37a
3.81ab
1.75a
8.63b
0.15b
3.88a
41.36a
0.61c
0.14a
0.73b
1.98a
0.17a
60. 09b
19.20b
11.21c
16.04b
91
Effect of defoliation
The percentage of ower bud differentiation decreased after defoliation due to
diseases such as Cycloconium and nutrient and water stress.
Table 9.4. Flower bud differentiation (%) as affected by the date of harvesting and
fertilizer applied.
Date of harvesting
30 November
15 December
25 January
14 March
Fertilizer (urea)
Fertilizer (101010)
Control
6.88
4.05
0.85
0.46
3.48
1.31
0.29
0.20
3.33
1.63
0.81
0.09
Fig. 9.5. The relationships between (a), olive production (kg) and owering
intensity the following year; (b) harvesting date and ower bud differentiation the
following year (from Gucci and Cantini, 2004a).
10
FLOWERING, POLLINATION,
FERTILIZATION AND FRUITING
OLIVE FLOWERING AS AFFECTED BY GLOBAL

CLIMATIC CHANGE
Olive ower phenology is characterized by an annual cycle including bud
formation during the previous summer, dormancy during the cold period, bud
burst in late winter and ower structure development from bud burst to
owering in spring. Among the factors affecting plant development,
temperature and photoperiod exert the strongest effect on owering.
Furthermore, variations in temperature and photoperiod due to altitude and
latitude produce geographically differing growth phases.
Olive phenology is an indicator of global climatic change, especially during
spring months (Osborne et al., 2000). The rising of spring temperatures during
the past and current centuries has advanced the timing of leang and
owering in many species at high, northern latitudes. After chilling and
breaking of bud dormancy the subsequent rate of bud development depends on
the duration and warmth, usually known as heat sum or thermal time. In
olives, owering occurs between April and June in the Mediterranean region.
The owering date in olives may thus be a direct, simple and sensitive biological
indicator of climatic warming. Model simulations indicate that the owering
date for olives in the Western Mediterranean could become signicantly earlier
by the year 2099 (Osborne et al., 2000). The 4.5C rise in temperature
produced by greenhouse gases advanced owering at a rate of nearly 30 days,
or 6.2 days/C. Furthermore, future monitoring of airborne olive pollen may
therefore provide an early biological indicator of climatic warming in the
Mediterranean. This model predicts that phenology will advance more
strongly in large parts of southern France, Algeria, Tunisia, Morocco, southern
Spain and Portugal than on the islands of the Mediterranean.
93
94
Chapter 10
MODELS FOR THE PREDICTION OF FLOWERING DATE

Many previous studies on the prediction of owering have used data from only
one location (Frenguelli et al., 1989; Alcal and Barranco, 1992) and/or used
a simple thermal time approach (De Melo-Abreu et al., 2004). Model 1 is a chillheating model, which is a generalization of the Utah model. Model 2 is a < 7C
chill-hours model and model 3 relies on a thermal time approach after
1 February. Model 1 is more appropriate for predicting the time of owering in
the olive and it is the only model considered in this study that is physiologically
meaningful. Models 2 and 3 are also good predictors of the time of owering
but should not be used in warm areas, where some varieties do not satisfy their
chilling requirement. Figure 10.1 gives the phenological stages of olive trees
from inorescence development to the third stage of fruit growth.
POLLEN AND ITS CYTOPLASMIC STERILITY

Two types of male sterility are distinguished, according to their mode of
inheritance: (i) nuclear or genic male sterility (gMS); and (ii) cytoplasmic male
sterility (CMS) (Besnard et al., 2000). In most cases gMS is determined by a
single locus and is due to a recessive allele. The olive is an allogamous,
hermaphrodite and usually a wind-pollinated plant. Most of its cultivars are
self-incompatible, a trait descended from oleaster, but some such as Oliviere,
Chemlali and Lucques are totally male-sterile. This implies the necessity of
using another variety as pollinator (Besnard et al., 2000).
Compatibility between cultivars could reduce the problems of self-sterility
that have been observed in olives. Emission of pollen at differing times could
lead to lower reproductive success, since self-sterile cultivars may not be
receptive when pollen is either released from other cultivars or pistils have
passed their optimum receptivity, leading to reduced fertilization. Greater
homogeneity could result in pollen emission throughout pistil receptivity and
may result in more frequent cross-pollination between different self-sterile
cultivars, resulting in earlier and higher levels of fertilization and an increase
in total production. Farinelli et al. (2006) in their work have proposed the most
appropriate pollinators for 21 olive cultivars (see Table 10.1).
Differences between various olive cultivars were observed regarding the
onset and the start and length of owering period. These differences are strictly
correlated to differences in micro- and megagametogenesis and are cultivarspecic characteristics.
ANTIOXIDATIVE ENZYMES IN POLLEN

The mature pollen grains are released into the environment and are exposed to
a number of agents such as high or low temperatures, pathogens, air pollutants
Flowering, Pollination, Fertilization and Fruiting
95
Fig. 10.1. Phenological stages of the olive tree. (a) Inorescence development; (b)
ower enlargement (the owers become spherical); (c) full bloom; (d) petal fall and
imperfect owers; (e) fruit set and 1st stage of growth; (f) 2nd stage of fruit growth
(hardening of endocarp begins); (g) 3rd stage of fruit growth (cell enlargement).
and UV radiation. The enzyme superoxide dismutase (SOD) has been found in
olive pollen and catalyses the disproportionation of superoxide radicals (O2),
which is a defensive mechanism against oxidative stress (Aloh et al., 1998).
SOD occurs in three forms, containing manganese (Mn), Fe or Cu.
96
Chapter 10
Table 10.1. The most appropriate pollinators for 21 olive cultivars (Farinelli
et al., 2006).
Main cultivar
Most appropriate pollinator(s)
Arbequina
Ascolana semitenera
Ascolana tenera
Bella di Spagna
Carolea
Carolea, Kalamon
Carolea, Itrana, Kalamon, Picholine
Maurino
Nocellara etnea
Ascolana semitenera, Bella di Spagna, Leccino,
Maurino, Moresca
Ascolana tenera, Leccino, Nocellara etnea
Ascolana tenera, Manzanillo
Giarraffa, Itrana, Santa Caterina
Ascolana tenera, Carolea, Manzanillo
Arbequina, Ascolana semitenera, Carolea, Giarraffa,
Koroneiki, Leccino, Manzanillo, Maurino
Kalamon (or self-fruitful)
Gordal, Leccino, Maurino, Nocellara etnea,
Picholine, Santa Caterina
Ascolana tenera, Leccino, Manzanillo, Picholine,
Picual
Ascolana semitenera, Carolea, Leccino, Picholine,
Sorani
Bella di Spagna. Giarraffa, Leccino
Ascolana tenera
Ascolana semitenera, Ascolana tenera, Manzanillo,
Maurino, Moresca
Leccino, Maurino
None
Gordal, Marocaine, Moresca, Picholine
Ascolana tenera, Carolea, Leccino
Giarraffa
Gordal sevillana
Grossa di Spagna
Itrana
Kalamon
Koroneiki
Manzanilla
Maurino
Moresca
Nocellara etnea
Pendolino
Picholine
Picual
Santa Caterina
Sorani
Taggiasca
STAMINATE FLOWERS AND POLLEN

The presence of imperfect or staminate owers in olive grants certain
advantages to olive trees (see Fig. 10.2): (i) they can enhance pollen donation
to fertilize other owers; and (ii) they increase the attractiveness to plant
pollinators. Hence, olive owers can receive enough pollen. Therefore, a
correlation exists between the quantity of pollen released and olive
productivity. Furthermore, staminate owers are not a non-functional
byproduct, but they perform a vital role for olive trees (Cuevas and Polito,
2004). The dry weight of staminate owers is 9% less than that of
hermaphrodite owers. Furthermore, concerning the amount of pollen per
anther and pollen viability, no signicant difference was recorded between
staminate and pistillate owers.
97
Fig. 10.2. (a) Olive inorescence during the period of blooming; the ower parts
(petals, stamens and pistil) are easily distinguishable; (b) olive inorescences with
imperfect owers (male) (cv. Chondrolia Chalkidikis).
LOCATION OF FLOWERS WITHIN THE INFLORESCENCE

AND POLLEN
The distribution of the ower (Lavee et al., 1996) within the inorescence is
correlated with the time of anthesis and the percentage of imperfect owers.
Most hermaphrodite owers are located at the apex and the primary pedicels,
while owers in secondary pedicels are mainly staminate and ower later.
FLOWERING AND POLLINATION

In the olive, full blooming is recorded in Mediterranean areas from late May to
rst days of June. The owering period varies between cultivars (see Fig. 10.3),
and this should be taken into account when selecting the most appropriate
pollinators. The blooming period lasts 56 days in any particular cultivar,
though a little more when the weather is cold. The amount of pollen and its
germination capacity is a function of the cultivar, and ranges from low levels to
4050%. Various factors inuence pollination, such as temperature (Galn
98
Chapter 10
Fig. 10.3. Flowering periods of 37 olive cultivars in the Umbria hills of central Italy
(from Antognossi et al., 1975).
et al., 2001), relative humidity, wind and rain. The temperature of the air
should be < 30C. However, very low temperatures reduce the speed of pollen
tube growth, while high temperatures dry out the stigma and do not allow
pollen germination. Wind is important for dissemination of pollen (Griggs et al.,
1975). However, dry and hot winds destroy pollen grains and nullify pollen
germination and pollen tube growth. Rain during the period of blooming also
has a negative inuence on pollen germination. When pollinizers are planted,
their distance from the main cultivar should not be > 30 m, in order to attain
an adequate fruit-setting percentage.
99
Each ower consists of a small calyx, four petals, two stamens and laments
with large anthers and a green pistil with a short style and a large stigma.
Perfect owers have a large green pistil, while imperfect ones are staminate and
do not have a pistil or their pistil is rudimentary (Brooks, 1948).
Pollination in olives occurs through either self-pollination as in the case
of Koroneiki or cross-pollination (Cuevas and Rallo, 1990; Cuevas et al.,
2001), as in Kalamon, and the cultivars are characterized as either selffruitful (Androulakis and Loupassaki, 1990) or non-self-fruitful (Diaz et al.,
2006b) (see Fig. 10.4). In the case of self-pollination, the pollen of a ower
pollinates the pistil of the same ower. The pollen is transferred by gravity, bees
or wind. In cross-pollinated cultivars, the pollen is transferred by wind. Olive
pollen is poor in sugars and therefore bees do not commonly visit olive owers.
(a)
(b)
Fig. 10.4. The cultivar Chondrolia Chalkidikis following (a) self-pollination and
(b) cross-pollination.
100
Chapter 10
When pollen reaches the stigma it germinates, producing the pollen tube,
which conveys the male germ cells (sperm) to the female germ cell (egg). The
pollen tube can be stained for observation by microscopy (Cuevas et al.,
1994b). The pollen tube grows for a few days, reaching the egg cell in the
embryo sac. One sperm cell fuses with the egg, producing the zygote which,
after division, produces the embryo, which is destined to become the olive fruit.
Cold weather reduces the speed of pollen tube growth; in this case, therefore, it
reaches the embryosac late, after deterioration of the latter. After pollination
and fertilization the ovary is transformed into fruit. Each ovary has two
cavities, the locules, each one containing two ovules, i.e. four ovules per ovary.
Only one developed ovule is required for seed formation. The presence of welldeveloped embryo sacs in the ovules and ovule longevity are determining
factors in their capacity for fertilization.
FRUIT SET AND FACTORS AFFECTING IT

Many factors determine fruit set, such as: (i) inorescence load and
distribution (Lavee et al., 1999); (ii) size of and number of orets per
inorescence; (iii) percentage of hermaphrodite owers; (iv) ovarian size; and
(v) physiological condition of the ovules and their longevity.
After fertilization, petals and stamens are abscised and the ovary starts to
grow and enlarge (Rapoport and Rallo, 1991). The average fruit set is about
2%. Factors responsible for inadequate fertilization and subsequent fruitlet
drop include: (i) incompatible pollinating cultivars; (ii) lack of pollinator trees;
(iii) pest and disease problems; (iv) strong winds; (v) water stress and nutrient
stress; and (vi) unfavourable climatic conditions (Cuevas et al., 1995). Usually
one fruit is maintained per inorescence (see Fig. 10.5). Sometimes
shotberries, i.e. fruits which are parthenocarpic (lacking seed), are produced.
The reason why they are produced is unknown. Shotberries mature earlier
than normal fruits.
Number of orets per inorescence

The number of orets is affected by cultivar, the position of the inorescence on
the bearing shoot and the environmental and soil conditions, such as N levels
and water stress.
Perfect and pistillate owers

Olive owers are distinguished by two types, hermaphrodite (perfect) and
staminate (imperfect). Perfect owers have both male and female organs, the
101
Fig. 10.5. High percentage of fruit setting after free pollination of Chondrolia
Chalkidikis.
ovary being relatively large and dark green in color. Imperfect owers have
only stamens and a rudimentary pistil and do not set fruits. The formation of
imperfect owers is due to arrested pistil growth as a consequence of water
stress or N deciency during the period of owering bud differentiation. A small
percentage of setting (12%) is sufcient for a good crop.
Ovarian size
The size of the ovary is affected by water and N availability during the period of
owering bud differentiation. Subsequently, through the process of cell division,
expansion and/or differentiation, ovarian size increases 50-fold (Rapoport et al.,
2004) to produce the olive drupe.
Number and quality of ovules

The olive ovary has two carpels, each one containing two ovules. For efcient
fertilization all the ovules should develop completely. However, of the four
ovules contained in an embryo sac, only one is fertilized and the other three
degenerate and shrink. In olives, degeneration of ovules could occur through
various stress conditions, such as water stress and heat stress. Also, the type
of cultivar affects embryo sac degeneration. When olive trees are not well
102
Chapter 10
supplied with N, ovule viability declines rapidly. Nitrogen concentrations in

leaves of < 1.3% markedly reduce ovule longevity.
The olive tree produces an abundant number of owers, but only 12% of
these develop fruits that reach maturity. The level of normal fruit set in most
cases is based on heavy blooming of olive trees during the on year.
Furthermore, in the off year with limited owering, ower quality is good and
fruit set percentage is increased (Cuevas et al., 1994a; Martins et al., 2006;
Rapoport and Martins, 2006).
Temperature and fruit set

Fruit set is completely inhibited at a constant temperature of 30C, which
signicantly reduces pollen germination but does not prevent pollen tube
growth. The most favourable temperature is 25C, at which faster tube growth
and higher fruit set were both observed. Most fruits are formed at the periphery
of the tree, and very few fruits set in the interior of the olive canopy.
Olive trees are tolerant to high temperatures. However, very high
temperatures during owering may create problems. When the cv. Chondrolia
Chalkidikis is exposed to 35C during owering and 37C before fruit set, fruit set
is reduced (Porlingis and Voyiatzis, 1999). The most favourable temperatures for
owering, pollination and fertilization are between 18 and 22C. The effect of
high temperature could be alleviated by the application of paclobutrazol.
Pollination and fruit set in areas of mild winters

Today, olive cultivation is expanded not only in areas with a Mediterranean
climate with mild, rainy winters and long, warm, dry summers, but also in
other places with very different environmental conditions, e.g. Argentina,
Mexico (Malik and Bradford, 2006c). Early in their life cycle, these trees show
problems with fruit set and yield, with plant growth and metabolism being
affected by the very different environmental conditions found in those regions.
Cross-pollination improves fertilization and fruit set under hot environments,
as well as in years of poor ower quality. The need for cross-pollination exists
when temperatures during olive bloom exceed 30C (Rallo, 1997). Another
problem in warm, dry areas is a very short vernalization period (Ayerza and
Sibbett, 2001). This increases the incompatibility of self-pollination for many
cultivars, including Manzanillo, but it does not affect open pollination (Lavee
et al., 2002).
High temperatures during olive owering increase pollen incompatibility.
Pollen tubes of the same cultivar frequently become blocked between the
stigma and embryo sac in this condition. Furthermore, under hot, arid
conditions the distance for effective pollination is reduced and is < 30 m radius,
103
which is at variance with normal conditions. Increasing the number of

pollinator trees in a grove could solve some of these problems, although it
introduces the problem of establishing monocultivar plantings.
FRUIT GROWTH
The olive fruit is a drupe consisting of the pericarp and endocarp. The pericarp
consists of the skin (exocarp) and the esh (mesocarp), the latter producing the
oil. The endocarp consists of a lignied shell which encloses the seed; each seed
consists of the seed coat and the endosperm. The seed also contains an embryo,
two cotyledons, a radicle and a plumule. The shape and size of the olive fruit
depends on the particular cultivar: there are small-, medium- and large-fruited
cultivars.
Olive fruit development follows a double sigmoid curve, very common in
other fruits, having three stages. In the rst stage, growth is fast; the growth is
exponential and is characterized by cell division, and the pit is still growing. In
the second stage the growth slows down or stops. However, at this stage the pit
hardens and attains its nal size. Finally, in the third stage the fruit grows by
cell enlargement.
FURTHER READING
Acebedo, M.M., Caete, M.L. and Cuevas, J. (2000) Processes affecting fruit distribution
and its quality in the canopy of olive trees. Advances in Horticultural Science 14(4),
169175.
Batanero, E., Ledesma, A., Villalba, M. and Rondriguez, R. (1997) Purication, amino
acid sequence and characterization of Ole e 6, a cystein-enriched allergen from
olive tree pollen. FEBS Letters 410, 293296.
Bernier, G., Kinet, J.M. and Sachs, R. (1981) The Physiology of Flowering. Volume II.
Transition to Reproductive Growth. CRC Press, Boca Raton, Florida, pp. 157159.
Chaari-Rkhis, A., Maalej, M., Messaoud, S.O. and Drira, N. (2006) In vitro vegetative
growth and owering of olive tree in response to GA3 treatment. African Journal of
Biotechnology 5(22), 20972302.
Cimato, A., Cantini, C. and Sillari, B. (1990) A method of pruning for the recovery of
olive productivity. Acta Horticulturae 286, 251254.
Cuevas, J. and Polito, V.S. (1997) Compatibility relationships in Manzanillo olive.
Journal of Horticultural Science 32, 10561058.
Fernndez-Escobar, R., Sanchez-Zamora, M.A., Uceda, M. and Beltran, G. (2002) The
effect of nitrogen overfertilization on olive tree growth and oil quality. Acta Journal
of Horticulturae 586, 429431.
Fernndez-Escobar, R., Benlloch, M., Herrera, E. and Garcia-Novelo, J.M. (2004) Effect
of traditional and slow-release N fertilizers on growth of olive nursery plants and N
losses by leaching. Scientia Horticulturae 101, 3949.
104
Chapter 10
Gioulekas, D., Chatzigeorgiou, G., Lykogiannis, S., Papakosta, D., Mpalafoutis, C. and
Spieksma, F.T.M. (1991) Olea europaea 3-year pollen record in the area of
Thessaloniki, Greece, and its sensitizing signicance. Aerobiologia 7, 5761.
Kitsaki, C.K., Drossopoulos, J.B. and Terzis, S. (1995) Endogenous free abscisic acid in
oral, bark and leaf tissues of olive during anthesis and early fruit development.
Scientia Horticulturae 64, 95102.
Marquez, J.A., Benlloch, M. and Rallo, L. (1990) Seasonal changes of glucose,
potassium and rubidium in Gordal Sevillana olive in relation to fruitfulness. Acta
Horticulturae 286, 191194.
Martin, G., Nishijima, C. and Early, J. (1993) Sources of variation in olive ower and
fruit populations. HortScience 28, 697698.
Navarro, C., Benlloch, M. and Fernndez-Escobar, R. (1990) Biochemical and
morphological changes related to owering in the olive. In: XXIII International
Horticulture Congress, Florence, Italy, Abstract 3196.
Stutte, G.W. and Martin, G.C. (1986) Effect of light and carbohydrate reserves on
owering in olive. Journal of the American Society of Horticultural Science 111,
2731.
Vitagliano, C., Minocci, A., Sebastiani, L., Panicucci, A. and Lorenzini, G. (1999)
Physiological response of two olive genotypes to gaseous pollutants. Acta
11
ALTERNATE BEARING
INTRODUCTION
The productivity of each cultivar is a function of alternate-bearing habit and fruit
abscission before ripening. Alternate bearing is dened as the characteristic of an
olive tree to produce olives every 2 or more years. The year of maximum
production is called the on year, while the year with minimum or no production
is called the off year.
In olive trees we observe two growth ushes, the rst one in spring and the
second in autumn (October), under adequate soil moisture and temperature.
Alternate bearing is directed by various factors such as water stress, warm soil,
climatic factors and the cultivar. Signicant genotypic variation was reported
concerning cultivars, with respect to alternate bearing. The main causal factor
of alternate bearing is the inhibition of ower bud induction by the seed of
growing fruits, and this is due to competition for nutrients.
The two main factors that can reduce the intensity of alternate bearing are
pruning and productivity. Furthermore, the irrigation schedule may reduce
alternate bearing. Mineral nutrition is also a determining factor. Nutrient
deciency, especially of N during the period of ower bud differentiation, affects
vegetative vigour, the size of fruits and the owering of the following spring
(Fernndez-Escobar et al., 1999, 2004b).
THE EFFECT OF FRUIT LOAD

The inhibition of oral induction by seeded developing fruits is the major factor
in the olives biennial behaviour. Environmental and other factors such as
cultivation practices that affect aspects other than oral induction may
amplify the alternate-bearing habit. Such factors include irrigation, pruning,
fertilization, etc. Therefore, the control of biennial bearing should be achieved
by (i) selection for breeding cultivars that produce every year; and (ii) cultural
practices to stabilize the oral induction percentage.
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106
Chapter 11
GIRDLING
Many horticultural techniques such as girdling can increase ower initiation
and fruit set in the off years (Hartmann, 1950; Lavee et al., 1983; Ben-Tal and
Lavee, 1984; Li et al., 2003). Girdling increases soluble sugar and starch
content in leaves and shoot bark above the girdle during the off year. Trees
during the on year do not accumulate carbohydrates above the girdle.
Furthermore, girdling reduces soluble sugar and starch concentrations below
the girdle in both on and off trees. Girdling affects carbohydrate-related gene
expression (Weiss and Goldschmidt, 2003).
ENDOGENOUS HORMONES
Endogenous plant hormones play a signicant role in alternate bearing (Lavee,
1989; lger et al., 1999). ABA and GA3 have a direct role in ower bud
initiation, while IAA and IAA-like compounds have an indirect role in the
initiation of ower buds. Furthermore, exogenous application of growth
regulators (Ben-Tal and Lavee, 1976; Akilioglu, 1991; Eris and Barut, 1991),
spermine or spermidine (Pritsa and Voyiatzis, 2005) or paclobutrazol (Lavee
and Haskal, 1993) may alleviate the problem.
PROTEIN CONTENT
The total extractable protein content in the leaves of off trees is signicantly
lower than in the leaves of on trees (Lavee and Avidan, 1994). This was
observed in the cultivars Koroneiki, Uovo de piccione, Manzanillo and
Barnea. The least difference was recorded in the leaves of Koroneiki, which
demonstrates the least alternating capacity.
Chlorogenic acid (CHA) is associated with alternate bearing and levels are
high in the alternate years and low in the off years. Injection of exogenous CHA
reduces ower bud differentiation when applied during the secondary
induction period in the autumn and early winter.
In most cases proteins of 28 and 32 kD were present in samples of the off
year leaves, and 22 and 14 kD were in higher concentration in the leaves of the
on trees.
PHENOLIC ACIDS
Endogenous control of alternate-bearing includes the possible involvement of
phenolic acids, with the end product of caffeoylquinic acid (see Fig. 11.1)
(Lavee and Avidan, 1982; Lavee et al., 1986).
Alternate Bearing
107
Shikimic acid
Phenylalanine (Tyrosine)
Cinnamylquinic acid
p-Coumarylquinic acid
5-Caffeoylquinic acid
Cinnamic acid
p-Coumaric acid
Caffeic acid
Fig. 11.1. Pathway for the production of 5-caffeoylquinic acid via cinnamylquinic
acid (from Lavee et al., 1986).
FURTHER READING
Martin, G.C., Ferguson, L. and Polito, V.S. (1994) Flowering, pollination, fruiting,
alternate bearing and abscission. In: Ferguson, L., Sibbett, G.S. and Martin, G.C.
(eds) Olive Production Manual. University of California, Division of Agriculture and
Natural Resources, Berkeley, California, Publication 3353, 1921.
Rallo, L., Martin, G.C. and Lavee, S. (1981) Relationship between abnormal embryo sac
development and fruitfulness in olive. Journal of the American Society of Horticultural
Science 106, 813817.
Rallo, L., Torreo, P., Vargas, A. and Alvarado, J. (1994) Dormancy and alternate
bearing in olive. Acta Horticulturae 356, 127136.
12
FRUIT THINNING
INTRODUCTION
Fruit size is the determining factor in the price offered per kg of raw table olives
to growers. Therefore, the study of factors that tend to increase fruit size is
fundamental. The rst fruit drop takes place after petal fall. During this
process a heavy shedding of undifferentiated or poorly developed olives is
recorded due to fruit competition (Surez et al., 1984). After fruit set the olive
fruit is small, and various factors can inuence its nal size.
The growth of olive fruit is performed initially by cell division and, later, by
cell enlargement. Together with cell enlargement, air spaces increase to a
maximum. The combination of cell division, cell enlargement and air space
appearance results in the olive growth curve. The olive fruit size, especially in
table olives, is increased by the following factors:
Adequate soil moisture.

Excess N fertilization.
High leaf:fruit ratio.
Light owering and fruit set.
Appropriate pruning.
Early thinning, stimulating cell division.
OBJECTIVES OF OLIVE FRUIT THINNING

Olive fruit thinning aims at reducing limb breakage, increasing fruit size,
improving fruit quality and stimulating ower bud initiation for the following
years production.
109
110
Chapter 12
LEAF:FRUIT RATIO AND ASSIMILATE TRANSPORT

Thinning modies the leaf:fruit ratio; therefore, it reduces fruit competition in olive
(Rallo and Fernndez-Escobar, 1985). A change in the leaf:fruit ratio causes
signicant differences in fruit growth. Under high levels of assimilate availability in
fruits, as happens following fruit thinning, the fruit weight increases. In contrast,
leaf removal reduces olive fruit size. Growth and fruit size are independent of each
other, and neighbouring shoots can be considered an independent unit, since this
will not be affected by conditions occurring in adjacent shoots. (Proietti and
Tombesi, 1996b). The nutrients for olive fruit growth and development are
supplied mainly by the leaves of the same shoot. Therefore, in shaded areas where
carbohydrate availability is low, olive fruits cannot obtain optimum size although
the availability for the whole tree may be high. Fruit thinning increases
carbohydrate availability to remaining fruits and results in an increase in size. The
factors, which increase or decrease thinning, are listed in Table 12.1.
THINNING METHODS
Generally, olive thinning can be carried out using the following methods:
hand-, mechanical and chemical thinning.
Hand-thinning
Hand-thinning is the removal of owers or fruits manually. Our method is to
leave six to seven fruits per shoot (30 cm). This method incurs a heavy labour
Table 12.1. Factors which increase or decrease fruit thinning (from Westwood, 1978).
Increased thinning
Decreased thinning
Young trees
Moist weather
High relative humidity
High maximum temperature
Softly sprayed water
Slow drying conditions
High concentration of chemicals
Trees of low vigour
Close spacing of trees
Light pruning
Heavy owering
Poor pollination
Previous heavy crop
Addition of wetting agents
Mature trees
Dry weather
Low relative humidity
Temperature lower than maximum
Heavily sprayed water
Fast drying conditions
Low concentration of chemicals
Vigorous trees
Wide planting distances
Severe pruning
Light owering percentage
Adequate pollination
Previous light crop
No inclusion of wetting agents
Fruit Thinning
111
cost. Thinning should be conducted within 3 weeks of full bloom and no later,
otherwise there is no reduction in alternate bearing and fruit size will not be
substantially increased.
As an alternative to hand-thinning, appropriate pruning can thin the
shoots, reduce cropping and increase olive fruit size. Furthermore, this promotes
shoot growth for the following years crop.
Mechanical thinning
Mechanical thinning can be performed by the following methods:
1. Use of a hand-operated spray, with high-pressure water (Westwood, 1978).
2. Use of a mechanical shaker to shake the tree trunk. This method needs skill
to avoid over-thinning. Furthermore, with this method the larger olives are
removed since these fruits obtain a greater directional momentum than small
ones. Also, fruits from the stronger branches are removed more easily.
Therefore, with mechanical thinning it is difcult to achieve the degree of
thinning the grower needs.
Chemical thinning
In recent decades, NAA, l-naphthaleneacetamide (NAAm) and ethephonreleasing ethylene have been tested for thinning table olives (Weis et al., 1988,
1991). Chemical thinning has certain advantages over hand- or mechanical
thinning:
Low thinning cost.

Greater size of olives, earlier maturation and better quality.
Decrease in biennial bearing.
The disadvantages of this method include the following. Sometimes the

results are variable and depend on various factors that may cause overthinning. These factors include: (i) age of the tree; (ii) very low-vigour trees; (iii)
light pruning; (iv) heavy bloom; (v) poor pollination; (vi) high humidity or high
temperature; and (vii) high concentration of thinning agents. The lack of stable
response is a common problem due to thinning (Westwood, 1978).
However, thinning of table olives produces certain benets such as:
1. Large fruit size. This is a critical factor for the price per kg paid by the
processor to growers. Trees overloaded give small fruits and enter into biennial
bearing. Thinning increases the ratio of leaves per fruit, i.e. greater assimilate
amounts are supplied to each fruit and the fruit obtains a greater size.
2. Annual bearing and precocious fruit ripening. A heavy crop of olives (on year)
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Chapter 12
is followed by a light crop (off year) or no crop at all. However, an average crop
achieved with fruit thinning gives a satisfactory crop the following year. Therefore,
fruit thinning results in bearing every year. Furthermore, after thinning, the crop
is moderate, has more available carbohydrates per fruit and matures earlier.
3. Flesh:pit ratio and olive fruit quality. Thinned trees give fruits with a higher
esh:pit ratio and higher oil content. The harvesting cost per kg is signicantly
less from trees with moderate yield and large fruits than from trees with a big
fruit load.
4. Flower bud differentiation. Flower bud differentiation increases as leaf
assimilates and other accumulated reserve materials increase.
For the chemical thinning of olive, NAA is used. The time of application is
very important in the effectiveness of thinning (Lavee and Spiegel-Roy, 1967).
The best time for thinning is 1218 days following full bloom. If NAA is sprayed
earlier, this treatment over-thins the olive tree, while late application is not
effective. The trees should be fully covered with the spray solution, the required
quantity being 1015 l/tree. The suggested NAA concentration is 10 ppm/day
after full bloom, i.e. 100 ppm for spraying after 10 days or 150 ppm for spraying
15 days after full bloom.
The diameter of table olives should be 35 mm at the time of application.
Furthermore, the greater the applied concentration of NAA the greater the
thinning activity (Sibbett and Martin, 1981; Maranto and Krueger, 1994).
13
SYSTEMS OF PLANTING AND
CANOPY TRAINING
SELECTION OF THE ORCHARD SITE

Since the olive tree grows and is more productive in heavy and fertile soils and
less so in poor/dry ones, for the expansion of new olive plantings, fertile soils
are preferred (Sibbett and Osgood, 1994). This does not mean that the modern
olive culture should be expanded only to richer soils, since other crops in such
soils may be more efcient than olives. Concerning the climatic conditions of
the area for olive cultivation, these should be favourable for the growth and
productivity of the trees. Furthermore, it is important that the selected area be
irrigated and mechanically cultivated.
SOIL PREPARATION
Under favourable conditions the root system of olive trees, planted at distances
of 6 6 m, covers within a period of 34 years the entire area of the orchard.
Soil preparation includes levelling, deep ploughing, drainage, etc. and should
be performed before planting (Sibbett and Osgood, 1994). Large stones should
be removed to avoid damage to agricultural machinery.
SELECTION OF CULTIVAR AND ROOTSTOCK

Local experience plays a signicant role in the selection of the best suited
cultivars within a particular area. Furthermore, the new cultivation techniques
of intense planting incorporate certain modications of the traditional methods,
and this affects the choice of cultivar. For example, large-fruited cultivars that
are suitable for table olives and olive oil production are preferred, due to low cost
and easiness of harvesting.
The planting material is either budded plants or plants obtained asexually
from leafy cuttings. In the traditional olive groves budded plants on Olea sativa
113
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Chapter 13
(wild olive) are preferred, while in the dense planting systems, plants from leafy
cuttings are the rule. However, until now little progress has been conducted in
the direction of olive rootstocks, since the rootstocks used are seedlings from
wild olives or cultivated varieties, giving plants with various characteristics and
properties. The genera and species tested as olive rootstocks are the following:
Phyllirea, Ligustrum, Syringa, Chionanthus, Forsythia, Fraxinus forestiera, Olea
verrucosa, O. chrysophylla and O. oblonga. These rootstocks do not have good
afnity with O. europaea. Furthermore, rooted cuttings from low-vigour
cultivars could be used as dwarf rootstocks. The root system of rooted cuttings
differs from that of grafted plants. Rooted cuttings have all their roots at the
same level (base of cuttings) and tend to grow horizontally and close to the soil
surface. In grafted plants, by contrast, the roots appear along an axis of 40 cm
and grow at a greater depth. These differences may disappear after a few years,
but they play an important role during the rst few years of growth in the
plants tolerance to drought and frost. The planting material should be healthy
and have adequate branching along the central axis.
SYSTEMS AND DISTANCES FOR PLANTING

The planting distances and the number of trees/1000 m2 of olive grove are
presented in Table 13.1. Before establishing an olive orchard it is very
important to study the socio-economic conditions of the area. These
conditions change with time and their trend should be determined. Local
conditions include the examination of soil, climatic factors and cultivar
characteristics. If the area under cultivation is covered with bushes, these
should be removed and the area cultivated with annual crops for 23 years
before planting. This decreases the danger from Armillaria and other soil fungi
growing on roots. Levelling of the area follows and the irrigation system is
established.
A planting, to be laid out in a regular pattern (square, rectangle or
triangle), is started by establishing a straight base line, which is usually
adjacent to a fence or roadway. Then, lines at right angles to the baseline are
established at both ends and at one or two points in the middle of the plot. Right
angles are usually established by using three ropes whose lengths are in the
proportion 3:4:5 (e.g. 30, 40, 50 m). The 40 m rope is set along the baseline,
then the 30 m one at 90 and nally lay the 50 m rope to close the triangle. In
this way we can establish the positions of all trees. Small stakes can be used to
mark the locations for tree holes.
When the slope of the area is greater than 3%, a contour layout is used. The
rst row is at the highest elevation and is staked out with all the points at the
same elevation. Next, the steepest slope along this rst row is found and the
distance which was selected as the minimum distance between rows is
measured down the slope. Continuing in the same way, the rows are established.
Systems of Planting and Canopy Training
115
Table 13.1. Planting and spacing distances in the orchard.

Planting
distance (m)
Area/tree
(m2)
Trees/1000 m2
33
34
35
36
44
45
46
47
48
55
56
57
58
66
67
68
77
78
79
88
89
8 10
99
9 10
10 10
9
12
15
18
16
20
24
28
32
25
30
35
40
36
42
48
49
56
63
64
72
80
81
90
100
111
83
67
55.5
62.5
50
41.5
35.5
31
40
33
28.5
25
27.5
24
21
20.5
18
16
15.5
14
12.5
12
11
10
Spacing at full
production (m)
66
64
65
66
48
85
For irrigated orchards

The planting of olive trees is conducted from November to February. In

areas with mild winters the best period for planting is the autumn, while in
those areas with cold winters planting is postponed until the end of winter.
PLANTING
For planting, trees in 3 l black plastic bags are used. Before planting, these bags
are removed. The depth of planting is a little greater that in the nursery. The
main object in planting is to protect the roots from drying or freezing and to get
them into rm contact with moist soil. Tree holes should be large enough to
accommodate the root system a hole of depth 80100 cm is adequate. Long
roots can be cut back enough to balance the root system. Topsoil should be put
around the roots and rmed in with the foot after the hole is about half-full of
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Chapter 13
soil. The hole should then be lled the rest of the way up with loose soil. Weeds,
manure and other organic materials should be kept out of the hole at planting
time to avoid damage to the root system. In poor soils, a surface application of
N fertilizer is recommended.
The most serious mistakes made during the establishment of an olive grove
are improper irrigation and lack of weed control, which result in slow growth
rate and tree death within the rst few years of planting. During the early years
of orchard life irrigation water should be applied many times, but the quantity
of the water at each irrigation should be low to medium.
In traditional olive orchards planted on steep land, distances between trees
should be long; short planting distances in muddy soils produce high trees with
low leaf mass and very low productivity. As a general rule for the distances of
planting, the distances between two trees should be double the radius of the top
growth.
Planting distances are a function of the soil fertility, cultivar and planting
system. The ratio of main cultivar:pollinizer may be 1:1, 2:1, 4:1, 8:1, 10:1 or
18.5:1 (see Fig. 13.1). The most common shape for the olive tree is the open
centre tree. This shape allows light interception for the inner part of the top and
increases tree productivity. The distance of planting with this system in areas of
adequate precipitation is 10 10 m, in order to avoid tree crowding. However,
when precipitation is not adequate and the soil is poor, greater distances of
planting are preferable. Olive plants after planting are not pruned for 36
years, to speed up the onset of fruiting and to allow branches to develop at
intervals along the trunk. Subsequently, three to ve branches are selected
and, if the central axis is not pruned, the top of the tree attains the form of a
sphere (spherical shape).
PLANTATION SYSTEMS
Four major types of olive plantation systems are identied: traditional, semiintensive, intensive and organic (Metzidakis and Koubouris, 2006). The
classication of olive production systems on the basis of agro-ecological,
technological and socio-economic criteria is analysed below.
1. Traditional. This is characterized by low-density plantations (< 100
trees/ha) of old trees (> 50 years) planted on moderately steep slopes. This
system has low inputs, no mechanization and results in low yields not
exceeding 10002000 kg/ha and low protability.
2. Semi-intensive. The density in this system is variable, uctuating between
100 and 150 trees/ha. These orchards have productive trees, yielding, for
example, 28004000 kg/ha in Crete.
3. Intensive. Tree density is 250 trees/ha, yielding up to 5000 kg/ha and the
system may be mechanized.
117
Fig. 13.1. Diagram of planting of two olive cultivars at ratios 1:1 (a, b), 2:1 (c), 4:1
(d), 8:1 (e), 10:1 (f) and 18.5:1 (g).
, main cultivar; pollinizer.
4. Organic. These orchards do not have a mathematical form and can be

established following mechanization of orchard management. This system is
labour-intensive but it produces high-quality olive oil. Organic olive orchards
in Greece represent 1% of total area planted.
MODERN TRAINING SYSTEMS

Vase system
This is the most common system in many areas of the world. Olive trees in the
vase form have greater photosynthetic surface per tree in comparison with those
trained in the globe system. The vase form includes the cone, inverted cone, the
cylinder or multiple cones (Garcia-Ortiz et al., 1997; Gucci and Cantini, 2004b).
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Chapter 13
Each tree has a single trunk and three to ve branches forming an angle of
45. The height of the trunk is 0.30.4 m for hand-harvested trees or
1.01.2 m for orchards adapted to mechanical harvesting. In mechanically
harvested orchards the branches are more erect.
Polyconic system
This represents a modication of the vase form. Trees trained to a polyconic
vase form have a single trunk (0.81.6 m) and three to six major branches, of
varying orientation, in order to achieve optimum light and space utilization
(Cain, 1972; Jackson, 1980; Connor, 2006). Each branch is trained to a cone.
This system is widespread in Italy, although today its expansion is not
recommended, since it requires high labour input for pruning and harvesting.
Central leader trees or monocone system

This is an alternative form of the polyconic vase and is recommended for highdensity plantings, and is very common in Italy (see Fig. 13.2). The tree has a
trunk up to 1.01.2 m in height; the primary branches have an elicoidal
arrangement along the central axis. The length of lateral branches decreases
Fig. 13.2. Plantation of olive trees in double rows (6 3 m) in the monocone system.
119
from the base to the top of the tree and the tree becomes conical in shape. The
main advantage of this system is that it is appropriate for mechanical
harvesting with trunk shakers (Preziosi et al., 1994). However, it incurs a high
pruning cost since the trees become tall. Trees of the Kalamon cultivar trained
in the monocone system and within a controlled irrigation regime produce
good-quality olive oil (Pallioti et al., 1999; Patumi et al., 2002).
Vasebush system
The vasebush system has a vase form and the trunk and primary branches
originate from the crown of the tree or from a very short trunk. Each plant has
three to six branches. The height of the trees is relatively small, appropriate for
hand-harvesting, and especially for table olives (Fig. 13.3). This system is not
good for mechanical harvesting.
Planting of olive orchards in this form started in the 1950s/60s and the
system continues to be expanded due to its following advantages:
Rapid progress to fruit bearing (34 years).

Triplicate yield/unit area in comparison with the free vase form planted at
distances of 10 10 m.
Reduction of harvesting cost by 70%.
Ease of cultivation.
Fig. 13.3. Olive planting (cv. Chondrolia Chalkidikis) in high-density orchards

and top pruning in dwarf bushes (vase form).
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Chapter 13
The planting distances are 57 m within rows and 57 m between rows.

Smaller planting distances lead to shading after 10 years of growth. Greater
planting distances entail inefcient use of the soil for the rst 10 years.
After 1015 years have elapsed since planting, rejuvenation of the
treetops is necessary. In order to shape the tree into a bush vase, planting
material is derived from leafy cuttings and the plants have many side-shoots.
The trunk of the vasebush is short (< 50 cm), and from this grow three to four
branches at spacings of 1015 cm. For medium-vigour cultivars this small
number of branches is adequate. Along the main branches, secondary
branches develop at spacings of 50 cm, and their total number is six, eight, 12
or 16 depending on tree vigour.
The high-density system of olive production is efcient, and reliable
annual yields of about 34 t/ha can be obtained in irrigated orchards with
2040 trees/1000 m2. These trees attain full productivity in about 8 years,
and the orchards yield is considerable. Harvesting is achieved manually, with
trunk shakers or by over-the-row, comb-type harvesters, which are very
efcient in fruit removal.
The failings of this system include the following:
Long time (8 years) to achieve full production.

Harvesting costs with trunk shakers are high and exceed 50% of the total
production costs.
Spray coverage and pest control are less efcient in comparison with those
for smaller trees.
A high-density orchard also requires very precise irrigation, good fertility
management and sometimes more careful pest control and greater
investment costs.
Palmette system
This system is an efcient method for other fruit trees such as pear and apple,
and has been tested in olive culture. It incurs high costs for planting, pruning
and maintenance.
Globe system
This system utilizes single-trunk trees and the shape of the canopy is round,
ellipsoidal or hemispherical. The canopy is not open, unlike the vase form, and
is occupied by secondary branches. Light penetration in this system is
inadequate and yields are generally poor (Gucci and Cantini, 2004b). At
planting the central axis is left alone and its terminal parts ll the centre of the
canopy. To avoid excessive tree height the primary branches are shortened. The
globe system is appropriate for traditional, non-irrigated orchards.
121
High-density system
High density is a recent olive-growing technique, and many workers have
reported their experiences with it (Fontanazza and Cappelletti, 1993; Dag et al.,
2006; Tous et al., 2006). The main features are listed below:
1. Increase in global food demands. The world population increase and the
improvement in living standards has led to increased food consumption.
Increase in total yield is achieved by both increasing the cultivated area and
increasing the yield per unit of area, which is feasible with high-density systems.
2. Lack of labour. Technological, nancial and social progress have, as a
consequence, forced many rural manual workers to move to the cities for better
conditions. The consequence of this is that the number of people working in
olive groves is now signicantly reduced.
3. Lifespan of the olive tree. Social and economical conditions change very
rapidly, as do the preferences of consumers. For this reason the lifespan of olive
trees today does not have the signicance it previously had.
4. The time required for the olive trees to bear fruit. It is now more important
to reduce the required time necessary for olive trees to start bearing than to
achieve long-lived trees. In the traditional olive orchards full fruit bearing was
achieved within 78 years of planting. This long period does not encourage
olive planting in fertile soils where other crops might be more productive
densely planted orchards start producing within 34 years. In traditional olive
orchards, parents plant olive trees for the benet of their children, while in the
modern, high-density systems orchards are planted for the benet of the
present generation and less so for the following.
5. Density of olive planting. The area of the orchard covered by the tree
canopy depends on the tree age, shape of tree and the tree number/unit area.
In traditional olive orchards many years are required for achievement of the
maximum coverage of the area by the treetops. In high-density planting soil
coverage is rapid, leading to optimum use of the soil.
6. Volume of the treetops. During orchard establishment the tree
arrangement and the distance of planting are based on the maximum
dimensions that the canopy allows. A smaller treetop volume secures denser
planting. The productivity per unit area of the soil covered by the tops is greater
in densely planted orchards. Hence, the correlation between the yield per unit
soil area covered and the dimension of the tops is negative.
The establishment of dense plantings does not differ from the planting of
classical olive orchards concerning the techniques used and soil preparation.
In the case of dense planting intercropping is avoided. However, in the case of
intercropping the following parameters should be taken into account:
The differential requirements of olive and other crops concerning soil

preparation.
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Chapter 13
Fertilizers applied (type, quantity, time of application).

Compatibility of insecticides and fungicides for olives and secondary crops.
APPROPRIATE VARIETIES
When selecting cultivars the best ones for a high-density system are the lowvigour varieties with compact growth habit, early bearing and fructication in
clusters. Such cultivars are the clone Arbequina IRTA-118, Arbosana-643,
Ioanenca, Canetera, FS-17, Urano, Askal and Koroneiki. Of those
varieties the three most appropriate for super-high-density systems are
Arbequina, Arbosana and Koroneiki (Tous et al., 2004). These varieties grow
upright (compact), have a central leader and very few lateral branches, are selfcompatible, very precocious and produce olive oils of excellent quality. Due to
heavy crop load and the annual bearing habit their size is medium. Among
these three varieties Arbequina is the most frequently planted variety for highand super-high-density plantings in Spain. Arbequina is considered frost hardy
and well adapted to different soil types. It withstands temperatures down to
6C (22F) with no apparent problems. The fruit has an oil content of 2022%
and is quite aromatic and fruity, with very little pungency or bitterness and a
short shelf life of about 1 year. The tree is relatively small, with low vigour and
can be shaped and kept small by pruning. The inorescences are long, with
2227 owers. The fruit is small (1.51.8 g) and round in shape. At maturation
the fruit is uniformly dark black and the stone is large (1820%).
The second recommended variety is Arbosana, which matures 3 weeks
later and has 25% less vigour than Arbequina, is more sensitive to water stress
and presents more vigourous regrowth after pruning. The oil of Arbosana is
more bitter to the taste than that of Arbequina, with a fruity and pleasant
avour. This cultivar is characterized by high productivity and resistance to low
temperatures, leaf drop and olive knot (Pseudomonas savastanoi). The clone is
propagated asexually and it produces 1920% olive oil. Its leaves are small in size,
5870 mm in length and 1112 mm in width. The colour of the upper surface is
green and that of the lower surface chrysolite green. The petiole is short, 5 mm.
The fruit is early in maturation; it matures by the fourth week of October and is
elliptical in shape. Composition of the oil is: palmitic acid (C 16:0), 1.3%; esteric
acid (C 18:0), 2.0%; oleic acid (C 18:1), 74%; linoleic acid (C 18:2), 7.66%;
polyphenols K225 (bitterness), 0.24%. The stability of this oil is 13.5 h at 120C.
The third variety is the Greek cultivar Koroneiki, with a high percentage
of fruit set and very small fruit. The oil from Koroneiki is green in colour, very
fruity, medium-bitter and pungent. Its shelf life is > 2 years.
With regard to the other varieties, Canetera is more sensitive to Verticillium
dahliae than is Koroneiki. Arbosana is sensitive to peacock spot, and FS-17 to
Colletotrichum gloeosporioides.
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SUPER-HIGH-DENSITY ORCHARDS
In the early 1990s, a new densely planted olive orchard appeared in Spain,
which was later introduced to other countries, such as Portugal, Morocco,
Tunisia, France, Chile, Argentina, California and Greece. Today, globally, there
are about 30,000 ha planted to this system, with more than 60% being in
Spain. This system allows mechanization of harvesting and signicant cost
reduction. This is accomplished by using continuous harvesting machines,
thus reducing the harvesting cost by up to 50% in comparison with the system
using trunk shakers. However, the drawbacks of this system are the high
planting cost (many plants), the need for larger-scale orchards, the need for
cultivars with medium to low vigour, the many problems of pests and diseases
like Cycloconium, the need for irrigation and the capital required for the
harvesting machines. To avoid problems with Cycloconium, drier soils are
suggested, with wider tree spacings and application of copper fungicide sprays.
Tree spacing is about 1.32.0 m and about 3.04.3 m between rows.
Modern plantations use greater tree spacing, i.e. 1.52.0 m 4.04.5m
(190220 trees/1000 m2) and rows are oriented eastwest. After 34 years a
hedgerow is formed and the system requires a central leader (monocone), with
one stake per tree. The super-high-density system requires a trellis system or at
least a stake for each tree (Iannotta and Perri, 2006; Vossen, 2006); most
orchards use a single wire for support. Young trees should be tied to the stake
about every 3040 cm and remain unpruned the rst 2 years. At the end of
the second year, the lower (1 m) branches and any suckers should be removed
to avoid interference with the central leader. After the rst harvest (third year)
the large lateral branches (> 2 cm diameter) are cut out; this allows adequate
light interception and penetration into the treetops. Also, in the summer of the
fourth year the trees should be topped to a height of 3.03.3 m; this reduces
the shading of the lower branches and adjacent rows. From the fourth and
subsequent years the trees are pruned by hand to maintain the lower part
(0.61.0 m) clean (no branches), in order to achieve good harvester frame
closure around the trunk. In order to reduce vigour the application of nitrogen
is reduced after the fourth year. At full productivity (78 years old) the
recommended amount of N applied is 5 kg/1000 m2. With regard to P and K,
standard rates are applied. The amount of water applied by drip irrigation is
about 200 m3/1000 m2. Controlled-decit irrigation is utilized after the fourth
year to reduce vigour and improve olive oil quality.
YIELD PER UNIT AREA

The average production is 1.9 t/ha in the third year, 2.5 t/ha in the fourth year,
2.7 t/ha in the fth year and 3.3 t/ha in the sixth year after planting. In
orchards 58 years old the yield ranged from 1.2 to 78 t/ha depending on
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Chapter 13
light penetration. The likely yield overall is around 4 t/ha, which is considered
adequate if we take into account that the orchard does not have alternate
bearing and the cost of harvesting is very low.
MECHANICAL HARVESTING BY OVER-THE-ROW HARVESTER

For mechanical harvesting, the over-the-row modied grape harvester is now
used (Tombesi, 2006). This machine requires two people, one operator driving
the harvester and another transferring the fruit in the eld to the mill for
processing. The capacity of a straddle harvester is 2 ha/h. The cost of
mechanical harvesting comprises only 20% of the total production cost. Various
types of harvesters are used, the most common being the Gregoire brand, which
has beating bars that strike the tree from both sides, and collects the olives onto
a moving conveyor belt that transports them to bins emptied periodically.
Damage to olive trees by this machine is minimal. Other harvesting machines
include the Korvan, Agright, Braud, New Holland and Pellenc.
14
PROPAGATION OF OLIVE TREES
INTRODUCTION
The olive tree, until the second half of the 19th century, had been propagated
only asexually by using large cuttings, ovules or rooted suckers. Later, towards
the end of that century, indirect methods of multiplication such as graftingon seedlings were preferred in order to face the increasing demand for
specimens for new plantings. If we consider the published papers over recent
decades, we nd that the most studied technique by far has been propagation
by cuttings. Furthermore, interest in grafting and propagation by seed
continues. Therefore, it is worthwhile studying all aspects of olive propagation,
from traditional systems to in vitro propagation and other more advanced
biotechnologies (Fabbri, 2006).
PROPAGATION BY SEED
Olives can be propagated very easily. The morphology of olive stones of nine
olive cultivars is given in Fig. 14.1. There are a number of ways of propagating
olive plants (Hartmann et al., 2001; Fabbri et al., 2004). Olive plants may be
grown from seed, but a true cultivar will not be obtained from seed
propagation. Olive seedlings are sometimes used as rootstocks to which some
known cultivars are grafted. Seeds are cracked or treated with sulphuric acid
as an aid to germination because the pits are very hard. Seed propagation aims
at producing seedlings necessary for rootstocks. It is very simple and requires
neither specic equipment nor experience. However, propagation by seed
produces seedlings and grafted plants variable in vigour and size. Seedlings
start fruiting after 1215 years and they have a deep root system, while olive
trees from asexual propagation have a shallow root system and start fruiting
after only 35 years.
125
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Chapter 14
Fig. 14.1. Olive stones from nine cultivars after separation from the fruit pulp and
cleaning.
Seed collection, seed storage and breaking dormancy

For seed collection, the best sources are trees of certain cultivars, selected for
their good characteristics and regular and high productivity. Selected trees
should originate from a monocultivar orchard, in order to avoid crosspollination that might lead to variability in seedling quality. These trees should
be marked as being suitable for seed collection for several years. Cultivars used
for seed collection include Arbequina in Spain, Frantoio in Australia,
Oblonga and Allegra in the USA, Canino, Mignolo, Frangivento and
Propagation of Olive Trees
127
Maurino in Italy and Olea sylvestris, Chondrolia Chalkidikis and Koroneiki

in Greece. Other olive species such as O. ferruginea, O. verrucosa and O.
chrysophylla have been used in the past as rootstock. Furthermore, some other
species have been tested as rootstock, such as those of Fraxinus and Syringa
vulgaris. The stones for seed propagation derive from fruits harvested when
they are fully ripe, i.e. the period from October to January (northern
hemisphere) (Adakalic et al., 2004). Harvesting time affects the germination of
olive seeds (Rinaldi, 2000). Full ripening results in accumulation of storage
materials necessary for good germination. After fruit collection the separation
of esh proceeds, which can be achieved by two main methods: (i) hand
separation; and (ii) water soaking of olive fruits for 10 days in order to render
the fruit pulp soft, and afterwards esh removal by equipment that separates
the kernel from the esh.
Following de-stoning, the kernels are cleaned to remove the remaining
parts of the esh and dried in the air or in a forced-draught oven at low
temperature. Subsequently, the kernels are stored either under ventilation,
refrigeration at 10C or stratied with a sand:perlite (1:1) medium. Seeds
maintain their germinability for 1 year or, in some cases, for up to 3 years, but
with a small decrease in their germination percentage.
The seeds of olives require up to 4 years for complete removal of dormancy
(Lagarda and Martin, 1983; Voyatzis, 1995). However, during seed storage a
slow decrease in dormancy is observed. Ethylene in vitro promotes olive seed
germination (Lambardi et al., 1994).
The dormancy of olive kernels can be classied as below:
1. Mechanical or seed coat dormancy. Seed coats inhibit penetration of water
and air and do not permit embryo growth and expansion during germination
(Instanbouli and Neville, 1979).
2. Chemical dormancy (or embryo dormancy). This may be ascribed to
substances accumulating in the embryo (coverings, seed coats, endosperm)
which inhibit germination (Mitrakos and Diamantoglou, 1984). Removal of
the seed coat overcomes dormancy.
In order to remove the problem of dormancy the following techniques may
be used:
1. Mechanical scarication with a scarier reduces the thickness of the stone
and allows the entrance of water, and hence triggers seed germination.
2. Chemical treatment of kernels with concentrated sulphuric acid (H2SO4)
for 1 h, which decreases the tolerance of the endocarp and aids germination.
Kernels treated with H2SO4 germinate within 56 weeks. After this treatment,
a period of cold stratication is unnecessary for the germination of olive s
eeds. If kernels have been treated with sulphuric acid, the tip of the stone is
broken with forceps until the seed appears. Consequently, the kernels are
stratied in February and sowing follows at distances of 5060 cm between
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Chapter 14
rows and 5060 cm within rows. The seedlings are grafted into these sites
after 2 years. Also, for chemical treatment, 5% w/v of sodium hydroxide
(NaOH) could be used. Stones are immersed in 5% NaOH for 24 h, under
continuous stirring.
3. Water soaking of kernels for a period of 20 days. The water should be
replaced every 3 days.
4. Seed stratication in a sand:perlite (1:1) mixture in a cold room or outdoors
during winter.
Seed sowing and seedling transplanting

Seed sowing may be performed in several ways:
1. Use of cold frames or plastic containers.
2. Sowing outdoors in a well-drained soil with adequate moisture and aeration.
It is not necessary to provide darkness for good germination of olive kernels;
however, shading during summer is benecial. Seed sowing is conducted
in summer (kernels from the previous year) and germination begins after
2 months.
3. Use of seedbeds with a bottom-heat system, in order to increase the
germination percentage to 5070%.
4. Following seed coat removal the seeds are germinated in vitro in Petri dishes
or culture tubes. Seed disinfestation, with 2% sodium hypochlorite (NaOCl)
solution for 20 min and several washes with sterile distilled water, then follows.
5. Use of growth regulators. To overcome chemical dormancy seeds may be
treated with ethephon (200 mg/l), producing ethylene.
Whichever of the above methods is used, seedlings are transplanted to the
soil after a light trimming. Seedlings of uniform quality are selected for
transplanting in the nursery or in plastic bags. When the seedling base reaches
the thickness of a pencil it is ready for budding.
PROPAGATION BY CUTTINGS
When propagating by cuttings the formation of adventitious roots is very
important; these are roots arising from any plant part other than the root and
its branches. Adventitious roots are distinguished by two types: preformed roots
and wound roots. Preformed roots develop on a stem attached to the mother
plant, while wound roots appear after cuttings are prepared. The following
changes take place: (i) initially, the wound surface is covered with suberin,
which protects the wound from desiccation; (ii) subsequently, some living cells
divide and produce callus, while cells close to the vascular cambium and
phloem start to produce adventitious roots (Ozkaya et al., 1997). Callus is an
129
irregular mass of parenchymal cells, which are produced from young cells at
the base of the cutting. The rst roots frequently appear through the callus.
Callus formation is a prerequisite of adventitious root formation. However, in
many species callus formation is independent of rooting. In the case of the olive,
where layers of secondary xylem and phloem are present, adventitious roots
arise from the living parenchymal cells and also from other tissues such as
cambium, phloem and vascular rays. In some cultivars that are difcult to
root, the presence of a sclerenchymatous ring between the phloem and cortex,
outside the area of origin of adventitious roots, produces an anatomical barrier
to rooting.
In rooting of stem cuttings it is important to place the cuttings in the
rooting medium in the correct polarity. Stem cuttings form shoots at the distal
end (close to the shoot tip) and roots at the proximal end (close to the crown of
the plant). When we invert the position of cuttings (upside down) this does not
alter this tendency.
Factors affecting olive propagation by cuttings

Signicant differences exist between olive cultivars, in terms of propagation, as
listed below:
1. Selection of material for cuttings.
Physiological age (juvenility, maturation).

Time of year during which the cutting is prepared.
Type of wood.
Physiological condition of the mother plant.
2. Environmental conditions during the rooting period.
Water stress.
Light (intensity, quality and duration).
Rooting substrate.
Temperature.
3. Treatment of cuttings with various chemicals (growth regulators, fertilizers,

pesticides).
Juvenility
In the olive the use of juvenile leafy cuttings taken from the crown of the trunk
root much more readily than those taken from the top of the tree (Porlingis and
Therios, 1976). Experiments with leafy cuttings (cv. Chondrolia Chalkidikis)
indicate that the ability of cuttings to root decreases with increasing height above
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Chapter 14
the crown of the tree, i.e. by increasing the transformation from juvenile to
mature characteristics. Juvenile olive shoots are characterized by their short leaf
length and greater width, dark green leaves, change in phyllotaxy (two to three
leaves per node instead of two). Some procedures, such as severe pruning, provide
material with signicant rooting potential, since this prevents the transformation
from the juvenile to the adult form. The greater ability of juvenile olive cuttings to
root may be ascribed to increased phenol concentration in comparison with that
in mature plant material. Another possible reason is the greater concentration of
rooting inhibitors that accompanies ageing.
For better results it is necessary to maintain the mother plantings. These
plantings are maintained in the juvenile phase by spraying with growth
regulators such as GA3, cytokinin, TIBA or Alar. In olives, juvenile shoots are
obtained from sphaeroblasts, which are overgrowths on those stems with
meristematic tissues. Another method is to use mount-layering (stool beds) to
root those cultivars considered difcult to root.
Choice of plant material for cuttings

The physical condition and any previous treatment of the plant material may
exert a signicant effect on the rooting of cuttings. Such factors include
mineral nutrition, carbohydrate concentration and water stress. With regard
to mineral nutrition, an average concentration of N in the leaves allows
maximum rooting. High N results in excessive vigour and reduced rooting.
Such tissues are succulent and their carbohydrate accumulation is low. The
average N level determines the carbohydrate:nitrogen ratio; low N increases
this ratio and results in better rooting. The level of carbohydrates is important
for rooting. In order to obtain a high carbohydrate level the leaves of olive trees
should be healthy and well illuminated. Factors such as infection from
Cycloconium oleaginum resulting in leaf abscission, or very dense planting, lead
to shading and decrease the level of carbohydrates. However, adequate levels of
Zn increase the production of tryptophan necessary for IAA production and
therefore may improve rooting. On the contrary a high Mn level activates IAA
oxidase, leading to the destruction of IAA important for rooting and
decreases rooting.
Water content of the cutting material should be adequate. Water-stressed
cuttings encourage ABA production, leaf abscission and decreased rooting.
Regarding the type of cutting and its siting depends on the season: in early
summer basal (less succulent) cuttings are preferred while later (September),
those from the middle and upper parts of juvenile shoots are preferred. When
mature shoots are used for cutting production variations in rooting are
recorded, with the best rooting in cuttings taken from the basal part of the
shoot. When hardwood cuttings are used, cuttings with a small piece of older
wood at the base (heel) give better results.
131
The selection of season when preparing leafy cuttings is very important for
optimum rooting. Juvenile cuttings give optimum rooting irrespective of the time of
collection, while mature cuttings rooting extremely well under mist can be obtained
during late spring and summer. During winter, rooting is signicantly poorer.
Treatment of cuttings and stock plants

Adventitious rooting may be induced by the presence of various chemicals, such
as auxins (IAA, IBA, NAA, 2,4-D) and ethylene gas. The acid form of these
compounds is relatively insoluble in water and therefore they previously
should be dissolved in a few drops of ethyl alcohol before addition of water. The
salts of the above-mentioned auxins are preferable, due to their greater
solubility in water. For commercial-scale propagation it is more preferable to
use IBA or NAA. The auxin IAA is more sensitive to light and is destroyed
easily, while IBA and NAA are almost totally light stable. These latter compounds are also more tolerant to bacterial destruction and retain viability for a
long period of time. Use of high auxin concentrations in olive cuttings,
especially those from mature material, inhibits bud growth and no sprouting
occurs under mist propagation. This does not apply to juvenile material, where
the percentage of sprouting under mist is always very high.
Treatment of cuttings with fungicides results in better survival rates and
improved rooting. Suitable fungicides include Captan and certain systemic
fungicides such as Neotopsin, etc.
Wounding of the base of cuttings improves rooting, especially for those
derived from mature stock material. Wounding promotes cell division due to
auxin and carbohydrate accumulation in the wounded area, and exogenously
applied auxin is absorbed more readily than that applied endogenously.
Environmental conditions
High air temperatures during rooting increase water loss from the leaves. The
ideal air temperatures for rooting are 2527C during daytime and 15C at
night. Light exerts a strong inuence on rooting (duration, intensity, quality).
Many reports indicate that a relatively low light intensity given to both stock
plants and cuttings increases the percentage of rooting. This is in accordance
with the etiolation of stock plants or the base of shoots (Mencuccini, 2003),
which has a remarkable action in adventitious root formation, as occurs in the
method of propagation by mound layering. Furthermore, the photoperiod applied
to mother plants has a signicant inuence on rooting. A long photoperiod
increases carbohydrate accumulation and therefore rooting. Concerning light
quality, the light colour affects rooting. However, the active spectrum depends
on the species; in some it is orangered, in others blue.
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Chapter 14
The rooting substrate supports the cuttings and provides moisture and
oxygen at the base of the cuttings. The rooting substrate affects rooting
percentage and root characteristics due to its porosity, pH and the level of the
nutrients it contains. Rooting media with great porosity, such as sand or
perlite, give an unbranched root system, while very acid or very alkaline media
signicantly reduce rooting. Furthermore, the nutrients that the substrate
contains inuence rooting.
Types of cuttings
In olives propagation by cuttings is divided into the following three categories:
hardwood, semi-hardwood leafy and softwood (Khabou and Trigui, 1999). Of
these, the most important in olive propagation is the semi-hardwood leafy, and
less so hardwood.
Hardwood
These derive from mature hardwood, a method cheap and easy for
propagation. Today, in various parts of Greece these cuttings are also termed
grotharia. The necessary stock material is collected during pruning and derives
from healthy, vigourous and typical specimens of the cultivar trees. The
cuttings are prepared at the end of JanuaryFebruary from shoots 34 years
old and 2.55.0 cm in diameter. The shoots are divided into segments, each of
30 cm, and the leaves are removed. The cuttings are planted with their
physiological base downwards (true polarity). The base of the cutting is soaked
for 24 h in a solution containing 13 ppm IBA. Subsequently, the cuttings are
covered with moist material for 30 days at 1321C. During this period root
initials develop and, after 30 days, the cuttings are planted in the nursery, with
only 34 cm of their structure above the soil.
Leafy
Various factors affect rooting of leafy cuttings, such as: (i) the cultivar (Fouad et
al., 1990); (ii) the carbohydrate level (Rio et al., 1991; Ozkaya and Celik,
1999); (iii) hydrogen peroxide (H2O2) (Sebastiani et al., 2002a; Sebastiani and
Tognetti, 2004); (iv) endogenous auxins (Gaspar et al., 1994); (v) polyamines
(Rugini et al., 1992, 1997); (vi) free amino acids (Sarmiento et al., 1990); (vii)
light quality (Morini et al., 1990); (viii) hormones (Fernandes Serrano et al.,
2002); and (ix) other factors (Gaspar et al., 1997).
For propagation by leafy cuttings, two systems contribute signicantly, the
mist and the fog systems (Fouad et al., 1990). In the case of mist propagation,
cuttings are sprayed with water by an automatic system to maintain a lm of
water on the leaves, which reduces transpiration and lowers air and leaf
temperature. The resultant temperature decrease is by between 5.5 and 8.5C,
in comparison with leaves not sprayed under mist. With mist propagation,
133
transpiration is low, while light intensity is high. This increases the

photosynthetic rate and rooting. To avoid problems of high moisture, the mist
propagation system is operated only during the hours of daylight. The periods
of spraying are controlled by a timer or by an electronic leaf, hence spraying is
intermittent, water being applied at short and frequent intervals. The leafy
cuttings are usually planted during June, July, August and September and the
stock material is annual shoots, with a girth of 56 mm (see Table 14.2). The
apical softwood part of the shoot is removed. The length of leafy cuttings is
14 cm and their leaf number is four to six. The basal 1.5 cm of each cutting is
immersed in a 4000 mg/l solution of IBA for 5 s. Cuttings are planted in small
containers in a 1:1 mixture of perlite:peat, and roots are initiated under mist
propagation. Dipping of the cuttings into a fungicide helps to prevent disease
attacks. The time required for rooting and root hardening is 3 months.
Subsequently, rooted cuttings (see Figs 14.214.4) are planted into plastic
bags or small plastic containers and transferred to a shaded site (see Figs 14.5
and 14.6). Cuttings originate from annual shoots with either mature or
juvenile characteristics. In comparison with cuttings exhibiting mature
characteristics, juvenile cuttings initiate rooting faster and achieve a greater
rooting percentage, more roots per rooted cutting and greater fresh weight of
the root system per rooted cutting. The percentage of rooting follows a seasonal
variation, with a maximum in summer and minimum during autumn and
winter. The rooting percentage of juvenile cuttings is high and constant during
the whole year (Porlingis and Therios, 1976). The percentage rooting of
cuttings originated from the tree crown is greater in comparison with cuttings
from shoots close to the treetop. The rooting media used and their properties
are detailed below:
Fig. 14.2. Young adventitious roots a few days after emergence.
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Chapter 14
Fig. 14.3. Rooting of an olive micro-cutting under mist propagation.
1. Perlite is widely used as a rooting medium for leafy cuttings under mist, due
to its high porosity. It is used mostly in combination, at various proportions,
with peat moss.
2. Peat moss is combined with perlite in order to increase the water-holding
capacity of the mixture. The proportions of perlite:peat are either 1:1, 2:1 or
1:3.
3. Vermiculite is used as a mixture of equal parts of vermiculite and perlite.
This combination gives good results, achieving high porosity and adequate
moisture-holding capacity and cation exchange capacity.
The properties of perlite and vermiculite are given in Table 14.1.
The advantages of perlite include the following:
Improves aeration and drainage.

