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HEPES

HEPES Structure
HEPES structure
CAS No.
7365-45-9
Chemical Name:
HEPES
Synonyms
HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
CBNumber:
CB5408557
Molecular Formula:
C8H18N2O4S
Molecular Weight:
238.3
MDL Number:
MFCD00006158
MOL File:
7365-45-9.mol
MSDS File:
SDS
Last updated:2024-11-20 11:41:24
Request For Quotation

HEPES Properties

Melting point 234-238 °C
Boiling point 408℃[at 101 325 Pa]
Density 1.07 g/mL at 20 °C
vapor pressure 0Pa at 25℃
refractive index n20/D 1.339
storage temp. 2-8°C
solubility H2O: 1 M at 20 °C, clear, colorless
form powder
color White
PH Range 7.0 - 7.6
PH 5.0-6.5 (1Maqueous solution)
pka 7.5(at 25℃)
Water Solubility Soluble
λmax λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 14,4654
BRN 883043
Stability Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP -3.85 at 20℃
CAS DataBase Reference 7365-45-9(CAS DataBase Reference)
EWG's Food Scores 1
FDA UNII RWW266YE9I
EPA Substance Registry System 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H335
Precautionary statements  P261-P271-P280
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  24/25-22-36-26
WGK Germany  1
RTECS  TL6809000
TSCA  Yes
HS Code  29335995
NFPA 704
1
2 1

HEPES price More Price(151)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich PHG0001 HEPES 7365-45-9 1KIT $978 2024-03-01 Buy
Sigma-Aldrich H4034 HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture 7365-45-9 25KG $6140 2024-03-01 Buy
Sigma-Aldrich H3375 HEPES ≥99.5% (titration) 7365-45-9 25KG $5870 2024-03-01 Buy
Sigma-Aldrich H3375 HEPES ≥99.5% (titration) 7365-45-9 50KG $11060 2024-03-01 Buy
Sigma-Aldrich 83264 HEPES buffer solution 1?M in H2O 7365-45-9 100ML $67.1 2024-03-01 Buy
Product number Packaging Price Buy
PHG0001 1KIT $978 Buy
H4034 25KG $6140 Buy
H3375 25KG $5870 Buy
H3375 50KG $11060 Buy
83264 100ML $67.1 Buy

HEPES Chemical Properties,Uses,Production

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Uses

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

Uses

HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay. 

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

HEPES synthesis

103-76-4
1562-00-1
7365-45-9
Synthesis of HEPES from 1-(2-Hydroxyethyl)piperazine and Sodium isethionate

HEPES Preparation Products And Raw materials

Raw materials

1-(2-Hydroxyethyl)piperazine Sodium isethionate

Preparation Products

HEPES Suppliers

Global( 734)Suppliers
Supplier Tel Email Country ProdList Advantage
Tangshan Moneide Trading Co., Ltd.
+86-315-8309571 +8615633399667 [email protected] China 574 58
LEAP CHEM CO., LTD.
+86-852-30606658 [email protected] China 24727 58
Jiangsu Boquan Biotechnology Co., Ltd.
+86-18168774353 [email protected] China 248 58
Hebei Chuanghai Biotechnology Co,.LTD
+86-13131129325 [email protected] China 5889 58
Suzhou Genemill Biotechnology Co.,Ltd
+8615862502491 [email protected] China 39 58
Hebei Weibang Biotechnology Co., Ltd 		+8615531157085 [email protected] China 8810 58
Henan Bao Enluo International TradeCo.，LTD 		+86-17331933971 +86-17331933971 [email protected] China 2472 58
Shaanxi Haibo Biotechnology Co., Ltd 		+undefined18602966907 [email protected] China 997 58
Anhui Ruihan Technology Co., Ltd 		+8617756083858 [email protected] China 973 58
Chongqing Zhihe Biopharmaceutical Co., Ltd. 		+86-18580541567; +8618580541567 [email protected] China 302 58
Supplier Advantage
Tangshan Moneide Trading Co., Ltd.
58
LEAP CHEM CO., LTD.
58
Jiangsu Boquan Biotechnology Co., Ltd.
58
Hebei Chuanghai Biotechnology Co,.LTD
58
Suzhou Genemill Biotechnology Co.,Ltd
58
Hebei Weibang Biotechnology Co., Ltd 		58
Henan Bao Enluo International TradeCo.，LTD 		58
Shaanxi Haibo Biotechnology Co., Ltd 		58
Anhui Ruihan Technology Co., Ltd 		58
Chongqing Zhihe Biopharmaceutical Co., Ltd. 		58

