KEGG ENZYME: 2.4.1.150

ENZYME: 2.4.1.150

Entry

EC 2.4.1.150                Enzyme

Name

N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase;
GCNT2 (gene name);
GCNT3 (gene name);
IGnT;
I-branching beta1,6-N-acetylglucosaminyltransferase;
N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-acetyllactosaminide beta1->6-acetylglucosaminyltransferase;
Galbeta1->4GlcNAc-R beta1->6 N-acetylglucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide beta-1,6-N-acetyl-D-glucosaminyltransferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminide 6-beta-N-acetylglucosaminyltransferase (configuration-inverting)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [RN:R11879]

Reaction(KEGG)

R11879 > R06189(G);
(other) R02790 R04654 R06013(G)

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [CPD:C21742]

Product

UDP [CPD:C00015];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R [CPD:C21743]

Comment

The enzyme acts on poly-N-acetyllactosamine [glycan chains of beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine units connected by beta(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by beta(1,6)-linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.

History

EC 2.4.1.150 created 1984 (EC 2.4.1.164 created 1989, incorporated 2016), modified 2017

Pathway

ec00601

Glycosphingolipid biosynthesis - lacto and neolacto series

ec01100

Metabolic pathways

Orthology

K00742

N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase

Genes

HSA:	2651(GCNT2)

PTR:	100610053 462428(GCNT2)

PPS:	100979555 100983940(GCNT2)

GGO:	101133045(GCNT2) 109027295

PON:	100173016(GCNT2) 100451069

PPYG:

129037860(GCNT2)

NLE:	100600035 100601598(GCNT2)

HMH:	116460012(GCNT2)

SSYN:

129473096(GCNT2)

MCC:	697468(GCNT2)

MCF:	102131882(GCNT2)

MTHB:

126953872

MNI:	105482411(GCNT2) 105487415 105487416

CSAB:

103222182 103222183

CATY:

105572520 105572521

PANU:

101020180(GCNT2) 103883455 103883457

TGE:	112623439(GCNT2) 112623440

MLEU:

105546449 105546450

RRO:	104656690 104671974

RBB:	108521659 108521996 108522006 108522008 108528720

TFN:	117086715(GCNT2)

PTEH:

111521788(GCNT2)

CANG:

105526628(GCNT2) 105526629 105526805

CJC:	100397136 100404131 100411905 100895187

SBQ:	101030514(GCNT2) 101044056

CIMI:

108302945 108302947 108302949

ANAN:

105708655 105708730(GCNT2) 135268130

CSYR:

103257198 103265522 103265534

MMUR:

105865110 105865180 105865181(GCNT2)

LCAT:

123638228 123638229 123638230

PCOQ:

105806482 105806483 105806487 105824853 105824854

OGA:	100944503 100956694 100957010 105886840

MMU:	14538(Gcnt2)

MCAL:

110307912

MPAH:

110333783(Gcnt2)

RNO:	306860(Gcnt2)

MCOC:

116081738

ANU:	117713801 117714285 117714286(Gcnt2)

MUN:	110554603(Gcnt2)

CGE:	100760161 100763488 118238511

MAUA:

101824660(Gcnt2)

PROB:

127229309(Gcnt2)

PLEU:

114692111 114692131

MORG:

121433997(Gcnt2)

MFOT:

126501511

AAMP:

119817452 119817453(Gcnt2)

NGI:	103732899(Gcnt2) 103732948

HGL:	101701828 101702179(Gcnt2) 101715209 101715564 101724068

CPOC:

100723536(Gcnt2)

CCAN:

109700881(Gcnt2) 109700883

DORD:

105985988 105985989

DSP:

122106996

PLOP:

125353036(Gcnt2)

NCAR:

124988358

MMMA:

107149240

OCU:	100356713 103349711

OPI:	101533291(GCNT2) 118758264

TUP:	102469358 102485510(GCNT2)

GVR:	103602336(GCNT2)

CFA:	488215(GCNT2)

