ID NAS3_ARATH Reviewed; 320 AA.
AC O80483;
DT 13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 27-NOV-2024, entry version 141.
DE RecName: Full=Nicotianamine synthase 3;
DE EC=2.5.1.43;
DE AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 3;
DE Short=AtNAS3;
GN Name=NAS3; OrderedLocusNames=At1g09240; ORFNames=T12M4.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Suzuki K., Higuchi K., Nakanishi H., Nishizawa N.-K., Mori S.;
RT "Cloning of nicotianamine synthase genes from Arabidopsis thaliana.";
RL Soil Sci. Plant Nutr. 45:993-1002(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Synthesizes nicotianamine, a polyamine which serves as a
CC sensor for the physiological iron status within the plant, and/or might
CC be involved in the transport of iron.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-
CC thioadenosine + 3 H(+); Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC -!- TISSUE SPECIFICITY: In shoots.
CC -!- INDUCTION: Constitutively expressed.
CC -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC {ECO:0000305}.
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DR EMBL; AB021936; BAA74591.1; -; Genomic_DNA.
DR EMBL; AC003114; AAC24082.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28417.1; -; Genomic_DNA.
DR EMBL; AY140031; AAM98172.1; -; mRNA.
DR EMBL; BT008817; AAP68256.1; -; mRNA.
DR EMBL; AY087620; AAM65161.1; -; mRNA.
DR PIR; C86225; C86225.
DR RefSeq; NP_172395.1; NM_100794.4.
DR AlphaFoldDB; O80483; -.
DR SMR; O80483; -.
DR STRING; 3702.O80483; -.
DR PaxDb; 3702-AT1G09240.1; -.
DR ProteomicsDB; 251095; -.
DR EnsemblPlants; AT1G09240.1; AT1G09240.1; AT1G09240.
DR GeneID; 837444; -.
DR Gramene; AT1G09240.1; AT1G09240.1; AT1G09240.
DR KEGG; ath:AT1G09240; -.
DR Araport; AT1G09240; -.
DR TAIR; AT1G09240; NAS3.
DR eggNOG; ENOG502QTU6; Eukaryota.
DR HOGENOM; CLU_031919_1_1_1; -.
DR InParanoid; O80483; -.
DR OMA; DTIFHNF; -.
DR OrthoDB; 2718460at2759; -.
DR PhylomeDB; O80483; -.
DR BioCyc; ARA:AT1G09240-MONOMER; -.
DR BRENDA; 2.5.1.43; 399.
DR PRO; PR:O80483; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80483; baseline and differential.
DR GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR GO; GO:0010233; P:phloem transport; IGI:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR FunFam; 3.40.50.150:FF:000182; Nicotianamine synthase; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR004298; Nicotian_synth.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR32266; NICOTIANAMINE SYNTHASE 3; 1.
DR PANTHER; PTHR32266:SF12; NICOTIANAMINE SYNTHASE 3; 1.
DR Pfam; PF03059; NAS; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51142; NAS; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..320
FT /note="Nicotianamine synthase 3"
FT /id="PRO_0000212702"
SQ SEQUENCE 320 AA; 35751 MW; 78927358CDD9E58E CRC64;
MGCQDEQLVQ TICDLYEKIS KLESLKPSED VNILFKQLVS TCIPPNPNID VTKMCDRVQE
IRLNLIKICG LAEGHLENHF SSILTSYQDN PLHHLNIFPY YNNYLKLGKL EFDLLEQNLN
GFVPKSVAFI GSGPLPLTSI VLASFHLKDT IFHNFDIDPS ANSLASLLVS SDPDISQRMF
FHTVDIMDVT ESLKSFDVVF LAALVGMNKE EKVKVIEHLQ KHMAPGAVLM LRSAHGPRAF
LYPIVEPCDL QGFEVLSIYH PTDDVINSVV ISKKHPVVSI GNVGGPNSCL LKPCNCSKTH
AKMNKNMMIE EFGAREEQLS
//
