Entry
Name
beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase;
N-acetylglucosaminyltransferase III;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III;
uridine diphosphoacetylglucosamine-glycopeptide beta4-acetylglucosaminyltransferase III;
beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase;
GnTIII;
GlcNAc-T III;
MGAT3 (gene name);
UDP-N-acetyl-D-glucosamine:beta-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:beta-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:
R05986 ]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:
C00043 ];
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:
C00015 ];
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. The residue added by the enzyme at position 4 of the beta-linked mannose of the trimannosyl core of N-glycans is known as a bisecting GlcNAc. Unlike GlcNAc residues added to other positions, it is not extended or modified. In addition, its presence prevents the action of other branching enzymes involved in the process such as GlcNAc-T IV (EC
2.4.1.145 ) and GlcNAc-T V (EC
2.4.1.155 ), and thus increased activity of GlcNAc-T III leads to a decrease in highly branched N-glycan structures.
History
EC 2.4.1.144 created 1984, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
Pathway
Orthology
K00737 beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
Genes
OPI : 101519370(MGAT3) 101527106
CBAI : 105065538(RFNG) 105073103(MGAT3)
XLA : 108714526(mgat3.L) 108715764(mgat3.S)
DRE : 553676(mgat3a) 570828(mgat3b)
SRX : 107705910 107723596 107737986 107748818
SANH : 107666705 107672118 107676726(mgat3) 107695120
SGH : 107554701 107575428 107580620 107602307
CCAR : 109057672 109073844 109105847
CAUA : 113046809 113047697 113089912 113095183
CGIB : 127945884 127953014 127959409 128015296
PTET : 122333313(mgat3b) 122341795(mgat3a)
LROH : 127161742(mgat3a) 127166596(mgat3b)
OMC : 131537680(mgat3a) 131542218(mgat3b)
PPRM : 120462297(mgat3a) 120467959(mgat3b)
MAMB : 125273787(mgat3a) 125274429(mgat3b)
CIDE : 127509086 127513824
CERY : 137011429(mgat3b) 137017365(mgat3a)
MASI : 127446196 127449009 127450028 127452332
TROS : 130550481(mgat3b) 130561834(mgat3a)
TDW : 130424496(mgat3b) 130426574(mgat3a) 130426576
MANU : 129436480(mgat3a) 129450668(mgat3b)
IPU : 108272883(mgat3b) 108272992(mgat3a)
IFU : 128615570(mgat3a) 128616366(mgat3b)
PHYP : 113524085 113538382(mgat3)
SMEO : 124381865(mgat3a) 124383183(mgat3b)
TFD : 113634570(mgat3b) 113650583(mgat3a)
TVC : 132842570(mgat3a) 132844900(mgat3b)
TRN : 134309258 134316528(mgat3b) 134334517
AMEX : 103038268(mgat3b) 125797534 125799358
CMAO : 118805359 118807883(mgat3b)
EEE : 113580225(mgat3) 113591397
CHAR : 105890684(mgat3a) 105893539(mgat3b)
ELY : 117255410(mgat3b) 117256017
PRET : 103461029 103478406(mgat3)
BPEC : 110156010 110157727(mgat3)
SASA : 106590617 106590738 106600880 106600973
STRU : 115147444 115170934 115170935 115177729
OTW : 112236407(mgat3b) 112258491 112258492
OMY : 110485598 110495067 110538027 110538028
OGO : 123998662 124000490 124028039 124028109
ONE : 115110123 115110124 115116044
OKI : 109879001 109885480 109892212 109893205
OKE : 118380849(mgat3b) 118384381 118384383
SALP : 111979930 111981819 112078815
SNH : 120033058(mgat3b) 120043968 120043969
CCLU : 121551725 121555850(mgat3b) 121569723(mgat3a)
ELS : 105029655 105031194(mgat3)
SFM : 108928829(mgat3) 108930685
PKI : 111835978(mgat3) 111845193
AANG : 118216814 118218243(mgat3b)
ARUT : 117432620 117432850
LCM : 102355106(MGAT3) 102367673
DSI : Dsimw501_GD22083(Dsim_GD22083)
AALB : 109421021 109428872
BCOO : 119070756 119072522 119078703
FCD : 110849183 110849923 110861800
DPX : DAPPUDRAFT_46677 DAPPUDRAFT_50861
PJA : 122260128 122260704 122265541
PCHN : 125025087 125041820
PMOO : 119581920 119582159
HAME : 121878676 121879947
PCLA : 123756805 123757616
PTRU : 123507761 123520140
HAZT : 108670982 108675622
EAF : 111694722 111700852 111711074
LSM : 121115676 121116224 121116590 121116591