Is an inorganic material and does not disintegrate with time.
pH varies between 6.5 and 7.5.
Reduces the temperature variation of the soil.
Is free from diseases and weeds.
Is clean, light and easy to use.
Improves soil moisture and nutrient levels.
135
Fig. 14.4. Abundant root growth of leafy cuttings planted in plastic holder.
Table 14.1. The properties of perlite and vermiculite (from Therios, 2005a).
Characteristic
Perlite
Vermiculite
Inorganic material
pH
Sterilized material
6.57.5
When fresh it is sterilized
for re-use
Light; safe for staff
None
White
Excellent
Some samples contain

B and F
6.09.5
Sterilized when it is fresh
Purity, weight and safety

Scent
Colour
Aeration and drainage
Moisture and nutrient
elements
Easily available
nutrients
Light; safe for staff

None
Honey-coloured
Excellent when fresh;
disintegrates with time
Easily available
nutrients
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Chapter 14
Fig. 14.5. Olives planted in plastic pots and maintained outdoors.
Fig. 14.6. Olives planted in 2 l plastic pots.
137
Table 14.2. Rooting percentages of various cultivars under mist propagation (from
Therios, 2005b).
Variety
Greek varieties
Kalamon
mature
juvenile
Amphissis
Ladolia Patron
Kothreiki
Other varieties
Ascolana
Leccino
Coratina
Frantoio
Sevillana
Manzanillo
Pendolino
Mission
Rooting
success (%)
Date of
planting
IBA concentration
(ppm)
26 May
4000/5 s
60.6
95
68.1
68.1
93.7
26 May
26 May
26 May
26 May
26May
4000/5 s
4000/5 s
4000/5 s
4000/5 s
4000/5 s
99
93
93
96
85
80
67
66
15 July
26 July
26 July
26 July
15 May
15 July
10 February
15 July
4000/5 s
5000/5 s
2500/5 s
5000/5 s
4000/5 s
4000/5 s
100/24 h
4000/5 s
520
PROPAGATION BY SPHAEROBLASTS
Sphaeroblasts are ovular overgrowths of parenchymatic tissue located in the
tree crown or in old wood in the upper part of the tree. They contain latent buds
and are separated, cleaned from the wood and are divided into sections. They
are cut from the tree during the autumn and are subsequently stratied, in a
cool and dark room, into moist sand until March, and afterwards planted in the
nursery. The planting distances in the nursery are 3090 cm from each other
within rows. The sphaeroblasts are planted with their spherical part upwards
and are covered with soil. After 1.53 months the sphaeroblasts are sprouting
and remain in the nursery for 1 year.
PROPAGATION BY SUCKERS
These develop at the crown of the tree and their origin is from sphaeroblasts.
Suckers are taken from the tree when they have acquired an adequate root
system. In order to speed the rooting process the base of the tree is covered with
a thin layer of soil, encouraging root initiation. The rooted suckers are removed
in the spring from the mother plant, with some old wood, and planted in the
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Chapter 14
nursery. This method is slow and it cannot be used as a commercial method of

olive propagation. The use of suckers is a common means of propagation of
trees that have died after a severe frost. Furthermore, olive cultivars (e.g.
Kalamon) that are hard to root could be propagated by soft layer or mound
layer (Petridou and Voyiatzis, 1993, 2002).
PROPAGATION BY GRAFTING AND BUDDING

Grafting is the term used to describe the placing in contact of two portions or
organs from two different trees, in order for them to heal and grow as a
composite plant. The upper part is the scion and the lower one the rootstock.
Various types of grafting are used in olives and are known under various
names: chip and tongue grafting, side veneer grafting, saddle grafting, wedge
grafting, crown grafting and bark grafting. Also, in olives certain types of
budding are used, such as patch budding, ring budding and chip budding.
Many times we face the question of what is preferable a grafted plant or
a self-rooted olive plant? Substantial differences exist concerning the
morphology and development of the root system. Olive plants originated
from rooted cuttings develop a root system that is supercial but with a wide
radius. With regard to seedlings, these develop their roots from an axis of
20 cm, have a narrower angle and grow to greater length in the soil, which is
very important for sandy soils and dry areas. However, no signicant
differences were reported concerning productivity between grafted and selfrooted plants.
Grafting is recommended in the following cases:
1. For propagation of olive cultivars that cannot be propagated by cuttings.
One example is the Greek table olive Kalamon.
2. In using problematic soils (high calcium carbonate (CaCO3), high
moisture, salt stress and diseased) by using the appropriate rootstock.
3. To heal trunks damaged by rodents, diseases or agricultural machinery
(bridge grafting).
4. To change cultivar, especially in the case of a lack of pollinizers or when the
pollinizers are unsuitable.
5. To produce composite plants for plant physiology studies.
The graft union

Very few studies have been conducted concerning the anatomy of the graft
union of olives. When a scion is grafted to a rootstock the two parts should be
compatible. In fact, incompatibility is not common in O. europaea cultivars and
139
between olive cultivars and wild olive (O. sativa or oleaster). However,
incompatibility may occur when the olive is grafted on to other genera of
Oleaceae, such as Ligustrum, Forsythia, Syringa, etc., and plants will die after a
short period.
The graft union is completed by cells that develop after grafting. These cells
in contact differentiate into a vascular cambium producing xylem and phloem
elements and a complete vascular continuity by day 60.
A number of studies have been made on the healing of graft unions. The
usual sequence of events in the healing of a graft union is as follows:
1. Freshly cut scion tissue capable of meristematic activity is brought into
contact with cut stock in such a way that the cambial regions of both are in
close proximity. The temperature and relative humidity should be such as to
promote growth activity.
2. The outer layers of the cambial zone of both scion and rootstock produce
parenchymal cells called callus tissue.
3. Some cells of the callus differentiate into new cambium cells.
4. The new cambium cells produce xylem towards the inside and phloem
towards the outside, and establish a vascular connection between the scion
and stock, a prerequisite of efcient graft union. It should be stated that, in the
graft union, there is no intermingling of cell contents.
Temperature, moisture and other conditions during grafting

Temperature has a signicant inuence on callus tissue production. Outdoor
grafting operations performed late in the spring, when high temperatures may
occur, result in failure.
Moisture also has a pronounced inuence, since the callus tissue cells are
thin and not resistant to desiccation. This is common on healing olive grafts.
Air moisture levels below the saturation point inhibit callus formation.
Furthermore, oxygen is necessary at the graft union for the production of
callus tissue, since the rapid cell division is accompanied by high respiration
levels, requiring O2.
Growth activity of the scion and rootstock

Grafting depends upon the bark slipping. This means that the cambium cells
are actively dividing, producing thin-walled cells on each side of the cambium.
These newly formed cells separate easily, i.e. the bark slips. Initiation of
cambium activity in the spring results from the onset of bud activity after the
bud starts growing.
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Chapter 14
Polarity in grafting
It is essential that there is proper polarity in the graft union. In grafting, when
two stem pieces are put together the morphologically proximal end of the scion
should be inserted into the morphologically distal end of the rootstock.
However, in grafting a stem piece (scion) on a piece of root, as in the case of root
grafting, the proximal end of the scion should be inserted into the proximal end
of the root piece.
Selection of scion wood and grafting time

One-year-old vigourous shoots, cut from mature and healthy plants, are the
source of olive scion wood. The stock plants should be productive and free of
diseases and viruses. Furthermore, the stock plants are marked and scions are
cut from the same trees in a succession of years. These trees should be
controlled and maintained under good sanitary conditions. The scion wood
should be preserved for some days before it is used for scion preparations. The
storage of scion wood for long periods should be conducted at 34C in a
refrigerator. Preservation for short periods is achieved in a room with a lower
temperature than outdoors. In order to avoid dehydration, the scion stems are
placed with their bases in a bucket containing 25 cm water.
The best time for grafting depends on the cambial activity, i.e. easy
separation of the bark from the wood. This time coincides with the period
AprilMay. Grafting is unsuccessful in hot and dry weather. For a successful
grafting the following conditions are necessary:
1. Compatibility of rootstock and scion. This depends on the congeniality of
the two parts.
2. Intimate contact of the cambial regions of stock and scion. The two surfaces
should be held together tightly by wrapping. Rapid healing of the graft union is
necessary.
3. Grafting or budding should be performed when growth activity is observed
in both stock and scion.
4. Protection of the grafting area from drying out.
Methods of grafting
Cleft grafting
This method is adapted to topworking trees either in the trunk of a small tree or
in the scaffold branches of a larger tree (Troncoso et al., 1999). In topworking of
olive trees, this should be limited to branches about 2.510.0 cm in diameter.
Cleft grafting is successful if it is carried out in early spring. The scions are made
141
from 1-year-old wood, which should be used immediately or should be collected

in advance and maintained under refrigeration.
The cutting of the branch for grafting should be made at 90 to the central
axis. A large knife is used to make a vertical incision for a distance of 58 cm
down the centre of the branch to be grafted. The knife is inserted with the help
of a hammer. This incision should be in a tangential rather than in a radial
direction in relation to the centre of the tree. In each split two scions are
inserted, one on each side, in close contact with the cambium of the stock. The
length of each scion is 810 cm, with a diameter of 1012 mm and containing
two pairs of leaves. The basal part of each scion should be cut into a sloping
wedge, about 5 cm long. The scion is inserted into the split and, after removal
of the grafting tool, the pressure of the split stock holds the scion in position.
The bark of the stock is thicker than that of scion; therefore, the outer surface
of the scion is placed in an appropriate position to match the cambium layers of
the stock. Thorough waxing of the graft is necessary, and the top surface of the
stock should be entirely covered. The tops of scions should also be waxed.
Bark grafting
This grafting technique is very simple and rapid, and is performed on stock from
2.5 up to 30 cm or more in diameter. For olive trees, freshly cut scion wood can be
used, and several scions are inserted into each stub. In each scion a vertical
incision of 2.55.0 cm is made. The scions are inserted into the stub and are held
in place by wrapping string or adhesive tape. The wrapping material used after a
successful budding must be removed in order to avoid constriction of the grafted
area. All the cut surfaces and also the end of the scions should be waxed. A
team of three people is required, one preparing the plants, one for tying and one for
waxing. The output of such a team is in the order of 15002000 grafts per day.
With this method, bark grafting of wild olives is carried out in early spring
and the wild olives are converted to productive trees of selected cultivars. Wild
olives do not have a good taproot system and therefore do not possess tolerance
to water stress.
Whip grafting
This method is extensively used to graft small seedlings of O. sativa and is also
used in making root grafts. With this method, cambial activity is very efcient
and the union is very strong. It is recommended that both scion and stock are
equal in diameter. A long, sloping incision (56 cm long) is made at the top of
the rootstock. An identical cut is made at the base of the scion. Another cut is
made one-third of the length from the top to the base of the rst cut in the
rootstock and scion. The two parts (scion and rootstock) are inserted together
in such a way that the cuts (tongues) interlock. Afterwards, the graft union is
tied and covered with wax.
Other methods of grafting in olives, but of less signicance, include the
following:
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Chapter 14
Splice grafting
This is the same as whip grafting, with the exception that the cut is not made in
the stock and scion.
Wedge grafting
This can be performed in late winter or early spring. With a sharp knife a Vwedge is made in the stock, 5 cm long. With a screwdriver the V-shaped chip is
removed and in the space created the scion is inserted. The scion is 1013 cm
long and 1012 mm thick, with two pairs of buds and four leaves. After the
scions are put in place in such a way that the cambiums are in contact, the cut
surfaces are waxed.
Bridge grafting
This is used when the trunk has been injured by agricultural machinery,
winter damage or disease; in the case of bark damage, if the damage is extensive
the tree will die. This type of grafting is conducted early in spring, i.e. the period
when the bark is slipping. The damaged bark is removed down to the level of
healthy tissue. Cuts are made in the bark at both top and bottom of the wound
and scions are inserted into each slot. The scions are xed in place by nails, and
the unions at top and bottom are covered with grafting wax.
T-budding (shield budding)
T-budding is the most common method of budding in nurseries. The rootstock
has a diameter of 625 mm and should be actively growing in order for the
bark to separate easily from the wood. In the rootstock, horizontal and vertical
cuts of 2.53.0 cm are made. The scion consists of a shield of bark containing
one bud. The shield of bark is inserted by pushing it downward under the two
aps of bark, and the bud union is tightly tied with wrapping material or plastic
tape. Two methods of preparing the shield are used; the rst one contains wood
and the second one does not.
Patch budding
In this method, a rectangular patch of bark is removed from the rootstock and
in its place is placed a patch of the scion bark of the same dimensions (Krueger
et al., 2004). This method is technically more difcult than T-budding and is
used in trees with thick bark, like olives. Patch budding requires less scion
wood, it is fast and can be conducted over a longer period. Patch budding
requires that both scion and rootstock have good cambial activity, and
therefore bark that slips. The time for patch budding extends from April to
September, and this period is longer for wood of smaller diameter. The scion
wood consists of vigourous 12-year-old shoots with the diameter of a pencil or
greater. The leaves from the scion are removed and the scions are used
143
immediately after collection. In the rootstock, a strip of bark to prevent dying of

the rootstock is maintained. For the cutting of patches special knives, doublebladed, have been devised to remove the bark segment from the stock and the
bud stick. Using the double-bladed knife, one makes two horizontal cuts in the
rootstock, then a vertical one at one end of the horizontal cuts and the bud is
slipped into place. Afterwards, the inserted bud should be wrapped. In the case
of patch budding of thick scaffold branches, the stock bark will be thicker than
the bark of the inserted bud patch. This makes wrapping difcult when aiming
to hold the bud tightly. Under such circumstances, it is necessary that the bark
of the stock be trimmed around the bud patch in order that both components
be the same thickness; thereby, the wrapping material will hold the bud patch
tightly. For wrapping, adhesive tape may be used to prevent the entrance of air
under the patch, which could lead to the death of the patch. The bud, however,
is not covered during wrapping.
Another method is to use bark from the rootstock to hold the bud in place.
The required time for healing is 1 month at a minimum, and it is often best to
wait longer before forcing the buds. If budding is successful, the rootstock limb
is cut above the inserted buds in order to force the buds to grow. Yet another
way is to girdle the trunk above the inserted bud. The buds may remain
dormant during the year of budding and are forced the following spring. On the
same tree the scaffold branches are not all cut at the same time, but one to two
scaffold branches are left on the southerly side of the tree as nurse shoots.
These nurse scaffolds are removed after 2 years, when the buds provide shoots
of adequate length.
IN VITRO CULTURE OF OLIVE TREES

This technique involves propagation under controlled conditions (temperature,
light quality, intensity and photoperiod) (Amiri, 2004) of olive cultivars, to
produce genetically homogenous, pathogen-free plants (Rugini, 1984; Caas
and Benbadis, 1988; Leifert et al., 1992; Mitrakos et al., 1992; Mencuccini and
Rugini, 1993; Leva et al., 1994, 2002; Ozkaya et al., 1997; Chaari-Rkhis et al.,
1999; Briccoli Bati et al., 2000; Garcia-Frriz et al., 2000; Pritsa and Voyiatzis,
2002; Zuccherelli and Zuccherelli, 2002; Lambardi and Rugini, 2003; Giorgio
et al., 2006).
The main aims of micropropagation are the following:
Rapid propagation of cultivars difcult to propagate by cuttings.

Production of disease-free plants.
Genetic improvement.
Cryopreservation of variable germplasm (Martinez et al., 1999; Lambardi
et al., 2002).
Organogenesis.
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Chapter 14
Somatic embryogenesis (Rugini and Tarini, 1986; Orinos and Mitrakos,

1991; Leva et al., 1995, 2006; Rugini, 1995; Lambardi et al., 1999).
Micro-grafting.
Synthetic seed production.
Organogenesis is the process by which undifferentiated cells differentiate

and produce various organs such as roots, buds, owers, etc. Differentiation
originates from the cells of a tissue or from the cells of a callus that was formed
(indirect organogenesis). Factors affecting regeneration include the cultivar, the
substrate used and leaf position on the shoot.
Stages of micropropagation
The micropropagation process includes the following stages:
1.
2.
3.
4.
5.
Excision and collection of explants from donor plants.

Disinfection of explants and culture initiation.
Shoot proliferation.
Shoot elongation and rooting of explants (Antonopoulou et al., 2006).
Acclimatization of rooted explants and transfer to the eld.
Disinfection of explants and culture initiation

The explants originate from stock plants of good growth and sanitary
condition and with characteristics representing a certain cultivar and having
genetic stability. The mother plants are maintained in the orchard or in a
greenhouse. It is not suggested that plant material is used directly from olive
groves, since this material is highly contaminated and contains a lot of tannins,
leading to tissue oxidation when this material is cultivated in vitro. To reduce
these problems, the mother plants are planted in containers and are maintained in a greenhouse. The stock plants are pruned to achieve rejuvenation,
with explants characterized by juvenile characteristics and minimal oxidation.
The stock plants are sprayed with systemic fungicides, insecticides and
antibiotics (streptomycin sulphate) every week to control their diseases. For
better results avoid moistening the leaves by overhead sprinklers. From the
treated stock plants are cut vigourous, tender, apical twigs, which are divided
into uninodal segments (12 cm, or longer, with leaves).
The disinfestation of the olive plant material is achieved by treating with
sodium hypochlorite (NaOCl), at various concentrations and exposure times in
order to select the most effective combination. Previously, the stock material
was rinsed in running tap water, before sterilization. To avoid tissue browning
due to phenolic oxidation, the culture medium is supplemented with anti-
145
oxidants such as ascorbic acid (1020 mg/l), or the tissue is pretreated in

sterile water containing antioxidants, for 30 min. Another technique to avoid
oxidation is frequent subculturing of explants very often until the substrate
does not darken. After sterilization, the explants are transferred aseptically to
test tubes, one per tube.
Shoot proliferation
The new shoots (proliferation) arise from axillary and adventitious buds due to
exogenous application of cytokinin-like substances (Mencuccini and Rugini,
1993). Various substrates for proliferation may be used, such as MS (Murashige
and Skoog medium), WPM (Woody Plant Medium) or OM (Olive Medium) (see
Table 14.3).
Table 14.3. Composition of Olive Medium (OM).

Compound
Macronutrients
KNO3
NH4NO3
Ca(NO3)24H2O
CaCl22H2O
KCl
MgSO47H2O
KH2PO4
Micronutrients
FeSO47H2O
Na2EDTA
MnSO44H2O
H3BO3
ZnSO47H2O
Na2MoO42H2O
CuSO45H2O
CoCl26H2O
KI
Vitamins
Myo-inositol
Thiamine-HCl
Pyridoxine-HCl
Nicotinic acid
Biotin
Folic acid
Amino acids
Glycine
Glutamine
Concentration (mg/l)
1100
412
600
440
500
1500
340
27.8
37.5
22.3
12.4
14.3
0.25
0.25
0.025
0.83
100
0.5
0.5
5.0
0.05
0.5
2
2194
146
Chapter 14
For satisfactory growth, olives require both NO3 and NH4+, inorganic
nitrogen sources and glutamine. The Ca:N ratio is very important; a ratio of
about 1:11 is used in the OM. The OM contains macronutrients, micronutrients, vitamins and amino acids. As a carbon source, sucrose or mannitol
is used. Mannitol has been proved more effective for olive shoot proliferation
at a concentration of 30 g/l; agar concentration is 0.50.8%. The pH of
the medium is adjusted to 5.75.8 before autoclaving at 121C for 20 min.
The OM also contains growth regulators. The best cytokinin for olive shoot
proliferation is zeatin, at concentrations from 0.5 to 4.0 mg/l, depending on
the cultivar. Other cytokinins such as 6-(,-dimethylallylamino)-purine (2iP)
or BA (6-benzyladenine) are not very effective for olive shoot proliferation,
since very short and thin shoots tend to be produced. Other cytokinin-like
substances such as thidiazuron (TDZ) may be included in the substrate. For
olive shoot proliferation glass jars of 500 cm3 are used. These jars are closed
with glass lids and wrapped in plastic membrane. In each jar, 100 ml of agar
medium is added and ten to 20 explants. The jars are maintained in the growth
room at 2325C, 16 h of photoperiod and 50 mol/m2/s of PAR supplied by
cool white or day light uorescent lamps. Every 34 weeks the explants are
transferred to a fresh substrate.
Shoot elongation
The shoots produced by proliferation are very short and not yet ready for the
rooting stage. Elongation of short shoots is promoted by transferring the
explants to another medium, similar to the previous but containing GA3
(2040 mg/l). When the plantlets obtain an appropriate length (four to ve
nodes) they are ready for the rooting stage. The media used for proliferation
and rooting depend on the cultivar, and Table 14.4 gives the appropriate media
for each cultivar as reported in the literature.
Rooting of explants
Rooting is achieved on the same shoot proliferation medium by decreasing the
macro-element concentration to one-third or one-quarter of the initial.
Sucrose was added at 2% concentration, plus 1 mg/l IBA or NAA. Rooting can
be improved by maintaining the base of the shoots in darkness or by adding
putrescine (1 mM) plus auxin to the rooting substrate. Auxin could also be
applied by dipping the basal part of micro-cuttings in an IBA solution
(100200 ppm, for 10 s) and afterwards planting in auxin-free media. Rooting
could also be achieved with the Agrobacterium rhizogenes strain NCP BB 1855
by wounding the shoot base with a blade contaminated by the bacteria.
Acclimatization of plantlets
Plantlets are removed from the asks under the laminar air ow and their root
system is washed to remove the gel, then dipped in a fungicide solution.
147
Table 14.4. Micropropagation of olive cultivars.

Cultivar
Arbequina
Dolce Agogia
Frantoio
Carolea
FS-17
Kalamon
Moraiolo
Maurino
Nocellara Etnea
Proliferation medium
Rooting medium
OM (zeatin)
OM (IBA or NAA)
50% MS (zeatin + IBA + GA3)
50% Knop/Heller (NAA)
OM (zeatin + 2iP)
BN (NAA or IBA)
50% OM (zeatin)
50% OM (NAA/IBA + Putr)
mOM (zeatin + 2iP)
50% MS (NAA)
WPM (BAP + IBA + GA3)
WPM (IBA)
OM (zeatin or 2iP)
(NAA or IBA)
mMS(zeatin or TIBA)
mMS (IBA-dip method)
mOM (zeatin)
mOM (NAA)
Stage
Rooting
Rooting
Rooting
Rooting
Rooting
Rooting
Rooting
Rooting
Rooting
Putr, the polyamine putrescine; mOM, modied Olive Medium; OM, Olive Medium
formulation; WPM, Woody Plant Medium
Afterwards, the plantlets are planted in pots containing an easily draining

substrate, consisting of perlite, vermiculite or peat, ideally 1:1:1. To avoid
contamination the plantlets, plus the substrate, should be sprayed with the
fungicide every week. Another method is to use inorganic substrate alone
such as perlite or vermiculite and to moisten it with water containing a very
low concentration of X-L-60 fertilizer. The pots with the plantlets are
transferred to a bench and covered with transparent plastic lm for
acclimatization. To avoid overheating of this space the temperature should be
controlled or this space should be shaded. Gradually, the relative humidity of
this space is reduced by opening the plastic for longer periods. After
acclimatization, the plantlets are grown for 1 year in the nursery to attain an
adequate size for planting in the olive grove.
Somatic embryogenesis
Plant regeneration via organogenesis and somatic embryogenesis in the olive is
a useful tool for breeding and somaclonal selection (Rugini and Tarini, 1986;
Orinos and Mitrakos, 1991; Leva et al., 1995, 2006; Rugini, 1995; Lambardi
et al., 1999). Regeneration via somatic embryogenesis has been reported using
cotyledonary tissues (Orinos and Mitrakos, 1991; Leva et al., 1995), immature
zygotic embryos (Leva et al., 1995; Garcia et al., 2002) and immature embryo
callus (Orinos and Mitrakos, 1991) in different olive cultivars.
Of the various explant sources (leaf blades, leaf petioles, hypocotyls of
germinated seeds and roots of germinated seeds) roots gave the highest callus
induction. Somatic embryogenesis was induced from root callus on
embryogenesis induction medium containing 5.0 M 2,4-D, 0.5 M kinetin
and 5.0 M NAA, in darkness. Embryo regeneration was achieved by
transferring the callus to a medium containing varying concentrations (0, 5.0,
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Chapter 14
10.0 and 15.0 M) of 2iP, BA, thidiazuron (TDZ) and kinetin. Sucrose evoked
higher embryogenesis than either fructose or glucose. Sorbitol and mannitol
reduced embryogenesis. Somatic embryos were rooted by transferring them to
a hormone-free medium.
Immature embryos possess a high embryogenetic capability, as shown in
Frantoio and other cultivars (Rugini, 1988). Furthermore, 75-day-old
embryos were able to exhibit embryogenesis.
The study of the origin of somatic embryos (SEs) is of great importance
both for the possible appearance of somaclonal variations and for their
utilization in genetic engineering techniques.
Somatic embryogenesis occurs either indirectly from callus or directly from
cells of organized tissues. Morphogenetic masses develop from both mature
tissues (petioles) and originated primary embryos. By subculturing primary
embryos, highly efcient continuous cycles of direct secondary embryogenesis
were obtained. Histological observations showed early evidence of embryonic
primordia as densely stained groups of ve or more meristematic cells, located
mainly in the epidermal layer of the primary embryos. These primordia evolved
into globular embryos that grew to the cotyledonary stage, through the heartshaped and the torpedo-shaped stages.
Other micropropagation techniques for the olive are described below.
Synthetic seed production

Somatic embryos or apical buds can be encapsulated in a substrate (sodium
alginate) for protection and survival. These seeds can be used for
micropropagation or for preservation of the genotype. Lambardi and Rugini
(2003) reported a protocol for encapsulating SEs of the cv. Canino. At least
49% of synthetic seeds remained viable after storage at 4C. Synthetic seeds
can be handled, stored or germinated in a way similar to that for a normal seedderived seedling.
Germination of synthetic seeds
Unlike a zygotic embryo inside a true seed, the somatic embryo is heterophylic
and may lack nutritional reserves. Therefore, the embryo culture medium has
to contain carbohydrates which, under non-sterile conditions, will favour
development of microorganisms. In order to monitor the conversion of SEs to
plants we have to follow two steps: (i) coating of the SE, which represents the
rst substrate for the culture of the young plantlet; and (ii) sanitary protection
of both the embryo and the developing plantlet. These may be achieved by
several means:
1. The use of micro-capsules that progressively release sucrose into the alginate
beds.
149
2. The use of self-breaking beds. Alginate beds obtained after hardening are
rinsed thoroughly with tap water and then immersed in a monovalent cation
solution followed by another rinsing with running tap water. After sowing in
moist condition, such gel beds gradually swell, become brittle and nally split
spontaneously, thus helping avoid oxygen deciency.
3. Pharmaceutical-type capsules appear to be an efcient coating system for
the production of synthetic seeds, allowing germination and subsequent
development of plantlets. The capsule is lled with 500 l of germination
medium containing gelrite, cotton or vermiculite. Finally, a torpedo-shaped SE
is placed on the internal medium without special sterilization. The capsule is
closed with a cap. Pharmaceutical-type capsules provide the nutritional
requirements of germinating somatic embryos by limiting sugar loss during
planting and germination.
Protoplast culture
The protoplasts isolated from olive organs or tissues can regenerate cell walls and
divide and form colonies and cells, followed by plant regeneration under proper
culture conditions (Adiri, 1975; Mencuccini, 1991; Perri et al., 1994a,b).
The regeneration process from protoplast to plant can be divided into
several phases: (i) cell wall regeneration; (ii) initiation of sustained divisions;
(iii) cell colony and callus formation; and (iv) plant regeneration from these
tissues. Viable protoplasts from seedlings (hypocotyls, cotyledons or cultivarmicropropagated leaves) were obtained by 1.5% Driselase after incubation
with 0.6 mM mannitol and 500 mg/l each of calcium chloride (CaCl2)2H2O
and potassium chloride (KCl). Some divisions were recorded in a BN (a nutrient
medium), which was supplemented with NAA, 2,4-D and zeatin riboside
(Rugini, 1986). Furthermore, Caas et al. (1987) separated protoplasts from
cotyledons by addition of 0.03 mM ornithine.
Growth regulator is the most critical medium component for protoplast
culture. In order to induce sustained divisions, an auxin-type growth regulator
and a cytokinin are generally required. The type and concentration of the
growth regulators vary with variety. In addition, plant hormones have some
effect on protoplast culture. In many cases glucose seems to be a better carbon
source and osmoticum (a controller of water movement, or osmosis) than
other sugars. The most frequently used techniques are: liquid culture, liquid
over agar (or agarose) culture and protoplast-embedded method.
Cryopreservation
Cryogenic methods have been applied to olives for long-term conservation of
germplasm (Lambardi et al., 2002). A 30% survival (Martinez et al., 1999) was
150
Chapter 14
observed for Arbequina shoot tips after their desiccation to 30% moisture
content and direct immersion in liquid nitrogen (196C), followed by a period
at room temperature. The shoot tips that survived remained green and started
to grow after 4 weeks. Furthermore, Canino cultures, with SEs at various
stages of development, can be cryopreserved by vitrication (Lambardi et al.,
2002), with a high percentage of the cryopreserved cultures surviving. These
cultures had a good proliferation rate and morphogenetic potential.
15
IRRIGATION OF THE OLIVE
INTRODUCTION
Water is fundamental for the physiological functions of plant cells and organs,
and it also represents a signicant proportion of the protoplast. Water
represents 8590% of the weight of all living tissues. When the water content
of cells is reduced, physiological activity is reduced and plants begin to wither.
Subsequently, and following resumption of water supplies, the plant returns to
its initial physiological condition. The water content of the various parts of
olive trees is given in Table 15.1.
The water content of olive plants varies, due either to water absorption via
the roots or from water loss by transpiration. The water required for the
physiological functioning of plants is less than 5% of the total quantity of water
absorbed: most of the absorbed water is lost in the form of transpiration. The role
of transpiration is in maintaining the thermal balance of plants, since
transpiration absorbs heat and cools the plant. The transpiration quotient is the
amount of absorbed water (l) to produce 1 kg of dry matter. Factors affecting
water consumption are the cultivar, the relative humidity, wind, temperature
and sunshine. Soil factors, such as fertilization, affect the transpiration rate.
Trees grown in fertile soils require less water. The water needs of olive trees in
areas with a precipitation of 450650 mm/year are completely fullled. When
precipitation is > 650 mm/year, olives can be replaced by other, more efcient,
crops. In contrast, when precipitation is < 450 mm/year, irrigation is necessary.
Table 15.1. The percentages of water, organic matter and ash of olive wood, leaf
and fruit.
Component
Water
Organic matter
Ash
Wood
Leaf
Fruit
32
66
2
54
43
3
53
45
2
151
152
Chapter 15
In solutions with a high concentration of nutrient elements, the needs of

olive plants are covered by the absorption of a smaller quantity of H2O.
Ignoring transpiration, to calculate the water requirements of olive trees, water
loss from the soil surface is included. The sum of transpiration plus water loss
from the soil surface by evaporation is termed evapotranspiration.
Water availability is fundamental for starch hydrolysis and contributes to
cell turgor, which controls the stomatal mechanism. If the leaf is not turgid
then the stomatal apertures are closed, and this reduces both CO2 supply and
the rate of photosynthesis. Therefore, water availability, stomatal opening, Pn
and nal yield are closely linked. Furthermore, water is a signicant solvent for
photosynthesis, and in combination with CO2 produces carbohydrates
according to the equation:
nCO2 + nH2O nO2 + Cn(H2O)n
Another role of water is as a solvent of nutrient salts and affecting their
transport to cells and tissues. The soil solution and nutrient elements are easily
absorbed from the plant providing that the osmotic pressure of the solution
within the plant counteracts the force of soil nutrients. When soil water is
retained by the soil with a force greater than the force with which it is
absorbed, the plant withers through lack of water.
WATER QUALITY
Rainfall is traditionally the main water supply in many areas of the world in arid
and semi-arid regions. In these areas, irrigation from underground sources
contains excess salts, affecting plant growth (Klein et al., 1994). Furthermore,
the quality of surface water is sometimes compromised by its mixing with poorquality drainage water. A serious problem nowadays is the over-pumping of
groundwater, which lowers the water table and leads to the entrance into
aquifers of sea water. The salt content of irrigation water is usually expressed in
terms of conductivity (Ecw); the units are dS/m at 25C and measurements are
conducted by using conductivity meters. Furthermore, the water quality for
irrigation and the evaporation from the soil surface lead to salt accumulation in
the root zone. The consequence of this is that the Ec of the soil solution is
signicantly higher than that of the irrigation water. The reduced yields in olives
due to salinity can be predicted when the Ec of a soil solution reaches
approximately 36 dS/m. The suitability of irrigation water depends on the total
amount and the type of salts present. Sodium, chloride and boron ions at high
concentrations are toxic. The suitability of irrigation water also depends on the
concentrations of Na, Ca and Mg (me/l), which are used to determine the specic
absorption rate with sodium adsorption ratio (SAR), according to the equation:
SAR = (Na)/(Ca + Mg)/2
Irrigation of the Olive
153
SAR values greater than 3 lead to breakdown of soil aggregates and

dispersion of clay minerals; such soils are known as sodic soils. The guidelines
for determining irrigation water quality are given in Table 15.2.
WATER REQUIREMENTS
Most of the global olive grove hectareage is not irrigated. Furthermore, in areas
where oil or table olive cultivars are under irrigation the water supply is scarce.
However, the new super-high-density plantings require irrigation, and efcient
water use is very important. The water needs of olives are a function of
Table 15.2. Characteristics of water quality for irrigation (from Ayers and Westcot,
1985).
Degree of restriction on use
Irrigation problem
Measurement
None
Slight to
moderate
Severe
Salinity
Ecw (dS/m)
TDs (mg/l)
Ecw (dS/m)
SAR (03)
SAR (36)
SAR (612)
SAR (1220)
SAR (2040)
< 0.7
< 450
< 0.5
> 0.7
> 1.2
> 1.9
> 2.9
> 5.0
0.73.0
4502000
< 0.5
0.70.2
1.20.3
1.90.5
2.91.3
5.02.9
> 3.0
> 2000
< 0.2
< 0.2
< 0.3
< 0.5
< 1.3
< 2.9
SAR
<3
39
>9
meq/l
<3
>3
meq/l
<4
410
> 10
meq/l
mg/l
<3
< 0.7
>3
0.73.0
> 3.0
mg/l
mg/l
Normal range
6.58.4
<5
< 1.5
530
1.58.5
> 30
> 8.5
Inltration
Specic ion toxicity

Sodium (Na)
Surface
irrigation
Sprinkler
irrigation
Chloride (Cl)
Surface
irrigation
Sprinkler
irrigation
Boron (B)
Miscellaneous
ions
Nitrate (NO3N)
Bicarbonate (HCO3)
pH
154
Chapter 15
evaporation (E) of water from the orchard soil and the canopy transpiration
(T). Hence, the required water is the sum of these two and is named
evapotranspiration (ET) (Testi et al., 2006). Factors affecting water use are:
solar radiation, temperature, humidity and wind speed. From the total
precipitation only 5060% is stored in the soil, while the rest is subject to
evaporation. The intensity and duration of precipitation determines the
percentage of water stored in the soil, and the relationship between them is
negative.
The rate of water evaporation from the soil surface is high when the soil is
irrigated and decreases as the soil dries out. Therefore, the greater the orchard
area that is wetted the greater the ET is. The modern trend in irrigation is
localized, such as drip irrigation. Planting cover crops increases ET, and their
use is avoided in areas with scarce irrigation water due to the high cost.
Transpiration is affected by various factors, such as total leaf area, solar
radiation intensity and duration, wind speed, etc. The total leaf area is affected
by the size of the tree canopy, the density of planting and the size and health of
leaves. The percentage of the orchard soil shaded by the olive trees correlates
well with the ET.
For ET estimation two methods are used (Testi et al., 2006). The rst of
these measures water loss from a round metal pan 1.2 m in diameter and
25 cm in depth. The pan is placed in an irrigated area of cut grass. Every day
the water loss from the pan is measured, the values being known as Epan.
These values are measured at meteorological stations. In the second method,
the modied Penman equation is used, which is based on measurements of solar
radiation, temperature, relative humidity, wind speed and direction. The values
calculated give an estimation of water use for a close-cut grass referred as ETo.
This method gives a better estimation of water use than Epan. However, these
values are only an estimation, and research is necessary in order to determine
the actual water requirements of various crops (ETc). The relationship between
ETo and ETc is constant, and the ratio ETc:ETo is used to develop crop
coefcients (Kc) (Michelakis et al., 1994; Orgaz et al., 2006). For olives in
California, USA, a Kc of < 0.65 leads to water stress of mature olive trees; a
value of 0.75 produces the best results and veries efcient water use (see
Table 15.3).
A new crop coefcient, Kc = Etc/ETo, can be calculated as the sum of three
components: tree transpiration (Kp), evaporation from the soil (Ksl) and
evaporation from the areas wetted by the emitters (Ks2) (Orgaz et al., 2006;
Testi et al., 2006). With the method of Orgaz et al. (2006) the calculation of the
needed irrigation amount (IA) is improved since various factors, such as soil,
plant and weather, are taken into account (Fernndez, 2006). The K
coefcients, as calculated by Orgaz et al. (2006), are presented in Table 15.4.
The ETo ranges between 1000 and 1400 mm/year for an average rainfall
in the Mediterranean region, equal to 500 mm/year, and for mature olive trees
under localized irrigation the ETc is 600700 m3/1000 m2, so 300400
155
Table 15.3. Average water requirements for clean-cultivated olive orchards in the
Sacramento area, California (from Beede and Goldhamer, 1994).
Month
January
February
March
April
May
June
July
August
September
October
November
December
Total
ETo (mm/month)
Kc
30.4
40.6
73.7
116.8
154.9
185.4
216.0
185.4
137.1
91.4
40.6
25.4
0.75
0.75
0.75
0.75
0.75
0.75
0.75
0.75
0.75
0.75
0.75
0.75
ETc (mm/month)
22.86
30.48
53.34
86.36
116.84
139.70
162.56
139.70
121.14
68.58
25.40
29.32
1297.1
Table 15.4. Water requirements of an olive orchard with mature Picual trees
planted at 10 m 10 m in southern Spain (from Orgaz and Pastor, 2005).
Month
January
February
March
April
May
June
July
August
September
October
November
December
Kp
KS1
KS2
Kc
ETo
(mm/month)
ETc
(mm/month)
0.18
0.19
0.20
0.23
0.27
0.32
0.32
0.31
0.28
0.31
0.28
0.18
0.67
0.65
0.40
0.25
0.13
0.05
0.04
0.05
0.18
0.38
0.68
0.72
0.00
0.00
0.00
0.04
0.03
0.03
0.03
0.03
0.03
0.04
0.00
0.00
0.85
0.84
0.60
0.51
0.43
0.40
0.39
0.38
0.49
0.73
0.96
0.90
33.2
45.9
87.1
110.4
154.1
169.5
210.8
182.3
122.1
80.6
42.6
29.6
28.2
38.6
52.4
56.8
66.8
67.3
81.3
70.1
59.9
58.8
40.9
26.9
Kp, coefcient related to tree transpiration; KS1, coefcient related to soil evaporation;
KS2, coefcient related to evaporation from the soil surface wetted by the emitters;
Kc, crop coefcient; ETo, potential evapotranspiration; ETc, actual water requirement.
m3/1000 m2 must be applied by irrigation. These values are not applied for
super-high-density (SHD) plantings or for young olive trees. According to
Grattan et al. (2006), with regard to SHD plantings of Arbequina I-18 (1.5 m
3.9 m), the ETc was 600 m3/1000 m2, while the required irrigation was 75%
of ETc, i.e. 450 m3/1000 m2.
156
Chapter 15
THE NEED FOR IRRIGATION

Olive leaves are covered on the lower surface with peltate trichomes which
protect them and reduce transpiration. This permits olive cultivation in very
dry areas. However, for good productivity the application of water is necessary.
Irrigation is essential in the following circumstances:
The rainfall is inadequate.

Precipitation distribution is not appropriate. Therefore, water shortage is
recorded in the critical periods during spring and summer.
In light-textured soils, with a low water-retaining capacity.
Irrigation is necessary in both table and olive oil varieties. However,

irrigation is more important in table olives to achieve large fruits in dense and
high-density plantings. Table olives should be irrigated during the third stage
(cell expansion) of fruit growth to increase their size. In contrast, overirrigation increases shoot growth, produces waterspouts and increases the
sensitivity of vegetative growth to winter frosts.
The methods applied for olive orchard irrigation include ood, furrow,
sprinkler, surface, sub-surface and drip irrigation (Fereres and Castel, 1981;
Bonachela et al., 2001). For drip irrigation one or two driplines are used per row.
THE ROOT SYSTEM OF AN OLIVE TREE

Olive trees do not have a dominant taproot system, and they develop many
lateral roots. The root system is shallow and is conned to the top 0.71.0 m of
soil; the lateral root system exceeds the projection area of the canopy. The root
pattern of the olive indicates that this system is not efcient in an avoidance
response to drought, since it is shallow and cannot reach water from greater
depths during dry periods. Root length increases under water stress and is 2.5
times longer than on irrigated trees. When olive trees are water-stressed for a
long period it is important to supply water to the entire root system (Bongi and
Palliotti, 1995). Experiments with split root systems and irrigation at two
different values of soil water potential indicated that olive plants grew less even
when a small portion of the root system was exposed to low , in comparison
with plants where both portions were irrigated with a high soil water potential.
This can be ascribed to the production of ABA by the stressed root system and
its transport to leaves (Zhang and Davies, 1989).
IRRIGATION AND DAILY VARIATION IN TRUNK GIRTH

During periods of water stress, olive trees experience a reduction in
transpiration, stomatal conductance and net photosynthesis (Angelopoulos et
157
al., 1996). However, environmental factors affect H2O and CO2 exchange to
varying degrees, causing variations in water use efciency. Water stress limits
stomatal conductance, photosynthesis, non-stomatal factors and assimilation
(see Figs 15.115.4). Sap ow patterns showed a steep diurnal morning
increase and a pronounced seasonal reduction throughout summer. The daily
alterations in stomatal conductance in olive leaves (cm/s) are presented in
Fig. 15.1. Reduction in soil water from 100% of evapotranspiration to 66%,
and afterwards to 33%, signicantly reduced stomatal conductance. The
maximum stomatal opening was recorded at 09.00 h and the minimum at
15.00. With regard to water potential, this was reduced (more negative values)
at 12.00 h, but later was increased due to stomatal closing (see Fig. 15.2).
Measurement of Pn in the cultivars Koroneiki and Mastoides indicated
that the maximum photosynthetic rate (Pn) was 1214 mol/m2/s (see Fig.
15.3). Furthermore, when a decrease in water potential value () was
observed, the pressure potential was reduced (see Fig. 15.4) in those cultivars.
The changes in values in eight olive cultivars indicate varying
osmoregulatory capacity. Cultivars whose value decreases rapidly under salt
stress conditions such as Chondrolia Chalkidikis, Manzanillo and
Amygdalolia are able to absorb water under adverse environmental
conditions and to adjust osmotically fairly rapidly; therefore, these cultivars
suffer less. In contrast, cultivars such as Megaritiki and Amphissis, with a
constant leaf value as the of the soil solution is reduced, suffer more under
conditions of increased salinity (see Table 15.5). The olive tree is characterized
by its ability to adapt to water-poor environments (Connor, 2005).
Fig. 15.1. Diurnal changes in stomatal conductance (22 September) in the leaves of
the cultivar Koroneiki as a function of the irrigation regime (100, 66 and 33% of
evapotranspiration).
158
Chapter 15
Fig. 15.2. Diurnal changes in water potential in the leaves of the cultivar Koroneiki
as a function of the irrigation regime (100, 66 and 33% of evapotranspiration).
Fig. 15.3. The relationship between photosynthetic rate (Pn) and stomatal
conductance in the cultivars Mastoides and Koroneiki (data from an experiment
with various levels of water application).
The hydraulic resistance of the whole tree, estimated from sap ow and leaf
water potential, revealed changes in the liquid/water path from soil to leaves. Stem
and root hydraulic conductivity in the olive can be reduced under drought stress
due to cavitation. The maximum value of stomatal conductance was recorded
early in the morning and decreased throughout the day, with the lowest values in
159
Fig. 15.4. The relationship between pressure potential (p) and water potential ()
in the cultivars, Mastoides and Koroneiki (data from an experiment with various
levels of water application).
Table 15.5. The osmoregulatory capacity of eight selected Greek olive cultivars
(from Therios, unpublished data).
Cultivar
Manzanillo
Amygdalolio
Frantoio
Adramyttini
Megaritiki
Amphissis
Koroneiki
Osmoregulatory capacity (MPa)

0.80
1.30
0.95
0.30
0.50
0.20
0.15
0.40
the non-irrigated trees. Positive correlation between stomatal conductance and

leaf water potential indicates that olive trees are partially able to restrict water loss
by closing stomata. Furthermore, a close correlation between photosynthesis and
stomatal conductance was recorded (Moriana and Fereres, 2002).
During irrigation of olive orchards it is important to wet the entire area
covered by the root system; patches of non-wetted soil may restrict performance
due to ABA signalling. More productive olive plantations could be achieved on
soils with increased hydraulic conductivity and through appropriate orchard
management (pruning, planting density, etc.).
160
Chapter 15
PRINCIPLES OF IRRIGATION PROGRAMMING

There are various methods for the scheduling of water application in olive
orchards (Fereres and Castel, 1981; Fereres et al., 1981; Beede and Goldhamer,
1994). The most common method is to rely on previous experience and to
monitor soil moisture levels by hand, or with instruments, and to add the
necessary amount of water. The other way is to measure crop water use.
Increasing efforts are being invested in the development of new techniques for
a more precise irrigation of olive orchards. Among those, leaf or stem water
potential, trunk diameter changes, sap ow and infrared thermography are
included. Therefore, the tree is used as a sensor of soil water status.
Measuring soil water

Many instruments measure and soil matric potential. Such measurements
are useful in monitoring soil water status and in programming irrigation.
These sensors are very cheap and may be automated.
Measuring leaf water potential (leaf and stem)

Leaf water potential has been used extensively to monitor tree water status and
to determine the optimal time for irrigation (Sorrentino et al., 1999). In recent
years stem water potential which is less sensitive to variations in atmospheric
conditions has been used extensively. According to Fernndez et al. (1997),
0.46 MPa is a constant average pre-dawn value of leaf, and a leaf value of
0.05 MPa can be considered as a threshold for satisfactory water recovery
overnight. Based on measurements in central Spain, midday stem values of
1.2 MPa for an off year and 1.4 MPa for an on year are recommended as
thresholds for irrigating mature olive orchards. Measurements are labour
intensive, and the threshold values may have to be adjusted, depending on the
orchard.
Measuring variations in trunk diameter

This technique can be used to schedule irrigation when decit irrigation is used
(Goldhamer and Fereres, 2001; Michelakis and Barbopoulou, 2002; Moreno et
al., 2006). The fundamentals of this technique are the shrinkage of the trunk
during stress and expansion during rehydration. This method is suitable for
scheduling irrigation.
161
Measuring sap ow
Transpiration and water uptake by the roots could be estimated from sap ow
measurements (Fernndez et al., 1999, 2001; Giorio and dAndria, 2002;
Tognetti et al., 2004).
Infrared thermography
With this method we can determine the appropriate time for irrigation, but not
the required amount of water. This technique is expensive, requires image
analysis with the appropriate software but is suitable for use in hot and dry
conditions. Furthermore, we can determine variability within the orchard due
to both soil and tree conditions.
THE LOW-VOLUME IRRIGATION TECHNIQUE

In recent years low-volume irrigation (LVI) systems have been introduced into
olive orchards, with favourable results. These involve drip irrigation and minisprinklers, which offer the possibility of applying the irrigation water directly to
the root zone of the olive trees, without wetting the soil between the rows.
Furthermore, the water can be applied frequently at low application rates and
the system allows fertilizer to be incorporated into the irrigation water.
Therefore, the cost of fertilizer application is low and the fertilizer is applied
directly to the active root zone of the trees. The frequent and low-dose
irrigation creates a relatively shallow and brous root system, which is very
efcient with regard to water and nutrient absorption. Therefore, the trees
exhibit a rapid growth rate and produce high yields. On heavy and shallow
soils it is preferable to irrigate with the drip system. However, the irrigation
method depends on soil depth, texture and water quality. In light-textured soils
or when planting at large spacings, trees should be irrigated with jet-stream
sprayers or mini-sprinklers. Spray irrigation is advantageous on sloping areas.
In areas with limited water resources during the vegetative period, extra
irrigation at the critical stages (ower bud differentiation, etc.) makes a
signicant contribution to the annual yield (see Table 15.6).
Irrigation of young olive trees by the low-volume

irrigation technique
In orchards irrigated by the LVI system water is applied every 37 days,
depending on the soil type and environmental conditions affecting the
162
Chapter 15
Table 15.6. The critical periods of water stress for olives, and their effects.
Growth stage
Effect of soil drought
Flower bud development

Flowering
Fruit set
Reduction of ower bud formation

Pistil abortion
Reduction in fruit set
Alternate bearing
Reduced shoot growth
Reduced cell division
Small fruit size
Shrivelling of fruit
Poor shoot growth
Small fruit size
Reduced cell expansion
Shrivelling of fruit
Poor shoot growth
Shoot growth
First stage of fruit growth
Cell division
Cell enlargement
(3rd stage of fruit growth)
evapotranspiration rate. When drip irrigation is applied, two emitters per tree
should be used and, in the case of mini-sprayers, these are positioned upside
down to reduce the wetted radius. Nitrogen application through the irrigation
system increases the vegetative growth of young trees.
As trees grow, their root system grows too, and a wider area of the olive
grove has to be irrigated and the water quantity increased. This can be
accomplished by placing the mini-sprayers in an upright position and, in the
case of drip irrigation, by adding another drip laterally.
IRRIGATION OF MATURE OLIVE TREES

The quantities of irrigation water required by olives are calculated based on
the daily evaporation of the Class A pan. A pan means the evaporation of free
water from a pan that has been used for vegetation by the introduction of
empirical coefcients. The potential ET from an area with a green crop well
supplied with water is assumed to be 0.60.8 of the free water evaporation
depending on season, area, length of daylight, etc. These values serve as a
basis for determining olive grove ET by multiplying the evaporation from the
Class A pan by the appropriate crop coefcient. This coefcient depends on
the stage of growth and whether the fruit is intended for olive oil or table
olives.
163
THE PARTIAL ROOT ZONE-DRYING IRRIGATION

TECHNIQUE
General
The root system of plants in contact with drying soil is known to produce chemical
signals that travel via the xylem and effect a restriction in stomatal aperture and
leaf expansion rate. Therefore, modern irrigation systems exploit this chemical
signalling capability in reducing transpirational water loss and crop water
requirement (Goldhamer et al., 1994; Centritto et al., 2005). In the early 1990s
the use of a split-root system was established. In this system the roots of
experimental plants were divided between two pots; one part of the root system
was irrigated in order to deliver sufcient water to the shoots and to sustain
photosynthesis and water relations, while the rest of the root system was
maintained in dry soil. The principle of the split-root technique is to minimize the
negative effects of decit irrigation, by sustaining shoot water equilibrium. The
split-root technique was expanded into a eld-based decit irrigation system called
partial root zone drying (PRD); this reects the alternate drying and re-wetting of
the two halves of the root system in order to sustain water relations and yet to
generate root signals that limit transpirational water loss. This is accomplished by
allowing part of the root system periodically to dry the soil in which it is rooted.
With this method, transpiration is restricted; leaf area is also restricted
while no negative effects on yield were recorded. Furthermore, decrease of the
vegetative vigour improved the olive quality. Also, water use efciency (WUE)
was improved, meaning a decrease in the required amount of water, which
today is scarce and very expensive.
Effects of partial root zone drying on olive oil quality

The oil content and acidity of olive fruit showed no signicant differences
between partial root zone drying (PRD) treatment and full irrigation. The PRD
irrigation strategy, which restricts water irrigation supply, reduces the quantity
of the salt incorporated within the root zone and permits the sustainability of
the agricultural system.
Very few studies have been conducted on the effect of saline water and PRD
strategy on oil quality (Klein et al., 1994). PRD treatment leads to an increase
in oleic acid content, in the same way as rainfed conditions.
Decit irrigation
Due to irrigation water scarcity, growers must apply less water, i.e. follow a
decit irrigation approach that provides less than the olive tree needs, in such a
164
Chapter 15
way as to achieve close to maximal production (Goldhamer et al., 1994). Three

systems are described:
1. Sustained decit irrigation (SDI). The grower applies a reduced percentage
of ETc during the whole irrigation period.
2. Low-frequency decit irrigation (LFDI). The soil is left to become dry, since
all the available water has been absorbed. Consequently, the soil is irrigated to
eld capacity and again is left to dry.
3. Regulated decit irrigation (RDI). This method supplies up to 100% of ETc
during the stage that the crop is sensitive to water stress and reduced water (by
3040%) during the remaining growth period. Hence, the required water for
irrigation is about 50% of ETc (Fernndez et al., 2006). This method has, as a
prerequisite, a thorough knowledge of the physiology of the crop.
THE EFFECT OF IRRIGATION ON THE QUALITY OF TABLE

OLIVES AND OLIVE OIL
Irrigation induces greater shoot growth and increased total leaf surface area,
photosynthesis and transpiration. Irrigation does not inuence fruit shape but
increases fruit weight, volume and pulp:pit ratio. The larger fruit size is
primarily the result of both a larger number of cells and the positive effect of
water availability on cell division rather than cell expansion. Irrigation has no
substantial delaying effect on ripening. With irrigation, pulp water content
increases and rmness decreases slightly. Irrigation results in less sugar
content, which can be unfavourable to fermentation. Therefore, it could be
useful to add sugars to the brine to produce good lactic fermentation, which is
important for good fruit storage.
Olive oils produced without extra irrigation and based completely on
annual precipitation have higher polyphenol contents (Motilva et al., 2002),
bitter index and stability. Furthermore, those from non-irrigated orchards have
higher levels of oleic and linoleic acids (Castro et al., 2006).
The quantity of the water applied to trees exerts a profound inuence on
olive oil quality (Patumi et al., 1999; DAndria et al., 2002; Faci et al., 2002;
Motilva et al., 2002; Berenguer et al., 2004, 2006; Grattan et al., 2006;
Herenguer et al., 2006). Producers who wish to optimize oil production
which is a function of both yield and percentage oil content need to take into
account the benets of moderate irrigation, since excessive irrigation
drastically reduces oil yield. Moderate water stress gives a better quality of olive
oil, i.e. greater polyphenol content, greater stability and better avour;
irrigation increases productivity but has a negative effect on oil quality. The
total polyphenol content (K270, K232) and oxidative stability (K225) are
dramatically affected by the level of irrigation. The more water applied the
more likely it is that the oil will have a signicant reduction in both polyphenol
165
content and stability; since polyphenols are water soluble the water content of
the fruit can inuence the amount of polyphenols remaining in the oil after
processing. Increasing the degree of irrigation may affect oil avour, becoming
bland. Minimally to moderately irrigated trees produce oils that are fruitier,
with a balanced ratio of bitterness and pungency (see Table 15.7).
If a producer wants to increase the intensity of bitterness and pungency
they should limit water application. An irrigation system supplying 40% of ETc
gives good oil extractability and maintains excellent oil chemical parameters.
IRRIGATION BY WASTE WATER

The scarcity of water and the expected decrease of precipitation in coming
years will create problems for olive grove culture. In this respect, many
countries are devising ways of using waste water for irrigation, since waste
water is an additional alternative and valuable source for coping with the
scarcity of irrigation water. However, re-use of waste water for irrigation may
involve risks for both environmental and human health. Therefore, waste
Table 15.7. Chemical analysis of olive oil (2003) from the cv. Picual under four
different irrigation treatments (Irrigation Maximum, Imax; Progressive Decit
Irrigation, D1; Linear Decit Irrigation, D2; and Rainfed, R (from Castro et al., 2006).
Chemical
-Tocopherol (mg/kg)
-Tocopherol (mg/kg)
-Tocopherol (mg/kg)
Total tocopherols (mg/kg)
Polyphenols (mg/kg)
Bitter index (K225)
Stability (h)
Carotenoids (mg/kg)
Chlorophylls (mg/kg)
C16 (%)
C16:1 (%)
C17 (%)
C17:1 (%)
C18 (%)
C18:1 (%)
C18:2 (%)
C18:3 (%)
C20 (%)
C20:1 (%)
C20:2 (%)
*P
Imax
D1
D2
220.6
2.4
11.4
234.4
359.6
0.32
156.70
4.28
5.26
13.80
1.12
0.07
0.13
3.40
77.0
2.99
0.61
0.39
0.30
0.17
230.2
1.4
10.6
242.2
447.0
0.29
149.90
3.74
7.96
13.11
1.16
0.05
0.11
3.25
77.9
2.98
0.64
0.43
0.27
0.09
225.0
2.2
11.2
238.4
542.6
0.37
169.98
4.40
4.44
12.65
1.04
0.07
0.11
3.31
78.7
2.74
0.59
0.40
0.28
0.13
0.05; **P 0.01; ***P 0.001.
R
210.0
2.4
8.6
221.0
1183.8
0.57
228.49
5.24
5.64
11.36
1.03
0.06
0.09
3.48
79.0
3.52
0.67
0.45
0.23
0.10
Signicance
***
***
***
**
*
**
**
**
*
*
166
Chapter 15
water should be appropriately treated before use, for both health protection
and avoidance of crop damage. Only in the USA, Australia and South Africa
have any specic regulations been established for re-use of waste water. In
several EU countries regulations and codes of practices concerning the use of
treated waste water for irrigation are under preparation or revision (Angelakis
et al., 2002). Irrigation with waste water is now a common practice in many
countries.
The nutrient content of waste water is benecial for olives. However, high
nutrient concentrations may constitute a source of pollution or may be toxic to
plants. This may cause underground or surface water pollution, excessive
vegetative growth and reduction in quality.
The nutrients in waste water essential for plants are nitrogen, phosphorus,
potassium, zinc, boron and sulphur. The N content of municipal waste water
after secondary treatment ranges from 20 to 60 mg/l. Therefore, the N in
treated waste water may be in excess of crop needs. Phosphorus levels in
secondary treated waste water vary from 6 to 15 mg/l, and those of K range
from 10 to 30 mg/l; with regard to other nutrients, waste water contains
adequate amounts of S, Zn, Cu and B. Treated waste water contains enough B
for plant nutrition and, in some cases, B is often found in excess, causing
toxicity. The most toxic ions in treated waste water are Na, Cl and B. Olive is
tolerant to waste water containing B at a level of up to 1 mg/l. The greatest
concern with regard to the re-use of waste water, however, is the presence of
pathogens.
16
WATER USE EFFICIENCY BY THE OLIVE
INTRODUCTION
The olive tree is very efcient in its use of water. As an illustration, it requires
312 g water to produce 1 g dry matter, whereas other evergreen plants such as
Citrus and Prunus species require 400 and 500 g, respectively. Water
consumption by the olive is 30 and 40% of that of Citrus and Prunus species,
respectively. The mechanisms conferring high water use efciency (WUE)
include the ability of the olive leaves to tolerate very low values (Connor,
2005) and osmotic adjustment via the synthesis of osmoregulatory solutes
(Morgan, 1984). Furthermore, the use of growth retardants increases the
drought resistance of olive plants (Frakulli and Voyiatzis, 1999).
WHAT IS WATER USE EFFICIENCY?

