ID NAS1_ORYSI Reviewed; 332 AA.
AC A2XFU4; Q9SXQ7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 27-NOV-2024, entry version 70.
DE RecName: Full=Nicotianamine synthase 1;
DE EC=2.5.1.43;
DE AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 1;
DE Short=OsNAS1;
GN Name=NAS1; ORFNames=OsI_010937;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=cv. IR36; TISSUE=Root;
RX PubMed=11169192; DOI=10.1046/j.1365-313x.2001.00951.x;
RA Higuchi K., Watanabe S., Takahashi M., Kawasaki S., Nakanishi H.,
RA Nishizawa N.-K., Mori S.;
RT "Nicotianamine synthase gene expression differs in barley and rice under
RT Fe-deficient conditions.";
RL Plant J. 25:159-167(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Guang-Lu-Ai No.4;
RX PubMed=17522955; DOI=10.1007/s11103-007-9174-7;
RA Liu X., Lu T., Yu S., Li Y., Huang Y., Huang T., Zhang L., Zhu J., Zhao Q.,
RA Fan D., Mu J., Shangguan Y., Feng Q., Guan J., Ying K., Zhang Y., Lin Z.,
RA Sun Z., Qian Q., Lu Y., Han B.;
RT "A collection of 10,096 indica rice full-length cDNAs reveals highly
RT expressed sequence divergence between Oryza sativa indica and japonica
RT subspecies.";
RL Plant Mol. Biol. 65:403-415(2007).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=14617093; DOI=10.1046/j.1365-313x.2003.01878.x;
RA Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K.;
RT "Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are
RT expressed in cells involved in long-distance transport of iron and
RT differentially regulated by iron.";
RL Plant J. 36:366-381(2003).
CC -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC intermediate in the synthesis of the phytosiderophores of the mugineic
CC acid type found in gramineae which serve as a sensor for the
CC physiological iron status within the plant, and/or might be involved in
CC the transport of iron. {ECO:0000269|PubMed:11169192,
CC ECO:0000269|PubMed:14617093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 S-adenosyl-L-methionine = nicotianamine + 3 S-methyl-5'-
CC thioadenosine + 3 H(+); Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:11169192,
CC ECO:0000269|PubMed:14617093}.
CC -!- INDUCTION: By iron deficiency in roots and chlorotic leaves.
CC {ECO:0000269|PubMed:11169192, ECO:0000269|PubMed:14617093}.
CC -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC {ECO:0000305}.
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DR EMBL; AB021746; BAA74588.2; -; mRNA.
DR EMBL; AB046401; BAB17825.1; -; Genomic_DNA.
DR EMBL; CM000128; EAY89704.1; -; Genomic_DNA.
DR EMBL; CT829820; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; A2XFU4; -.
DR SMR; A2XFU4; -.
DR STRING; 39946.A2XFU4; -.
DR HOGENOM; CLU_031919_0_0_1; -.
DR BioCyc; MetaCyc:MONOMER-13933; -.
DR BRENDA; 2.5.1.43; 11590.
DR Proteomes; UP000007015; Chromosome 3.
DR GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR004298; Nicotian_synth.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR32266:SF10; NICOTIANAMINE SYNTHASE 2; 1.
DR PANTHER; PTHR32266; NICOTIANAMINE SYNTHASE 3; 1.
DR Pfam; PF03059; NAS; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51142; NAS; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..332
FT /note="Nicotianamine synthase 1"
FT /id="PRO_0000300238"
FT CONFLICT 91
FT /note="N -> S (in Ref. 2; EAY89704)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="G -> E (in Ref. 2; EAY89704)"
FT /evidence="ECO:0000305"
FT CONFLICT 235
FT /note="S -> T (in Ref. 1; BAA74588/BAB17825)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="P -> L (in Ref. 2; EAY89704)"
FT /evidence="ECO:0000305"
FT CONFLICT 252
FT /note="V -> I (in Ref. 2; EAY89704)"
FT /evidence="ECO:0000305"
FT CONFLICT 263
FT /note="C -> Y (in Ref. 2; EAY89704)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="E -> D (in Ref. 2; EAY89704)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 332 AA; 34933 MW; F6A8115693D60BF3 CRC64;
MEAQNQEVAA LVEKIAGLHA AISKLPSLSP SAEVDALFTD LVTACVPASP VDVAKLGPEA
QAMREELIRL CSAAEGHLEA HYADMLAAFD NPLDHLARFP YYGNYVNLSK LEYDLLVRYV
PGIAPTRVAF VGSGPLPFSS LVLAAHHLPD AVFDNYDRCG AANERARRLF RGADEGLGAR
MAFHTADVAT LTGELGAYDV VFLAALVGMA AEEKAGVIAH LGAHMADGAA LVVRSAHGAR
GFLYPIVDPE DVRRGGFDVL AVCHPEDEVI NSVIVARKVG AAAAAAAARR DELADSRGVV
LPVVGPPSTC CKVEASAVEK AEEFAANKEL SV
//