DSV : 119442535 119442536 119448070 119456987 119459019 119459020 119460788 119462090
RSAN : 119374354 119374355 119385914 119387319 119394636 119400782
RMP : 119159405 119167296 119167297 119172589 119174898 119177864
DPTE : 113788387 113796574
LPOL : 106461315 106462642 106463353
HRF : 124129253 124132926 124132932 124132933 124132988
HASI : 137256632 137272876 137273364
LAK : 106156444 106160705 106180254
NVE : 5504084 5506199 5515029
ATH : AT1G12990 AT1G67880 AT2G13290 AT3G01620 AT3G27540 AT5G14480
ALY : 9309738 9313126 9319864 9320338 9324696 9326042
CRB : 17882734 17887415 17891165 17893152 17895251 17898075
CSAT : 104701406 104705722 104710595 104719414 104735466 104739956 104747265 104750600 104755579 104763729 104766619 104769685 104771942 104773410 104779999 104787812 104790409 104793814
EUS : EUTSA_v10004374mg EUTSA_v10007850mg EUTSA_v10013782mg EUTSA_v10018686mg EUTSA_v10020899mg EUTSA_v10022755mg
BRP : 103831003 103836130 103837643 103846480 103850931 103856081 103870995 103871967 103875208
BNA : 106346453 106349248 106362059 106364623 106366358 106372100 106378797 106382365 106401471 106402614 106411054 106411396 106416465 106418685 106431629 106434305 125587613
BOE : 106293780 106295528 106296599 106301361 106312548 106312974 106313588 106325441 106329245
RSZ : 108809070 108814790 108818213 108823388 108837157 108839342 108855704
THJ : 104805282 104805363 104808968 104821093 104823953 104825562 104825665
CPAP : 110815727 110816842 110817169
CIT : 102613133 102616219 102617379
CIC : CICLE_v10008737mg CICLE_v10015565mg CICLE_v10031761mg
PVY : 116118308 116118552 116130328
MINC : 123195193 123195436 123213828 123217182 123222401
TCC : 18588798 18600852 18609574
GRA : 105764296 105765967 105780087 105780677 105785822 105798368
GHI : 107892132 107892246 107897903 107903735 107916613 107922586 107925985 107928696 107933179 107946245 107953685 107959924
GAB : 108454904 108458444 108463372 108465801 108476516 108482958
HSYR : 120123614 120125088 120148960 120171002 120176902 120182630 120184282 120198049 120201107 120203694 120210885 120211540 120212886 120215293 120216084
DZI : 111282529 111286196 111287868 111293045 111299560 111316615 111317910
EGR : 104414136 104422652 104423908 104423910 104436815
GMX : 100778832 100780085 100787062 100804627 100805850 100807933 100811451 100816069
GSJ : 114374030 114382333 114391447 114396641 114398431 114400882 114413078 114421839
PVU : 137807554 137812367 137815188 137836655
VRA : 106755326 106769139 106769319 106770424
VAR : 108323773 108323892 108327876 108344642
VUN : 114175147 114177842 114181063 114185868
VUM : 124828201 124828350 124841874 124847621
CCAJ : 109788914 109793227 109806861 109815579 109817247
APRC : 113847555 113849717 113858239 113867445 113872575
MTR : 11425546 11441234 25497910 25501857
TPRA : 123881942 123892578 123915777 123917019
CAM : 101497971 101501638 101506317 101513285
PSAT : 127073521 127073522 127079678 127100931 127128098
VVO : 131603265 131611407 131633342 131633383 131638436 131641150 131661120
LJA : 130713558 130715243 130729587 130739175
ADU : 107461722 107481825 107489999 107494908
AIP : 107605091 107631692 107640254
AHF : 112697289 112701793 112723299 112734357 112751431 112755189 112792180 112801920
LANG : 109338261 109345734 109347258 109350665 109358807 109362501 109362502
PCIN : 129303607 129306961 129307939 129308111 129320886
QSA : O6P43_002404 O6P43_004702 O6P43_008889 O6P43_023164 O6P43_030506
FVE : 101301624 101308596 101312728 105349597
AANS : 126787400 126790427 126799431
RCN : 112182328 112184351 112189037 112190256 112197347
PPER : 18770605 18781737 18792252
PMUM : 103321840 103329785 103329786 103341105
PAVI : 110752626 110756781 110773367
PDUL : 117622738 117635778 117637848
MDM : 103403060 103404260 103421602 103431468
MSYL : 126583467 126597586 126603410 126607990 126612370
PXB : 103949813 103959715 103960976
ZJU : 107415318 107428371 107428517
MNT : 21388864 21403473 21410331
CSAV : 115701115 115712148 115724851 115725371
CSV : 101216914 101220682 101222690 116401630