Related articles

View Lastest Price from HEPES manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
HEPES pictures 2024-12-16 HEPES
7365-45-9
 US $25.00 / kg 1kg 99% 5000kg/week Hebei Zhuanglai Chemical Trading Co.,Ltd
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid pictures 2024-12-16 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
7365-45-9
 US $0.00 / Kg/Drum 1KG 99% 200mt Jinan Finer Chemical Co., Ltd
HEPES pictures 2024-12-16 HEPES
7365-45-9
 US $0.00-0.00 / kg 1kg 99% 20MT Watson Biotechnology Co.,Ltd
  • HEPES pictures
  • HEPES
    7365-45-9
  • US $25.00 / kg
  • 99%
  • Hebei Zhuanglai Chemical Trading Co.,Ltd
  • HEPES pictures
  • HEPES
    7365-45-9
  • US $0.00-0.00 / kg
  • 99%
  • Watson Biotechnology Co.,Ltd

HEPES Spectrum

HEPES(7365-45-9)MSHEPES(7365-45-9)IR1HEPES(7365-45-9)IR2HEPES(7365-45-9)Raman

7365-45-9(HEPES)Related Search:

2-Ethylpiperazine 1-Ethylpiperazine 2-Hydroxyethanesulphonic acid 2-(METHYLSULFONYL)ETHANOL Ethyl 2-(Chlorosulfonyl)acetate
N-Aminoethylpiperazine 2-Piperidinoethanol Hydroxyethyl starch Piperazine Sodium 2-bromoethanesulphonate
Hydroxyethyl Cellulose Calcium dobesilate 1-(2-Hydroxyethyl)piperazine 2-(2-Aminoethylamino)ethanol Sodium isethionate
Folic acid Piperazine citrate Ascoric Acid Hepes Buffer Hepes Buffered Saline
Hepes Solution

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HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+% N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID)) Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz 1-HeptaneSulfonicAcidSod.SaltGr N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid) N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes) Hepes,LowSodium,FreeAcid HEPES free acid, DNase, Rnase and Protease free, for molecular biology, 99+% Hydroxyethyl)-1-piperazineethyanesulfonic acid, 4-(2- 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES) 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid HEPES, for biochemistry, 99% 4-(2-Hydroxyethyl)-1-Piperazin Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- hepes solution HEPES,BIOLOGICALBUFFER HEPES,BIOLOGICALBUFFER,ULTRAPURE N-(2-hydroxymethyl)piperazine-N｀-(2-ethanesulfonic acid)(HEPES) N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES) 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES HEPES / 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULPHONIC ACID HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID) HEPES ULTRAPURE N-2-HYDROXYETHYLPIPERAZINE-N''-2-ETHANE SULPHONIC ACID >99.5% N-[2-HYDROXYERHYL]PIPERAZINE-N''-ETHANESULFONICACID(HEPES) HEPES free acid ultrapure N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid, low sodium >99.5% 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research] HEPES(N-[2-Hydroxyethyl]piperazine-N'-[Ethanesulfonic acid]),Free Acid N'-2-N-2-hydroxyethyl piperazineethanesulfonic acid HEPES, >=99.5% (titration) HEPES SynonyMs 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid HEPES Buffer Solution (1M) HEPES,4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid HEPES Vetec(TM) reagent grade, 99.5% 4-CHLORO-1-NAPHTHOL ULTRA PURE GRADE POTASSIUM HYDROXIDE 0,05 MOL DUTY PAID POTASSIUM HYDROXIDE 0,5MOL/L DUTY PAID POTASSIUM HYDROXIDE 1 MOL/L DUTY PAID SELENIUM STAND MALATE DEHYDROGENASE ULTRA PURE GRADE PHOSPHOTUNGSTIC ACID HIGH PURITY GRADE N-[2-HYDROXYETHYL]PIPERAZINE-N'-[2-ETHANESULFONIC ACID];4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci hepesfreeacid 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID LABOTEST-BB LT00138109 LABOTEST-BB LT00233156 HEPES HEPES-ACID HEPES BUFFER