The ratio between the amount of water transpired by plants and dry matter
accumulation is known by various terms such as transpiration coefcient,
transpiration ratio or water requirement of plants, while the reciprocal is
known as the efciency of transpiration. Nowadays the term WUE is used
most frequently (Bacon, 2004).
Terrestrial plants have evolved mechanisms that enable control of water
loss, without any decrease in photosynthesis. This is very important, since the
available water is the determining limiting factor for agricultural productivity.
These mechanisms maintain survival of plants and productivity under limited
water supply and include the following:
1. Mechanisms of avoidance of water decits:
Short growth cycle; the plant completes its life cycle before the appearance
of any water decit.
Limited leaf area (small leaves), closure of stomata and thick cuticle.
167
168
Chapter 16
2. Mechanisms of tolerance to water stress:
Osmoregulation, elastic modulus of elasticity and turgor maintenance.

Synthesis of organic solutes to maintain turgor (sucrose, mannitol,
proline, etc.) and water stress-tolerant proteins and enzymes.
3. Efcient use of irrigation water:
Genotypes with high WUE.

Appropriate irrigation systems and methods of achieving efcient water
use (see Table 16.1) (Vermeiren and Jobling, 1980; Motilva et al., 2000;
Fernndez et al., 2003, 2006).
Various denitions of WUE are to be found in the literature (Centrito et al.,

2002):
1. WUE is the ratio of the produced biomass to plant transpiration.
2. WUEt; this is known as the transpiration efciency. The WUE can also be
expressed as the ratio of total biomass, shoot biomass or yield harvested to total
ET or plant transpiration (Ep). Furthermore, WUE could be expressed in terms
of dry mass, fresh mass or glucose equivalent of those masses.
3. WUEe is the ratio of net CO2 assimilation rate to transpiration. The values of
WUE, which are based on gas exchange, can be converted to WUE based on dry
matter, and the factor 0.610.68 kg dry matter/kg CO2 xed. The value of WUE
at a given site is stable for a range of cultivars or similar species. The WUE for a
wide range of C4 plants ranges from 2.41103 to 3.88103. These values are
signicantly greater than the values for C3 plants (0.881032.65103).
Furthermore, Crassulacean acid metabolism (CAM) plants exhibit up to ten
times the values recorded for C4 plants (2010335103).
The equations describing net assimilation rate, transpiration and WUE are
presented (Jones, 2004):
Net assimilation rate (A) =
papi
Pa (ra + rs + rm)
Table 16.1. Efciency of various irrigation systems.

Irrigation system
Efciency (%)
Drip-microsprinkler
New sprinkler
Older sprinkler
Basin
Contour ood
Furrow
9095
7585
6580
7580
6065
4060
Water Use Efciency
169
where pa = atmospheric partial pressure; pi = internal partial pressure of

CO2 in leaves; Pa = atmospheric pressure; ra, rs and rm = the resistances
(boundary layer, stomatal resistance, mesophyll resistance).
The equation for transpiration is Ee =
el ea
Pa (ra + rs)
where el, ea = saturated water vapour pressure of leaves and air,

respectively.
After combining these two equations the following ratio is obtained (Jones,
2004):
A
(pa pl)
(el ea)
WUE =
(ra + rs)
(ra + rs + rm)
This ratio indicates that WUE is expected to increase as stomata are

closing.
The measurement of WUE is based on measurements with the carbon
isotope 13C. The principle of this measurement is based on the discrimination
by photosynthesis between 12C and 13C. Therefore, less 13C is expected to be
found in the dry matter than in the air. This discrimination is usually expressed
as and is described by the symbol .
=
(13C/12C) air
(13C/12C) dry matter
The value ranges between 13 and 28 for C3 plants and between 1 and
7 for C4 plants. Another way of expressing is:
= 0.0044 + 0.0256 pi/pa
where pi = internal space partial pressure of CO2; pa = atmospheric partial
pressure of CO2.
PLANT NUTRITION AND WATER USE EFFICIENCY

The response to less than optimum nutrient supplies leads to growth rates below
maximum and reduces WUE. There is also a parallel between WUE and relative
growth rate, with increased WUE at higher growth rates. With regard to sources
of N, lowest water use efciency is for N2 in symbioses relative to NO3 + NH4 N
sources. Of all N sources ammonium gives lower water use efciencies than does
NO3. Nutrients can inuence the development of stomata, altering stomatal
density and hence potential maximum leaf conductance.
In k-decient plants stomatal density is lower, leading to transpiration
decrease. Calcium is another ion that functions in the regulation of stomatal
170
Chapter 16
aperture, due to its concentration in the apoplast of the leaf epidermis. In the
case of calcifuges, high levels of Ca2+ in the rhizosphere could reduce the
coupling of stomatal conductance to assimilation, and this reduces water use
efciency by increasing apoplasmic Ca2+ in the leaf. Nitrogen sources could
alter stomatal conductance by activating nitrate entry into guard cells.
Another inorganic anion involved in stomatal opening is Cl. Therefore, the
stomatal conductance of NH4+-fed plants is 2040% lower than that of those
fed on NO3.
Deciency of certain elements is accompanied by organic carbon efux, as
soluble, low-relative molecular mass compounds (Raven et al., 2004). This is
especially true for deciency of phosphorus and Fe. This mechanism is
signicant.
Water use efciency can be increased by following more intensive cropping
systems in semi-arid environments and by increasing plant population density
in more humid environments. Important factors determining water and
nutrient use by crops include the physical and chemical properties of soil (water
retention, capacity, available water, microbial biomass, soil organic matter).
Certain practices increase nutrient use by plants and include the form and
quality of added fertilizer and the timing of its application. In order to maximize
the use of applied fertilizer, precision farming tools such as GPS digital maps of
soils and crops, and also the use of optical sensors and growth models, have
been applied.
METHODS OF IMPROVING WATER USE EFFICIENCY

Introduction
More than 75% of fresh and good-quality water used throughout the world is
destined for agriculture. Today more than 3100 km3 of water is used in
agriculture for the irrigation of approximately 240 million ha of crops. The
sources of irrigation water are either surface or groundwater. Due to overuse of
irrigation water a signicant reduction in groundwater reserves has been
recorded in many agricultural areas. Therefore, it is necessary to improve water
use efciency (Fernndez and Moreno, 1999), by which we mean the amount of
water absorbed necessary to produce 1 unit of biomass or harvested crop.
Regulated Decit Irrigation (RDI) and Partial Rootzone

Drying (PRD) irrigation
Crop performance is affected by many factors such as cultivar genotype,
humidity, precipitation and wind, pruning, nutrition and irrigation. Of these
Water Use Efciency
171
factors irrigation is the most important, and growers are now exploring new
ways of manipulating the water use efciency of crops by using new irrigation
techniques.
One such technique is Regulated Decit Irrigation (RDI) (Loveys et al.,
2004; Tognetti et al., 2005). This is an irrigation technique based on the
science of phenology regarding vegetative and reproductive development.
Plant vigour is very important for fruiting, and excessive vigour results in
shading and decreased fruiting. Reduced vigour can be achieved through
pruning, application of growth inhibitors (Frakulli and Voyiatzis, 1999) or
appropriate fertilization and irrigation. By judicious use of these methods,
manipulation of vigour through irrigation management offers more exibility,
with minimal labour input (Dichio et al., 1994; Centritto et al., 2005). The
main principle of RDI is that the quantity of applied water is reduced during the
period of high vegetative growth and low fruit growth rate. Later, when fruit
growth rate is high, a normal irrigation regime is resumed. Due to water decit
the vegetative growth is reduced while fruit growth and development is least
affected. Due to water decit the canopy size and leaf stomatal conductance are
reduced and intermittent drought affects leaf anatomy (Chartzoulakis et al.,
1999; Dichio et al., 2003). RDI is a method developed for high-density
orchards. Although the root system remained dry, the parameters of
photosynthesis, stomatal conductance and growth regained pre-stress values
within a few weeks, although one part of the root system still remained dry
(Dry and Loveys, 2000).
Stomatal closure is due to the production of ABA in the dry part of the root
system (Stoll et al., 2000). These responses were not evident in plants where
the same half of the root system was irrigated throughout the growing season
and the remainder of the roots were dry. This suggests that the root-derived
signals during the stomatal responses were not sustained, in the same way that
had been observed in various plants. Furthermore, PRD did not result in great
changes in leaf ABA in comparison with plants subjected to an overall water
decit (Stoll et al., 2000). We may assume that stomatal control is achieved
with small alterations in leaf ABA, when ABA synthesis is initiated by changes
in water availability to only part of the root system. Measurement of ABA
content after PRD indicates that its content is almost equal after extraction
from roots and petioles, and twice as great from leaf blades.
The pH of the sap from PRD-treated plants was higher in comparison with
controls. This increase in the pH of xylem sap reduces partitioning of ABA into
the symplasm, i.e. away from the sites of action of guard cells. The ABA
concentration of xylem sap varies, depending on the cultivar and species.
Furthermore, the pH of xylem sap varies depending on plant species (Wilkinson,
2004). When the pH of the xylem sap is close to the pKa value of ABA (4.8), very
small changes in sap pH will create large changes in the proportion of ABA
present as an ion, while greater pH values have less effect on ABA ionization.
Under high VPD stomatal conductance was low in both control and PRD trees
172
Chapter 16
on the western side of the trees. Furthermore, the difference in stomatal

conductance between control and PRD trees was evident in the morning, on the
side of the tree receiving less direct sunlight.
In response to water stress roots synthesize the ethylene precursor
1-aminocyclopropane-1-carboxylic acid (ACC) (Wilkinson, 2004). This is
transported to shoots, where ethylene, released from ACC, can induce ABA
synthesis. PRD results in stomatal closure, and exogenous application of the
cytokinin benzyladenine can overcome the inhibitory effect. Furthermore,
PRD reduces endogenous concentration of zeatin and zeatin riboside in both
roots and shoots.
Partial root drying is an effective technique for improving WUE, by changing
the relationship between stomatal conductance and ambient evaporative
condition. Furthermore, PRD increases root growth in deeper soil layers. This
inuences the WUE, since more water is available from greater soil depths. PRD is
effective in crops which, after stress, produce ABA to elicit a stomatal response. In
this case another mechanism is active, which is the accumulation of osmotically
active solutes maintaining stomatal conductance, even at low leaf water
potentials. This is achieved through a decrease in osmotic potential and the
maintenance of turgor at low water potentials.
PRD as a system for irrigation requires that the irrigation system is
installed in such a way that 50% of the root system is irrigated and 50% is dry,
in any given period. Above ground drip-pipes are used.
WATER USE EFFICIENCY AND CHEMICAL SIGNALLING

Plants require CO2 via open stomatal pores on the leaf blade and, at the same
time, they also risk dehydration via water loss through transpiration. Therefore,
plants lose water in order to x CO2. To enable that, plants have been adapted to
allow control of water loss and this is achieved by controlling stomatal aperture,
through pairs of guard cells. The effect of stomatal closure in improving WUE
occurs during the rst stages of water stress. However, during severe stress
photosynthesis is severely inhibited and WUE decreases again. At leaf level the
plant achieves water balance by leaf rolling, leaf epinasty, denser leaf trichomes
and a thicker cuticle, reduced leaf area and shedding of older leaves. This
reduces the surface area from which water can be lost and redistributes
nutrients to the stem, roots, fruits and leaves. Another response is the increase
in the root:shoot ratio. Under water stress shoot growth is reduced while root
growth is increased. This allows the plant to scavenge moisture from a larger
volume of soil, while minimization of water loss from the aerial part of stomatal
closure and leaf growth inhibition is among the earliest responses to drought,
and can occur after very slight soil drying.
Many plants will regulate stomatal aperture independently of hydraulic
signals but in response to chemical signals. These chemical signals are
Water Use Efciency
173
generated by the interaction between the root and the drying soil, controlling
WUE as water decit develops in the soil. Chemical signals involve changes in
the transport of growth regulators or other substances via the xylem, as
described below.
ABA is synthesized in the root, as soil drying progresses, and is subsequently transported to the leaf, via the xylem vessels (Wilkinson, 2004). In
leaves, ABA accumulates in the guard cells and induces stomata to close. ABA
accumulation induces a signal transduction inside the guard cell, which
involves an increase in cytoplasmic Ca. This Ca increase, and also an increase
in cytoplasmic pH, depolarize the guard cell plasma membrane causing
outward K+ and Cl channels to open, followed by efux of these ions. This
leads to decreased water potential of the cell, reduced turgor and stomatal
closure. The synthesis and transport of ABA take place before a signicant
decrease in soil or leaf water, i.e. before the appearance of hydraulic signals.
Other chemical signalling molecules include cytokinins, ethylene, NO3 and pH.
Soil drying reduces transport of growth-promoting cytokinin from the root to
the shoot.
The basic ABA signal can also be inuenced by soil drying via synthesis of
ABA conjugates such as ABA-glucose ester (ABA-GE) and their transport via the
xylem to leaves. ABA-GE is lipophilic and unable to cross lipid cell membranes,
and is transported to the shoot with no loss to stem parenchyma. Therefore ABAGE is a more efcient form of transporting ABA from the root to the shoot. When
ABA-GE reaches the leaf apoplast, enzymes such as esterases, hydrolases and/or
glucosidases cleave the conjugate and release free ABA to the cells.
Furthermore, soil drying and ooding increase ethylene levels by promoting
the synthesis of the ethylene precursor ACC and its transport from roots to
leaves. Water stress, soil ooding and nutrient deciencies can induce changes in
the xylem sap pH. The rst change measured is an increase in xylem pH after 24
h of exposure to soil drying and prior to the reduction in stomatal aperture.
Ethylene generation by leaves occurred after 34 days. Furthermore, xylem sap
pH becomes more alkaline after soil ooding. Such changes in pH can induce
stomatal closure by affecting ABA compartmentation. Increases of apoplastic sap
pH from 5.5 to approximately 7.0 in water-stressed plants have been reported.
The same trend regarding pH was exhibited by nitrate-decient soil. Changes in
xylem and symplastic pH may be linked to effects of cellular dehydration or the
activity of H+-ATPases associated with xylem parenchyma. Furthermore, soil
drying-induced changes in ionic composition of xylem sap consequently affect its
pH. Reduction of NO3 in the xylem sap is a sensitive chemical change that
precedes increases in xylem sap pH and stomatal closure. Soil drying induces
xylem alkalinization, which may result in a decrease in nitrate reductase (NR)
activity from roots to leaves.
This can be promoted by a reduced supply of nitrate from the root to the
leaf. Malate and citrate are loaded into the xylem instead of NO3, which
increases pH. This happens in species which transport NO3 to the leaves and
174
Chapter 16
assimilate it there. Species with NR located in the root transport organic

products of root NO3 assimilation via the xylem, and sap pH will remain stable
during soil drying. This is common with deciduous woody species.
Another chemical signal involves cytokinins. Root apical meristems are a
major site of synthesis of free cytokinins. Cytokinins are transported through
the xylem to the shoot as zeatin riboside and are released as free zeatin in the
leaves. Cytokinin is synthesized and transported to the shoot at a rapid rate in
non-water-stressed plants. Upon drying of the soil the cytokinin content of
xylem sap is reduced due to reduced synthesis. This reduction sends a negative
message to the shoot to reduce growth and stomatal opening. High cytokinin
concentrations induce increased k concentrations within guard cells and
increase their turgor.
17
STRESS-INDUCED ACCUMULATION
OF PROLINE AND MANNITOL
INTRODUCTION
Accumulation of proline has been proposed as a protector of membranes,
polyribosomes and enzymes during water or salt stress. Proline is an
osmoregulant and contributes to 45% of total osmotic adjustment, and can also
detoxify free radicals. Most proline is accumulated in the vacuoles, but some is in
the cytoplasm.
Proline is accumulated after stress by both increased synthesis and
reduced degradation (Van Rensburg et al., 1993; Hare and Cress, 1997;
Yoshida et al., 1997). Proline may be synthesized from both glutamate and
ornithine. The intermediates in proline synthesis are glutamic -semialdehyde
(GSA) and -pyrroline-5-carboxylate (PCP). The enzymes involved in
synthesis are P5C reductase and proline dehydrogenase.
COMPATIBLE SOLUTES
Olives are grown mostly in arid and semi-arid regions, where they are subjected
to high temperatures and scarcity of water. Osmotic homeostasis requires
an increase of osmotic pressure in cells by either uptake of soil salts or the
synthesis of metabolically compatible compounds. These solutes are commonly
carbohydrates (sugar or sugar alcohols, such as mannitol), amino acids and
quaternary ammonium compounds (Hare and Cress, 1997; Hare et al., 1999).
Besides their role in osmotic adjustment (Hare et al., 1998), compatible solutes
also have osmoprotective functions protecting membranes thus preserving
the biological functions of these, and seem to play a role in hydroxyl radical
scavenging, hence being active in protecting plants against oxidative damage
(Smirnoff and Cumbes, 1989).
Sucrose is the most abundant carbohydrate and is an important factor in
micromolecule and membrane stabilization. Sugar alcohols too, such as
mannitol (Bieleski, 1982), constitute an important group of compatible solutes.
175
176
Chapter 17
Mannitol is an osmoprotectant and also serves as a storage compound and a

redox agent. Mannitol degradation produces, in its initial stage, NADH and its
oxidation in mitochondria yields up to three molecules of ATP (Stoop et al.,
1996). Fructose represents those reducing sugars frequently involved in
osmotic adjustment in stressed cells.
Proline is one of the most common compatible solutes accumulated in
plants under stress conditions (Yoshida et al., 1997). It contributes to osmotic
adjustment, preserves enzymes and other important cellular structures and is
a reserve for carbon and nitrogen following stress relief. Increasing osmotic
stress stimulates the accumulation of mannitol as a compatible protective
compound in olive.
The contribution of the ornithine pathway to salt-induced proline
synthesis is less important. The oxidation of proline to glutamate is restricted to
the mitochondria. Proline accumulation is an excellent means of storing
energy, since the oxidation of one molecule of proline can yield 30 ATP
equivalents.
Six enzymes participate in proline biosynthesis and degradation (Kavi
Kishor et al., 1995; Peng et al., 1996; Savour et al., 1997; Hare et al., 1999):
PCP synthetase.
PCP reductase.
Proline dehydrogenase.
PCP dehydrogenase.
Ornithine -aminotransferase.
Arginase.
The reactions involving these enzymes are presented in Table 17.1.
Table 17.1. Enzymatic reactions of proline biosynthesis and degradation in plants.

Enzyme
Reaction(s) catalysed
1-pyrroline-5-carboxylate synthetase
(i) Glutamate + ATP Glutamyl--phosphate + ADP

(ii) Glutamyl--phosphate + NADPH+H+ glutamic-semialdehyde
1-pyrroline-5-carboxylate + NAD(P)H+H+
proline + NAD(P)+
Proline + O2 + FAD 1-pyrroline-5-carboxylate
+ H2O + FADH2
1-pyrroline-5-carboxylate + NAD(P)+ glutamate
+ NAD(P)H + H+
Ornithine + 2-oxoglutarate glutamic--semialdehyde
1-pyrroline-5-carboxylate + glutamate
Arginine + H2O ornithine + urea
1-pyrroline-5-carboxylate reductase
Proline dehydrogenase
1-pyrroline-5-carboxylate dehydrogenase
Ornithine -aminotransferase
Arginase
Stress-induced Proline and Mannitol
177
MANNITOL CONTENT OF OLIVES

Mannitol is a widespread polyol throughout the plant kingdom, occurring in
more than 100 species of vascular plants. Mannitol may participate in many
physiological processes in plants, such as: (i) carbon storage and translocation
of products (Flora and Madore, 1993; Conde et al., 2007); (ii) regulation of
reductive power by production of NADPH; (iii) osmoregulation and regulation
of H2O status (Peltier et al., 1997); and (iv) a hydroxyl radical scavenger and a
cryoprotectant. In the family Oleaceae mannitol represents a signicant
proportion of the carbohydrate pool. Mannitol content increases in response to
conditions of increased drought or salinity (Peltier et al., 1997).
The mannitol content of the leaves of olive plants under salinity stress
increased from 160 to about 220 mol/g d.w. (Tattini et al., 1996), and
mannitol represents 70% of the soluble carbohydrates in olive leaves. The
mannitol level in olives is more or less constant during the year (Oddo et al.,
2002). The poor correlation (Oddo et al., 2002) between mannitol content in
Olea and rainfall or temperature suggests that mannitol does not play a
fundamental role in the response to drought or high temperature.
18
MINERAL NUTRITION OF THE OLIVE
CHAPTER OVERVIEW
Olive trees in many areas of the world are among the least fertilized trees.
Therefore, biennial bearing is very common. Although olive trees represent a
large global hectareage, our knowledge of their mineral nutrition and salt
tolerance is very limited. Hence, this chapter represents an attempt to provide
some information in this area.
The topics discussed in this chapter are the following:
The needs of olives for N, P, K and their concentration in various plant parts.
The annual needs for N, P, K and Ca for various fruits and the annual
variations in N, P, K, Ca and Mg concentrations in leaves. Nitrogen
distribution in fruiting olive shoots.
The concentrations of various nutrients considered as being adequate.
Nutrient stress and growth.
Nutrient deciency or excess and photosynthesis.
Nitrogen excess and quality of olive oil and table olives.
Roles of N and its assimilation, kinetics of nitrate (NO3) absorption and
effect of N form on growth.
Olive culture and the nitrate accumulation problem.
Nitrates leaching from olive orchards.
Roles of K, P, Ca and Mg and their absorption.
Roles of B and Mn.
Response of olive trees to salinity.
Olive fertilization and slow-release fertilizers.
INTRODUCTION
Olive trees are not big feeders. They are hardy plants that will tolerate poor
growing conditions especially low fertility better than almost any other
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Chapter 18
fruit tree. They also tolerate a very wide range of soil pH and tend to fruit better
under conditions of average vigour and nutrition. The olive is a tree that, in
general, receives minimal fertilization. This unfavourable treatment of olives
concerning the application of organic or inorganic fertilizers derives from the
opinion that they do not require adequate fertilization, because of their
extensive root system. This opinion is misdirected, since the volume of soil
around the root system is limited. As a consequence of such inadequate
fertilization, biennial bearing is quite frequent. There is a lack of information in
international literature concerning the behaviour of olive cultivars with regard
to mineral nutrients (Marschner, 1997). Therefore this chapter aims at giving
information pertinent to olives in respect of nutrition. A signicant portion of
these data are derived from research projects of the author or PhD theses
conducted under his guidance.
THE MINERAL REQUIREMENTS OF OLIVE TREES

The needs of olive trees with respect to N, P, K are comparable to the needs of other
fruit trees such as deciduous trees (Gavalas, 1978). The quantities of nutrient
absorbed from orchard soils are 0.50 kg N/100 kg of fruit and 1.0 and 0.76 kg
N/100 kg of leaves and wood, respectively. For K the required quantities are 0.95,
0.56 and 0.39 kg, respectively. The large fruit load, the signicant amount of
leaves and wood removed every year by pruning and the leaf drop due to diseases
and water stress entail, as a consequence, the loss of a large amount of nutrients
from trees. Therefore, it is of vital importance that the lost nutrients are replaced
through appropriate fertilization. For an orchard of 1000 m2 the nutrients
absorbed by olives per annum amount to 1.53.5 kg N, 0.8 kg P, 15 kg K and
25 kg Ca. The nutrients (N, P, K and Ca) required for 100 kg of fruits, 50 kg of
leaves and 50 kg of wood are given in Table 18.1.
Concentrations within the various plant parts of N, P and K are presented
in Table 18.2. Nitrogen concentration decreases in young leaves and stems in
spring and summer and increases during the autumn in both off and on
years. Nitrogen concentration in old leaves and stems remains almost constant
during the off years and is mobilized during the on years to support growth.
Table 18.1. The N, P, K and Ca (kg) required every year by an olive orchard for the
production of fruit, leaves and wood.
Nutrient
N
P
K
Ca
Fruit (100 kg)
Leaves (50 kg)
Wood (50 kg)
Total (kg)
0.500
0.120
0.950
0.960
0.500
0.120
0.280
0.500
0.380
0.150
0.195
0.300
1.380
0.390
1.425
1.760
Mineral Nutrition
181
Table 18.2. Concentration of N, P and K within the various plant components of

olive trees.
Component
N (%)
P (%)
K (%)
Ratio (N:P:K)
Secondary roots
Primary roots
Trunk
Main branches
Secondary branches
Shoots
Leaves, 1 year old
Leaves, 2 years old
Unripe fruits
Ripe fruits
0.33
0.37
0.26
0.29
0.23
0.64
1.63
1.24
0.90
0.97
0.113
0.123
0.070
0.090
0.099
0.179
0.271
0.201
0.333
0.397
0.402
0.477
0.219
0.354
0.191
1.000
0.994
0.678
2.760
3.220
2.9:1:3.5
3.0:1:3.8
3.7:1:3.1
3.2:1:3.9
1.2:1:1.9
3.5:1:5.5
6.0:1:3.6
6.1:1:3.4
2.7:1:8.3
2.4:1:8.1
Leaves have a larger concentration of N than stems, and fruits produced during
the on years are the main sink for N. Furthermore, N also may be mobilized
from organs other than leaves to support fruit growth.
METHODS OF SOLVING NUTRITIONAL PROBLEMS

For determination of the various nutritional problems, three methods of
application are available: leaf analysis, soil analysis and visual symptoms.
Leaf analysis
The nutrient concentrations within olive leaves depend on the cultivar (Jordao et
al., 1999), the level of fertilization and area. In the literature are to be found leaf
analysis data from various regions of the world (Fernndez-Escobar et al., 1994;
Dimassi et al., 1999). The season of leaf sampling is that when the nutrients
attained have a more or less constant concentration. Starting early in spring, the
concentrations of N, P, K and Mg (in leaves) are more or less constant until a drop
in August. Subsequently the concentration increases again and stabilizes from
October to the start of the next vegetative cycle (see Fig. 18.1). The same trend is
observed in leaves of 1, 2 or 3 years old. The winter period is characterized by
stable concentrations of N, P, K, Ca and Mg, and this period is considered as the
optimum for leaf analysis in the olives. However, other workers prefer to collect
leaf samples in July from the current years growth. The required number of
leaves per sample is 80100, and these leaves are harvested from shoots with no
fruits. The nutrient concentrations determining deciency or sufciency of
nutrients are presented in Table 18.3. The chemical composition of olive leaves
varies depending on the cultivar and time of sampling (Table 18.4).
182
Chapter 18
Fig. 18.1. Seasonal variation in N concentration of olive leaves 1, 2 and 3 years old
(from Bouat, 1961).
Table 18.3. The nutrient concentrations in leaves determining deciency,
sufciency or excess for olives (from Therios, 2005b).
Nutrient
N (%)
P (%)
K (%)
Mg (%)
Ca (%)
S (%)
Cl (%)
Fe (ppm)
Mn (ppm)
B (ppm)
Zn (ppm)
Cu (ppm)
Mo (ppm)
Deciency
Relative lack
Sufciency
Excess
< 1.20
< 0.07
< 0.50
< 0.07
< 0.50
< 0.05
1.201.60
0.070.09
0.500.70
0.070.10
0.501.00
0.050.10
2050
520
1520
510
<5
< 0.03
1.601.80
0.090.11
0.700.90
0.100.30
1.002.50
0.100.25
0.100.40
50150
50150
2050
1030
520
> 2.20
> 0.14
> 1.10
> 0.30
> 0.80
NUTRIENT ELEMENTS
Nitrogen
Roles of nitrogen
Nitrogen is necessary for plants since it is a primary component of nucleic
acids, proteins, amino acids, purines, pyrimidines and chlorophyll. Nitrogen
exerts a signicant effect on plant growth, as it reduces biennial bearing and
Mineral Nutrition
183
Table 18.4. Variation in chemical composition of cvs Chondrolia Chalkidikis and

Amphissis leaves 30120 days after full bloom (from Chatzissavvidis et al., 2004).
Nutrient
N (% d.m.)
P (% d.m.)
K (% d.m.)
Mg (% d.m.)
B (ppm)
Fe (ppm)
Days after
full bloom
Chondrolia
Chalkidikis
Amphissis
30
60
90
120
30
60
90
120
30
60
90
120
30
60
90
120
30
60
90
120
30
60
90
120
1.22
0.73
0.83
0.90
0.15
0.11
0.10
0.11
0.94
0.89
1.71
1.63
0.09
0.06
0.07
0.06
47
47
96
122
56
84
18
17
0.93
0.78
0.76
0.65
0.11
0.08
0.11
0.06
0.65
0.73
1.18
1.32
0.06
0.06
0.05
0.05
52
61
84
44
58
97
27
29
increases the percentage of perfect owers. The olive is a fruit tree requiring
enough N and irrigation in order to avoid biennial bearing. Therios and
Sakellariadis (1988) and Fernndez-Escobar et al. (2004a) found that the
increase of N supply increased olive growth. Lack of N leads to decreased
growth, shorter length of annual shoots (< 10 cm), fewer leaves, reduced
owering and decreased yield. Nitrogen concentrations in the leaf of < 1% lead
to the formation of imperfect owers. However, excess N application creates
problems, while the application of slowly available fertilizers has benecial
effects (Fernndez-Escobar and Marn, 1999; Garcia et al., 1999). The time of
N fertilization in the non-irrigated orchard is DecemberFebruary, in the form
of ammonia or urea. In irrigated orchards N should be supplied in three doses,
i.e. DecemberFebruary as ammonia and the other two doses later in
NO3 form. The amount of precipitation is the determining factor for the
quantity of N applied: if the annual precipitation is < 400 mm we supply
100 g N/tree/100 mm rainfall, while if precipitation is > 700 mm we supply
184
Chapter 18
150 g N/tree/100 mm rainfall. In the latter case the total amount of N applied
would be 1.5 kg N/tree or 15 units N/1000 m2.
Nitrogen should not be applied every year and an optimum level of N
fertilization is required (Marn and Fernndez-Escobar, 1997). In sandy soils N
tends to leach out, causing groundwater and runoff pollution. Therefore, lighter
and more frequent applications are preferable. Conventional N fertilizers such
as urea, ammonium nitrate, ammonium phosphate, ammonium sulphate,
potassium nitrate or calcium nitrate are all suitable. Conventional N fertilizers
have traditionally been used because they are cheap, less bulky and easy to
apply. When combining N fertilization with organic materials the total cost is
considerable. Furthermore, most of the organic materials release their N within
the rst year or two. However, organic materials have the benet of being slowly
released and are less likely to leach into ground or surface waters.
Forms of soil nitrogen
The N found in the soil can generally be classied as either inorganic or organic
(Tisdale et al., 1993). By far the greater total amount of N occurs as a part of
the soil organic matter complex. The inorganic forms of soil N include NH4+,
NO2, N2O, NO and elemental N, which is inert. From the standpoint of soil
fertility the NH4+, NO2 and NO3 forms are of greatest importance.
Nitrogen transformation in soils
Olives absorb most of their N in the NH4+ and NO3 forms. The quantity of these
two ions presented to the roots depends largely on both the amounts supplied
as commercial N fertilizers and released from reserves of the organically bound
soil N. The amount released from the organic reserves or fertilizers depends
on the balance that exists between the processes of N mineralization,
immobilization and soil losses. The mineralization of organic N compounds
takes place essentially in three reactions: aminization, ammonication and
nitrication.
Aminization: protein RNH2 + CO2 + energy + other products
Ammonication: RNH2 + HOH NH3 + R-OH + energy
Nitrication: 2NH4 + 3O2 2NO2 +2H2 + 4H+ (Nitrosomonas)
2NO2 +O2 2NO3 (Nitrobacter)
The ammonia released is subjected to conversion to nitrite and nitrates, for
direct absorption by olive trees; it may be utilized by heterotrophic organisms and
it can be xed in the lattice of expanding-type clay minerals. The NH4+ permits its
absorption and retention by soil colloidal materials in the negative charge of clays
and is not subject to leaching by water, as is the NH3 form. The more
unfavourable the conditions for nitrication and the greater the exchange
capacity of minerals, the longer is the retention time. After nitrication ammonia
is subject to leaching. Of the three forms of N, NO3 and urea are completely
Mineral Nutrition
185
mobile forms in soils and move largely with the soil water. Also, during dry
weather capillary and upward movement of water containing nitrates is possible,
leading to NO3 accumulation in the upper soil zone. Furthermore, it is possible
for NH4+ to be xed by the 1:2 clay minerals having an expanding lattice. The
xed ammonia can be replaced by cations that expand the lattice (Ca2+, Mg2+,
Na+, H+). Nitrogen losses in irrigated olive orchards may occur under the
following conditions:
Denitrication, which is a biochemical reduction of nitrates under

anaerobic conditions; the N losses occur in the form of N2 or N2O.
After surface application of NH4+ or urea in soil with alkaline pH, N is lost
in the form of NH3.
Nutrient stress and growth

When mineral nutrient levels are insufcient for vegetative and reproductive
growth in olive trees, a series of events commence. Hence, N deciency reduces
vegetative growth and N excess promotes vegetative growth, susceptibility to
various pathogens and disturbs the nutrient balance with other elements
especially P. Leaf concentrations of N, P and K are high before maximum yield.
The high N:P ratio reduces lateral shoot growth. Boron concentrations < 15
ppm lead to leaf chlorosis, necrosis, leaf drop, absence of owering and monkeyfaced fruits. Low and high Mn concentrations signicantly affect the chemical
composition of leaves in cultivars tested, i.e. FS-17, Manaki, Kalamon,
Koroneiki and Picual. A genotypic difference was recorded between cultivars,
with Picual accumulating the minimum amount in comparison with other
cultivars. Iron, Zn and B concentrations were not affected; furthermore, Ca was
not affected by Mn levels, although the cvs Manaki, Kalamon and Koroneiki
accumulated the minimum amount of Ca.
Nutrient deciency/excess and photosynthesis
Plants require various nutrient elements in order to effect efcient
photosynthesis, and their responses depend on a particular nutrient. The effect
of a nutrient depends on the photosynthetic step at which it operates.
Nitrogen is a macroelement, and photosynthesis requires signicant
amounts of N. Nitrogen is required in photosynthesis, since it is necessary for
50% of the proteins in thylakoid membranes and in Rubisco, which represents
50% of the total soluble proteins in leaves. Nitrogen deciency reduces
protein and chlorophyll content per unit leaf area, resulting in decreased
photosynthesis.
Phosphorus also affects photosynthesis. Phosphate deciency decreases
the number of leaves, leaf size and the concentrations of proteins and pigments.
However, the photosynthetic component is more or less constant. The ATP
content of tissues is reduced and this leads to a reduced RuBP biosynthesis.
Consequently, the activity of RuBisCO decreases. Manganese concentration
186
Chapter 18
affects the rate (Pn) of photosynthesis. However, a signicant genotypic

difference is obvious. In Koroneiki 640 mg/l Mn increased the Pn. In the other
four cultivars mentioned above, high Mn increased both transpiration rate and
stomatal conductance (Chatzistathis et al., 2006).
Nitrogen excess and quality of olive oil and table olives
Fertilization and irrigation practices can modify the quality of both table olives
and olive oil. Excess N fertilization in olive fruit results in a signicant decrease
in the content of polyphenols, which are the main antioxidants. Furthermore,
a decrease in polyphenols induces a signicant decrease in the oxidative
stability of the oil and accentuates its bitter taste. In contrast, tocopherol
content increases with increasing N concentration in fruit. An excess of N
fertilization does not affect pigment content particularly carotenoid and
chlorophyll pigments nor fatty acid composition.
Excess N fertilization can negatively affect olive production and delay fruit
ripening. A high degree of fertilization reduced polyphenol content and
decreased levels of K225, oxidative stability, the ratio of mono- to poly-fatty acids
and reducing sugars. The reducing sugar content, expressed on a dry weight
basis, was about 17% lower in the two higher fertilizer treatments (i.e. 400 and
600 g N/tree) in comparison with control. Texture, which is an important
organoleptic characteristic in table olives, showed a linear decrease when the
amount of fertilizer was increased. Furthermore, a negative correlation was
found between texture and N content. The loss of texture by increasing the
amount of fertilizer could be due to partial solubilization of cell wall
polysaccharides, through increased water content. Polyphenol content
(mg/kg) was reduced by 33% in the high N treatment, in comparison with
control. The oxidative stability was also reduced, by 34%.
Olive culture and nitrate assimilation
Nitrate is a major form of N available to plants in many environments. Before
NO3 is taken up by the plant it should be reduced to NO2 and, subsequently, to
NH4+. The enzyme catalyzing the reduction of NO3 to NO2 is nitrate reductase
(NR), while the reduction of NO2 to NH4+ is catalysed by the enzyme nitrite
reductase. For NO3 assimilation into organic N two electrons are required, and
six electrons for NO2 to NH4+ reduction. Furthermore, ATP is required for the
assimilation of NH4+ into amino acids and proteins.
Species that reduce NO3 predominantly in their shoot may have the
advantage of being able to use excess reductant released from photosynthesis
(Andrews, 1986; Touraine et al., 1994). In contrast, species reducing NO3
mainly in their roots must obtain reductant from glycolysis and the pentose
phosphate pathway (Oaks and Hirel, 1986). Therefore, the carbon budget is
affected by the plants site of NO3 reduction. Furthermore, ammonium is
incorporated into amino acids by the glutamine synthetase, glutamine-2oxoglutarate transaminase (GS-GOGAT) enzyme system (Therios, 2005a).
Mineral Nutrition
187
Under stress conditions nitrite is also reduced to nitric oxide by NR. Nitric oxide
constitutes a signicant component.
A minor amount of nitrite reduction may also occur during darkness, at
the expense of reducing equivalents generated by the oxidative pentose
phosphate pathway and starch breakdown. The NADH required for NO3
reduction in the cytosol could be provided by photosynthetic electron transport
via malateoxaloacetate or from mitochondrial substrate oxidation.
Olive culture and the nitrate accumulation problem
Nitrate is essential to life, but a hazard when applied in the wrong place and at
the wrong time. Nitrate is present in most natural waters in rain, rivers, lakes,
sea and in the water stored in porous rocks. Nitrate concentrations in natural
water are steadily rising with the increasing use of nitrogenous fertilizer by
olive growers. Furthermore, the European Union (EU) has established a limit of
50 mg/l for NO3 in drinking water, or 11.3 mg/l N.
Due to the common agriculture policy (CAP) of the EU, which ensured a
fair price for agricultural products and the use of extra N, this has resulted
in many cases in excess production; high rates of N fertilization have resulted
in NO3 pollution. The solution is not simple, since a reduction in use of
nitrogenous fertilizer results in lower agricultural income. Furthermore,
lowered productivity raises food prices in the supermarket.
Nitrate itself is not toxic but becomes problematic after its conversion into
nitrite, which is responsible for blue baby syndrome, or methaemoglobinaemia,
and stomach cancer. The blue baby syndrome is common in children under 1
year old who consume a certain level of nitrates. Microorganisms convert NO3
to NO2 and NO2 reacts with haemoglobin, which transports oxygen through
the body, and haemoglobin is transformed into methaemoglobin. The Fe in
haemoglobin is in the ferrous form, but in the ferric form in methaemoglobin.
Therefore, the O2-carrying capacity of haemoglobin is reduced. Stomach cancer
has also been linked to the NO3 concentration in drinking water. Nitrite
produced from NO3 reacts with secondary amines, producing N-nitroso
compounds that can modify DNA. The World Health Organization (WHO) has
set two limits, one of 50 mg/l the recommended one and a maximum of 100
mg/l (22.6 mg/l of N).
When more N is applied, the increase in yield per kg unit of N reduces
(diminishing returns). When the soil contains adequate N the curve attains
the form of a straight line, which declines continuously as N increases.
Therefore, additional N reduces yield. The third form of N curve is that in which
additional N increases the yield steadily, up to a point above which it sharply
declines.
Nitrate leaching from olive orchards
Nitrate-containing fertilizers, when applied to the soil surface and after their
entrance into the soil by irrigation or rainfall undergo a process known as
188
Chapter 18
dissociation, i.e. separation into anion and cation. The soil solution should
contain equal numbers of positive and negative ions, due to the principle of
electroneutrality.
The ammonium ion (NH4+) is the other ion containing N, and its relation
to ammonia (NH3) is given by the equation
NH OH NH + + OH
NH3 + H2O
4
4
Therefore, when ammonia (NH3) is dissolved in water this gives an
alkaline solution. Reactions proceed in both directions towards the left/right
or clockwise/anti-clockwise, meaning that in alkaline soils NH4+ can be
converted to NH3 gas and therefore lost to the air. The clays in soil readily
accumulate electrical charges, the overall charge being positive at very acidic
pH and negative at pH in the range 5.58.0. This indicates that positively
charged ions are attracted to the surface of the clay, but negatively charged ions
such as NO3 are repelled and are more vulnerable to downward leaching.
Ammonium is strongly attracted to the negative charges of clay particles
and does not leach out in soils with adequate clay content. Use of nitrication
inhibitors reduces the speed of NH4+ transformation to NO3 by soil bacteria and
reduces NO3 leaching. Such inhibitors are based on pyridine and are
circulated under the commercial names N-Serve or Dicyandiamide (DCD).
Furthermore, such transformation in the soil is inhibited by the alkaline soil
reaction. Leaching of NO3 requires liquid water (not gas or ice) and a soil
saturated or partly saturated. Therefore, we can describe two ows of water in
the soil saturated and unsaturated. The main force of NO3 leaching is gravity,
or downward movement, of water and NO3.
Water containing NO3 can leach downwards by pushing down on the
water containing NO3 like a piston on an equivalency basis. For example,
20 mm of precipitation or irrigation displaces an equivalent soil water volume.
If we hypothesize that soil porosity is lled with water and air 50/50, then the
depth of NO3-containing leaching water is 20/0.5 = 40 cm. Nitrate loss by
leaching is greater in soils with low water-holding capacity and in soils with
rapid water movement, i.e. in soils with high hydraulic conductivity.
Therefore, soil texture and its volumetric H2O content are determining
factors of NO3 leaching. Other factors affecting NO3 leaching include: (i) the
microorganism population; (ii) the organic matter content of those olive groves
with a high degree of organic culture; and (iii) a higher root density of olive
groves, which increases NO3 absorption and reduces the risk of NO3 leaching.
The simplest model is the piston ow, the analogy of which is a piston
pushing out or displacing uid from a cylinder. This model is expressed by the
equation
Zp = /
where Zp = the depth to which the front of the displacing solution
penetrates; = the quantity of water or solution necessary for displacement;
Mineral Nutrition
189
= the volumetric moisture content, i.e. the percentage of soil volume that
can hold water.
This equation means that, the more water the soil can hold the smaller the
downward movement that is caused by a given amount of applied water. This
model works best in sandy soils, and it deals only with a single application of
NO3. The main source of NO3 leakage is organic material in the cultivation
system called organic farming.
Organic olive orchards
Organic farmers point out that the large inputs of chemicals to the soilplant
system upsets the natural ecosystem. Organic farmers use manures to fertilize
olive orchards or they grow a legume crop, such as beans, in order to bring N
into the soil. Plants must take up mineral N whether they are grown
conventionally or organically. The high availability of N from chemical
fertilizers encourages rapid growth and large yields. Organic farmers produce
fewer yields but of higher quality and, for these products, people are prepared
to pay more. To obtain yields similar to those of conventional farmers,
organic farmers should supply with organic matter a similar amount of
mineral N.
Organic olive farming has increased in Greece and represents 2.5% of the
total area of olive cultivation and about 50% of all organic farms. The use of
composts in the organic groves supplies the olive tree with all the essential
nutrients for achievement of adequate nutritional status, i.e. normal
productivity. The use of compost increases the soil organic matter content. The
soil pH values of organic orchards are higher than those of the conventional,
the lower pH values of the latter being the result of the application of mineral
fertilizers and more frequent cultivation. Concerning the N level of leaves this is
marginal in organically grown olives, while P and K concentrations in the
leaves are greater in organic farming.
With regard to micronutrients, Fe, Mn, Zn and Cu can be bound to chelates
in organic matter, and their concentration in leaves is lower in comparison
with non-organic olive groves. Boron levels are higher in organic than in
conventional orchards. Furthermore, irrigated conventional and organic
farming orchards have higher concentrations of leaf P than the non-irrigated.
Nitrate leaching losses as affected by traditional and slow-release fertilizers
Olive trees require N in greater amounts than other mineral nutrients, but less
than 20% of the N applied to the olive orchard is taken up by the fruit trees. The
use of traditional fertilizers increases N leaching, reduces N utilization
efciency (NUE) and also increases the pollution of ground water. The use of
slow-release fertilizers achieves a step-wise supply of nutrients for a long
period. This increases NUE and reduces NO3 leaching. However, although the
use of slow-release fertilizers is advantageous, their use is very limited due to
the economic cost in comparison with the low income from olives, especially of
190
Chapter 18
non-irrigated olive groves.

Some of the best-known slow-release N fertilizers are circulated under the
names Floranid, Multicote, Basammon, etc. (Fernndez-Escobar et al., 2004a).
Floranid signicantly increases N concentration in leaves in comparison with
other fertilizers such as ammonium sulphate, ammonium nitrate, calcium
nitrate and Basammon. Most of the total N (> 60%) of olive plants is found in
the leaves. Nitrate is the most common form of N leaching. However, NO2N
leaching can be observed when the orchards are fertilized with urea or
ammonium sulphate. Another form of N leaching is that of NH4 when the
fertilizer Basammon is used. With respect to traditional fertilizers, urea and
ammonium sulphate result in lower N losses.
KINETICS OF NITRATE ABSORPTION BY OLIVE PLANTS

Nitrate is the nal N form in soils well aerated and drained, as occurs with olive
orchards. The factors affecting NO3 absorption by olive plants include: (i)
induction period; (ii) NO3 and Ca concentrations; (iii) pH of the nutrient
solution; (iv) temperature in the root environment; (v) N deciency; (vi) NH4
concentration; and (vii) light intensity (Therios, 1981). These measurements
were conducted in a hydroponic system (see Fig. 18.2).
Induction period
The rate of NO3 absorption is signicantly less in the rst hours than later on.
This reduced rate of absorption (lag phase) is the required time for induction of
the enzyme NR. Nitrate absorption has two stages, the rst due to active
absorption and the second due to the induction of NR (reduction); the ratio
between absorption and reduction is 3:1.
The effect of substrate nitrate concentration on absorption rate

When the external NO3 concentration in solution changes from 0.06 to 0.60
mM, the rate of NO3 absorption is a function of the external concentration
(Therios, 1981). The maximum rate of absorption (Vmax) is 0.7 mol/g/h f.w.
of roots and the Km value is 0.23 mM. When we calculate 1/V versus 1/S a
straight line is produced, indicating that Chondrolia Chalkidikis follows
MichaelisMenten kinetics. Increase in the external concentration to 2 mM
NO3 increases the rate of NO3 absorption above the Vmax value. These data
indicate the existence in olives of two mechanisms at work: (i) at lower
concentrations than 0.6 mM; and (ii) at greater concentrations than 0.6 mM.
The Vmax of olives is 2550% of the NO3 absorption rate reported in Prunus
Rate of NO3 absorption mol/g/h
Mineral Nutrition
1.0
191
1V
8
(a)
mol/g/h
0.6
0.4
0.2
(b)
Vmax = 0.7 i mol/g/h

Km = 0.23 mM
0.8
6
4
2
1
Vmax
1
Km
0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00
8
12
18
1/NO3 (mM)
NO3 (mM)
20
2.0
(d)
Rate of NO3 absorption mol/g/h
NO3 absorption mol/g/4h
(c)
1.6
1.2
0.8
0.4
6
pH
0.6
0.5
0.4
0.3
0.2
0.1
10
15
20
25
30
35
Temperature (C)
0 0.25 0.50
2
CaSO42H2O (mM)
(f)
4
Rate of NO3 absorption mol/g/9h
NO3 absorption mol/g/5h
(e)
0.50
2
NH4+ (mM)
Fig. 18.2. Rate of NO3 absorption by olive plants. (a) Effect of NO3 concentration;
(b) Lineweaver-Burk plot; (c) pH effect; (d) effect of temperature; (e) effect of calcium
concentration; and (f) effect of NH4+ concentration (from Therios, 2006).
192
Chapter 18
domestica (Therios et al., 1979) and 10% of that reported for barley. The low
Vmax value and the high Km value (0.23 mM) indicate that olive trees are not
efcient in NO3 absorption. Therefore, in olives NO3 fertilization would be
expected to lead to greater N losses due to leaching, in comparison with
Marianna 2624, with a Km value of 0.06 mM. This point is not very important
in non-irrigated olive orchards, while in irrigated ones more NO3 losses by
leaching would be expected. Hence the fertilization programmes for olives
should be carefully planned: N should be applied in many and small doses in
order to avoid leaching losses. A good solution is to apply N in the form of NH4
and to use inhibitors of nitrication, such as N-Serve (nitrapyrin) or other
forms of slow-release N fertilizers.
pH and absorption of nitrate

Nitrate absorption is greater in acid nutrient solutions and decreases by
increasing the pH values. No optimum pH value for N absorption has been
recorded for olives. At pH 8.0 NO3 absorption is 30% of that absorbed at pH 6.0.
Nitrate absorption is affected more by the OH than by H+ ions. With regard to
the NH4 form, its absorption is less at acidic pH (6.0), due to H+ competition,
and greater at alkaline pH. The root has the capacity to modify the pH in its
microenvironment. Hence, when roots absorb more cations than anions the
pH of the surrounding medium is reduced due to H+ extrusion.
The effect of temperature on nitrate absorption

The rate of NO3 absorption viz--viz soil solution temperature shows that the
relationship tends to be linear at temperatures of 1530C. Temperatures
<15C signicantly reduce the rate of absorption, which is almost zero at 5C.
The Q10 quotient for temperatures of 2030C is about 2.0. This indicates that
NO3 absorption in olives is a metabolic process. These data indicate that NO3
fertilization of the olive is ineffective during low winter temperatures.
Furthermore, during this period the danger of NO3 leaching is increased.
The effect of calcium concentration on nitrate absorption

An increase in Ca concentration results in an increased rate of NO3 absorption.
The relation between V (rate of absorption) and Ca concentration is a hyperbola.
The optimum concentration of Ca is 0.5 mM; greater Ca concentrations do not
affect the rate of NO3 absorption. When Ca is excluded from the nutrient solution
the rate of NO3 absorption is 50% less than the maximum value. Calcium exerts
a signicant effect on plasmalemma selectivity and semipermeability.
Mineral Nutrition
193
The effect of various anions and cations on nitrate absorption

The cations K+, Ca2+, Mg2+ and NH4+ affect NO3 absorption. Greater NO3
absorption is found when K is used as the carrier of NO3. Nitrate absorption is
90% when Mg and Ca are the NO3 carriers. The minimum NO3 absorption occurs
when NH4+ is the accompanying cation. The rates of NO3 absorption are: 77%
from NaNO3, 90% from Ca(NO3)24H2O or Mg(NO3)2 and 46% from NH4NO3.
The anions Cl and SO42 do not reduce NO3 absorption (see Table 18.5).
The effect of nitrogen deciency on nitrate absorption

Nitrate exclusion or its low concentration in soil solution for a number of days
increases the rate of NO3 absorption. The maximum rate of NO3 absorption
was recorded on the tenth day of deciency.
The effect of ammonium on nitrate absorption

Ammonium decreases the NO3 absorption rate. By increasing NH4+ concentration to 0.5 mM in the nutrient solution the rate of NO3 absorption
decreases by 46% (Therios, 1981). At greater NH4+ concentrations the NO3
absorption rate is 25% of the control. The decrease in NO3 absorption is related to
either the reduced rate of utilization of NO3 by plants or the effect of NH4 on the
NO3 reduction system. The decrease of NO3 absorption in the presence of NH4
may be ascribed to the acidity created due to faster absorption (more cations) of
NH4 in comparison with NO3. This results in a reduction of plasmalemmal
permeability. Furthermore, NH4 may displace Ca from plasmalemma, or the NO3
efux in the presence of NH4 may be reduced. Moreover, NH4 may inhibit noncyclic phosphorylation and therefore NO3 absorption.
Table 18.5. The effects of various cations and anions on NO3 absorption by olive
plants (from Therios, 1981).
Ion
K+
Na+
Ca2+
Mg2+
NH4+
SO42
Cl
NO3 absorption
(mol/g/10 h)
Relative rate of NO3 absorption

(K = 100%)
5.2
4.0
4.7
4.8
2.4
5.3
5.1
100
77
90
92
46
102
98
194
Chapter 18
The effect of light intensity on nitrate absorption

Light affects NO3 absorption since it controls photosynthesis and the
production of ATP (energy) required for active absorption. Light is also
important for the induction of the enzyme nitrate reductase (NR).
Ammonium absorption
The classical enzyme kinetics is followed in NH4+ absorption, which resembles
the absorption of other monovalent ions and especially that of potassium. This
is the reason that a high NH4+ concentration competes with K+, although the
afnity for the common point of attachment is only 10% that of K+. Plants
growing under acidic soil pH are obliged to absorb NH4+ rather than NO3-N.
Furthermore, the absorption of NH4+-N by olive plants is greater than the
NO3 or urea N forms. Two systems of NO3 transport have been proposed: the
rst of these has a high afnity for NO3; it works at 5100 M NO3
concentration and consists of the NRT1 genes. The Vmax ranges between 3 and
8 mol/g/h. The second, the low-afnity system, uses the NRT2 genes and
works at concentrations > 250 M NO3. Both systems are activated by the
presence of NO3 in the external solution. The lack of NO3 induces NO3
absorption and increases the rate of its absorption.
THE EFFECT OF NITROGEN FORM ON THE GROWTH AND

MINERAL COMPOSITION OF OLIVE PLANTS
Experiments with several plant species comparing the three N-forms (NO3-N,
NH4-N and urea-N) have shown that the form of N supplied exerts a
pronounced effect on both growth (Tattini et al., 1990; Garcia et al., 1999;
Therios, 2006) and chemical composition, as also noted in olives (Therios and
Sakellariadis, 1988; Tsambardoukas, 2006).
Recently, the rapid expansion of olive culture has demonstrated the need
for integrated studies concerning their growth and nutrient requirements. One
such study involved the growth response of olives to N forms. The mean fresh
weight per plant was increased signicantly by increasing the N level from 1 to
8 mM. This increase in N concentration induced signicant increased growth
for the NH4-N and urea-N treatments, while with NO3-N growth was reduced.
Root morphology was affected to a lesser degree by the NH4-N forms,
producing at 16 mM a root system with shorter and thickened feeder roots in
comparison with those treated with NO3-N and urea. The growth reduction at
16 mM NO3-N was probably due to limited P absorption by the olive plants.
The presence of Ca in all the nutrient solutions in our study minimized the toxic
Mineral Nutrition
195
effect of NH4-N. Based on the fact that the greatest growth coincides with the
greatest N concentration in leaves and also that most olive orchards are well
supplied with Ca, we may conclude that the NH4-N form is the most preferable
source of N for olives. Other processes should also be considered, such as a pH
decrease with increased (NH4)2SO4 supply or the possibility of NH3 loss at
higher pH values and dry soil conditions. Addition of Ca to the NH4-N form
may increase ammonium absorption and accumulation. In the presence of an
adequate supply of carbohydrates, NH4-N assimilation should proceed more
rapidly than NO3-N assimilation. It is therefore not surprising that greater
concentrations of N were found in the NH4-N-fed plants.
The N form did not signicantly affect K and Fe concentrations. However, Ca
concentration was affected by the N form. In our experiments we tested the effect
of NO3, NH4, urea or NO3 + NH4 on the mineral composition of leaves. Again, the
greatest accumulation of N was recorded with 16 mM NH4 or NH4 + NO3 (see
Table 18.6). The same was recorded for K. However, the greatest accumulation of
P was recorded with urea 116 mM or 1 mM NH4 + NO3. Also, the greatest Mg
concentration was recorded with 1 and 8 mM NH4 + NO3. The greatest Mn
concentration was recorded with 16 mM NH4 in the top and basal leaves. Zinc
concentration was greatest with 1 mM urea or 1 mM NH4 + NO3. Urea and NH4
+ NO3 resulted in the greatest concentration of Fe. Our data also indicated an
effect of the N form on the photosynthetic rate (Tsambardoukas, 2006).
Nitrogen assimilation
Ammonium absorbed by olives is a reduced form of N and could be used by the
plants to produce amino acids. Nitrate absorbed should previously be reduced
Table 18.6. Effect of N form (NH4+, urea and NH4+ + NO3) on mineral
composition of olive leaves (cv. Kalamon) at three concentrations (from
Tsambardoukas, 2006).
NH4+
Mineral
N*
P*
K*
Mg*
Fe**
Mn**
Zn**
1 mM
8 mM
NH4+-NO3
Urea
16 mM
1 mM
8 mM
16 mM
1mM
1.66b
1.98cd
2.21e
1.61b
1.96cd
ab
bc
de
e
0.21
0.23
0.32
0.37
0.36de
abc
bcd
e
de
1.10
1.21
1.48
1.31
1.25cd
0.57de
0.50bcd 0.40a
0.46abc 0.43ab
40ab
62bc
67c
69c
68c
a
c
e
abc
10
27
56
15
17abc
a
c
c
e
8
17
17
24
17c
2.07de
0.39e
1.20bcd
0.54cde
65c
42d
18d
1.66b
0.39e
1.06bc
0.48abcd
56bc
15ab
24de
8 mM
16 mM
1.97cd 2.19e
0.29cd 0.14a
1.26cd 1.45e
0.60e 0.46abc
62c
65c
bc
22
49de
bc
14
16c
*%; **ppm. Superscript letters in the same column denote statistically signicant differences
for P 0.05 (Duncans multiple range test).
196
Chapter 18
to NH4 in order to be assimilated (Pilbeam and Kirkby, 1992). This NO3

reduction to NH4 is completed in two stages. The rst stage is catalysed by the
enzyme nitrate reductase (NiR) located in the cytoplasm, which converts NO3
ions to NO2 (Campbell, 1988). The second stage is catalysed by NiR, which
converts NO2 to NH3 and is located in the chloroplast.
The NO3 reduction is expressed by the equation:
NiR
NiR
NO3 + 2e (NADH or NADPH+) NO2 +6e (fd) + 7H+ NH3 + 2H2O
Subsequently, NH4+ is incorporated into organic molecules with the help
of four enzymes and reactions (Nathawat et al., 2005):
Glutamic dehydrogenase (GDH).