CMO : 103487429 103488964 103498114
BHJ : 120071241 120080550 120081333
MCHA : 111006592 111014573 111019712
CMAX : 111466425 111469297 111482645 111485214 111487598 111489375
CMOS : 111430084 111441246 111445612 111449715 111458568 111461467
CPEP : 111778753 111779818 111792190 111801366 111809392 111809899
RCU : 8270200 8279094 8283455
MEAN : 126656357 126665378 126675465
JCU : 105633817 105641302 105644700
HBR : 110635648 110639534 110647756 110648531 110673072
MESC : 110599928 110601042 110605511 110617717 110628536 110630193
POP : 18095324 18099929 7459880 7460749 7469042
PEU : 105109947 105121031 105122630 105132329 105138098 105140210
PALZ : 118032578 118038127 118045386 118050468 118052095
PNZ : 133671429 133674900 133688137 133697861 133705867
JRE : 108982501 108989740 108991515 108995581 109010179 109015655 118343947
CILL : 122302656 122305159 122306866 122308088 122310950 122313846
CAVE : 132165702 132179205 132180915
QSU : 112012308 112012597 112027583
QLO : 115976569 115977094 115982446
TWL : 119990629 119996258 119999512 120000146 120007957 120009514 120010610
VVI : 100254142 100254389 100259362
VRI : 117917807 117924491 117931405
SLY : 101244199 101251860 101260885 101268683 138348499
SPEN : 107005700 107011941 107020897 107024347
SOT : 102580443 102580971 102582440 102583339 102599985
SSTN : 125847951 125857084 125862055 125867944 125876970
SDUL : 129873470 129880395 129886126 129891400 129896767
CANN : 107848203 107860690 107870753 107877746 124890100
LBB : 132603149 132614011 132616941 132621297 132641150
NTA : 107759123 107769460 107772165 107779360 107798222 107799192 107809064 107811308 107815955
NSY : 104221900 104245226 104246738 104248771 104249027
NTO : 104096764 104100978 104101948 104105408 104113785
NAU : 109214985 109217075 109219177 109229656 109243565
INI : 109157469 109157994 109160247 109172075 109178803 109180587
ITR : 116004677 116011728 116012684 116025660 116027676
SIND : 105156648 105162132 105168318 105176312 105179857
OEU : 111369314 111378069 111396162 111398313 111399363 111403379 111404797 111406310 111409303
EGT : 105960685 105961016 105965530 105969747 105974775
SSPL : 121748679 121748680 121748681 121748685 121748686 121748897 121750916 121751037 121754272 121757032 121768741 121771807 121773687 121776430 121783021 121797129 121798511 121805223 121805455 121806544 121809580 121810012
SMIL : 130987537 130988258 130988261 130995507 131008704 131025341
SHIS : 125186009 125186402 125190766 125193182 125196847 125204361 125207337 125211445 125214197 125214413 125217078
APAN : 127240429 127241442 127244540 127264222 127266394
HAN : 110865126 110867343 110871646 110878366 110886733 110895082 110911252 110921502 110921688 110926375 110940330
ECAD : 122579395 122589386 122592721 122595813 122595980 122606131 122609887 122609980
LSV : 111876458 111878245 111881590 111881917 111883058 111884274 111891611 111916948
CCAV : 112501467 112507383 112513654 112515349 112515716 112516107 112525195 112528289
DCR : 108196816 108197076 108199261 108202906 108206266 108227317 108227882
CSIN : 114258155 114297999 114312575 114312678 114316264
RVL : 131298815 131304278 131310829 131312141 131320253
AEW : 130754370 130772491 130773165 130777882 130779389 130779899 130781156 130785137 130790463
BVG : 104896767 104897297 104901992
SOE : 110792262 110793238 110805627
CQI : 110695611 110704008 110707978 110709383 110712611 110725604
ATRI : 130807883 130820956 130821160 130828203
MOF : 131144261 131157344 131168421
NNU : 104591508 104593072 104598290
MING : 122069356 122077556 122078021 122078186
TSS : 122653181 122654559 122662704 122671921
PSOM : 113276161 113282314 113298238 113312623 113312637 113324122 113324128 113341904
OSA : 4329853 4329854 4336162 4352772
OBR : 102700049 102714888 102715176 102720384
OGL : 127756506 127762132 127772013
BDI : 100828716 100829334 100843118
ATS : 109735745 109736375 109758585 109773322 109781560