CLUD:

112658449(GCNT2)

VVP:	112920550 112920551

VLG:	121499590(GCNT2)

NPO:	129507811 129508007 129508008

AML:	100468405(GCNT2) 117802286

UMR:	103664926 121099730 121106075

UAH:	113264440 130542207

UAR:	123801609 123801610

ELK:	111156684 111156685 111156720

LLV:	125102205 125102206

MPUF:

101681334(GCNT2)

MNP:	132017595(GCNT2)

MLK:	131834515(GCNT2) 131834516

NVS:	122912699(GCNT2)

ORO:	101380605 101383306 101383589

EJU:	114212530(GCNT2) 114212531

ZCA:	113926599(GCNT2)

MLX:	118027214 118027450

NSU:	110586640 110586892

LWW:	102727112(GCNT2) 102728644

FCA:	101094925(GCNT2) 109491352

PYU:	121020542(GCNT2) 121020543

PCOO:

112853742 112853769

PBG:	122489246(GCNT2)

PVIV:

125165761(GCNT2) 125165762

LRUF:

124528243

PTG:	102972600(GCNT2)

PPAD:

109249650(GCNT2)

PUC:	125939572 125939574 125939575

AJU:	106972037 113592392 128315787

HHV:	120231663(GCNT2) 120231665

BTA:	112443869 520336

BOM:	102276215 102276507 102277629 102277917

BIU:

109577218

BBUB:

102401082(GCNT2)

BBIS:

104985613 104985614

CHX:

100861326

OAS:

101106554

BTAX:

128055247 128055248

CSUM:

138069474(GCNT2) 138098003

ODA:	120869900 120869903

CCAD:

122429708(GCNT2) 122429987

MREE:

136151433(GCNT2)

MBEZ:

129543892

SSC:	100152566 102157807 110255306

CFR:	102520753(GCNT2)

CBAI:

105061625 105061626

CDK:	105101211(GCNT2)

VPC:	102531012(GCNT2)

BACU:

103007744 103010651(GCNT2)

BMUS:

118903443 118903488

LVE:	103088671 103088938 103089209

OOR:	101282851(GCNT2)

DLE:	111181352(GCNT2) 111181354

PCAD:

102980967 129391364 129391487 129391488

PSIU:

116761802(GCNT2) 116761803

NASI:

112401361(GCNT2)

ECB:

100063291

EPZ:	103559596 103559598(GCNT2)

EAI:	106833434(GCNT2)

MYB:	102245671 102245970(GCNT2)

MYD:	102770362(GCNT2) 102770649 102770938

MMYO:

118675060(GCNT2) 118675062

MLF:	102426091 111823016

MDT:	132230720(GCNT2) 132230722

PKL:	118724427 118724428

EFUS:

103290189 114233195 129147099

MNA:	107530399 107530416 107530417

DRO:	112297544(GCNT2)

SHON:

118986439(GCNT2)

AJM:	119034524 119034528 119034529

PDIC:

114496371 118500744

PHAS:

123820762 123820763

MMF:	118627206(GCNT2) 118627207

PPAM:

129085535(GCNT2)

HAI:	109377537(GCNT2)

RFQ:	117026868(GCNT2)

PALE:

102877728 102898828

PGIG:

120598505 120598937(GCNT2) 120598938

PVP:	105300842 105300843

RAY:	107497201 107497202 107497203

MJV:	108396557(GCNT2)

TOD:	119251600 119251601 119253232

SARA:

101541384 101542179

SETR:

125996031 125996248

LAV:	100662133 100674841 100675128 100675407 100675689

TMU:	101352763 101355412 101355931 101356187 101356441

ETF:	101638403 101639346 101647352(GCNT2)

DNM:	101424024(GCNT2) 111760113

MDO:	100027080(GCNT2) 100027097 100027131

GAS:	123231849 123254659 123254681

SHR:	100925756 116420643 116420653

AFZ:	127544181 127544182

PCW:	110191997 110192005 110224059 110224060 110224061

TVP:	118835391 118835400 118858206

PBRV:

