ID DPOA2_DICDI Reviewed; 623 AA.
AC Q54S08;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 19-JAN-2010, sequence version 2.
DT 27-NOV-2024, entry version 91.
DE RecName: Full=DNA polymerase alpha subunit B;
GN Name=polA2; ORFNames=DDB_0232277;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: May play an essential role at the early stage of chromosomal
CC DNA replication by coupling the polymerase alpha/primase complex to the
CC cellular replication machinery. {ECO:0000250}.
CC -!- SUBUNIT: DNA polymerase alpha:primase is a four subunit enzyme complex,
CC which is assembled throughout the cell cycle, and consists of the two
CC DNA polymerase subunits A and B, and the DNA primase large and small
CC subunits. Subunit B binds to subunit A (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA polymerase alpha subunit B family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000047; EAL66225.2; -; Genomic_DNA.
DR RefSeq; XP_640231.2; XM_635139.2.
DR AlphaFoldDB; Q54S08; -.
DR SMR; Q54S08; -.
DR STRING; 44689.Q54S08; -.
DR PaxDb; 44689-DDB0304676; -.
DR EnsemblProtists; EAL66225; EAL66225; DDB_G0282731.
DR GeneID; 8623771; -.
DR KEGG; ddi:DDB_G0282731; -.
DR dictyBase; DDB_G0282731; polA2.
DR VEuPathDB; AmoebaDB:DDB_G0282731; -.
DR eggNOG; KOG1625; Eukaryota.
DR HOGENOM; CLU_439059_0_0_1; -.
DR InParanoid; Q54S08; -.
DR OMA; MGPFIDE; -.
DR PhylomeDB; Q54S08; -.
DR Reactome; R-DDI-113501; Inhibition of replication initiation of damaged DNA by RB1/E2F1.
DR Reactome; R-DDI-68952; DNA replication initiation.
DR Reactome; R-DDI-68962; Activation of the pre-replicative complex.
DR Reactome; R-DDI-69091; Polymerase switching.
DR Reactome; R-DDI-69166; Removal of the Flap Intermediate.
DR Reactome; R-DDI-69183; Processive synthesis on the lagging strand.
DR PRO; PR:Q54S08; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR FunFam; 3.60.21.60:FF:000016; DNA polymerase alpha subunit B-like; 1.
DR Gene3D; 3.60.21.60; -; 1.
DR InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR InterPro; IPR016722; DNA_pol_alpha_bsu.
DR PANTHER; PTHR23061; DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT; 1.
DR PANTHER; PTHR23061:SF12; DNA POLYMERASE ALPHA SUBUNIT B; 1.
DR Pfam; PF04042; DNA_pol_E_B; 1.
PE 3: Inferred from homology;
KW DNA replication; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..623
FT /note="DNA polymerase alpha subunit B"
FT /id="PRO_0000371219"
FT REGION 113..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 623 AA; 70657 MW; 3D390599E77655BA CRC64;
MDSSLFSLKN SFGGFNDEEL LRVQKIIEDL ELTESIVSKE WVTFALQKKI AEDYSKHLDP
FKVFLKKNIK KLKVQKTTEQ IKEVAEEDPK MFENVEIEDI IFDTDSLVDE SEIPKIKDEP
SSSVDVSTAR NKNNHNNNNN NNPSLPNKSM FKPTKQEMTN DNIKKVVIAD DIGDFDFTTA
NQPIKQSNPT QLLNYKERKN IGGISYSMNN NEELKTDLSL REKKSCDLKG AGEEFYPIKY
HRDTLEQRTK ILKESVQSNN YTFPVSLDCQ DDESIVRSVG RVWMYDQETI LSKRFYLLGN
ERDSSSSGGS SGSASIFSLE NNIPDYSMFS GQVVMAESKK IAGSHFYYTN KLYTPNPLPF
FSQRKECGDI NVMIASGPFD LQKSTPDYTP LDDLCNVVSN KKPHILFLMG PFIDESNVHI
KKYSETFSEL FNNLMKKLND NIPSTTKVLI VPSLNDVDHE YIFPQPPYVP SVNLNSNILF
VPNPFTLIIN ESFTIGITSS DIYNNLVSKA HFKNKHTPED IFNMIINQNN YYPMHPAQAP
ISMRYLQHLN FPGFTPDILV VPKNSPIAAI SNDVLCLGVP PIVGNKSTFG SYAELTVTKS
KQSLAFDNNI PVSKRTIVNF KNI
//