GS.
GOGAT.
Asparagine synthetase.
The following equations are then used:
-ketoglutaric acid + NH4+ + NAD(P)H L-glutamic + NAD(P)
Glutamic acid + NH4+ + ATP Glutamine + ADP + H3PO4
Glutamine + -ketoglutaric acid 2Glutamic acid
Aspartic acid + NH4+ + ATP Asparagine + ADP + P
Potassium
Roles of potassium
Potassium is important for its involvement in the following processes (Tisdale
et al., 1993).
Carbohydrate metabolism.
Metabolism of N and protein synthesis.
Enzyme activity.
Regulation of the opening and closing of stomata.
Improvement in fruit quality and disease tolerance.
Alteration of photosynthesis and respiration and activation of the enzymes
peptase, catalase, pyruvic kinase, etc.
Potassium plays an important role in olive nutrition. Potassium deciency

represents 62% of nutrient deciencies in olives. The optimum K concentration
of leaves is 0.70.9% (leaves collected in winter from the middle of the last ush
of growth, 58 months old).
Mineral Nutrition
197
Potassium in the soil

Potassium is absorbed by olive plants in greater amounts than any other
element, excepting N. Potassium is present in relatively large quantities in most
soils; the K content of the earths crust is 2.4%. Of the total amount of K in
most soils, only a fraction can be immediately utilized by plants. Soil K exists in
three forms: (i) relatively unavailable; (ii) slowly available; and (iii) readily
available. These three forms are considered to be in equilibrium (Tisdale et al.,
1993).
Unavailable K occurs as a part of the crystal structure of unweathered
primary and secondary micaceous and feldspathic minerals. Readily available
K occurs in the soil solution and on the exchange complex and is readily
absorbed by plants. Slowly available K becomes available to plants slowly
and over a longer period of time: slowly available K+ exchangeable K+
water-soluble K+.
Potassium can be xed in soils high in 2:1 minerals, especially those such
as illite. Ammonia is almost of the same ionic radius as the K+ ions and is
subject to xation by 2:1 clays. Because NH4+ can be xed by clays in a manner
similar to the xation of K, its presence will alter both the xation of added K
and the release of xed K. An interaction of NH4NO3 and K nutrition has been
reported (Hagin et al., 1990).
Potassium needs and fertilization
When the K concentration of leaves drops to 0.3% and symptoms of K
deciency appear, the application of 620 kg K2SO4/tree is recommended; a
greater amount for heavy soils. For a concentration of 0.30.5%, 410 kg
K2SO4/tree is suggested; for a concentration of 0.7% K is applied at twice the
rate of N; and for > 0.9% no K fertilizer is added. Potassium fertilizers are
applied during autumn or winter and are incorporated into the soils. Some
suggest the addition of 0.81.0 kg K/tree for non-irrigated olive orchards and
1.01.5 kg K for irrigated. Fertilization with K is necessary in shallow soils
with high CaCO3 and also in acid soils.
Phosphorus
Roles of phosphorus
Phosphorus is a component of high-energy substances such as ATP, ADP and
AMP; it is also important for nucleic acids and phospholipids (Olsen and
Sommers, 1982). Phosphorus affects root growth and maturation of plant
tissues and participates in the metabolism of carbohydrates, lipids and proteins.
Due to its extensive root system the olive tree absorbs adequate quantities of P.
Therefore, some investigators have proposed that P fertilization is not
necessary. The ideal P concentration of leaves is 0.090.11%. However, P
198
Chapter 18
fertilization is applied only when there are certain indications of a true

response to P. When P concentration is raised above the critical value, K
concentration is reduced. Phosphate fertilization could be tried under the
following circumstances: (i) in shallow and poor soils; (ii) in orchards fertilized
for long periods only with N fertilizers; (iii) in soils high in CaCO3; and (iv) in
soils of low pH. When P fertilization is necessary the ratio of P:N in the fertilizer
should be 1:3.
Soil and phosphorus fertilizers
The total P content varies from soil to soil (Tisdale et al., 1993). The available P
level in fertilized and cultivated soils is high because little of this element is lost
in percolating water from most soils and crops. Soil P can be classed generally
as either organic and inorganic. The organic fraction is found in humus, while
the inorganic occurs in numerous combinations with iron, aluminum,
calcium and other elements. These compounds are usually only very slightly
soluble in water. Phosphates also react with clays to form insoluble
clayphosphate complexes. Phosphorus is absorbed by plants largely in the
form of the ions H2PO4 and HPO42 that are present in the soil solution. The
surface actively absorbing P on plant roots is near the tips.
Organic soil phosphorus
Organic P occurs in the form of phospholipids, nucleic acids and inositol
phosphates. The mineralization of organic P has been studied in relation to the
ratio C:N:P in the soil. A C:N:P ratio of 100:10:1 for organic matter has been
suggested.
Inorganic soil phosphorus
Plants absorb P largely in the forms H2PO4 and HPO42. The concentration
of these ions in the soil solution at any time is low, generally never more
than a few parts per million. The crop removal of P is usually in the range
0.42.0 kg/1000 m2 of orchard. Phosphorus in the soil solution must be
continuously replaced or the crop would not have sufcient P to grow to
maturity. The concentration of the various phosphate ions in the solution is
intimately related to the pH of the medium. The H2PO4 ion is favoured in more
acid media, whereas the HPO42 is favoured at pH above 7.0. Phosphate
retention occurs in acid soils after reaction with Fe and aluminum. In alkaline
soils, which are very common in olive culture, precipitation of calcium
phosphate is very common.
Magnesium
Magnesium plays an important role in chlorophyll synthesis and also as an
enzyme activator. Healthy olive leaves contain 0.10.3% Mg, and below 0.1%
Mineral Nutrition
199
Mg deciency occurs. The important factor is not the concentration of Mg but

the Ca:Mg ratio (Demetriades et al., 1960b). Some cases of abnormal olive
nutrition have been reported, even with 0.64% Mg, due to the antagonism of
Mg to Ca (Therios and Sakellariadis, 1982). Magnesium deciency problems
are solved by application of 2.0 kg/tree of MgSO47H2O or by spraying with a
2% solution of MgSO47H2O.
Calcium
Calcium is the element that participates in the integrity and semipermeability
of the plasmalemma. Calcium also regulates soil acidity and improves soil
texture. The Ca concentration of leaves is 1.04.5%. Deciency is recorded
when Ca is < 0.5%. In order to redress Ca deciency we add 2001000
kg/1000 m2 of CaCO3 dust during the autumn and incorporate this into the
soil.
Micronutrients
Iron
Iron is one of the most common of the metallic elements in the earths crust. Its
content ranges from a low of 200 ppm to more than 10%. It occurs in soils as
oxides, hydroxides and phosphates, as well as in the lattice structure of primary
silicate and clay minerals. Total Fe content is of no value in diagnosing Fe
deciencies.
Iron deciency is believed to be caused by: (i) an imbalance of metallic ions,
such as Cu and Mn; (ii) an excessive amount of P in soils; (iii) high pH; (iv) high
soil moisture; (v) cool temperatures; and (vi) high levels of HCO3 in the rooting
medium (Benitez et al., 2002). Iron deciency chlorosis in olive could be
corrected by using a low-pressure trunk injection method (Fernndez-Escobar
et al., 1993). High HCO3 levels in the soil increase the solubility of P and result
in a large uptake of this element, which interferes with Fe metabolism in the
plant. Excessive phosphate fertilization will also induce Fe chlorosis.
Manganese
Soil Mn originates from the decomposition of ferromagnesian rocks.
The quantities vary from a trace to several thousand kg/ha. Manganese
deciency has been reported and may be induced by an imbalance of other
elements such as Ca, Mg and ferrous Fe. Manganese in the soil is generally
considered to exist in three valency states: (i) divalent Mn2+ soil solution;
(ii) trivalent Mn existing in the form of the oxide, Mn2O3; and (iii) tetravalent
200
Chapter 18
Mn (Mn4+), which exists as the very inert oxide MnO2. The three forms exist in
equilibrium.
Mn MnO2
(Mn2+)
(Mn4+)
Mn2O3
(Mn3+)
Manganese deciency is very common at high pH values. Low and high
Mn concentrations (10, 40, 160, 640 M) signicantly affect the chemical
composition of the leaves in the cvs FS-17, Manaki, Kalamon, Koroneiki
and Picual (Chatzistathis et al., 2006). However, a genotypic variation has
been recorded among the cultivars, and the cv. Picual accumulated the least
amount in comparison with the other tested cvs. Iron, Zn, B and Ca were not
affected by Mn levels. Manganese concentration affected the Pn rate (Pn), and
a signicant genotypic variation was recorded, with Koroneiki increasing its
Pn with 640 M Mn. The leaf uorescence (Fv/Fm) was decreased by
increasing the Mn concentration (see Table 18.7).
Boron
Boron occurs in most soils in extremely small quantities, ranging from 20 to
200 ppm, and B deciency is common in olive trees (Demetriades et al.,
1960a). During owering and fruit development mobilization of B has been
recorded in olive trees (Delgado et al., 1994). In most cases B does not normally
occur in toxic quantities in most arable soils, unless it has been added in
excessive amounts with commercial fertilizers. However, there are arid regions
in which soils contain this element in toxic quantities, but these areas are few
in number and of little agricultural value. Native B in most humid-region soils
is in the form of tourmaline.
Most of the available soil B is held by the organic fraction and it is retained
rather tightly. As the organic matter decomposes the B is released; part is taken
up by plants and part is lost by leaching. Some B is held by clay or adsorbed by
the inorganic fraction of soils. The principal sites of such adsorption are
thought to be: (i) Fe and Al-hydroxy compounds present as coatings on or
associated with clay minerals; (ii) Fe or Al oxides; and (iii) clay minerals,
especially of the micaceous type. Coarse-textured, well-drained, sandy soils are
low in B. The fact that clays retain B more effectively than sandy soils does not
necessarily imply that plants will absorb this element from clays in greater
quantities than from sands. On the contrary, plants will take up much larger
quantities of B from sandy soils. The practical implication of these data is of
Mineral Nutrition
201
Table 18.7. Effects of four Mn concentrations on the mineral composition of the

olive cultivars FS-17, Manaki, Kalamon, Koroneiki and Picual (from Th.
Chatzistathis, personal communication).
Mn concentration
(M)
FS-17
0 (control)
10
40
160
640
Manaki
0 (control)
10
40
160
640
Kalamon
0 (control)
10
40
160
640
Koroneiki
0 (control)
10
40
160
640
Picual
0 (control)
10
40
160
640
Mn*
Fe*
Zn*
Ca**
Mg**
K**
P**
B*
24a
24a
46a
147b
591c
84a
67b
68b
59b
67b
14a
16a
19a
16a
21a
2.67b
2.64b
2.70ab
2.68b
2.81a
0.12a
0.11a
0.07b
0.10a
0.12a
2.09a
2.06a
2.06a
2.05a
1.74b
0.16c
0.22bc
0.31a
0.27ab
0.31a
40a
31b
39a
38a
38a
20a
44ab
72ab
125b
734c
85ab
88ab
98a
80ab
63b
12c
16b
15bc
15bc
21a
1.11b
1.18a
1.17a
1.08bc
1.04c
0.11b
0.12ab
0.12ab
0.12ab
0.13a
1.90a
1.91a
1.88a
1.90a
1.88a
0.27ab
0.25ab
0.28ab
0.24b
0.30a
44ab
37b
41ab
43ab
47a
15a
21a
44a
129b
625c
81a
84a
86a
93a
62b
13a
15a
14a
15a
13a
0.92a
0.95a
0.91a
0.84a
0.89a
0.12a
0.11ab
0.12a
0.10b
0.11ab
1.59ab
1.57ab
1.60ab
1.68a
1.48b
0.15a
0.18b
1.15a
0.14a
0.17ab
23a
23a
25ab
24ab
27b
13a
20a
25a
78b
708c
63a
53a
58a
60a
56a
11b
15a
12b
9b
12b
1.40a
1.43a
1.37a
1.32ab
1.19b
0.16bc
0.20a
0.17b
0.16bc
0.14c
2.50a
2.25a
2.17a
2.59a
2.41a
0.18b
0.15b
0.26a
0.23a
0.24a
30ab
35a
35a
31a
25b
8a
15a
24a
40a
189b
87a
69a
78a
80a
74a
18ab
22a
16ab
19ab
15b
1.68ab
1.78a
1.55ab
1.39b
1.56ab
0.19a
0.21a
0.18a
0.20a
0.19a
1.47a
1.35a
1.76b
1.59ab
1.49ab
0.16a
0.15a
0.13a
0.13a
0.16a
46a
42ab
33c
32c
35bc
Superscript letters in the same column denote statistically signicant differences for P 0.05
(Duncans multiple range test). *, microelements; **, macroelements.
course that rates of applied water-soluble B fertilizer should be lower on coarsetextured sandy soils than on ne-textured soils for the same degree of expected
plant uptake.
Boron is one of the essential nutrients necessary for plant growth. In
nature B toxicity is not as common as B deciency. Boron toxicity that limits
plant growth can be observed under certain conditions such as soils with high
B content, overdosing with fertilizers and irrigation with water high in B
202
Chapter 18
(Chatzissavvidis, 2002; Chatzissavvidis et al., 2005). Safe concentrations of B

in irrigation water range from 0.3 mg/l for sensitive plants to 1.02.0 mg/l for
semi-tolerant plants, like olives, and to 2.04.0 mg/l for tolerant plants. In
closely related species, or in cultivars of the same species, genotypes susceptible
to B toxicity generally give higher B concentration in plant tissues than do
tolerant genotypes. Rootstock affects B concentration at the canopy of the olive
tree (Chatzissavvidis and Therios, 2003).
An olive culture area with irrigation water high in B (3.6 m/l) exists in
northern Greece (Chalkidiki). Leaf samples were collected over a period of
24 months and analyzed for N, P, K, Ca, Mg, Fe, Mn and Zn. The results indicated
that olive is a species relatively tolerant to excess soil B, and no visual symptoms
of B toxicity were observed on leaves. The two tested cultivars (Chondrolia
Chalkidikis and Amphissis) followed a similar pattern of uctuations in B
concentration over the summer (see Figs 18.3 and 18.4) (Chatzissavvidis et al.,
2007). The maximum B concentration in leaves was 175 mg/kg dry matter in
Chondrolia Chalkidikis and about 70 mg/kg dry matter in Amphissis. Boron
concentration in owers was higher than that in the leaves. The seasonal
variation in B concentration is also presented in Figs 18.3 and 18.4. The
minimum B concentrations in the previous years leaves of Chondrolia
Chalkidikis were found during owering (see Fig. 18.5) (Chatzissavvidis et al.,
2005). The number of orets per inorescence declined from 13.4 to 10.8 for
Chondrolia Chalkidikis and from 21.5 to 19.7 for Amphissis (Chatzissavidis et
al., 2004). This decrease could be attributed partly to the relatively low leaf N
(1.53%) during owering. Fruit B concentration (see Table 18.8) increased by
only 160% for Chondrolia Chalkidikis, with a maximum value of 122 mg/kg.
The form of nitrogen (NO3, NH4, etc.) under a high B content of the medium
modied the nutrient levels of olive plants (Chatzissavvidis et al., 2007).
Fig. 18.3. Seasonal variation in B concentration in leaves of the current and

previous years (cv. Amphissis) (from Chatzissavvidis et al., 2004).
Mineral Nutrition
203
Fig. 18.4. Seasonal variation in B concentration in leaves of the current and

previous years (cv. Chondrolia Chalkidikis) (from Chatzissavvidis et al., 2004).
B concentration (mg/kg d.m.)
90
0 mg B/l
80
70
Blooming
0.5
5.0
Blooming
60
50
Harvesting
40
30
20
10
0
J
97
F M A M J
A S O N D J F
Month/Year
M A M J
98
Fig. 18.5. Seasonal variation in B concentration in leaves of cv. Chondrolia

Chalkidikis as affected by B level in the nutrient solution (from Chatzissavvidis
et al., 2005).
RESPONSE OF OLIVE TREES TO SALINITY

It is generally accepted that many stresses inducing salinity also induce an
overproduction of reactive oxygen species (ROS) such as hydrogen peroxide,
superoxide radical and hydroxyl radicals, and these compounds are thought to
be responsible for the oxidative damage associated with plant stress.
Olive is moderately salt tolerant, although salinity may be a problem due to
the high salt concentration in the irrigation water used in the Mediterranean
area. Olives can be irrigated with water containing up to 3200 ppm of salt with
204
Chapter 18
Table 18.8. Seasonal variation in concentrations of selected nutrient elements in

olive fruits (cv. Chondrolia Chalkidikis) (from Chatzissavidis et al., 2004).
Nutrient element
B (mg/kg d.m.)
N (% d.m.)
P (% d.m.)
K (% d.m.)
Fe (mg/kg)
Zn (mg/kg)
Days after full bloom
Nutrient concentration
30
60
90
120
30
60
90
120
30
60
90
120
30
60
90
120
30
60
90
120
30
60
90
20
47a
47a
96ab
122b
1.22f
0.73b
0.83cd
0.90de
0.15d
0.11c
0.10bc
0.11c
0.94bc
0.89ab
1.71e
1.63e
56ab
84b
18a
17a
18c
16bc
13b
11b
Superscript letters in the same column denote statistically signicant differences for P 0.05
(Duncans multiple range test).
a SAR of 26 or lower. It is estimated that approximately one-third of the worlds

irrigated lands and half the lands in semi-arid and coastal regions are affected
by salinization; 10 million ha of irrigated lands are abandoned annually
because of excessive salinity. Hence, an effective way must be found to use soil
of high salinity by the cultivation of tolerant cultivars (Tabatabaei, 2006).
Tolerance to salt appears to be cultivar-dependent (Therios and Misopolinos,
1988). For example, 80 mM NaCl may increase or decrease the percentage of
perfect owers in different cultivars. Salinity reduced the growth of all cultivars
tested to varying degrees. A reduction in growth and total leaf area was
recorded by increasing the concentration of NaCl in the nutrient solution
(Vigo, 1999; Vigo et al., 2005). Furthermore, Pn was reduced and stomata
were closed above a threshold salinity. The cultivar Kalamon proved to be
resistant to salinity, Megaritiki and Kothreiki salt-tolerant and Mastoidis,
Amphissis and Koroneiki less tolerant (Chartzoulakis et al., 2002, 2004,
Chartzoulakis, 2005). The degree of susceptibility to salinity stress varied
Mineral Nutrition
205
between the tested cultivars. Tolerance to sea water follows the order:
Kalamon > Chondrolia Chalkidikis > Manzanillo. A decline in tolerance
with age and time of exposure to salt was reported for the cv. Arbequina
(Arags et al., 2005). The effect of six different sea water dilutions (0, 4.3, 8.5,
12.8, 17.1 and 21.3% sea water) on total plant mineral content, leaf number,
leaf area and leaf development, of the cvs Chondrolia Chalkidikis, Kalamon
and Manzanilla de Sevilla was measured (see Tables 18.9 and 18.10).
Plants grown under NaCl salinity show an increase in Na and Cl concentrations and a decrease in plant growth and K, Ca and Mg concentrations
(Vigo et al., 2002). The decrease in K, Ca and Mg concentrations is due to the
antagonistic effect of Na with these three elements. A decrease in leaf B
concentration is also usually observed (Vigo et al., 2002). These results show that
sea water decreases plant growth, increases Na and Cl concentrations in all plant
parts and decreases K, Ca and B concentration (see Tables 18.9 and 18.10).
Table 18.9. The effect of irrigation with six sea water dilutions on total plant
content of P, K, Ca, Mg, Na and Cl (mg) and Fe (g) in the olive cvs Chondrolia
Chalkidikis, Manzanilla de Sevilla and Kalamon (from Vigo et al., 2005).
Sea water dilution (%)
0
4.3
8.5
12.8
17.1
21.3
LSD (5%)
Manzanilla de Sevilla
0
4.3
8.5
12.8
17.1
21.3
LSD (5%)
Kalamon
0
4.3
8.5
12.8
17.1
21.3
LSD (5%)
Ca
Mg
Na
Cl
29.3
32.7
30.2
21.3
20.3
19.3
7.59
200.0
207.2
169.1
118.8
96.7
96.5
48.11
94.8
72.8
65.4
51.9
47.1
49.2
20.86
32.8
35.6
31.9
26.5
23.4
26.6
NS
28.6
73.2
79.4
67.6
68.9
86.2
18.06
60.3
95.1
84.6
67.6
75.8
123.5
28.17
5,721
6,308
3,879
3,262
2,013
3,898
2,598.00
33.6
32.8
27.8
31.4
28.1
18.2
6.85
173.4
129.9
98.8
97.6
84.5
54.4
28.61
104.7
76.4
60.1
55.0
55.0
39.0
15.57
30.4
30.8
26.1
26.8
26.5
19.4
6.88
25.5
73.2
83.6
81.0
92.3
86.0
16.95
43.4
93.9
104.9
100.9
109.4
105.0
25.54
6,584
6,160
35,222
3,555
4,172
2,673
1,622.90
11.5
10.3
11.2
8.7
8.3
5.9
NS
78.9
56.8
70.6
51.8
52.6
42.5
NS
50.2
36.3
37.1
31.3
28.7
23.2
13.18
12.2
13.1
15.8
13.0
12.6
10.4
NS
9.8
26.7
39.3
32.4
32.1
30.0
10.34
19.2
27.2
37.0
29.0
30.1
31.4
NS
1,317
1,761
989
745
704
547
600.50
LSD, least signicant difference for P 0.05; NS, not signicant.
Fe
206
Chapter 18
Table 18.10. The effect of irrigation with six sea water dilutions on total leaf
number, nal leaf area and number of days from initiation to nal area of newly
formed leavesa in the olive cvs Chondrolia Chalkidikis (Ch. Ch.) and Manzanilla
de Sevilla (Mz. S.) (from Vigo et al., 2005).
Sea water
dilution (%)
0
4.3
8.5
12.8
17.1
21.3
LSD (5%)
Final leaf
area (cm2)
Time from leaf

initiation to nal
leaf area (days)
Ch. Ch. Mz. S.
Ch. Ch. Mz. S.
Total leaves (n)

Ch. Ch.
23
19
17
16
13
15
3.6
Mz. S.
22
20
19
16
15
14
3.0
4.9
5.3
5.2
4.9
4.7
4.7
NS
4.1
3.8
3.4
3.6
3.1
3.0
0.40
20
20
21
22
22
22
NS
20
20
20
21
21
22
NS
aAverage
of all newly developed leaves on the main shoot during the 157 days of the
experiment.
LSD, least signicant difference for P 0.05; NS, not signicant.
With regard to the distribution of Na and Cl, even at the highest sea water
concentration most of the Na and Cl was found in base stems and roots,
indicating that the plants of the tested cultivars excluded Na and Cl ions from
their leaves. Thus it is clear that salt exclusion from the shoot is the mechanism
of salinity tolerance in olives. Tolerance to sea water of the tested cultivars
seems to be related to total plant K content, K concentration and the K:Na ratio
of leaves. Also, the relatively high Ca concentration of the roots of Kalamon
plays an important role in improving tolerance to salinity. Calcium maintains
the integrity and function of cellular membranes. In that way root selectivity
for K instead of Na is maintained. The increase in leaf thickness as a response to
saline treatment seems to increase the internal surface per unit of leaf area in
which CO2 and water vapour diffusion takes place, so reducing the internal
resistance to CO2 assimilation. The results indicate that, in coastal areas with
light-textured soils, diluted sea water could be used for irrigation of olive plants.
Finally, the correlation coefcients of mineral concentrations with (i) time (see
Table 18.11); and (ii) B concentration (see Table 18.12) are presented.
FERTILIZATION OF OLIVE ORCHARDS

Spatial variability in soil fertility
The most common practice in olive orchards is to apply fertilizer without
taking into consideration the variability in soil fertility (Lpez-Granados et al.,
Mineral Nutrition
207
Table 18.11. Correlation coefcients of mineral concentrations with time,

experimental results (from Chatzissavvidis et al., 2005).
Boron concentration in nutrient solution (mg/l)
Mineral
B
N
P
K
Ca
Mg
Fe
Mn
Zn
*Signicant
0.5
5.0
0.574****
0.600**
NS
0.648**
NS
NS
0.646**
NS
NS
0.498****
0.862**
NS
NS
NS
NS
0.730**
0.551**
NS
0.483**
0.684**
NS
NS
NS
NS
0.655**
0.530**
NS
for P 0.05; **signicant for P 0.01; NS, not signicant.
Table 18.12. Correlation coefcients between B concentration and concentration

of the other elements (Chatzissavvidis et al., 2005).
Boron concentration in nutrient solution (mg/l)
Mineral
N
P
K
Ca
Mg
Fe
Mn
Zn
*Signicant
0.5
5.0
0.593**
NS
NS
0.471
NS
0.632**
0.632**
NS
0.567**
NS
NS
NS
0.457**
0.576**
0.644**
NS
NS
NS
NS
0.736**
NS
NS
NS
NS
for P 0.05; **signicant for P 0.01; NS, not signicant.
2004). This process may lead to under-application or over-application of

nutrients in some olive orchards. As a consequence under-fertilized orchards
do not give the optimum yield, while in over-fertilized sites there is the danger
of pollution with NO3 and also the high fertilization cost (Bouma, 1997).
Therefore, olive growers should be aware of the varying fertility levels of
individual orchard sites and manage their fertilization appropriately.
Nitrogen fertilization should be conducted at the end of winter, supplying
ammonium sulphate or urea to the soil or urea in the form of a foliar spray. For
olive growers, therefore, in order to both protect the environment and increase
their income it is necessary to adopt a strategy that adjusts fertilization
according to the fertility requirements of each olive grove. Hence, N fertilizer is
208
Chapter 18
not supplied when leaf N is greater than a threshold value; such a limit,
according to Fernndez-Escobar et al. (2002), is 1.5%. Nitrogen levels above
this limit adversely affect the polyphenol level of the oil, negatively affecting
the quality of both the oil and the groundwater. The quality of table olives
is also affected by fertilization (Morales-Sillero et al., 2006). Furthermore,
adequate K fertilization increases drought tolerance and adaptation (Restrepo
et al., 2002).
Nutrient uptake by olive

Many olive growers believe that olive plants have low requirements for nutrient
elements, and so under-fertilize olives. Others used to apply fertilizer quantities
in excess of their actual needs. The total N absorption required to cover the
annual needs of growth ranges from 9.2 kg/ha during the second year of
growth to 185.9 kg/ha in the sixth. The respective quantities for K2O are
8.6163.1 kg/ha, and for P 1.330.7 kg/ha.
The N, P, and K requirements (percentage of the total quantity) during the
stages of fruit set, pit hardening and maturation are given in Table 18.13.
Estimation of fertilizer requirements

The optimal rate of fertilizer application to olives is that which results in the
maximum income. This rate is smaller than the fertilizer quantity required to
produce the maximum yield. The most important way to increase olive
production within a particular olive culture is to recognize the nutrient
deciencies and to apply the required quantities of selected fertilizers.
Therefore, a relationship should be established between the rate of fertilizer and
yield. In order to do this, experimentation should be planned to give the
required information.
This relationship could be expressed by the equation G=f(N), where G is
the prot from the product and f(N) is the rate of the application of one fertilizer
(N=nitrogen, P=phosphorus, K=potassium). The optimal rate is calculated by
solving the equation dG/dN=0, where N is the amount of N producing the
Table 18.13. N, P and K requirements of olive trees during three important stages
in the fruiting process.
Nutrient
N
P
K
Fruit set (%)
Pit hardening (%)
Maturation (%)
39.5
24.6
33.5
28.5
38.9
31.4
32.0
36.5
35.1
Mineral Nutrition
209
maximum prot and dG is the differential equation indicating prot increase

per unit nitrogen, dN. For concurrent application of two or more nutrients the
equation becomes G=f(N, P, K), where N, P and K are the optimal rates of
nitrogen, phosphorus and potassium. The optimal rates are calculated by
solving the equation dG/dN=dG/dP=dG/dK.
Foliar application of urea to olive

With the exception of N, macronutrients cannot be applied to fruit trees foliarly.
Urea contains a high percentage (46%) of N. Furthermore, uptake of urea is
rapid after its application. When conditions are favourable 6070% of the urea
applied can be absorbed by the olive leaves. When foliar application is
conducted during October the N content of the olive leaves increases by 47%, is
subsequently reduced over the next 6 months and redistributed within the
plants in order to effect various physiological processes. Therefore, accurate
timing of foliar fertilization is important in order to effect certain processes.
The use of 15N-labelled urea allows the measurement of redistribution of N
within the plant (Weinbaum, 1984). Foliar uptake of urea is not inuenced by
leaf N status. Translocation of urea N from mature leaves to vegetative tissues
in N-decient plants, however, is lower than that in N-sufcient plants. Foliageapplied urea is translocated acropetally and basipetally. Translocation of
foliage-applied urea N to roots of N-decient plants is less affected than
translocation to shoots.
19
GROWTH AND SALT TOLERANCE
OF THE OLIVE
INTRODUCTION
Salinization of lands is increasing progressively throughout the world. It is
estimated that approximately one-third of the irrigated lands on a worldwide
basis and one-half of the irrigated lands in semi-arid regions are salinized. It is
also estimated that 10 million ha of these irrigated lands are taken out of
production due to high salinity (Epstein et al., 1980). Of the total land area
cultivated, about 5% (Munns, 1993) is affected by salt, and therefore, it is
important to study salinity and to measure the salt tolerance of the various
existing cultivars.
With respect to olives there is not enough information available. Therefore,
this chapter presents the available knowledge concerning the behaviour of
olives to salinity.
PLANT GROWTH, YIELD AND OIL QUALITY

Plant growth of olives, i.e. dry weight, shoot length, total leaf area and root
length, is reduced by moderate and high levels of salinity (Therios and
Misopolinos, 1988). The amount of growth reduction is a function of the
cultivar, salt type and its concentration and the length of exposure of plants to
the salt. In the olive, leaves and their total area are more affected by salts than
other plant parts. Salinity above a critical limit leads to stomatal closure,
smaller leaf size, reduction in the number of perfect owers per inorescence
and also pollen viability. Furthermore, the top:root ratio is reduced.
The effect of salinization on pollen characteristics results in a signicant
reduction of viability, germination capacity and fruit set (Cresti et al., 1994).
High salinity reduces the yield of olive trees (Gucci and Tattini, 1997); high
salinity and brackish water reduce yield and oil percentage in most cases.
However, some authors (Bouaziz, 1990) report that irrigation with brackish
water did not affect yield and oil percentage and did not cause alternate
211
212
Chapter 19
bearing. Klein et al. (1992) reported for electrical conductivity (Ec) values up to
4.2 dS/m an increase or decrease in the yield observed, this being a function of
planting density. However, when the Ec of the irrigation water was 7.5 dS/m oil
yield and total fresh weight of fruits declined by 6080% in comparison with
the control irrigated by good-quality water. Other authors report that saline
reduces oil content and that this decrease is cultivar dependent (Chartzoulakis
et al., 2004; Stefanoudaki, 2004).
Salinity stress affects olive oil composition by an acceleration of ripening,
and its effects can be both positive and negative (Cresti et al., 1994). The
linoleic:oleic acid ratio sharply increases in the oil during the growing season,
as the degree of unsaturation increases with the fruit ripening. Also, the
activity of linoleic acid desaturase is strongly inhibited under saline conditions.
Another effect is the increase of aliphatic and triterpenic alcohols, as a
consequence of accelerated ripening.
Salinity results in increased phenol concentration in the oil (Stefanoudaki,
2004), and especially in the second fraction of secoiridoid derivatives. With
regard to negative effects the increase in total saturated fatty acids and
especially that of palmitic acid was included (Zarrouk et al., 1996;
Stefanoudaki, 2004). Furthermore, oleic acid decreases or linoleic acid
increases, and the ratio of oleic:linoleic acid decreases.
WATER RELATIONS AND PHOTOSYNTHESIS

The effect of salinity can be divided into osmotic and toxic components. Due to
the osmotic effect water absorption is reduced as salinity increases (Therios
and Misopolinos, 1988). Upon exposure to salinity leaf water potential (leaf)
and relative water content (RWC) are reduced. The decrease in is followed by
a decrease in osmotic potential (s). It is important to understand that when
salinized olive plants at levels of up to 100 mM NaCl are exposed again to nonsalinized solutions the plants completely recover and start growing again. The
olive plant has, therefore, the ability to adjust osmotically. Osmotic adjustment
is attained either by ion accumulation in the vacuoles or through soluble
carbohydrates. The most common osmoregulatory substance in olives is
mannitol.
In experiments with the cultivars Leccino and Frantoio, cations
contributed about 40% of the total , while the contribution of mannitol and
glucose was about 30%. An analysis of the water relation characteristics of
olive leaves under salt stress for the salt-tolerant Frantoio and salt-sensitive
Leccino was reported by Gucci et al. (1997a). The osmotic contribution of Na
was 0.12.1% in the controls and 1520% for the 200 mM salt-treated plants
(Gucci et al., 1997a).
When searching the literature on the subject of photosynthesis, all
published papers to date show that Pn is reduced under salinity. The initial Pn
Growth and Salt Tolerance
213
value of about 1415 mol is signicantly reduced. The rate of decrease of Pn

depends on the salt tolerance of the olive cultivar; this decrease varies from
20% for the salt-tolerant olive cv. Kalamon up to 62% for the cv. Amphissis,
which is moderately sensitive (Chartzoulakis et al., 2002). This reduction in Pn
could be ascribed to Na and Cl accumulation in the leaves under salt stress
(toxic effect), and also to the decrease in mesophyll conductance due to
increased leaf thickness (Syvertsen et al., 1995).
Salinity has a negative effect on photosynthesis (Tattini et al., 1995, 1997,
1999; Vigo et al., 2002; Centritto et al., 2003; Loreto et al., 2003; Chartzoulakis
et al., 2004; Chartzoulakis, 2005; Tabatabaei, 2006). Salinity stress brought
about a reduction in net CO2 assimilation and stomatal conductance in two
cultivars (Tattini et al., 1997), but the effect was more pronounced in the salttolerant cv. Frantoio than in the salt-sensitive Leccino. Hence, gas exchange
parameters may be misleading if used to evaluate the salt tolerance of olive
genotypes. Furthermore, upon relief of NaCl stress the recovery in gas exchange
parameters was slower in the salt-sensitive cultivar.
ION CONTENT OF VARIOUS OLIVE TISSUES

The effect of salinity on nutrient content is evidenced by the increase or
decrease in the concentration of certain micro- and macronutrients. Hence,
increases in Cl and Na+ concentrations in olive tissues occur with increasing
NaCl in the soil (Tattini et al., 1992). The most tolerant cultivars prevent the
entry or translocation of Na and Cl from the roots to the parts above ground
level. Therefore, salt-tolerant cultivars compartmentalize the toxic ions to root
or trunk tissues. When the concentrations of NaCl are moderate, olive cultivars
activate an Na+ exclusion mechanism.
However, when the concentration of salts in the soil solution is high,
sodium is transported to the tops, especially in salt-sensitive cultivars. Thus,
toxicity symptoms appear in the form of peripheral necrosis of leaves. The
opposite is true for the salt-tolerant cultivars, which translocate less sodium to
tops. Hence, tolerant cultivars such as the Greek cv. Kalamon and the Italian
Frantoio have more efcient mechanisms of inhibiting Na+ transport to leaves
or effecting Na+ exclusion or retention by the roots.
The effect of salinity on P absorption depends on various factors such as
plant species, cultivar, stage of growth, salt concentration and the ionic form of
P in the substrate. In many cases salinity reduces P concentration (Sharpley et
al., 1992), while in other cultivars P concentration remains constant.
Decreased P concentration, as a result of salinity, was observed in experiments
conducted in the soil. The K+ concentration of leaves was reduced when the
NaCl of the nutrient solution was increased (Janzen and Chang, 1987).
Furthermore, the K+ content of leaves in four olive cvs (Amygdalolia,
Adramittini, Manzanillo and Frantoio) was decreased by increasing the
214
Chapter 19
NaCl concentration in the nutrient solution (Therios and Karagiannidis,

1991) due to K+Na+ antagonism. The highest K+ decrease occurs in roots and
old leaves, while young olive leaves have higher K+ concentrations, which may
help osmoregulation in the plant.
Calcium plays many important roles in olive nutrition: cell division, cell
wall formation, protein synthesis, competition with other elements and
selectivity and semipermeability of membranes. Calcium competes with other
cations like Na+ (Marschner, 1997). Calcium is a key element in limiting the
toxic effects of Na+, increasing the Ca2+:Na+ ratio in the external solution,
which alleviates the toxic symptoms of NaCl (Rinaldelli and Mancuso, 1994).
Calcium exerts a positive role concerning salt tolerance. This effect is
manifested in both the trans-membrane and trans-root potentials. The
presence of Ca2+ considerably limits the decrease in extra- and intracellular
resistance due to Na+. Therefore, the function of Ca is protective with respect to
membrane integrity by both maintenance of adequate Ca2+ binding to the
plasma membrane and prevention of K+ leakage (Cramer et al., 1985;
Mancuso and Rinaldelli, 1996).
Salinity can be reduced by soil reclamation and drainage, but the cost is
high. Therefore, a more cost-effective approach is to add Ca2+ to the saline
growth medium. Addition of calcium sulphate maintains membrane
permeability and selectivity. Salinity stress has been shown to induce transient
Ca2+ inux into the cell cytoplasm. Therefore, calcium channels responsible for
Ca2+ inux may represent one type of sensor to these stress signals. Increases in
cytosolic Ca2+ are perceived by various Ca2+-binding proteins such as Ca2+dependent protein kinases and the SOS3 family of Ca2+ sensors. Genetic
analysis indicates that the salt-overly-sensitive (SOS) mutants SOS1, SOS2 and
SOS3 function in a common pathway in controlling salt tolerance.
CYTOKININS AND THE RESPONSE TO SALINITY STRESS

Cytokinins (CKs) are involved in the regulation of many aspects of growth and
differentiation, such as nutrient mobilization and apical dominance (Hare et
al., 1997). Furthermore, CKs are involved in responses to adverse conditions,
like salinity. Plant hormones play a role in the transformation of stress signals
into the gene expression necessary to effect adaptation to marginal
environmental conditions. Among the growth regulators linked to responses
to abiotic stresses ABA and ethylene are included. Cytokinins are antagonists
of ABA in many processes such as stomatal opening, and in plant responses to
high salinity. Among the growth regulators ABA and CKs are likely to play a
crucial role in controlling the responses of plants to stressful conditions of the
environment.
215
Endogenous and exogenous cytokinins and their effects on

stress responses
Under eld conditions water decit is produced by water deprivation and soil
salinity. Studies with root exudates indicate that, when sunower plants were
exposed to water stress, their root exudates contained less kinetin-like activity
substances. In leaves of tomato plants during the rst 2 days after the initiation
of salt stress, CKs increased and this was followed by a depression in
concentration afterwards. Furthermore, the levels of CKs in the shoots of
sunower plants affected by salinity were almost half of those of the unstressed
plants. The decrease in CK content is an early response to salt stress, and the
effects of NaCl on salt-sensitive varieties are not mediated by CKs. The
exogenous application of kinetin alleviated the salinity stress effects seen
during the growth of wheat seedlings. The CK thidiazuron (phenylurea-type
CK) signicantly increased the yield of salinity-stressed wheat.
Mineral deciency, salinization and cytokinin levels

Salinity reduces mineral availability and this signicantly affects CK levels in
vivo. Salt stress lowers N levels in plants and leads to a decrease in CK levels
(Darral and Wareing, 1981). The same is true for P deciency and CK levels.
When CKs are applied exogenously in N-decient plants growth is stimulated,
indicating that CKs change the nutrient supply, especially of N. The symptoms
of Ca2+ deciency are delayed by benzyladenine (BA) application.
The effect of salinity on cytokinin metabolism and transport

Cytokinin metabolism is a dynamic process between CK biosynthesis and
catabolism, resulting in loss of biological activity. Due to the existence of at least 20
natural CKs in plants it is not easy to evaluate the changes in CK levels under
salinity stress conditions. In fact there is not enough information on olives about
the pathway of CK biosynthesis and the enzymes which regulate certain metabolic
steps. The activity of CKs can be reduced by processes leading to their inactivation
via the formation of CK nucleosides or N-glycosylation, conjugation or oxidation.
The enzyme which degrades CKs is CK oxidase. The reversible conjugation of CKs
is a possible mechanism by which olive plants achieve hormonal homeostasis and
CK activity under adverse conditions. CKs are considered to be transported from
the roots to the shoots via the xylem and transpiration stream, indicating the
mechanism by which the root affects shoot physiology.
Many CK effects have long been known to be mediated via stimulation of
ethylene production. Ethylene is induced by many stress factors such as
drought and salinity. Cytokinin-mediated stimulation of ethylene production
216
Chapter 19
appears to be of particular importance during water and salt stress. Apart from
ethylene, salicylic acid (SA) and jasmonic acid (JA) are also important signal
molecules involved in the activation of plant deciency responses to salinity
(Sano et al., 1996). Cytokinin is required for the biosynthesis of SA and JA,
which subsequently serve as endogenous inducers.
SALINITY AND OXIDATIVE STRESS

Salinity involves both an osmotic and a toxic effect of salts. Furthermore,
salinity generates oxidative stress, thus increasing the level of reactive oxygen
species (ROS) such as superoxide radical (O2), hydroxyl radical (OH) and
hydrogen peroxide (H2O2), all of which damage membranes, proteins, DNA
and lipids (Fadzilla et al., 1997).
Determination of the malondialdehyde (MDA) content, and therefore the
extent of membrane lipid peroxidation, is used to assess the degree of plant
sensitivity to oxidative damage. Lipid peroxidation remains unchanged in
plants tolerant to salinity and drought.
SALINITY AND MANNITOL CONTENT

In olive pulp, apart from the other components, concentrations of up to 8 mg/g
d.m. of mannitol have been recorded (Conde et al., 2007). Mannitol is a polyol or
sugar alcohol. In some plant species polyols are products of photosynthesis of
mature leaves. Mannitol is present in more than 100 species. The most common
families include the following: Rubiaceae (coffee), Oleaceae and Apiaceae.
Mannitol is produced in leaves from mannose 6-phosphate via the activities of
NADH-dependent mannose 6-phosphate reductase and mannitol 6-phosphate
phosphatase.
Mannitol production leads to more efcient carbon use and confers salt
tolerance. Mannitol content increases as the salinity of the growth medium
increases. Addition of 100500 mM NaCl to cultivated olive cells enhances the
capacity of the polyolH+ symport system. Furthermore, cell viability is greater
in mannitol-grown cells after a 250 or 500 mM NaCl pulse, while sucrosegrown olive cells have reduced viability.
TREATMENT OF NUTRIENT SOLUTIONS OR IRRIGATION

WATER WITH RADIO WAVES IN THE REDUCTION OF SALT
TOXICITY
A new approach to decreasing the negative effects of salinity of irrigation water
is by changing the characteristics of the nutrient solution or the irrigation
217
water. This is accomplished by using an electromagnetic eld to which the

nutrient solution or the irrigation water is exposed before its use. Experiments
in hydroponic culture of tomatoes indicate that the electromagnetic eld
improves the solubility of salts and the total yield (Roberts, 2002). The
equipment used produces low-frequency radio waves, i.e. 2106 vibrations/s,
which breaks the salts down to a size of 24 . This treatment does not modify
the chemical composition, and offers the following advantages:
Use of brackish or saline water for irrigation (Bouaziz, 1990; Benlloch et

al., 1991; Briccoli Bati et al., 1994; Fodale et al., 2006).
Decrease in salt toxicity.
Improvement in fruit size and yield.
Data from the cv. Chondrolia Chalkidikis indicate that, even with an Ec of
30 dS/m, plants continue to grow and no symptoms of toxicity appear (Ioannis
Therios, unpublished data). The number of leaves is signicantly greater with
the use of the electronic equipment. Potassium concentration is constant at all
Ec values with the use of the equipment and decreases without it.
Furthermore, Ca concentration is signicantly greater with this method, but
lower and decreased with increasing Ec. Phosphorus follows the same pattern
as Ca.
Another way to reduce Na accumulation in olive leaves and to restore K
levels similar to those of controls is the inclusion of 0.1100 mM K in the
nutrient solution. It has been suggested that K+ is one of the osmolytes, and its
accumulation in plant cells might facilitate osmotic adjustment, lower the
internal osmotic potential and contribute to salt tolerance. However addition
of K+ to the irrigation water did not improve Pn in olives compared with plants
receiving the same salinity treatment but with no added K+.
SALT TOLERANCE
Olive is a moderately salt-tolerant plant (Rugini and Fedeli, 1990). At Ec values
of 46 dS/m olive growth is reduced by 10%. Therios and Misopolinos (1988)
found that 3-year-old olive plants could be grown without problems at NaCl
concentrations < 80 mM (Ecw of 8.0 dS/m) during a 3-month period (see Fig.
19.1). Further studies indicated that olives can even do well under higher NaCl
concentrations: experiments were conducted with NaCl concentrations of up
to 150 mM NaCl. In another experiment olive trees were irrigated with
Hoagland nutrient solution diluted with sea water (Ec of sea water = 58 dS/m);
the maximum rate of dilution was 23% and its Ec value was 13 dS/m (Vigo et
al., 2005). Furthermore, the type of salt in the irrigation water affects the
severity of the toxicity symptoms. In one experiment the salts NaCl, CaCl2 and
KCl were tested. From these, at iso-osmotic concentrations, the most toxic was
KCl, reducing growth by a far greater extent than the other two (Vigo, 1999;
218
Chapter 19
Fig. 19.1. Effects of NaCl (080 me/l) concentrations on shoot growth 1 (a) and
4 (b) months into an experimental trial on four olive cultivars (from Therios and
Misopolinos, 1988).
Vigo et al., 2002). Furthermore, other authors (Bartolini et al., 1991) found
that Na2SO4 was more toxic than NaCl when the cv. Maurino was used. Salt
toxicity, of course, is a function of the olive cultivar and there is signicant
genotypic variation.
Various authors provide more information on the classication of salt
tolerance in olive cultivars (Therios and Misopolinos, 1988; Bouaziz, 1990;
Tattini et al., 1992, 1994; Benlloch et al., 1994; Marin et al., 1995; Gucci and
Tattini, 1997; Vigo, 1999; Chartzoulakis et al., 2002; Troncoso et al., 2004;
Arags et al., 2005; Chartzoulakis, 2005; Demiral, 2005). Chartzoulakis
219
(2005) classied these data in the form of a table giving the resistance of
cultivars, their origin and the source of the literature. Olive cultivars are
classied as salt tolerant, semi-tolerant or sensitive.
Tolerant: Megaritiki, Lianolia Kerkiras, Kalamon, Kothreiki, Frantoio,

Arbequina, Picual, Lechin de Sevilla, Hamed and Chemlali.
Semi-tolerant: Amphissis, Koroneiki, Mastoides, Valanolia, Adramittini,
Coratina, Moraiolo and Maurino.
Sensitive: Chondrolia Chalkidikis, Throubolia, Leccino, Nabal, Chetoui,
Calego and Meski.
Salt tolerance in olives could be ascribed to certain mechanisms (Tattini et

al., 1994; Amane et al., 1999; Al-Absi et al., 2003), such as retention of Na or
Cl by the roots and stem (Tattini et al., 1994) or ion exclusion due to K
selectivity instead of Na. Another mechanism is compartmentation of toxic
ions. Salt-tolerant cultivars restrict the ingress of toxic ions such as Na or Cl
into vacuoles and thus avoid toxicity (Loreto and Bongi, 1987).
FUTURE GOALS
One widely used approach to unravel olive salt-tolerance mechanisms has been
to identify cellular processes and genes whose activity or expression is
regulated by salt stress (Watad et al., 1991; Hasegawa et al., 2000). The
assumption is that salt-regulated processes and genes probably function in salt
tolerance. Changes in cellular activities in response to salt stress include cell
wall alteration, decline in photosynthesis (Locy et al., 1996), potassium
content (Rains, 1972) and increases in Na+ and organic solutes (proline,
mannitol, etc.) (Greenway and Munns, 1980). Another approach is to identify
genes and cellular processes that are crucial for olive salt tolerance. Research
with the species Arabidopsis resulted in the isolation of several salt-overly
sensitive (SOS) mutants such as SOS1 (Wu et al., 1996), which is defective in
high-afnity K uptake. This means that K uptake is essential for salt tolerance
in glycophytes (Wu et al., 1996) under NaCl stress. SOS1 mutants accumulate
more proline (Liu and Zhu, 1997) and absorb less Na+ and K+.
The SOS2 mutants are specically hypersensitive to inhibition by Na+ or
+
Li and not hypersensitive to general osmotic stress. Furthermore, SOS2
mutants are necessary for K+ nutrition, since they do not grow in a culture
medium with low K+ concentration. Therefore, SOS1, SOS2 and SOS3 are
postulated to encode regulatory components controlling plant K+ nutrition
that, in turn, are essential for salt tolerance.
Some questions which should be asked include:
1. Do genes similar to SOS1, SOS2 and SOS3 exist?
2. Is salt sensitivity in the olive correlated with cellular Na+ content?
220
Chapter 19
3. Do genes exist in olives encoding for: (i) the enzyme PCP synthetase involved
in proline biosynthesis; (ii) a protein with a potential protective function during
desiccation (Yamaguchi-Shinozaki and Shinozaki, 1993); and (iii) a gene of cold
tolerance, drought and salt stress (MYB)-related transcription factor and
phospholipase that plays a role in osmotic signal transduction (Hirayama et al.,
1995)?
4. Which is more detrimental to plants under salt stress: higher tissue Na+ or
lower K+?
5. Does the level of salt tolerance, as measured by root growth, correlate
closely with K+ content?
6. The accumulation of compatible osmolytes is often considered to be a
universal protective mechanism used by many plants under salt stress. Is
proline accumulation an adaptive process or a response to salt stress injury?
This is an open question.
20
PRUNING
WHAT IS PRUNING?
Pruning of olive trees has two main objectives, to improve vegetative growth
and reproductive growth. The term pruning includes the techniques known as
cutting, heading, incision, inclination, twisting and girdling. Successful pruning
involves the knowledge and experience of olive tree physiology; the response of
trees to pruning varies with the age of the tree, vigour and cultivar, and it is an
excellent and very efcient means of controlling plant growth and productivity.
In pruning, by cutting certain parts of the canopy, the growth of the remaining
parts is stimulated; cutting is separated into the categories of thinning and
heading. When we undertake thinning we eliminate shoots from their base.
With such removal the entire shoots stimulate the vigour of the remaining
shoots by reducing competition for light, nutrients and water. An excellent
analysis of pruning and training systems for olive trees can be found in Gucci
and Cantini (2004b). By the term heading we mean the cutting back of a
young shoot to a bud or to shorten an old shoot to a lateral branch. With
heading, the growth of shoots close to the cut is promoted.
WHY PRUNE?
Pruning is an important agricultural task. There are many reasons why, and
circumstances when, olive trees should be pruned.
Rapid development of tree skeleton and achievement of a balance between

structure and productivity, and optimum light penetration.
Achievement of a strong tree skeleton necessary to support fruit load and
able to transmit the vibrations from mechanical harvesters.
To achieve early onset of production and high yields.
To renew the canopy in old, non-productive trees to stimulate productivity,
(Cimato et al., 1990).
221
222
Chapter 20
To create new canopy after the olive tree has been damaged by frost, re,
pests or disease.
To give the appropriate shape to the olive canopy and to adjust the shape of
the tree canopy for the dense and super-high-density planting systems.
To improve the quality of olive fruit and oil.
RULES OF PRUNING
The style of pruning depends on olive tree age, crop load, the use of olives as
table or olive oil fruits, soil fertility, environmental conditions and fruit load.
Although these factors differ from one area to another and from cultivar to
cultivar, certain general rules are applicable to pruning.
It is not necessary to prune olive trees every year; in some cases pruning is
conducted every 2 years. Therefore, pruning cost is a very important factor.
Plant age is a determining factor of the type of pruning (light, medium,
severe).
The pruning method should be simple and fast; we can then check light
penetration into the canopy and assess the need for more severe pruning.
Pruning starts from the top of the tree and proceeds towards the base.
Large shoots are cut rst, followed by those of smaller diameter.
In mature plants pruning is light; pruning intensity increases with age of
plant.
The cut should be executed close to the point of attachment of the lateral
branch.
APICAL DOMINANCE
Growth in olives is controlled by endogenous hormones, and pruning can
modify the regular growth pattern by changing the hormone balance. Auxin
(IAA) is produced in the upper shoot tips, and in young leaves it is transported
downward via the phloem and exerts a certain degree of control on shoots,
buds and branches lying below the shoot tips. The large amount of IAA
produced and transported downwards affects the growth of shoots by the
following means:
Inhibition of lateral bud growth.

Decrease in growth rate in length of lateral branches.
Increase in the angle between side branches and trunk.
These growth characteristics are linked to the supply of IAA and can be
modied by pruning, girdling or hormone application. The movement of auxin
Pruning
223
and its asymmetric distribution affects lateral bud inhibition, cambial activity
and stem elongation. The same endogenous concentration of auxin has varying
effects depending on the plant tissue, i.e. it inhibits growth of lateral buds and
stimulates cell division in the cambium. Therefore, when IAA produced by a
vigourous stem moves to the base of the stem, this results in a wider crotch angle.
EFFECTS OF PRUNING
Photosynthesis
The rate of CO2 assimilation per unit area of leaf is an important characteristic
of higher plants and integrates all the biochemical and biophysical processes.
Pruning techniques lead to removal and reduction of photosynthetic area. This
decreases the efciency of carbon assimilation and also the vigour of the
remaining shoots. Large decreases in leaf area by pruning negatively affect
olive tree growth. On the contrary, absence of pruning or very light pruning
has, as a consequence, the shading of the olive canopy.
Shading delays fruit ripening and decreases olive oil production. Due to
lack of pruning, in a dense canopy light penetration is sometimes very low and
less than 10% of sunlight intensity. Due to shading, ower bud differentiation
and fruit set can be low and this effect is a function of cultivar, duration of
shading and time of year. Hence, shading from July to October decreases ower
bud formation (Tombesi and Cartechini, 1986). Photosynthesis requires open
stomata, and therefore CO2 assimilation is concurrent with transpiration,
leading to water loss. This means that pruning removes a proportion of leaf
area and it reduces water consumption and improves water use efciency.
Shape of the olive plant and its growth habit

When olive plants remain unpruned they retain a central leader, and their shape
is spherical, hemispherical or ellipsoidal. The shape of the olive tree can be
explained by the growth habit, which is basitonic. Therefore, the lateral shoots
closer to the apex grow more than shoots located on the lower part of a branch. In
addition, differences in canopy shape also arise from differences between cultivars.
Shoot length and pruning

The shortening of shoots should not be excessive, since production will be
reduced, on the contrary, if the roots are not shortened, they are going to
overproduce and nally become exhausted. A practical means of determining the
224
Chapter 20
physiological condition of the current years growth of fruiting shoots is their

length. Shoots < 20 cm are inadequate and pruning is necessary to increase
their length. Shoots > 60 cm are considered very vigourous, but their
productivity is unreliable. The best shoot length varies between 20 and 60 cm;
such shoots produce the highest yields.
PRUNING AND HORMONES

Growth in olives is controlled by endogenous hormones, and pruning can alter
the growth pattern by modifying the hormone balance. The auxin IAA is
produced in vigourous shoot tips and in young leaves. Afterwards, it moves
downwards in the phloem and modies the growth habit of shoots, buds and
branches below the point of auxin production (Westwood, 1978). The auxin
thus transported affects the olive tree in the following ways:
Inhibition of lateral bud growth.

Depression of elongation of lateral branches.
The angle between the trunk and lateral branches is increased.
These effects depend on the natural auxin supply, which can be modied
by pruning or hormone application. Decapitated shoots treated with
2501000 ppm of indolebutyric acid (IBA) resulted in wide branch angles of
all the shoots below that level. In contrast, girdling stopped the downward
movement of auxin and gave rise to narrow-angled branches.
PRUNING TOOLS
Essential tools are pruning shears, handsaw, chainsaw and gloves. Of these the
handsaw is an efcient tool for cutting shoots or branches of diameter 3080
cm. The chainsaw has to be light in weight, thus reducing the pruning time
and its cost. Furthermore, pneumatic tools may be used. All tools should be
clean and sharp and must be disinfected, especially if there is any possibility of
the presence of Pseudomonas syringae pv. savastanoi. When trees are thus
infected they should be sprayed foliarly with Cu or other pesticides. Large cuts
should be protected by covering with pruning pastes or paints.
PRUNING AND TREE AGE

Pruning costs
Pruning represents 2030% of the annual cultivation cost of olives. When
employing mechanical pruning that cost is reduced to 7%. However, this type
Pruning
225
of pruning has certain disadvantages. The high cost of hand pruning is

ascribed to the high cost of labour and the signicant amount of time required
per tree. The developing trend today is to reduce costs by reducing pruning to a
minimum.
Pruning during the training period

The objectives of pruning during the training period are set out below:
To create trees of good shape.

To obtain heavy crops and to bring trees into production as early as possible.
To achieve maximum light penetration into the canopy.
This can be managed with trees that have the following qualities:
Appropriate tree size and good sanitary conditions.

Freedom from pests such as Prays oleae, Saissetia oleae, Spilocaea oleaginea
and Verticillium dahliae. The best size for pruning is 70120 cm in height;
smaller plants also can be used, but with no single trunk.
The following guidelines are applicable to the pruning of young trees

(Gucci and Cantini, 2004):
Avoid shortening of shoots, since this promotes the emergence of many

and vigourous shoots close to the cutting surface.
Shoot shortening should be achieved by cutting next to a lateral shoot.
Sucker removal should be carried out during the summer, and also removal
of very vigourous shoots, which antagonize the growth of other shoots.
Pruning during canopy training should be light and be restricted only to
elimination of watersprouts and suckers.
Pruning of mature olive trees

The following procedures should be followed for the annual pruning of mature
olive trees (Sibbett, 1994):
Removal of all broken shoots and branches, suckers and watersprouts, and
vigourous and very weak shoots.
Reduction of the tree to optimal height.
Removal of shoots from the upper part of the canopy to allow better light
penetration.
Reduction of the length of secondary branches.
Annual pruning of table olive cultivars.
Pruning of mature trees varies according to their training system.
226
Chapter 20
Rejuvenation of old trees

The olive tree is characterized by its longevity, since it has the ability to create
new shoots from the various parts of its wood following frost damage.
Furthermore, those trees very old and low in productivity can be rejuvenated.
After de-topping, new, vigourous shoots develop and some of these are selected
to create the new tree canopy, which will start to produce after no more than 3
years. In the case of frost or re damage we wait for the tree to react and
produce new shoots; from the new shoots we select those which will form the
tree canopy and cut off all the dead parts.
PRUNING SHAPES
The main canopy shapes created by pruning of olive trees are the following:
The vase shape, common in Greece, France and Italy.

The spherical shape (trees with central leader), which is not so common
since adequate light penetration is not allowed.
The free palmette, which is not used extensively.
The non-trunk tree, as found in Tunisia or with short trunk (vasebush).
The double- or triple-trunk shape, common in Seville, Spain.
The monoconical shape, i.e. the tree has one trunk and the canopy has a
conical shape.
The multiconical shape, in which each branch has a conical shape;
common in certain parts of Italy.
The two-branched shape; common in Andalucia (Spain) and appropriate
for table olives.
The candlestick shape, as found in Tunisia.
MECHANICAL PRUNING
Nowadays, it is difcult to nd persons with the necessary skill to prune olive
trees. Until recent times mechanization in olive pruning has been rare
(Giametta, 1988; Giametta and Zimbalatti, 1993, 1997; Ferguson et al., 1994,
2002). In the near future, due to increasing costs, mechanical pruning will be
the only viable alternative. Mechanical pruning requires a labour input of 4
h/100 trees in comparison with 128 h/100 trees for hand-pruning.
Pruning machinery generally has a linear arrangement of ve circular saws
(diameter 55 cm) rotating at a speed of 22002300 revs/min. The blades cut the
external edge of the olive canopy and the tractor works at low speed. The blades
cut horizontally at the top of the canopy and vertically or with inclination at the
Pruning
227
edges. The shapes of canopies obtained are cones or pyramids. Mechanical

pruning can result in 80% lower cost of pruning. However, mechanical pruning
has certain disadvantages, since the cutting is indiscriminate and olive trees
develop the appearance of thick, regular hedges of low productivity.
Pruning intensity depends on various factors, including cultivar, age, fruit
load and soil/climatic conditions. The pruning is more severe in trees of low
vigour, such as very old trees. When the trees carry a high fruit load the
previous years shoot growth is reduced. Therefore, pruning the following year
should be lighter. Only watersprouts and problematic shoots should be
removed.
Pruning intensity can be determined based on the growers experience;
growers observe the vegetative reaction of their trees the year following
pruning. One good criterion is the length of annual shoots 2 m above ground
level: shoots shorter than 15 cm indicate that the tree requires more severe
pruning the following winter or spring. In contrast, too many watersprouts on
old wood, or suckers from the trunk base and very long annual shoots, indicate
that the next seasons pruning should be very light.
TIMES AND FREQUENCY OF PRUNING

The best time for olive pruning is between the end of winter and the initiation
of owering, in order to avoid frost problems. Of course, in mild climates
pruning can be started in winter and performed after harvesting. Early
pruning expands the pruning period and thus spreads the timescale for input of
labour. Late pruning, when inorescences appear, is not recommended, since
assimilate reserves are lost along with the cut shoots. It is possible to complete
pruning in summer by removing suckers and watersprouts, as soon as they are
tender. Also, with summer pruning we can correct any problems of tree shape.
Furthermore, summer pruning improves the table olive quality, by reducing
crop load.
In most cases olive trees are pruned every year. Pruning every year is
necessary in table olive cultivars or when the annual shoot length is < 20 cm
due to excessive fruit load, water stress or tree ageing. If there is no problem
with the trees, pruning can be conducted every 2 years or even less frequently.
However, pruning intervals of more than 3 years are not appropriate. As a rule
we say that trees and training systems with a strong skeleton need annual
pruning, while those with a free canopy may be pruned less frequently.
21
OLIVE RIPENING
INTRODUCTION
Olives are grown for one of two purposes: olive oil extraction and green mature
olives for processing. The olive fruit is a drupe and the ripening period is
determined by the time elapsing between the rst purple spots and the peel
turning black. By green maturation we mean the changes in fruit colour and
characteristics during the period the olives are green.
Olive growers have established certain harvesting dates and other criteria
such as fruit colour and fruit drop (Snobar, 1978). These criteria are used by
growers as indices in order to determine the initiation of harvesting. However,
in order to determine the optimum time for harvesting other parameters are
also of value, such as oil content, fruit detachment force and fruit colour.
ASSIMILATE SUPPLY AND OLIVE FRUIT GROWTH

The developing fruit is a strong sink that requires a continuous supply of
building materials. Fruit competes with shoot growth for newly assimilated
materials, as well as for reserves accumulated in different tissues. The leaf:fruit
ratio plays a signicant role in fruit growth and photosynthesis (Proietti, 2003;
Proietti et al., 2006). Most assimilates are supplied by the leaves on the same
shoot where fruits are located, and light and shading affect fruit growth and
composition (Tombesi et al., 1999), and oil synthesis (Proietti et al., 1994). The
dry matter of the endocarp increases by the end of August, and afterwards it
remains constant. Endocarp grows faster than mesocarp until the end of
August, and afterwards its rate slows (Rapoport et al., 1990). Oil synthesis starts
after 60 days of full bloom. The availability of large quantities of assimilates in
July affects mostly endocarp growth. In the period AugustNovember it affects
mesocarp growth and oil synthesis, which is of cytoplasmic origin (Rangel et al.,
1997). Variations in fruit load create differences in assimilate availability and
inuence fruit ripening. Changes in the availability of assimilates at different
229
230
Chapter 21
times during fruit development cause variations in assimilate distribution

between different components of the fruit. During ripening the colour of fruit
skin and esh changes from green to reddish, red or bluish black and the skin
becomes glossy. During fruit ripening the seasonal changes occurring in fruit
size and chemical composition are described in the following section.
SEASONAL CHANGES IN FRUIT DEVELOPMENT

Fruit growth measured as fresh fruit weight differs for each cultivar and is
probably genetically determined. During maturation fruit weight increases,
with the exception of the nal harvesting date, where fruit weight decreases
due to water loss caused by low temperatures.
The olive fruit is a drupe, which consists of epicarp, mesocarp (esh) and
endocarp (pit or stone). The epicarp represents 1.53.0% of fruit weight. The
greatest part of the fruit is the mesocarp which, depending on cultivar,
constitutes 7090% of the fruit and yields 90% of the oil. After pollination and
fertilization of the owers, fruit growth starts. Initially the endocarp becomes
hard, but afterwards the esh starts to grow (Rapoport et al., 1990). The fruit,
as ripening proceeds, changes colour from green to yellowish green and nally
blue or bluish black. Ethephon application promotes earlier ripening (Rugini et
al., 1982). As the fruit grows from July to November sugar content is reduced;
the remaining sugars are fermented during the processing of green olives. This
decrease is followed by an increase in the synthesis of olive oil.
From fruit setting to olive fruit maturation the timespan is 67 months,
and the fruit generally follows three growth phases in most varieties:
1. First Phase. Initially, fruit growth is rapid during the rst 2 months
(JuneJuly). During this period the weight of the fruit increases, mostly due to
endocarp growth rather than to esh (see Figs 21.1, 21.2, 21.3, 21.4 and
21.5). Polyphenols increase and later decrease (see Fig. 21.6), while
hydroxytyrosol decreases with ripening (see Fig. 21.7).
2. Second Phase. During the period AugustSeptember fruit growth is faster,
the esh grows and pit hardening occurs.
3. Third Phase. The growth of fruits is rapid (October) and the fruit starts to
change colour from green to bluish, and nally to black. The changes in fresh
weight and percentages of water, oil and dry weight are presented in Figs 21.1,
21.2, 21.3 and 21.4.
In the cv. Koroneiki ve stages of fruit growth are recorded:
1. Stage 1. This lasts from May to the middle of July. During this period the
individual fruit parts (exocarp, mesocarp and endocarp) are obvious.
2. Stage 2. During this stage (middle of July to end of August) the rate of fruit
growth is smaller compared with that in stage 1 but greater than that of the
third stage.
Olive Ripening
231
Fig. 21.1. The seasonal changes in fresh weight, water content, oil content and dry
weight of olive fruits.
Fig. 21.2. The seasonal variation in ash content of olive cv. Koroneiki during fruit
growth and ripening.
232
Chapter 21
Fig. 21.3. The seasonal changes in percentage dry weight and water content in
olive fruits.
Fig. 21.4. The seasonal changes in oil content (%) of the olive cv. Koroneiki
during fruit growth and ripening.
Olive Ripening
233
Fig. 21.5. The seasonal changes in reducing sugars (%) of the olive cv. Koroneiki
during fruit growth and ripening.
Fig. 21.6. Diagram showing the time course of olive fruit polyphenol levels and
colour changes.
234
Chapter 21
Fig. 21.7. Levels of hydroxytyrosol and its derivatives during fruit ripening as a
function of the irrigation method used during the period 2003/2004 (rainfed or
regulated decit irrigation, RDI).
3. Stage 3. The main characteristic of this stage is the small rate of fresh weight
increase, starting early in September and lasting to around 20 October.
4. Stage 4. The growth rate is rapid, commencing at the end of October and
continuing to early December.
5. Stage 5. This starts in the middle of December and ends during olive fruit
harvesting.
FACTORS AFFECTING OLIVE FRUIT SIZE AND PRECOCITY

OF RIPENING
1. Genotype. Based on fruit size, olive varieties can be distinguished by those
with small, medium or large fruits.
2. Tree age and vigour. Young trees produce larger fruits and their ripening is
more precocious.
3. Soil moisture and mineral nutrition. The soil moisture should be adequate,
especially during the rst stages of fruit growth, when cells expand. Dry winds
result in early maturation and fruit shrivelling.
4. Olive orchard orientation. Olive orchards oriented in a westerly direction
are more exposed to sunshine and their fruits ripen earlier. Furthermore, from
such orchards the oil produced has a higher concentration of aromatic
components.
Olive Ripening
235
COMPONENTS OF THE OLIVE FRUIT

During the period of fruit growth and development fruit composition varies
(Nergiz and Engez, 2000), and a small quantity of olive oil is produced while
the maximum quantity is produced during ripening. The maximum quantity
of olive oil is produced in the mesocarp (9698%), with the remainder in the
endocarp. The olive fruit esh contains oil, water, sugars, proteins, organic
acids, pectin, pigments, avonoids (Vlahov, 1992), sterols, phenols and
especially oleuropein, tocopherol and minerals.
Organic acids of olive oil

Most of the organic acids in olive oil are unsaturated. The most common acid is
oleic acid (C18:1), followed by linoleic acid (C18:2), linolenic acid (C18:3),
arachidonic acid (C20:4) and palmitic acid. Among the saturated organic
acids, palmitic acid (C16:0) and steatic acid (C18:0) are the most common. The
glycerides of oleic acid represent 7080% of olive oil and are in a liquid state
at room temperature. Oleic acid ranges between 56 and 83%, linoleic acid
3.5 20% and palmitic acid 7.520%. The percentage composition of virgin
olive oil with respect to organic acids is presented in Table 21.1. Table 21.2
shows the composition of the non-saponiable component of virgin olive oil
and the oil produced from the endocarp. Table 21.3 shows the content of virgin
olive and other oils with regard to organic acids. Ohbogge and Jaworski (1997)
give details of the regulation of fatty acid synthesis.
Water content
Water represents 70% of the fresh olive fruit weight. However, this percentage
depends on various factors such as stage of fruit growth, cultivar and water stress.
Table 21.1. Organic acid composition of virgin olive oil.
Fatty acid
Oleic acid
Palmitic acid
Linoleic acid
Steatic acid
Palmitoleic acid
Linolenic acid
Myristic acid
Arachidonic acid
Lignoceric acid
Heptadecanoic acid
Composition (%)
56.083.0
7.520.0
3.520.0
0.55.0
0.30.5
0.01.5
0.00.1
> 0.8
> 1.0
> 0.5
236
Chapter 21
Table 21.2. Percentage composition of the non-saponiable component of virgin

olive oil and of the oil produced from the fruit endocarp.
Component
Squalene and other hydrocarbons
Sterols
Sterol esters
Triterpenoid alcohols
Higher fatty alcohols
Waxes
Antioxidants, etc.
Virgin olive oil
Stone oil
3050
15
10
2545
12
25
1
10
15
2
35
Table 21.3. Comparison of organic acid content (%) of virgin olive oil with other oils.
Fatty acids
14:0
16:0
18:0
20:0
22:0
24:0
14:1
16:1
18:1
20:1
22:1
18:2
18:3
Olive
oil
Maize
oil
Linseed
oil
Soybean
oil
Cotton
seed
oil
Groundnut oil
Sunower
oil
Sesame oil
t
14
2
t
2
66
16
13
4
t
t
29
54
6
4
t
22
16
52
t
11
4
t
t
25
51
9
1
23
4
t
t
2
24
40
t
6
5
2
3
1
t
61
23
11
6
29
54
1
10
5
40
45
t, traces.
Sugars
Olive fruits contain the following sugars: glucose, fructose, mannose and
sucrose. The sugar concentration is high in the edible green olives. Sugars are
very important during fermentation in the production of lactic acid, which
gives olives their characteristic avour and also increases their storability.
Sugar levels, standing at 10% initially, are reduced to 12% during the
complete ripening process. The olive oil content, at 1% initially, is increased
gradually with the progress of ripening. The olive fruit passes through the stages
of green, red, bluish and black during ripening and ripens in 78 months from
owering. In the green stage, the fruit photosynthesizes, in the same manner as
Olive Ripening
237
the leaves, and contains a high concentration of starch and tannin. Slowly, the
olive oil is produced in the fruit and chlorophyll and starch disappear.
Oils
Fruit weight increases from September to December, almost solely due to oil
synthesis. Olive oil contains triglycerides, carbohydrates and alcohols
(0.51.5%). This process continues even when the day temperature falls below
13C. The production of triglycerides and elongation of the fatty acid chain are
a function of time. Through desaturation, oleic acid concentration increases.
Temperature decrease is the determining factor, which triggers the synthesis of
mono- or polyunsaturated fatty acids. Elongation leads to the formation of
chains of fatty acids longer than C16. Desaturation and the process of
elongation produce oleic acid, and the desaturation of oleic acid (C18:1)
produces linolenic acid (C18:2). The total yield and harvesting time both affect
oil content (Lavee and Wodner, 2004).
Oleuropein
This is the substance that gives the bitter avour to olive oil and olives and
belongs to the polyphenol group. Oleuropein levels are reduced during
ripening (Tovar et al., 2002). Oxidation of oleuropein gives the black colour to
ripe olives. Oleuropein under alkaline pH is transformed to caffeic acid. During
the processing of olives, oleuropein is removed by successive leaching with tap
water or by using NaOH and subsequent leaching.
Proteins
The olive fruit contains 1.53.0% protein. Furthermore, the olive fruit esh
contains free amino acids such as arginine, glutamic and aspartic acid.
Chlorophyll and carotenoids

The most important pigment of olive oil is chlorophyll (StefanoudakiKatzouraki and Koustsaftakis, 1992; Minguez-Mosquera and GarridoFernndez, 1989), which gives the oil its green colour. However, the presence
of chlorophyll under light conditions is the causal factor of oxidative
degradation of the oil. Both chlorophylls and are present in olive oil; the
concentration of chlorophyll in virgin olive oil can be as high as 10 ppm. When
olive fruits are harvested unripe, chlorophyll concentration is greater.
238
Chapter 21
Three chloroplast pigments identied in olives are those of all green plant
tissues and do not undergo any change or modication during the stages of
ripening. The carotenoids 3-carotene, phytouene and luteoxanthin are
present in very small amounts. Both chlorophylls and carotenoids decrease as
the season progresses, almost disappearing at the moment of maturity, while
anthocyanins begin to appear, little by little, invading the skin and later the
whole pulp. The -chlorophyll is the major component, followed by chlorophyll. Carotenoids have been found to be minor components, such as
lutein, which is the major xanthophyll, and -carotene, the principal carotene.
Carotenoids present in olive oil are represented by xanthophylls, carotenoids a,
b and and lycopene. -Carotene comprises 85% and -carotene 15% of
carotenoids. As olive fruit ripens, photosynthetic activity decreases and
chlorophyll disappears. As a consequence, the colour of the skin changes from
green to yellow, reddish or red. During this period the concentration of
carotenoids and chlorophylls diminishes, while the proportion of xanthophylls
increases. Chlorophyll degradation is accompanied by the synthesis of other
compounds, anthocyanins, because the carotenoids do not produce the nal
pigmentation of the ripe fruits, i.e. reddish or purple.
Sterols
Olive oil contains various sterols such as campesterol (12%), stigmasterol
(0.5%), -sitosterol (89.5%), avenasterol (8.0%) and traces of cholesterol. The
sterol concentration of olive oil is 180265 mg/100 g. Olive oxidation reduces
the concentration of sterols. The most common sterols found in oils of plant
origin are campesterol, stigmasterol and -sitosterol. Determination of sterol
concentration provides a means of determining the authenticity of olive oil.
Aromatic compounds
Of the aromatic substances giving olive oil its characteristic aroma are included
40 aldehydes (C7C12), monosaturated aldehydes and terpenes (see Table 21.4).
Tocopherols
These are present in all oils of plant origin, and also in animal lipids. The
-tocopherol comprises 88.5% of all tocopherols in olive oil. The concentration
of -tocopherol in olive oil is 12150 ppm. Oils derived from the fruit stone
contain higher concentrations of tocopherols, which play the role of antioxidants. The concentration of tocopherols provides a means of determining the
authenticity of olive oil (see Table 21.5).
Olive Ripening
239
Table 21.4. Some of the aromatic components of olive oil.