138173418 138173493

OAA:

100084006

GGA:	428479(GCNT2)

PCOC:

116226449 116226450 116241361

MGP:	100539032(GCNT2)

CJO:	107309590(GCNT2)

TPAI:

128087839(GCNT2)

LMUT:

125691366(GCNT2)

NMEL:

110394520 110394521

APLA:

101795771 119715798

ACYG:

106034848 106041798

CATA:

118254768 118254770

AFUL:

116485591 116485592

TGU:	115493561(GCNT2)

SCAN:

115483999(GCNT2)

PMOA:

120503483(GCNT2)

OTC:	121340411(GCNT2)

PRUF:

121357761(GCNT2)

GFR:	106631903(GCNT2)

FAB:	107603337(GCNT2)

OMA:	130248802(GCNT2)

PMAJ:

107215848(GCNT2)

CCAE:

111923931(GCNT2)

CCW:	109145064(GCNT2)

CBRC:

103613172(GCNT2)

ACOE:

138105186(GCNT2)

ZAB:	102074701(GCNT2)

ZLE:	135442917(GCNT2)

SVG:	106853570(GCNT2)

MMEA:

130576084(GCNT2)

HRT:	120748899(GCNT2)

SATI:

136375372(GCNT2)

FPG:	101918293(GCNT2)

FCH:	102057507(GCNT2)

CCRI:

104155881 104166264

NNT:	104405472(GCNT2)

SHAB:

115605521 115605568

ACUN:

113476690(GCNT2)

TALA:

104363045(GCNT2)

ACHC:

115352964(GCNT2)

HLE:	104835362(GCNT2)

AGEN:

126048702

HHAR:

128135690(GCNT2)

GCL:	127012660 127012661

CSTI:

104562480(GCNT2)

LDI:	104351645(GCNT2)

MNB:	103773478(GCNT2)

AVIT:

104276295(GCNT2)

BRHI:

104499582(GCNT2)

EGZ:	104127709(GCNT2) 104133970

NNI:	104021895 104021896

PADL:

103919086(GCNT2)

AFOR:

103894363(GCNT2)

CLV:	102086182(GCNT2)

MUI:	104543602(GCNT2)

PGUU:

104468166(GCNT2)

EHS:	104505168 104515194

CMAC:

104474199(GCNT2)

CUCA:

104062151(GCNT2)

TEO:

104371450

OHA:	104326807(GCNT2)

ACAR:

104527893(GCNT2)

CPEA:

104391656(GCNT2)

CVF:	104285716 104286796

RTD:	128906091(GCNT2)

AAM:	106482558(GCNT2)

AROW:

112965365(GCNT2)

NPD:	112947304(GCNT2)

TGT:	104566593 104567614(GCNT2)

DNE:	112991986(GCNT2)

SCAM:

104146145(GCNT2)

ASN:	102373136 102374392 106722091

AMJ:	102575341(GCNT2)

GGN:	109298522(GCNT2)

PSS:	102458109 102458349

CMY:	102930060(GCNT2)

CCAY:

125632129(GCNT2)

DCC:	119851432 119852310

CPIC:

101931709(GCNT2)

TST:	117871622 117873197

CABI:

116827966 116828588(GCNT2)

MRV:	120398881(GCNT2)

ACS:	100558219 100558415

ASAO:

132774585 132774586

PVT:	110080870 110080871

SUND:

121928724 121929100

PBI:

103065974

PMUR:

107282985(GCNT2)

CTIG:

120310841(GCNT2) 120315318

TSR:

106543763

PGUT:

117672478(GCNT2)

APRI:

131194433(GCNT2)

PTEX:

113445736(GCNT2)

NSS:

113430824

VKO:	123036189 123036194

PMUA:

114600911 114601371 114601373

PRAF:

128417784(GCNT2) 128417871

ZVI:	118090127 118090174 118090429(GCNT2)

