ID UREF2_BRUA2 Reviewed; 243 AA.
AC Q2YQD7;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 29-MAY-2024, entry version 75.
DE RecName: Full=Urease accessory protein UreF 2 {ECO:0000255|HAMAP-Rule:MF_01385};
GN Name=ureF2 {ECO:0000255|HAMAP-Rule:MF_01385}; OrderedLocusNames=BAB1_1380;
OS Brucella abortus (strain 2308).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
RN [2]
RP LACK OF ROLE IN VIRULENCE.
RX PubMed=17101645; DOI=10.1128/iai.01244-06;
RA Sangari F.J., Seoane A., Rodriguez M.C., Aguero J., Garcia Lobo J.M.;
RT "Characterization of the urease operon of Brucella abortus and assessment
RT of its role in virulence of the bacterium.";
RL Infect. Immun. 75:774-780(2007).
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
CC -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity
CC or pathogen survival in BALB/c mice when administered orally.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01385}.
CC -!- SIMILARITY: Belongs to the UreF family. {ECO:0000255|HAMAP-
CC Rule:MF_01385}.
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DR EMBL; AM040264; CAJ11336.1; -; Genomic_DNA.
DR RefSeq; WP_002966883.1; NZ_KN046823.1.
DR AlphaFoldDB; Q2YQD7; -.
DR SMR; Q2YQD7; -.
DR STRING; 359391.BAB1_1380; -.
DR GeneID; 58775579; -.
DR KEGG; bmf:BAB1_1380; -.
DR PATRIC; fig|359391.11.peg.830; -.
DR HOGENOM; CLU_049215_4_0_5; -.
DR PhylomeDB; Q2YQD7; -.
DR Proteomes; UP000002719; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.4190.10; Urease accessory protein UreF; 1.
DR HAMAP; MF_01385; UreF; 1.
DR InterPro; IPR002639; UreF.
DR InterPro; IPR038277; UreF_sf.
DR PANTHER; PTHR33620; UREASE ACCESSORY PROTEIN F; 1.
DR PANTHER; PTHR33620:SF1; UREASE ACCESSORY PROTEIN F; 1.
DR Pfam; PF01730; UreF; 1.
DR PIRSF; PIRSF009467; Ureas_acces_UreF; 1.
PE 3: Inferred from homology;
KW Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT CHAIN 1..243
FT /note="Urease accessory protein UreF 2"
FT /id="PRO_0000344086"
SQ SEQUENCE 243 AA; 26465 MW; CE0A0602F0527ECB CRC64;
MTMRTATITE FSSSYRSLPG LLHLLQFGDS ALPIGGFSFS NGLESAIQQN LVHDKETLRE
FTLTAMNQAA TSDGIALLTA HRAARADDRG ALQVIDKAVF ERKLNEETRL MTVRMGRKLC
ELSASIIDDR LNRDWLECIK TAETPGTHPV SLGLAFAALD VDGRDAFGAQ QYGVATTILG
AALRLMRVSF MDTQKILLEA TSTVAPAYEE IADAGIEDMA SFAPMVDILA AVHVKGHVRM
FMN
//
