Escherichia coli BL21(DE3): B21_02369
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Entry
B21_02369 CDS
T02096
Symbol
ispG
Name
(GenBank) 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
KO
K03526
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC:
1.17.7.1
1.17.7.3
]
Organism
ebe
Escherichia coli BL21(DE3)
Pathway
ebe00900
Terpenoid backbone biosynthesis
ebe01100
Metabolic pathways
ebe01110
Biosynthesis of secondary metabolites
Module
ebe_M00096
C5 isoprenoid biosynthesis, non-mevalonate pathway
Brite
KEGG Orthology (KO) [BR:
ebe00001
]
09100 Metabolism
09109 Metabolism of terpenoids and polyketides
00900 Terpenoid backbone biosynthesis
B21_02369 (ispG)
Enzymes [BR:
ebe01000
]
1. Oxidoreductases
1.17 Acting on CH or CH2 groups
1.17.7 With an iron-sulfur protein as acceptor
1.17.7.1 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)
B21_02369 (ispG)
1.17.7.3 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
B21_02369 (ispG)
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Motif
Pfam:
GcpE
DeoC
Motif
Other DBs
NCBI-ProteinID:
CAQ32886
UniProt:
A0A140N4P3
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Position
complement(2505160..2506278)
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AA seq
372 aa
AA seq
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MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVR
VSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRM
VVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSV
KASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
SIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLD
EARRIDVQQVEK
NT seq
1119 nt
NT seq
+upstream
nt +downstream
nt
atgcataaccaggctccaattcaacgtagaaaatcaacacgtatttacgttgggaatgtg
ccgattggcgatggtgctcccatcgccgtacagtccatgaccaatacgcgtacgacagac
gtcgaagcaacggtcaatcaaatcaaggcgctggaacgcgttggcgctgatatcgtccgt
gtatccgtaccgacgatggacgcggcagaagcgttcaaactcatcaaacagcaggttaac
gtgccgctggtggctgacatccacttcgactatcgcattgcgctgaaagtagcggaatac
ggcgtcgattgtctgcgtattaaccctggcaatatcggtaatgaagagcgtattcgcatg
gtggttgactgtgcgcgcgataaaaacattccgatccgtattggcgttaacgccggatcg
ctggaaaaagatctgcaagaaaagtatggcgaaccgacgccgcaggcgttgctggaatct
gccatgcgtcatgttgatcatctcgatcgcctgaacttcgatcagttcaaagtcagcgtg
aaagcgtctgacgtcttcctcgctgttgagtcttatcgtttgctggcaaaacagatcgat
cagccgttgcatctggggatcaccgaagccggtggtgcgcgcagcggggcagtaaaatcc
gccattggtttaggtctgctgctgtctgaaggcatcggcgacacgctgcgcgtatcgctg
gcggccgatccggtcgaagagatcaaagtcggtttcgatattttgaaatcgctgcgtatc
cgttcgcgagggatcaacttcatcgcctgcccgacctgttcgcgtcaggaatttgatgtt
atcggtacggttaacgcgctggagcaacgcctggaagatatcatcactccgatggacgtt
tcgattatcggctgcgtggtgaatggcccaggtgaggcgctggtttctacactcggcgtc
accggcggcaacaagaaaagcggcctctatgaagatggcgtgcgcaaagaccgtctggac
aacaacgatatgatcgaccagctggaagcacgcattcgtgcgaaagccagtcagctggac
gaagcgcgtcgaattgacgttcagcaggttgaaaaataa
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