HCG:	128324610 128324611

GJA:	107111763(GCNT2)

STOW:

125439305(GCNT2)

EMC:	129333096(GCNT2)

XLA:	108718735 108719711 432177(gcnt2.2.S) 432275(gcnt2.2.L)

XTR:	100038221(gcnt2.2) 100491539

NPR:	108788458 108788461

RTEM:

120940706 120940707

BBUF:

121001281 121001896

BGAR:

122938781 122939140

MUO:	115473882(GCNT2)

GSH:	117355726(GCNT2)

LOC:	102693956(gcnt2)

PSPA:

121313931

ARUT:

117394963

LCM:	102366551(GCNT2)

CMK:

103184570

RTP:

109937915

CPLA:

122564360

HOC:

132832268

LERI:

129708625

BFO:

118427322

CIN:

100175723

SPU:	115923646 578725 579029 763273

LPIC:

129269389 129269452 129269913 129269915 129269916 129275880 129276253

APLC:

110988200 110988201

ARUN:

117296454 117296455

AJC:

117100505

SKO:	100370818 100378232

TCF:	131884357 131884574 131885203 131891366

LOA:	LOAG_16642

HRO:	HELRODRAFT_139499 HELRODRAFT_163385

LGI:	LOTGIDRAFT_103000 LOTGIDRAFT_140870 LOTGIDRAFT_140905 LOTGIDRAFT_173237 LOTGIDRAFT_64655 LOTGIDRAFT_64882 LOTGIDRAFT_73453

PVUL:

126819363 126821573

PCAN:

112554326 112565138

BGT:	106058038 106058614 106060603

GAE:

121385625

HRF:

124125097

HRJ:	124253766 124269292 124270653

HASI:

137279916 137287241 137287242

CVN:

111106403

PMAX:

117318075

MMER:

123531800 123539824

RPHI:

132744760 132759814

DPOL:

127862716

MAEA:

128231703

LJP:	135476338 135476914 135477175

OVI:	T265_04703 T265_10156

LLON:

135486132 135493891 135496918

NVE:

5511223

ATEN:

116290394

ADF:

107329241

AMIL:

114974181

MCAZ:

138022797 138023331 138023381 138023397 138023558 138035502 138038894 138038895 138038946 138045815 138045873 138048864 138048865

XEN:

124440557

AQU:

105315875

» show all

Reference

1 [PMID:6219989]

Authors

van den Eijnden DH, Winterwerp H, Smeeman P, Schiphorst WE.

Title

Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.

Journal

J Biol Chem 258:3435-7 (1983)

Reference

2 [PMID:6238026]

Authors

Basu M, Basu S.

Title

Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma.

Journal

J Biol Chem 259:12557-62 (1984)

Reference

3 [PMID:6490658]

Authors

Piller F, Cartron JP, Maranduba A, Veyrieres A, Leroy Y, Fournet B

Title

Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa.

Journal

J Biol Chem 259:13385-90 (1984)
DOI:10.1016/S0021-9258(18)90706-4

Reference

4 [PMID:7579796]

Authors

Bierhuizen MF, Maemura K, Kudo S, Fukuda M

Title

Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.

Journal

Glycobiology 5:417-25 (1995)
DOI:10.1093/glycob/5.4.417

Sequence

[hsa:2651]

Reference

5 [PMID:10092606]

Authors

Ujita M, McAuliffe J, Suzuki M, Hindsgaul O, Clausen H, Fukuda MN, Fukuda M

Title

Regulation of I-branched poly-N-acetyllactosamine synthesis. Concerted actions by I-extension enzyme, I-branching enzyme, and beta1,4-galactosyltransferase I.

Journal

J Biol Chem 274:9296-304 (1999)
DOI:10.1074/jbc.274.14.9296

Reference

6 [PMID:9915862]

Authors

Yeh JC, Ong E, Fukuda M

Title

Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.

Journal

J Biol Chem 274:3215-21 (1999)
DOI:10.1074/jbc.274.5.3215

Sequence

[hsa:9245]