Hydrocarbons
Aldehydes
Aliphatic alcohols
Isopentane
2-methyl-pentane
Hexane
Octane
Napthalene
Ethyl napthalene
Acetaldehyde
Propanol
2-methyl-butanol
Butanol
Pentanol
Methanol
Ethanol
Isopropyl alcohol
1-pentanol
3-methyl-butanol
1-penten-3-ol
1-exanol
1-heptanol
1-octanol
Monoenole
2-phenylethanol
Table 21.5. Tocopherol concentrations of certain oils of plant origin.

Tocopherols (mg/g)
Oil
Olive oil
Cotton seed oil
Maize oil
Soybean oil
Groundnut oil
+
Total
0.24
0.56
0.26
0.07
0.23
t
0.38
0.92
0.78
0.31
t
t
t
0.24
t
0.25
0.94
1.18
1.09
0.54
t, traces.
Phenols
These are antioxidative substances and are present in both leaves and fruit
(Ryan et al., 1999; Gomez-Rico et al., 2006). Important phenols include tyrosol
and hydroxytyrosol, which are produced after oleuropein hydrolysis. A
signicant portion of the phenols from the fruit esh is removed with the olive
mill wastes. Figures 3.6 and 3.7 present the mean ripening periods of 25 Greek
olive cultivars and 36 cultivars cultivated on a worldwide basis.
Mineral analysis of olive fruits

The overall composition of minerals varies between olive cultivars. Of the
various elements K is the most abundant element in the fruit, followed by Mg,
Ca, Na and Fe (Nergiz and Engez, 2000).
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Chapter 21
RIPENING-RELATED CHANGES
Softening of the cell wall
The study of ripening in the olive is of great interest, because the ripening stage
of the fruit inuences the industrial production of olive oil and olive processing
(Jimenez et al., 2001). Ripening is usually characterized by softening due to
modication and/or degradation of cell wall components. Changes in cell
wall polysaccharides (Mafra et al., 2001) and cell wall-associated enzymes
(Fernndez-Bolanos et al., 1995; Mazzuca et al., 2006) play a signicant role in
determining olive fruit texture during ripening. During this process the changes
occurring are characterized by an increase in the solubilization of pectic and
hemicellulosic polysaccharides, i.e. an increase in the amount of arabinose in
pectic polysaccharides and a decrease in the degree of methylesterication of
pectic polysaccharides. With regard to cell wall phenolics, p-coumaric acid
levels increase at harvesting time (Briante et al., 2002a).
Changes in chloroplast pigments

The olive fruit during the ripening process becomes purplish black at the same
time as the oil content increases. During ripening, photosynthetic activity
decreases and both chlorophylls and carotenoids decrease progressively
(Stefanoudaki-Katzouraki and Koustsaftakis, 1992). At maturation the purple
colour of the olive fruit can be ascribed to the formation of anthocyanin. These
changes with ripening are expressed in pigment composition of the olive oil.
However, during crushing and malaxation, chlorophylls are transformed into
their Mg-free derivatives. The pigment content of olive fruits is a function of
their genotype.
Changes in catechol oxidase

Catechol oxidase is the main enzyme causing browning in fruits and other
tissues, and is located mainly in the chloroplasts (Ben-Shalow et al., 1977). The
amount of o-diphenols, which can serve as substrates for catechol oxidase,
often declines during olive fruit development. The total o-diphenol content in
the olive continues to rise parallel with fruit growth. The phenolic content can
be one of the factors determining the rate of enzymatic browning, and the
browning potential of olives will not change during development. In green
olives there is a tight binding of the enzyme to chlorophyll-containing
membranes. During the nal stage of fruit development, when the olive
blackens, the enzyme appears predominantly in the soluble fraction and these
Olive Ripening
241
changes coincide with the appearance of anthocyanins. The properties of

catechol oxidase are essentially the same in both green and black olives.
PHENOLIC AND VOLATILE COMPONENTS OF EXTRA

VIRGIN OLIVE OIL
Ripening level of olives could be evaluated by using any of the following
methods: harvesting day, ripening index (RI), fruit skin colour, fruit rmness
and amount of chlorophylls and carotenoids in the oil. Oil quality is evaluated
by using the parameters of acidity, K232, K270, peroxide index and panel test
(Yous et al., 2005). The degree of ripening of the fruit and irrigation
management signicantly affect the amounts of secoiridoid phenolic
compounds (Tovar et al., 2002; Prenzler et al., 2003). These compounds
greatly decrease upon increase of both ripening and irrigation. Furthermore,
secoiridoid derivatives of hydroxytyrosol decreased more than those of tyrosol.
Hydroxytyrosol has a protective role in the human erythrocyte against
oxidative damage (Lavee and Wodner, 1991). Oil quality evaluated using the
parameters of acidity, K232, K270, peroxide index and panel test was not affected
by fruit ripening or by increased rainfall (Yous et al., 2005).
FRUIT RIPENING AND OLIVE OIL QUALITY

Harvesting of olives at the green stage of ripening results in a reduced yield.
However, this permits easier handling of harvested olives, since green olives are
more resistant to mechanical damage and fungal infection than the more ripe
fruit. Soft olives should be processed as soon as possible, because they lose the
initial quality of their oil very rapidly (Garcia et al., 1996). The oil content is
more or less stable during the different ripening stages. Furthermore, the oils
obtained from olives at more advanced ripening stages have an increased
content of conjugated fatty acids; the bitterness index of the oil decreases as
ripening progresses. In general, the parameters that are used to measure the
oxidation of the oils (K232, K270 and stability to oxidation) show a progressive
deterioration of oil quality as the ripening process proceeds. As the olive fruit
ripens, a percentage decrease in palmitic and oleic acids is observed, while that
of linoleic acid increases.
PHENOLS IN OLIVE OIL

Oleuropein, the bitter substance of olives and olive oil, is present in the aglycone
form. As maturity proceeds oleuropein is hydrolysed, producing other
242
Chapter 21
substances which participate the characteristic taste of olive oil. Another

process taking place is oxidation of phenolic compounds and the production of
substances contributing to the aroma and avour of olive oil. The presence of
phenols gives a bitter and pungent taste, this being due to inactivation of
ptyalin and to various interactions between phenolic compounds and the
human sensors of taste.
In olive oil the bitter taste is due to phenolic compounds and cinnamic
acid, while the pungency is due to oleuropein. The amount of phenolic
compounds in olive oil cultivars varies from 150 to 700 ppm and is affected by
various factors such as cultivar, degree of maturation, climatic conditions and
pest damage to fruit. The best stage for picking is when the colour changes from
pale green to brown. At this stage the olive oil has a high phenolic content.
Furthermore, the best way of harvesting is hand-picking, and fruit processing
should be conducted at temperatures < 30C in order to maintain its high
phenolic content. Green olives give oil high in phenolic content and with a
more aromatic and fruity avour.
MINOR CONSTITUENTS OF OLIVE OIL

Among the minor constituents of virgin olive oil the most important
contributing to oil colour are - and -tocopherol (200 mg/kg) and -carotene
and chlorophyll, which give the oil its colour (yellow or green). Other minor
constituents include phytosterols, squalene, pigments, terpenic acids, avonoids
(luteolin, quercetin) and phenolic compounds known also as polyphenols.
Other minor components present are coumaric, ferulic and caffeic acids.
EFFECT OF STONE REMOVAL ON VOLATILE COMPOUNDS

OF OLIVE OIL
Olive oils produced after stone removal have a greater concentration of C6 volatile
compounds. This greater C6 concentration in de-stoned fruits is related to the
release of the membrane-bound enzymes participating in the lipoxygenase (LOX)
pathway. The reason for this is more effective pulp grinding, which causes the
cellular disruption that releases the enzymes. De-stoning increases the hexanol
content of olive oil, and this has positive effects on avour, especially the fruity
aroma.
METHODS FOR EVALUATION OF MATURATION STAGE

An acceptable method for maturity index (MI) evaluation of olives should be
objective, cheap, non-destructive and easy to apply. Various methods have been
Olive Ripening
243
used in order to achieve this, including: (i) measurement of contents of soluble

solids, starch or organic acids; (ii) measurement of respiration rate or ethylene
production; (iii) fruit size; or (iv) skin colour.
The most common method is the ripening index (RI), a subjective method
that takes into account the changes in the skin and esh colour during the
process of fruit ripening. This method is not valid for the olive varieties that
undergo a range of colour changes as maturity proceeds. Some methods are
based on the fruits resistance to compression, which is a destructive method.
Other methods, non-destructive, include the use of a hand densimeter or those
based on transmission of acoustic waves through the fruit.
A maturity index (MI) has been developed to help producers categorize
their fruits maturity level. The MI will help determine when each variety
should be harvested in order to achieve the desired product quality. For MI
calculation a sample of 100 olive fruits is collected and separated into eight
colour categories, as follows (UC Cooperative Extension, 2006):
0, fruit surface is deep green and the fruit is rm.

1, fruit surface is yellowish green and fruit starting to soften.
2, < 50% of the fruit surface is red.
3, > 50% of the fruit surface is red.
4, 100% of the fruit surface is purple.
5, 100% of the fruit surface is purple or black; 50% of the esh is purple.
6, 100% of the fruit surface is purple or black; > 50% of the esh is purple.
7, 100% of the fruit surface is purple or black; 100% of the esh is purple.
For MI calculation we multiply the number of fruits of each colour

category by the number with that colour category (07):
MI =
A N1 + B N2 + C N3 + D N4 + E N5 + F N6 + G N7
100
CHARACTERISTICS OF TABLE OLIVES AND CRITERIA

FOR RIPENING
Table olives should have the following characteristics:
High esh:pit ratio, ranging from 5 to 12.

High sugar concentration, 56%.
Relatively small oil content around 1220% of the fresh weight of the
pulp. A greater oil content is disastrous for the storage of table olives, since
product taste deteriorates and the fruit softens.
The esh should be rm. Hence, the fruit can maintain its good characteristics during both the various stages of processing and the subsequent
storage of the processed olives.
244
Chapter 21
Easy removal of the pit from the esh.

Fruit epidermis should be thin, elastic and tolerant during processing.
The criteria for ripening are the following:
Olives destined for oil production are harvested when the fruit is ripe. This
means that a period of 78 months should elapsed after owering.
Table olives are harvested when fruit growth is complete and the colour
starts to change, for Californian-type olives. For green olive processing
harvesting starts earlier, before fruit softening commences. Fruit size is a
good criterion for harvesting for some table olive cultivars.
22
OLIVE FRUIT HARVESTING
INTRODUCTION
This chapter outlines the various methods employed in the harvesting of olive
fruits, from the simple and traditional to the modern and sophisticated.
HAND-HARVESTING
Most olive harvesting around the world still involves the traditional methods,
i.e. picking by hand (Martin et al., 1994b). Labourers pick the fruits one by one
or beat the tree, with poles, causing fruit drop. Under the trees, nets or canvases
are placed to collect the fallen fruits. This method of harvesting is timeconsuming, involves a high input of labour and a high percentage of fruit
damage. Also, the olive fruit may simply drop through natural forces on to the
harvesting nets. The cost of labour at harvesting represents 5580% of the
total labour cost of olive culture.
The use of poles for olive harvesting increases the tendency of trees to
biennial bearing, because it damages a signicant number of annual shoots
necessary for the following years crop.
Harvesting of olive fruit by hand, while the fruit is attached to the tree,
gives a high-quality fruit, while harvesting of olives allowed to drop naturally
to the ground produces olive oil with a high acidity and unpleasant taste.
HAND-HARVESTING WITH ABSCISSION CHEMICALS

In more recent times growers have started to promote abscission by the use of
chemicals such as Ethrel or Alsol, which reduce fruit detachment force (FDF).
The classical harvesting method for olive oil varieties is by beating the shoots
and collecting the fruits one by one from the soil surface, but this method incurs
a high labour cost. Furthermore, labourers are not available during the period of
245
246
Chapter 22
unfavourable climatic conditions (low temperature and precipitation). Today,

new methods involving tree-beating and harvesting machinery are used.
However, their suitability depends on the cultivar (Al-Jalil et al., 1999).
PNEUMATIC BEATING POLES

These poles should have minimum weight, and therefore they are constructed
from plastic or aluminum material. This method is suitable for the harvesting
of low-fruited and shorter varieties. Tools previously used had a low harvesting
cost, short harvesting time and did not damage the tree. Pneumatic beating
poles are used when the olive fruit is ripe, since the FDF is reduced; they are
recommended only for varieties destined for olive oil production.
The best beating poles revolve, since they have a greater efciency and do
not damage the trees. In the commercial market two types are available, i.e. the
linear and the T-shaped (see Fig. 22.1). Each pneumatic beating pole has a
vertical axis revolving with the aid of a motor. At various locations on the
vertical axis are attached easy-to-bend accessories, four to six at each level. The
fruits drop on to plastic nets or canvases placed on the soil surface below the
tree canopy. Workers should use the pneumatic beating pole in a rapid fashion,
since beating the same shoot for a relatively long time damages the shoots and
causes their defoliation.
The linear beating pole is recommended for most varieties, with the
exception of Koroneiki. This machinery is highly efcient one operative
(b)
(a)
(c)
Fig. 22.1. Three versions of olive-beating machines: (a) with horizontal branches;
(b) with T-shape; and (c) with a vertical axis.
Olive Fruit Harvesting
247
can harvest around 1000 kg/day. The T-shaped beating pole can harvest
1500 kg/day and is appropriate for all varieties. This version incorporates
six to eight exible beating poles, which revolve and harvest the fruit. The
percentage leaf drop with this device is lower in comparison with the linear
beating pole. The pneumatic beating pole is powered from a 12 v battery or
from an air compressor of 68 atm pressure, adapted to a tractor.
All types of machinery above should be tolerant to stress, have a high
degree of efciency and not be too heavy in use. After completion of the beating
of shoots, the olive fruits are harvested from plastic nets or canvases, or the
collection may done by hand if no nets are used. The soil should be at and
without stones; otherwise, the soil should be levelled off. Harvesting from the
ground is accomplished by a rotating cylinder with needles, which collect the
fruits. This method, however, damages the fruits and results in deterioration of
oil quality.
HARVESTING NETS AND CANVASES

The use of plastic nets and canvases is very common in Mediterranean
countries as an aid in olive fruit harvesting, in conjunction with the use of
pneumatic beating poles. Nets and canvases should be tolerant to mechanical
and light damage and not heavy (around 100 g/m2). After harvesting, nets
and canvases are cleaned and dried under shade and stored in warehouses for
the next harvesting period. The most common colours of nets available are
white, black and green, and their sizes vary from 3.3 5.0 to 6.5 12.0 m.
The nets are manufactured from polyethylene, improved to resist UV radiation.
In certain heavily sloping olive groves with large trees that are not regularly
pruned the nets are placed in their position permanently and the olives simply
drop onto the nets. From time to time the fruits are collected from the nets.
With this method the fruits may be damaged by insects and diseases, while the
oil loses its aromatic components and ne taste.
After harvesting the fruits are carried in plastic bags or containers, each
containing 210420 kg, to the oil-processing unit. These containers are
manufactured from high-quality plastic, not wood, for better aseptic conditions.
HARVESTING AND GATHERING TOOLS

Scissors-type harvester
This tool consists of two combs with prolongations made of hard plastic
material. When the harvester is closed and pulled, it harvests the fruits from
small twigs without removing the leaves.
248
Chapter 22
Tweezers-type harvester
The main parts of this tool comprise two rotating cylinders. When the device is
closed the small shoots pass through the two cylinders and the fruits are
detached. Afterwards, collection of the detached fruits from the ground takes
place.
Manually operated cylinder harvester

This tool has one cylinder with plastic needles that rotate; fruits dropped on the
ground are harvested, then collected in a metal basket.
Plastic nets
Plastic nets are placed below each tree canopy before harvesting. These have
the disadvantage of their signicant cost and short duration of life. In order to
avoid olive drop on the ground, the nets are placed under the trees early and
remain there until the end of the harvesting period. Therefore, around 32% of
the olives are collected in the nets by natural drop and the remaining portion
after shoot-beating with pneumatic poles. Beating of olive trees increases the
tendency for biennial bearing. When using mechanical vibrating harvesters,
certain factors such as fruit tolerance to abscission, fruit weight, tree shape and
the power of the harvester may affect harvesting success. For reduction in FDF,
various substances have been tried, with varied results.
MECHANICAL HARVESTERS
The conventional methods of olive growing and the popularity of tall trees both
increase the cost of harvesting. Recent decades have seen the introduction of
harvesting for olives and other fruits by mechanical harvesters, which can
vibrate either only one branch or the whole tree The efciency of vibration and
its transmission is affected by the cultivar (Antognozzi et al., 1990a, b; Visco et
al., 2004). Each mechanical harvester consists of the vibration system, the
collection system (umbrella) and the fruit suction system (see Fig. 22.2). The
method of fruit collection consists of two semicircular pieces of cloth attached
to a metal skeleton, placed under the olive tree canopy.
This is an innovative type of harvester, incorporating an excavator that
controls the shaker and the gathering umbrella. It can harvest 15 or more olive
trees/h and has a harvesting efciency of 90.5%. The force necessary to vibrate
one branch is 810 Hp, and the trunk 40120 Hp; the maximum vibration
249
Fig. 22.2. Mechanical trunk vibrator with a reverse umbrella harvesting an olive
tree (from Tombesi, 2006).
power is 4000 cycles/min, suitable for the trunk 1200 cycles/min are
recommended for branches (see Fig. 22.3). Only single-trunked trees are
suitable for harvesting by this device. The fruit suction component consists of a
rotating axis, which creates the suction force. Furthermore, the air current
produced removes the leaves and other foreign material from the harvested
olives.
Over-row harvester
This harvester is derived from grape or coffee harvesters and vibrates the olive
fruit branches by means of ten to 12 shaking bars (see Fig. 22.4). The fruits are
collected by a chain, with baskets running at the same speed as the machine.
Fruit removal percentage is high (9095%), even in small-fruited varieties.
The harvester moves with a speed of operation between 0.3 and 1.0 Km/h. An
alternative system consists of a vertical axis with lateral sticks that vibrate.
Trees suitable for harvesting by this type of harvester should be lower than
2.5 m and no wider than 1.5 m, with elastic branches; therefore, dwarf and
very productive varieties are most suitable. For the SHD orchard (1600
trees/ha) Arbequina and Arbosana are the best varieties. Arbequina showed
the highest total production between the third and sixth years following
planting, followed by Arbosana and Koroneiki. Arbequina has a semi-erect
habit, Arbosana an open habit. In SHD orchards the objective is to attain
250
Chapter 22
Fig. 22.3. Trunk shaker for olive harvesting. The fruits drop and are collected on a
net or canvas placed under the tree canopy.
Fig. 22.4. An over-row olive harvester harvesting a super-high-density olive grove

(from Tous et al., 2006).
production by the third or fourth year, to use mechanical pruning and to use
efcient harvesting machines, in order to reduce costs. However, it is necessary
to determine for how many seasons the plantation remains economically
productive.
251
FEATURES AND ADVANTAGES OF MECHANICAL

HARVESTING
There are many reports in the literature dealing with mechanical harvesting of
olives and its limitations and advantages (Mannino and Pannelli, 1990;
Martin, 1994b; Tombesi et al., 1998; Giametta, 2001; Ravetti, 2004).
Harvesting cost is one-third to one-half of that of hand-harvesting.

The speed of harvesting is greater; therefore, pests and diseases are avoided.
The olive fruits are not in contact with the soil surface. Therefore, they
avoid infection by microorganisms.
Harvesting requires very few operatives. One mechanical harvester can
harvest ten to 15 trees/h or more with only two or three operatives.
Mechanical harvesting demands groves of limited slope and adequate

planting distances, in order to permit the movement of machinery. Furthermore,
the tree canopy should be trained appropriately. Trees intended for harvesting by
a trunk vibrator have the following characteristics:
The trees should have only one trunk, 1525 cm or more in diameter and
trunk height 1 m in order to allow easy attachment of the vibrator to
the trunk.
The shoots located at the lowest part of the tree canopy should be cut, since
they represent obstacles to the use of a mechanical harvester.
The trees should have no more than three branches; if not, these are
removed. Experience has shown that branches of mature trees < 15 cm in
diameter are not suitable for vibration.
In the case of branch vibrators the main branches should be arranged in

such a way that the harvester can vibrate them without the need to change
position. This reduces the harvesting time required for each tree, and therefore
harvesting cost.
The proportion of fruits harvested by the mechanical harvester depends on
the following criteria.
Vibration characteristics
The greater the acceleration the more easily the fruits are harvested. The
acceleration produced by a mechanical harvester is compared with the natural
forces required by the fruit in order to drop, and this is described by the
following equation:
Fruit acceleration = Fruit weight/Required force for fruit removal.
252
Chapter 22
Force of fruit detachment

This force is measured by dynamometer, and decreases signicantly after
spraying the fruits with one of the following chemical loosening agents (Martin
et al., 1981; Ben-Tal and Wodner, 1997; Metzidakis, 1999):
Cycloheximide.
Ethrel (ethephon, 2-chloroethyl-phosphonic acid), producing ethylene
(Hartmann et al., 1970; Ben-Tal and Lavee, 1976b; Ben-Tal et al., 1979;
Lavee and Martin, 1981).
CGA (2-chloroethyl-tris-2-methoxy-ethoxy silane, or Alsol).
Chloroethyl-sulphonic acid.
These substances are sprayed 57 days before the date of mechanical

harvesting. The use of ethephon in order to enhance fruit ripening is very
common. Ethephon is used commercially in order to facilitate mechanical
harvesting or hand-harvesting of olive fruits (Ben-Tal and Lavee, 1976a; BenTal, 1987, 1992; Denney and Martin, 1994; Gerasopoulos et al., 1999).
Environmental factors affect ethephon efciency in the olive (Klein et al.,
1978). From the applied ethephon only an amount lower than 3% penetrates
the olive pedicels and releases ethylene to promote abscission (Martin et al.,
1994b). Ethephon is not translocated from leaves and fruits to the fruit
pedicels.
When we use looseners we attempt to achieve maximum fruit removal
with minimum leaf abscission. Ethephon application induces the onset of leaf
abscission 3660 h after treatment and, in the following 812 h, separation is
evident (Martin et al., 1994b) in the abaxial cortical cells close to the vascular
system. Separation in the adaxial side then proceeds. Finally, separation takes
place in the vascular region and the epidermal cells. During the abscission
process, starch is accumulated near the vascular region.
The abscission of olive fruit occurs in two abscission zones, one between
the peduncle and pedicel and the other between the pedicel and fruit.
Application of ethylene-releasing compounds results in abscission mostly in
the pedicel/fruit zone. However, fruit abscission may be observed in the
peduncle/pedicel zone. Under eld conditions, ethylene action is sometimes
variable due to variations in temperature and moisture conditions. High
relative humidity (RH) reduces the speed of ethylene decomposition, while low
(3570%) RH speeds up ethylene decomposition, especially when the
temperature is high. Furthermore, both the duration and concentration of
ethylene-releasing compounds affect fruit abscission. An optimum fruit
abscission is achieved by applying about 3 ppm ethylene for 28 h. In olive fruits,
ethylene production is low, i.e. there is no ethylene autocatalytic enhancement.
However, olive leaves show autocatalytic enhancement 120 h after external
application (Martin et al., 1994b).
253
Date of harvesting
As maturation proceeds the FDF is reduced and the fruits are harvested more
easily. The ratio of FDF:fruit weight is reduced with the progress of ripening, but
starts to increase again after the middle of the growth period.
Olive varieties with small fruits have a high F:W ratio (F, force, W, weight).
In contrast, large-fruited varieties with high FDF have a low ratio. The F:W
ratio may constitute a criterion for ease of fruit removal (Tsatsarelis, 1987). In
olives the maximum fruit abscission with a low leaf drop, by use of ethylenereleasing chemicals, is of great signicance. Once ethephon is applied, fruit
abscission starts within 3660 h; even after 810 h separation in the
abscission zone is obvious. Histochemical examination of the fruit abscission
zone indicates that considerable starch is accumulated close to the vascular
bundles, and its content does not change during abscission.
Efciency of mechanical harvesting

Shaking tests have shown that the percentage harvesting of olives without
application of a loosener was < 50%; application of abscission chemicals
reduced the FDF:W ratio and thus increased this gure. The highest fruit
removal (96%) was obtained by using a concentration of 12.5 ml/l of
ethephon. The frequency used for the individual fruitstem system is a key
criterion in the design of a mechanical harvester that uses vibration. The
detachment of fruits is mainly a function of the applied frequency, the total time
of shaking and the relative proportions of mature and immature fruits on the
tree (Sessiz and zcan, 2006).
Variety in tree size and agronomic parameters affect harvesting efciency
(Dias et al., 1999; Proietti et al., 2002b). Of the training systems in use, the vase
form showed a slightly greater adaptability to mechanical harvesting as
compared with the monocone form. This is most probably due to the higher
deviations and distance from the main branch of fruiting branches in the
monocone system. The oil obtained during the early periods of harvest had a
higher polyphenol content and was more fruity, bitter and spicy than that
obtained from the later harvesting periods.
Factors affecting efciency of trunk shakers

The most important factors affecting the efciency of mechanical trunk shakers
are:
1. Planting distance: planting distances of 6 6 or 6 7 m are ideal for the
reverse umbrella technique.
254
Chapter 22
2. Training system: olives should be trained in such a way that there is only
one trunk, 1.01.2 m in height.
3. Cultivar: the variety affects the shaking efciency, since that determines
fruit size, detachment force, maturity pattern and branch elasticity.
4. Fruit size and its FDF: fruits > 2 g are harvested easier than small fruits.
Fruit size also determines the fruit detachment force the FDF should be
around 4N (Newton), in order to facilitate the efciency of the harvester.
5. The optimum canopy volume is 4050 m3 (Tombesi, 2006).
MOLECULAR STRATEGIES FOR IMPROVING OLIVE

HARVESTING
The cost of hand-harvesting olives represents more than 50% of the total
production cost. Mechanical harvesting, therefore, has many advantages
in comparison with traditional hand-harvesting. Furthermore, mechanical
harvesting efciency is increased by ethylene-releasing chemicals, which
induce olive fruit abscission. However, mechanical harvesting cause 1520%
leaf abscission and affects the following years yield.
Olive fruit is not a climacteric one and produces negligible amounts
of ethylene during maturation; such fruits produce very little ethylene.
Production of ethylene can be increased by exogenous application of ACC. This
means that the lack of signicant ethylene production by olive fruit is likely to
be ascribed to low levels of ACC synthesis in the tissue. Therefore, ethylene
production in olives can be achieved by using transgenic plants or after
insertion of an active ACC synthase into the trees. Today, transformation
methods can be used and such a strategy could be utilized to solve the problem
of the high labour cost of hand-harvesting by producing a transgenic olive
plant with ACC synthase expressed specically in the fruit at the correct
maturation stage (Ferrante et al., 2004).
23
OLIVE VARIETIES
INTRODUCTION
The olive tree has been cultivated for thousands of years and includes many
varieties, creating problems in their classication (Barranco et al., 2000). The
problem becomes more acute because, very often, the same cultivars at various
locations are known under different names (Therios, 2005b). The criteria for
classication of olive cultivars are the following:
Tree height.
Leaf and inorescence characteristics.
Fruit characteristics (shape, colour, size, percentages of esh and pit,
esh:pit ratio, shape of pit and seed, percentage of oil).
Precociousness and productivity.
Resistance to pests and diseases.
Adaptability to soil and climatic conditions.
Use of DNA markers to separate cultivars.
THE USE OF MOLECULAR MARKERS TO CHARACTERIZE

AND CLASSIFY OLIVE (OLEA EUROPAEA) GERMPLASM
The considerable diversity within olive and the presence of a great number of
synonyms require the development of efcient and rapid methods to determine
cultivars (Pontikis et al., 1980; Belaj et al., 2001, 2004). Although molecular
analysis is able to characterize olive cultivars it is not a substitute for
morphological description: it is only a complementary tool to study olive
germplasm.
Among the molecular techniques applied for cultivar identication there
are four major ones:
255
256
Chapter 23
RFLP (Restriction Fragment Length Polymorphism).

RAPD (Random Amplied Polymorphic DNA).
AFLP (Amplied Fragment Length Polymorphism).
SSR (Simple Sequence Repeats) (Martin-Lopes et al., 2007).
Other markers used, but to a lesser extent, to study genetic diversity in

olives include SCAR (Sequence Characterized Amplied Region) and ISSR
(Inter-Simple Sequence Repeats) (Martin-Lopes et al., 2007).
The RFLP markers are the molecular technique that was rst applied to
olives, the aim being to discriminate between wild and cultivated olive
varieties. The RAPD markers are used for genetic characterization in olives
(Bogani et al., 1994; Fabbri et al., 1995; Belaj et al., 2001; Besnard et al., 2001;
Hagidimitriou et al., 2005; Martin-Lopes et al., 2007). AFLP is used to
study the genetic relationship between cultivated olives, wild olives and
related species. AFLP also tests for genetic interrelationships between olive
cultivars (Bandelj et al., 2004; Hagidimitriou et al., 2005; Montemurro et al.,
2005).
SSR markers are used to study variability in olives and are also used for
variety identication. Other markers used to study olive germplasm variability
utilize chloroplast DNA variations by comparing (i) RFLP and PCR polymorphisms (Besnard and Bervill,2002; Intrieri et al., 2007); (ii) microsatellite
markers (Bandelj et al., 2002; Diaz et al., 2006a); (iii) polymorphic and
single nucleotide polymorphism markers (Reale et al., 2006); (iv) fractals
and moments to describe olive cultivars (Bari et al., 2003); and (v)
genetic distances to study the history of olive cultivars (Loukas and Krimbas,
1983).
The number of cultivars grown and their synonyms, their separation into
type (olive oil, table olives and dual-purpose cultivars) and their distribution
throughout various countries are given in Table 23.1. The main olive cultivars
grown in various countries are presented in Table 23.2, and in Table 23.3 the
taxonomy of 24 Greek cultivars is presented.
SMALL-FRUITED OLIVE CULTIVARS

Ladolia (Olea europaea var. mastoides or microphylla)
It is considered that this cultivar belongs to the species O. mastoides or O.
microphylla and is known also under the names Patrini, Kurelia or
Kutsurelia. The growth habit is erect and tree height at maturity is 57 m.
The leaves are small and the leaf blade is broader at the middle and the top. The
length of the blade is 3.35.3 cm and the length:width ratio is 3.66:1. The fruit
is spherical in shape, with a characteristic mastoid protuberance. The fruit
ripens late, from November onwards, and its weight is 1.11.62 g. The ratio of
Olive Varieties
257
Table 23.1. Number of local cultivars and synonyms by type (olive oil, table olives
or dual-purpose) grown throughout various countries worldwide.
Country
Albania
Algeria
Argentina
Australia
Brazil
Chile
China
Cyprus
Egypt
France
Greece
USA
India
Iraq
Israel
Italy
Jordan
Lebanon
Libya
Morocco
Mexico
Pakistan
Peru
Portugal
Spain
Tunisia
Turkey
Former
Yugoslavia
Total
Cultivars
Synonyms
Olive oil
Table
olives
21
51
6
2
1
9
1
6
99
60
3
10
46
476
3
13
6
6
1
1
1
19
196
50
34
28
33
2
435
67
2
13
1599
17
13
8
132
513
42
46
14
37
5
1
9
1
1
73
46
3
8
13
390
1
4
5
2
6
135
27
7
1
1
1
1
5
7
3
17
13
1
1
1
20
25
4
18
Dualpurpose
6
13
19
11
2
16
73
1
8
1
1
11
36
19
9
47
70
20
18
1188
512
812
117
259
esh:pit is 3.585.00:1 and the oil content of the fruit is 1622%. It is

considered as a good cultivar for olive oil production.
Koroneiki (Olea europaea var. microcarpa alba)

Other names of this cultivar are Psilolia, Lianolia or Korani. It is widespread
in the main olive-producing districts of Greece (i.e. Crete, Peloponnese, etc.)
and is expanding in other areas of the world too. In recent years this cultivar
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Chapter 23
Table 23.2. The main olive cultivars grown in various countries.

Country
Cultivars
Spain
Italy
Portugal
Tunisia
Algeria
Morocco
France
Turkey
Argentina
Israel
Lebanon
USA
South Africa
Greece
Picual, Verdal, Arbequina, Manzanillo, Sevillana

Frantoio, Moraiolo, Leccino, Coratina, Ascolano
Galega, Verdial, Radondil, Gordal
Chemlali
Sigoise, Azeradj, Harma
Picholine marocaine
Picholine, Anglandaou, Salonenque, Tanche
Milas, Kilis, Cakir, Ismir
Arbequina, Leccino, Frantoio, Manzanillo
Souri, Malissi, Barnea
Chami, Souri
Mission, Manzanillo, Sevillana, Ascolano
Sigoise, Barouni, Meski
Koroneiki, Megaritiki, Kalamon, Amphissis,
Kolovi, Adramytini, Lianolia, Chondrolia Chalkidikis
Table 23.3. Twenty-four Greek olive cultivars of Olea europaea and their taxonomy.
Cultivar
Taxonomy
Konservolia
Karydolia
Gaidourolia
Amygdalolia
Kalamon
Adramitini
Kothreiki
Karolia
Kolymbada
Tragolia
Mastoidis
Throumbolia
Valanolia
Megaritiki
Lianolia Kerkiras
Maurolia
Koroneiki
Vasilikada
Aguromanacolia
Kalokaerida
Myrtolia
Dafnolia
Asprolia
Chrysolia
O. europaea var. rotunda

O. europaea var. med. maxima
O. europaea var. major macrocarpa
O. europaea var. amygdaliformis
O. europaea var. ceraticarpa calamata
O. europaea var. media subrotunda
O. europaea var. minor rotunda
O. europaea var. oblonga
O. europaea var. uberina
O. europaea var. minor oblonga
O. europaea var. mamilaris
O. europaea var. media oblonga
O. europaea var. pyriformis
O. europaea var. argentata
O. europaea var. craniomorpha
O. europaea var. nigra microcarpa
O. europaea var. microcarpa alba
O. europaea var. regalis
O. europaea var. ovalis
O. europaea var. precox
O. europaea var. microcarpa subrotunda
O. europaea var. clavata
O. europaea var. alba
O. europaea var. chrysophylla
Olive Varieties
259
has started to be grown in the form of super-dense plantings all over the world
(e.g. Australia, Italy, Spain). The leaves are thick, with a small leaf blade. The
length of the blade is 4.55.2 cm and the ratio of length:width is 4.25.5:1.
The fruit is very small (0.5 g), with a mastoid shape and ending in a teat. The
fruit ripens from mid- to late season and turns black at full ripening. The pit is
small and cylindroconical in shape. The ratio of esh:pit is 1.634.06:1. The
medium yield per tree is 5060 kg. This cultivar is resistant to water stress and
wind, but sensitive to Dacus oleae, Euphyllura olivina, Pseudomonas savastanoi
and attacks from rhynchites. The olive oil content is 27%. Its tolerance to cold
is low and its rooting ability from leafy cuttings is constant and medium.
Lianolia Kerkiras (Olea europaea var. craniomorpha)

Other names of this cultivar include Suvlolia, Nerolia, Striftolia and
Prevezana. This is the main cultivar in Kerkira, a Greek island in the Ionian
Sea. The tree is very tall, 1214 m, with a maximum of 2025 m. The tree
trunk is crooked, but the trees are erect and the leaves are big, with a deep
green colour on their upper surface. The ratio of length:width is 5:1. The
average number of owers/inorescence is 2330. The fruit is small, with a
cylindroconical shape and ends in a teat. The fruits are formed in clumps of two
to six. The pit is relatively large in comparison with fruit size, and of
cylindroconical shape. The number of fruits/kg is 580 and the ratio of esh:pit
is 6.21:1. The oil content is 2022% and of excellent quality. The tree starts to
produce late, and biennial bearing is common due to late harvesting
(sometimes in spring). This is an oil-producing cultivar with high oil content. It
is partially self-fertile, with stable and high productivity but with a low rooting
potential from leafy cuttings. Its tolerance to drought, wind and cold is high,
while the tolerance to Bactrocera oleae, Cycloconium oleaginum and Armillaria
mellea is low. This cultivar is cultivated in the Ionian Sea on the Greek islands of
Kerkira, Kefalonia, Zakinthos and Paxoi.
Tsunati (Olea europaea var. mamilaris subvar. minima)

Other names of this cultivar are Muratolia and Athinolia. It is cultivated on
the Greek island of Crete. The fruit is oval in shape, with a characteristic teat
and it looks like a lemon in its shape. The fruits are borne two to three together,
ripening during the period NovemberDecember and their average weight is
1.3 g. The esh:pit ratio is 4.86:1.
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Chapter 23
Lianolia Patron
Other names of this cultivar are Ladolia, Lianolia, Kothrelia, Kutrelia,
Kutsurelia or Kutsuliera. This variety is cultivated in the Greek area of
Peloponnese. The tree height is 57 m, the leaves are small and the fruit is
spherical. The mean weight of fruits is 0.91.7 g (6001100 fruits/kg). Its oil
content is 1528%.
Mastoidis (O. europaea var. mamilaris)

Other names of this cultivar are Mastolia, Athinolia and Pitsadeiki. It is
cultivated in Crete and Peloponnese. The leaves are 67 cm in length and
1.1 cm wide; their colour is light green to green. The fruit is cylindroconical in
shape, weighing 1.42.2 g. Its oil content is 2835% and the esh:pit ratio is
6.0:1. It tolerates low temperatures and can be grown in areas up to 1000 m in
elevation.
MEDIUM-FRUITED OLIVE CULTIVARS

Megaritiki (Olea europaea var. argentata)
Other names for this cultivar are Ladolia, Perachoritiki and Vododitiki. This
is a Greek cultivar cultivated in the area of Megara and part of Peloponnese.
The length:width ratio of leaves is 6.4:1. The average fruit weight is 3.54.5 g.
The fruit has a characteristic heteromorphism in its shape. The fruits are borne
singly or in pairs and have characteristic grey spots on their epidermis during
ripening. The ratio of esh:pit in the fruit is 8.610.1:1. The oil content is
2225%. This cultivar requires pollination since it is partially non-self-fruiting
with a tendency towards alternate bearing and medium rooting ability of its
cuttings. It has low tolerance to Aspidiotus hederae, Parlatoria oleae and Saissetia
oleae, medium tolerance to cold, salinity and Verticillium dahliae and high
tolerance to drought.
Galatsaniki
Other names of this cultivar are Agiou Orous or Galatistas. This is a mediumsized cultivar with cylindroconical fruit, ending in a teat. Its oil content is 20%.
It ripens early (September) and its fruit at ripening becomes completely black.
This cultivar is sensitive to Dacus and Cycloconium, but tolerant to cold.
Olive Varieties
261
Thasitiki
Other names of this cultivar are Thasou, Throumba or Throumbolia. It is a
Greek cultivar grown on the Greek island of Thasos (northern Greece). Its fruit
is cylindroconical in shape, ending in a teat. This is a dual-purpose cultivar
with 20% oil content. The leaves are dark green and the shoots are hanging. It
is resistant to cold, Cycloconium and Bacterium tumefaciens.
Kothreiki (Olea europaea var. minor rotunda)

This is also known under the names Manaki, Manakolia, Glykomanako or
Korinthiaki. It is cultivated in central Greece and Peloponnese. The leaves are
broad, with a length:width ratio of 4.0 :1 and the fruit is spherical, without a
teat. The mean fruit weight is 3 g and the pit is cylindroconical. The ratio of
esh:pit is 4.70:1. This cultivar is resistant to cold and can be grown at up to
800 m elevation. It is a very productive dual-purpose olive cultivar.
Kalamon (Olea europaea var. ceraticarpa calamata)

This is known also by the names Kalamatiani, Aetonichi, Tsigeli and
Karakolia. It is an excellent cultivar for both table olives and oil production, and
has now begun to be expanded from Greece to other countries (see Fig. 23.1). Its
leaves are distinctively large, slightly twisted from end to end and their
length:width ratio is 4.11:1. The fruit has a mean weight of 2.65.5 g and it is
lengthy and pointed, with a distinctly bent point at its tip. The fruits are presented
singly or in pairs. The fruit starts to ripen in November and it turns black when
Fig. 23.1. The world-famous Greek olive cv. Kalamon.
262
Chapter 23
fully ripe, and it has a mean oil content of 17%. The esh:pit ratio is 6.72:1. This
is partially non-self-fruiting and its best pollinizers are the cultivars Manzanillo
or Gordales. The tree obtains a height of 710 m; its growth is erect, with broad
dark green leaves (89 cm 1.52.0 cm). The fruit has a cylindroconical shape
and the ratio of esh:pit is 8.3:1. The esh of the fruit is compact and the fruit
ripens in late NovemberDecember. It is resistant to Dacus and needs mediumtextured soils with an optimum pH of 7.0. During the summer it requires at least
three irrigations in order to produce fruit of adequate size. Since it is a vigourous
cultivar N fertilization should be carefully adjusted. This cultivar does not root
easily with leafy cuttings under mist propagation, and it is the best known Greek
olive cultivar, with a worldwide reputation. It is an excellent table variety
producing a high-quality product as regards colour, texture and taste. Although
it originated in the region of Kalamata in south-east Peloponnese, it has proved its
adaptability as an excellent cultivar in both warm and cold areas worldwide.
Kolovi (Olea europaea var. pyriformis)

This is known also by the names Valana, Milolia or Mytilinia. It is cultivated
mostly on the Greek island of Lesbos (70%), and less so on other Greek islands.
The tree is vigourous and its height is 68 m. The leaves have a large dark
green blade and their length:width ratio is 4.33:1. The fruit (36 g) has the
shape of an acorn and matures late (end of December). One to three fruits are
presented together. The pit has six to seven carvings. The oil content of this
cultivar is 25% and is of excellent quality. This cultivar is used mostly for olive
oil production and only partly for pickling of green and black table olives. It is
sensitive to Cycloconium.
Adramitini (Olea europaea var. med. subvar. otunda)

Known also under the names Aivaliotiki and Fragolia, it represents 20% of
olive culture on the Greek island of Lesbos. The leaves are of average size, with
a length:width ratio of 4.16:1. The fruit is round to slightly oval in shape. The
mean fruit weight is 2.17 g, the ratio of esh:pit is 3.82:1 and the month of
ripening is December. The oil content of this cultivar is 22% and the oil quality
is excellent. However, this cultivar is sensitive to Dacus.
Frantoio
Other names for this cultivar include Paragon (in Australia), Frantoijano,
Corregiolo, Razzo and Gentile. These ve are all considered to belong to
Olive Varieties
263
the same family or population. The cv. Paragon of Australia, after DNA
analysis, has been shown to be similar to the cv. Frantoio grown in the
Tuscany region of Italy. This is the most productive cultivar in central Italy, is
very adaptable to various conditions and is very cold resistant. The oil quality
and avour are both excellent. The productivity is light to medium and this is
one of the most important olive trees in New Zealand. It does not require
chilling for owering bud differentiation. Although this cultivar is considered
self-fruiting, the use of the cv. Pendulina as pollinator may increase the yield
by up to 10%. The proportion of pollinators should be 510% of the trees
in an orchard. Frantoio is considered as being sensitive to peacock spot
(Cycloconium oleaginum).
FS-17
This cultivar originated from free pollination of the cultivar Frantoio and it is
a widespread variety throughout Italy. The cultivar FS-17 is highly suited to
dense planting and mechanical harvesting (Fontanazza et al., 1998).
Nowadays this cultivar is grown in many parts of the world Italy, Spain,
South Africa, Argentina, Chile, Greece and Australia. It is an excellent variety
for oil production and has low vigour, the tree having a drooping appearance.
The leaves are medium-sized and elliptical. The fruit weight is 24 g and the
esh:pit ratio 9.73:1, with an oval pit. FS-17 roots very easily under mist
propagation and is self-fruiting. The owering period and ripening season are
both average. The tree starts producing early and its productivity is stable and
high. It can be used as rootstock for the cultivar Giaraffa, reducing the vigour
of the latter by 50%.
Manzanillo
This is also known as Manzanilla, Manzanilla de Sevilla or Fina. The tree is
about 7 m in height at maturity. The canopy is spreading, is highly productive
and early bearing. The tree grows better in warm locations without heavy
frosts, and it is considered to be the worlds most popular table olive, especially
in California and Spain.
The size of the fruit is medium (4.8 g, 200280 fruits/kg) and the
esh:stone ratio is 8.2:1 (see Fig. 23.2). The shape of the fruit is oval, with a
thick skin and good texture. The fruit at maturation has a slightly violetblack
colour. It is a cultivar sensitive to extended cold periods, and represents 60 and
80% of green pickling fruit for Mediterranean countries and California,
respectively. It is considered as the best dual-purpose olive cultivar in the world.
Manzanillo is susceptible to Verticillium wilt. For good production and crosspollination the cvs Sevillana and Santo Agostino are used as pollinators.
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Chapter 23
Fig. 23.2. The olive cv. Manzanillo.
The tree is very productive. It produces every year and its propagation by
leafy cuttings under mist is difcult. The fruit contains 20% oil and has a small
pit. The esh of this cultivar contains also 5.65% total sugars (5.41%
reducing). Examination by RAPD (Random Amplied Polymorphic DNA)
revealed that Manzanillo consists of 14 genotypes, as shown in Table 23.4.
Meski
Meski is a table olive cultivar that is self-sterile, with medium rooting ability
and low oil content. It has low tolerance to cold, Cycloconium oleaginum,
drought, iron chlorosis and lime, medium tolerance to Pseudomonas savastanoi
and high tolerance to salinity and Veticillium dahliae. This cultivar is distributed
throughout Tunisia, Argentina, Turkey and the USA. Synonyms of this cultivar
are Gerbide, Getlet and Gherlide.
Mission
This cultivar is dual-purpose, i.e. for both table and olive oil production. It is selffertile, with high rooting ability of leafy cuttings under mist, and its tendency for
alternate bearing is intermediate. The tolerance of this cultivar is high to cold,
medium to drought and Pseudomonas savastanoi and low to Aspidiotus hederae,
Cycloconium oleaginum, Gloesporium olivarum, Parlatoria oleae, Saissetia oleae,
Verticillium dahliae and salinity. Mission is distributed throughout Argentina,
Australia, Azerbaijan, Egypt, Iraq, Japan, the USA and other areas.
Olive Varieties
265
Table 23.4. The 14 genotypes of olive cv. Manzanilla.

Genotype
Origin
Manzanilla sevillana
Manzanilla de Carmona
Manzanilla de Tortosa
Manzanilla de Jaen
Manzanilla de Sevilla
Manzanilla dos Hermanas
Manzanilla Hermanas
Manzanilla del Piquito
Manzanilla de Almodovar
Manzanilla Italy
Manzanilla Carrasquena
Manzanilla Commune Argentina
Hass improved Manzanilla
Manzanilla Israel
Spain
Spain
Spain
Spain
Spain
Spain
Spain
Spain
Italy
Portugal
Argentina
Argentina
USA
Israel
Barnea (or K-18)

This is a new cultivar selected in Israel by Professor Shimon Lavee. It was found
in the Kadesh Barnea region, located between the Sinai Desert and Israel. It is
an early producer, giving third-year yields up to 10 t/ha with an oil content of
20%. It is suitable for mechanical harvesting and more than 40 trees/1000 m2
can be planted.
The tree is vigourous, with thin fruiting shoots. The canopy is open. The
leaves are medium to large in size. The fruit is elongated and pointed to the
apex; the stone is banana-shaped. The tree is sensitive to water stress. It is
propagated by leafy cuttings that root easily. The fruits mature in mid-season.
This cultivar is used for oil extraction and for black pickles, and has some
resistance to leaf spot (Cycloconium oleaginum). It is prolic in growth and
fruiting, very productive and with little alternate bearing. The average
production is 1.5 t/1000 m2. The most appropriate pollinator is the cv.
Manzanillo. Barnea trees can be adapted to heavy soils.
Arbequina
Arbequina is an oil-producing cultivar with high oil content, very productive,
producing every year and with a high rooting ability for its leafy cuttings.
Arbequina is very tolerant to cold, salinity and high atmospheric moisture,
moderately tolerant to drought, Pseudomonas savastanoi and poorly tolerant to
Bactrocera oleae, Cycloconium oleaginum, Gloesporium olivarum, iron chlorosis,
Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica, Pratylenchus
266
Chapter 23
penetrans, Pratylenchus vulnus, Saissetia oleae and Verticillium dahliae. Synonyms of

this cultivar include the following: Arbequin, Alberchina, Catalana, Blancal,
Oliva de Arbela, Manglot, Blancas and Oliva de Borjas. The cultivar is
distributed throughout Spain, Algeria, Argentina, Australia, Bolivia, Brazil,
Chile, China, France, Israel, Peru and the USA. It is used for super-high-density
plantings, together with Arbosana and Koroneiki (Larbi et al., 2006). The trees
requirements for chilling time are low, and therefore this cultivar can be
expanded in southern areas with very mild winters.
Saloneque
This variety has medium vigour and adequate productivity. The inorescences
have many owers; the fruit is oval and of medium size. The green ripe fruits
are used for canning.
Tanche
The tree is very vigourous and very tolerant to cold. Its inorescences are short
and compact. The productivity of the tree is good and constant. The fruit is of
medium size, heart-shaped and it is used for canning.
Coratina
This cv. originated in Italy. The tree is of medium size, spherical and with long
shoots. The leaves are elliptical in shape, the fruit weighs 4.5 g and is oval in
shape. The season of ripening is average and its oil content is 2126%. The
cultivar is non-self-fruiting, with high and constant productivity. The best
pollinizers for this cultivar are Frantoio and Moraiolo. It is tolerant to water
stress and cold and it has adapted to varying soil conditions.
Verdale, S.A. Verdale and Wagga Verdale

A synonym of this cultivar is Verdial, and it originated in the southern part of
France. Today there are known many different Verdales all over the world,
differing in fruit size, oil content, esh:pit ratio, hardiness and other
characteristics. The South Australian Verdale has a larger oval fruit (710 g)
than the normal Verdale. The Wagga Verdale has smaller fruit like the
common Verdale, but yields more than the Australian clone. These three
clones are well adapted to South Australian conditions. The oil content of these
clones is low, not exceeding 20%.
Olive Varieties
267
Aglandau
This is an oil-producing cultivar with medium oil content, self-fertile, an
intermediate tendency for alternate bearing and a high rooting ability for leafy
cuttings. The cultivar is considered as being very tolerant to cold and
Pseudomonas savastanoi, moderately tolerant to Bactrocera oleae, drought, Prays
oleae, Saissetia oleae and wind and poorly tolerant to Cycloconium oleaginum. This
cultivar is distributed throughout France, Azerbaijan, Australia and Ukraine
and is also known by various synonyms such as Aglandaou, Aglando, Airane,
Argental, Blancane, Blanchet, Blanquet, Blanquette, Olivier Commun,
Verdale, Verdaou and Luzen.
LARGE-FRUITED OLIVE CULTIVARS

Konservolia (Olea europaea var. med. rotunda)
Known also under the names Voliotiki, Piliou and Amphissis, this is
cultivated in various regions of Greece such as Agrinio, Arta, Amphissa, Pilio,
Stylis, Atalanti and Magnisia. This cultivar is self-fruiting, although planting of
pollinizers such as Chondrolia Chalkidikis, Adramytini, Gordales and
Kalamon increases productivity. The mean tree height is 68 m. The mean
fruit weight is 6.5 g and is mostly used for olive pickling and for black ripe
olives. The fruit is spherical and the esh:pit ratio is 8.28:1. It prefers soils of
medium texture or fertile ones. It can be cultivated at up to 600 m elevation
and it is sensitive to Dacus infection and to frost damage.
Gaidourolia (Olea europaea var. major macrocarpa)

Known also under the names Koromilolia and Damaskinati, the tree has a
height of 56 m, leaves of light green colour and has an elongated fruit with an
average weight of 1012 g. The surface of the fruit has many lenticels, whitish
to light green in colour. The ratio of esh:pit is 9.7:1 and its oil content is 17%.
The main use of this cultivar is in the pickling of green table olives. When the
fruit load is average, the fruit size is very large up to 30 g. This is a promising
variety for pickling green olives.
Karydolia (Olea europaea var. makima)

Other names of this cultivar are Kolymbada, Kolymbati and Karydorachati.
This cultivar is cultivated in some regions of Greece such as Chalkidiki, Fokis
268
Chapter 23
and Attiki. The tree height is 58 m, with light green leaves and
cylindroconical fruits of average weight 58 g, with a teat. The ratio of esh:pit
is 6.6:1 and its oil content is 14%. This is a processing cultivar appropriate for
green and black ripe olives.
Amygdalolia (Olea europaea var. amygdaliformis)

Other names of this cultivar include Stravomita or Kurunolia. It is grown in
some regions of Greece such as Amphissa and Thiva. Its canopy is spherical,
the leaves are broad and lengthy and the fruit weight is 510 g. The ratio of
esh:pit is 5.06.5:1 and its oil content is 820%.
This variety is cultivated mostly in the region of Chalkidiki (northern Greece).
Its fruit is very large and can exceed 10 g in weight. It is sensitive to frost and
non-self-fruiting. The best pollinizers of this cultivar are Amphissis,
Megaritiki, Koroneiki, Manzanillo and Gordales. The main use of this
cultivar is for either pickling of green olives or stuffed (with almond or chili)
green olives. The remaining product inappropriate for processing is used for oil
production. It is very sensitive to Dacus oleae.
Sevillano (syn. Gordal, Gordal Sevillano and Sevillana)

This is a large-fruited variety with 14% oil content (see Fig. 23.3). The trees are
less productive than Manzanillo, with a spreading habit of the shoots. The
cultivar is difcult to adapt and is not easily propagated by cuttings under mist.
The tree requires fertile soils and the fruit weight is 1214 g, with irregular
shape. The esh is not easily separated from the pit. It requires 2000 h at < 7C
to differentiate owering buds. The best pollinizers are the cvs Chondrolia
Chalkidikis, Kolovi, Adramytini and Manzanillo.
The esh:pit ratio is 7.3:1. The fruits are oval in shape, with an indent at
the stem, which can give it a heart-shaped appearance. The skin is thin and
speckled, with white markings. The fruit esh is green, turning purplish black
when ripe and with a good texture for pickling. The pit has deep grooves.
Sevillano occupies second place after Manzanillo in both the Spanish
and Californian table fruit market, where it obtains good prices. Early
harvesting can reduce processing problems, such as bruising and split pits.
Sevillano is resistant to cold, although it requires 2000 h at < 7C in
Spain, and in Italy it is cultivated in warmer regions without problems.
Currently, this cultivar covers 20,000 ha in Spain. Due to high levels of soil N
Olive Varieties
269
Fig. 23.3. The olive cv. Sevillano.
fertilization, Ca uptake is reduced and a problem known as soft nose appears,

i.e. the bottom part of the fruit becomes soft and deteriorates.
This is a table olive cultivar characterized by self-sterility and shows an
intermediate tendency for alternate bearing. Gordal Sevillana shows low
tolerance to Bactrocera oleae, cherry leaf roll virus (CLRV), Camarosporium
dalmatica, drought, Gloesporium olivarum, Meloidogyne arenaria and javanica,
Pratylenchus vulnus, Pseudomonas savastanoi, strawberry latent ringspot virus
(SLRV) and Saissetia oleae and medium tolerance to cold, Cycloconium
oleaginum, salinity and soil moisture.
This cultivar is distributed throughout many regions such as Argentina,
Australia, Israel, Cyprus, Croatia, Italy, Lebanon, Morocco, Spain, France,
Portugal, Chile, the USA, Algeria, Japan, South Africa and Ukraine.
Ascolano
This is a large-fruited variety (810 g) with low oil content (13%). The esh has a
pleasant taste and is easily separated from the pit. The esh:pit ratio is 8.2:1. The
period of ripening is during the middle of October. The tree is vigourous, with a
spreading habit. The cv. Ascolana tenera originated from Ascolano, which is selffruiting and tolerant to Cycloconium, Bacterium tumefaciens and low temperatures.
Ascolana tenera
The tree is vigourous, with a very dense canopy and grows with a spreading
habit. The leaves are medium-sized, elliptical in shape and dark green. The fruit
is ellipsoid and asymmetrical. The mean fruit weight is 810 g and is used for
pickling table olives. The esh comprises 86% of the fruit and its oil content is
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Chapter 23
1618%. Ascolana tenera is a non-self-fruiting cultivar with high percentage

of imperfect owers, average tendency for alternate bearing and a high rooting
ability of leafy cuttings. The best pollinizers for Ascolana tenera are the cvs
Lea, Rosciola, Leccino, Frantoio and Pendolino.
Ascolana tenera is a very tolerant cultivar to cold, cucumber mosaic virus
(CuMV), iron chlorosis, Meloidogyne arenaria and Meloidogyne hapla,
moderately tolerant to drought, Gloesporium olivae, Parlatoria oleae and SLRV
and poorly tolerant to Bactrocera oleae, Cycloconium oleaginum, Gloesporium
olivarum, Meloidogyne incognita, Meloidogyne javanica, Pratylenchus vulnus,
Prays oleae and Saissetia oleae. This cultivar is known also by various synonyms
such as Ascolano, Askolano, Asiolani, Noce, Nociola, Nociu, Oliva
grossa and Spanish Queen. Ascolana tenera is distributed throughout
Argentina, Albania, Croatia, Cyprus, Egypt, France, Italy, Israel, Lebanon,
Pakistan, Slovenia, Serbia, Montenegro, Turkey, Brazil, Chile and the USA.
Santo Agostino
The region of origin of this cultivar is the area of Puglia, in Italy. The tree is
vigourous and the fruit is large (89 g), asymmetric and oval. The fruit is
harvested during September for production of green canned olives and the
period of ripening is November. The fruit esh represents 90% of its weight. The
cultivar is self-sterile and the best pollinizers are the cultivars Moraiolo and
Corregiolo. It resists Cycloconium oleaginum.
Santa Caterina
This originated from Italy. The tree is vigourous and spherical, with light green
leaves. The fruit weighs 79 g, is elliptical and asymmetric in shape. It ripens
early and harvesting starts in September. Its tolerance to cold is adequate and
moderate to Cycloconium oleaginum.
Grossa di Spagna
This also originated in the Puglia region of Italy. The vigour of the tree is
average, with dense shoots. The leaves are of medium size, elliptical in shape
and dark green. The fruit weight is 912 g and its shape is elliptical and
asymmetric. The period of ripening is early and the fruit esh comprises 75% of
the fruit, containing 1619% olive oil. Its productivity is medium to high. The
cultivar is self-sterile and the best pollinizers are the cultivars Maurino,
Coratina, Pentolino, Frantoio and Leccino. It is suitable for green olive
canning and requires fertile and irrigated soils.
24
TABLE OLIVES
INTRODUCTION
Worldwide interest exists in using processing methods for olives such as brine,
fermentation and salt-/heat-drying methods rather than processing with lye
(caustic soda). Primary processing involves any of several operations (soaking,
fermentation, lye treatment or heat). Secondary processing involves increasing
the organoleptic value of the olive by, e.g. addition of herbs, spices or vegetables,
de-stoning and stufng with peppers, cheeses, almonds, garlic or onion.
The most important table olive varieties internationally are Kalamata,
Conservolia, Manzanillo, Sevillana, Hojiblanca and Ascolana tenera.
The Californian table olive industry is based on ve cultivars; in order of
volume sales they are Manzanillo, Sevillano, Mission, Ascolano and
Barouni (Ferguson, 2006). Many olive products have now attained a
worldwide commercial base, i.e.:
Kalamata type.
Green ripe Californian type.
Castelvetrano type (Romeo et al., 2006).
Throumbes.
Sicilian-type green olives.
Picholine type.
Spanish green olives.
A detailed description of table olives is given by Zervakis (2006) and

Kyritsakis (1998, 2007).
BLACK OLIVES IN BRINE

The olives are subjected to a primary process for de-bittering, and this is
followed, after de-stoning, by addition of other materials to the brine such as
olive oil, vinegar, herbs and spices or pimiento and almonds.
271
272
Chapter 24
The optimum quality of table olives is determined by many factors, in

particular the olive grove conditions and the selected variety. The oleuropein
concentration is markedly reduced during processing, leading to de-bittering.
The soluble sugars of olive esh are essential for supporting fermentation
during processing.
Most consumers prefer medium- to large-sized olives, with a esh:stone
ratio 56:1 or more. Freestone varieties are best, since the esh separates easily
from the stone. Olive size, shape, esh:stone ratio, ease of de-stoning, colour
and texture are the most important selection criteria for table olives. Most olive
trees suitable for table olive processing are clonally propagated, with the
exception of difcult-to-root varieties like Kalamata. Some of the most
important varieties for table olive processing include Manzanillo, Sevillana,
Barouni and Hojiblanca for green olives and Conservolia and Kalamata
for black ripe olives. Other varieties well known internationally are Chondrolia
Chalkidikis, Nocellara del Belice and Picholine.
Very few chemicals are required for successful olive cultivation; correct
pruning will help to control many problems; Cu sprays following harvesting
and pruning can be used as a general antifungal treatment.
Table olives should be harvested by hand in preference to mechanical
harvesting, in order to reduce the risk of damaged fruit that, when processed,
produces an inferior product (Balatsouras, 1990). Olive harvesting by hand (or
mechanical) rakes, tree shakers or overhead harvesters increases the risk of
bruising, leading to soft fruits after processing. Mechanical harvesting with
shakers or vibrators has limited application for table olive production.
Ethylene-releasing compounds have also been used to improve fruit abscission,
but their use is limited since that leads to substantial leaf loss. Table olives
should be harvested in three stages: green ripe, turning colour and black ripe.
Harvesting time depends on the variety, growing conditions and crop load.
Green ripe olives are picked from late summer to early autumn, and black ripe
olives from late autumn to late winter.
Green ripe olives are processed as green table olives, the period when they
have attained maximum size. Naturally black ripe olives (those that fully ripen
on the tree) have adequate esh colour and are hand-harvested. To avoid overripening, the optimum stage for harvesting is when the pigments are present in
around two-thirds to three-quarters of the esh.
Raw olives should be processed as quickly as possible after picking, i.e. 12
days, to avoid damage; damaged fruits yield a product of poor quality and taste.
Storage of raw olives at low temperatures decreases the danger of deterioration. A
temperature of 510C may preserve the quality of raw olives for 48 weeks.
However, temperatures < 5C may cause fruit browning. A controlled atmosphere
with low oxygen (2%) and 9095% air humidity prolongs the storage period. The
nutrient levels in the esh of raw olives are as follows: K, 0.533.39%; P,
0.020.25%; Ca, 0.020.16%; Mg, 0.010.06%; S, 0.010.13%; B, 422
mg/kg; Fe, 395 mg/kg; Mn, 0.9155.00 mg/kg; and Zn, 1.433.0 mg/kg.
Table Olives
273
PROCESSING
Raw olives are bitter and require processing in order to become suitable for
consumption. Processing should be conducted under good sanitary practices
in order to maintain all ingredients and all the necessary chemical and
microbiological standards. Processing affects the concentration of the major
compounds, depending on the type of olive (see Table 24.1; Bianchi, 2003).
For high-quality table olives the following requirements are important:
Good-quality water.
Excellent quality of raw olives.
Excellent quality of the chemicals and additives used.
The avour and taste of processed olives depend on the variety,

fermentation, processing solution and packing solutions such as vinegar, olive
oil and avourings.
The equipment required for table olives includes sorters, graders, tanks,
pumps and packing equipment. At every stage in the process sanitation is very
important in safeguarding consumers health, with all statutory food and safety
standards complied with. Table olives are derived from good-quality raw olive
fruits harvested at the appropriate stage of ripening and processed to give a tasty
and safe product. Various table olive processing methods are used, depending on
cultivar, ripeness, cultural condition and technology of processing.
Among the various types of olives destined for the table, the most popular
are the following:
Kalamon, used for Kalamata-type olives.

