› 2a66

X-ray diffraction
2.2Å resolution

Human Liver Receptor Homologue DNA-Binding Domain (hLRH-1 DBD) in Complex with dsDNA from the hCYP7A1 Promoter

Released:
DOI: 10.2210/pdb2a66/pdb
PDB entry 2a66 coloured by chain, front view.
PDB entry 2a66 coloured by chain, side view.
PDB entry 2a66 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human LRH-1 DBD-DNA complex reveals Ftz-F1 domain positioning is required for receptor activity.
Solomon IH, Hager JM, Safi R, McDonnell DP, Redinbo MR, Ortlund EA
J Mol Biol 354 1091-102 (2005)
PMID: 16289203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115062 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 5 group A member 2 Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 13.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00482 (Residues: 79-187; Coverage: 20%)
Gene names: B1F, CPF, FTF, NR5A2
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*GP*TP*TP*CP*AP*AP*GP*GP*CP*CP*AP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Homo sapiens
5'-D(*CP*TP*GP*GP*CP*CP*TP*TP*GP*AP*AP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.64 KDa
Source organism: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43
Unit cell:
a: 40.77Å b: 40.77Å c: 104.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.226
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The nuclear receptor superfamily: A structural perspective.
Weikum et al. (2018)
8 more

6 mentions without citation

Wide spectrum of NR5A1-related phenotypes in 46,XY and 46,XX individuals.
Domenice et al. (2016)
5 more