TDC : 119299051 119307374 119315109 119316974 119320408 119320443 119355480 119364617
TAES : 123045758 123053641 123102207 123110378 123119400 123127587 123133596 123137323 123141025 123143451 123144690 123189530
TUA : 125507788 125513544 125516035 125528665 125538717
LPER : 127298669 127299391 127335263
LRD : 124654474 124655080 124656111 124656263 124674981 124676541 124692720 124692726
SBI : 8056471 8058717 8084386
ZMA : 100194350 100282445 100282765 100501240
MFLO : 136462313 136467236 136477617 136490796 136505447 136508845
SITA : 101752767 101755522 101771384
SVS : 117850847 117860062 117864309
PVIR : 120641253 120656295 120666910 120682151 120700115 120707212
PHAI : 112888016 112889217 112899789
PDA : 103695946 103717916 103719823 103723158
EGU : 105033461 105038775 105051251 105052960 105055421
MUS : 103989287 103996017 135611299 135614727 135616059 135622709 135624958 135626664
ZOF : 121979777 122001068 122002634 122005353 122006876 122017225 122021110 122021257 122024772 122025047 122037934 122041305 122045797 122048809
DCT : 110099730 110102613 110109988 110114781
PEQ : 110018942 110031049 110037465
AOF : 109824017 109831561 109850159
MSIN : 131239844 131244696 131246089
ACAK : 137953342 137954079 137962972
NCOL : 116249268 116251399 116267802
CJF : 131037600 131037627 131079719
SMO : SELMODRAFT_109556(GT17A2) SELMODRAFT_403018(GT17A3) SELMODRAFT_409063(GT17A1)
CSL : COCSUDRAFT_41538 COCSUDRAFT_41853 COCSUDRAFT_66470
NTE : NEUTE1DRAFT125174(NEUTE1DRAFT_125174)
SMP : 10804493(SMAC4_05190)
FVR : FVEG_06710 FVEG_06863
FOX : FOXG_09108 FOXG_09262
FMU : J7337_009372 J7337_009523
TATV : 90961066(TrAtP1_012210)
TASP : 36617270(TrAFT101_010495)
MAW : 19244570(J3458_005039)
MBRN : 26243539(G6M90_00g081230) 26247851(G6M90_00g070740)
CMT : CCM_00141 CCM_00974 CCM_01490 CCM_03026
AMUS : LMH87_006900 LMH87_007920
PLJ : 28883539(PLICBS_000500) 28888650(PLICBS_003820)
PTKZ : JDV02_007803 JDV02_010764
CFJ : CFIO01_01418 CFIO01_07005
CHIG : CH63R_01097 CH63R_04080
CDET : 87939839(CDEST_03336) 87941112(CDEST_04609)
SSL : SS1G_01418 SS1G_02625
BFU : BCIN_02g04100 BCIN_06g01510
PSCO : LY89DRAFT_690955 LY89DRAFT_735198
BGH : BDBG_06575 BDBG_06706
ZTR : MYCGRDRAFT_110873 MYCGRDRAFT_15744
FFU : CLAFUR5_03738 CLAFUR5_11119 CLAFUR5_14290
CBET : CB0940_05440 CB0940_06779
KMG : 30160027(I203_104844)
FME : FOMMEDRAFT_111729 FOMMEDRAFT_96003
ABP : AGABI1DRAFT112787(AGABI1DRAFT_112787)
ABV : AGABI2DRAFT192752(AGABI2DRAFT_192752)
MRR : Moror_14910 Moror_1524
SCM : SCHCO_02631548(SCHCODRAFT_02631548)
TVA : TVAG_2v0082900 TVAG_2v0302120 TVAG_2v0392140 TVAG_2v0628800 TVAG_2v0770450 TVAG_2v1057260
GTT : GUITHDRAFT_107452 GUITHDRAFT_107951 GUITHDRAFT_114812
MPC : Mar181_0482 Mar181_0486
APM : HIMB5_00000730 HIMB5_00012700
BDC : DOE51_07950 DOE51_08860
» show all
Taxonomy
Reference
Authors
Narasimhan S.
Title
Control of glycoprotein synthesis. UDP-GlcNAc:glycopeptide beta 4-N-acetylglucosaminyltransferase III, an enzyme in hen oviduct which adds GlcNAc in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-glycosyl oligosaccharides.
Journal
J Biol Chem 257:10235-42 (1982)
Reference
Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
Journal
Reference
Authors
Brockhausen I, Carver JP, Schachter H
Title
Control of glycoprotein synthesis. The use of oligosaccharide substrates and HPLC to study the sequential pathway for N-acetylglucosaminyltransferases I, II, III, IV, V, and VI in the biosynthesis of highly branched N-glycans by hen oviduct membranes.
Journal
Reference
Authors
Nishikawa A, Ihara Y, Hatakeyama M, Kangawa K, Taniguchi N
Title
Purification, cDNA cloning, and expression of UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III from rat kidney.
Journal
J Biol Chem 267:18199-204 (1992)
Sequence
Reference
Authors
Ihara Y, Nishikawa A, Tohma T, Soejima H, Niikawa N, Taniguchi N
Title
cDNA cloning, expression, and chromosomal localization of human N-acetylglucosaminyltransferase III (GnT-III).
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.4.1.144
LinkDB
All DBs