Spanish-type green olives (Sevillana or Manzanillo).
Greek Conservolia for naturally black ripe olives.
Throumbes best for production of dried olives.
Californian black olives Mission.
Sicilian green olives.
Table 24.1. The effect of processing on the major compounds in three types of
processed table olives (from Bianchi, 2003).
Olive type
Compound
Spanish style
Greek style
Californian style
Triglycerides
Phenols
No change
No change or
decrease
No change or
decrease
Decrease
Decrease
No change
No change
No change or
decrease
No change or
decrease
Decrease
Decrease
No change
No change
Decrease
Triterpene acids
Glucosides
Sugars
Proteins
No change or
decrease
Decrease
Decrease
No change
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Chapter 24
Green ripe olives

These olives are harvested at the yellowish green to straw colour; the fruit
should be rm. Green ripe olives are treated with a lye solution (caustic soda,
NaOH), washed to remove the lye and stored in brine to undergo complete
lactic acid fermentation.
Black ripe olives

Black ripe olives are harvested and processed by placing them into brine for
spontaneous fermentation. Consequently, they are preserved in brine, by
sterilization or pasteurization or by preservatives. Due to processing these
olives lose their intense black colour, which can be restored by exposure to air
after processing.
Shrivelled black olives

This product is achieved with or without lye treatment in brine or by addition
of dry salt. With this treatment the olive esh is dehydrated, resulting in
shrivelled olives. For this process containers allow the liquid from the processed
olives to drain away.
Olives darkened by oxidation

Green ripe olives are de-bittered by an alkaline solution, exposed to the air to
turn black by oxidation, washed free of lye and then packed in containers
containing brine.
Olive products
Such products are marketed as green olive paste, olive rollers, stuffed olives and
marinaded olives.
Bruised olives
These are derived from green ripe olives or those turning colour. They are
crushed in such a way that the esh is exposed, without breaking the stone.
The olives are then de-bittered in water and packed into containers containing
brine, to undergo natural fermentation.
Table Olives
275
Split olives
Black ripe or green ripe olives may be used. The olives are split longitudinally
with a knife or other device, to penetrate the esh. They are then de-bittered
with or without a lye treatment and packed into containers with brine to
undergo natural fermentation. After fermentation, oil, lemon or vinegar can
be added.
Water-cured olives
This method involves immersion in fresh water every day for 10 days, to
achieve de-bittering. With every water change the bitter substance oleuropein
is leached out of the olives and the leaching solution is discarded. Subsequently,
the olives are stored in 10% brine. This method is fast, but it requires
large quantities of water, which create disposal problems with regard to
contamination of the environment. After de-bittering, vinegar or oil may be
added. With this process the traditional Kalamata variety of olive (naturally
black) is produced.
Brine-cured olives
The raw olives are put into containers containing 10% (w/v) sodium chloride
(NaCl). The olives absorb the NaCl and a slow fermentation takes place
(Division of Agricultural Science, University of California, 1975). Over the
following 8 weeks, the concentrations of salt in the fruit and the brining
solution equilibrate. Due to salinity effects the water-soluble oleuropein,
phenolics and minerals leach out from the olive fruits. During this period
fermentation occurs, with the sugars being transformed into lactic and acetic
acids, and other substances. The entire process lasts from 3 to 12 months
depending on the variety, ripening, salt concentration and pH of the brine.
Slicing the olives speeds up the de-bittering process. Ready-forconsumption naturally black olives in brine have a pH of 4.54.8, free acidity
of 0.10.6% w/v (lactic acid) and NaCl of 10% (w/v).
Spanish-type green olives

With this method Spanish-type green olives are produced. Green Spanish-style
table olives contain 928% lipids, 1.01.5% proteins, 1.52.5% bre and
46% ash. Furthermore, these olives contain Na, P, K, Mg, Fe, Mn, Zn, Cu and
vitamins (Garrido-Fernndez et al., 2006). Raw green ripe olives are soaked in
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Chapter 24
tanks containing 12% NaOH (lye) for 812 h for de-bittering. When the NaOH
has penetrated through three-quarters of the esh the lye is removed. The
remaining esh not de-bittered supplies sugars necessary for fermentation.
Penetration of the lye is checked by observing the colour changes following
slicing. Also, by application of phenolophthalein solution to the cut esh the
colour changes from green to red. After de-bittering, the NaOH is removed by
several washings of good-quality water. Subsequently, the tanks are lled with
10% NaCl for lactic acid fermentation. These olives are suitable for human
consumption within 5 weeks. The characteristics of this ready-to-eat product
are: pH 3.84.2, free acidity 0.81.2% w/v (lactic acid) and NaCl 78% (w/v).
The varieties processed by this method include Manzanillo, Sevillana
and Chondrolia Chalkidikis.
Californian-type black ripe olives

The raw product for this purpose is olives that are green or turning in colour.
The fruits are treated with NaOH solutions of different concentrations until the
lye penetrates the esh and the olives are de-bittered. During this process
polyphenols are oxidized and change the fruit colour to brownish black.
Subsequently, the NaOH solution is removed and the treated fruit are immersed
in tanks containing 0.1% ferrous gluconate, to enhance the black colour. After
leaching to remove excess Fe, the fruits are placed in containers containing
23% NaCl and sterilized. The characteristics of ready-to-eat black olives are
pH 58 and NaCl 15% (w/v). With this method the fruit is ready within 12
weeks. This method is very fast and does not require fermentation, but it does
require large volumes of water for both the lye phase and subsequent washings.
Raw olives have a population of natural microora that includes: Gramnegative bacteria, lactic acid bacteria (homofermentative and heterofermentative), yeast, oxidative yeast, Clostridium, Propionibacteria, etc. The
Gram-negative bacteria predominate and produce CO2, which dissolves in the
NaCl solution producing H2CO3, thus lowering pH (see below). This process
creates anaerobic conditions in the brine.
FERMENTATION OF OLIVES
Aerobic fermentation
Aerobic fermentation of olives is based on the action of lactic acid, which is
produced from lactic acid bacteria starter cultures (Marsilio et al., 2005), or
from Lactobacillus and yeasts grown in the sugars released from olives during
processing (Marsilio, 2006). In the process of olive fermentation lactic and
acetic acids are produced; these acids lower the pH of the substrate. The dual
Table Olives
277
action of low pH and high salt concentration signicantly reduces microbial

contamination problems in olives. With fermentation at temperatures of
2025C, avour-giving compounds are produced.
Anaerobic fermentation
Another process used in table olive production is anaerobic fermentation,
either as an initial process or after de-bittering of the olives by the application of
lye. With this process the organic substrates of the fruits are broken down to
produce lactic acid. Mature olives are habituated by homofermentative and
heterofermentative lactic acid bacteria and yeasts.
The following Gram-negative bacteria can be found in olives:
Lactic acid bacteria.

Clostridium.
Propionibacterium.
Bacillus.
These bacteria ferment sugars and liberate CO2; the CO2 dissolves in the
brine and produces H2CO3, which lowers the pH to about 5. The duration of
this process is 34 days. The same chemical process is achieved by the addition
of lactic acid. The lowering of pH and the established anaerobic conditions
prevent the further growth of Gram-negative bacteria, and the product is
therefore stabilized. Processed olives should maintain some bitterness and fruit
avour. Yeasts and moulds are also natural inhabitants of the olive fruit.
SECONDARY PROCESSING, PACKING AND LABELLING OF

OLIVE PRODUCTS
Secondary processing involves the stufng of olives with various food products
to achieve specic avours. Some of these additional avours include vinegar,
olive oil, herbs and spices (oregano, garlic, etc.). Furthermore, olives can be
stuffed with chili, peppers, cheeses, pimiento, almonds, sh, etc. Among these
the most common use is that of vinegar, which lowers the pH and thus
increases shelf life. Various types of vinegars are used, such as wine vinegar,
cider vinegar or balsamic vinegar. In green olives we prefer the light-coloured
vinegars, while in black olives darker vinegars are used.
For packing processed olives a brine solution of 810% is used. In olives
containing vinegar the brine solution contains three parts of brine plus one of
vinegar. To avoid spoilage the surface of olive containers is covered with olive oil.
Table olives must be labelled appropriately. Labelling information should
include caloric value and the content of various substances such as
carbohydrate, oil, protein and minerals.
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Chapter 24
RULES FOR THE PRODUCTION OF HIGH-QUALITY

TABLE OLIVES
Use of good-quality water, for both processing and washing.

Use of high-quality raw olives, chemicals and stufng materials.
Adherence to all hygiene rules in the processing unit.
The processed olives should be of the recommended cultivar, with no
defects and of appropriate marketable size.
The table olive processing facility should follow all statutory regulations
regarding food, health and protection of the environment. The processing unit
is comprised of the following areas:
Reception and grading of fresh olives.

De-bittering of olives.
Primary and secondary processing.
Packing of the processed product.
Storage of chemicals.
Storage of packed products.
Analytical laboratory.
Loading, marketing and ofces.
STORAGE OF FRESH OLIVES

After harvesting, olives should immediately be processed, in order to avoid
deterioration. After 1 weeks storage at room temperature olives start to
deteriorate quickly due to the presence of bacteria, yeasts and certain enzymes
present in the esh. Therefore, if processing is not to be carried out
immediately, fruits should be refrigerated. During storage olives lose weight,
and shrivelling may occur. Storage at 510C can extend the olive life for
48 weeks. However, temperatures < 5C cause esh browning. Ideal
conditions for olive storage are under a controlled atmosphere of 5.07.5%
CO2, 2% O2 and 9095% relative humidity.
25
OLIVE OIL
EXTRACTION
For olive oil extraction three systems are available:
The traditional discontinuous processing system.

Continuous-cycle centrifugation.
The percolationcentrifugation system.
Olive production involves the twin stages of milling and separation of the
oil from water, resulting in three constituents oil, husk (solid waste) and
waste water. In the traditional method milling is conducted with a millstone or
hammer stone, and oil extraction is achieved by hydraulic pressure.
In the continuous cycle, grinding of the olives is achieved by metal
crushers, and subsequently the olive paste is centrifuged with a horizontal
centrifugal decanter. Afterwards, the oil is separated from waste water by a
vertical centrifuge. Extra virgin olive oil is produced by cold pressing of the olive
paste. The process of oil extraction inuences the phenolic content of the oil.
Oils obtained by centrifugation have a lower phenolic content, since this
method uses large quantities of water, thus removing a signicant proportion
of the phenols. Furthermore, the type of mill used for oil production exerts a
signicant inuence, and hammer mills are more efcient in extraction of
phenol.
The phenolic composition of olive oils is the result of a complex interaction
between several factors, which include cultivar, stage of maturation, climate,
mill as crushing machine and malaxation conditions. Malaxation is the action
of slowly churning or mixing milled olives in a specially designed mixer for
2040 min. This churning allows the smaller droplets of oil released by the
milling process to aggregate and be more easily separated. The paste is
normally heated to around 27C during this process.
279
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Chapter 25
CATEGORIES OF OILS AND STONE OILS

The characteristics of olive oil are described in EU Regulation 2568/91. These
characteristics are given in Table 25.1.
Virgin olive oil

This is produced by mechanical or physical processes and relatively low
temperatures that cause no deterioration in the oil. This product is exposed to
no other processes than those of fruit cleaning, centrifugation and ltration.
Virgin olive oil is divided into the following categories:
Extra virgin olive oil
This is a virgin olive oil containing free fatty acids (expressed as oleic acid) 1
g/100 g (see Table 25.1). Its other characteristics are those of virgin olive oil.
Virgin olive oil
The free fatty acid content (expressed as oleic acid) is 2 g/100 g. Its other
characteristics (colour, avour, taste, etc.) are those as in the other virgin olive
oil categories.
Curante virgin olive oil
The free fatty acid content is 3.3 g/100 g. All the other characteristics are
those of virgin olive oil.
Lampante virgin olive oil
The free fatty acid content is > 3.3 g/100 g.
Table 25.1. The main characteristics in the categorization of olive oil, according to
EU Regulation 2568/91.
Oil
category
Extra virgin olive oil
Virgin olive oil
Curante virgin olive oil
Lampante virgin olive oil
Rened olive oil
Olive oil
Non-processed pomace oil
Rened pomace oil
Pomace olive oil
Free
acidity (%)
1.0
2.0
3.3
> 3.3
0.5
1.5
0.5
0.5
1.5
Peroxide
index
20
20
20
> 20
5
15
5
15
Waxes
(mg/kg)
250
250
250
350
350
350
> 350
Olive Oil
281
Rened olive oil

Produced from virgin olive oil in reneries, and its free fatty acid content (as
oleic acid) is 0.5 g/ 100 g.
Olive oil
This is a combination product of the mixing of rened olive oil with virgin olive
oil (not lampante) with free acidity 1.5 g/100 g.
Crude pomace oil
Produced after extraction of olive pomace with an organic solvent, and not t
for human consumption. EU law denes any oil containing 300350 mg/kg of
waxes, and aliphatic alcohols > 350 mg/kg, as crude pomace oil.
Rened pomace oil
The pomace oil after rening, giving a product not t for human consumption,
with free acidity 0.5%; its other characteristics must conform to the
standards for this category.
Pomace olive oil
This is a mixture of rened stone oil and virgin olive oil (except lampante), t for
human consumption; its free acidity level is 1.5%
The categories of olive oil that can be sold in the retail market are extra
virgin olive oil, virgin olive oil, olive oil and pomace olive oil.
STORAGE AND BOTTLING

The high-quality oils are stored in stainless steel containers at a constant
temperature of 719C. Both storage period and temperature affect olive oil
quality (Pereira et al., 2002; Clodoveo et al., 2007), and especially oil and
residual DNA (Luna et al., 2006; Spaniolas et al., 2007). After extraction the oil
is stored in large stainless steel tanks for more than 1 month, in order to settle
out the water and pulp. Decanting follows, in order to remove sediments that
otherwise would appear in the bottled product; it also eliminates the presence
of processing water, which could give rise to off avours.
As soon as the producer has an order for a certain number of bottles the oil
is bottled and sent to the market, and should be consumed within a short
period in order to avoid deterioration of quality within the bottle.
SENSORY EVALUATION OF QUALITY

Olive oil should have a fruity avour that characterizes the variety or the mixture
of varieties from which that oil was produced. The quality can be evaluated for
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Chapter 25
certain characteristics with equipment or by sensory analysis, which could be

much more accurate. With sensory evaluation the aroma, taste and textural
changes are easily evaluated. Sensory evaluation determines not only defects due
to improper storage or processing but also the positive characteristics of the oil.
Among these characteristics, bitterness, pungency and fruity olive avour are
included. Bitterness and pungency are frequent in olive oils following fruit
processing. These are not defects, but important characteristics that are related
to the long shelf life of olive oil. Bitter and pungent oils have greater polyphenol
content and can be stored for a longer period than sweet oils.
CRITERIA OF QUALITY AND STANDARD VALUES

Certain laboratory measurements are used to determine adultery by seed oils,
and also the levels of free acidity and peroxide that indicate the degree of
oxidation in an olive oil. Due to oxidation olive oil develops off-avours and
turns rancid. Table 25.2 shows the quality criteria and standards for olive oil
(according to EU Regulation 2568/91) and Table 25.3 denes the 100C purity
and authenticity criteria (sterols, fatty acids, saturated fatty acids) for olive oil.
CERTIFICATION
A great number of certications exist, the two most important of which, in the
context of olives, are described below.
Protected Designation of Origin (PDO)

Determination of the geographical origin of an olive oil can be conducted
using 13C nuclear magnetic resonance (NMR) (Vlahov et al., 2003), NMR
Table 25.2. Quality criteria and standards for olive oil, according to EU Regulation
2568/91.
Oil category
Extra virgin oil
Virgin oil
Ordinary virgin oil
Lampante oil
Rened olive oil
Olive oil
Free
acidity (%)
Peroxide
index
K270
K232
K
Mean
defects
0.8
2.0
3.3
> 3.3
0.3
1.0
20
20
20
5
15
0.22
0.25
0.25
1.10
0.90
2.5
2.5
2.60
0.01
0.01
0.01
0.16
0.15
0
2.5
2.56.0
> 6.0
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283
Table 25.3. Purity and authenticity standards for olive oil (from IOOC data,
www.cesonoma.ucdavis.edu/hortic).
Content and composition
Individual sterols (mg/kg)
Cholesterol
Campesterol
Stigmasterol
D-7-stigmasterol
-sitosterol
Total sterols (mg/kg)
Fatty acids composition f
Myristic acid, C14:0
Palmitic acid, C16:0
Palmitoleic acid, C16:1
Heptadecenoic acid, C17:0
Heptadecenoic acid, C17:1
Steatic acid, C18:0
Oleic acid, C18:1
Linoleic acid, C18:2
Linoleic acid, C18:3
Arachidic acid, C20:0
Gadoleic acid, C20:1
Behenic acid, C22:0
Saturated fatty acids (%)
Statutory level
Max. 0.5a,b
Max. 4.0a,b
Max. 0.5a.b
Min. 93a,b
Min. 1600b, 1000c, 2500d, 1800e
Max. 0.05a,b
7.520a,b
0.33.5a,b
Max. 0.3a,b
Max. 0.3a,b
0.55.0a,b
55.083.0a,b
3.521.0a,b
Max. 1.0 (EU = 0.3)a,b
Max. 0.6a,b
Max. 0.4a,b
Max. 0.2a,b
Max. 1.5 (EU = 1.3)a,c,e
Max. 1.8 (EU = 1.5)e
Max. 1.8 (EU = 1.5)a
Max. 2.2 (EU = 2.0)d
Max. 2.2 (EU = 2.0)b
aOlive oil; brened pomace oil; cvirgin oil; dcrude pomace oil; erened olive oil; f% of
methylesters.
spectroscopy (Mannina et al., 2001) or sterol composition (Alves et al., 2005).

This denition refers to olive oil produced in a specic region using traditional
production methods from the dened area, of whose name the product bears.
The area of origin affects virgin olive oil quality (Ranalli et al., 1999).
Protected Geographical Indication (PGI)

This indicates that the olive oil is produced in the geographical region whose
name is written on the bottle, allowing for a more exible link to certain regions
having a specic characteristic.
284
Chapter 25
CRITERIA FOR DETERMINATION OF QUALITY

The International Olive Oil Council (IOOC) has established certain criteria in
determining oil quality, such as acidity, oxidation, colour and organoleptic
characteristics.
Free acidity
Free acidity is an indicator of the quality of olives and of the procedures of
harvesting, handling, transportation and storage prior to olive milling. Free
acidity measures the hydrolytic breakdown of triglycerides to di- and
monoglycerides, leading to fatty acid liberation. It is expressed as percentage
free fatty acids especially on the basis of oleic acid, which is the main fatty
acid in olive oil.
Peroxide index
Peroxide index is an indicator of oxidation, the means of creating peroxides in
olive oil. High peroxide values mean that the olive oil is defective and that the
olives were not correctly treated before oil extraction. Peroxide values are
expressed in milliequivalents (meq)/kg of free O2 in oil. Measurement is
conducted by titration with potassium iodide, which liberates iodine.
Ultraviolet (UV) light absorbance

This is also an indicator of oxidation, and in particular for oils that have been
heated during the process of renement in the processing plant. With this
process the absorbance of certain oxidized compounds, at 232 and 270 nm, is
measured. The value ()K detects treatment by decolorizing agents and also
adulteration by rened or pomace oil Optical Density (OD270 and
OD266OD274).
Flashpoint
The parameter of ashpoint measures the temperature at which the oil begins
to burn. The ashpoint in rened olive oil, pomace oil and seed oils is
190200C, while in virgin oils it is 210220C.
Olive Oil
285
Insoluble impurities
These provide an index of the presence of impurities, such as alkaline soaps of
palmitic and stearic acids, proteins suspended in the oil, dirt, oxidized fatty
acids, resins and mineral elements. Insoluble impurities are expressed as a
percentage and are determined by dissolving oil in petroleum ether, and
subsequently by ltration of impurities.
Colour
The type of lipophilic pigments present in the olive oil at harvesting, such as
chlorophylls or carotenoids, determines its colour.
Flavour
The avour is due to the presence of volatile substances, organic acids such as
oleic and linoleic, and also polyphenols.
The quality of oil depends on both the microclimate of the olive grove and
anthropogenic factors. The organoleptic characteristics (avour, aroma) and
the colour depend on soil and climatic conditions and also on the olive cultivar.
The time of olive harvesting also determines oil quality. Harvesting should be
completed quickly, and so too should processing for oil production. In that way,
any potential problems due to Dacus oleae, fruit deterioration or oxidation are
reduced. Reduction in the use of phytoprotective chemicals and replacing them
by biological means improves oil quality. Finally, signicant improvement in
olive oil quality has been achieved by reducing the time from harvesting to
extraction in the mills.
Olive oil has a high biological and nutritional value. However, it can be
polluted with certain chemicals dangerous to human health during the stages
of extraction, rening, bottling and marketing. These include the following:
Agricultural phytoprotective chemicals.

Volatile substances and Freon due to its leakage from refrigerators located
adjacent to olive mills or bottling units.
Heavy metals, due to contact of oil with the metal parts of machinery or
storage tanks.
Polycylic or aromatic hydrocarbons, in the case of pomace oil.
Environmental pollutants such as dioxins and toluene.
286
Chapter 25
AUTHENTICITY
Authenticity means that the oil purchased by the consumer matches its
description. Undeclared addition of vegetable oils and false statements
regarding the source of the oil (geographic origin, cultivar, organic olive oil) are
examples of adulteration (Bazakos, 2007). Growers prefer the production of
superior-quality olive oil, since they can market it more easily and at higher
prices. Food distributors also want their products to comply with current
legislation. Furthermore, most consumers are ready to pay more for olive oil
they consider of high quality derived from cultivation practices, method of
processing, cultivar or geographic origin.
DETECTION OF ADULTERATION
Detection of adulteration is possible by the use of certain analytical methods.
However, adulteration methods are constantly changing and there is always a
need for reassessment of these techniques. Nowadays, various conventional or
non-conventional methods have been developed for detection of adulteration.
Conventional methods are those not based on DNA analysis.
Conventional methods
These methods are based on the analysis of various constituents and properties
of olive oil. Olive oil could be adulterated by other vegetable oils such as pomace
oil, maize oil, groundnut oil, sunower oil, soybean oil, sesame oil, animal
lipids or other sources.
Conventional control of olive oil adultery includes the following methods.
Determination of physical and chemical constants and chemical
compounds of olive oil (Aparicio et al., 1997)
These constants include specic gravity, iodine number, refraction index and
chemometric procedures (Bucci et al., 2002). They are increased by any
adulteration by groundnut oil, cotton oil, soybean oil, sunower oil, maize oil
or sesame oil.
Changes in squalene concentration
The accepted level of squalene (a natural organic compound) is 136708
mg/100g of olive oil. Adulteration shows values outside this range.
Ultraviolet (UV) absorption
Ultraviolet absorption in vegetable oils, at 208210 nm, is three times greater
than that of olive oil.
Olive Oil
287
Gas or HPLC and inltrated spectroscopy

These methods are based on a carrier gas movement through a column (gas) to
generation of a gradient of two solvents (HPLC) and to spectroscopy.
Sterol measurement
High concentrations of the sterol stigmasterol indicate adulteration of olive oil
with soybean oil. The following techniques are available.
NEAR-INFRARED (NIF)
This method utilizes the NIF part of the light spectrum.

NIF causes inter-atomic vibrations of molecules at a frequency of 10131014
Hz. The NIF measurement is a fast, continuous and non-destructive process.
NUCLEAR MAGNETIC RESONANCE (NMR)
This high-resolution method provides

information on liquid or semi-solid materials, involving the use of 1H and 13C
(Vlahov et al., 2003).
ULTRAVIOLET (UV), VISIBLE (VIS) AND INFRARED (IR) OR FTIR SPECTROSCOPY Ultraviolet,
VIS, IR and Fourier Transform Infrared (FTIR) electromagnetic radiation all
interact with various materials which, upon excitation, emit light that can be
measured.
The techniques described above represent non-destructive spectroscopic
methods used to distinguish authentic and non-authentic olive oil samples
(Tapp et al., 2003).
CHROMATOGRAPHY Various forms of chromatography are used, such as gas
chromatography (GC), liquid chromatography (LC), thin-layer chromatography
(TLC) and high-performance liquid chromatography (HPLC). The stationary
phase consists of a thin layer of liquid held in place on the surface of a porous
inert solid. A wide variation of liquid combinations are suitable. The movement
of a mobile phase results is a differential migration and separation of the sample
components. The stationary phase can be either a solid or liquid and the mobile
phase can be liquid or gas (liquidsolid, LSC, or liquidliquid, LLC).
These methods achieve a rapid and reliable separation of molecules with
similar characteristics, based on partition of the molecules between a mobile
and a stationary phase. They are used for the detection of certain adulterants
or to determine oil authenticity. The principles of these methods are:
GC: there is a stationary solid phase and a mobile gas phase.

TLC: there is a solid planar surface and the liquid phase is mobile through
capillary forces.
HPLC: there is a column lled with the stationary phase and a mobile
liquid phase.
These include assessment of enzyme activity by measuring

the rate of consumption of the reactants or the formation of products. These
methods allow quick and accurate determination of certain compounds.
ENZYMATIC METHODS
288
Chapter 25
Within the enzymatic methods are included immunoassay, which is based

on the ability of antibodies (immunoglobulins) to interact with various
substances. This method involves at least one antibody having specicity for a
particular antigen. When the antigen is immobilized a detection antibody is
added, which forms a complex with the antigen.
Non-conventional methods
The residual DNA of olive oil can be used to determine olive oil authenticity
(Cresti et al., 1996; Muzzalupo et al., 2007; Spaniolas et al., 2008b).
DNA polymorphism
This is dened as genetic changes in the DNA of certain materials, i.e. the genome
of the nucleus, mitochondria or ribosomes, and also of other organelles. Molecular
markers provide information about variations in the DNA sequence. The main
molecular markers are RAPD, SCAR, AFLP, SSR, SNP and intron polymorphism
(Spaniolas et al., 2008a). These markers can be used to determine olive oil
authenticity. A short description of these markers is now given.
RAPD (Random Amplied Polymorphic DNA)
This marker provides a fast and low-cost method, and also requires only a small
amount of DNA. It uses one or two primers in order to provide information on
the multi-banded ngerprints of a genome.
SCAR (Sequence Characterized Regions)
A method involving two specic primers that amplify a well-dened genetic
locus, which is derived by sequencing a single RAPD amplicon. The
polymorphism between SCAR bands is determined by variation in length of the
sequence between the two primes.
AFLP (Amplied Fragment Length Polymorphism)
The origin of polymorphism in AFLP is the same as that of RFLP, i.e. changes in
restriction site sequence, deletions or insertions between two adjacent
restriction sites.
SSR (Simple Sequence Repeats)
These markers are also known as microsatellites and consist of 110 bp, which
are repeated in tandem; the number of repetitions is variable (Carriero et al.,
2002).
Olive Oil
289
SNP (Single Nucleotide Polymorphism)

A technique involving changes of a single base in the DNA sequence that can
be identied by polymerase chain reaction (PCR) (Lavee and Haskal, 1993;
Marmiroli et al., 2003; Spaniolas, 2007; Spaniolas et al., 2007). This method
does not provide a lot of information. Therefore, several adjacent SNPs can be
studied in one sequence. Many types of SNPs are known, such as exon, intron
and promoter, based on the SNP position in a gene. Other types include
regulatory SNPs (rSNPs). Single-nucleotide polymorphism is the most
common DNA polymorphism, which offers the possibility of developing genetic
maps to study various genes.
ISSR (Inter-simple Sequence Repeats)
This technique does not require any prior knowledge of the genomic sequence.
It is a PCR system making use of only one primer, which is complementary to
the 5 or 3 end of a microsatellite region and further extends into the anking
region, thus allowing the amplication of regions found between inversely
oriented repeats. It is a multilocus marker system useful for ngerprinting,
diversity analysis and genome mapping (Godwin et al., 1997).
Of the markers above, RAPD, SCAR, AFLP, SSR, ISSR and SNP can be used
in tandem with PCR analysis.
ADULTERATION OF OLIVE OIL

The high price of olive oil in comparison with that of vegetable oils increases the
likelihood of adulteration, which takes on various guises.
1. Mixing of extra virgin olive oil with low-quality olive oil or vegetable oils.
2. Exposure to treatments other than those required for its production.
Processes such as decolorization, de-odouring or neutralizing acidity are
examples.
3. Designation of geographic origin. Olive oils from designated geographical
areas are, by denition, of high quality; designation of the area of origin
commands higher prices. Details of the particular cultivar from which the oil
has been produced will be written on the bottle label. Olive oils labelled as
carrying designation of origin (PDO) and/or geographical indication (PGI) are
protected by EEC regulation 510/2006. Any oil sourced from outside these
criteria but purporting to be either PDO or PGI is classied as having been
adulterated.
Many chemical and biochemical methods have been developed in order to
determine the authenticity of olive oil, and today the methods of DNA analysis
are more important, since the classical techniques cannot easily distinguish
between closely related materials. Furthermore, conventional chemical
290
Chapter 25
methods are not always able to detect the region of origin of olive oil, since
environment affects chemical composition and phenotype. Molecular markers
can distinguish or identify cultivars, estimate germplasm variability and trace
olive origin. Molecular markers are not affected by the environment and
can help resolve problems such as nursery certication, variety identity,
geographic origin and identication of germplasm collections; many olive
cultivars are screened by Random Amplied Polymorphic DNA (RAPD). The
SSRs (Simple Sequence Repeats) are of small size and can be detected in
degraded DNA, as can DNA extracted from olive oil. Amplied fragment length
polymorphism and microsatellites have also been used. However, a reliable and
promising molecular marker that can signicantly contribute to olive oil
characterization is the single nucleotide polymorphism (SNP). In olives, SNPs
have been used to classify olive cultivars and can differentiate 72% of the olive
samples tested.
From the 60 Greek varieties 43 have been sequenced. According to the
SNP database of the Greek olive varieties, an initial differentiation was carried
out. The SNP data resulted from lupeol synthase and cycloartenol synthase
targets. However, more genes are required to be sequenced and analysed. By
this technique 19 of the 43 varieties can efciently be tested for adulteration.
These varieties are given in Table 25.4: Amssis, Asprolia Lefkados,
Adramytini, Agouromanakolia, Gaidourelia, Kalokairida, Koutsourelia,
Koroneiki, Kothreiki, Klonares Koropiou, Kerkyras, Mastoides,
Rachati, Thiaki, Tragolia, Throumbolia, Strogylolia, Valanolia and
Vasilikada. In some varieties only one SNP is enough for their differentiation.
Furthermore, classication can be performed in some varieties in relation to
the region of origin. Hence, Adramytini from the Greek island Lesbos can be
differentiated from that from Agios Mamas (northern Greece) through the
SNP(13) of lupeol synthase. Also, Koroneiki and Mastoides from Chania can
be differentiated from Koroneiki and Mastoides from Agios Mamas through
SNP(6) and SNP(2) of lupeol synthase, respectively. The same differentiation is
possible in many other varieties, such as Gaidourelia, Agouromanakolia, etc.
Some groups of varieties have the same SNPs and cannot be differentiated, e.g.
Chondrolia Chalkidikis and Karydolia Chalkidikis.
The varieties Amygdalolia and Kalamon exhibit no difference in SNP
positions, but they can be differentiated by SNP(1), SNP(2), SNP(2+) of lupeol
synthase and SNP(6), SNP(8), SNP(9) and SNP(10) of cycloartenol synthase.
Table 25.5 presents the Greek SNP database of lupeol synthase (lupeol 2Flupeol 2R).
Olive Oil
291
Table 25.4. Differentiation of olive varieties according to their SNP position (from
Bazakos, 2007).
Olive variety
SNP position
Amssis
Asprolia Lefkados
Adramytini (Lesbos)
Agouromanakolia (NAGREF Chania)
Gaidourelia (NAGREF Chania)
Gaidourelia (Kostelenos)
Kalokairida
Koutsourelia (NAGREF Chania)
Koutsourelia (Kostelenos)
Koroneiki (NAGREF Chania)
Koroneiki (Kostelenos and NAGREF
Agios Mamas)
Kothreiki (NAGREF Agios Mamas)
Klonares Koropiou
Kerkyras
Mastoides (Metzidakis)
Mastoides (Kostelenos and NAGREF
Agios Mamas)
Rachati
Thiaki
Tragolia
Throumbolia
Strogylolia
Valanolia (Lesbos)
Valanolia (Kostelenos and NAGREF
Agios Mamas)
Vasilikada
Chondrolia Chalkidikis, Karydolia
Chalkidikis
Konservolia, Dafnelia
Zakinthou, Lianolia Kerkyras
Amygdalolia, Kalamon
Galatistas, Dopia Spetson
Throumbolia (NAGREF Chania),
Kolireiki, Karolia, Matolia,
GPL, Kolympada, Karydolia
Agouromanakolia (Kostelenos),
Adramytini (Kostelenos, NAGREF
Chania, NAGREF Agios Mamas),
Manaki (Kostelenos, NAGREF Agios
ios Mamas), Smertolia, Throumbolia
(NAGREF Chania)
1,2,3 (lupeol2) and 15 (cyclo3)

1 (lupeol2) and 1 (cyclo2)
1,5,8 (lupeol2) and 3 (cyclo2)
2,7,8 (lupeol2)
1,2,7,8 (lupeol2)
2,2+,3,7 (lupeol2) and 6,7 (cyclo2)
1,2,2+,7,8 (lupeol2) and 5,6,7 (cyclo2)
6,7,8 (lupeol2)
6,7 (cyclo2)
6 (lupeol2)
6,3 (lupeol2) and 4,9 (cyclo2)
6 (cyclo2)
1,2,2+ (lupeol2) and 9 (cyclo2)
1,2,2+ (lupeol2) and 8,9,10 (cyclo2)
2 (lupeol2)
1,6 (lupeol2) and 3 (cyclo2)
1 (cyclo2)
1,2,7,8,9 (lupeol2) and 4,5,6 (cyclo2)
1,3 (lupeol2)
1,2,7,8,9 (lupeol2) and 4,5,6 (cyclo2)
5,6 (lupeol2)
1,2,7,8,9 (lupeol2) and 4,5,6 (cyclo2)
6,7,8 (lupeol2)
1,2,2+ (lupeol2) and 6,8,9,10 (cyclo2)
7,8,9 (lupeol2) and 1,2 (cyclo2)
1,2,3,6 (lupeol2), 6,9 (cyclo2) and 15 (cyclo3)
1,2,2+ (lupeol2) and 6,8,9,10 (cyclo2)
All SNP positions
Koroneiki (NAGREF Agios Mamas)
CT
AC?
AG
AT
AA
GG
AC?
CC
GG
AG
Chapter 25
Koroneiki (Kostelenos)
CC
TC
CC
AG
AT
AA
GG
AC
CC
GG
AG
13
21
22
23
Koutsourelia (NAGREF Chania)
Konservolia (NAGREF Chania)
Kerkyras (NAGREF Chania)
Koroneiki (NAGREF Chania)
TC
CA?
GA?
AT?
AA
GG
AC
TC
GG
AG
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
GC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CC
CC
TC
AA
AA
AG?
AA
TC
GA
Dafnelia (Kostelenos)
Gaidourelia (NAGREF Chania)
Amssis (NAGREF Agios Mamas)
Amssis (Kostelenos)
Asprolia Lefkados (Kostelenos)
Chondrolia (Polygyros)
Chondrolia (NAGREF Chania)
Amygdalolia (NAGREF Agios Mamas)
CC
CT
CA
AA
AT
AA
GG
AC
TC
GG
AA
Adramytini (NAGREF Agios Mamas)
AA
AA
AG
AA
TT
GA
Adramytini (NAGREF Chania)
Agouromanakolia (Kostelenos)
Adramytini (Lesbos)
Agouromanakolia (NAGREF Chania)

SNP No.
AA
AA AA
AA AA
AG? GG
AA AA
TT TT
GA GG
Adramytini (Kostelenos)
Amygdalolia (Kostellenos)
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
AA
AA
GG
AA
TT
GG
AA
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
AA
AA
GG
AC
TC
GG
Chondrolia Chalkidikis (NAGREF Agios Mamas)
AA?
AA
AG?
AC
TC
GA
CC
TC
CA
AA
AT
AA
GG
AC
TC
GG
CC
TC
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CG?
CT
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
1
2
2+
3
3
4
5
6
7
8
9
CC
CC
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
TC
CC
CT
CA
AA
AT
AA
GG
AC
CT
GG
AA
Gaidourelia (Kostelenos)
12
20
Genotype not yet named GPL
AA
AG
AG
CC
CC
GA
25
11
19
24
10
Galatistas (Kostelenos)
14
9
18
16
17
15
14
8
7
6
5
13
12
11
10
9
8
7
6
4
3
2
1
5
4
2
3
1
A/A
292
Table 25.5. Greek SNP database of the lupeol synthase (lupeol2F-lupeol2R) fragment (from Bazakos, 2007).
Table 25.5. Continued.
Matolia (Kostelenos)
Megaritiki (Kostelenos)
Megaritiki (NAGREF Agios Mamas)
Dopia Spetson (Kostelenos)

CC?
CT
CA
AG?
AT
AA
GG
AC
CT
GG
AG
GG
AC
TC
GG
AG
293
Mastoides (NAGREF Agios Mamas)
CC
CT
CC
AG
AT
AA
GG
AC
TC
GG
AG
Olive Oil
Mastoides B (Kostelenos)
GC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
Karydolia Chalkidikis (NAGREF Agios Mamas)
Karydolia Chalkidikis (Kostelenos)
Klonares (Kostelenos)
Karolia (Kostelenos)
Kolireiki (Kostelenos)
Lianolia Kerkyras (Kostelenos)
Lianolia Kerkyras (NAGREF Agios Mamas)
CC
CT
AC
AA
AT
AA
GG
AC
CT
GG
AA
CC
CT
CA
AA
AT
AA
GG
AC
CT
GG
AA
GC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
AA
AA
AA
GG
AA
TC
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
CT
GG
AA
Mastoides (NAGREF Chania)
Mastoides A (Kostelenos)
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
39
AA
AA
AG?
AA
TT
GA
GC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
38
CG
TT
27
26
48
47
46
45
44
43
42
22
37
41
21
36
29
Kolymbada (Kostelenos)
Kalokairida (Kostelenos)
Karydolia (Kostelenos)
CC
CC
AA
AA
GG
AA
TT
GG
AA
AA
AA
GG
AA
TT
GG
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CG
CC
CC
SNP No.
Kalamon (Kostelenos)
Kalamon (NAGREF Agios Mamas)
Manaki (Kostelenos)
Kothreiki (NAGREF Agios Mamas)
Manaki (NAGREF Agios Mamas)
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CT
CA
AG
AT
AA
GG
AC
CT
GG
AG
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
AA
AA
GG
AA
TT
GG
1
2
2+
3
3
4
5
6
7
8
9
32
19
40
25
24
23
20
35
34
18
31
33
17
16
15
30
28
27
26
A/A
60
Valanolia (NAGREF Agios Mamas)
Vasilikada (Kostelenos)
Vasilikada (NAGREF Agios Mamas)
Zakynthou (Kostelenos)
Chapter 25
Valanolia (Kostelenos)
Throumbolia (Kostelenos)
Throumbolia (Agios Mamas)
Tragolia (Kostelenos)
Valanolia (Lesvos)
CC
CC
CC
AA
AA
AA
GG
AA
TC
GG
AA
Thiaki (Kostelenos)
CC
CC
CC
AA
AA
AA
GG
AA
CT
GG
AA
AT
AA
AG
AC
TC
GA
CC
CC
CC
AA
AA
AA
GG
AA
TC
GG
AA
AA
AA
GG
AA
TT
GG
CG
CT
CA
AA
AT
AA
GG
AC
CT
GG
AA
31
32
33
51
52
53
54
55
Nissiotiki A (Kostelenos)
Patrini (NAGREF Agios Mamas)
Pikrolia (Kostelenos)
Smertolia A (Kostelenos)
Strogylolia (Kostelenos)
Throumbolia (NAGREF Chania)
1
2
2+
3
3
4
5
6
7
8
9
CT
CA? CC
AG AA
AT AA
AA AA
GG GG
AC AA
CC CC
GG GG
AG
CC
CC
CC
AA
AA
AG?
GG
AA
CC
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC?
CC?
CA?
AA
AA?
AA
GG
AC?
TC
GG
AA
Rachati (Kostelenos)
30
SNP No.
AA
AA
GG
AA
TT
GG
AA
59
CG
TC
CA
AA
AT
AA
GG
AC
TC
GG
AA
CC
CC
CC
AA
AA
AA
GG
AA
TC
GG
AA
CG
TC
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
CC
CG
CC
CC
AA
AA
AA
GG
AA
TT
GG
AA
CC
65
64
63
62
39
38
37
36
61
35
57
58
34
29
50
56
28
49
A/A
CG?
CC
CC
AA
AA
AA?
GG
AC?
294
Table 25.5. Continued.
26
OLIVE MILL PRODUCTS AND
ENVIRONMENTAL IMPACT OF
OLIVE OIL PRODUCTION
OLIVE GROWING AND ENVIRONMENTAL PROTECTION
Olive cultivation has a positive impact on the environment and maintenance of
landscape. Olive culture also helps combat desertication, one of the biggest
problems in the Mediterranean area. Furthermore, the olive tree gives shelter
and food to wildlife. There are, however, instances where olive growing
damages the environment. The intensication of olive growing is accompanied
by increased input of fertilizers, insecticides, herbicides and irrigation water.
Such systematic removal of vegetation by various means has an adverse effect
on biodiversity and increases the loss of soil organic matter. This leads to
environmental deterioration and erosion or desertication.
The environmental problems associated with olive oil extraction mills are
related to water consumption in regions where its supplies are limited. In oil
extraction systems, where the oil is extracted by either pressure or
centrifugation with a three-phase process (oil, liquid extract and residue)
found mostly in Italy and Greece environmental problems lie in both the large
volumes of water required and the removal of liquid extracts. To avoid damage
to the environment liquid extracts should be treated and puried before
discharge into water courses. Furthermore, the liquid extracts should be
applied to the soil, as liquid fertilizers. However, the quantities applied should
be low in order to avoid pollution of groundwater. In the two-phase process
signicantly less water is required, and this is the reason for its use in various
countries. Furthermore, with two-phase centrifugation a signicant amount of
residue is produced, requiring drying at high temperatures to extract the
remaining oil. Furthermore, the legacy of table olive production is a highly
polluting liquid waste. This waste has high organic and sodium contents,
rendering it unsuitable for application as fertilizer on olive groves.
295
296
Chapter 26
UTILIZATION OF OLIVE MILL BY-PRODUCTS

After the virgin olive oil has been extracted from olives, a liquid and solid residue
remains, consisting of the following: washing water, olive mill waste water
(OMWW), leaves and olive pomace. From these residues the pomace was
traditionally used in order to extract pomace oil, by solvent extraction; the
remainder of the liquid and solid residues were disposed of into the environment,
creating a pollution problem. However, in todays more enlightened world all
waste products require recycling, to both increase revenue and conserve energy.
The use of centrifugal decanters of either two or three phases results in the
production of olive oil with a higher content of antioxidants. Below, we analyse
the various by-products individually.
Washing water
The washing water used following olive processing attains a brown colour and
contains organic and inorganic materials that can cause pollution. Washing
water represents 10% (v/w) of the total weight of olive production waste.
Olive leaves
The leaves are collected, together with the fruit, and are transported to the
processing unit. The percentage of olive leaves is a function of the method used
for olive harvesting: a greater proportion from harvesting by the use of shakers.
A proportion of up to 58% is common. These leaves should be removed before
processing for oil extraction, since too many leaves give a very bitter taste to the
oil. The separated olive leaves could be used in the following ways: (i) after
drying, as a fuel in power plants, together with other plant material; (ii) as a
fertilizer, by incorporating them in the soil of the olive orchard, intact or
following crushing (leaves are a rich source of organic matter and mineral
nutrients); or (iii) for phenol production (12% of fresh weight). The most
important phenol is oleuropein, which gives olive oil its bitter taste and has
important properties for human health.
Olive pomace
Olive pomace is the nal product of the separation of olive oil, leaves, washing
water and OMWW. Its quantity and its chemical composition depend on the
type of decanter used for oil extraction, i.e. triphasic or biphasic. Olive pomace
has the following two uses: (i) as a fuel in power plants; and (ii) production of
Olive Mill Products
297
compost by mixing with other plant materials (Cegarra et al., 1996); this
compost mixture improves soil texture and nutrient composition.
Extraction of pomace oil with solvents

Table 26.1 presents the characteristics of olive pomace after processing by
three different techniques.
The endocarp is a fuel, since it produces only small amounts of ash and an
exhaust that is low in nitrogen gas (N2) and sulphurous gases. Therefore,
separation of stones from the wet olive pomace is a technique routinely used in
olive oil processing plants.
Preparation of compost from moist pomace

The moist pomace is mixed with other plant materials in order to supply
cellulose; it is then mixed with bovine manure in order to supply N; after a
period of 6 months a stable compost is produced. Furthermore, moist pomace
on its own could be used as a soil fertility additive.
Olive mill waste water (OMWW)

The OMWW is the liquid obtained after olive processing. It has a high content
of K, organic matter and phenols with antibacterial properties (Capasso et al.,
Table 26.1. Characteristics and quantities of olive pomace produced by three

different extraction methods: (i) traditional pressing; (ii) triphasic decanting; and (iii)
biphasic decanting (from Di Giovacchino and Preziuso, 2006).
Parameter
Total pomace (kg/t olives)
Moisture (%)
Oil (% f.w.)
Fibre (%)
Endocarp (%)
Ash (%)
N (mg/100g)
K (mg/100g)
P (mg/100g)
Total phenols (mg/100g)
Traditional
pressing
Triphasic
decanting
Biphasic
decanting
250350
2235
6.08.0
2035
3045
34
250350
4060
150200
200300
450550
4555
3.54.5
1525
2028
24
200300
3040
100150
200300
800850
6575
3.04.0
1015
1218
34
250350
4050
150250
400600
298
Chapter 26
1995). The quantity of OMWW is a function of the type of decanter used, i.e.
biphasic or triphasic. OMWW is a potential polluter, but it is also a source of
valuable materials such as oleuropein and hydroxytyrosol (Capasso et al.,
1999; Visioli et al., 2001; Allouche et al., 2004). This by-product can be
sterilized by various processes such as:
Chemical oxidation or Fentons reaction (Beltran-Heredia et al., 2001).

Photo-oxidation.
Electrolysis (Israilides et al., 1997).
Electrochemical oxidation.
Anaerobic degradation.
Aerobic degradation, by use of biolm or activated sludge.
Membrane technology, utilizing reverse osmosis or ultraltration. This
gives a liquid with low organic content and a more concentrated liquid or
sludge, with many organic compounds that can be separated and used.
Evaporation and distillation. Evaporation, involving formation of pools of
waste liquid, has been used in many countries (Di Giacomo et al., 1991).
Evaporation, however, is an expensive process in terms of thermal energy,
and in practice solar energy sometimes is adequate, especially in the
southern areas of Mediterranean countries.
The controlled spreading of OMWW on the soil of the olive orchard is a

common method for utilization of this liquid waste, since it achieves the
recycling of both organic and inorganic materials (see Table 26.2).
With regard to the numbers of microorganisms, after application of
OMWW their population increases but after 2 months decreases. These
microorganisms include nitrifying bacteria, Actinomycetes, yeasts, etc.
Table 26.2. Nutritional parameters of the effects of controlled spreading of

OMWW on the olive orchard (from Di Giovacchino and Preziuso, 2006).
OMWW
Parameter
Productivity (kg/tree)
Moisture of olive fruits (%)
Oil (%)
Free fatty acids (%)
Peroxide index (meq O2/kg)
K232
K270
Soil pH
Organic matter (%)
N (%)
Reducing substances (mg/100 g)
Control
5 l/m2
10 l/m2
30 l/m2
4.3
51.8
17.3
0.50
8.1
1.98
0.16
7.15
1.97
0.12
0.24
4.6
52.0
17.2
0.50
6.3
2.09
0.17
7.20
2.02
0.12
0.38
5.4
52.0
16.6
0.50
7.9
2.0
0.16
7.32
2.04
0.13
0.39
5.2
52.6
17.4
0.54
7.3
2.01
0.17
7.27
2.08
0.13
0.48
Olive Mill Products
299
ANTIOXIDANTS AND OTHER BIOLOGICAL BY-PRODUCTS

Olive mill waste water is the major by-product of olive oil production. At
present olive mills have to discard it, which increases the cost of disposal, contaminates soil and creates ecological problems. Olive mill waste water
contains hydroxytyrosol, which is amphiphilic and acts as an oilwater
interface; it also contains antioxidants (Visioli et al., 1995b; Allouche et al.,
2004). The ability of OMWW extracts to scavenge superoxide (Visioli et al.,
1999), as happens with hydroxytyrosol and oleuropein, suggests that
OMWW can be used in situations where Fenton and HaberWeiss reactions
take place. In such places we observe contaminant products of superoxide and
nitric oxide, which yield peroxynitrile, a powerful oxidant. Since foods very
often come in close contact with chlorine bleaches used as disinfectors, the
use of hypochlorous acid (HOCl) scavengers may provide additional
protection against reactive chlorine species. The major phenolic compounds
identied in OMWW extracts are listed in Table 26.3.
USE OF OLIVE MILL WASTE WATER AS FERTILIZER

Huge amounts of OMWW are produced in the process of olive oil extraction,
and these quantities represent a serious environmental problem. Therefore,
strenuous efforts are now being made to reduce pollution due to their
detrimental effect or to recycle them into valuable products.
Some of the techniques used include thermal concentration, treatment
with physicochemical methods or with microorganisms and their use directly
on agricultural soils in the form of liquid fertilizer (Tomati and Galli, 1992;
Ben Ruina et al., 1999; Di Giovacchino et al., 2005; Papadopoulos, 2006).
Application of OMWW, as a fertilizer, has many advantages for soil fertility, but
also several disadvantages and potential problems.
Table 26.3. Major phenolic compounds identied in OMWW extracts.

Phenolic compound
Concentration (g/100 g d.w.)
Hydroxytyrosol
Tyrosol
Elenolic acid
Oleuropein
Luteolin-7-glucoside
Quercetin
Cinnamic acid
Total phenols
1.56
0.85
4.3
0.5
0.22
0.13
0.55
8.4
300
Chapter 26
Advantages
Addition of extra water to the soil, especially given the low precipitation
levels and lack of irrigation water seen today in many olive-growing areas.
Improvement in the physical and chemical properties of the soil through
the addition of organic matter (OM) and enhancement of microbial
activity.
Contains large quantities of K, N, P and Mg (see Table 26.4).
Disadvantages
The presence of fatty acids and polyphenols, which reduce soil fertility.
It is difcult to store or dispose of the large amounts of OMWW, since it is
produced in a relatively short time, and particularly during a period of high
rainfall.
Direct application should be at a level no greater than 30 m3/ha/year for
OMWW from traditional olive mills.
Its application should be carried out at least 1 month before the time of
sowing of annual crops.
It should be applied at a minimum distance from trees.
Table 26.4. Characteristics of mature OMWW composts (d.w.) (from Cegarra et al.,
1996).
Parameter
pH (H2O)
Ec (1:10) (mS/cm)
Organic matter (%)
Organic carbon (%)
Nitrogen (%)
NO3-N (%)
C:N ratio
Phosphorus (%)
Potassium (%)
Iron (%)
Copper (mg/kg)
Manganese (mg/kg)
Zinc (mg/kg)
CEC (me/100 g)
SCO
MOS
7.84
7.66
56.43
29.37
3.11
0.36
9.44
0.87
2.61
0.50
52
241
245
109.8
8.73
5.03
37.40
18.78
1.44
t
13.04
0.22
3.25
1.30
62
268
73
96.2
SCO, sewage sludge and cotton waste mixtures watered with fresh OMWW; MOS, mixing of
11.1% of manure with 88.9% of OMWW sludge; t, traces.
Olive Mill Products
301
No data are available for the use of OMWW as compost for olive production.
Composting is probably a good method for recycling liquid wastes, leading to the
production of CO2, water, mineral salts and stabilized OM, which contains
humic-like substances. The process of composting is thermophilic and leads to
release of phytotoxins. Furthermore, OMWW may be mixed with plant waste
materials to be transformed into organic fertilizers or composts, which have no
phytotoxicity.
HYDROGEN PRODUCTION
Hydrogen is a clean energy source for both industrial and domestic
consumption. Biological production of hydrogen gas (H2) utilizes biophotolysis
of water and photo-fermentation of organic materials, usually carbohydrates,
by bacteria. Carbohydrate-containing solid wastes, such as olive mill waste,
can be used for hydrogen production by using suitable bio-process technologies
(Kapdan and Kargi, 2006). Utilization of such wastes for hydrogen production
provides inexpensive energy generation with simultaneous waste treatment.
Olive mill waste water can be used as a raw material for bio-hydrogen
production. It will require pretreatment to remove undesirable components.
The carbohydrate content is converted to organic acids, and subsequently to
H2, by the use of certain bio-processing technologies.
Glucose is an easily biodegradable carbon source. The bio-conversion of
1 mol of glucose yields, theoretically, 12 mol of H2 and conversion of 1 mol of
glucose to acetate yields 4 mol H2. However, when butyrate is the end product
only 2 mol H2/1 mol glucose is formed. In practice the yield of H2 does not
exceed 17 mol H2/1 mol glucose. In the case of cellulose, 2.18 mol H2/1 mol
glucose is produced when the concentration of cellulose in the waste is
12.5 g/l.
Fig. 26.1 is a schematic diagram showing the pathways involved in biohydrogen production from agricultural wastes containing cellulose and starch
by two-stage anaerobic dark photo-fermentation.
Agricultural wastes such as olive pomace contain starch and cellulose
(Kapdan and Kargi, 2006). Starch can be hydrolysed to glucose and maltose by
acid hydrolysis, and this is followed by conversion of carbohydrates to organic
acids and H2. Cellulose and hemicellulose content also can be hydrolysed to
carbohydrates, and subsequently to organic acids, which also produce H2.
Hydrogen is considered a viable alternative fuel of the future. Furthermore,
H2 is widely used in the production of chemicals, hydrogenation of fats and oils
in the food industry and desulphurization and reformulation of petrol in
reneries. Hydrogen has a high energy yield that is 2.75 times greater than
that of hydrocarbon fuels.
302
Chapter 26
Agricultural wastes
containing cellulose
OMWW
Grinding
Pretreatment
Pretreatment
Dark fermentation
Pyrolysis
Glucose syrup
Organic acids
H2
Photo-fermentation
Fig. 26.1. A schematic diagram showing the pathways involved in bio-hydrogen

production from agricultural wastes containing cellulose by two-stage anaerobic
dark photo-fermentation.
27
OLEUROPEIN, OLIVE LEAF EXTRACT,
OLIVE OIL AND THE BENEFITS
OF THE MEDITERRANEAN DIET
TO HUMAN HEALTH
OLEUROPEIN
Oleuropein as an antioxidant
Oleuropein is the bitter compound of olives that enhances nitric oxide production
and has free-radical scavenging properties (Visioli et al., 1998). This is commonly
found in the Mediterranean diet, which is rich in fresh fruits, vegetables and olive
oil and has been associated with a lower incidence of cardiovascular disease and
cancer. This is ascribed to its high proportion of vitamins, avonoids and
polyphenols (Ho et al., 1992). Oleuropein is the most abundant of the minor
components of olive oil; it has potent antioxidant and anti-inammatory
properties and enhances nitric oxide (NO) production in lipopolysaccharide
(LPS)-challenged mouse macrophages. The promotive effect is blocked by the
inductible nitric oxide synthase (iNOs) inhibitor N(G)-nitro-L-orginine methyl
ester (L-NAME). This indicates increased iNOs activity and that oleuropein
potentiates the macrophage-mediated response, leading to higher NO
production, which is considered to be benecial in cellular protection.
Antimicrobial activity of oleuropein and hydroxytyrosol

Secoiridoids, one of the major classes of polyphenols contained in olives and
olive oil, have recently been shown to inhibit or delay the rate of growth of a
range of bacteria and microfungi, but there are no data in the literature
concerning the possible involvement of these secoiridoids as agents against
pathogenic bacteria in man (Juven et al., 1968, 1972; Juven and Henis, 1970;
Ruiz-Barba et al., 1991; Aziz et al., 1998; Bisingnano et al., 1999). Oleuropein
303
304
Chapter 27
inactivates bacteria by dissolving their outer lining; the presence of 0.1% (w/v)
of oleuropein delayed the growth of Staphylococcus aureus. Intermediate concentrations (0.2%) of oleuropein prevented growth, while concentrations of
oleuropein > 0.2% inhibited growth and production of enterotoxin B in
articial nutrient media.
Caffeic and protocatechuic acids (0.3 mg/ml) inhibited the growth of E.
coli and Klebsiella pneumoniae. The same compounds, apart from syringic acid
(0.5 mg/ml), completely inhibited the growth of Bacillus cereus. Oleuropein and
p-hydroxy benzoic, vanillic and p-coummaric acids (0.4 mg/ml) completely
inhibited the growth of E. coli, K. pneumoniae and B. cereus. Vanillic and caffeic
acids (0.2 mg/ml) completely inhibited the growth of aatoxin production by
both Aspergillus avus and A. parasiticus. Olea europaea can therefore be
considered a potential source of promising antimicrobial agents for the
treatment of intestinal or respiratory tract infections in man.
Effect of oleuropein on the protection of low-density

lipoprotein (LDL) from oxidation
Oleuropein (105 M) effectively inhibited CuSO4-induced LDL oxidation (Visioli
and Galli, 1997). Polyphenolic components of the Mediterranean diet interfere
with biochemical events that are implicated in atherogenetic disease.
Therefore, there is a link between the Mediterranean diet and the prevention of
coronary heart disease. Oleuropein seems to both increase the high-density
lipoprotein (HDL) (Mensik and Katan, 1987) and lower the bad cholesterol
(LDL) levels by 30% or more without exercise or dietary changes (Baggio et al.,
1988; Aviram and Elias, 1993; Visioli and Galli, 1994; Visioli et al., 1995a).
Blood pressure-lowering effect of oleuropein and olive

leaf extract
Oleuropein has been tested for its blood pressure-lowering activity in
hypersensitive rats by daily oral doses of L-NAME 50 mg/kg) for at least
4 weeks. Oral administration of the leaf extract at different doses and
concurrently with L-NAME for 8 weeks reduced the rise in blood pressure at a
dose of 100 mg/kg. These conrm the hypotensive effects of oleuropein. This
antihypertensive effect may be related to various factors causing reversal of the
vascular changes produced during hypertension (Khayyal et al., 2002).
Benets of the Mediterranean Diet
305
Effect of oleuropein on inactivation of cytochrome P450

Oleuropein is an inhibitor of the CYP3A-mediated androstenedione 6hydroxylation in human hepatic microsomes. Furthermore, oleuropein is also
a potent mechanism-based inactivator of the CYP3A enzymes.
Oleuropein was clearly identied as a mechanism-based inhibitor, whereas
hydroxytyrosol was not. The possible mechanism of oleuropein inhibition is
associated with either its ester oxygen linkage or its non-terminal exocyclic
alkene bond. The inhibition of CYP3A by oleuropein may explain the in vivo
protective effects reported for oleuropein in LDL oxidation (Murray, 1999; Coni
et al., 2000).
OLIVE LEAF EXTRACT

Olive leaf extract (OLE) is a natural herbal antibacterial/antiviral extract
obtained from olive leaves, which is safe and non-toxic to humans (Cherif et al.,
1996; Walker, 1996a,b; Briante et al., 2002b; Khayyal et al., 2002; Lee-Huang
et al., 2003). The use of OLE started as early as 1800 when a tea made from
OLE was used for malaria, and in the early 1900s OLE was found to be far
superior to quinine in the treatment of malaria. In 1957, the active ingredient,
oleuropein, of OLE was isolated. Scientists reported that oleuropein could be
used effectively to lower blood pressure (Fehri et al., 1994), in the prevention of
intestinal spasms, the relief of arrhythmia and to increase blood ow to the
coronary arteries.
The product thus obtained is called Olivir, which is both virucidal and
bactericidal, manufactured from oleuropein and a blend of OLEs. Olive leaf
extract in a capsule form is a fairly recent advance, but in the early 1900s a
bitter compound was found in the leaves of certain olive trees, called
oleuropein, which is part of the olive trees disease resistance system. A Dutch
researcher found the chemical agent within oleuropein that could be medically
important, a chemical now known as elenolic acid. During the late
1960s/early 1970s a new multifunctional monoterpene was isolated from
various parts of the olive tree. This compound is calcium elenolate and is a
crystalline salt form of elenolic acid. It will act effectively at low concentrations,
without any harmful inuence on host cell mechanisms. When this compound
was injected directly into the bloodstream it was bound quickly to the blood
serum protein and became ineffective.
Calcium elenolate kills viruses by interfering with the process of
production of certain amino acids. This prevents virus assembly at the cell
membrane and therefore the penetration of infected host cells, and irreversibly
inhibits viral replication. In retroviruses, calcium elenolate neutralizes the
production of the reverse transcriptase (RT) and protease enzymes. These
enzymes in retroviruses (e.g. HIV) alter the RNA of healthy cells.
306
Chapter 27
Calcium elenolate in animals is not toxic, even in doses several hundred

times higher than those recommended. However, no studies have yet been
conducted concerning the use of OLE during pregnancy and the response to its
interaction with other human medicines.
Sometimes, symptoms such as fatigue, diarrhoea, headache, muscle/joint
aches and u-like symptoms may appear when using OLE. In such a case it is
recommended to discontinue taking the extract until the body can eliminate
the toxic substances accumulated.
Anti-HIV activity of olive leaf extract

Dried olive leaves (O. europaea) were rinsed in sterile distilled water to remove
dust, insecticides and contaminants. The leaves (1 g leaf/40 ml water) were
ground nely and extracted twice with sterile distilled water for 12 h at 80C;
centrifugation followed at 20,000 g for 30 min. The clear supernatant was
concentrated by lyophilization, reconstituted with water to 0.1 g starting
material/ml (1 g to 10 ml H2O) and sterilized by millipore ltration with a 0.45
m lter, and stored at 20C until use. Olive leaf extract has been used for
medicinal purposes for centuries, and substances contained in OLE have
activity against bacteria, mycobacteria and fungi. Furthermore, OLE lowers
blood pressure and inhibits lipid oxidation. The biological activity of OLE is due
mainly to polyphenolic compounds, especially oleuropein and hydroxytyrosol.
OLE is used in the treatment of HIV for various reasons, such as increasing the
tolerance of the immune system, relief of chronic fatigue and reduction of the
side effects of anti-HIV medication (Lee-Huang et al., 2003).
The mechanism of action of OLE in HIV is not known (Lee-Huang et al.,
2003). However, OLE increases the activity of the HIV-RT inhibitor 3TC. OLE
inhibits acute HIV-1 infection, cell-to-cell transmission and HIV-1 replication.
These effects occur at 0.2 g/ml OLE. Analysis of OLE indicates the presence of
three major compounds, oleuropein (MW 539), elenolic acid (MW 377) and
hydroxytyrosol (MW 153). Oleuropein accounts for 8590% of anti-HIV
activity. Treatment of HIV-1-infected cells with OLE up-regulates the expression
of the apoptosis inhibitor proteins. Treatment with OLE reverses many of the
changes induced by HIV-1 infection.
The polyphenolic compounds found in OLE are absorbed by the gastrointestinal tract and can be metabolized. Hence, oleuropein is metabolized to Delenolate and hydroxytyrosol to tyrosol. Therefore, OLE is both an anti-HIV
agent and it modulates the hosts response to infection. HIV-1 infection triggers
the following changes:
Modulation of the expression prole of cellular genes involved in survival,

stress, apoptosis, calcium and protein kinase C-signalling pathways.
Up-regulation of the expression of heat shock proteins.
307
DNA damage in inducible transcript-binding proteins.

