ID CER7L_ARATH Reviewed; 307 AA.
AC Q9LDM2; Q8LGD7;
DT 13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 27-NOV-2024, entry version 146.
DE RecName: Full=Exosome complex component RRP45A {ECO:0000305};
DE AltName: Full=Protein ECERIFERUM 7;
DE AltName: Full=RRP45 homolog A {ECO:0000305};
DE AltName: Full=Ribosomal RNA-processing protein 45A {ECO:0000305};
DE Short=AtRRPA5a {ECO:0000303|PubMed:17351114};
GN Name=RRP45A {ECO:0000305}; OrderedLocusNames=At3g12990; ORFNames=MGH6.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17351114; DOI=10.1105/tpc.106.049304;
RA Hooker T.S., Lam P., Zheng H., Kunst L.;
RT "A core subunit of the RNA-processing/degrading exosome specifically
RT influences cuticular wax biosynthesis in Arabidopsis.";
RL Plant Cell 19:904-913(2007).
CC -!- FUNCTION: Probable 3'->5' exoribonuclease involved in the regulation of
CC cuticular wax biosynthesis. Can perform exosomal functions and
CC partially complement the yeast rrp45 null mutant.
CC {ECO:0000269|PubMed:17351114}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17351114}. Nucleus
CC {ECO:0000269|PubMed:17351114}. Note=Excluded from the nucleolus.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, buds and
CC siliques.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:17351114}.
CC -!- SIMILARITY: Belongs to the RNase PH family. {ECO:0000305}.
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DR EMBL; AB026645; BAB02509.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75271.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75273.1; -; Genomic_DNA.
DR EMBL; BT003077; AAO23642.1; -; mRNA.
DR EMBL; AK227231; BAE99268.1; -; mRNA.
DR EMBL; AY084328; AAM60913.1; -; mRNA.
DR RefSeq; NP_001189874.1; NM_001202945.2.
DR RefSeq; NP_566441.1; NM_112136.5.
DR AlphaFoldDB; Q9LDM2; -.
DR SMR; Q9LDM2; -.
DR BioGRID; 5819; 13.
DR STRING; 3702.Q9LDM2; -.
DR PaxDb; 3702-AT3G12990.1; -.
DR ProteomicsDB; 220544; -.
DR DNASU; 820485; -.
DR EnsemblPlants; AT3G12990.1; AT3G12990.1; AT3G12990.
DR EnsemblPlants; AT3G12990.3; AT3G12990.3; AT3G12990.
DR GeneID; 820485; -.
DR Gramene; AT3G12990.1; AT3G12990.1; AT3G12990.
DR Gramene; AT3G12990.3; AT3G12990.3; AT3G12990.
DR KEGG; ath:AT3G12990; -.
DR Araport; AT3G12990; -.
DR TAIR; AT3G12990; RRP45A.
DR eggNOG; KOG1614; Eukaryota.
DR HOGENOM; CLU_038194_1_0_1; -.
DR InParanoid; Q9LDM2; -.
DR OMA; HPPHERE; -.
DR OrthoDB; 128403at2759; -.
DR PhylomeDB; Q9LDM2; -.
DR PRO; PR:Q9LDM2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LDM2; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0000178; C:exosome (RNase complex); IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0000175; F:3'-5'-RNA exonuclease activity; ISS:TAIR.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd11368; RNase_PH_RRP45; 1.
DR FunFam; 3.30.230.70:FF:000007; Exosome complex component RRP45B; 1.
DR Gene3D; 3.30.230.70; GHMP Kinase, N-terminal domain; 1.
DR InterPro; IPR001247; ExoRNase_PH_dom1.
DR InterPro; IPR015847; ExoRNase_PH_dom2.
DR InterPro; IPR036345; ExoRNase_PH_dom2_sf.
DR InterPro; IPR050590; Exosome_comp_Rrp42_subfam.
DR InterPro; IPR027408; PNPase/RNase_PH_dom_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR033100; Rrp45.
DR PANTHER; PTHR11097:SF14; EXOSOME COMPLEX COMPONENT RRP45; 1.
DR PANTHER; PTHR11097; EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN; 1.
DR Pfam; PF01138; RNase_PH; 1.
DR Pfam; PF03725; RNase_PH_C; 1.
DR SUPFAM; SSF55666; Ribonuclease PH domain 2-like; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..307
FT /note="Exosome complex component RRP45A"
FT /id="PRO_0000424436"
FT CONFLICT 290
FT /note="K -> E (in Ref. 5; AAM60913)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 307 AA; 33875 MW; 832434D78EF05FAF CRC64;
MEGRLNNMWR LTVNESKFVE SALQSELRVD GRGLYDYRKL TIKFGKEYGS SQVQLGQTHV
MAFVTAQLVQ PYKDRPSEGS FSIFTEFSPM ADPSFEPGHP GESAVELGRI IDRALRESRA
VDTESLCVLA GKLVWSVRID LHILDNGGNL VDAANVAALA ALMTFRRPDC TVGGDNSQDV
IIHPPEEREP LPLIIHHLPI AFTFGFFNKG SILVMDPTYV EEAVMCGRMT VTVNANGDIC
AIQKPGEEGV NQSVILHCLR LASSRASATT KIIRDAVEAY NRERSSQKVK RHHTLAKSEV
LGPIVVV
//
