ID PTR41_ARATH Reviewed; 548 AA.
AC Q9M173; Q8GZ59;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 02-OCT-2024, entry version 127.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.4;
DE Short=AtNPF2.4;
DE AltName: Full=Probable nitrate excretion transporter 5;
DE AltName: Full=Protein NAXT1-like 4;
GN Name=NPF2.4; OrderedLocusNames=At3g45700; ORFNames=T6D9.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [6]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=17993627; DOI=10.1105/tpc.106.048173;
RA Segonzac C., Boyer J.C., Ipotesi E., Szponarski W., Tillard P.,
RA Touraine B., Sommerer N., Rossignol M., Gibrat R.;
RT "Nitrate efflux at the root plasma membrane: identification of an
RT Arabidopsis excretion transporter.";
RL Plant Cell 19:3760-3777(2007).
RN [7]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Transporter involved in a passive nitrate efflux.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in the root stele.
CC {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:17993627}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AL157735; CAB75783.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78061.1; -; Genomic_DNA.
DR EMBL; AK117193; BAC41869.1; -; mRNA.
DR EMBL; BT005911; AAO64846.1; -; mRNA.
DR PIR; T47510; T47510.
DR RefSeq; NP_190156.1; NM_114439.3.
DR AlphaFoldDB; Q9M173; -.
DR SMR; Q9M173; -.
DR BioGRID; 9032; 5.
DR IntAct; Q9M173; 5.
DR STRING; 3702.Q9M173; -.
DR PaxDb; 3702-AT3G45700.1; -.
DR ProteomicsDB; 226440; -.
DR EnsemblPlants; AT3G45700.1; AT3G45700.1; AT3G45700.
DR GeneID; 823712; -.
DR Gramene; AT3G45700.1; AT3G45700.1; AT3G45700.
DR KEGG; ath:AT3G45700; -.
DR Araport; AT3G45700; -.
DR TAIR; AT3G45700; NPF2.4.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9M173; -.
DR OMA; NDDHEQD; -.
DR OrthoDB; 1210137at2759; -.
DR PhylomeDB; Q9M173; -.
DR PRO; PR:Q9M173; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M173; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015108; F:chloride transmembrane transporter activity; IDA:TAIR.
DR GO; GO:1902476; P:chloride transmembrane transport; IDA:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0006857; P:oligopeptide transport; IEA:InterPro.
DR CDD; cd17416; MFS_NPF1_2; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; OLIGOPEPTIDE TRANSPORTER-RELATED; 1.
DR PANTHER; PTHR11654:SF327; PROTEIN NRT1_ PTR FAMILY 2.1-RELATED; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR PROSITE; PS01022; PTR2_1; 1.
PE 2: Evidence at transcript level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..548
FT /note="Protein NRT1/ PTR FAMILY 2.4"
FT /id="PRO_0000399975"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 10
FT /note="E -> D (in Ref. 3; BAC41869 and 4; AAO64846)"
FT /evidence="ECO:0000305"
FT CONFLICT 328
FT /note="A -> V (in Ref. 3; BAC41869 and 4; AAO64846)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 548 AA; 60828 MW; 5B7A75848915EB2B CRC64;
MANSDSGDKE AHRSSKHGGW ITLPFMLVTL LGMSITYFGW VMNLIVFLIE EFNIKSIAAV
QISNIVNGVV NMLPVVAAIL ADSFFGNIPV ISASAFISLT GISLLTLIAS LDYLRPRPCE
TGSILCQSPS KLQLGILYAA LALVITGTAG TRFILASAGA NQYKKPKEQG RFFNWYFFTL
YGGAITGTTA IVYAQDNASW KLGFGLCVAA NLISFIIFVA GKRLYEHDQP LGSPYTSLVR
VLVAATMKRK AVISYKDEDY HHRELEKETK TYVAMPSKSF RFLNRAALKT EGDSNNNMWR
LCSVQEVEDF KAVLRLVPLW TSVMFLSAPL AVQMSMTVLQ AMVMDRKLGP HFKVSAGSMQ
VIALVSGCVF IILNNWTTYP MYQKLIRKPL TPLQKVGIGH VLTILSMAIS AVVEAKRLKT
VENSHLMSVL WLVPALVING IGEAFHFPAN IAIFYGEFPE SLRNTATSLT SVVMGISFYL
STALIDVIQR TTKWLPNDIN HGRVDNVYLV LVIIGVSNFG YFLVCSWFYK YRNLKNDDHE
QDLKDVTN
//