Down-regulation of the expression of the anti-apoptotic BC12-associated
protein.
OLIVE OIL
Olive oil and human cancer
Greece, Italy and Spain obtain 71, 42 and 37%, respectively, of their total
dietary fat from olive oil. Olive oil and its minor constituents have benecial
effects on human health (Visioli and Galli, 1998a,b; Visioli et al., 2006).
Several epidemiological studies have evaluated the association between olive
oil consumption, human health and the occurrence of cancer (Visioli and
Galli, 1998a; Tripoli et al., 2005, 2006; Gaddi et al., 2006; Tripoli et al., 2006).
A minimum level of essential fatty acids (4% as linoleic acid) is required before
a saturated fat can display its full cancer-promoting potential. However, cancer
promotion depends not only on the amount but also on the fatty acid
composition of the fat (Cohen and Wydner, 1990). Olive oil-fed rats had high
levels of oleic acid in total serum lipids, as well as decreased tumour
prostaglandin levels. It was proposed that high-fat diets, rich in oleic acid, fail
to promote mammary tumorigenesis. This is due to competition with enzymes
in the linoleic acidarachidonic acid and prostaglandin-metabolizing enzyme.
Cancer development is associated with uncontrolled cell proliferation. For
signals of this proliferation, G proteins and the protein kinase C are involved;
these signals control the cell division cycle. Hence, lipid alterations might be
participating in the development of certain cancers; circumstantial evidence
for this lies in the fact that membrane lipids were altered in cell membranes of
patients suffering from cancer.
Several types of cancer cells show alterations in the membrane lipid layers,
and it is feasible to apply lipid therapy to treat certain cancers. Certain
anticancer drugs alter the lipid membrane structure; lipid therapy is relevant
to the development of anticancer drugs. Furthermore, high olive oil intake and
the use of Minerval, which is structurally and chemically close to oleic acid,
have been associated with a reduced risk of human cancer.
Breast cancer
The highest rates of breast cancer occur in the most industrialized regions of
the USA and Europe. Hence, the fat consumption per capita is highly correlated
with breast cancer and mortality (Howe et al., 1990; Trichopoulou et al., 1993;
Martin-Moreno et al., 1994; Fortes et al., 1995; La Vecchia et al., 1995;
Lipworth et al., 1997). For example, today, the incidence of breast cancer in
Spain is about 40% lower in comparison with the USA and northern Europe.
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Furthermore, Greek women, with 71% total fat intake from olive oil, have
signicantly lower mortality from breast cancer than women from the USA.
The Mediterranean countries offer an ideal testing ground for the role of
olive oil in breast cancer (Menendez et al., 2005), since olive oil in these
countries is the main oil used for cooking. The intake of olive oil is inversely
associated with the risk of breast cancer.
Ovarian cancer
In studies assessing changes in diet and cancer mortality in the Mediterranean
region, it was found that Greece and Spain had the lowest rates of ovarian
cancer and, at the same time, the lowest animal fat intake and the highest olive
oil intake. Ovarian cancer mortality is positively associated with total fat intake
in various countries (Lipworth et al., 1997).
The epidemiologic evidence concerning olive oil intake with respect to
ovarian cancer risk is limited, and there has been only one study conducted in
Greece. This study evaluated the association between ovarian cancer and
monounsaturated fat consumption, and indications from it are that there is an
inverse relation of monosaturated fat intake from olive oil and the risk of
ovarian cancer.
Colonic cancer
Studies in animals have indicated that certain fats in fatty acids may affect
colonic carcinogenesis (Bartoli et al., 2000; Stoneham et al., 2000), while olive
oil and sh oils have the lowest carcinogenic potential. The mechanism of
action is through cholesterol biosynthesis, which converts cholesterol to
primary bile acids. Many studies point to the relationship between monounsaturated fats and colonic cancer. Several studies on colorectal cancer have
been undertaken in Mediterranean countries (Bartoli et al., 2000; Stoneham et
al., 2000).
Endometrial cancer
In a hospital-based case-control study in women with conrmed cancer of the
endometrium, the only statistically suggestive association was an inverse one
with monounsaturated fat, which reduced the risk of cancer by 26% (Lipworth
et al., 1997).
Various studies indicate that olive oil may have a potential effect in
lowering the risk of this type of cancer. However, it should be established
whether the protective effect of olive oil derives from the monounsaturated
fatty acids of olive oil or whether it is related to its antioxidant properties.
Other cancers
An inverse relationship exists between olive oil consumption and various
cancers such as stomach, lung, bladder, urinary tract, oral cavity and
pharynx.
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Olive oil, membrane lipid composition and cardiovascular

diseases
Cardiovascular diseases constitute the major cause of death in developed
countries, and they are responsible for about 40% of all deaths. As a result of
alteration in the membrane lipid composition various cardiovascular disorders
have evolved, such as hypertension, atherosclerosis, coronary heart disease,
sudden cardiac death, blood vessel integrity, thrombosis, etc. The type of fats
consumed has been associated with cardiovascular health. Diet modies and
modulates the physical properties of membranes. Furthermore, membrane
lipid composition regulates localization and function of several membrane
proteins. One such case is the membrane concentration of various G proteins
(transducer signals from G protein-coupled receptors (GPCRs)) involved in the
control of blood pressure (Vigh et al., 2005). A relationship exists between
nitric oxide synthase (NOS) activity and membrane uidity during
hypertension. Protein kinase C and other protein kinases also participate in the
development of hypertension. One important function regulated by GPCRs is
the control of blood pressure. Signicant lipid alterations occur in cell
membranes of patients with cardiovascular disease. Therefore, lipid therapies
should be designed for treatment of high blood pressure.
Oleic acid is a free fatty acid that regulates GPCR-associated signalling.
The effect of oleic acid is exerted through its effect on proteinlipid interaction,
and not modication of the activity of pure G proteins. An oleic acid diet can be
considered as a form of lipid therapy. Experience shows that olive oil
consumption is associated with both reduced risk of development of
cardiovascular disease and reduced antihypertensive medication. Apart from
oleic acid, olive oil also contains tocopherol and phenols, which are believed to
exert a protective function (see below). Furthermore, daily inclusion in the diet
of 1 g omega-3 fatty acids reduces the likelihood of death due to cardiovascular
disease.
A compound analogous to oleic acid is the synthetic substance 2-hydroxy9-cis-octadecenoic acid (Minerval), which reduces blood pressure and
regulates G proteins in a similar way to oleic acid.
Olive oil and obesity

A high body mass index (BMI) is associated with increased incidence of
cardiovascular disease, diabetes and cancer. The BMI is determined as the ratio
weight:height2 and is expressed in kg/m2. A normal BMI ranges from 25 to
30 kg/m2, and anyone with a BMI over 30 kg/m2 is considered obese. Obesity
is associated with the accumulation of triacylglycerol, alteration in the
membrane lipid composition and variation in the physical properties of
membranes. Some of these changes are related to adrenergic receptors, which
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are involved in the control of body weight. Consumption of unsaturated fatty

acids (olive oil and sh oil) induces loss of weight and thereby improves health.
The use of clinical drugs designed to interact with proteins is usually
referred as chemotherapy. Therapies involving nucleic acids have developed in
recent years, and therapies for direct treatment of cells are known as cell
therapies. Another type of therapy is known as lipid therapy (see above), which
involves regulation of membrane lipid structure/composition.
Olive oil and diabetes

The use of olive oil activates insulin and controls the problem of diabetes. The
levels of heat stress proteins (Hsp) in diabetes are the result of reduced membrane
uidity. Due to oxidative stress and insulin deciency, diabetes and insulin
resistance are associated with stiffer and less uid membranes. Furthermore,
diabetes induces massive changes in specic lipid molecular species .
Olive oil and Alzheimers disease

This disease is a neurodegenerative disorder, characterized by the formation of
amyloid plaques and neurobrillary tangles in the brain. The pathology of this
disease may be the end result of abnormalities in lipid metabolism and
peroxidation. Antioxidants of olive oil and omega-3 lipids have shown benets in
reducing the effects of Alzheimers disease. Neural tissue has the highest lipid
content in the body, after adipose tissue. Neurodegeneration in Alzheimers disease
is accompanied by alterations in lipids, such as phosphatidylcholine hydrolysis.
Antioncogenic action of the oleic acid of olive oil

Dietary fatty acids can interact with the human genome by regulating the
amount and/or activity of transcription factors. This has opened up a whole
new line of research for the anticancer and antioxidant benets of the olive oilbased Mediterranean diet (Galli and Visioli, 1999; Benavente-Garcia et al.,
2000; Owen et al., 2000). Recent ndings reveal that oleic acid, the main
monounsaturated fatty acid in olive oil, can suppress the overexpression of
HER2 (erbB-2), a well-characterized oncogene, playing certain key roles in the
aetiology, progression and metastasis of several human cancers:
An exogenous supply of oleic acid signicantly down-regulates HER2coded neuroncoprotein in human cancer cells.
Exposure to oleic acid represses the transcriptional activity of the human
HER2 gene promoter in tumour-derived tissue.
311
Treatment with oleic acid induces the up-regulation of the Ets protein
PEA3 (a transcriptional repressor of HER2 gene amplication).
Treatment with oleic acid efciently blocks fatty acid synthase (human
gene) (FASN) activity.
Malonyl-coenzyme A (CoA) decreases HER2 promoter activity, while oleic
acid or CoA similarly up-regulate PEA3 gene promoter activity.
Antioncogenic and anticholesterol actions of the squalene of

olive oil
Squalene protects from cancer (Newmark, 1999) and atherosclerosis, since it
strongly inhibits the activity of 3-hydroxy-3-methylglutaryl-CoA reductase
(HMG-CoA reductase), a key enzyme in cholesterol synthesis. The HMG-CoA
reductase inhibitors are now effectively being employed as cholesterollowering drugs.
Effects of the phenolic compounds of olive oil

Anticancer potential
The Mediterranean diet, which is high in fruits, vegetables, bre, sh and olive
oil, is a healthy and disease-preventive diet. It has chemoprotective effects
against cancer and reduces heart disease problems and mortality percentages.
One signicant component of olive oil is oleic acid (C181), which is a
monounsaturated, long-chain fatty acid, while seed oils such as sunower are
rich in polyunsaturated linoleic acid (C182), which can more easily be
oxidized by various means.
Olive oil also contains minor components, which represent 1% of virgin
olive oil. These components, and especially phenols, have anticancer potential.
They number many hundreds and are classied within the following
categories:
Acids (phenoxy acids, triterpenic acids).

Phenols (tocopherols, epoxyphenols).
Chlorophyll.
Alcohols (mono- or triterpenic).
Aldehydes.
Esters (derivatives of fatty acids with alcohols).
These compounds contribute to olive avour, but some of them also give
bitterness or a burnt taste. The concentration (mg/kg) of individual phenolic
compounds in olive oil is: hydroxytyrosol, 14.42; tyrosol, 27.45; total simple
phenols (TSP), 41.87; total secoiridoids (TSID), 27.72; and lignans, 41.53.
The sum of TSP + TSID + lignans is 111.12.
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Inhibition of enzymatic activity

Most of the phenolics in olive oil are amphiphilic, and they partition between the
olive oil and waste water phases. The lipid-soluble antioxidants (tocopherols) do
not affect enzymes such as lipoxygenases, NADH oxidase or NOS. Hydroxytyrosol
inhibits platelet aggregation and the accumulation of the pro-aggregant agent
thromboxane. However, oleuropein increases the activity and the expression of
the inducible form of the enzyme NOS (iNOS). Nitric oxide inhibits platelet
aggregation. Furthermore, inhibition of nitric oxide synthesis increases cellular
damage and animal mortality.
Furthermore, olive oil phenols inhibit human hepatic microsomal activity.
Olive oil phenols tested positive as being inhibitors of human hepatic
microsomal androstenedione-6b-hydroxylation and reductive oxidative 17hydroxysteroid dehydrogenase (17-HsB) activity.
Inhibitory action on reactive oxygen species
Seed oils are devoid of the typical phenolic compounds present in olive oil. The
lignans (+)-1-acetoxypinoresinol and (+)-pinoresinol are major components of
the olive oil phenolics. The fraction (+)-pinoresinol is present in the seeds of
Sesamum indicum and (+)-1-acetoxypinoresinol is present in the bark of
O. europaea. If we compare virgin olive oil and rened virgin olive oil we nd
that the sum of hydroxytyrosol and tyrosol is ten times greater in the former.
The effect of olive oil on the enzyme xanthine oxidase, as measured by the
production of uric acid, indicates that the minimum amount of uric acid (M)
was produced with the extra virgin olive oil, intermediate with the rened
virgin olive oil and the maximum with seed oils. The amount of uric acid
produced with seed oils is four times greater in comparison with extra virgin
olive oil and two times greater in comparison with rened virgin olive oils. The
effect of total phenolic compounds within olive oil on xanthine oxidase activity,
which is measured by the production of uric acid (M), indicates that the
greater the total phenol level (g/ml), the less uric acid is produced. Total
phenols over 400 g/ml reduce uric acid production three- to 15-fold. The
inhibition of xanthine oxidase activity by extracts of oils indicated that
100500 l methanolic extract of extra virgin olive oil could reduce the
production of ROS four- to vefold, while with rened olive oil or seed oil the
production of ROS was at its maximum.
Table 27.1 lists the inhibitory concentration of various phenolic compounds
on the production of ROS that attack salicylic acid in an assay of hypoxanthine/
xanthine oxidase.
Methanolic extracts of olive oils have signicantly greater antioxidant
capacities in comparison with seed oils, as measured by the hydroxylation of
salicylic acid by HO. Antioxidants interfere with the hydroxylation of salicylic
acid by donating a proton to HO. Inhibition of xanthine oxidase activity will
reduce the amount of superoxide generated, and consequently the production
of HO by Fentons reaction. Total phenols inhibit directly HO attack on
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Table 27.1. Inhibitory concentration of phenolic compounds of olive oil on reactive

oxygen species that attack salicylic acid in an assay of hypoxanthine/xanthine
oxidase (from Owen et al., 2000).
Phenolic compound
Hydroxytyrosol
3,4-Dihydroxybenzoic acid
Tyrosol
p-Hydroxybenzoic acid
Vanillic acid
Caffeic acid
Syringic acid
p-Coumaric acid
Ferulic acid
o-Coumaric acid
Cinnamic acid
Benzoic acid
Catechol
Oleuropein glucoside
Trolox
Dimethylsulphoxide
Inhibitory concentration (mM)

1.34
3.03
2.51
1.69
2.70
6.05
3.19
2.33
1.56
2.32
2.33
2.75
2.50
7.80
12.24
2.30
salicylic acid and indirectly via inhibition of xanthine oxidase activity.

Hydroxytyrosol and tyrosol act as antioxidants via proton donation, while
lignans of olives have a dual action, i.e. proton donation to HO and inhibition
of xanthine oxidase. Therefore, the major phenolics of olive oil have signicant
dietary value and, moreover, they increase the defence against ROS and
decrease the activity of xanthine oxidase, which is one of the factors
responsible for carcinogenesis. The high concentration of lignans in olive oils
has been shown to inhibit skin, breast, colonic and lung cancer. The
mechanism of lignin inhibition of carcinogenesis is based on its antiviral
and antioxidant activities, and also in part on its anti-oestrogenic effects.
Lignans have been shown to inhibit oestradiol-induced human breast
carcinoma cells.
Protection of human erythrocytes from oxidative damage
Polyphenols are widely distributed in the vegetable kingdom and are present in
high concentrations in the Mediterranean diet, as components of olive oil.
However, the benets of olive oil consumption on human health can be
ascribed not only to the elevated oleic acid content but also to the antioxidant
properties of its minor components, including polyphenols and especially
hydroxytyrosol (3,4-dihydroxyphenyl ethanol), which is present in high
concentrations in extra virgin olive oil (free or esteried), accounting
for 7080% of total polyphenols. Hydroxytyrosol is an efcient scavenger
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of peroxyl radicals and is responsible for protection of the oil against the
auto-oxidation of unsaturated fatty acids. Furthermore, it was proved to act as
a powerful inhibitor of peroxidation of human lipoproteins by in vitro studies.
Hydroxytyrosol counteracts free radical-induced cytotoxicity in human
intestinal epithelial cells in culture.
The red blood cells (RBCs) are exposed to oxidative hazards due to their
specic role as oxygen carriers. Therefore, auto-oxidation of haemoglobin (Hb)
produces anion superoxide radicals, which are transformed to hydrogen
peroxide (H2O2). These compounds, when Fe ions are present, form the highly
reactive hydroxytyrosol radical. These radicals damage both plasma membrane
and cytosolic components, leading to oxidative haemolysis.
A balance exists between the production of ROS and their destruction by the
endogenous defence system (enzymes and vitamin E). When no such balance
exists (oxidative stress) it leads to chronic oxidative stress, as in the case of
hereditary anaemias, which can be ascribed to either an impaired antioxidant
defence system or overproduction of ROS (-thalassaemia). In human RBCs, the
molecular target of H2O2 is Hb, which is converted to the oxidized forms of Hb
and ferryl Hb. Other alterations of RBCs by H2O2 include side-chain protein
alterations and lipoperoxidation. These oxidative modications lead to changes
in the shape of the RBC and to haemolysis.
Even a small increase in phospholipid hydroperoxides in membranes
produces marked changes in the molecular organization of the lipid bi-layer.
The well-known oxidative stress-induced reduction in cellular energy charge
affects the RBC transport system in a different way. Due to oxidation, stress
methionine transport decreases. This amino acid, which is not utilized in
protein biosynthesis, is converted to an s-adenosylmethionine (SAMe) donor.
This substance is the key intermediate by which methionine is converted to
cysteine, which is a precursor of glutathione.
Inhibitory action on reactive nitrogen species

The concentration of phenols in olive oil varies from 100 to 800 mg/kg. This
concentration is a function of species, location, climate, stage of maturation
and the conditions of processing and storage of olive oil.
Phenolics from virgin olive oil are also powerful scavengers of superoxide
anions and hydrogen peroxide, and prevent both the generation of ROS and the
aggregation of platelets, which result in disorders of the cardiovascular system.
Besides the ROS there are also the reactive nitrogen species, such as the
radical nitric oxide (NO), which is involved in cancer and other diseases when
in excess concentration (De la Puerta et al., 2001).
This NO toxicity involves a fast reaction with superoxide radical (O2),
producing peroxynitrite (ONOO). This radical induces the peroxidation of
lipids, oxidizes methionine and S-H into proteins and causes DNA damage.
315
Hydroxytyrosol possesses a highly protective action against this radical,

leading to the nitration of tyrosine and DNA damage. Nitric oxide is also a
messenger molecule in neurotransmission. Oleuropein is able to scavenge
nitric oxide, since it reduces the amount of nitrite formed by the reaction
between oxygen and nitric oxide, produced from nitroprusside. Furthermore,
most olive oil phenolics are able to scavenge ONOO, except tyrosol, which is
less active. Therefore, components of olive oil contribute to protection against
oxidative processes and damage by reactive NO-derived nitrogen species
involved in several chronic diseases. Furthermore, oleuropein has proved
signicantly more toxic to Gram-positive than to Gram-negative bacteria. The
o-diphenol system present on its backbone structure is very likely responsible
for the antimycoplasmal activity of oleuropein. Oleuropein was also found to be
effective against Mycoplasma fermentans strains, which are naturally resistant
to erythromycin and to tetracyclines.
Antimycoplasmal activity
Among olive oil phenols, oleuropein has been shown to inhibit or delay the rate of
growth of mycoplasmas (Furneri et al., 2002), and a range of bacteria and fungi.
Furthermore, the major phenolic compounds of green olives have shown various
antimicrobial properties, such as the inhibition by oleuropein of Lactobacillus
plantarum, Pseudomonas fragi, Staphylococcus carnosus, Enterococcus faecalis,
Bacillus cereus, Salmonella spp. and fungi. Oleuropein also has antimicrobial
activity against Gram-positive and Gram-negative bacterial strains (Salmonella
spp., Vibrio spp. and Staphylococcus aureus).
The antimicrobial action of phenols is related to their ability to denature
proteins, generally by causing leakage of cytoplasmic constituents like protein,
glutamate or potassium and phosphate from bacteria, possibly due to
disruption or damage to the cell membrane. Oleuropein affects a signicant
leakage of glutamate, potassium and inorganic phosphate from E. coli, has no
effect on the rate of glycolysis and causes a decrease in the ATP content of cells.
Phenolic extracts from olive mill waste water and their
hypocholesterolaemic effects
Dietary phenolic compounds recovered from OMWW have antioxidative effects
and are very potent in high cholesterol-fed rats. The concentration of
hydroxytyrosol in OMWW is 100- to 500-fold higher than that in olive oil. The
overabundance of free radicals in cells causes chain reactions and lipid
peroxidation, processes linked with the development of both atherosclerosis
and cancer.
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Chapter 27
THE MEDITERRANEAN DIET

Introduction
The Mediterranean diet is rich in virgin olive oil as a source of fat, and in
vegetables, fruits, legumes and other plant foods, and low in saturated and
trans-fatty acids and cholesterol. The Mediterranean diet is associated with
longevity and low cardiovascular risk (Dominguez and Barbagallo, 2006). A
relationship between diet and cardiovascular health was shown scientically
many decades ago. There are many reports in the literature concerning the
protective role of the Mediterranean diet in human health (Ferro-Luzzi and
Sette, 1989; Ferro-Luzzi and Ghiselli, 1993; Landa et al., 1994; Keys, 1995;
Haber, 1997; Mancini and Rubba, 2000; Trichopoulou et al., 2000; Alarcon de
la Lastra et al., 2001; Wahrburg et al., 2002). However, by the middle of the
20th century, due to industrialization and dietary changes, the need to establish
preventive measures against the ill effects of poor diet became more pressing.
Diets rich in saturated fats are associated with cholesterol accumulation and the
risk of coronary heart disease. The Mediterranean countries, which in their diet
consume considerable amounts of virgin olive oil, are characterized by a low
level of heart attacks. This cardiovascular protection is achieved by the effects of
virgin olive oil on plasma lipid risk factors and on other factors such as blood
pressure and insulin sensitivity. The Mediterranean diet provides additional
benets by acting on other cardiovascular risk factors, including the reduction
of blood pressure in individuals with normal or high blood pressure and the
improvement of carbohydrate metabolism, not only in healthy people but also in
patients with the type 2 diabetes.
Low-density lipoproteins are protected from oxidative damage in individuals
consuming much virgin olive oil, in comparison with those consuming
polyunsaturated fatty acid-enriched diets. Furthermore, the Mediterranean diet
creates a less prothrombotic environment by modication of platelet aggregation
and total plasma factor VII.
The Mediterranean diet and ageing

A great number of people in developed countries reach an advanced age, but
with concomitant diseases such as Alzheimers, Parkinsons, vascular
dementia, cancer and diabetes. A diet rich in olive oil, high in monounsaturated
fats, is associated with a reduced risk from age-related diseases. Furthermore,
mitochondrial membranes are very sensitive to free radical attack, due to the
presence of a double carboncarbon bond in the lipid tails of their phospholipids,
and unsaturated (oleic) fatty acids decrease cellular oxidative stress. Virgin olive
oil strengthens membranes by increasing their resistance to free radicalinduced modications. Hence, virgin olive oil preserves mitochondrial function
317
and its electron transport chain, with both DNA and blood better protected
against oxidation.
Minor components of the Mediterranean diet

The Mediterranean diet uses mostly olive oil, which contains monounsaturated
fatty acids and several minor components with biological activity, as opposed to
seed or other oils. One such component is squalene (see above), which is, in part,
responsible for the low incidence of cancer seen in the Mediterranean countries.
The quantities of -tocopherol (vitamin E) and carotenoids are low; however,
these constituents exert an antioxidant role in the human body. The sterol acyl
coenzyme A, an inhibitor of acyltransferase activity, leads to lower levels of
plasma LDL cholesterol. Oleanolic acid also has antioxidant properties. The
most important phenolic compounds contained in virgin olive oil, and therefore
in the Mediterranean diet, are tyrosol, hydroxytyrosol and oleuropein, which
are absorbed by the human digestive system. These components have both
antioxidant and chemoprotective activity and a role in endothelial function
improvement. Furthermore, these compounds can modify haemostasis, inhibit
platelet aggregation and exert antithrombotic activity. Phenolic compounds
from the Mediterranean diet delay atherosclerosis.
The Mediterranean diet and cancer prevention

The diet and fatty acid intake are important factors associated with
development of cancer especially gastrointestinal, breast and prostatic. In
Mediterranean countries, where virgin olive oil is the main source of fat, the
incidence of cancer is lower in comparison with that in northern European
countries or the USA. The main mechanisms involved in protection from
cancer are the following:
Prevention of DNA oxidative damage.

Prevention of changes to cell membranes.
Prevention of gene expression modulation, which leads to DNA damage.
Avoidance of an altered expression pattern of new cancer genes (oncogene
HER2).
28
BIOLOGICAL AND INTEGRATED
OLIVE CULTURE
BIOLOGICAL OLIVE CULTURE

Biological (or organic) olive culture is an agricultural management system that
reduces signicantly the use of chemical fertilizers and phytoprotective materials.
Therefore, biological culture means food production that is not harmful to the
environment with regard to the use of fertilizer and phytoprotective chemicals.
Organic olive cultivation is often considered to be superior to conventional
and other alternative forms of agriculture and to be of greater value to society
(Lampkin and Padel, 1994; Conacher and Conacher, 1998; Fabbri and
Ganino, 2002; Parra-Lpez and Calatrava-Requena, 2005). The regulations
surrounding organic olive cultivation with regard to techniques used cover
three categories: obligatory, recommended and forbidden. The two most
important aspects are the following:
The initial planning of the biological olive orchard.

Provision of pruning, irrigation and pest control.
Biological pest protection achieves ecological stability by maintaining the

pathogen population at a level that does not cause signicant economic loss.
Pest and disease control may be performed in the following ways:
Selection of an olive variety tolerant to pests and diseases.

Use of appropriate propagation material and optimum time of planting.
Maintenance of optimum conditions for olive tree growth.
Balanced fertilization and use of green composts.
Destruction of certain species of plants that provide shelter for harmful
insects and diseases.
Soil fumigation and disinfection of propagation material.
With regard to those chemicals used in pest and disease control, for
biological olive culture the use of the following techniques only is permitted:
Use of various traps (pheromone or colour traps).
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Biological ways of dealing with pests and diseases, e.g.: (i) optimizing the
performance of Bacillus thuringiensis endotoxins; (ii) enhancing benecial
organisms that are parasites of Dacus or Prays oleae, such as Opius concolor,
Eupelmus urozonus, Chrysopus, etc.; (iii) using companion plants and
botanical extracts as alternative pest controls.
Use of selected insects that are parasites of those insects that cause damage
to the olive tree or fruit.
The following phytoprotective materials are permitted in biological olive

culture:
Beeswax for protection of large sections of the orchard during pruning.

Neem (Azadirachta indica) extract used as an insecticide.
Zelatin used as an insecticide.
Vegetable oils used as insecticide, acaricide and/or fungicide.
Rotenone (Derris spp. and Lonchocarpus spp.) and pyrethrin (Chrysanthemum
cinerariaefolium) used as insecticides.
Copper in the form of hydroxide or sulphate used as fungicide. The
permitted quantity is 0.6 kg/1000 m2 of olive orchard.
Calcium polysulphate used as fungicide, insecticide or acaricide.
Parafn oil used as insecticide or acaricide.
Potassium permanganate (KMnO4) used as fungicide or bactericide.
Sulphur used as fungicide or acaricide.
Various traps.
PRODUCTS PERMITTED FOR USE IN BIOLOGICALLY

CULTIVATED OLIVE ORCHARDS
Fertilizers
The following materials are permitted for use: the manure of agricultural
animals, dried chicken manure, composts from animal manure, animal
excretions, peat, perlite, vermiculite, mixtures of vegetative materials, animal
by-products (sh, meat or bone our), wood ash, milled phosphate rocks, K
salts such as sylvinite, potassium sulphate, CaCO3 and gypsum.
Phytoprotective materials
The following are permitted for use: Azadirachtin, which is produced from
Azadirachta indica (the neem tree), beeswax, zelatin, hydrolyzed proteins,
lecithin, water extract of Nicotiana tabacum, vegetable oils (pine, mentha, etc.),
pyrethrins produced from the plant Chrysanthemum cinerariaefolium, extracts
Biological and Integrated Olive Culture
321
from Quassia amara (a South American shrub) and rotenone from Derris spp.
and Lonchocarpus spp.
Microorganisms
Various microorganisms, such as Bacillus thuringiensis, granulosis virus, etc.,
are used in the control of dangerous and damaging organisms
Substances used in traps

The substances used in biological traps include metaldehyde, pheromones,
pyrethrins and ammonium phosphate.
Other materials
Copper (hydroxide, sulphate, etc.), ethylene, soap, parafn oil, KMnO4, SiO2, S
and calcium polysulphate can also be used in biological olive culture.
Organic olive growing has expanded in recent decades in Italy, Spain and
Greece. This can be explained partly by the health-giving properties of virgin
olive oil, which results in high prices for the grower. Of course, although
organic olive growing results in lower production and/or higher cost, due to its
higher quality, people prefer to consume organically produced olive oil.
Organic olive growing in Greece started ofcially in 1993 with a few hectares;
by 1998 it represented 10,000 ha and, by 2001, 15,500 ha.
TECHNIQUES OF CULTIVATION IN ORGANIC

OLIVE GROWING
Soil cultivation and fertilization
Every 2 years, in autumn, vetch is sown plus a cereal such as barley, for green
compost. Also during autumn, compost or manure (ovine or caprine) is added,
at 2025 l/tree, depending on its age and size. Composts containing K and
micronutrients, such as B, may also be used at that time.
Cover crops
Cover crops play a vital role in organic olive cultivation (Pardini et al., 2002,
2003). Use of cover crops is necessary in order to control weeds and soil erosion
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and to improve the quality of the olive oil. Furthermore, cover crops increase
the content of the organic matter of soil and improve overall soil fertility,
nutrient availability, soil structure and soil microorganisms. Cover crops also
reduce the pest and disease problem, reduce nutrient leaching and supply food
for livestock. When cover crops include legume species, the N content of the soil
is increased. There is a disadvantage, however, in the competition created with
olives and a concomitant decrease in olive yields.
Weed control
Winter weeds are controlled by soil cultivation during spring or by cutting
(milling cutter). In steep orchards the use of machinery is difcult and it is
recommended to use a hedge trimmer and leave the cut weeds on the soil surface.
Irrigation
In at areas, where water is available, drip irrigation is a method that conserves
water and reduces the cost of irrigation.
Pest and disease control
Olive y (Bactrocera oleae). Various traps can be used, such as ecotrap traps,
Delta traps of bait smeared with glue and bottles of different colours and
shapes lled with water and bait.
Mediterranean black scale (Saissetia oleae). Appropriate pruning achieves
an adequate population reduction in this insect. Furthermore, sprays with
Cu or oils are helpful.
Olive moth (Prays oleae). Use of pheromone traps, Bacillus thuringiensis at
the initiation of owering, wettable sulphur and control of irrigation are all
helpful measures.
Peacock spot (Cycloconium oleaginum). Correct pruning (open centre) and
spraying with Cu products are recommended.
Olive knot (Pseudomonas savastanoi). Spraying with Cu immediately after
pruning is recommended.
Harvesting
In biological olive cultivation table olives are harvested by hand. The olives of
the cultivars destined for olive oil production drop spontaneously onto plastic
nets or they are harvested by using shakers.
Biological and Integrated Olive Culture
323
Olive oil extraction

The olive fruits are immediately transported, on the same day, to the olive oil
processing unit. The temperature of the water for oil extraction should be
<30C, the oil thus maintaining all its aromatic substances. The extracted
olive oil is stored in stainless steel containers.
APPLICATION OF THE INTEGRATED MANAGEMENT OF

OLIVE ORCHARDS
The prevailing socio-economic conditions, the very fast technological progress,
the increase in world population, the progress in ecological aspects and the
increased demands of consumers for high-quality products have resulted in the
development of new agricultural management systems, such as the conventional,
organic or biological and integrated olive culture.
Conventional or chemical olive culture, representing a high proportion on a
worldwide basis, in its attempts to increase productivity and ease global food
shortages, has damaged the ecosystem (Parra-Lpez and Calatrava-Requena,
2006): productivity increases but natural resources and biodiversity are
consequently reduced. Without underestimating its role in the Green revolution,
this form of olive culture may create a series of unpleasant consequences.
Ever-bearing olive culture is a new form of agriculture that tries to achieve
adequate productivity but at the same time with minimum cost and with
respect for the natural environment. Ever-bearing olive culture may be
described as either: (i) ecological, biological or organic olive culture (Cobb et al.,
1999); or (ii) integrated or balanced olive culture (Pacini et al., 2003).
The main targets of this type of olive culture are the following:
Development of methods of cultivation that respect the natural environment

and are economically and practically feasible.
Maintenance of the ever-bearing feature.
Improvement of product quality.
Protection of growers health during the process of production, the
production of high-quality olive oil and improved health benets for olive
oil consumers.
Maintenance of biodiversity.
Use of alternative means of dealing with pests, diseases and weeds to the
use of chemicals.
Maintenance of soil fertility.
Certain conditions should be fullled in order for integrated olive management to be feasible:
Adequate research of market requirements.

Development of the necessary technology.
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Chapter 28
Development of methods for quality control of the product.

Practical and theoretical training of growers.
Selection of certain areas for olive culture on the basis of soil and climatic
conditions. Frost- and wind-affected areas and soils with bad drainage
should be avoided for olive culture.
Choice of the best planting system and varieties.
Soil cultivation and rational irrigation and fertilization. For integrated
olive oil production soil fertility, mineral nutrients, organic material in the
soil and normal soil microorganisms should all be maintained. Fertilizers
should be added only when there is a need, based on soil and leaf analysis.
Water availability is a prerequisite for integrated olive culture; however,
high soil moisture can promote the development of diseases.
Choice of the most appropriate pruning system.
Integrated pest and disease management (use of biological and
biotechnological methods). In order to maintain the ecological stability
and the natural biodiversity of an olive orchard it is recommended to
reduce the use of herbicides and also to maintain weed zones; such zones
constitute shelter for certain organisms.
Care in maintaining the quality of the product during harvesting,
transport to the processing units and the correct storage of both oil and
table olives. Olives are harvested when the fruit reaches its peak content
and quality of olive oil. During the transport of olives, fruit bruising should
be avoided, since oil quality quickly deteriorates. After harvesting, olives
should be processed very quickly in order to avoid deterioration and to
increase oil acidity. Furthermore, the extracted oil should be stored
appropriately to avoid oxidation.
29
CHEMICAL AND INTEGRATED WEED
MANAGEMENT IN OLIVE ORCHARDS
CHEMICAL WEED MANAGEMENT

Weed control in olive orchards is necessary, since if not correctly managed
weeds can compete with olive trees for water, nutrients and sunlight, and can
reduce olive tree productivity. The weeds present in olive orchards may be
annual, biennial or perennial plants. Such plants complete their life cycle from
a few months to 3 years. Weed management is a fundamental agricultural
practice, since existing weeds can inuence the population of insects, mites,
nematodes and diseases. Weed control should start before planting of olive
trees, since their control later is not easy. Furthermore, when the weeds
become dry there is the danger of re and destruction of the olive grove
(UCIPM, 2001).
Herbicides are valuable when applied to both deciduous and evergreen
trees. Herbicides can be classied as either pre-emergence or post-emergence.
Another classication system is in-contact herbicides and herbicides with
hormonal action. The toxicity of hormonal herbicides is closely related to the
vegetative activity of the plant where the herbicide has been applied. In order
for weeds to be killed, the products of photosynthesis after spraying of a
herbicide should be transported to the root system. When perennial weeds are
exposed to water stress conditions, an insignicant quantity of herbicide is
transported to the root system, causing necrosis of the weed. This is not so
critical in annual weeds, since only the parts above ground should die.
Hormonal herbicides are employed to control weeds without damaging
the main crop. Some selective herbicides are applied to leaves and some on the
soil. After spraying of the foliage the herbicide is transported from leaves to
young shoots or from older leaves to roots. This transport system follows the
translocation of carbohydrates via the phloem. The herbicides selectivity is
affected by leaf morphology, the degree of herbicide absorption and transport
and activation of certain enzymes.
325
326
Chapter 29
Herbicide absorption
Herbicides are usually absorbed by the roots or leaves. The amount of herbicide
absorption depends on the vegetative stage of the weed, since the leaf cuticle is
thicker in old leaves in comparison with young ones. The number and size of
stomata also affects herbicide entry into the leaf. Weeds differ in both the
number of stomata/mm2 of leaf surface and the location of stomata (on the
upper or lower leaf surface).
The efcient use of herbicides depends on their ability to pass through the
cuticle and plasmalemma. The entrance of herbicides is via stomata, if they are
open during the time of herbicide application. Application of herbicides to
green and tender shoots of weeds is frequently as effective as when applied to
the leaves. The bark of woody shoots is usually the obstacle to entrance of
herbicides. However, in some barks there are openings that allow the herbicide
ingress. Lenticels may also constitute a route for herbicidal entrance.
The most dangerous weeds causing losses to olive culture are Cynodon
dactylon, Oxalis and Rubus fruticosus. Other weeds present in olive orchards
include Sorghum halepense, Convolvulus arvensis, Cirsium arvense, and Amaranthus
(see Table 29.1).
WEED MANAGEMENT
Weed management varies from orchard to orchard and, of course, upon the
weed species present in the orchard, the soil characteristics, the irrigation
system and the age of the orchard.
Soil properties
Soil texture and organic matter determine the weed species present in the
orchard, the activity and adsorption of herbicides, the number of times soil
should be cultivated and when planting should take place. In sandy soils less
herbicide is used, and these soils require more cultivation to deter weeds. On
the contrary, clay soils are cultivated less frequently and can adsorb a greater
quantity of herbicide. Soil characteristics, where herbicides are applied, affect
their runoff. Soil texture and organic matter content affect the speed of
inltration and runoff, which are greater in sandy soils (Galindo et al., 2006).
Organic matter affects herbicide mobility and adsorption: soil compaction
decreases the speed of herbicide inltration and increases herbicide
concentration in the water ow. Stability of soil aggregates affects the rate of
inltration, as does the slope of the orchard, since a greater angle of incline
increases runoff.
Chemical and Integrated Weed Management
327
Table 29.1. Common and scientic names of weeds found regularly in the olive grove.
Common name
Scientic name
Asparagus
Barley
Bermuda grass
Bindweed
Blackberry
Bluegrass
Brome grass
Canary grass
Chickweed
Clover
Cocklebur
Dandelion
Dock
Fescue
Foxtail
Goosefoot
Johnson grass
Mallow (cheeseweed)
Mustard
Nettle
Nightshade
Oat
Pigweed
Purslane
Star thistle
Thistle
Asparagus ofcinalis
Hordeum murinum subsp. leporinum
Cynodon dactylon
Convolvulus arvensis
Rubus spp.
Poa annua
Bromus spp.
Phalaris canariensis
Stellaria media
Trifolium or Medicago spp.
Xanthium spp.
Taraxacum ofcinalis
Rumex crispus
Festuca rubra
Setaria spp.
Chenopodium murale
Sorghum halepense
Malva parviora
Brassica spp.
Urtica spp.
Solanum spp.
Avena fatua
Amaranthus spp.
Portulaca oleracea
Centaurea salstitialis
Salsola fragus
Irrigation
Total precipitation, its distribution and the method of irrigation all affect the
frequency of cultivation and the choice of herbicides. The amount of water
required following herbicide application is 1025 mm, and large amounts of
irrigation water result in herbicide movement into the soil below the tree
canopy. Therefore, the efciency of the herbicide is reduced.
Age of the orchard

We can distinguish three periods during the lifespan of the olive orchard at
which application of herbicides is necessary: (i) before planting; (ii) the young
olive orchard; and (iii) orchard 4 years old or over.
328
Chapter 29
Before planting
It is ideal to start weed control before planting of the orchard. In that way weed
competition during planting will be reduced. The methods available to control
weeds before planting include the following:
1. Digging and irrigation to prevent germination of weeds. Afterwards, it is
necessary to dig again in order to kill any remaining weeds. This method
controls mostly annual weeds.
2. Summer digging, when the soil is dry. Thereby, the rhizomes of perennial
weeds are divided into small parts and, with sunshine, they desiccate and die.
3. Soil solarization. The moist soil is covered with a plastic sheet during the
summer. The radiant energy trapped in the soil increases soil temperature,
killing various pathogens and weed seeds.
4. Use of pre-emergence or post-emergence herbicides before planting. Postemergence herbicides have some residual effect on the soil and are better
applied before planting.
Young olive orchards
In young orchards both chemical and non-chemical methods are available:
1. Hand-weeding around trees and destroying weeds between rows by
discing.
2. Use of plastic mulch around young trees.
3. Application of pre-emergence and post-emergence herbicides in one of three
ways: (i) a circle around trees; (ii) in zones along the tree row; and (iii) total
coverage of the orchard oor with herbicides. More than one herbicide is needed
in order to control the various species of weeds. It is worth noting that preemergence herbicides do not tackle existing perennial weeds in the orchard.
4. Cover crops also can decrease weed population in the area between tree
rows; these should not compete with olive trees. Some of the plants appropriate
as cover crops include the autumn-sown cereals, subterranean clover and
Bermuda grass.
Olive orchards 4 years old and older
When olive trees are established, weeds are not as competitive as before.
However, perennial weeds can still reduce fruit yield. These weeds may be
controlled in the following ways:
1. Destruction of weeds between tree rows by agricultural machinery, i.e. by
mowing or discing. However, cultivation may damage the roots of olive trees
and increase the danger of pathogenic infection.
2. Use of mulches to reduce weed growth.
3. Control of very young weeds between rows by aming. This method
controls the broadleaf weeds by destroying with intense and localized heat.
329
4. Lastly, pre-emergence and post-emergence herbicides can be used. Sometimes,

more than one application of herbicide is required in order to eliminate the
problem.
TYPES OF HERBICIDES USED AND MODES OF ACTION

Herbicides are divided into pre-emergence and post-emergence (Elmore, 1994).
Pre-emergence
These herbicides are sprayed on the soil and inhibit germination of weed seeds.
The herbicide should enter the soil at a depth of 38 cm, through the effects of
either rain or irrigation. Pre-emergence herbicides include Diuron (Karmex),
simazine (Princep), oryzalin (Suran), oxyuorfen (Goal) and napropamide
(Devrinol). Pre-emergence herbicides maintain their effectiveness from several
weeks to 1 year.
Table 29.2 presents some pre-emergence herbicides used in olive orchards,
and their rates of application.
Post-emergence
These herbicides are foliarly applied to either young or perennial weeds, and
they can be divided into two categories: (i) contact herbicides; and (ii) herbicides
Table 29.2. Some pre-emergence herbicides used to control weeds in olive

orchards, and their rates of application.
Herbicide
Rate of
application
(g/1000 m2)
Chlorthiamid
600700
Diuron
170
Simazine
300400
Time of
application
Commercial
name
Weeds
controlled
Early winter
or early
spring
Winter/early
spring
Prex
Annual cereals
Casoron
Karmex
Winter
Gesatop
Broadleaf plants
Cereals, annual
broadleaf
plants
Cereals, annual
broadleaf
plants
330
Chapter 29
with hormonal activity (Hilton et al., 1969). Contact herbicides destroy the
sprayed part of the weed. One representative of this category is Paraquat.
Hormonal-like herbicides are sprayed on the leaves and are transported to the
rest of plant. Therefore, these are effective in killing perennial weeds, which
have rhizomes. The rate of herbicide application depends on various factors,
such as age of tree, weed species, irrigation and soil properties.
In olive orchards with weeds a mixture of post-emergence herbicides is
often used, e,g. (i) diuron + simazine + aminotriazole; the rate of application of
aminotriazole is 350500 g/1000 m2; or (ii) dimezon + simazine + Paraquat
(gramoxon); the rate of application is 50100 g/1000 m2.
The use of herbicides is permitted in trees of 4 years old and above, but should
be avoided in gravelly and light soils, since tree damage is possible. For control of
existing weeds (cereals or broadleaf) the systemic herbicide Roundup (glyphosate)
(UCIPM, 2001) is used, at a rate of 90180 g/1000 m2. Furthermore, the
herbicide Paraquat (gramoxon) could be used to control broadleaf weeds and the
annual cereals at a rate of 50100 g/1000 m2.
During spring, when there are perennial weeds such as Cynodon dactylon,
Convolvulus, etc., the dose of Roundup required is 200480 g/1000 m2; and for
Cyperus rotundus the herbicide MSMA (Veliuron) is used at a concentration of
250 g/1000 m2. For the weed Rubus the herbicide Fenoprop (Kuron) is used at
250700 g/1000 m2, in early August. In modern chemical weed control the
following regimes are common:
1. Use of new herbicides with slow release of the active ingredient.
2. Use of hormone-like herbicides.
3. Study of herbicide behaviour regarding their duration within the soil and
their ease of transport in the soil or air.
4. Simazine as a pre-emergence herbicide and glyphosate as a post-emergence
one are the two most important herbicides applied in olive orchards in order to
control Amaranthus blitoides and Lolium rigidum. Simazine is a pre-emergence
herbicide that inhibits electron transport in photosynthesis; glyphosate is a
post-emergence herbicide that inhibits the biosynthesis of aromatic amino
acids. Glyphosate, when applied to leaves, inhibits their elongation and causes
deterioration of the apical part of the fruit. Simazine, when it was sprayed on A.
blitoides, achieved complete control for 20 days at rates of 1.2 and 4 kg/ha.
Glyphosate as a post-emergence herbicide is very efcient for both weeds.
5. Paraquat is recommended for the control of cereal and broadleaf weeds, as
is triuralin.
6. Ansar 529 HC is recommended for the control of Sorghum halepense and
Cyperus rotundus. The rate of application is 650 g/1000 m2 diluted in 3040 l
water.
Olive is cultivated mostly in dry and hilly areas and is subjected to intense
competition from various weeds for water and nutrients. Due to the presence of
weeds, the use of harvesting nets is not easy. Furthermore, the weeds constitute
331
a shelter for pests and olive diseases but, if their height is controlled by cutting,
weeds could be useful, since they add organic matter to the soil and help protect
it from erosion.
In olive orchards weed control is achieved by various strategies: (i) soil
cultivation; (ii) frequent cutting of weeds; (iii) use of post-emergence herbicides;
and (iv) biological means of weed control.
Soil cultivation
This is the most common method of controlling weeds. The use of agricultural
machinery to divide the rhizomes into small pieces only makes the problem
worse, and frequent soil cultivation increases production costs.
Use of grass-cutting machines

This method is efcient for annual weeds but not for woody species. Weeds
should be cut early, before producing ripe seeds. The cut weeds remain on the
soil surface (mulching), to decompose with time.
One of the systems that can be used is non-cultivation of the soil combined
with frequent cutting of weeds, in order to reduce competition and the trees
requirements for water and nutrients. The sensitivity of some common weeds
of olive groves to various herbicides is presented in Table 29.3.
INTEGRATED WEED MANAGEMENT

Integrated weed control is a combination of chemical, biological, cultural and
other means, the application of which reduces the weed population to such a
level that is not antagonistic to olive culture. Furthermore, it maintains
environmental balance in the agro-ecosystem.
Parameters of integrated weed management

Preventive measures
1. Control of weeds before orchard establishment; fumigation of planting
material before establishment in orchard.
2. Cleaning of agricultural machinery before use.
3. Use of decomposed manure for olive orchard fertilization.
4. Control of the purity of cover crop seeds.
332
Table 29.3. Sensitivity of selected weeds to current herbicides.

Weed
Aminotriazole
S, sensitive; I, intermediate; T, tolerant.
S
I
S
I
T
S
T
S
I
T
T
T
T
S
T
T
T
T
IT
IT
T
T
IT
IT
T
T
S
T
T
S
I
I
I
T
I
I
T
T
S
S
T
T
T
T
I
D-NOC
Dinoseb
Diquat
Paraquat
T
T
I
I
S
I
T
T
T
T
T
I
I
S
I
T
T
T
T
T
I
I
S
I
T
I
I
T
I
I
I
T
T
T
Chapter 29
Agropyron repens
Cynodon dactylon
Cirsium arvense
Convolvulus spp.
Polygonum spp.
Rumex spp.
Sorghum halepense
Rubus spp.
Ranunculus spp.
Bromacil Carbetamide Chloroamide 2,4-D
333
Cultivation measures
1. Establishment of rotation crops, as a fundamental agricultural practice.
2. Balanced fertilization.
3. Mulching. Mulching of the in row has certain advantages and disadvantages.
Advantages: (i) increase in organic content of soil; (ii) increase in soil

biological activity; and (iii) reduction in both water evaporation from the
surface and in soil erosion.
Disadvantages: (i) increase in the risk of frost damage; (ii) it should be
carried out every year; (iii) the mulch may be blown away by strong winds;
and (iv) non-composted plant material may spread new weeds or create a
re hazard.
4. Cover crops and permanent sward. Weed control in rows can be achieved
by sowing a permanent sward that shades the soil and absorbs the available
moisture of the soil surface. Thereby, surface sward reduces weed germination.
Mechanical control
There will be varying effects of various soil cultivation systems on the
biological cycle of weeds, to nutrient cycling, to the availability of soil moisture,
on the microclimate and on pests and diseases.
Biological control
1. Production of bio-herbicides from various microorganisms.
2. Production of phytotoxins.
3. Sowing of leguminous plants in order to control perennial weeds.
4. Vapour fumigation of the soil at 70C before planting.
5. Allelopathy.
6. Use of parts remaining from cultivated plants having biological activity
against the weed seeds in olive orchards.
7. Crop rotation by selecting crops having an inhibitory effect on weeds.
Future perspectives of integrated weed management

Integrated weed management is the new strategy in the area of plant
protection and is compatible with future economical, environmental and
social conditions.
30
PESTS AND DISEASES
INTRODUCTION
The olive tree is attacked by various insects, fungi, bacteria and viruses. The
most serious attacks include those by Bactrocera oleae (Dacus oleae), Prays oleae,
Saissetia oleae, Hemiberlesia lataniae, Frankliniella occidentalis, Spilocaea oleaginea,
Verticillium dahliae, Mycocentrospora cladosporioides, Pseudomonas syringae pv.
savastanoi and Armillaria mellea. Control of pests utilizes various methods, e.g.
cultivation (El-Hakim and Kisk, 1988), biological (El-Khawas, 2000) or IPM
(Elmore et al., 2001). We will begin by studying insect pests, followed by fungal,
bacterial and nally, viral problems.
INSECTS
Bactrocera oleae (Gmelin) (olive fruit y, Diptera: Tephritidae)
The olive fruit y, Bactrocera oleae (Gmelin) or Dacus oleae, is a very damaging
pest of olive fruit in most olive-growing countries. The damage is caused by the
Dacus larva, which drills tunnels in the fruit pulp resulting in 30% loss of the
crop. The adult Dacus feeds on nectar, honeydew and other liquid food sources.
Dacus oleae was rst detected in California in 1998.
Morphology
The adult female is about 5 mm long and has a wingspan of approximately
10 mm. The wings are transparent, marked with brown and have a spot at
each tip. The thorax is black, with three parallel lines. The abdomen is black,
covered with grey pubescence. The inner portion of the scutellum is black, the
posterior portion is yellow and odours are emitted by the adults
(Economopoulos et al., 1971). The sheath of the ovipositor is black, with the
ovipositor itself reddish in colour.
335
336
Chapter 30
Life cycle
The insect has two to ve generations per year in Mediterranean countries. It
survives in the pupal form and overwinters several centimetres below the soil
surface, the rst adults appearing from March to May, depending on
temperature and latitude. During summer a period of 610 days elapses before
mating, and this period is longer with lower temperatures. Starting in June, the
female of Dacus lays ten to 12 eggs daily, one per olive fruit, and the total
number of eggs laid during the lifespan of an insect (17 months) is 200250.
The females deposit their eggs beneath the fruit skin with their ovipositor. From
each egg a legless larva derives, which feeds on fruit pulp; this results in fruit
drop (see Fig. 30.1). The durations of egg, larval and pupal stages are 24,
1014 and 10 days, respectively. Mating occurs near the end of the daylight
period. Females, in order to attract males, produce a multi-component
pheromone containing 1,7-dioxaspiro(5.5)undecane, which is an attractant
for male Dacus. Females mate several times during their lifetime. Pheromone is
produced also by males and attracts other males but not females.
(a)
(b)
Fig. 30.1. Olive fruits damaged by Dacus oleae, olive fruit y: (a) galleries drilled
by Dacus pupae, and a pupa; (b) opening created by the exit of the perfect insect
Pests and Diseases
337
Control
Various ways of controlling Dacus have been used (Collier and Van Steenway,
2003), including -ray male sterilization (Economopoulos, 1972), mass
trapping (Haniotakis et al., 1986) and use of chemical repellents of females (Lo
Scalzo et al., 1994). The most common method is the use of insecticide in bait or
air sprays. Other means, more acceptable environmentally, include the use of
radiation to sterilize males, pheromones and biological methods. The required
radiation for sterilization of both sexes is 812 Krad. Furthermore, synthetic
analogues of pheromone have been produced, such as 1,5,7-trioxaspirol(5.5)
undecane, which is equally as attractive as the natural compound but does not
last in the traps as long.
In order to use synthetic pheromones, small pieces of paper or plywood are
dipped in an aqueous (0.1%) solution of deltamethrin for 15 min, then sex
pheromone or ammonium bicarbonate added both Dacus attractants. This
method provides a low-cost means of control. Other methods include the use of
traps for trapping adult insects, biological control and cultivation (early fruit
harvesting).
Acetylcholinesterase (ACE) is the molecular target of organophosphate
(OP) insecticides, and two mutations conferring different levels of OP
insensitivity have been identied in the olive y Bactrocera oleae. Numerous
sensitive and two insensitive alleles have been described from the worldwide
distribution of the y. The insensitive alleles probably reach a high level in
the Mediterranean region, reaching frequencies of up to 100%. The high
frequency of insensitive alleles occurs in areas where OPs have been used
extensively.
There is little knowledge of the chemistry, enzymology or
molecular biology of the biosynthesis of fruit y pheromones to facilitate
species-specic monitoring and control (Mazomenos, 1983). Studies with
Bactrocera oleae suggest the operation of a single, major biosynthetic pathway.
The major component of the pheromone is racemic 1,7-dioxaspiro (5.5)
undecane, which is accompanied by low levels (3%) of hydroxyl derivatives.
Adult olive fruit y populations are monitored using yellow sticky traps
baited with sex pheromone and/or ammonium bicarbonate. Sex pheromone
attracts male Dacus insects, while ammonium bicarbonate attracts mainly
female insects. Females need protein and are attracted by ammonia, which is a
product of protein decomposition. The yellow colour of the trap attracts both
male and female insects. The population of insects trapped is a function of
insect population, temperature and humidity of the atmosphere. Another use
of this trap is to determine the time of insecticide application, by monitoring the
population of Dacus trapped.
For control by insecticide, two methods are available: (i) full coverage
sprays; and (ii) bait sprays, both methods using OPs, such as dimethoate or
fenthion. The number of sprays depends on temperature and humidity, or
PHEROMONES
338
Chapter 30
rainfall during the summer, and involves one to two cover sprays and/or six to
seven bait sprays. The number of sprays is smaller (two to three) in hot and dry
areas. However, the number of sprays depends on the insect population caught
in the traps and whether the fruit is destined for table olives or olive oil
production.
Organophosphates are replaced by the insecticide Spinosad in bait sprays,
since this is a microbial product, very effective against Dacus, but with low
toxicity to humans and animals. Spinosad is mixed with the new fruit y bait
known as GF-120, and applied weekly from mid-June up to just before
harvesting time.
Another insecticide of low toxicity to vertebrates is a pyrethroid compound
(deltamethrin) applied on yellow plywood or paper strips, plus sex pheromone
and/or ammonium bicarbonate. Dacus is attracted and killed by the lethal dose
of pyrethroid.
In recent years the biological control of the olive fruit y has commenced.
In the Mediterranean area Dacus oleae is parasitized by the insect Psyttalia (or
Opius) concolor. This was introduced to Italy, France, Greece and then
California. However, P. concolor is ineffective against Dacus, since there is no
synchronization between the biological stages of the two species, i.e. when
female P. concolor appears in the spring, no fruit y larvae are available.
Saissetia oleae (olive black scale, Hemiptera: Coccidae)

Saissetia oleae or black scale is a serious pest in many areas around the world
(Katsoyannos, 1992; Van Steenwyk et al., 2002; Kovanci and Kumral, 2004;
UCIPM, 2004; Teviotdale et al., 2005). It is native to South Africa and is
widespread throughout Mediterranean countries and California. The same
scale can also be found in other fruiting trees such as g, citrus, apple, etc.
Morphology
The colour of the young adult is brown, becoming black later. The shape of
mature females is hemispherical, and their size is 25 mm long 14 mm
wide. The characteristic feature of this pest is the appearance of an H shape on
its back. The insect lays eggs under the female scale; their length is 0.3 mm and
their colour becomes pink within 23 days initially, and reddish orange before
hatching. From the eggs after hatching nymphs appear, small in length
(0.4 mm) and yellow to brown in colour. These nymphs start to crawl for some
days until they nd a suitable site for feeding. They are called crawlers and,
after a period of feeding lasting 38 weeks, their size doubles to 1.01.3 mm,
with a recognisable dorsal H (second instar nymphs). The next stage is the
immature adult stage, which is 2 mm in length and brown; lastly, the pest
enters the adult stage. The laying capacity of one female black scale is from
1200 to 4000 eggs.
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339
Life cycle
This species overwinters in the form of a nymph and early in spring becomes
adult, starting to lay eggs in May. After hatching crawlers appear in July,
feeding on leaves, shoots or sometimes on fruit. The insect has one or two
generations depending on weather conditions, pruning and pest control.
Economic damage
Since the insect feeds on tree sugars, these trees become weak, leaf abscission
occurs and ower bud differentiation is signicantly reduced. Furthermore,
young scales produce honey-like substances. This leads to a secondary
infection by the fungus Capnia, resulting in deterioration of fruit quality and a
decrease in photosynthesis.
Control
The insect can be controlled by cultivation practices, insecticidal sprays or by
natural enemies of the black scale.
PRUNING In trees left unpruned or lightly pruned and a relatively dense
canopy, damage and infestation are severe. Regular pruning exposes the black
scale to hot and dry conditions and increases its percentage mortality.
However, if the weather is cool and humid this favours black scale, especially
when the tree canopy is dense. In unpruned olive trees the shady and moist
environment inside the tree canopy protects the scale, which can then survive
even very hot summers. Furthermore, irrigation may create favourable
conditions for the black scale population to increase.
Due to the existence of the scales covering, pesticides are

effective only at the crawler stage during the summer. When the population of
black scale is low to medium oil treatments during the dormant period are
effective. The most effective pesticides are OPs.
INSECTICIDES
There are many natural enemies, such as Metaphycus

helvolus, M. bartletic, Scutellista cyanea, Chrysoperla spp., Hippodamia convergens
and Hyperaspis sp. From these enemies only Hyperaspis spp. can signicantly
reduce the population of black scale.
NATURAL ENEMIES
Prays oleae (Bernard) (olive moth, Lepidoptera:

Yponomeutidae)
This insect is known also as olive kernel borer. It is widespread in the
Mediterranean area and in other olive-growing countries, causing severe
damage, though a little less harmful than the effects caused by Dacus. The
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moth feeds on olive trees, cultivated and wild, and all olive cultivars are prone
to damage if no control measures are taken. Other species where this moth can
be found include other genera of the Oleaceae family such as Ligustrum,
Jasminum and Phillyrea. Prays oleae has three generations: the rst on oral
buds, the second in the fruit endocarp and the third on the leaves where the
insect overwinters.
Life cycle
The insect overwinters as an adult and appears in spring; the females deposit
their eggs in the calyx of owers. The larva, appearing after 912 days, feeds on
the oral parts initially on anthers and subsequently on stigma, style and
ovary. The larvae later connect the entire inorescence with a silky thread. The
second generation attacks the young fruits close to the stalk and the larva
enters the endocarp, where it stays for 80135 days. After this period the larva
leaves the endocarp and exits via the same opening, resulting in fruit drop. The
adult insects appear after 814 days. The third generation lays eggs on the
upper leaf surface. The hatched larvae (714 days) enter the leaf by drilling
tunnels and digest foliar material. At the end of winter the adults appear and a
new cycle begins.
Economic damage
The damage caused by this moth ranks second only to that of Dacus oleae, and
is serious with high insect populations or low owering conditions. Damage
affects owers, fruits and leaves and results in fruit drop.
Chemical and biological control
Unfavourable climatic conditions greatly limit the
population of this insect. Pruning and maintenance of open canopies reduce
RH, and with RH below 50% or with temperatures above 30C egg death
results. That is why this insect does not appear in dry areas.
CULTIVATION PRACTICES
CONTROL The insecticidal bacterium Bacillus thuringiensis

Berliner (subp. Kurstaki) when sprayed is very effective in reducing larval
populations. Furthermore, the insect Chrysoperla carnea Stephens is a natural
enemy of P. oleae, since it is predatory to the insects eggs, larvae and pupae.
BIOLOGICAL
CHEMICAL CONTROL
The use of insecticides such as dimethoate controls
population levels.
The use of female sex pheromones is useful as a control method
(Campion et al., 1979; Kavallieratos et al., 2005).
PHEROMONES
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341
Palpita unionalis (Hbner) (jasmine moth, Lepidoptera:

Pyralidae)
Life cycle
Palpita unionalis is active at night; mating lasts 13 h and eggs are laid at
twilight. The eggs are laid singly or in series on the lower leaf surface. Newly
hatched larvae feed on young, tender leaves, and older ones on the
parenchyma of established leaves. The larvae spin several leaves or parts of
leaves together with silken threads. The mean total life of the rst generation is
4548 days, while that of the second generation is 38 days (AlexopoulouVassilaina and Santorini, 1973; El-Ki et al., 1974; Amin and Saleh, 1975;
Badaawi et al., 1976; Shehata et al., 2003). The larvae drill tunnels having a
crescent-like shape, and can also infect inorescences and young fruits causing
fruit drop, since they feed on the seed embryo.
Control
BIOLOGICAL CONTROL Bacillus thuringiensis has toxic effects on moth larvae
when they feed on it. Use of the commercial product marketed under the name
Dipel 2X at a concentration 0.4 g/l achieves 100% eradication of the larvae
within 48 h.
CULTIVATION PRACTICES Sucker eradication reduces the problem, since the
insect prefers to lay its eggs on the suckers. The larval stage lasts 16 days at
1723C and 15 days at 21.625.5C for the rst and second generations,
respectively. The number of eggs laid per female ranges from 630 to 658 and
from 425 to 496 for the rst and second generations, respectively.
CONTROL Oil spraying is effective against light to moderate
infestations, when used in conjunction with pruning to open the orchard canopy.
Oil is not sprayed between 20 August and harvest for olives intended for green
ripe processing, since that causes fruit spotting. It is preferable to spray at night or
early in the morning if the temperature is greater than 32C during the day.
When the infestation is severe, the insecticide Carbaryl (Sevin 80S) is
included in the spray solution. This spray is, however, very destructive to most
natural enemies of Palpita. Another insecticide that might be incorporated is
Methidathion (Supracide 25WP).
CHEMICAL
Frankliniella occidentalis (Pergande) (western ower thrips,

Thysanoptera: Thripidae)
This insect is also known as western ower thrips. Many plant species are hosts
of this insect.
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Morphology and life cycle

The adults of F. occidentalis are very small insects (1 mm). Their colour ranges
from white to yellow, with small brown spots on the abdomen. The thorax is
orange in colour and the abdomen is brown. Adults overwinter in weeds and in
other places in the orchard. During spring they lay eggs and deposit them in
buds, shoots and owers. After hatching, nymphs feed on shoots, fruits or
leaves. The nymphs, after attaining full size and development, are ready to
pupate; they drop to the ground and pupate in protected places. The maximum
adult population occurs in June and, as the weeds dry out, the adults move to
various crops such as olive trees. This insect has ve to six generations per year.
The damage is due to the fact that thrips feed on leaves and shoots but, because
they absorb sap from the fruits, the affected fruit shows scars and is rendered
unsuitable for processing. This problem is serious with green olives intended
for processing.
Control
Various insecticides are recommended for thrips, and cultivation techniques
are very effective. One such technique is to disc the area close to the olive grove
that is covered with weeds, to avoid an increase in thrips population and
subsequent migration to olive orchards. Managing the vegetation in and
around olive orchards is important in reducing the potential for damage from
western ower thrips. Avoid discing orchard cover crops while trees are in
bloom. Spray treatments are applied at full bloom.
Parlatoria oleae (Colve) (olive scale, Homoptera: Diaspididae)

Parlatoria oleae is also known as olive scale. This is widespread in the
Mediterranean countries, China, the Middle East, India, Turkey and the USA
(California, Arizona). This is a serious pest and requires systematic pesticide
treatment every year to reduce economic losses. Parlatoria can be found in
about 200 plant species.
The adult is 2 mm in length with a greyish oval covering. The young females
body under the scale cover is reddish to deep purple; the covering of the male is
elongate, white and at. The insect has two generations per year and
overwinters as an immature mated female; the overwintering forms begin to
lay eggs in early May. The emergence of the rst-generation crawlers occurs in
late June to early July, when the young fruits are attacked and deformed. The
second generation in August causes semi-circular dark purpleblack scales on
the green fruits; this damage is severe in table olives. Infestation may also occur
on branches and leaves, which results in a signicant decrease in productivity.
Pests and Diseases
343
Economic damage and control

This insect can cause leaf abscission, drying of twigs and reduction in
photosynthesis; however, the most serious damage is that on the fruits (Foda,
1973). These become disgured, with purple spotting on green olives, which
then become unsuitable for processing (see Fig. 30.2). Parlatoria can be
attacked by two parasites, i.e. Aphytis maculicornis MASI and Coccophagoides
utilis Doutt. If chemical treatments are necessary two sprays are required, the
rst in late MayJune for the rst generation and JulyAugust for the second
generation. Application should be carried out when crawlers appear.
Hemiberlesia lataniae (Signoret) (latania scale, Hemiptera:

Diaspididae)
Morphology
Latania scale is a scale similar in size to the adult olive scale. The difference is in
the waxy shell covering, which is more conical, with a small black spot to one
side of the centre. Also, the body of the female is yellow while it is reddish purple
on the olive scale. The insect produces many generations per year.
Life cycle
The insect overwinters in the form of nymph (second instar). Early in spring it
matures and the female insect lays eggs in batches of 1520, located beneath
the scale covering. After hatching the crawlers start to feed, appearing in May,
Fig. 30.2. Olive fruit damage in the form of spot due to Parlatoria oleae, olive scale
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July and September. Latania, except on olive trees, feeds on avocado, acacia,
euonymus, kiwi fruit, rose and other species.
Economic damage
The scales infest leaves, bark and fruits, producing a dark purple spot of very clear
outline. Infested fruits, especially for table olive processing, become worthless.
Chemical and biological control
For biological control the species Aphytis melinus and other parasites are used.
However, where chemical treatment is necessary oil sprays or oil sprays plus
Sevin 80S or Supracide 25WP are used. Spraying is performed in late May and
in late July to August. The best results are obtained when scale crawlers start
moving onto the fruit. Treatment between these two sprays is not necessary.
Furthermore, a postharvest treatment (October/November) is also effective.
Spraying is effective, especially when used in conjunction with pruning to open
the orchard canopy.
Aspidiotus nerii (oleander scale (Bouche) or ivy scale,

Hemiptera: Diaspididae)
Many plants are hosts to this insect such as camellia, cherry, grapefruit, lemon,
magnolia, orange, rose and olive, where infestations may damage the fruit.
It resembles greedy scale, the only difference being that the covering of its scale
is less convex. It overwinters in the form of adult females, which start to lay
eggs in April. The insect has two generations, in April and in July/August.
During heavy infestations this scale infests olive fruit, producing green spots on
purple fruit and certain deformations. The infested fruit becomes worthless.
Biological control agents may decrease the scale population.
Phloeotribus scarabaeoides (Bernard) (olive beetle,

Coleoptera: Scolytidae)
This is known also as olive beetle and is present in all Mediterranean areas. The
adult is 2.02.5 mm long.
Life cycle
This insect overwinters in the form of either larva, nymph or adult in shoot
tunnels. The female drills galleries in the branches and trunk. In these
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345
channels mating takes place and the female lays eggs. Within 12 weeks larvae
appear, which open small channels under the bark. In another 34 weeks the
larva is transformed into an adult, which leaves the branch. The insect has four
generations, the adults appearing in March/April, AprilJuly,
August/September and AugustNovember. The infested trees have low vigour
and low productivity.
Control
Eradication of the pruning material by burying, before the insect lays its
eggs.
Burning the suckers, which can host a large number of larvae.
Keeping the trees healthy by appropriate fertilization, irrigation and
pruning.
Application of organophosphate insecticides is recommended when the
infection is severe.
Oxyenus maxwelli (K.) (olive mite)

This mite is widespread in Mediterranean and other countries.
It is very small in size, with four legs, with body colour yellowish to orange. It
overwinters in the form of adult in the tree bark. The females lay eggs in early
spring through to summer. During periods of high temperature and low
humidity its population is reduced.
It infests mainly young olive leaves, these leaves developing a silver colour and
longitudinal curl. The mite also infests inorescences, causing pistil abortion.
Some of the symptoms due to infestation include inorescence abscission and
necrosis and drop of buds. In cases of severe infestation chemical control is
recommended.
Zeuzera pyrina L. (leopard moth, Lepidoptera: Cossidae)

Damage due to this moth has been reported in species from over 30 botanical
families. In olives the insect is present mostly in Mediterranean countries, and
also Syria. The insect can damage all olive varieties: resistant varieties have not
yet been reported.
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Life cycle
Adults lay their eggs during summer and, after hatching, the larvae attack
lateral buds and drill sub-cortical channels. The larvae exit the branch by
creating a hole and subsequently enter a new branch. Their presence is
obvious by the sawdust released from the opening (frass). The biological cycle
lasts 1 year, with larvae being fully developed in late winter and overwintering
in the form of chrysalids. The adults occur in May to June.

The tunnels opened cause serious damage and young trees may dry out. The
secondary branches also die and tree vigour is reduced. For control we immerse
a piece of cotton wool in a liquid producing toxic vapours and with that we plug
the opening of the tunnel. Furthermore, in young trees spraying with systemic
insecticides may solve the problem.
FUNGAL DISEASES
Spilocaea (or Cycloconium) oleaginea (olive peacock spot)
This disease is known as olive peacock spot, peacock spot and/or birds eye or
cycloconium, and is caused by the fungus Spilocaea (or Cyclonium) oleaginea
(Cast) Hughes. The disease is common in many areas of the world.
Symptoms
The fungus attacks leaves, fruit and fruit-bearing stems, the symptoms being
observed most often on the upper leaf surfaces of leaves located in the lower
part of the tree canopy (Graniti, 1962; Proietti et al., 2002a). Peacock spot
causes cutin degradation, appearing on leaves in the form of sooty blotches
having a diameter of 26 mm (Sparavano and Graniti, 1978). The colour of
these spots changes to muddy green and later to black, with a yellow halo,
giving the appearance of the eye spot on the peacocks tail feathers (see Fig.
30.3). One leaf may have more than one spot, which causes the infected leaves
to abscise. Leaf abscission reduces productivity and enhances biennial bearing.
New spots appear in late winter to early spring. Outbreaks are sporadic and the
disease may need many years before it causes economic damage. In the
margins of the spots spores develop, high temperatures hindering spore
germination. Therefore, high temperatures inactivate the disease. Cultivar and
pruning intensity also affect the attacks of Spilocaea oleaginea (Tombesi and
Ruffolo, 2006).
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347
Fig. 30.3. Olive leaf infection by Spilocaea oleaginea (peacock spot). The infection
appears to be in the form of spots, resembling the eye of a peacocks tail feather
Life cycle and control

The fungus survives on the infected leaves that do not abscise. During autumn
the margins of the spots grow and produce conidia. Rainfall or watering
transports the conidia to the lower part of the canopy, where they infect the
lower leaves. The optimum temperature for conidial germination is 21C, while
temperatures greater than 30C hinder germination. Infection during winter
requires more time for development of symptoms in comparison with spring
infection. A Cu-based fungicide applied in late autumn, before the rainy
season, provides adequate control; sometimes, a second spray is applied in
January, but its effectiveness is questionable. Furthermore, later spraying does
not protect against Spilocaea oleaginea. It is important to apply the Cu fungicide
yearly.
Complete coverage is necessary, requiring a power sprayer with high
pressure to cover the leaves on both sides, especially in the inner parts of the
tree. Both old and young leaves are sensitive and, furthermore, most varieties
are susceptible. If rainfall is frequent, both winter and spring infections may
occur. Peacock spot is not affected signicantly by the level of tree nutrition.
However, high N and low Ca concentrations render the tree more susceptible to
infection. The forms of Cu that can be used to protect olive trees from peacock
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spot include copper sulphate tribasic, copper oxide, copper hydroxide and
copper oxychloride. Infection is worst in areas of low elevation, under
conditions of heavy dew, fog or high humidity, under a dense tree canopy and
following excessive sprinkler irrigation.
The guidelines for effective spraying programmes include the following:
Spraying is effective if carried out twice a year, in late autumn and in early
spring.
Spraying should be carried out before any rain and, should it rain within
2 h following application, a further spraying is necessary.
Avoid spraying during the owering period, since Cu may negatively affect
budding and fruit set.
When the level of infection is high (> 10% of leaves infected) the recommended combinations are: (i) a combination of Stroby WG (1015 g/
100 l) + Cuprox (500 g/100 l); or (ii) Stroby WG (1015 g/100 l) +
Kocide 2000 (190 g/100 l), both combinations being mixed prior to
spraying. The volume of spray solution per 1000 m2 orchard is 100150 l.
Verticillium dahliae (olive verticillium wilt, Hypocreales:

Incertae sedis)
The pathogen of this disease is the fungus Verticillium dahliae Kleb. This is a
serious disease causing signicant damages in olive culture and killing both
young and mature trees. It is widespread in many areas of the world
(Thanassoulopoulos et al., 1979; Bellahcene et al., 2000, 2005).
Symptoms
The leaves on some branches suddenly collapse and die early in the growing
season, the symptoms becoming more acute as the season progresses (Cirulli,
1981; Blanco-Lpez et al., 1984). The vascular tissues exhibit little or no
discoloration and the dead leaves do not abscise.
Life cycle
The fungus V. dahliae is very common in many areas and in many agricultural
soils. It survives in both soil and infected roots from year to year (Schnathorst,
1981; Tjamos, 1993). The form surviving in the soil is the microsclerotia,
which are formed inside the infected plants and, as tissues decay,
microsclerotia are liberated into the soil. Microsclerotia remain inactive for
some time, and their germination starts when the roots commence growth.
The fungus enters and grows into the vessels of the current years growth,
resulting in wilt and death of the plants. Verticillium infection occurs mostly
during late winter and spring, when the soil is cool and having adequate
moisture. Rising temperatures decrease levels of Verticillium infection. Another
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349
wave of infection occurs in early autumn, when the soil cools again. High
summer temperatures kill the fungus. The fungus has various hosts, such as
cotton, tomato, pepper, melon, stone fruits and pistachio.
Management and control
The most important way of protecting trees from Verticillium wilt is the
avoidance of the fungus. Therefore, soils that have previously been planted for
a number of years with crops very susceptible to Verticillium, i.e. aubergine,
etc., should not be given over to olive. Furthermore, Verticillium spores and
inocula can be reduced in number prior to planting by soil fumigation or
disinfection by solar energy. Other methods of control include cultivation of
grass cover crops or by use of a combination of the above methods. When we
plant olives in an area where previously susceptible perennial plants have been
grown, it is necessary to remove most of the existing root system. With regard
to rootstocks not resistant to Verticillium wilt most were reported to be so, apart
from Olea oblonga and the cultivar Ascolano.
For solar disinfection the soil is covered with a transparent plastic lm
throughout the summer, when increased temperatures kill the fungus. For soil
fumigation we withhold water during the summer; the drier the soil the more
efcient is the penetration of methyl bromide. Methyl bromide is injected to
4575 cm depth and is covered with a gas-proof cover; the greater the dose of
methyl bromide the greater the depth of penetration. The cover remains in
place for two weeks, and afterwards it is removed and the soil is aerated for
1 month before planting. Methyl bromide may be applied from late summer to
early autumn. It is generally accepted that soil fumigation is effective in
nursery soils but is not appropriate in existing olive groves.
Armillaria mellea (armillaria root rot, oak root fungus,

Agaricales: Marasmiaceae)
Armillaria root rot is not a serious disease of olive trees, although the fungus
can survive for decades in soils on dead roots. The pathogen involved is
Armillaria mellea (Vahl.) Quel.
Symptoms
Infected trees become weak and exhibit low vigour. This symptom appears
initially on one side of the tree only, but over several years it gradually involves
the whole tree. Discoloration of the bark and outer wood of the roots and tree
crown is obvious. Therefore, the colour of infected roots with A. mellea is white
to yellowish, fan-shaped mycelia appearing between the bark and wood.
Another symptom is the appearance of brown to black rhizomorphs and
mushrooms at the crown of Armillaria-infected trees.
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Armillaria mellea is very common in soils where oak trees previously grew. The
fungus lives for years in the dead roots of trees, providing the soil is not dry. As
a root grows it comes in contact with infected roots and the disease is spread
thus through the orchard, from tree to tree. The infected roots represent
inocula retained in the soil prole. To reduce the danger from A. mellea root rot
we avoid planting olive groves where forest or oak trees were recently grown.
Another technique in infected soils is to reduce irrigation so that the crown and
the upper root system grow in a dry soil. If there are any infected trees these
should be excavated and the area should be fumigated in order to delay the
spread of disease. With regard to rootstocks, no resistant examples exist, and
damaged trees cannot be treated.
Many cultivation practices are applicable in controlling this fungus.
Initially, the soil from around the base of the tree is removed to a depth of
2030 cm, creating a small depression. In this way the trunk is exposed and
the upper roots and crown become dry. Furthermore, during winter the
collection of rainwater in the depression is avoided by appropriate drainage; the
depression remains open for a number of years.
Another technique is fumigation, which is very efcient in shallow soils; in
deep or heavy clay soils soil fumigation is not efcient. It is necessary before
fumigation to remove all infected trees and a signicant part of their root
system. Sometimes it is necessary to remove trees adjacent to those having
symptoms, since it is likely that these trees will also be infected. The infected
material should be burned. Methyl bromide is injected to a depth of 4575 cm
and the soil is covered with a gas-proof material that remains for 2 weeks, after
which it is aerated for 1 month before planting.
An alternative method is to dry the soil by reducing irrigation during the
summer or by using cover crops such as safower or sudan grass.
Mycocentrospora (Cercospora) cladosporioides (Hyphales:

Deuteromyocotina)
Symptoms
Leaves become slightly chlorotic. The lower side of the leaves is discolored, with
the conidia of the fungus appearing as a black dust, but no spots are evident;
such leaves may abscise. Furthermore, spots appear on the fruits and such
olives are characterized by lack of uniform ripening.
The disease is common in humid areas. This fungus has a similar life cycle to
that of Spilocaea oleaginea. Leaf spotting is more difcult to control than in peacock
spot and requires more careful treatment. A workable solution to the problem is
preventive spraying of olive trees following harvest (before winter rains) and a
second application in spring, if the weather is wet or rainy. Defoliation will result
in less shoot growth, decreased fruit set and poor ower bud formation the
Pests and Diseases
351
following year. High and frequent rainfall increases Cercospora infection. When
the winter is dry problems are minimized.
BACTERIAL DISEASES
Pseudomonas syringae pv. savastanoi (Smith Stevens) (olive
knot, Psedomonadales: Pseudomonadaea)
This pathogen creates rough galls or swellings about 1.25.0 cm in diameter
on twigs, branches, trunks, roots and leaves; galls may also be formed on
trunks or limbs following wounding. Olive knot reduces productivity, since it
destroys twigs and branches (Iacobellis, 2001).
Symptoms
This disease was rst described in olive by the Greek philosopher Theophrastus
(4th century BC). Due to heavy infestation the affected limbs are dwarfed,
defoliated or killed and the whole plant may be stunted. The pathogen is also
responsible for similar diseases of other genera of the Oleaceae family such as
Forsythia, Jasminum, Phillyrea and Oleander (Nerium oleander). Due to infection,
a decrease in production and fruit size is recorded. Furthermore, the green
olives from infected limbs obtain off-avours or rancidity in taste. Other strains
of P. savastanoi include P. savastanoi pv. nerii and P. savastanoi pv. fraxini.
The main symptom is the appearance of rough galls appearing on twigs
and small branches in areas where wounds, leaf scars, pruning cuts and
mechanical injuries exist. These knots, especially at harvest, are very large and
hinder water or assimilate transport, leading to defoliation (Sisto et al., 2004).
The root system is not affected by the fungus; furthermore, stomata and
lenticels are not routes of entry for olive knot bacteria.
The optimum temperature for Pseudomonas infection is 2124C, the
maximum 32C and the minimum 510C. Rainfall encourages infection, the
most susceptible period for infection being OctoberJune. The knots develop
when the tree is actively growing during spring or early summer.
In the knots the bacteria survive and are spread easily by water over the whole
year. Various factors affect P. savastanoi inoculation (Penyalver et al., 2006): the
most appropriate temperatures for infection are the lows in autumn or spring.
The occurrence of infection requires openings provided by leaf scars, pruning
wounds or cracking due to freezing. No tolerant cultivars are known.
For Pseudomomas control it is necessary to make preventive fungicide sprays
to reduce bacterial entry through the scars. Furthermore, pruning should be
conducted carefully during the dry season, in order to remove galls from twigs.
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After pruning disinfection follows, with a Cu-based solution; the required

number of sprays for control is three. The spraying schedule is one in the
autumn, one before the winter rains begin and another in spring, following leaf
abscission. No specic bactericides are available, so infected trees are treated
with Cu compounds or by application of preventive techniques that reduce the
pathogen population (Lavermicocca et al., 2003). Copper sulphate applications
are required in cases such as wounding due to frost, hail, pruning, harvesting or
disease, e.g. peacock spot, Verticillium wilt or Cercospora infection).
Agronomic practices devised for limitation or eradication of the disease
include the following:
Avoid harvesting during rainy days and avoid inicting wounds on the trees.
Use of pathogen-free stock (internally and externally).
Use of stock with a phytosanitary certication protocol.
Use of rapid detection methods for the pathogen.
Use of resistant cultivars (Hassani et al., 2003; Iannotta et al., 2006).
Evaluation of the available olive germplasm to nd cultivars resistant or
tolerant to olive knot disease; this is the most important means of disease
management.
Selection of cultivars tolerant/resistant to freezing.
VIRAL DISEASES
A number of viruses infect olive trees in various areas (Faggioli et al., 2005).
Such viruses include cherry leaf roll (Savino and Gallitelli, 1981a), olive latent
ring spot (Savino et al., 1983), cucumber mosaic virus (Savino and Gallitelli,
1981b), an elongated virus (Faggioli and Barba, 1995) and two necroviruses
(Felix and Clara, 2002). Modern techniques are used for virus detection
(Grieco et al., 2000; Bertolini et al., 2001), and various sanitation methods for
virus infection have been proposed (Bottalico et al., 2004).
31
BIOTECHNOLOGICAL ASPECTS OF
OLIVE CULTURE
INTRODUCTION
Olive culture was spread to all the various countries of the Mediterranean
region accompanying human migration, but in modern times certain changes
must be considered in order to face several issues: (i) cultivar identication; (ii)
regeneration; (iii) maturation; (iv) abiotic stress tolerance; (v) plant size and
architecture; (vi) resistance to pests and diseases; (vii) water and salt tolerance;
(viii) parthenocarpy; and (ix) self-compatibility. Rugini et al. (2006) have
provided an overview of olive biotechnology.
IDENTIFICATION AND IMPROVEMENT OF CULTIVARS

The current trend is to use a small number of cultivars with excellent
characteristics through selection and biotechnology (Guerin et al., 2002) for
the production of table olives and olive oil. The current trend is the production
of mono-cultivar olive oil with excellent characteristics. In Spain, olives have
been managed as self-compatible trees and no other cultivars are included as
pollinators (Cuevas et al., 2001). This means that molecular markers should be
used to distinguish cultivars precisely and not base selection purely on their
morphological characteristics (Hatzopoulos et al., 2002).
MICROPROPAGATION
Some very important olive cultivars such as Kalamon, Frantoio and
Picholine are difcult to propagate in vitro (Zuccherelli and Zuccherelli,
2002). Attempts have been conducted to root such cultivars, and many factors
affecting rooting were performed, such as C:N ratio, B concentration and use of
putrescine, which promotes the activity of total peroxidases. Basal treatments
of explants with H2O2 promote early and improved rooting. Furthermore,
353
354
Chapter 31
improved production of difcult-to-root cultivars can be achieved by basal

etiolation of shoots during rooting and the use of polyamines and H2O2 (Rugini
et al., 1997). The issue of low rooting capacity of valuable olive cultivars could
be circumvented by the use of transgenic plants with the rolB gene of
Agrobacterium rhizogenes, which renders plants more sensitive to auxin.
ABIOTIC STRESS TOLERANCE

Cold tolerance and ozone stress
Climatic changes create problems in olive culture due to frost damage. Most
olive cultivars can resist temperatures no lower than 12C after acclimation
due to freezing-tolerance mechanisms (Thomashow, 1999). However, in
certain olive-growing areas lower temperatures are common. Cold-hardiness
is a very important trait for olive improvement (Bartolozzi and Fontanazza,
1999). Improved cold tolerance could involve one or more of the following:
The superoxide dismutase gene (SOD), which is responsible for repair of

ozone-damaged cells (Van Camp et al., 1993).
The CBF1 gene, which increases tolerance to cold (Jaglo-Ottosen et al., 1998).
The involvement of the cryoprotective proteins COR/LEA/dehydrin (Late
Embryogenesis Abundant proteins). Among the genes that are the most
highly induced during cold acclimation are: (i) the classical COR (coldregulated) genes, alternatively designated KIN (cold-induced); (ii) RD
(responsive to dehydration); (iii) LTI (low temperature-induced); and (iv)
ERD (early responsive to dehydration) (Tomashow, 1998). The proteins
encoded by these genes are extremely hydrophilic and are either novel or
members of the dehydrins (Close, 1997).
Water stress
The protein osmotin is involved in water stress. Xiong et al. (2002) reported on
cell signalling following cold, drought and salt stress. The problems of drought
and salinization could be solved by using drought- and salt-resistant cultivars,
which are also appropriate for high-density plantings.
Salt tolerance
The genes SOS1, SOS2 and SOS3 are postulated to encode regulatory
components for salt tolerance. Do such genes exist in olives? Evidence indicates
a critical role for K in salt tolerance (Zhu et al., 1998). Furthermore, high
salinity affects cellular and molecular responses (Hasegawa et al., 2000).
Biotechnological Aspects of Olive Culture
355
SIZE OF TREE
The new trend of dense plantings in olive orchards requires trees of both reduced
size and altered shape. A number of genes exist in olive for controlling size, and
a number of cultivars should be studied for their possible use as olive rootstocks,
in order to produce trees adapted to mechanical harvesting and/or pruning.
RIPENING OF FRUIT
Many olive cultivars are late in ripening, or their ripening is not synchronous,
creating problems at harvesting time. The introduction of genes that block
ethylene synthesis is very important with regard to timing of ripening.
RESISTANCE TO PESTS AND DISEASES

The need for more healthy products for human consumption, containing no
traces of phytoprotective chemicals in either table olives or olive oil, triggers a
quest for genes tolerant to diseases and pests. Two very serious problems are
Verticillium (verticillium wilt) and Spilocaea oleaginea (peacock spot). Several
ways of improving the olives defence system are outlined below.
Through antifungal genes, genes for hydrolytic enzymes (glucanase,

chitinase) (Broglie et al., 1991) and genes coding for the inhibition of
polygalacturonase.
Resistance to bacteria has been obtained by the introduction of genes
coding for bacteriocidal polypeptides such as thionin. Of prime importance
is the need to nd genes conferring resistance to Bactrocera oleae.
Resistance to Prays oleae, through the creation of plants resistant to P. oleae
by genetic transformation of certain cultivars with toxin protein genes
from Bacillus thuringiensis.
Cycloconium-resistant plants have thicker leaves and could be selected from
tetraploid meristems.
Verticillium-resistant plants obtained by somaclonal variation from a
zygotic embryo callus.
Resistance to viruses and phytoplasmas. Many viruses and phytoplasmas
have been isolated in olive; therefore, the use of healthy plants for new
planting is recommended, and thermotherapy of meristem tip culture is a
useful strategy.
356
Chapter 31
PARTHENOCARPY
Some self-sterile cultivars and others produce a signicant proportion of
parthenocarpic fruits. Therefore, their fruit set and productivity is reduced
since parthenocarpic fruits are very small in size. Introduction of genes that
induce parthenocarpy allow the normal development of parthenocarpic fruit.
QUALITY OF OLIVE OIL

Olive oil quality is determined by its oleic acid and polyphenol contents, both
substances playing an important role in human health. Therefore, by
suppression of oleate desaturase it would be possible to increase the percentage
of oleic acid (C18:1) by almost threefold. The same measures could be
employed to increase the polyphenol content of olive oil.
LOW CHILLING REQUIREMENTS

It is important to nd new varieties with low chilling requirements appropriate
for southern areas. This will help expand the area of olive cultivation.
SELF-COMPATIBILITY
Self-compatible (self-fruitful) cultivars are important in high-density plantings
and for mono-cultivar olive oil production.
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INDEX
Abscisic acid 16, 87, 88, 106, 130, 156,

171173, 214
Abscisic acid accumulation 173
Abscission
chemicals 245
zones of fruit 252
ACC (1-aminocyclopropane-1 carboxylic acid)
172
ACC synthase 254
Acclimatization 144, 146
Acidity 284
Adramitini 219, 262, 267, 268, 290
Adulteration detection 286
enzymatic methods 287, 288
AFLP markers 256
Agar 146
Agiou Orous 46, 260
Aglandau 267
Agrobacterium rhizogenes 146, 354
Alcohols and aldehydes 27, 28
Alkanes 27
Alsol 252
Alternate bearing 39, 105107, 111, 211
Aminization 184
Aminoacids of olive fruit 237
Aminotriazole 330
Ammonication 184
Ammonium absorption 193, 194
Ammonium assimilation, enzymes 196
Ammonium incorporation into aminoacids
186
Amplied Fragment Length Polymorphism
(AFLP) 256, 288
Amygdalolia 268
Anatomy of olive tree 15
buds 19
owers 19, 20
fruit 20
inorescences 19, 20
leaves 17, 18
main branches 17
root system 1517
trunk 17
Anchorage 15, 16
Anthocyanins 25, 26, 29, 238, 240, 241
Antioxidant activity 30, 31, 317
Antioxidants 145, 238, 295, 298, 299, 303,
310, 312, 313
Apical dominance 22, 214, 222, 223
Appropriate soil 52
Arbequina 47, 122, 126, 150, 155, 205,
219, 249, 265, 266
Arbequina IRTA-118 122
Arbosana 122, 147, 249
Armillaria mellea 114, 259, 335, 349, 350
Aromatic compounds 238, 239
Arpa 54
Ascolana tenera 269271
Ascolano 17, 44, 51, 269271, 349
Ascorbic acid 145
Aspidiotus nerii 344
Assessment of frost tolerance 58
chlorophyll uorescence 59
Ec measurement 5861
electrolyte leakage 59
stomata size and density 59
visual observations 58, 59, 62
Authenticity 286, 288
Authenticity standards 283
Bacillus thuringiensis 320322, 355

Bacterial diseases 351, 352
399
400
Index
Bactrocera oleae 259, 265, 267, 269, 270,

322, 335338, 355
Bare soil and frost 62
Bark grafting 141
Bark splitting 55, 56, 139
Barnea (K-18) 106, 265
Basammon 190
Biennial bearing 105, 182, 183, 245
Biodiversity 77, 295, 323, 324
Biological olive culture 319, 320, 323
Biological produce 50
Biotechnological aspects 353
Bitter index 165
Bitterness index 241
Black olives in brine 271
Black ripe olives 272, 274, 276
Blood pressure lowering 304
Blue baby 187
Boron 166, 185, 189, 200, 321, 353
Boron accumulation in olive rootstock 47, 48
Boron adsorption 200
Boron concentration seasonal variation 202
Boron toxicity 201
Botanical species of Olea 11
Bridge grafti ng 142
Brine-cured olives 275
Bronze age 5
Browning
fruits 28, 240
wood and cambium 5456
Bruised olives 274
b-sitosterol 238
Budding 43, 46, 138
Ca/Mg ratio 199

Ca/N ratio 146, 353
Caffeic acid 29, 30, 107, 236, 242
Caffeoylquinic acid 106, 107
Calcium
freezing 57
salt tolerance 214
Calcium channels 214
Calcium roles 57, 199, 214
Californian-type black ripe olives 276
Callus 128, 129, 139
Cambial zone 139
Campesterol 28, 238
Canvases 247
Capillary pore space 64
Carbon dioxide 70, 74
Cardiovascular system 6
Carotenoids 25, 165
olive oil 237, 238, 240, 241, 285, 317
Catechole oxidase 240

CEC 64
Cell division 109
Cell wall softening 240
Chemical composition variation 183
Chemical scarication 127
Chemical signaling 172
Chemical weed management 325
Chill-heating model 94
Chilling 19, 81
Chilling requirements 53, 54, 81
Chip budding 138
Chip grafting 138
Chlorogenic acid 106
Chloroplast pigments changes 240
Chondrolia Chalkidikis 46, 81, 87, 127, 129,
157, 190, 202, 205, 217, 219, 267,
268, 272, 276, 290
Chromatography 287
Cinnamic acid 107
Classication criteria of olive cultivars 255
Clay minerals 63
Clay soil 52, 64
Cleft grafting 140, 141
Climate 51
Climate change 73, 75, 93
Climate change adaptability 80
Climate change side effects 76
Climatic impact 79
irrigation 79
phenology 79
Cold acclimation 57, 58
Cold frame 128
Cold Regulated Protein (COR) 354
Compatible solutes 175
Compost from moist pomace 297
Conductivity 61, 152
Consumer countries of table olives 37, 38
Continuous cycle 279
Conventional cultivation 319, 323
Conventional methods 286288
Coratina 58, 219, 266
Countries of cultivation 10, 11
Cover crops 321, 322
Cretan civilization 2
Crop coefcient 154
Cross-pollination 53, 99
Crude pomace oil 281
Cryopreservation 143, 149, 150
Cryoprotective protein (CPR) 354
Cryoscopy 69
Cryprotective substances 57
Cultivar selection 113
Index
Cultivars taxonomy 256
Culture areas 78
Culture initiation 144
Curante virgin olive oil 280
Cuticle 18
Cutting types 132
Cuttings 5, 125133
Cycle length 77
Cycloartenol 28
Cytokinins 16, 130, 174, 214, 215
ethylene 215
plant stress 214
role 214
Cytoplasmic male sterility 94
Dark respiration (RD) 72
Darkness 146
Decit irrigation 163
Denitrication 185
Dense planting varieties 122
Density of planting 121
Desertication 78, 295
De-stoning 127, 271
Differential thermal analysis (DTA) 59, 61, 62
Dimethyloleuropein 28, 29
Discontinuous processing system 279
Disease control 319, 322
Disease growth 78
Disinfection 144
Distal end 129
Diuron 329, 330
DNA polymorphism 288
Dormancy 22, 57, 126, 128
Double sigmoid 22, 103
Double-bladed knife 143
Drip irrigation 161, 322
Driselase 149
Drupe 20, 22, 25, 229, 230
Dual purpose cultivars 23
Dwarng rootstocks 17, 43, 44, 114
Ecodormancy 22
Efciency of transpiration 167
Egg cell 100
Electrical conductance 68, 211
Electrical resistance blocks 68
Electrolyte release 61
Electronic leaf 133
Embryo dormancy 127
Embryo sac 22, 100
Endocarp (stone) 20, 22, 25, 26, 28, 229,
230, 235
401
Endodormancy 22
Endogenous hormones 106, 222, 224
Environmental problems 295
Epan 154
Epicarp 25, 27, 28, 230
Ethephon and ripening 230, 252
Ethrel 252
Etiolation 131, 354
European Union table olive consumption 41
European Union table olive production 40
Evapotranspiration 154
estimation methods 154, 162
Exocarp 20, 103
Explant rooting 146
Extra virgin olive oil 30, 38, 280, 281, 313
Farming systems 49
Fentons reaction 298, 299
Fermentation 164, 271273, 275, 276
aerobic 276
anaerobic 276
Fertilization 22, 52, 180, 186, 319, 321,
324, 331, 332, 345
Fertilization optimal rate 208
Field capacity 66
Flashpoint 284
Floranid 190
Florets 100
Flower bud induction 20, 22, 82
Flower distribution 97
Flower induction 81
Flower fertilization 93, 102
Flower initiation 81
Flower phenology 93
Flowering bud induction 16, 53, 54, 82
date of harvesting 90
defoliation 91
differentiation of owers 82
fruit thinning 90
growth regulators 87, 88
juvenility 85
LAS index 84
light quality 84
light shading 84
morphological changes 82
sugars natural elements 89
temperature chilling 86
variation of temperature 87
Flowering date prediction 79, 94
Flowering period 97
Fluorescence 73
402
Index
Fog system 132

Foliar application of nutrients 209
Foliar uptake 209
Frankliniella occidentalis 335, 341, 342
Frantoio 58, 126, 212, 213, 262, 263, 266,
353
Freezing point depression 69
Freezing symptoms 54
Fresh olives storage 278
Frost-hardy cultivars 63
Frost sensitivity 51
Frost-susceptible cultivars 63
Frost tolerance 57, 58, 62
calcium 62
olive varieties 62
Fruit acceleration 251
Fruit chemical composition 27
Fruit color 26, 229, 241
Fruit detachment force (FDF) 229, 245, 246,
252254
Fruit growth 22, 52, 103, 229
assimilate supply 229
Fruit load 20, 22, 105
ripening 229
Fruit ripening
olive oil quality 241
Fruit set 22, 47, 83, 100, 102, 223, 350
Fruit shrinkage 54, 55
Fruit size and ripening precocity
orchard orientation 234
tree vigor 234
Fruit structure 25
Fruit thinning 109
FS-17 122, 185, 263
Fungal diseases 346
Gaidurolia 267
Galatsaniki 260
Gas spectroscopy 287
Gathering tools 247
Gene regulated by salt stress 219, 354
Genic male sterility 94
Genotypic difference 185
Gibberellic acid 16, 22, 87, 88, 130, 146
Girdling 73, 106, 221
Glaciers 9
Global table olive consumption 40
Global table olive production 36
Global warming 76
Globe system 120
Glutamine synthetase 186
Glutamine-2 oxoglutarate transminase

(GS-GOGAT) 186
Gordal 47, 268
Gordal Sevillano 269
Graft union 138, 139
Grafting 4345, 125, 138, 139
recommended for 138
Grafting methods 140143
Grafting time 140
Grass-cutting machines 331
Greek olive cultivars 28, 258
Green maturation 229
Green ripe olives 230, 236, 272, 274
Greenhouse effect 74
Grossa di Spagna 270
Ground water 152
Growth ushes 105
Growth phases 230
Habit 13, 223

Hand-harvesting 245
Hardwood cutting 130, 132
Harvesting 227, 241, 245254, 272, 285,
322, 324, 352
Harvesting criteria 229
Harvesting nets 247
Harvesting tools 247
cylinder harvester 248
scissors-type harvester 247
tweezers-type harvester 248
Heat stress 52, 53
Heat waves 76
Hectareage of olives 33
Hemiberlesia lataniae 335, 343, 344
Herbicides 295, 324, 325333
absorption 325, 326
biological control 333
contact 325, 329, 330
hormonal action 325, 330
orchard age 327329
post-emergence 325, 328331
pre-emergence 325, 328330
High-density system 47, 50, 121
appropriate varieties 122
History of olive 1
Hurricanes 76
Hydraulic conductivity 67
Hydraulic resistance 158
Hydrogen production 301
Hydroxytyrosol 2931, 239, 241, 298, 299,
303, 305, 311313, 315, 317
Hydroxytyrosol levels 234
Index
IAA production 130, 222224
IBA concentration 137
IBA solution 132, 133, 146
Imperfect ower 84, 183
In vitro culture 143150
micropropagation aims 143, 144
Inactivation of cytochrome P450 305
Incompatibility 43, 138
Indirect organogenesis 144
Inltration 153
Infrared thermography 161
Inorganic N sources 146
Insects 335346
Integrated management 323, 324
Integrated olive culture 319, 323, 324
Integrated weed management 331, 333
Internal CO2 (Cj) 72
Inter-simple Sequence repeats ((ISSR) 289
Iron 199
phosphorous solubility 199
Iron imbalance 199
Irrigation 15, 151166, 183, 186, 211, 241,
319, 323, 324, 345, 327, 328, 330,
350
Irrigation methods 28, 156
Irrigation need 156
root system 156
Irrigation programming methods 160
Irrigation quality 164
Juvenile cuttings 131, 133

Juvenility 129
K+-Na+ antagonism 214
K232 164, 240
K270 164, 240, 241
Kalamon 46, 81, 138, 185, 204, 205, 213,
219, 261, 262, 267, 271273, 275,
290, 353
Karydolia 267, 268
King David 5
Km 191, 192
Kolovi 87, 262
Konservolia 267
Koroneiki 46, 47, 54, 58, 81, 87, 106, 122,
127, 157, 185, 186, 204, 219, 230,
246, 249, 257259, 268, 290
Kothreiki 219, 261, 290
Ladolia 256
Lag phase 190
Laminar air ow 146, 147

Lampante virgin olive oil 280
Large-fruited olive cultivars 267270
Latent buds 19, 137
Leaf abscission 16, 58, 71, 346, 352
Leaf characteristics 18, 255
Leafy cuttings 43, 45, 129, 132
Leaf fossils 1
Leaf analysis 181, 324
Leaf orientation 53
Leaf rolling 69
Leaf sampling season 181
Leaf surface characteristics 53
Leaf water potential (leaf) 160, 172
Leaf : fruit ratio 110, 229
Lianolia Kerkiras 219, 259
Lianolia Patron 260
Light interception 71
Lineweaver Burk plot 191
Linolenic acid 236
Liquid nitrogen 150
Local cultivars worldwide 257
Loosening agents 252
Low chilling requirements 51, 356
Low temperature stress 54
Low vigor cultivars 114, 122
Low volume irrigation 161
Low-frequency decit irrigation 164
Luteoxanthin 238
Magnesium 198, 199

Main olive cultivars 258
Malondialdehyde (MDA) 216
Manganese 199
Manganese deciency 199
Manganese form 200
Mannitol 25, 54, 146, 175, 176, 216
families containing 216
Mannitol accumulation 54, 175
Mannitol content 177, 216
Mannitol degradation 176
Manzanillo 17, 4446, 106, 157, 205,
262264, 268, 271273, 276
genotypes 265
Mastoidis 81, 157, 204, 260, 290
Matric potential (m) 54, 65
Maturation stage evaluation 242
Maturity index (MI) 243
Mechanical harvesters 248, 249, 253
over-row harvester 124, 249
tree characteristics 251
403
404
Index
Mechanical harvesting efciency 253

orchard characteristics 251
Mechanical pruning 226
Mechanical scarication 127
Mediterranean diet 316, 317
ageing 316
cancer prevention 317
minor components 317
Medium-fruited olive cultivars 260267
Meuidine 62
Megaritiki 46, 58, 81, 157, 204, 219, 260,
268
Meski 219, 264
Mesocarp (esh) 20, 25, 45, 103, 235
Methaemoglobinaemia 187
Methane 75
Methods to reduce growth vigor 47
Methods to solve nutritional problems 181
Micro-capsules 148
Micronutrients 199203
Micropropagation stages 144
Mineral nutrition 44, 105, 130, 179
Mineral requirements 180
Minoan civilization 2, 4
Mission 17, 4446, 264, 271, 273
Mist system 132. 133
Mixed shoots 17
Modied Penman equation 154
Moisture
effect on callus 139
equivalent 66
Molecular markers 255, 288, 353
Monocone system 118, 253
Morphology and physiology of olive 22
Morphology of olive 13
Mount layering 130, 131
MS 145
Mycenaean civilization 4
Mycocentrospora cladiosporioides 335, 350, 351
15N-labeled urea 209

N, P, K and Ca requirements 179, 180
N : P ratio 185
NAA as thinning agent 112
NaOH treatment 30, 128, 276
Neolithic period 5
Net assimilation (Pn) 71
Net assimilation rate 168
Neutron probe 68
Nitrate absorption 179, 188, 190194
accompanying cations 193
calcium 192
kinetics 190194
light intensity 194
nitrogen deciency 193
pH 192
temperature 192
Nitrate accumulation 187
Nitrate assimilation 186
Nitrate concentration and absorption rate 190
Nitrate leaching 187189
Nitrate reductase (NR) 173, 174, 186, 190
Nitrate reduction 196
Nitrate transport systems 194
Nitrication 184
inhibition 188
Nitrogen 16, 20, 22, 52, 102, 179296, 215
excess and olive oil 186
translocation in soils 184
Nitrogen assimilation 179, 95, 195, 196
Nitrogen fertilization time 183
Nitrogen fertilizers 183, 184
Nitrogen form and growth 194
Nitrogen mobilization 185
Nitrogen and photosynthesis 185
Nitrogen and polyphenols 186
Nitrogen quantity 183
Nitrogen soil forms 184
Nitrous oxide 74
Non conventional methods 288, 289
Non-saponiable components of virgin
olive oil 236
N-serve 188
Nuclear Magnetic Resonance (NMR) 287
Number of olives 33, 34
Nutrient
concentration in olive fruits 204
concentration with the various plant
components 181
waste water 166
water use efciency 169
Nutrient deciency 181, 182, 185
Nutrient excess 182, 185
Nutrient sufciency 181, 182
Ocean acidication 76
o-diphenol 240
Off year 87
Oil bottling 281
Oil categories 280, 281
Oil stability 165
Oil storage 281
Olea 9, 12, 14, 43
Olea chrysophylla 10, 17, 43, 114, 127
Index
Olea europaea 5, 9, 14, 29, 30, 43, 57, 138,
255, 312
Olea excelsa 10
Olea noti 1
Olea oblonga 17, 4346, 114, 349
Olea oleaster 10, 14
Olea sativa 10, 14, 113, 139, 141
Olea sylvestris 127
Olea verrucosa 43, 114, 127
Oleic acid 122, 212, 236, 241, 285, 307,
309311, 356
antiongogenic action 307, 310
Oleuropein 2831, 89, 90, 235237, 241,
242, 272, 298, 299, 303305, 312,
315, 317
antimicrobial activity 303, 304
antimycoplasmal activity 315
antioxidant 303
atherogenetic disease 304
Oleuropein biosynthesis 29
Oleuropein degradation 29
Olive leaf extract 303306
anti-HIV activity 306
Olive Medium (OM) 145, 146
Olive mill by-products utilization 296
Olive mill products 289, 295
Olive mill waste water 296301
characteristics 300
Olive oil
Alzheimers disease 310
breast cancer 307
cardiovascular diseases 309
chlorophyll a and b 237, 233
colonic cancer 308
diabetes 310
endometrial cancer 308
human cancer 307
minor constituents 242
obesity 309
ovarian cancer 308
stone removal 242
Olive oil certication 282
Olive oil colour 285
Olive oil exports 38
Olive oil extraction systems 279, 295
Olive oil avor 242, 282, 285, 311
Olive oil pollution 285
Olive oil standards 282
Olive oil synthesis 229, 236
Olive oil varieties 255
Olive oil world production 34
Olive oils characteristics 280
Olive pomace 296, 297, 301
405
Olive products 271, 274

Olive stone 125, 126
Olive stone germination 53
Olives darkened by oxidation 274
Olivir 305, 306
Olympic games 2
On year 87
Optimum temperature 52, 53, 347, 351
Organic acid
composition of virgin olive oil 235
olive oil 235
Organic cultivation 319
Organic farming 78
Organic matter 63, 188, 189, 295, 296, 297,
300, 322, 326
Organic olive orchards 189
Organogenesis 143, 144
Origin of olive 9
Osmoprotective function 175
Osmoregulatory capacity 156, 159
Osmotic potential (s) 65, 212
Osmotin 57, 58, 354
Ovary size 101
Ovule 20, 22, 101, 125
Oxidative stability (K225) 164
Oxyenus Maxwelli 345
Ozone 70, 354
Packing and labeling 277

Palmette system 120
Palpita unionalis 341
Panicle 83
Parenchymal cells 129
Parlatoria oleae 260, 264, 270, 342, 343
Partial root-zone drying 163, 170172
Patch budding 142, 143
season 142
Peat moss 134
Peltate trichome 18
Perfect ower 20, 22, 8184, 100, 183
Perlite 134, 135
Perlite properties 135
Permanent wilting percentage (PWP) 66
Permitted phytoprotective materials 320
Peroxide index 240, 241, 284
Pest control 50, 319, 320, 322, 335, 339
Pharmaceutical capsules 149
Phenolic acids 106
Phenolic compound 28, 30, 241, 242, 311,
312, 317
anticancer potential 311
olive mill waste water 299, 315
406
Phenols 28, 235, 239, 241, 242, 279, 297,
312, 313, 315
Phloeotribus scarabeoides 344, 345
Phoenician civilization 4
Phosphate fertilization 198
Phosphorous 166, 185, 197, 198
Phosphorous deciency 185
Phosphorous inorganic soil 198
Phosphorous ionic form 198
Phosphorous organic soil 198
Phosphorous roles 197
Photo-oxidation 298
Photosynthetic active radiation (PAR) 71
Phytouene 238
Phytoprotective materials 320
Pistil receptivity 94
Piston ow 188
Plant material choice 130
Plantation system 113, 114, 116
intensive 116
organic 117
semi-intensive 116
traditional 116
Planting 44, 113124, 319, 325, 327, 328
Planting date 137
Planting distances 114, 116, 137
Planting season 131, 133
Planting systems 44, 113, 324
Plastic nets 248, 322
Pleistocene period 9
Pn 156, 157, 204, 212, 213
Pneumatic beating poles 246248
linear poles 246
T-shaped poles 246, 247
Polarity 129
graft union 140
Pollen 1, 13, 53, 94100
antioxidative enzymes 94
Pollen dissemination 98
Pollen germination 97, 100
Pollen incompatibility 102
Pollen viability 96
Pollination 22, 51, 54, 83, 93102, 111,
230
Pollinators 96, 353
Polyconic system 118
Polygamous 84
Polyphenol content 164, 356
Polyphenol levels 233
Pomace olive oil 30, 281, 284, 286, 295
solvent extraction 297
Pore space 63
Index
Poseidon 2
Potassium 52, 166, 196, 197, 321
drought tolerance 208
Potassium-exchangeable 197
Potassium needs 197
Potassium-readily available 197
Potassium roles 196
Potassium-soil forms 197
Prays oleae 225, 270, 320, 322, 335, 339,
340, 355
Preformed roots 128
Pressure potential (p) 65
Processed olive types 273
Processing equipments 273
Proliferation media 147
Proline accumulation 175, 219
Proline biosynthesis, enzymes 176
Proline synthesis 175, 176
Propagation 125150
Protected Designation of Origin (PDO) 282,
289
Protected Geographical Indication (PGI) 283,
289
Protection of human erythrocytes from
oxidative damage 313, 314
Protein denaturation 29
Protoplasts 149
Proximal end 129, 140
Pruning cost 222, 224, 225
Pruning effects
ower bud induction 223
hormones 224
photosynthesis 223
shading 223
shoot length 224
Pruning frequency 227
Pruning intensity 222, 227
Pruning late 227
Pruning machinery 226
Pruning mature trees 225
Pruning objectives 221, 225
Pruning rules 222
Pruning season 227
Pruning shapes 226
Pruning techniques 221
Pruning tools 225
Pruning young trees 225
Pseudomonas syringae pv. savastanoi 122, 224,
259, 264, 265, 267, 269, 322, 335,
351, 352
Pungency 241
Purity standards 283
Index
Q10 quotient 192
Radio waves-advantages 216

Random Amplied Polymorphic DNA (RAPD)
256, 264, 288, 290
RAPD markers 45, 256, 288
Raw olives 28, 272
processing 273
Raw olives storage 272
Reactive nitrogen species 314, 315
Reactive oxygen species (ROS) 203, 216,
312314
Reducing sugars 233
Rened olive oil 281, 284, 312
Rened pomace oil 281
Regulated decit irrigation 164, 170172
Rejuvenation 144, 226
Resistance to pests and diseases 255, 353,
355
Restriction Fragment Length Polymorphysm
(RFLP) markers 256
Ring budding 138
Ripening changes 240
Ripening index (RI) 243
Ripening period 23
Root grafts 141
Root length 52, 156
Root as storage tissue 16
Rooted cutting 16, 43, 133
Rooting media 147
Rooting percentage 137
Rooting substrate 132
Rootstock 1517, 4345, 139, 140, 349,
350, 355
Rootstock description 45
Allegra 45
cultivars 45, 46
Olea oblonga 45
Wild olive 43, 45, 46
Rootstock roles 44
fruit size 44
salt tolerance 44
tree size 44
Rootstock selection 113
Rootstock vigour 46
Rubisco 72
Rubra 54
Saddle grafting 138

Saissetia oleae 225, 260, 264, 266, 267, 269,
270, 322, 335, 338, 339
407
Salinity 43, 44, 211220, 345

cytokinin metabolism 215
cytokinin transport 215
mannitol content 216
oxidative stress 216
phenols 212
plant growth 211
water absorption 212
Salinization and pollen viability 211
Saloneque 266
Salt accumulation 16, 152
Salt semi-tolerant cultivars 219
Salt sensitive cultivars 219, 213
Salt tolerance 44, 211, 214, 217, 354, 355
cultivars 219
genes 219, 220, 354
mechanism 219
potassium levels 216, 219
radio waves 216
SOS1, SOS2, SOS3 214, 219, 354
Sandy loam 52, 64
Santa Caterina 270
Santo Agostino 270
Sap ow 157, 161
Sap pH 173
Saturated ow 67
Sea level rising 76
Secondary processing 271, 277
Seed coat dormancy 127
Seed collection 126
Seed dormancy 127
Seed propagation 125
Seed storage 126
Seedbeds 128
Seedlings 16, 17, 4345, 125, 128, 141
Self-breaking beds 149
Self-pollination 99
Self-sterility 94
Semi-hardwood cutting 132
Sensing low temperature 57
Sensory evaluation 281, 282
Sequence Characterized Regions (SCAR) 288
Sevillano 17, 4446, 51, 268, 271273, 276
Shield budding 142
Shikimic acid 107
Shoot bearing shoots 17
Shoot elongation 144, 146
Shoot proliferation 144, 145
Shriveled black olives 274
Side veneer grafting 138
Sigmasterol 238
Simazine 330
Simple Sequence Repeats (SSR) 288, 290
408
Index
Single Nucleotide Polymorphism (SNP) 256,

289, 290
Slow release-fertilizers 189, 190
Small-fruited olive cultivars 256260
Sodium alginate 148
Sodium hypochlorite 144
Sodium translocation 213
Softwood cutting 132
Soil aggregates 64, 326
Soil condition 15, 16, 51, 63, 255, 285, 324
Soil cultivation 321, 322, 324, 331, 333
Soil fertility spacial variability 208
Soil horizons 65
Soil particles 64
Soil pH 52
Soil porosity 64
Soil properties 16, 63, 326
Soil structure 64
Soil texture 64, 326
Soil water measurement 67
direct method 67
indirect method 68
Soil water readily available 66
Somatic embryogenesis 144, 147, 148
Sowing 128
Spanish-type green olives 275
Specic Absorption Rate (SAR) 152, 153
Specic ion toxicity 153
Sperm cells 100
Sphaeroblast 15, 130, 137
Spilocaea oleaginea 335, 346348, 350, 355
Splice grafting 142
Split olives 275
Split root system 156, 163, 167
Spring vegetative wave 22
Squalene 236, 242, 311, 317
cancer protection 311
Squalene concentration 286
SSR markers 256
Staminate ower 20, 81, 96
Stem water potential 160
Sterols 28, 235, 238, 287
Stock plants 144
Stomata 18, 20, 167, 223, 326, 351
Stomatal conductance (Gs) 18, 71, 156158,
186,
Stomatal mechanism 152
Streptomycin sulfate 144
Subculturing 145
Suberin 128
Suckers 125, 137, 138
Sugars of olive fruits 235, 236
Sulfur dioxide 70
Sulfuric acid treatment 127

Supercooling 54, 61
Super-high-density orchards 123, 222, 249
Superoxide dismutase (SOD) 95, 354
Sustained decit irrigation 164
Synthetic seeds 144, 148
Table olives
characteristics 243, 244
most important varieties 271
ripening criteria 244
Table olives quality 278
Tanche 266
Taxonomy of olive 11, 13
T-budding 142
Temperature
effect on callus 139
fruit set 102
maximum 51
minimum 51
Temperature requirements 51
Temperature stress 52, 54
Tensiometry 68
Thasitiki 261
Thermal conductivity 68
Thermal requirements 53
Thermocouple psychometry 69
Thidiazuron 146, 148, 215
Thinning methods 110, 111, 112
chemical thinning 111
hand thinning 110
mechanical thinning 111
Tissue oxidation 144
Tocopherol concentrations of certain oils 239
Tocopherols 186, 235, 238, 239
-tocopherol 30, 165, 242, 317
Tolerance
abiotic stress 353, 354
heat stress 52
salt 204, 353
sea water 205
water stress 52, 168, 353
Tongue grafting 138
Toxic ions in waste water 166
Training 113, 117, 221
Transpiration 133, 151, 168
Transpiration quotient 151, 168
Transpiration rate 72
Tree crown 137, 349, 350
Triglycerids 236
Triterpenoids 28
Trunk diameter variation 160
Index
Trunk girth 156
Trunk shakers efciency 253
Trunk shrinkage 160
Tsunati 259
Tyrosol 2830, 239, 241, 312, 313, 315
Ultraviolet (UV) absorption 286

Ultraviolet (UV) light absorbance 284
Ultraviolet (UV) spectroscopy 287
Vase system 117

Vasebush system 119
Vegetative buds 17, 22, 58
Vegetative shoots 17
Verdale 266
Vermiculite 134, 135
Vernalization 51
Verticillium dahliae 45, 122, 225, 260, 263,
264, 266, 335, 348, 349, 355
Vibration characteristics 251
Viral diseases 352
Virgin olive oil 30, 31, 38, 241, 280, 281,
283, 289, 295, 312, 314, 316, 317
Vmax 191, 192
Wagga Verdale 266

Waste water 30, 31, 165, 166, 297
extraction and their hypocholesterolaemic
effects 315
use as fertilizer 299
Water application scheduling 160
Water content 151
Water criteria 153
Water cured olives 275
Water decit avoidance 167
Water inltration 67
Water logging 67, 69
Water potential (soil) 65, 156, 157, 212
409
Water quality 152, 153

Water requirements 152, 153
Water sprouts 17, 225, 227
Water stress 43, 52, 58, 100, 101, 130, 164,
175, 180, 215, 227, 259, 325, 354
critical period 162
Water use efciency (WUE) 73, 156, 157,
163, 167174, 223
denition 168
Water vapor 74
Waxing 141
Wedge grafting 138, 142
Weeds 134, 322, 323, 325333
common names 327
scientic names 327
sensitivity to current herbicides 332
Wetting zone 67
What is pruning 221
Whip grafting 141
Why to prune 221, 222
Wilting 66
Wind-affected areas 52, 324
Wound roots 128
Wounding 131
WPM 145
Wrapping 143
Wreath 2
WUE
methods to improve 170172
regulated decit irrigation 170, 171
WUE measurement 169
WUEe 168
WUEt (transpiration efciency) 168
Xylopode 15
Zeatin 146, 172, 174

Zeuzera pyrina 345, 346
Zygote 100

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CROP PRODUCTION SCIENCE IN HORTICULTURE

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CABI is a trading name of CAB International

Tel: +1 617 395 4056

Ioannis Therios 2009. All rights reserved. No part of this

ACRONYMS AND ABBREVIATIONS

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Olive culture is rapidly growing in terms of both production volume and

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ACRONYMS AND ABBREVIATIONS

Acronyms and Abbreviations

Acronyms and Abbreviations

Acronyms and Abbreviations

Partial rootzone drying

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The Olive Tree

History of Olive Growing

of olive orchards. Archaeological studies supply circumstantial evidence for

History of Olive Growing

History of Olive Growing

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species, such as O. chrysophilla and O. excelsa, probably participated in the

Countries with olive cultivation and the botanical species

Origin and Classication

Table 2.2. Botanical species of Olea, by continent.

USA: Florida, Georgia, Carolina, Virginia

CLASSICAL TAXONOMY OF THE CULTIVATED OLIVE

Order: Scrophulariales or Lamiales

According to De Candolle the genus Olea includes Gymnelaea and Euelaea,

sativa (cultivated olive)

The olive tree is long-lived, with an average height of 1520 m. However,

Morphology and Taxonomy

Table 3.1. Various species of olive, by continent.

ANATOMY OF THE OLIVE TREE

Anchorage of the tree.

Fungi, bacteria and nematodes reduce root growth. Adequate

Morphology and Taxonomy

irrigation water reduces root growth. Concentrations considered to be toxic are

Morphology and Taxonomy

Table 3.2. The main characteristics of olive leaves.

stage there is a transition stage, which is followed by increasing internode

Inorescences and owers

Morphology and Taxonomy

GROWTH, MORPHOLOGY AND PHYSIOLOGY OF THE

Morphology and Taxonomy

in the middle of October, completion time depending on the cultivar.

Fig. 3.7. Ripening periods of 36 selected worldwide olive cultivars.

Structure and Composition

Table 4.2. Chemical composition of the olive fruit pulp, by weight.

Table 4.3. Composition (percentage) of CHCl3EtOH-extractable classes of

Alcohols, aldehydes and fatty acids

Structure and Composition

Oleuropein is abundant in the young olive fruits and comprises

OLEUROPEIN AS A PROTEIN DENATURANT AND AS A DEFENCE MECHANISM AGAINST

Oleuropein is a secoiridoid glycoside, which is common in the

Structure and Composition

Hectareage (ha 1000)